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Dr Niramol Punbusayakul

Biosensor for Fruit &Vegetables Quality

Determination

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http://www.karstenfaehnrich.de/Biosensors/biosen

http://www.karstenfaehnrich.de/Biosensors/biosensors.htmhttp://www.karstenfaehnrich.de/Biosensors/biosensors.htm

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Biosensor & Its ImportanceAn analytical device that incorporates an immobilized

biological element which interacts with an analyte ofinterest in real time

Converting the biological response into an electricalsignal (readable)

ImportanceOffer analytical simplicitySelective, rapid (real-time) , specificity & sensitiveWide range of substances (target species)Mass producible, economic & disposableCan be operated in cpx matrices (not much sample

preparation needed)

User friendly & do not require much technical skills tooperateWith modern tech.: allow their integration into small

portable devices

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Biosensors ComponentsBiological recognition element: react

with the interested species

Transducer: convert the biological signal

to observable signalAcquisition device: amplify & provide

readable signal

http://www.dddmag.com/images/0409/HTS1_lrg.jpg

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How BioSensor Works?

Interest species react at the surface of the recognition element

Properties of the surface of the recognition element change

Transducer recognizes the change & converts that change into anobservable signal

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Recognition ElementsAntibodies & antibody fragmentsEnzyme

Cell membrane receptors

A living cellNucleic acid-based probes: DNA,

RNA/peptide nucleic acid

Aptamers

Chemically generated recognition surface:plastibodies (artificialantibodies/molecularly imprinted polymers)

http://www.ufz.de/index.php?en=

http://www.ufz.de/index.php?en=13023http://www.ufz.de/index.php?en=13023http://www.ufz.de/index.php?en=13023

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Classification of Biosensors:Biological recognition element

Biological recognitionelements

Biosensor types

Enzymes Enzyme electrode

Proteins

Antibodies Immunosensor

DNA DNA sensor

Organells

Microbial cells Microbial sensor

Plant & animal tissues

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Biosensors

Transducers

Biosensors

Signal determined

Electrochemical -Conductimetric-Potentiometric

-Votammetric

-Conductance change- Potential (E) when no current (I)flow (at equil)

-Apply different potential &determine current

Electrical Field effecttransistor

I flows along a semiconductor from asource gate to a drain, a small

change gate E large variation Ifrom source to drain

Optical SPR -Surface plasmon resonance

Thermal Calorimetry Heat exchange detected bythermisters & related to the rate of

reaction

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Ideal CharacteristicsBiosensorsHigh specific

biorecognitionelement

Reusable

High sensitivity

Cost effective

Rapid analysis time

Use of on-line/insitu measurement

Real-time resultsGood signal to noise

ratioRobustAbility to measure

samples in a high-throughput fashionif required

Possibility ofincorporation intoautomated roboticsystems

Fast turnaroundtime on anal sis

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Applications of Biosensors for

Fruit & Vegetable QualityDetermination

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Risk in Fruit & vegetableToxic materials:

herbicides &pesticidesIntentional added to

suppress growth ofplant & insectpopulationsMicrobial toxin:

mycotoxins:aflatoxin B1

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e o s or ua yanalysis

Method Advantages Disadvantages

- Visual monitoring- Analysis of soluble solidcontent- Monitoring of titratable

acidity

Fast after harvestingon location atminimal cost

Not sufficient for providingthe consumer satisfication oracceptable regulations

- HPLC & GC/MS AccurateHigh sensitive

-Time-consuming- Need expertise &expensive

- Using NIR, SEM Accurate - expensive, need expertise

- Detection of indicatormolecule (freshness:flavonoid), assessingproduct firmness, ,monitoring bitterness

easier - Less accurate (a lot of affecting factors)

- Non-destructive test:acoustic response - Many factors concerned

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ImmunosensorBiorecognition element:antibody

Interested species:

antigen

Transducer

Piezoelectric: QCM(Quartz crystalmicrobalance)Frequency changed with

weight

Reaction: antibody-antigen affinity

Observable signal: frequency

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Antioxidant Potential ofSome Less Common Fruit

Species

http://www.mdpi.com/1424-8220/8/12/7564/pdf

http://www.mdpi.com/1424-8220/8/12/7564/pdfhttp://www.mdpi.com/1424-8220/8/12/7564/pdf

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Optical Sensor

Fluorescent particle lab

Surface sorting of DNA tagged

nanoobjectshttp://www.lbb.ethz.ch/Research/Biosensors/Mart

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16/27http://www.nature.com/nrd/journal/v1/n7/fig_tab/nrd838

Optical Sensor

http://www.nature.com/nrd/journal/v1/n7/fig_tab/nrd838_F2.htmlhttp://www.nature.com/nrd/journal/v1/n7/fig_tab/nrd838_F2.html

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Strategy for SNP detection on thin-film biosensor chips

Zhong X et al. PNAS 2003;100:11559-11564

2003 by National Academy of Scienceshttp://www.pnas.org/content/100/20/11559/F1.expan

http://www.pnas.org/content/100/20/11559/F1.expansion.htmlhttp://www.pnas.org/content/100/20/11559/F1.expansion.html

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Antioxidants Ability to ScavengeFree Radicals Using Biosensors

https://www.landesbioscience.com/curie/chapte

https://www.landesbioscience.com/curie/chapter/4283/https://www.landesbioscience.com/curie/chapter/4283/

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Determination of PhenolicMetabolites

https://www.landesbioscience.com/curie/chapter

https://www.landesbioscience.com/curie/chapter/4403/https://www.landesbioscience.com/curie/chapter/4403/

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http://www.ejpau.media.pl/volume9/issue4/art-53.html

Enzymatic Biosensor

http://www.ejpau.media.pl/volume9/issue4/art-53.htmlhttp://www.ejpau.media.pl/volume9/issue4/art-53.html

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Optical Detector

http://www.biotek.com/resources/articles/cell-based-assay

http://www.business-sites.philips.com/sites/philipsbs/magnotech/press/pictur

http://www.biotek.com/resources/articles/cell-based-assays-reader.htmlhttp://www.business-sites.philips.com/sites/philipsbs/magnotech/press/pictures.pagehttp://www.business-sites.philips.com/sites/philipsbs/magnotech/press/pictures.pagehttp://www.biotek.com/resources/articles/cell-based-assays-reader.html

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DNA Micro Array Sensor

http://chem.chem.rochester.edu/~krauss/resear

http://chem.chem.rochester.edu/~krauss/research/karen.htmhttp://chem.chem.rochester.edu/~krauss/research/karen.htm

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Electrochemical Sensor

http://unit.aist.go.jp/rigb/gf-bmo/research_e

Monitor Enzyme

reactions Antigen-antibodyreactions

Nucleicacidhybridizations

http://unit.aist.go.jp/rigb/gf-bmo/research_e.htmlhttp://unit.aist.go.jp/rigb/gf-bmo/research_e.htmlhttp://unit.aist.go.jp/rigb/gf-bmo/research_e.html

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Interferometric biosensor

http://unit.aist.go.jp/rigb/gf-bmo/research_e

http://unit.aist.go.jp/rigb/gf-bmo/research_e.htmlhttp://unit.aist.go.jp/rigb/gf-bmo/research_e.html

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Peptide Hormone Sensor

http://unit.aist.go.jp/rigb/gf-bmo/research_e

http://unit.aist.go.jp/rigb/gf-bmo/research_e.htmlhttp://unit.aist.go.jp/rigb/gf-bmo/research_e.html

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http://www.mitre.org/news/digest/advanced_research/01_08/a_bio

http://www.mitre.org/news/digest/advanced_research/01_08/a_biosensor.htmlhttp://www.mitre.org/news/digest/advanced_research/01_08/a_biosensor.html

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Some good webs

http://www.biochip.umd.edu/payne/index.htm

http://www.biochip.umd.edu/payne/index.htmlhttp://www.biochip.umd.edu/payne/index.html