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Biomarkers in Terrestrial Ecotoxicology: Stress Ecology in Bees

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Biomarkers in Terrestrial Ecotoxicology: Stress Ecology in Bees Chair: Dr. Fábio Camargo Abdalla, UFSCar – Sorocaba, [email protected] “State of art of morphological biomarkers in ecotoxicology researche on bees” Speaker 1: Caio Eduardo da C. Domingues - Post-graduation in Biotechnology & Environmental Monitoring “Fat body, pericardial cells and hemocytes in association with the myogenic region: a model system to analysis the stress ecology in biomarkers of bees” Speaker 2: Paulo José Balsamo – Post-graduation in Biotechnology & Environmental Monitoring “An old subject, but a new challenge in ecotoxicology applied to the study of morphological biomarkers in bees: Hormesis” Speaker 3: Dr. Elaine Cristina Mathias da Silva-Zacarin, UFSCar –
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Page 1: Biomarkers in Terrestrial Ecotoxicology: Stress Ecology in Bees

Biomarkers in Terrestrial Ecotoxicology: Stress Ecology in Bees

Chair: Dr. Fábio Camargo Abdalla, UFSCar – Sorocaba, [email protected]

“State of art of morphological biomarkers in ecotoxicology researche on bees”

Speaker 1: Caio Eduardo da C. Domingues - Post-graduation in Biotechnology & Environmental Monitoring

“Fat body, pericardial cells and hemocytes in association with the myogenic region: a model system to analysis the stress ecology in biomarkers of bees”

Speaker 2: Paulo José Balsamo – Post-graduation in Biotechnology & Environmental Monitoring

“An old subject, but a new challenge in ecotoxicology applied to the study of morphological biomarkers in bees: Hormesis”

Speaker 3: Dr. Elaine Cristina Mathias da Silva-Zacarin, UFSCar – Sorocaba, [email protected]

“Evaluation of cellular responses on bee organs exposed to xenobiotics by means of histological, histochemical and immuno-histochemical methods”

Page 2: Biomarkers in Terrestrial Ecotoxicology: Stress Ecology in Bees

Trusty?

Page 3: Biomarkers in Terrestrial Ecotoxicology: Stress Ecology in Bees

http://sos-bees.org/

Page 4: Biomarkers in Terrestrial Ecotoxicology: Stress Ecology in Bees

http://sos-bees.org/

Page 5: Biomarkers in Terrestrial Ecotoxicology: Stress Ecology in Bees

http://www.worldometers.info/ 14 August 2015.

Page 6: Biomarkers in Terrestrial Ecotoxicology: Stress Ecology in Bees

ALTERNATIVE ECONOMIC SYSTEM

EDUCATIONCHANGE THE COMSUMPTION’S “THOUGHT”

INCOME DISTRIBUTIONCHANGE THE WORLD AS YOU KNOW!

CHALENGE IN [ECO]TOXICOLOGY

PROSPECTIVE AND RETROSPECTIVE [ECO]TOXICOLOGY

LABELING XENOBIOTICSNEUTRALIZING/MODIFY XENOBIOTICS

Page 7: Biomarkers in Terrestrial Ecotoxicology: Stress Ecology in Bees

Do we want a labelling safety world or SAFETY WORLD?

Page 8: Biomarkers in Terrestrial Ecotoxicology: Stress Ecology in Bees

State of Art of Morphological Biomarkers in Ecotoxicology Researches on Bees

Methods for Assessing Exposure of Human and Non-Human Biota. Edited by R.G. Tardiffand B. Goldstein (f) SCOPE 1991. Published by John Wiley & Sons LId.

Do Classical Methods of Assessing Exposure in Bees are Effective for Ecotoxicology Diagnosis?

“While some relatively reliable methods have been developed for diagnosing some of these mechanisms (e.g., insect resistance to some pesticides), methods to measure precisely biological responses to exposure need to be improved.”

Page 9: Biomarkers in Terrestrial Ecotoxicology: Stress Ecology in Bees

State of Art of Morphological Biomarkers in Ecotoxicology Researches on Bees

“(1) Direct determination of xenobiotics at the level of cells and organs;(2) Qualitative and quantitative biochemical changes in some low- molecular and high-molecular substances; (3) Genetic changes at individual and population levels as indicators of exposure; and – (4) Effects of xenobiotics at the population level.”

Methods for Assessing Exposure of Human and Non-Human Biota. Edited by R.G. Tardiffand B. Goldstein (f) SCOPE 1991. Published by John Wiley & Sons LId.

Page 10: Biomarkers in Terrestrial Ecotoxicology: Stress Ecology in Bees

Do Classical Methods of Assessing Exposure in Bees are Effective for Ecotoxicology Diagnosis? Mortality rate Survival rate Behavioral Studies Specific Target Organ Studies

Could be not the answer for a Silent Damage!Could be not the answer for a Slow Damage Process!

Could be not the answer for Secondary Damage Effect!

BEES HAVE COMPENSATION SYSTEM!

Page 11: Biomarkers in Terrestrial Ecotoxicology: Stress Ecology in Bees

State of Art of Morphological Biomarkers in Ecotoxicology Researches on Bees

http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0132349

Page 12: Biomarkers in Terrestrial Ecotoxicology: Stress Ecology in Bees

Classical Methods

Morphology of Specific Target

Organs

Morphology of Secondary or Optimize

Stressor Organ

Page 13: Biomarkers in Terrestrial Ecotoxicology: Stress Ecology in Bees

HEART

THORAXHEAD ABDOMEN

Hepato-Nephrocitic SystemSinus Pericardium

Myogenic Region of the Dorsal Vessel

Hemolymph (+ xenobiotics)

Pericardial Cells

Fat Body (enocyte and trophcyte)

Hemocytes

Ostiole

Hemolymph

Page 14: Biomarkers in Terrestrial Ecotoxicology: Stress Ecology in Bees

50µm

Pericardial Cells

Trophocytes

Enocytes

Bombus morioCourtesy: Felipe Lissoni de Andrade Nogueira

Ostiole

Valve of the Myogenic Region

Valve of the Myogenic Region

Hemolymph (+ xenobiotics)

Page 15: Biomarkers in Terrestrial Ecotoxicology: Stress Ecology in Bees

Thank you all!

Special Thanks for Dr. Monica Jones Costa


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