+ All Categories
Home > Documents > Biology 22 Drosophila Identification of various phenotypes.

Biology 22 Drosophila Identification of various phenotypes.

Date post: 17-Jan-2016
Category:
Upload: ariel-whitehead
View: 216 times
Download: 2 times
Share this document with a friend
Popular Tags:
30
Biology 22 Drosophila Drosophila Identification of various Identification of various phenotypes. phenotypes.
Transcript
Page 1: Biology 22 Drosophila Identification of various phenotypes.

Biology 22

DrosophilaDrosophila

Identification of various Identification of various phenotypes.phenotypes.

Page 2: Biology 22 Drosophila Identification of various phenotypes.

Reference for Pictures:

Carolina Carolina Drosophila melanogsterDrosophila melanogster Manual Manual (45-2620)(45-2620)

Published: Carolina Biological Supply Published: Carolina Biological Supply CompanyCompany

Page 3: Biology 22 Drosophila Identification of various phenotypes.

The life C

ycle

Page 4: Biology 22 Drosophila Identification of various phenotypes.

Life Cycle: Drosophila melanogaster

Eggs: contain filaments Eggs: contain filaments

2 molting phases “instars”2 molting phases “instars”

Life cycle is 10 days Life cycle is 10 days

Page 5: Biology 22 Drosophila Identification of various phenotypes.

Fruity Fly Egg and Larva

Page 6: Biology 22 Drosophila Identification of various phenotypes.

Fruit Fly Pupa and Adult

Page 7: Biology 22 Drosophila Identification of various phenotypes.

Identification of:

Gender

Body Color

Page 8: Biology 22 Drosophila Identification of various phenotypes.

Wild Type Versus Mutants

Body ColorBody Color

Wild type: BeigeWild type: Beige Mutant: Yellow bodyMutant: Yellow body

Page 9: Biology 22 Drosophila Identification of various phenotypes.

Parental phenotypes Experiment: body color

A= female: wt (beige)A= female: wt (beige) B=female: wt (beige)B=female: wt (beige) C=male: wt (beige)C=male: wt (beige) D=female: mutant (yellow)D=female: mutant (yellow) E=male: mutant (yellow)E=male: mutant (yellow)

Page 10: Biology 22 Drosophila Identification of various phenotypes.

Fruit Fly Body Color

Page 11: Biology 22 Drosophila Identification of various phenotypes.

Sex combs: Male Drosophila

Page 12: Biology 22 Drosophila Identification of various phenotypes.

Abdominal segements

7 segments to female abdominal segments7 segments to female abdominal segments

5 segments to male abdominal segments5 segments to male abdominal segments

Male body size is smaller than femaleMale body size is smaller than female

But the “last” abdominal segment is larger in maleBut the “last” abdominal segment is larger in male

Page 13: Biology 22 Drosophila Identification of various phenotypes.

Nap TimeTriethylamine

Page 14: Biology 22 Drosophila Identification of various phenotypes.

How to anesthetize a fly

Page 15: Biology 22 Drosophila Identification of various phenotypes.

How to use the Napper

Page 16: Biology 22 Drosophila Identification of various phenotypes.

View the Fruit Fly

Page 17: Biology 22 Drosophila Identification of various phenotypes.

You will record class data

After counting the crosses record the data After counting the crosses record the data on the class spreadsheet on the class spreadsheet

Page 18: Biology 22 Drosophila Identification of various phenotypes.
Page 19: Biology 22 Drosophila Identification of various phenotypes.

3 point test cross: Experiment 2

Experiment 2Experiment 2 3 point test cross3 point test cross

Eye colorEye color Body colorBody color Wing lengthWing length

Page 20: Biology 22 Drosophila Identification of various phenotypes.

3 point test cross for:

1. Body Color1. Body Color

2. Wing length2. Wing length

3. Eye Color3. Eye Color

♦♦ Wt vs. Mutant

Beige (bl+) vs. Black (bl)

Long(vg+) vs. Vestigal(vg)

Brick red (br+) vs. brown (br)

Page 21: Biology 22 Drosophila Identification of various phenotypes.

Crosses:

Female Female Males Males

Males and female progeny from this F2 cross Males and female progeny from this F2 cross are your 3 point test cross results!are your 3 point test cross results!

Xbl vg bw

bl vg bw

bl+ vg+ Bw+

bl vg bw

Page 22: Biology 22 Drosophila Identification of various phenotypes.

There are all sorts of eyes!

Page 23: Biology 22 Drosophila Identification of various phenotypes.

Eyes

Page 24: Biology 22 Drosophila Identification of various phenotypes.

You will have only red eyes (wt)

Page 25: Biology 22 Drosophila Identification of various phenotypes.

Fruit Fly Wings

Page 26: Biology 22 Drosophila Identification of various phenotypes.

1866-1945

Page 27: Biology 22 Drosophila Identification of various phenotypes.
Page 28: Biology 22 Drosophila Identification of various phenotypes.
Page 29: Biology 22 Drosophila Identification of various phenotypes.

Parental phenotypes

A= female: wt beige bodyA= female: wt beige body B=female: wt beige bodyB=female: wt beige body C=male: wt beige bodyC=male: wt beige body D=female: yellow bodyD=female: yellow body E=male: yellow bodyE=male: yellow body

Page 30: Biology 22 Drosophila Identification of various phenotypes.

Female and Male Fruit Flies


Recommended