Naturally ahead
Biotronic ® Top3Triple action for improved performance!
Per4izer – the breakthrough in animal growth performance
CHALLENGES IN ANIMAL PRODUCTIONCHALLENGES IN ANIMAL PRODUCTION
• high levels of productivity
• high-quality products
• bacterial challenge
Salmonella, E. coli, Campylobacter, Clostridia, Streptococcus…
Feed Water Environment GI tract
kill beneficial microflora
no effect on feed hygiene
drug-resistant bacteria
ANTIBIOTICS VS NATURAL PRODUCTS
reduction of common bacterial burden
improvement of digestibility
Natural products as potential alternative to antibiotics…
ACIDIFICATION
Feed hygiene and GIT health is improved…
Dietary supplementation by acidifiers:
reduces pH and buffer capacity
reduces pathogens in feed and GIT
stimulates growth promotion
ENHANCEMENT OF EFFICACY
The efficacy of organic acids might be improved…
combining them with phytochemicals!
Phytochemicals Organic acids
PHYTOCHEMICAL. DEFINITION
A term “phytochemical” refers to compounds produced by plants which have protective or preventive properties.
Phytochemicals are found in fruits, vegetables, beans, grains and other plants.
There are more than 1,000 phytochemicals:
Anti-oxidant: allyl sulfides, carotenoids, polyphenols
Antibacterial: allicin, cinnamaldehyde
Hormonal action: isoflavones (soy beans)
IMPROVING EFFICACY OF PRODUCTS
Individual products
Blends of organic acids & phytochemicals
Blend of organic acids, phytochemical & permeabilizers
PERMEABILIZERS. DEFINITION
Permeabilizers weaken the outer membrane (OM) of
Gram-negative bacteria facilitate other antimicrobials to enter
bacterial cells.
BACTERIA STRUCTURE
Outer membrane of Gram-negative bacteria contains lipopolysaccharide layer which makes bacteria resistant to:
• hydrophobic antibiotics,
• detergents,
• bile salts,
• digestive enzymes.
BIOTRONIC® PRODUCT LINE
Active ingredients:
Biomin® Per4izer
Phytochemical
Organic acids
Sequential Release Medium
Biotronic® Top3
ORGANIC ACIDS. DEFINITION. MODE
OF ACTION
pH reduction
dissociated
H+ R-
undissociated
Antimicrobial effect
RH
H+
H+
H+
ANTIMICROBIAL EFFECT OF ORGANIC
ACIDS
Bacteria is not ableto replicate
•pH reduction uses energy to restore pH
•Disruption of DNA and protein synthesis
•Disruption of membranes
pH 7Intestinal
pH 5-6
(Booth, 1985 – Stratford & Anslow, 1998 - Russel & Diez-Gonzales, 1998 - Roe et al., 1998 – Krebs et al., 1983 – Cherrington et al., 1983 – Freese et al., 1973)
CINNAMALDEHYDE
Cinnamaldehyde is the organic compound that gives cinnamon its flavor and odor. It occurs naturally in the bark of cinnamon trees and other species of the genus Cinnamomum.
Cinnamaldehyde plays an essential role in the celldivision reducing the proliferation of potentially harmful bacteria.
PROKARYOTIC CELL DIVISION
Nature Reviews. Molecular cell biology
Cinnamaldehyde
Inhibit FtsZ protein and GTPaseactivity, and assembly into Z-ring.
Bacteria does not divide!
FtsZ = filamentation temperature sensitive protein-Z GTPase = guanosine 5-triphosphate
Domadia et al., 2007
INNOVATION BIOMIN® PER4IZER
Biomin® Per4izer is the unique permeabilizing substance identified to enhance the antimicrobial effect of the organic acids andthe phytochemical against pathogenic bacteria.
1. Per4izer weakens gram-negative bacteria outer membrane
2. Per4izer facilitates entry of organic acids and phytochemical
3. Lethal injury of bacteria
Blend of organic acids
Biomin® Per4izer
Phytochemical
Sequential Release Medium
CARRIER – SEQUENTIAL RELEASE
MEDIUM
CARRIER
Vermiculite: phyllosilicate/sheet silicate clean to handle odorless mold resistant.
Vermiculite exfoliated expands in a free flowing powder which is able to absorb different substances and serve as carrier.
TRIAL RESULTS IN VIVO
BW vs NC +7%, vs PC +11%
ADWG vs NC +8%, vs PC +16%
FCR vs NC -3%, vs PC -1%
BW vs NC +7%,
ADWG vs NC +8%
FCR vs NC -3%
EFFECT OF BIOTRONIC® TOP3 ON
GROWTH PERFORMANCE IN WEANED PIGS
Negative control Biotronic® Top3 Δ, %
No. animals n = 30 n = 30
Initial body weight, kg 8.75 8.72 /
Final body weight, kg 35.62a 37.69b +6
Feed intake, g/animal 982 1028 +5
Daily weight gain, g 481a 517b +8
FCR 2.04 1.99 -3
Species : weaned piglets (days 28)Duration: 56 dNC = no growth promoterBiotronic® Top3 = 1 kg/t
a,b Means with different superscripts differ significantly; P<0.05
ROI = 11:1Economical benefit 2.5 €/pig
COMMERCIAL TRIAL, CRAILSHEIM, GERMANY
Trial designTreatments:1) Negative control (NC) – no AGP or NGP
2) Treatment – Biotronic® Top3 1 kg/t
90 piglets weaned at 28 days of age/group
Duration of the trial: 56 days
Parameters:
Growth performance (BW, ADWG, FCR)
COMMERCIAL TRIAL, CRAILSHEIM, GERMANY
Results
29.5
30.0
30.5
31.0
31.5
32.0
Control group Biotronic® Top3
group
Body weight (kg), 84 days
30.2
31.5
420
430
440
450
460
Control group Biotronic®
Top3 group
Average daily gain (g)
434
454
1.45
1.5
1.55
1.6
Control group Biotronic® Top3
group
Feed conversion ratio
1.55
1.51
ROI = 9.8:1
4.4%
4.6%
2.6%
SCIENTIFIC TRIAL, HUNAN UNI, CHINA
Trial designTreatments:1) Negative control (NC) – no AGP or NGP
2) Positive control (PC) – Colistin 100g/t + CTC 100g/t
3) Treatment (BTR Top3)– Biotronic® Top3 1 kg/t
Parameters:
Growth performance (BW, ADWG, FCR)
pH value in feed and GIT
Bacteria in GIT
Histology of jejunum
SCIENTIFIC TRIAL, HUNAN UNI, CHINA
Results on growth performanceNC PC BTR
Top3
Δ, %
NC vs BTR
Top3
Δ, %
PC vs BTR
Top3
No. animals n = 32 n = 32 n = 32
Initial BW d35, kg 10.0 10.9 10.7 / /
Final BW d91, kg 42.5a 43.2a 48.1b +13 +11
FI, g/animal/day 1092a 1112a 1269b +16 +14
DWG, g 580a 578ab 668b +15 +16
FCR 1.88 1.92 1.90 +1 -1
Mortality, no ofanimals
1 2 /
a,b Means with different superscripts differ significantly; P<0.05
SCIENTIFIC TRIAL, HUNAN UNI, CHINA
a,b Means with different superscripts differ significantly; P<0.05
Results on pH reduction in feed and GIT
NC PC BTR Top3
pH in feed 5.78 5.73 5.59 0.19
Buffer capacity in feed,
ml of HCl21.7 20.1 13.2 8.5
pH in stomach 4.28a 4.30a 3.67b
0.6
pH in doudenum 5.39 5.35 5.75
pH in jejunum 6.53 6.58 6.42
pH in ileum 6.97 7.02 6.89
pH in colon 6.28 6.52 6.51
SCIENTIFIC TRIAL, HUNAN UNI, CHINA
Results on microbes and morphology of GIT
NC PC BTR Top3
Ileum, Log cfu/g
E. coli 6.302a 5.970b 5.873b
Coliforms 8.277a 7.977b 8.151b
Salmonella typhimurium 8.220a 7.941b 8.053ab
Lactobacillus 6.295a 6.172a 6.515b
Bifidobacteria 6.891a 6.878a 7.130b
Histology of jejunum
Villi length, µm 418.33a 448.63ab 456.18b
Crypt depth,µm 192.37 188.77 187.15
a,b Means with different superscripts differ significantly; P<0.05
CONTRIBUTION TO FEED HYGIENE
Agar diffusion test
The effect of Biotronic® Top3 on the growth inhibition of mold and yeast was evaluated
RESULTS
Strains Inhibition after 96h
Aspergillus fumigatus1 xxx
Fusarium proliferatum x
Candida famata xx
Candida glabrata xx
Candida krusei xx
Fusarium graminearum xxx
Monascus ruber xxx
Penicillium roqueforti x
1Excellent inhibition after 24h
PELLETING TEST
Mixer: Bühler Paddelmixer: Mixing accuracy d =0.00001 Mixing time dry (without fat added): 120 sec* Mixing time in total: 300 sec
Pelletiser: Bühler Serial number 10231288 Type: DPAA 660.178 Die plate: diameter of the openings: 3.5 mm, length 55
mm
Sampling: Samples taken were cooled on plastic plates before
packaging Samples were stored at 4°C until further analysis
PELLETING TEST
Sampling:3 samples of the pre-mixtures in order to determine mixing accuracy
3 samples from the experimental mixture after the mixing process - without any additional treatment
recovery of active ingredients
After steam injection (and before the pelletising process) 3 samples were taken and cooled to room temperature before packaging indication of damaged caused by
the steam injection (hydro thermal stability)
3 samples of feed pellets were taken and cooled to room temperature before packaging indication of damage
caused by steam injection and subsequent pelletisingprocess (hydro thermal-mechanical stability)
RECOVERY OF BIOTRONIC® TOP3 (90°C)
0
20
40
60
80
100
120
Control Steam heated Pelleted
Flavouring agents
Organic acids
CHLORTETRACYCLINE
0
20
40
60
80
100
120
E. coli O55:K59(B5) E. coli O128:H2 Salmonella enteridits Salmonella typhimurium
In
hib
itio
n,
%
Chlortetracycline Chlortetracycline + Biotronic® Top3
CARBADOX
0
20
40
60
80
100
120
E. coli O55:K59(B5) E. coli O128:H2 Salmonella enteridits Salmonella typhimurium
In
hib
itio
n,
%
Carbadox Carbadox + Biotronic® Top3
COLISTIN
0
20
40
60
80
100
120
E. coli O55:K59(B5) E. coli O128:H2 Salmonella enteridits Salmonella typhimurium
In
hib
itio
n,
%
Colistin Colistin + Biotronic® Top3
CONCLUSION
Biotronic® Top3 has no adverse effectson antibiotics
Biotronic® Top3 has an additive inhibitoryeffect effect with some antibiotics on somebacterial strains
Usage of Biotronic® Top3 has no influenceon recommended dosage of antibiotics!
CONCLUSIONS
Dietary supplementation with Biotronic® Top3
Improved growth performance
Lowered pH and buffer capacity in feed
Had an acidification effect in stomach
Decreased pathogenic bacteria
Spared beneficial bacteria
Stimulated growth of villi
Contributed to feed hygiene
APPLICATION
Dose: 1 kg/t of feed for swine
Swine: Pre-starter, starter, fattening
Weaning diarrhea: for optimum results Biotronic® Top3+ a conventional acidifier or probiotics.
.