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Biotech Tutorial
4-Biotechnology in the
Philippines
Evelyn Mae Tecson-Mendoza, PhD
Member, National Academy of Science and Technology Philippines
Professor Emeritus, Institute of Plant Breeding, Crop Science Cluster, College of Agriculture, University of the Philippines Los Baños
Copyright EMTecsonMendoza 2004-2013
Policies & MilestonesPolicies & Milestones
� 1979 National Institutes of
Biotechnology and Applied
Microbiology established
by Presidential Decree
under Pres. Ferdinand E.
Marcos.Marcos.
Under the University of
the Philippines Los Baños,
with then Chancellor Emil
Q. Javier as concurrent
first Director.
Institute of Plant Breeding (IPB), Institute of Plant Breeding (IPB), Crop Science InstituteCollege of AgricultureUniversity of the Philippines Los Baños
IPB is a national institute mandated to improve and develop new varieties of crops and conduct needed researches to support breeding objectives; lead institution for plant biotechnology as per Seed Development Act of 1992
Biotechnology in the PhilippinesBiotechnology in the Philippines
� 1979 National Institute of Biotechnology and
Applied Microbiology established by PD
under Pres. FE Marcos
� 1986-1992 Biotechnology—flagship
program under Pres. CC Aquino
� 1990 EO 430 creating National Committee
on Biosafety of the Philippines (NCBP)
� 1992-1998 Biotechnology---major S & T
program under Pres. FV Ramos; UP Biotech
institutes established by UP Pres. EQ Javier
Biotechnology in the PhilippinesBiotechnology in the Philippines
� 1997 Agriculture and Fisheries Modernization Act included provision for biotechnology
� 2000 Institutionalization of biotechnology in government programs, Pres. JE Estrada
� 2001 Policy statement on modern biotechnology � 2001 Policy statement on modern biotechnology by Pres. GM Arroyo
� 2002 Administrative Order (AO) No. 8 Rules and Regulations for the Importation and Release into the Environment of Plants and Plant Products Derived from the Use of Modern Biotechnology
Pres. G M Arroyo’s Policy
Statement on Biotechnology
“We shall promote the safe and responsible use of modern biotechnology and its products as one of biotechnology and its products as one of several means to achieve and sustain food security, equitable access to health services, sustainable and safe environment and industry development…”
July 2001
Agriculture
• Improved crop varieties, mostly by conventional breeding; GM crops for selected traits are emerging technologies
• Tissue culture raised planting materials and
Biotechnology in the
Philippines
• Tissue culture raised planting materials and cut flowers
• Biopesticides, local formulations of Bt, Biocon
• Biofertilizers-- Nitroplus, legume inoculant; Azospirillum; Mycorrhiza, Nitroplus, Cocogroe, etc
• Animal improvement, embryo transfer (ET)
Biotechnology in the
Philippines
Animal health
• Vaccines, a few developed locally; mostly
imported
• Diagnostics, mostly imported• Diagnostics, mostly imported
Foods and Feeds
• Diagnostics for important pathogens
Industrial and other biotech products
• Industrial enzymes, potential technologies
• Amino acid production, mostly imported
Biotechnology in the
Philippines
• Amino acid production, mostly imported
• Specialty biomolecular chemicals, monoglycerides, nata de coco--- need product development
• Recovery of value-added products from wastes, mostly imported; local potential technologies
orchids
Prof. Emerita de
Guzman
Prof. Helen Valmayor
makapunoorchids
1960s
1980s Dr. RC Barba led research on banana tissue culture at IPB
Plant Biotechnology-BasedBioindustries in the Philippines
Tools
Equipment*
Computer softwares Biochemicals
Process/Techniques*
Various DNA analyses*; Tissue
Institute of Plant Breeding, CA, UPLB1975
Services
Products
Various DNA analyses*; Tissue
culture (disease-free mother plants, for initial seedlings)**
Diagnostics*Tissue cultured seedlings*** Transgenic crops Genes Pharmaceuticals*indicates relative
usage
Commercialization of Plant
Tissue Culture Technologies
� Orchids
� Banana� Davao Musatech (Stanfilco), largest in the
world; produces 23 M seedlings per yearworld; produces 23 M seedlings per year
� Dolefil has capacity to produce 6 M per year
� Lapanday
� Secura Plant Genetics
� Makapuno
� Sugarcane
Development of transgenic crops with improved traits difficult to attain using traditional breeding techniques is ongoing at UPLB and at PhilRice.
The research in the International Rice Research Institute
based in Los Baños, Laguna is not described herein.
We are testing our transgenic papayas with delayed ripening trait at IPB
AG
Sajis
e
ACIAR-DOST-IPB-UPLB
At
Field testing of bacterial blight resistant transgenic rice (IR 72-Xa21
•Cloned the coat protein gene of the rice tungro spherical virus (RSTV) and transformed IR 72; tested transgenic rice in screenhouse http://www.philrice.gov.ph
Philippine Rice Research Institute (Philrice) Is Field Philippine Rice Research Institute (Philrice) Is Field Testing Golden Rice With IRRI.Testing Golden Rice With IRRI.
One of the first commercial Bt corn fields in South
Cotabato (July 2003). The lady farmer-owner said
that she would plant the Bt corn hybrid again
because of the higher yield and corn borer-
resistance. The latter meant non or less use of
pesticides.
Foreign biotech products tested Foreign biotech products tested and adopted in the Philippinesand adopted in the Philippines
For propagation
� Single transformation events
◦ MON 810
◦ Corn NK603
◦ Corn GA21
◦ Corn Bt11
For direct use as food, feed or for processing
As of 2013
Single traits/events
� corn 16 potato 2
� Soybean 7 sugarbeet 1
More than 700,000 ha of GM corn planted in PHL
◦ Corn Bt11
◦ MON 89034
� Stacked traits
◦ Corn MON810xNK603
◦ Bt11xGA21
� Canola 1 alfalfa 1
� Cotton 3 rice 1
Stacked traits
� corn 26
� cotton 3
genomicsgenomics
study of genomics provides
� knowledge and
� information
On genome composition, gene expression, On genome composition, gene expression,
and metabolic processes.
Can accelerate improvement of breeds of
crops and animals through better
fundamental insights and strategies using
genomics tools
Philippine Genome ProgramPhilippine Genome Program
� UP Diliman
◦ NIMBB
� UP Manila
◦ IMBB◦ IMBB
◦ National Institutes of Health (NIH) UP Manila
� Genome sequence & diagnostics for TB, malaria
� UP Los Baños
◦ IBS, CAS
◦ IPB-CSC, CA
Agriculture Program of the Agriculture Program of the Philippine Genome Center Philippine Genome Center
AIM: ENHANCE AGRICULTURAL AIM: ENHANCE AGRICULTURAL PRODUCTIVITY THROUGH GENOMICS
LEAD AGENCY: UPLB
Drs. RP Laude, EM Tecson-Mendoza, AC Laurena, MS Mendioro, ETM Ocampo, MGQ Diaz, RN Garcia.
Program Title: Marker Assisted Breeding
for High Fiber Quality and Virus Disease
Resistant Cultivars of Abaca
Use of Genomics Tools in Use of Genomics Tools in Crop ImprovementCrop Improvement
Project Title: Abaca Functional
Genomics: High Throughput Discovery of
Genes and Molecular Markers
Construction and Analysis of Saba Expressed Sequence Construction and Analysis of Saba Expressed Sequence Tags (EST) Library and Functional Analysis of Selected Tags (EST) Library and Functional Analysis of Selected TraitsTraits
� To construct cDNA/EST libraries of Saba
� To analyze the generated ESTs;� To analyze the generated ESTs;
� To identify novel and economically important banana Saba genes.
L. G
uec
o, IP
B
Field genebank of Wild Musa balbisiana collections at NPGRL
L. G
uec
o, IP
B
Evelyn Mae Evelyn Mae TecsonTecson--Mendoza, Project LeaderMendoza, Project LeaderStudy Leaders: Roberta N. Garcia, Rita P. Laude, Study Leaders: Roberta N. Garcia, Rita P. Laude, LaverneeLavernee GuecoGueco, , VisitacionVisitacionHuelgasHuelgas:Funded by : DA Biotech PIU:Funded by : DA Biotech PIU
Development of DNA Markers for Genetic Development of DNA Markers for Genetic Diversity Analysis, Fingerprinting and Sex Diversity Analysis, Fingerprinting and Sex Determination of Pili (Determination of Pili (Canarium ovatumCanarium ovatum) )
Dan
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Roberta N. Garcia, Evelyn Mae Tecson-Mendoza and Leon O. Namuco
IPB, CA UPLBFunded by the Department of Agriculture
BAR BIOTECH
Dan
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