BioUltra Manual Operare

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Microbiological Safety CabinetBIOULTRA

USERS MANUAL

Telstar Industrial, S.L.Josep Tapiolas, 12008226 TERRASSA (Espaa)Tel. +34 937 36 16 00Fax: +34 937 85 93 [email protected]

BIOULTRA_I_ver0106US.doc


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SUMMARY

0.

SYMBOLS AND ABBREVIATIONS______________________________________ 3

1. GENERAL DATA___________________________________________________ 3

2. DESCRIPTION OF THE SAFETY CABINETS ______________________________ 42.1. working principle _____________________________________________ 42.2. Applications and principle diagram ________________________________ 42.3. Components description ________________________________________ 52.4. standard equipment ___________________________________________ 52.5. Certification _________________________________________________ 52.6. Technical features_____________________________________________ 5

3. INSTALLING INSTRUCTIONS ________________________________________ 63.1. Transport ___________________________________________________ 63.2. Cautions for first connection_____________________________________ 8

3.3.

Electrical connection and services_________________________________ 83.4. Cabinet ductwork _____________________________________________ 9

3.5. Certification or installation test___________________________________ 10

4. OPERATING INSTRUCTIONS_________________________________________ 114.1. Start-up ____________________________________________________ 114.2. Front panel positions(additional keyboard)__________________________ 114.3. General Working recommendations _______________________________ 12

4.3.1. Ergonomics ______________________________________________134.4. Definition of the work zone______________________________________ 164.5. Remote control case and menus__________________________________ 164.6. Disconnection ________________________________________________ 214.7. Prolongated stoppage__________________________________________ 214.8. Optional accessories ___________________________________________ 22

5. MANTEINANCE ___________________________________________________ 225.1. Maintenance and control table ___________________________________ 225.2. Absolute filters _______________________________________________ 235.3. Spare parts__________________________________________________ 24

6. CLEANING AND DISINFECTION ______________________________________ 246.1. Surface cleaning and disinfection. ________________________________ 246.2. Glass front cleaning and disinfection ______________________________ 246.3. Disinfection with formaldehyde___________________________________ 24

7. MAINTENANCE CHART _____________________________________________ 24

8. ELECTRIC DIAGRAMS ______________________________________________ 24

9. TESTS__________________________________________________________ 259.1.

Impulsion filters integrity Test ___________________________________ 25

9.2. Exhaust filters integrity Test_____________________________________ 259.3. Inhalation leak Test (only CYTOULTRA model) _______________________ 259.4. Downflow air velocity Test ______________________________________ 25

9.4.1. Set point alarm downflow air velocity Test______________________259.5. Inflow air velocity test _________________________________________ 25

9.5.1. Set point alarm inflow air velocity test _________________________259.6. Smoke test __________________________________________________ 259.7. Light test ___________________________________________________ 259.8. NOISE level test ______________________________________________ 25

10. POSSIBLE PROBLEMS AND SOLUTIONS ________________________________ 26

11. CERTIFICATES ___________________________________________________ 29

11.1.

CE _________________________________________________________ 2911.2. Guarantee___________________________________________________ 29


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0. SYMBOLS AND ABBREVIATIONSList of stickers used in the biological cabinets.

EARTH WIRE PROTECTED STICKER.

FUNCTIONAL EARTH WIRE STICKER.

BIOHAZARD STICKER.

CYTOTOXIC HAZARD STICKER.

CLASS II CABINET CERTIFIED ACCORDING TOEN-12469-2000 STICKER.

MACHINE CERTIFIED BY TV STICKER.

Abbreviations used in this manual

V Volts s, h Seconds, hours

A Amperios Hz Herzius

W, kW, W Wats, kilowatts, microwatts dB Decibel

mm, cm,m

Milimetres, centimetres,metres

C Degrees

kg Kilogram

1. GENERAL DATAOperating Instructions Manual for Microbiological Safety Cabinets Class II, BIOULTRASTANDARD, which ensure (applying correct techniques in laboratory) high level protection forthe operator, the environment and the sample.

BIOHAZARD

CYTOTOXIC

CERTIFIED ACCORDING


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WARNING

This Manual must be read and understood before its installation, maintenance and operation ofthe biological safety cabinet Class II. All the safety instructions given in the Manual must befollowed.Any use or application failing to comply with the recommendations expressed in the Manual maycause danger and loss of rights to manufacturing guarantee as well as freeing themanufacturer from all responsibility arising.

Any maintenance must be done by qualified staff according to the laboratory working correctconditions. In case these actions are not carefully carried out, as a result the safety cabinet canwork in abnormal conditions.

All maintenance actions on this equipment must be performed by a qualified technician who isfamiliar with the proper maintenance procedure required for this equipment.

MANUFACTURER : TELSTAR INDUSTRIAL, S.L.Jos Tapiolas, 12008226 TERRASSA (ESPAA)Telf (+34) 93 736 16 00Fax (+34) 93 785 93 42e-mail: [email protected]://www.etelstar.com

2. DESCRIPTION OF THE SAFETY CABINETS2.1. WORKING PRINCIPLECLASS II Biohazard Safety Cabinets are designed for microbiology work with the mostadvanced microprocessor control, ensuring a high level of protection for the operator,environment and sample.

Cabinet exterior in laminated, lacquered, oven-dried stainless steel of a functional designwith reduced external measurements and front access for changing filters.

Spacious internal chamber with automatic sliding front made from laminated safety glass.Segmented work surface and liquid collection tray in polished stainless steel.

Three-fan system: two for the laminar down flow and a third for exhaust, providing doublesecurity. In the case of a breakdown of one of the laminar down-flow fans, the other onecontinues to provide airflow to the laminar flow while the exhaust fan slightly increases itsvolume to ensure good depression in the work zone. In the event of both the laminar down-flow fans failing, the exhaust fan can increase its volume enough to provide the depression

needed.As can be seen in Annex A2, the air propelled by the laminar down-flow fans (1) is unloadedinto the chamber or plenum (3) and, via the Absolute HEPA filter (5), is filtered and entersthe laminar regime in the work zone (7), obtaining Class 100 according to US Fed. Std.209D. The exhaust air is expelled by the fan (2) via a second plenum (4) and Absolute HEPAfilter (6) to the outside (9).

System of recycled, sterile vertical laminar flow in depression of 70% and exhaust of 30% ofsterile air, approximately.

2.2. APPLICATIONS AND PRINCIPLE DIAGRAMThe MSC models are appropriate for handling biologically pathogenic samples or products (up

to level-3 risk micro organism), ensuring Class 10 protection of sterile air in the work zone,as well as a high level of protection for the operator and the environment. Principle diagramin Annex A1.


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WARNING

Under no circumstances, is possible handling in corrosive, inflammable or explosive gasambient.

It is recommended that extracted air should be expelled to the exterior.

Right works conditions are obtained only when the in and out blowing fans green LEDs areon (green colour).

2.3. COMPONENTS DESCRIPTION(See Annex A2)

2.4. STANDARD EQUIPMENT 6A - 1380W socket distributed in two inlets on either side of the work zone. Insulation

level IP44 IK07. Easy-to-work, microprocessor-controlled UV germicidal lamp with a timer function. Can

only be enabled from the Disinfection display (Section 4.5 point 4.2). Average disinfection intensity over the work zone of 200 W/cm2 with a 20%

depreciation after a working life of 9000 hours.

WARNING

In the event of using this accessory, you should consider the normal precautions in the useof these types of lamps. Their use is only suitably for previous external sterilisation ofmaterials introduced in the cabinet. That is why the lamp is a previous accessory to working

in the cabinet and it is essential to keep it turned off when the operator is in front of thetable or working on it. SECURIT glass provides protection from direct radiation but UV raysare reflected on polished metal parts and can seriously damage an operators eyes. That iswhy the glass front automatically slides into the closed position and the fans disconnectwhen the UV light is connected.

2.5. CERTIFICATIONQuality control and results certification of trials carried out in line with standards. Certificateon the inside of the cabinet.

2.6. TECHNICAL FEATURESCharacteristics Units BIOULTRA

External dimensions

(LxWxH) (mm)

1400 x 795 x 1430.

Internal dimensions LxWxH

(mm)

1190 x 565 x 650

Front opening height cm. 200

Weight Kg 200

Laminar flow velocity air /

Laminar flow

m/s -

m3/h

0,40 / 1064


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Front velocity air inlet /

Exhaust flow

m/s -

m3/h

0,55 / 540

Power supply KW 2,1 kW

Lighting Lux > 750

Noise dB (A) < 60

Vibration MmRMS

< 0,005

Tension V 230

Filters HEPA/ULPA H 14 Filtering system in the exhaust and the impulsion99,999% D.O.P.

Fans Three high-efficiency fans two in laminar flow andthe other in the extraction. Flows measured by

thermoanemometric probes.

Microbiological Safety Cabinet BioUltra, 50Hz, 230V

DISSOCIATED DECLARED VALUES OF NOISE EMISSIONStandard(s) to which conformity is declared ISO 4871

Vdownflow= 0,4m/sVinlet= 0.6 m/s

LpA (dB) 55.3

KpA (dB) 2

Certain values according to acoustic test given in standard EN 12469:2000 using standard NormaISO 11201

3.INSTALLING INSTRUCTIONSFor a correct start-up procedure of the MSC, observe the following step-by-step instructions.

3.1. TRANSPORTThe cabinet is supplied in an individual package and in the suitable way to prevent it frombeing damaged


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WARNING

For any handling during assembly or dismantling of the cabinet, appropriate safetymeasures, such as wearing safety gloves or shoes, should be taken.

a) Cabinets should be placed away from entrances, corridors, ventilation supply points, etc.,which might affect airflow in the work zone.

b) Try to ensure the cabinet is set perfectly on the table or workbench in order to preventvibrations.

c) To stop the work zone from overheating, we recommend the room temperature shouldfall within the comfort margins for working.

The optimal comfort margins are:

- Temperature: 17-28C.

- Relative humidity: less than 90%.

If either of these conditions is not met, optimum cabinet performance cannot beguaranteed.

d) When necessary, air vents in the room should be modified or speed of air entry reducedso that air is not aimed directly at the cabinet, as this may produce turbulence.

Side air vents are not appropriate for rooms where cabinets are installed.

e) Given that the ceiling temperature affects air distribution, sites should be selected with

enough space. We recommend leaving a 500mm space between the exhaust and theceiling.

f) The site of the cabinet will directly influence the noise level. This level will riseconsiderably in small spaces with very reflective surfaces because of the proximity of thecabinets to the walls, and where fan speed has risen to compensate for air currents inthe room or the resistance of clogged filters.

g) The site should allow disinfection gases with formaldehyde (Annex A5.2) to be expelledinto the atmosphere.

h) Correct cabinet performance should be tested before putting it into operation if changeshave been made to the room or to the air intake system which may considerably alterthe airflow characteristics in the room.

i) We recommend positioning the cabinet in such a way that it makes it hard to accidentallydisconnect it, e.g., next to a wall or another piece of equipment.


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3.2. CAUTIONS FOR FIRST CONNECTION Before connecting it to the mains supply, give the cabinet a clean to eliminate any dust

particles that may have accumulated during transportation. Follow the instructions on

cleaning and disinfection.

Before using it for the first time, leave the cabinet running for around six hours to purgethe filters. New filters give off a characteristic smell which tends to disappear in a shortperiod of time.

WARNING

The cabinet should work at the voltage shown on the characteristics plate. It should beconnected to a suitable socket base (minimum 16A) with an earthed plug. The cable sectionrecommended for the base is 2.5 mm.

3.3. ELECTRICAL CONNECTION AND SERVICESThe electrical characteristics of the cabinet are as follows:

Electrical class: Class I. Insulation level: IP-20.

The cabinet is supplied with single-phase electro-fans and a connection lead with a Europeanplug (other connections available as optional accessories). The on/off button is in theremote-control case (Annex A3 pos. 1).

The power point in the wall should be sited in a perimeter around the cabinet of:

1.25 m from the centre of the cabinet to the sides 2.5 m from the base of the cabinet upwards

On the top right-hand side of the cabinet and identified by name are:

Voltage input (1) Checking valve for D.O.P. control (2)

Liberating screws and opening electrical drawer lid (3) you can accede to gas sockets (4)and vacuum ones (5).

EARTHWIRE


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WARNING

The cabinet can only be activated from the button in the remote-control case.

In the event of a power failure the cabinet will stop, showing the Power Failure alarm andautomatically closing the front.

Remove hands from the work zone when power failure occurs.

The installation to which the cabinet is connected should meet the safety requirements inforce in each country.

Exhaust air should be evacuated outside the lab if working with volatile substances notneutralised by absolute filters.

During normal work in these safety cabinets and because of a basic principle of recirculationand depression in the work zone, no perforated surface in the work zone should be coveredwith paper or sanitary fabric.

3.4. CABINET DUCTWORKIf a duct is installed to evacuate the sterile exhaust air outside, it should not be more than 4metres long. If the duct has to be longer, it should have a larger diameter or asupplementary exhaust fan should be installed at the end of the run. We recommendconsulting the distributor or manufacturer.

In the event of installing an additional fan, a potentiometer should be connected to regulateits speed in order to synchronise the exhaust airflow from the duct with the exhaust airflowfrom the cabinet. We also recommend interconnecting the exhaust fan and the cabinet on/offbutton so that it turns on and off together with the cabinet.


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WARNING

In the event of having to install an additional fan, we recommend positioning it at the end ofthe run outside the lab facilities so that the exhaust duct is always under negative pressure.

The outlet of the exhaust duct to outside should be duly protected against the entry of air

currents, insects and small animals.

3.5. CERTIFICATION OR INSTALLATION TESTAccording to standard EN 12469 and in order to ensure correct cabinet operation, TELSTARrecommends that after the installation and connection of the cabinet, the following testshould be done (before the equipment is used for the first time):

- 9.1. Impulsion filters integrity Test.

- 9.2. Exhaust filters integrity Test.

- 9.3. Test for leaks in the aspiration filter (CytoUltra model only).

- 9.4. Downflow air velocity.

- 9.4.1. Set Point alarm downflow air velocity Test.

- 9.5. Inflow air velocity Test.

- 9.5.1. Set Point alarm inflow air velocity Test.

- 9.6. Smoke test.

- 9.7. Light Test.

- 9.8. Noise Level Test


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Telstar, its representatives and distributors put a qualified technical team at your dispositionthat uses control equipment with maximum health protection for carrying out this installationtest under full safety guarantees.

4. OPERATING INSTRUCTIONS4.1. START-UPOnce the above points have been controlled, start up the MSC by pressing the On/Off button(Annex A3, pos. 1). After entering the password correctly (or immediately if this functionhas been disabled), the fans will automatically connect and the front will move into the workposition (Annex 1.2).

Ensure that the aspiration zone and air exhaust outlet are free (Annex A2 pos. 8 and 9).

The electronic system of regulation by microprocessor via 2 thermoanemometer probes,laminar down flow and exhaust airflow, needs a few seconds to stabilise during connection inorder to begin to work normally.

The cabinet can only be used by qualified staff who meet the correct work techniques in thelaboratory. If these techniques are not respected, faulty working of the equipment couldresult.

4.2. FRONT PANEL POSITIONS. ADDITIONAL KEYBOARDThe front can be moved independently of the main control display by using the additionalkeyboard located on the right-hand side of the work zone.

The additional keyboard works the same as the positioning keys on the front of the maincontrol (Section 4.5, point 3).


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- First press: completely opens the front.

Second press: stops the movement of the front.

- First press: moves the front into the work position.


- First press: completely closes the front.


The additional keyboard is enabled whenever the cabinet is connected to the mains supply(even when the cabinet is turned off) and is not blocked (Section 4.5, point 4.2). This makesit possible to move the front for cleaning and maintenance work without having to know theuser password.

4.3. GENERAL WORKING RECOMMENDATIONSa) We recommend starting up the cabinet around 10 minutes before you begin to work.

This will ensure particles are swept from the work zone and from material introduced.

b) Wash your arms, hands and nails well, using an anti-bacterial soap, before and afterwork. Staff should avoid touching their mouths and eyes.

c) We recommend wearing lab coats with adjusted cuffs and, in special works, protectiongloves (or sleeve cuffs). Both the coat and cuffs should be made from a fabric that

minimises the emission of fibres and particles.

d) The work zone inside the cabinet should not be used to store lab equipment. This canproduce an accumulation of dust that is unnecessary and hazardous for working understerile conditions. The fewest pieces of equipment possible should be positioned in orderto work correctly and with the minimum number of interruptions for the user (insertingand removing arms from the work zone).

e) Although the work zone is under a clean and sterile laminar down flow, the area aroundthe cabinet might be contaminated. You should know the sources of contamination toprevent them when inserting arms, material, etc.

f) All the material needed for the work should be free of particles and carefully cleanedbefore it is entered.

g) Materials such as paper, wood, cardboard, pencils, rubbers, etc. should not be entered inthe work zone as they shed a great many particles.

h) In the event of using pipettes, they should be of a mechanical aspiration type and youshould never pipette by blowing with your mouth because it is easy to inhale aerosolscaused buy suction.

i) When platinum handles are used, it is advisable to use electric incinerators, preferablysingle-use ones.

j) If the work requires the use of a gas flame from a Bunsen burner or similar, werecommend using a type where the flame is maintained by holding down a button. Please

note that use of a constant flame burner creates significant turbulence. Bear in mind thattoo large a flame could burn the absolute filters.


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k) Shadows and turbulence provoked by objects, equipment and materials sited in the workzone should be studied before work commences in order to evaluate their possibleeffects. Bear in mind that the laminarity of the airflow does not recover until a distanceof 2.5 times the diameter of the object that causes the obstruction.

l) When using bottles and tubes, they should preferably have a screw top instead of cottonstoppers which easily shed a large number of particles.

m) When the work is finished, all disposable products (handles, Petri dishes, etc.) as well asmeans of cultivation, samples, tubes, bottles, etc. should be removed from the cabinet inimpermeable bags suitable for sterilisation if necessary.

n) When working in the cabinet and during cleaning operations, it is important to preventdamage to the HEPA filters occasioned by knocking them, projecting liquids or splashingthem, etc.

o) All equipment connected to the base inside the cabinet should be fitted with an earth

wire and meet all the electrical specifications.p) People traffic generates air movements that may cause unexpected loss to the

containment barrier and, in the case of Class II cabinets, a reduced level of productprotection. Warning signs and other means should be used to prevent traffic duringcritical operations.

Cabinet sites can be divided to reduce the effects of air movement produced by othercabinets and people traffic.

q) Suddenly opening and closing doors may disturb airflows in the cabinet and should beavoided when cabinets are being used.

WARNING

1.- The UV light system should only be used when the front is closed.

2.- Never connect equipment whose consumption exceeds that indicated on the earth wireinside the cabinet.

4.3.1. ERGONOMICSMicrobiological security cabinet BioUltra and its accessories have been designed followingrecommendations and specifications of the standard EN ISO 14738:1999.

Users should consider their posture when working with the cabinet, for their own safety.

Below are examples of four postures NOTrecommended and one that ISrecommended.


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Fig. 1 X Fig. 2X

Fig. 3X Fig. 4X


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Adjusting the seat and adopting a correct posture enables the worker to work safely and toprevent any possible physical damage (sight, neck, back, etc.)

Fig. 5

Also, as protection for both the product and the user, it is necessary that the cabinet is sitedin a place without air currents, which may cause turbulence in the laminar down flow.

Fig. 6 X


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4.4. DEFINITION OF THE WORK ZONETo prevent creating turbulence in the laminar down flow and to protect the user and product,it is necessary to establish a work zone inside the cabinet.

Do not work in any area with perforations and do not cover them up.

Work 10 to 15 cm from the edges of the cabinet.

Fig. 7

4.5. REMOTE CONTROL CASE AND MENUS.The following elements are found in the remote-control case:

A. On/Off Button (Annex 3 pos. 1): Pressing when the cabinet is turned off:

Accesses the Password Request display. After entering the correct password (Section4.5 point 1), access the Main Operation display. If you have disabled the passwordrequest (Section 4.5 point 5) it will go straight to the Main Operation display.

Pressing when the cabinet is turned on:Accesses the Password Request display. After entering the correct password (Section4.5 point 1), the cabinet will switch off. If you have disabled the password request(Section 4.5 point 5) it will switch off straight away.

B. 100% Exhaust Button (Annex 3 pos. 2):The exhaust fan raises its airflow to the limit to ensure maximum user protection.


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C. Electrically operated gas valve connected LED (Annex 3 pos. 3):

Indicates that the electrically operated gas valve is connected, enabling gas to pass tothe tap of the work zone.

D. Plug enabled LED ( Annex 3 pos. 4):Indicates that the plugs in the work zone are enabled, so that current can be supplied toany electrical device connected to them.

E. Luminaire/UV lamp enabled two-colour LED (Annex 3 pos. 5):Indicates through the use of two different colours when the luminaire or UV lamp is

connected.

F. Fans connected LED (Annex 3 pos. 6):Indicates that the automatic airflow regulation system of the impulsion/exhaust fans isconnected and that they are working.

G. Backlit graphic display (Annex 3 pos. 7):Display that shows alarm messages, operating graphs, the value of the function buttonsand all the information required in cabinet-user communication.

H.

Function buttons (Annex 3 pos. 8):

Buttons whose function varies according to the display at which the user is situated.

I. Menu button (Annex 3 pos. 9):Enables the user to move through the different system displays.

Below is an explanation of the different system displays:

1. Password RequestDisplay.The four function buttons are enabled for password entry:

- F1 Increases the value of the selected field (_)

- F2 Confirms the value elected in a field and moves the selection to the nextfield. After pressing for the final time, if the password is correct, it accessesthe Main Operationdisplay.

- F3 Reduces the value of the selected field (_)

- F4 Provides access to the Language Selection display. Only appears after thefirst connection to the mains supply. If you want to change language againyou will have to disconnect and reconnect the cabinet to the mains.


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- F4 Provides access to the Cleaning display. Appears after the second and

subsequent connections.

1.1Language SelectionDisplay.The different languages available are assigned to a function button:

- F1 Spanish.

- F2 English.

- F3 German.

- F4 French.

Once you have selected the language, use the MENU button (Annex 3 pos. 9) to accessthePassword Requestdisplay.

1.2CleaningDisplay.This display enables you to access the cabinet to clean it without requiring the presenceof authorised staff:

- F1 First press: completely opens the front. Second press: stops themovement of the front. (The same for the additional keyboard).

- F2 Emergency stop.

- F3 First press: completely closes the front and stops the fans. Second press:stops the movement of the front. (The same for the additional keyboard).

- F4 Exit the Cleaningscreen and turn off the cabinet. The front must be in theclosed position to exit.

As well as the F4 function key it is possible to exit the Cleaning screen and turn the

cabinet off using the On/Off button (Annex 3 pos. 1). Again, the front must beclosed.

2. Main OperationDisplay.The most relevant data on how to operate the cabinet are shown on a graph. These dataare:

- Entry speed in the front aperture. Shows in m/s the value of the velocity of theprotection barrier for the user.

- Temperature inside the cabinet. Shows the temperature in C.- Laminar down flow velocity. Shows the value of the velocity in m/s of the laminar down

flow in the work zone.- Clogging of the impulsion filter. Filter clogging is shown in a numerical percentage andalso graphically.


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- Clogging of the exhaust filter. Filter clogging is shown in a numerical percentage andalso graphically.

- Exhaust airflow. The airflow is shown in m3/h.

The function buttons acquire the following functions:

- F1 Enables/disables the impulsion and exhaust fans.

- F2 Enables/disables the lighting.

- F3 Enables/disables the plugs in the work zone.

- F4 Enables/disables the gas tap in the work zone.

When the fans start up, the system needs a few seconds to stabilise. During this time, itis possible that an alarm may sound because of insufficient flow. To turn the acoustic

alarm off, the F4 button changes to . This function is only available at the start offan operation.

Press the MENU button (Annex 3 pos. 9) to access the Front Positioningdisplay.

3. Front PositioningDisplay.Enables the user to position the front as he or she likes:

- F1 First press: completely opens the front.


- F2 First press: moves the front into the work position.


- F3 First press: completely closes the front and stops the fans.


- F4 Emergency stop.

Press the MENU button (Annex 3 pos. 9) to access the Utilitiesdisplay.

4. Utilitiesdisplay.Provides the user with information on use time of the cabinet, fans, UV lamp and filters,as well as the last time the filters were changed. The function keys provide access to theMaintenance Serviceand Disinfectiondisplay:

- F1 Provides access to the Maintenance Servicedisplay.

- F2 Provides access to theDisinfectiondisplay.

- F3 Provides access to the Technical Service menu (access restricted tounauthorised staff) via thePassword Requestdisplay.


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- F4 No function.

Press the MENU button (Annex 3 pos. 9) to access the Password Configurationdisplay.4.1Maintenance ServiceDisplay.

This display shows the date of the last revision and provides the user with access to thefollowing functions:

- F1 Records the last revision done.

- F2 Shows a record of the history of revisions done.

- F3 Provides maintenance recommendations.

- F4 Alarms and principle failures.

Press the MENU button (Annex 3 pos. 9) to access the Utilities display.

4.2DisinfectionDisplay.Access the disinfection menu:

- F1 Moves the front to the closed position (Annex 1.2), disconnects the fans andconnects the UV light for the time determined by the timer.

- F2 UV Timer. Put the timer to zero to enable uninterrupted operation of the UV

lamp.

- F3 Provides access to the disinfection with formaldehyde displays which guidethe user through the different steps needed for correct disinfection.

- F4 Exit the Disinfectiondisplay and access the Utilitiesdisplay.

5. Password ConfigurationDisplay.Enables the user to configure the options regarding password use:

- F1 Blocks the cabinet to stop use by unauthorised staff.

- F2 Enables/disables the password request to Connect/Disconnect the cabinet viathePassword Request display.

- F3 Enables the user to change the access password via thePassword Requestdisplay.

- F4 If you forget the password, you can use this function to obtain a code. Whenyou give this code to the manufacturers after-sales service, the previouspassword will be recovered.

Press the MENU button (Annex 3 pos. 9) to access the Main Operationdisplay.


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J. Microprocessor:

The microprocessor maintains total control over the exhaust flow and impulsion flow via thethermoanemometer probe in the exhaust and the thermoanemometer probe in the work

zone. Each probe controls the corresponding fan independently (exhaust and laminar downflow) which means double security in the use of the cabinet:

The microprocessor consists of:

Backlit graphic display.

Alarms reflected on the microprocessor display:

No. ALARM SHOWN ON THE DISPLAY

1 Excessive laminar down flow (>10%) HIGH LAMINAR DOWN FLOW

2 Insufficient laminar down flow (


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4.7. PROLONGED STOPPAGEIn the event of a prolonged stoppage, try to prevent environmental dust from entering byturning the cabinet off with the front in the closed position (Annex A1.2) and covering upthe exhaust outflow at the top of the cabinet (Annex A2 pos. 9). When you turn it on again,it should work like the first time it was turned on. See Sections 3.3 and 4.1.

4.8. OPTIONAL ACCESSORIES Support table (Annex A4.1) Active Carbon Filter (Annex A4.2) Non-return exhaust valve (Annex A4.3)

WARNING

Gas is a toxic and inflammable substance which in the event of an accident can cause seriousdamage to the user and the cabinet.

The presence of a flame within the cabinet creates turbulence which affects its control andproper functioning, which is why we recommend you do NOT use burners unless strictlyessential and if the user is prepared to assume this responsibility.

Gas connection should be done in line with the safety standards of each country, region andcompany.

For these reasons, the electrically controlled valve that enables gas entry can only beactivated when the fans are working and will automatically disconnect when the alarmindicating a lack of impulsion flow goes off.

5. MANTEINANCEWARNING

All repair and maintenance work in the cabinet should be done by qualified technical staffwith knowledge about the maintenance procedures required by the cabinet.

Before starting any maintenance work or dismantling the cabinet, the area should be wipeddown with formaldehyde (Annex A5.2). Once this has been done, disconnect the cabinetfrom the mains.

In general, a regular control of the proper working of all cabinet components should be doneonce a year.

5.1. MAINTENANCE AND CONTROL TABLEThe frequencies determined for each operation, and in particular with regard to changingfilters, which appear on the Maintenance Table below are indicative values recommended fornormal cabinet service. Very severe work conditions may reduce the time intervals shown onthe Table.


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Maintenance Table

TEST REGULARITY

Impulsin filters integrity Test (AnnexA9.1).

Minimum once a year or after filter changes

Exhaust filters integrity Test (Anexo A9.2). Minimum once a year or after filter changes

Inhalation filter leak Test (only CytoUltramodel) (Annex A9.3).


Downflow air velocity Test (AnnexA9.4). During cabinet start-up

Set Point alarm downflow air velocity Test(Anexo A9.4.1).


Inflow air velocity Test (Annex A9.5). During cabinet start-up

Set Point alarm inflow air velocity Test

(Annex A9.5.1).


Smoke Test (Anexo A9.6) During cabinet start-up

Light Test (Anexo A9.7) During cabinet start-up

Noise level Test (Anexo A9.8) During cabinet start-up

5.2. ABSOLUTE FILTERSThe average life of HEPA filters in these cabinets is 4000/5000 hours.

After this time, the propelled airflow falls because of the increased loss of load of the filter asit is a mechanical filtering, although this does not mean that filtering efficacy is reduced.When it reaches the minimum flows valued by the probes, the microprocessor activatesvisual and acoustic alarms.

PRECAUTION

You cannot replace absolute filters or directly handle fans in MSCs without having first wipedthe area down with formaldehyde. Opening the cabinet without taking the right precautionscan endanger your health. We recommend it is done by staff especially prepared for thiswork.

When using formaldehyde it is necessary to follow the laws in force in each country.

To ensure correct installation and adjustment of the filter, as well as ensuring there are noleaks, filters should be replaced by specialised technical staff equipped with thecorresponding control instruments: anemometer, leak detector, aerosol generator, particlecounter, etc.

Clogged absolute filters left over from changes in safety cabinets should be destroyed. Thisprocess is always up the user. Our technical service will never take care of possiblycontaminated parts.

Optionally, our service offers special packaging for the user to transfer filters for theirsterilisation, incineration or destruction.


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5.3. SPARE PARTS

No. Ref. Description

1. 31437 Laminar down flow/exhaust fan

2. 11245 HEPA Filter 1220 x 610 x 69

3. 11246 HEPA Filter 610 x 610 x 69

4. 24477 Temperature probe

5. 15631 Velocity probe

6. 32073 36W germicidal lamp

7. 27044 Controller card

6. CLEANING AND DISINFECTION6.1. SURFACE CLEANING AND DISSINFECTION.

See Annexe A5.1

6.2. GLASS FRONT CLEANING AND DISINFECTION.

See Annexe A5.2

6.3. DISINFECTION WITH FORMALDEHYDETotal decontamination of the cabinet should be done using sterilising gas emission, whichensures penetration in sealed and inaccessible areas of the Cabinet. It also guarantees thesterilisation of the work zone, fan, plenum, HEPA filters and evacuation ducts.

Decontamination of the whole of the cabinet, including filters, fan unit and work surfaces, isusually done by fumigation with formaldehyde vapour. Alternatives may be used, e.g.,hydrogen peroxide. However, this may not be suitable in certain cases, such as when it is

necessary to follow specific regulations.See Annex A5.3 for the decontamination procedure.

7. MAINTENANCE CHARTSee Annex A6

8. ELECTRIC DIAGRAMSSee Annexes A7and A8


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9. TESTS9.1. IMPULSION FILTERS INTEGRY TESTSee Annex A9.1.

9.2. EXHAUST FILTERS INTEGRY TESTSee Annex A9.2.

9.3. INHALATION FILTER LEAK TEST (ONLY CYTOULTRA MODEL)See Annexe A9.3.

9.4.

DOWNFLOW AIR VELOCITY TEST

See Annex A9.4.

9.4.1. SET POINT ALARM DOWNFLOW AIR VELOCITY TESTSee Annex A9.4.1.

9.5. INFLOW AIR VELOCITY TESTSee Annex A9.5.

9.5.1. SET POINT ALARM INFLOW AIR VELOCITY TESTSee Annex A9.5.1.

9.6. SMOKE TESTSee Annex A9.6.

9.7. LIGHT TESTSee Annex A9.7.

9.8. NOISE LEVEL TESTSee Annex A9.8.


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10. POSSIBLE PROBLEMS AND SOLUTIONS

No. Type oferror

Causes of error Correction of error

Exhaust partially covered.Visually check the exhaust to eliminateany type of obstruction.

Front perforation in thework zone totally/partiallycovered.

Visually check the perforation andeliminate any type of obstruction.

Distance between cabinetexhaust and lab ceiling verysmall.

Increase space between cabinet exhaustand the ceiling. In some cases it is

possible to modify or eliminate the falseceiling.

Install a support table that is lower. Thismeans the work zone will also have to belowered.

Duct the cabinet (Section 3.5).

Clogged exhaust filter.

Check filter clogging with the cabinet innormal operation (Section 4.5 point 2).

Check the date of the last filter changeand the hours it has been operating

(Section 4.5 point 4).Notify the technical service to change thefilter.

Probe not working properly.

Check the probe position is correct.

Check that the exhaust box is positionedwith the perforation facing upwards.

1

Insufficientexhaust

flow

Alarm No. 3(see Section4.5 point J)

Cabinet ducted and ductingtoo long.

Fit an additional fan at the end of the duct(Section 3.5).

Front and rear perforationin the work zonetotally/partially covered.

Visually check the perforation and

eliminate any type of obstruction.

2

Insufficientlaminar

down flow


Clogged laminar down flowfilter.

Check filter clogging with the cabinet innormal operation (Section 4.5 point 2).

Check the date of the last filter changeand the hours it has been operating(Section 4.5 point 4).

Notify the technical service to change thefilter.


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Check the probe position is correct (sensorparallel to the flow).

Check and eliminate any obstruction to theprobe.



3

Excessivelaminar

down flow


Air currents in frontaperture.

Check and eliminate any airflow in front ofthe cabinet that distorts the reading of thesensor.



Check and eliminate any obstruction to theprobe.

Check that the connection in the controlcard is correct (Annex 7 pos. 19).

4

Laminardown flow

not workingproperly

Alarm No. 4(see Section4.5 point J) Laminar down flow fan

stopped.

Check the fuse has not melted (AnnexA7).

Check that the connection in the controlcard is correct (Annex 7 pos. 13-14).

Notify the Technical Service to change the

fan.

Exhaust completely coveredup.

Visually check the exhaust to eliminateany type of obstruction.

Probe not working properly



Check that the exhaust box is positionedwith the perforation facing upwards.

5

Exhaust notworkingproperly


Exhaust fan stopped.

Check the fuse has not melted (AnnexA7).


Notify the Technical Service to change thefan.

6

Front doesnot move

Alarm Nos. 9and 12 (see

Section 4.5point J)

Front is blocked.

Check that no obstacle is hindering themovement of the front.

After activating Alarm No. 9 it is necessaryto press the menu key to disable the

alarm and enable the movement of thefront again.


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Fuse is melted. Replace fuse (Annex A7).

Front motor failure.Notify the Technical Service to change the

motor.Fastening cables out ofposition.

Call the Technical Service to receiveinstructions on how to adjust the cable.

Luminaire not workingproperly


Fluorescent lights notworking

Slightly turn the fluorescent light to ensuregood contact.

Change the fluorescent bulb.

7Luminairedoes notturn on

Ballast is damaged Change the ballast.

Front not in closed position Check the front is working.

UV lamp does not work

Check the lamp cap is connected properly.


Change the UV lamp.

8

UVgermicidallamp doesnot turn on

Ballast is damaged Change the ballast.

Security micro in the frontposition activated

Raise the front and check the securitymicro is not loose.

Call the Technical Service to receiveinstructions on adjusting micros.9

Intermittentalarm

activated

Alarm No. 8(see Section4.5 point J) Main control is damaged

Call a specialised technician to replace thecontrol.


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11. CERTIFICATES11.1. CESee Annex A10.1.

11.2. GUARANTEESee Annex A10.2.

Date post:	18-Oct-2015
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