「 BioSystemforagricultureproducts」

EnhanceMicrobialGrowthAntimicrobialPropertiesinPlants

MicrobialCultureSolution「NutrientBrothforMicrobial

Growth」 •  Revolutionarypresent-dayProduct•  「NutrientBrothforMicrobialGrowth」Boostsimmensecellproliferationinmicrobialcount,providingplantswithageneroussupplyofnutrition(macronutrient).

•  Richinhighconcentrationsofaminoacidsofhighlyfunctionalmicrobialpolysaccharidetosoilorganicmatter.

•  Maybeappliedtoanyorganic/inorganicsolidfertilizer,liquidfertilizer,soilconditioner,pesticides,etc.,Asanadditiveaimedtocomplement,thatgreatlyintensifiestheresultingeffect.

ProductDefinition •  Providingthemainsourceofnutrientsformicroorganismslivingonthesurfaceoftheleavesorinsidethesoil,improvingtheirstateofnutritionaloptimization.Enhancefoliarnutrientuptakeandimprovesoilactivity,resultinginincreasedcropyieldandquality.

•  SoilMicrobescontainactivemicroorganismsandtheirrestingspores,afewexamples:bacteria(includingactinomycetes),fungi,algaeandothermicroorganismsofthemicrobialworld).Whenappliedtouseincropproduction,generatesdirectandindirectsuppliesofplantnutrientsorthepromotionoftheirnutrientuptake.

ProductFeatures

•  Themostbasicmicrobialnutrients-aminoacidsandnucleicaciddecompositionproducts,alongwithmicrobialfavoritesourceofsolublepolysaccharides,toassistinplantrootsabsorptionofnitrogenforalargenumberofrootgrowth.Solidifyrootsandincreasetheirabsorptioncapacity.Providingdecompositionenzymestotherootsandleaves.Enhanceplantrootgrowthandtheirwater,nutrientabsorptionability.Reducepestsanddiseases,improvethesurvivalrateofcroptransplantation,diseaseresistance,cold,drought,flowerandfruitresilience.

•  Earlyefficientfloweringandfruiting,optimizefruitqualityandyield,increasesuccessfulfruitingrates,fruitfullness,color,texture,sweetness,andnumbersareparticularlyemphasizedasimproved.

•  Reducetheneedforchemicaland/ororganicfertilizers,furtherreducingthecosts,andsoildegradationconcerns.

ProductComposition

•  NitrogenSource-•  1.Containapproximately50%:1.Asparticacid2.

Glutamicacid3.Serine4.Histidine5.TRO6.Glycine7.Arginine8.ILE9.Leucine10.Lysine11.Alanine12.Tyrosine13.Cysteine14.Phenylalanine15.Methionine16.Valericacid17.Threonine

•  2.NucleicAcid:Nucleotidedecompositionproducts,Purinebaseandpyrimidinebase,β-alanineandβ-aminoisobutyricacidetc.

•  CarbonSource-OrganicMatter•  1.Oligosaccharides+Polysaccharides:Mannan,Chitin,

Dextran

Soilrequiresmicroorganisms,our"ediblemicrobialculture"isthesourceoftreatmentfor

alltheirnutritionalneeds •  [ChemicalFertilizer–ExcessApplication]resultinginsalt

accumulation,reducednaturalsoilnutrients,causeofacidification,decreasedmicrobialcount.

•  [EnvironmentalPollution]Waterpollutionandairpollutionaretheculpritsofunbalancedsoilacidity,soilmicrobeshavebeenseverelyaffected.

•  [CropRotationMethods]Paddyfieldsanddrylandrotationcultivation,causesashiftintheratioofaerobicbacteriatoanaerobicbacteriainsoilmicrobes.

•  [Long-termAccumulation]Thelongtermcultivationofsingularcropchoiceshavelimitedsoilmicrobestoonlyaveryfewspecies,leadingtothereductionofnichediversity,andthebuildupofplanttoxicsubstances,allowingtheemergenceofpathogenicbacteria.

Plantsandthemicrobesintheirrootsmaintainaharmoniousmutually

beneficialrelationship •  1.Biologicalnitrogenfixation:Nitrogen-fixingbacteria

providethemainsourceofnitrogenforplants.•  2.Increaseorganicmatterdecompositionand

transformation:Promptingplantabsorptionofvariousnutrients

•  3.Growthstimulatingeffect:Rootmicrobescanproducemanyorganiccompoundsthataffectplantrootdevelopment

•  4.Antitoxiceffect:Rootmicrobialsecretionofantibioticsubstances,inhibitsthegrowthofotherharmfulmicroorganisms

ProductEffectonRootMicrobes Rootmicrobeconsumptionofnourishmentmediaboostsrapidreplicationofmicrobialcells.Nitrogenfixingbacteriaincreasesoilnitrogencontent.Decomposingmicrobescanbreakdownorganicresidues,asaplantnutritionsupply.PhosphorusDissolvingBacteriacanprocessinvalidphosphorusparticlesinthesoilintoplantusefulphosphoruscompoundsMicroorganismssecretepolysaccharides,thatallowssoilstructuralaggregationchangesforthebetterRoothairnumbersincreases,thelargertheabsorbingsurfacearea,thehighertheabsorptioncapacity,mycorrhizalandfungalgrowthofplantroots,withthemyceliumtoextendoutoftheroots,absorbsmorenutrients,especiallyintheuptakeofphosphorus,aswellasbeingdroughtresistant.Increasingdiseaseresistance,rapidmicrobialpropagationoftherootandleaf,secretionofdecompositionenzymes,destroyingthecellwallofpathogens,eliminatingharmfulpathogenicthreats,reducingbacterialinvasionoftherootring,achieving“BIOCONTROL"

AntibacterialmechanismA)+B)

A)Inducedbiochemicalplantacquiredresistance–

Secretionofglucanasechitinase

•  Plantsaredamagedbymechanical,chemicalorpathogenicmeans.Asaresponseproduceasequenceofbiochemicalreactions,thatcanrestoretheinjuredtissueeffectively.Higherplantspecieshaveavarietyofdefensivereactionstotheinvasionofpathogens:

•  1.Rapidsynthesisoflow-molecularantibacterialsubstances,suchasplantantibiotics,coagulationofcallosesugar-ß1-3glucanetc.tothickencellwall;

•  2.Largelysynthesizehydroxyproline-richglycoprotein,HPRGPthatstabilizesandtoughenscellwallfunction,aswellasglycinerichprotein,GRP;

•  3.Largelysynthesizecompoundstobreakdownthecellwallofinvadingbacteria,includingchitinase,glucanaseaswellaspathogenesis-relatedprotein;

•  4.Largelysynthesizeproteinaseinhibitor,PIthatcanpreventtheinfectionofmicrobesfromabsorbingnutrientsorinhibitinsectdigestivefunction

A)「NourishmentMedia」containingsmallmolecularcompoundsthatinducesplantcellsto

secretemoreß1-3glucanase

•  ActivatingthePlant’sImmuneSystem•  Innature,thesourceofß1-3glucanaseisveryextensive,theenzymeis

foundinbacteria,actinomycetes,algae,molluscsaswellasinlargequantitiesinhigherplantspecies.

•  ß1-3glucanaseandChitinaseiscorrelatedtightlyintheprocessofplantdiseaseresistance,thatexistinplantsandmicroorganisms

•  ß1-3glucanaseandChitinaseiscapableofhydrolyzingß1-3glucanandChitin,thesetwosubstancesarealsothemaincomponentsofthecellwallofmanypathogenicfungi.

•  Our「NourishmentMedia」stimulatesthegrowthofbothmicroorganismsandplantcells,inducingtheproductionofChitinaseandß1-3glucanase,andthebreakingdownofpathogenicfungicellwallintheβ-1,3andβ-1,6connectionthatbranchesthecompositionoftheconsistingpolysaccharide

B)ANewGenerationofNaturalFungicidesHighlyEfficient+non-toxic=Oligosaccharides+Polysaccharides

toactivatetheplant'snaturalimmunesystem

•  Spectrum-Applicabletorice,wheat,tomato,vegetablesandotherkindsofhigherplantspecies

•  Effect–underinvivoconditions,caninhibitavarietyofpathogenicmicroorganisminfections,againsttomatoleafmold,wheatpowderymildewandwatermelonwilthaveallshownpositivecontrollingeffects,andtheoverallperformanceofcropsinimproveddiseaseresistance,thatoffsetsandhinderdiseaseoccurrence.

•  Highefficiency-10ngoligosaccharides+polysaccharidesappliedto1gofplanttissueissufficienttoproduceenoughplantprotectionelements

•  Oligosaccharidesandpolysaccharides–Inducesplantprotectionelements,suchassynthesisofProteaseInhibitorsaswellastheaccumulationofpathogenesis-relatedPRproteins.Andcanincreasetheactivitiesofphenylalanineammonia-lyase,peroxidaseandotherdefenseenzymes.

•  Instantaneouseffect–following8hrleaftreatmentand24hrroottreatment,oligosaccharideswillbetransmittedandevenlydistributedthroughouttheentireplantbody,impressivelystrongdiffusionandconductionpropertiestothebaseandtopoftheplant.

HeavyMetalAnalysisTable TestSubject Method Result Remarks

Water-solubleboron CNS12968 notdetected MDL0.005％

WatersolubleCalciumOxide CNS13174 notdetected MDL0.05％

WatersolubleMagnesiumOxide

CNS12573 notdetected MDL0.05％

WatersolubleManganese CNS11463-2 notdetected MDL0.005％

Copper CNS13171 notdetected MDL15mg/kg

Zinc CNS12967 notdetected MDL15mg/kg

Arsenic CNS12954 notdetected MDL3.0mg/kg

Mercury MicrowaveDigestionClosedSolution

notdetected MDL0.05mg/kg

Cadmium CNS12955 notdetected MDL0.3mg/kg

Lead CNS13201 notdetected MDL3mg/kg

Nickel CNS13029 notdetected MDL3.0mg/kg

Chromium CNS13030 notdetected MDL3mg/kg

Conclusion PlantnourishmentBroth–ANutrientMediaforMicrobialGrowthforbiologicalfertilizer+biologicalpesticides.Improvecropproductivityandquality,increasefarmersprofits,reducetheuseofpesticides,safeguardingthewellbeingoffarmers,promotingalong-termsustainableagriculturalfutureforthethreeestablishingelementsofHarvest,Soil&Farmers.