8/14/2019 Bioplex ELISA Western Assays 2pg
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Call toll free at 1-800-4BIORAD (1-800-424-6723)
Doing ELISA’s?Cost/kit # Samples # of Targets Total # Cost per Sample Dynamic
of Assays sample/target Volume Range*
ELISA $500 80 1 80 $6.25 >1 ml 2 – 3,000pg/ml
$600 80 1 80 $7.50 >1 ml
Bio-Plex $475 80 1 80 $5.94 12.5 – 50µl 2– 32,000pg/ml
4-Plex $920 80 4 320 $2.88 12.5 – 50µl 2–32,000pg/ml
8-Plex $1,490 80 8 640 $2.33 12.5 – 50µl 2–32,000pg/ml
17-Plex $2,485 80 17 1360 $1.82 12.5 – 50µl 2–32,000pg/ml
27-Plex $3,670 80 27 2160 $1.70 12.5 – 50µl 2–32,000pg/ml
*Approximate range of standard curves. Experimental range dependent on assay target and sample matrix.
Ask me for a quote today.
Christina Anderson
1-800-876-3425 x8514
Cost Comparison Bio-Plex Phosphoprotein Assays vs. Western BlotsBio-Plex Assay Costs
Single Target Three Targets Five Targets
Assay $285.00 $855.00 $1,425.00
Reagent kit $235.00 $235.00 $235.00
Lysis kit $163.00 $163.00 $163.00
Total cost per assay plate $683.00 $1,253 $1,823.00 Estimated labor costs
Cost per sample (96-well assay) $7.11 $12.05 $18.98 Labor rate/hr $25.00
Cost per data point (96-well assay) $7.11 $4.35 $3.80 Hours hands on time 1.50 Total cost per data point pus labor $44.61 $41.85 $41.30 Total labor costs $37.50
One Target Three Targets Five TargetsWestern Blot Costs (midi-gel) Single Blot Three Blots Five Blots
Pre-cast gel (12 wells to run) $14.70 $44.10 $73.50
Protein standards $2.10 $6.30 $10.50
Transfer membrane and filter paper $9.50 $28.50 $47.50
Phosphoprotein primary antibody $40.95 $122.85 $204.75
Detection reagents $12.60 $37.80 $63.00
Buffers $3.15 $9.45 $15.75 Estimated labor costs
Total cost per blot $83.00 $249.00 $415.00 Labor rate/hr $25.00
Cost per data point (1 sample per data point) $7.55 $22.64 $37.73 Hours hands on time 3.00
Total cost per data point plus labor) $82.55 $246.64 $412.73 Total labor costs $75.00
Save Money, Time, and Sample with the Bio-Plex® Suspension Array System
8/14/2019 Bioplex ELISA Western Assays 2pg
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Rat
IL-1a •
IL-1b •
IL-2 •
IL-4 •
IL-6 •
IL-10 •
GM-CSF •
IFN-g •
TNF-a •
Bio-Plex®
Rat Cytokine, Chemo
and Growth Factor Assay
Bio-Plex Phosphoprotein and Total Target Assays Available Assays
CST antibodies exclusively developed
and validated for Bio-Plex phosphoprotein
and total target assays.
Assays Phosphoprotein Total
Akt (Ser473 ) ● ●
GSK-3α / β (Ser21 /Ser9 ) ●
p53 (Ser15 ) ● ●
p53 (Ser37 ) ● ●
p53 (Ser46 ) ● ●
Histone H3 (Ser10 ) ●
IκB-α (Ser32 /Ser36 ) ● ●
NF-κB p65 (Ser536 ) ●
STAT2 (Tyr689 ) ●
STAT3 (Ser727 ) ●
STAT3 (Tyr705 ) ●
STAT6 (Tyr641 ) ●
Tyk2 (Tyr1054 /Tyr1055 ) ●
IGF-IR (Tyr1131 ) ●
IR-β (Tyr1146 ) ●
IRS-1 (Ser636 /Ser639 ) ●
Akt Signaling
Cell Cycle/Checkpoint Control
Chromatin Regulation/Acetylation
Immunology/Inflammation
Glucose/Energy Metabolism
Assays Phosphoprotein Total
ATF-2 (Thr71 ) ● ●
c-Jun (Ser63 ) ● ●
ERK1 (Thr202 /Tyr204 ) ●
ERK2 (Thr185 /Tyr187 ) ● ●
ERK1/2 (Thr202 /Tyr204, Thr185 /Tyr187 ) ● ●
HSP27 (Ser78 ) ● ●
JNK (Thr183 /Tyr185 ) ● ●
MEK1 (Ser217 /Ser221 ) ● ●
p38 MAPK (Thr180 /Tyr182 ) ● ●
p90RSK (Thr359 /Ser363 ) ● ●
CREB (Ser133 ) ● ●
TrkA (Tyr490 ) ●
p70 S6 kinase (Thr421 /Ser424 ) ●
S6 ribosomal protein (Ser235 /Ser236 ) ●
Bcr-Abl (Tyr245 ) ● ●
c-Abl (Tyr245 ) ●
c-Abl (Tyr412 ) ●
EGFR (Tyr) ●
PDGF receptor-β (Tyr751 ) ●
Src (Tyr416 ) ●
MAP Kinase Signaling
Neuroscience
Translational Control
Tyrosine Kinases
Assays Human Mouse
IL-1α ●
IL-1β ● ●
IL-1ra ●
IL-2 ● ●
IL-2Rα
IL-3 ●
IL-4 ● ●
IL-5 ● ●
IL-6 ● ●
IL-7 ●
IL-8 ●
IL-9 ● ●
IL-10 ● ●
IL-12 (p40) ●
IL-12 (p70) ● ●
IL-13 ● ●
IL-15 ●
IL-16
IL-17 ● ●
IL-18**
Basic FGF ● CTACK
Eotaxin ● ●
G-CSF ● ●
GM-CSF ● ●
GRO-α
Assays Human Mouse
HGF
ICAM-1
IFN-α2
IFN- γ ● ●
IP-10 ●
KC ●
LIF
MCP-1 (MCAF) ● ●
MCP-3
M-CSF
MIF
MIG
MIP-1α ● ●
MIP-1β ● ●
MIP-2
β-NGF
PDGF-BB ●
RANTES ● ●
SCF
SCGF-β
SDF-1α TNF-α ● ●
TNF-β
TRAIL
VCAM-1
VEGF ●
Representative Working Assay Range
Analyte Serum Plasma
IL-1b 0.2–516 0.2–566
IL-2 2.7–4,599 4.5–4,570
IL-4 0.3–2,611 0.2–2,611
IL-5 3.7–1,354 5.0–4,355
IL-6 1.7–16,058 5.2–18,618
IL-10 0.7–8,591 1.1–11,850
IL-12 (p70) 0.6–3,993 1.7–3,994
IL-13 0.2–1,745 0.3–2,406
IFN-g 0.5–1,814 0.7–1,814
TNF-a 0.2–1,423 0.2–2,059
Bio-Plex Precision Pro™Human Cytokine Assay Panel
Bio-Plex Pro™ MagneticCytokine, Chemokine, and Growth Factor Assays
Available Assays Assay Range and Sensitivity
Targets Range Sensit
lgG1 3.00–7,950 ng/ml 2 ng/m
lgG2 20.00–6,000 ng/ml 20 ng/
lgG3 5.25–5,000 ng/ml 0.8 ng/
lgG4 0.06–810 ng/ml 0.08 ng
lgA 0.12–500 ng/ml 0.2 ng/
lgE 0.01–100 ng/ml 0.02 ng
lgM 0.42–1,500 ng/ml 0.2 ng/
Specifications for ImmunoglobuIsotyping Assays
The Bio-Plex suspension array system includes fuorescently labeled microspheres and instrumentation licensed to Bio-Rad Laboratories, Inc. by the Luminex Corporation.
A Multitude of Choices
Human Human Human
Acute Phase Angiogenesis Diabetes
a-2-macroglobulin Angiopoeitin-2 Adiponectin
CRP Follistatin Adipsin
Ferritin G-CSF C-peptide
Fibrinogen HGF Ghrelin
Haptoglobi n IL-8 GIP
Procalciton in Leptin GLP-1
SAA PDGF-BB Glucagon
SAP PECAM-1 IL-6
Tissue plasminogen VEGF Insulin
activator Leptin
PAI-1
Resistin
TNF-a
Visatin
Bio-Plex Pro DiseaseResearch Assays
Available Targets