BioXtra 2016 - VWR International · 2016-10-13 · UNICORN™ start 1.0 software 1 unit 29-0187-51P...

BioXtra™ 2016 Selecting the right tools can have a big impact on your research results. Explore what BioXtra offers you.

2

Even the smallest thing can have a big impact.

www.gelifesciences.com/researchtips

LiSA Life Sciences AcademyWould you or your staff benefit from a simple life sciences e-learning programme? Do you value learning in your organisation or institute? Would you like to easily standardise, automate or measure your or your team’s learning?

If you answered Yes to any of these, Life Sciences Academy is for you!

Try it out now at https://ap.lifesciences-ac.com

Selected handbooksGE Healthcare Life Sciences offers handbooks covering a wide range of techniques and applications including sample preparation, protein purification, electrophoresis, blotting, and cell culture.

Download from the link below.

www.gelifesciences.com/handbooks

VWR All-You-Need GuidesAll-You-Need collection provides customers with focused product selections, relevant to their market, including technical specifications, product descriptions.

www.vwr.com

3

Working with cells

Product descriptionOrdering quantity to

get your BioXtra priceBioXtra

ordering codeTotal list price for bundle (Ft)

BioXtra price for bundle (Ft)

Percoll 250 ml Density Gradient Media 4 packs 17-0891-02P 228 400,- 159 880,-

Percoll 1 l Density Gradient Media 4 packs 17-0891-01P 544 000,- 380 800,-

Ficoll-Paque PLUS 6 × 100 ml Density Gradient Media 4 packs 17-1440-02P 282 800,- 197 960,-

Ficoll-Paque PLUS 6 × 500 ml Density Gradient Media 4 packs 17-1440-03P 888 000,- 621 600,-

Ficoll-Paque PREMIUM 6 × 100 ml 4 packs 17-5442-02P 324 800,- 227 360,-

Ficoll-Paque PREMIUM 6 × 500 ml 4 packs 17-5442-03P 1 000 000,- 700 000,-

Cell isolationFicoll-Paque™ and Percoll™ density gradient media

Ready-to-use media for the isolation of a wide variety of cell types and organelles.

• Percoll for density gradient centrifugation of cells, viruses and subcellular particles.

• Ficoll-Paque PLUS for the isolation of mononuclear cells from human peripheral blood.

• Ficoll Paque PREMIUM for sterile, ready-to-use density gradient media for the preparation of mononuclear cells.

• Download the free Percoll calculator app at this link: gelifesciences.com/percoll-calculator

BUY NOW.

25% OFF!

http://www.gelifesciences.com/webapp/wcs/stores/servlet/catalog/en/GELifeSciences-de/service-and-support/percoll-calculator

https://xx.vwr.com/store/catalog/product.jsp?catalog_number=17-0891-01P&utm_source=flipbook&utm_medium=digital%2Bcatalog&utm_campaign=GE_bioXtra-2016






4

Working with nucleic acids

Sample preparationillustra™ RTG™ GenomiPhi™ DNA amplification kit

Kit offers a highly efficient and representative amplification of the whole genome.

• Shipping and storage at room temperature.

• 12 to 20 μg yield from nanogram amounts of DNA sample.





FTA Micro Card 100/pk, 1 sample area per card 2 packs 730-1359P 246 000,- 159 900,-

FTA Micro Card 25/pk, 1 sample area per card 2 packs 512-1162P 70 600,- 45 890,-

Indicating FTA Micro Card 100/pk, 1 sample area per card 2 packs 512-1082P 264 000,- 171 600,-

Indicating FTA Micro Card 25/pk, 1 sample area per card 2 packs 512-1163P 87 400,- 56 810,-

illustra PureTaq RTG PCR*, 0.2 ml tube, hinged cap, 96 reactions 3 packs 27-9559-01P 141 600,- 92 040,-

illustra PureTaq RTG PCR, 0.5 ml tube, 100 reactions 3 packs 27-9558-01P 138 600,- 90 090,-

illustra PureTaq RTG PCR, 0.2 ml plate, 96 reactions 3 packs 27-9557-01P 140 100,- 91 065,-

illustra GenomiPhi V2 DNA Amplification Kit, 100 reactions 2 packs 25-6600-31P 320 000,- 208 000,-

illustra GenomiPhi V2 DNA Amplification Kit, 500 reactions 2 packs 25-6600-32P 1 544 000,- 1 003 600,-

Sample collectionWhatman FTA™ sample collection cards

Whatman FTA cards provide long-term DNA stability at room temperature.

• Biosample collection.

• Transport and storage without the need for refrigeration.

• Uses and benefits of FTA demonstrated in more than 200 publications.

UP TO 30% OFF

STOCK UP

TIME TO

https://xx.vwr.com/store/catalog/product.jsp?catalog_number=512-1162P&utm_source=flipbook&utm_medium=digital%2Bcatalog&utm_campaign=GE_bioXtra-2016









5





illustra ExoProStar-1 Step (100 reactions), PCR clean-up technology 3 packs US77702P 84 300,- 54 795,-

illustra ExoProStar-1 Step (500 reactions), PCR clean-up technology 3 packs US77705P 333 000,- 216 450,-

illustra ExoProStar-1 Step (2000 reactions), PCR clean-up technology 3 packs US77720P 1 191 000,- 774 150,-

illustra RNASpin Mini (50 preps), RNA Isolation Kit 2 packs 25-0500-71P 160 800,- 112 560,-

illustra GFX PCR DNA and Gel Band Purification Kit, 100 purifications 3 packs 28-9034-70P 197 400,- 128 310,-

illustra GFX PCR DNA and Gel Band Purification Kit, 250 purifications 2 packs 28-9034-71P 302 000,- 211 400,-

illustra NAP-10 columns with Sephadex™ G-25, 50 columns 3 packs 17-0854-02P 405 000,- 263 250,-

dNTP Set, 100 mM solutions (A, C, G, T), 4 × 100 μmol 2 packs 28-4065-52P 392 000,- 274 400,-

dNTP Set, 100 mM solutions (A, C, G, T), 4 × 25 μmol 2 packs 28-4065-51P 143 600,- 100 520,-

illustra NAP-5 columns with Sephadex G-25, 50 columns 3 packs 17-0853-02P 390 000,- 253 500,-

illustra NAP-25 columns with Sephadex G-25, 50 columns 3 packs 17-0852-02P 468 000,- 304 200,-

PCR* and clean-upillustra ExoProStar™ for PCR clean-up

Enzymatic PCR clean-up technology with illustra Exonuclease I and Alkaline Phosphatase to remove unincorporated primers and dNTPs.

• Fast (30 min) and easy to automate.

• No loss of PCR product.

Purificationillustra GFX™ PCR DNA and Gel Band Purification Kit

Isolation and concentration of DNA fragments from PCR mixtures, agarose gel bands, and enzyme digests in under 10 min.

• Flexible elution volume for different DNA concentrations.

• DNA for use in sequencing, PCR, labeling, digestion, and cloning.

* See legal information on back page.

30% OFF!

UP TO

https://xx.vwr.com/store/catalog/product.jsp?catalog_number=US77705P&utm_source=flipbook&utm_medium=digital%2Bcatalog&utm_campaign=GE_bioXtra-2016











6

Protein purification



ordering codeTotal list price (Ft)

BioXtra price (Ft)

ÄKTA start – main instrument 1 unit 29-0220-94P 2 187 000,- 1 666 000,-

Frac30 Fraction Collector 1 unit 29-0230-51P 429 000,- 365 650,-

UNICORN™ start 1.0 software 1 unit 29-0187-51P 278 000,- 236 360,-

Purification systemÄKTA™ start chromatography system

ÄKTA start is an affordable, easy-to-use protein purification system that allows you to automate manual protein purification procedures in academic and educational labs.

• Intuitive touch-screen display and real-time monitoring.

• Compact solution for quick and reliable one-step purification.

• Quick start methods and templates for common purification techniques.




7





HiPrep™ 16/60 Sephacryl S-100 HR 1 pack 17-1165-01P 207 000,- 155 250,-

HiPrep 16/60 Sephacryl S-200 HR 1 pack 17-1166-01P 207 000,- 155 250,-





Size exclusion chromatographySephacryl™ high resolution media

• Convenient, easy-to-use, prepacked gel filtration columns in two different column sizes.

• Choice of five selectivities covering a wide molecular weight range.

• Reliable and reproducible preparative purification.

• Easy connection to ÄKTA chromatography systems.

SephacrylLow/medium-pressure system Macromolecule separation Product line covering wide fractionation range

106 105 104M (approx.)

12

3

45

1

34 5

6 7

7

6

2

3 45

6

12

S-300

S-100

S-200

r

1. Thyroglobulin (Mr 669 000)2. Ferritin (Mr 440 000)3. Aldolase (Mr 158 000)4. Albumin (Mr 67 000)5. Ovalbumin (Mr 43 000)6. Chymotrypsinogen A (Mr 25 000)7. Ribonuclease A (Mr 13 700)

BUY NOW!

20% OFF!







8



ordering codeTotal list price (Ft)

BioXtra price (Ft)

HisTrap HP, 5 × 1 ml columns 1 pack 17-5247-01P 63 000,- 47 250,-

HisTrap HP, 5 × 5 ml columns 1 pack 17-5248-02P 216 000,- 162 000,-

Ni Sepharose HP, 25 ml 1 pack 17-5268-01P 131 000,- 98 250,-

HisTrap FF, 5 × 1 ml columns 1 pack 17-5319-01P 50 800,- 38 100,-

HisTrap FF, 5 × 5 ml columns 1 pack 17-5255-01P 177 000,- 132 750,-

HisTrap FF crude, 5 × 1 ml columns 1 pack 11-0004-58P 55 300,- 41 475,-

HisTrap FF crude, 5 × 5 ml columns 1 pack 17-5286-01P 189 000,- 141 750,-

Ni Sepharose 6 FF, 25 ml 1 pack 17-5318-01P 107 000,- 80 250,-

HisTrap excel, 5 × 1 ml columns 1 pack 17-3712-05P 68 300,- 51 225,-

HisTrap excel, 5 × 5 ml columns 1 pack 17-3712-06P 230 000,- 172 500,-

Ni Sepharose excel, 25 ml 1 pack 17-3712-01P 131 000,- 98 250,-

HiTrap TALON crude, 5 × 1 ml columns 1 pack 28-9537-66P 55 300,- 41 475,-

HiTrap TALON crude, 5 × 5 ml columns 1 pack 28-9537-67P 190 000,- 142 500,-

TALON Superflow, 10 ml 1 pack 28-9574-99P 48 300,- 36 225,-

Histidine-tagged protein purificationNi Sepharose

• Ni Sepharose FF and HP media and HisTrap™ HP and FF provide high binding capacity for your histidine-tagged proteins.

• HisTrap excel and Ni Sepharose excel will minimize Ni-leakage, even when stripping agents are present in the sample.

• The "crude" pre-packed columns offer one-step purification directly from homogenized, unclarified cell lysate.

TALON® Superflow™

• Cobalt-based TALON products are the first choice when the purity of your histidine-tagged protein is more important than yield.

• TALON Superflow binds histidine-tagged proteins with an enhanced specificity: it binds fewer unwanted proteins.















9





GSTrap™ HP, 5 × 1 ml columns 1 pack 17-5281-01P 104 000,- 78 000,-

GSTrap HP, 5 × 5 ml columns 1 pack 17-5282-02P 399 000,- 299 250,-

GSTrap 4B, 5 × 1 ml columns 1 pack 28-4017-45P 87 200,- 65 400,-

GSTrap 4B, 5 × 5 ml columns 1 pack 28-4017-48P 324 000,- 243 000,-

Glutathione Sepharose 4B, 10 ml 1 pack 17-0756-01P1 84 400,- 63 300,-

Glutathione Sepharose 4B, 10 ml 2 packs 17-0756-01P2 168 800,- 113 096,-

GSTrap FF, 5 × 1 ml columns 1 pack 17-5130-01P 93 100,- 69 825,-

GSTrap FF, 5 × 5 ml columns 1 pack 17-5131-02P 353 000,- 264 750,-

Glutathione Sepharose 4 FF, 25 ml 1 pack 17-5132-01P 193 000,- 144 750,-

Factor Xa (protease used to digest fusion proteins, 400 units) 1 pack 27-0849-01P 164 000,- 123 000,-

PreScission™ Protease (protease used to digest fusion proteins, 500 units) 1 pack 27-0843-01P 93 000,- 69 750,-

Thrombin (protease used to digest fusion proteins, 500 units) 2 packs 27-0846-01P 85 200,- 63 900,-

PD 10 Desalting Columns, 30 columns 3 packs 17-0851-01P 303 000,- 227 250,-

PD MiniTrap G-10 1 pack 28-9180-10P 101 000,- 75 750,-

PD MiniTrap G-25 1 pack 28-9180-07P 101 000,- 75 750,-

PD MidiTrap G-10 1 pack 28-9180-11P 114 000,- 85 500,-

PD MidiTrap G-25 1 pack 28-9180-08P 101 000,- 75 750,-

GST-tagged protein purificationGlutathione Sepharose

• Fast and simple one-step purification of GST-tagged proteins.

• Easy scale-up by connecting up to three HiTrap columns in series.

Manual desaltingSingle-use columns for buffer exchange and cleanup of biological samples

• Convenient and rapid cleanup with high reproducibility.

• A wide range of applications including desalting, buffer exchange, and the removal of low-molecular weight compounds.

• High desalting capacity.

• The use of centrifugation protocol with all gravity columns.

SAVE UP

TO 25%




https://xx.vwr.com/store/catalog/product.jsp?catalog_number=17-0756-01P1&utm_source=flipbook&utm_medium=digital%2Bcatalog&utm_campaign=GE_bioXtra-2016














10





HiTrap Streptavidin HP, 5 × 1 ml columns 1 pack 17-5112-01P 177 000,- 132 750,-

Streptavidin Sepharose HP, 5 ml 1 pack 17-5113-01P 142 000,- 106 500,-

StrepTrap HP, 1 × 1 ml 1 pack 29-0486-53P 49 700,- 37 275,-

StrepTrap HP, 5 × 1 ml columns 1 pack 28-9075-46P 198 000,- 148 500,-

StrepTrap HP, 1 × 5 ml 1 pack 28-9075-47P 125 000,- 93 750,-

StrepTrap HP, 5 × 5 ml columns 1 pack 28-9075-48P 524 000,- 393 000,-

StrepTactin Sepharose High Performance 10 ml 1 pack 28-9355-99P 201 000,- 150 750,-

StrepTactin Sepharose High Performance 50 ml 1 pack 28-9356-00P 894 000,- 670 500,-

Strep-tag protein purificationStraptavidin and Streptactin Sepharose

Biotin and biotinylated substances bind to streptavidin, a molecule isolated from Streptomyces avidinii. The binding of streptavidin to biotin is one of the strongest known non-covalent biological interactions.

Strep-tag II binds very specifically to the immobilized Strep-Tactin ligand, giving highly pure target protein after purification. In most cases, sufficient purity is obtained in single-step affinity purification.

• Streptavidin Sepharose allows binding of biotinylated substances.

• Streptactin Sepharose will deliver highly pure Strep(II)-tagged recombinant proteins eluted in concentrated form and small volumes.

• Physiological conditions and mild elution preserve target protein activity.

• Fast and easy regeneration with 0.5 M NaOH.

20%

SAVINGS









11

Protein purification information base Visit the protein purification community

Discussion forum – share insights and ask questions related to protein purification

Knowledge library – including downloads, videos and other resources

Products for proteins – browse tools for protein purification including ÄKTA systems and prepacked columns

Protein blog and news – keep up to date with latest news and events

ÄKTA club – access to information about your ÄKTA system has never been so easy.

proteins.gelifesciences.com

GE Purify App

Need further guidance? Protein purification made easy: The Purify app simplifies the purification process by helping you to quickly select the right product(s) for your needs.

Download the free Purify App at gelifesciences.com/purify

ÄKTA club

TODAY

JOIN

http://www.gelifesciences.com/purify

12

Blotting





Amersham ECL Rainbow Marker - Full Range (Mr 12 000 to 225 000), 250 μl 2 packs RPN800EP 173 000,- 86 500,-

Amersham ECL Rainbow Marker - High Range (Mr 12 000 to 225 000), 250 μl 2 packs RPN756EP 142 800,- 71 400,-

Amersham ECL Rainbow Marker - Low Range (Mr 3 500 to 38 000), 250 μl 2 packs RPN755EP 140 000,- 70 000,-

3MM Chr Blotting Paper, 46 × 57 cm, 100 sheets/pk 3 packs 514-8013P 306 000,- 122 400,-



Hybond Blotting Paper, 20 × 20 cm, 100 sheets/pk 2 packs RPN6101MP 41 800,- 31 350,-

Blotting paperGelMembraneBlotting paper

Gel electrophoresisAmersham™ ECL™ Rainbow™ Markers

• Ready-to-load markers with sharp, intense bands on gels and blots enable monitoring of electrophoresis progress and convenient confirmation of transfer to blotting membranes.

• Comprises of 10 individually coloured proteins in the molecular weight range of 12 000 to 225 000.

• Each 250 μl pack size is sufficient for use with 50 minigels (10 × 8 cm) or 25 large gels (20 × 20 cm).

Blotting papersWhatman™ 3MM Chr blotting papers

• Pure cellulose produced entirely from the highest quality cotton linters with no additives of any kind.

• Ensures that no contamination will occur during the transfer steps.

• Whatman 3MM Chr considered the industry standard for blotting procedures.

UP TO 50% OFF!STOCK UP NOW!

https://xx.vwr.com/store/catalog/product.jsp?catalog_number=RPN800EP&utm_source=flipbook&utm_medium=digital%2Bcatalog&utm_campaign=GE_bioXtra-2016




https://xx.vwr.com/store/catalog/product.jsp?catalog_number=RPN6101MP&utm_source=flipbook&utm_medium=digital%2Bcatalog&utm_campaign=GE_bioXtra-2016



13





Amersham Protran 0.45 μm NC, 300 mm × 4 m, 1 roll/pk 2 packs 10-6000-02P 182 800,- 118 820,-


Amersham Protran Premium 0.45 μm NC, 300 mm × 4 m, 1 roll/pk 2 packs 10-6000-03P 187 600,- 121 940,-


Amersham Protran Supported 0.45 μm NC, 300 mm × 4 m, 1 roll/pk 2 packs 10-6000-16P 218 000,- 141 700,-

Amersham Protran Supported 0.45 μm NC, 200 mm × 4 m, 1 roll/pk 2 packs 10-6000-18P 161 800,- 105 170,-

Amersham Hybond P 0.45 μm PVDF, 300 mm × 4 m, 1 roll/pk 2 packs 10-6000-23P 204 000,- 132 600,-

Amersham Hybond P 0.2 μm PVDF, 260 mm × 4 m, 1 roll/pk 2 packs 10-6000-21P 224 000,- 145 600,-

Amersham Hybond LFP 0.2 μm PVDF, 254 mm × 4 m, 1 roll/pk 2 packs 10-6000-22P 332 000,- 215 800,-

Amersham Hybond LFP 0.2 μm PVDF, 200 mm × 200 mm, 10 sheets/pk 2 packs 10-6000-60P 184 200,- 119 730,-

BlottingAmersham Protran™ nitrocellulose (NC) blotting membranes

• Amersham Protran membranes offer the advantage of low background in Western blotting, regardless of detection method used.

• Well-suited for applications where X-ray film is used for detection.

• Exceptional handling characteristics of Amersham Protran Supported NC membranes allows for stripping and reprobing of the membrane.

Amersham Hybond™ PVDF blotting membranes

High protein binding capacity results in high sensitivity.

• Suitable for Western blotting applications with stripping and reprobing.

• Amersham Hybond P recommended for chemiluminescence detection.

• Amersham Hybond LFP specifically optimized for fluorescence detection methods.

SAVE 20%











14





Amersham ECL Rabbit IgG, HRP-linked whole Ab (from donkey), 1 ml 2 packs NA934-1MLP 266 000,- 172 900,-

Amersham ECL Mouse IgG, HRP-linked whole Ab (from sheep), 1 ml 2 packs NA931-1MLP 268 000,- 174 200,-

Streptavidin-Biotinylated Horseradish Peroxidase Complex 2 ml 1 pack RPN1051-2MLP 145 000,- 101 500,-

Streptavidin-Alkaline Phosphatase Conjugate 2 ml 1 pack RPN1234P 178 000,- 124 600,-

Anti-GST HRP Conjugate 75 μl 1 pack RPN1236P 92 300,- 64 610,-

Amersham ECL start Western Blotting Detection Reagent, 200 ml 2 packs RPN3243P 76 200,- 45 720,-

Amersham ECL start Western Blotting Detection Reagent, 400 ml 2 packs RPN3244P 122 000,- 73 200,-

Amersham ECL Western Blotting Detection Reagent for 2000 cm2, 250 ml 2 packs RPN2209P 162 400,- 97 440,-

Amersham ECL Western Blotting Detection Reagent for 4000 cm2, 500 ml 2 packs RPN2106P 210 000,- 126 000,-

Amersham ECL Prime Western Blotting Detection Reagent for 1000 cm2, 100 ml 2 packs RPN2232P 168 800,- 101 280,-

Amersham ECL Select Western Blotting Detection Reagent for 1000 cm2, 100 ml 2 packs RPN2235P 210 000,- 126 000,-

Amersham Hyperfilm ECL 18 × 24 cm, 50 sheets/pk 3 packs 28-9068-36P 216 300,- 118 965,-

Amersham Hyperfilm ECL 18 × 24 cm, 100 sheets/pk 3 packs 28-9068-37P1 357 000,- 196 350,-

Amersham Hyperfilm ECL 18 × 24 cm, 100 sheets/pk 5 packs 28-9068-37P2 595 000,- 267 750,-

Amersham Hyperfilm ECL 5 × 7 inches, 50 sheets/pk 3 packs 28-9068-35P 188 700,- 103 785,-

Amersham Hyperfilm MP 18 × 24 cm, 100 sheets/pk, high sensitivity 3 packs 28-9068-44P 357 000,- 196 350,-

DetectionAmersham ECL start Western blotting detection reagent

GE recently launched Amersham ECL start – a new reliable chemiluminescence WB substrate.

• Flexibility: long signal duration provides assay design flexibility and robust detection of large experimental sets.

• Low background: regardless of the detection method.

• Stability: working solution stable for 5 days.

• Shelf life: 18 months shelf life.

ImagingAmersham Hyperfilm™ ECL detection film

Sensitive film for the detection of chemiluminescent signals in Western blotting assays.

• Clear background for excellent contrast and band visibility.

• Publication-quality images.

• Learn more here: www.gelifesciences.com/wbfaq

50% OFF!

UP TO

http://www.gelifesciences.com/wbfaq

https://xx.vwr.com/store/catalog/product.jsp?catalog_number=NA934-1MLP&utm_source=flipbook&utm_medium=digital%2Bcatalog&utm_campaign=GE_bioXtra-2016

https://xx.vwr.com/store/catalog/product.jsp?catalog_number=RPN1051-2MLP&utm_source=flipbook&utm_medium=digital%2Bcatalog&utm_campaign=GE_bioXtra-2016

https://xx.vwr.com/store/catalog/product.jsp?catalog_number=RPN1234P&utm_source=flipbook&utm_medium=digital%2Bcatalog&utm_campaign=GE_bioXtra-2016









https://xx.vwr.com/store/catalog/product.jsp?catalog_number=NA931-1MLP&utm_source=flipbook&utm_medium=digital%2Bcatalog&utm_campaign=GE_bioXtra-2016





15

GE Healthcare Life Sciences offers a broad selection of nitrocellulose (NC) and polyvinylidene difluoride (PVDF) Western blotting membranes, with pore sizes ranging from 0.1 to 0.45 μm to suit your application requirements. By bringing together the trusted Hybond and Whatman brands, Amersham Western blotting membranes offer sustained high quality and performance.

A comparison table outlining the differences between ECL reagents.

Selection guides

Nitrocellulose membranes New names

Whatman Protran BA85, BA83 and BA79 → Amersham Protran NC

Amersham Hybond ECL → Amersham Protran NC Premium

Whatman Optitran BAS85 and BAS83 → Amersham Protran NC Supported

PVDF membranes New names

Amersham Hybond P 0.2 PVDF

Amersham Hybond P and Whatman Westran CS → Amersham Hybond P 0.45 PVDF

Amersham Hybond LFP → Amersham Hybond LFP 0.2 PVDF

Whatman Westran S → Amersham Hybond SEQ 0.2 PVDF

Two great brands join forces

Amersham ECL Western blotting detection reagents selection guide

NEW

AmershamWestern blotting

membranes

HybondProtranOptitran

Westran S

Amersham ECL

Reliable detection of high to medium abundant proteins

Chemiluminescence

Amersham Protran

Amersham Hyperfilm (X-ray film) or ImageQuant LAS 500

< 2 h

• Routine protein detection

• Protein confirmation• Overexpressed proteins• Tagged proteins

Amersham ECL Prime

Quantitative analysis of high to low abundant proteins

Chemiluminescence

Amersham Hybond PAmersham Protran

Amersham Imager 600 series X-ray film is also an option

< 24 h

• Protein quantitation• Protein confirmation• Protein regulation and

expression analysis

Amersham ECL Select

Quantitative analysis of medium to very low abundant proteins

Chemiluminescence

Amersham Hybond PAmersham Protran

Amersham Imager 600 series X-ray film is also an option

< 2 h

• Protein quantitation• Protein confirmation• Protein regulation and

expression analysis

Amersham ECL Plex™

Multiplex protein analysis and quantitation of high to very low protein levels

Fluorescence

Amersham Hybond LFPAmersham Protran Premium

Typhoon™ FLA seriesAmersham Imager 600RGB

> 3 months

• Multiplex analysis• Normalization to

housekeeping protein• Analysis of post

translational modifications and isoforms

Amersham ECL start

Detection of high to medium abundant proteins

Chemiluminescence

Amersham Protran

Amersham Hyperfilm (X-ray film) or ImageQuant™ LAS 500

< 3 h

• Routine protein detection• Highly expressed

proteins e.g. housekeeping proteins

• Tagged proteins

Product

Detection type

Optimal membrane

Recommended detection

Signal duration

Typical applications

Typical results

WB ERK ½; LOD: 20 ng; DR: 2.4

20 ng5 µg


5 ng1.2 µg

WB ERK ½ (Cy5 on FLA 9500); LOD 10 ng; DR: 3

5 ng10 µg


40 ng5 µg

WB ERK ½: LOD 320 ng; DR

320 ng10 µg

GE and GE monogram are trademarks of General Electric Company.

ÄKTA, Amersham, Benchkote, BioXtra, ECL, ECL Plex, ExoProStar, FTA, Ficoll-Paque, GenomiPhi, GFX, GSTrap, HiPrep, HisTrap, HiTrap, Hybond, Hyperfilm, illustra, ImageQuant, Percoll, PreScission, Protran, Rainbow, RTG, Sephadex, Sepharose, SPARTAN, Superdex, Superose, Typhoon, UNICORN, and Whatman are trademarks of General Electric Company or one of its subsidiaries.

VWR is a trademark of VWR International, LLC. GeneQuant, NovaSpec, and Ultrospec are trademarks of Biochrom Ltd. TALON is a trademark of Clontech Laboratories Inc. Superflow is a trademark of Sterogene Bioseparations Inc. All other third-party trademarks are the property of their respective owners.

*The Polymerase Chain Reaction (PCR) is covered by patents owned by Roche Molecular Systems and F Hoffmann-La Roche Ltd. A license to use the PCR process for certain research and development activities accompanies the purchase of certain reagents from licensed suppliers such as GE Healthcare and affiliates when used in conjunction with an authorized thermal cycler.

Phi 29 DNA polymerase and its use for DNA synthesis is covered by US patent numbers 5,854,033, and 5,576,204.

Ready to Go RT-PCR Beads: Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, and 6,127,155. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser’s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

© 2016 General Electric Company—All rights reserved. First published June 2016.

All offers in this issue are valid until 31st December 2016. All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them. A copy of these terms and conditions is available on request.

Contact your local GE Healthcare representative for the most current information.

Every effort will be made to give reasonable notice of price changes but we reserve the right to change prices without further notice. All prices exclude value added or sales tax and may be subject to change without notice. These offers cannot be used in conjunction with any previously arranged purchase agreement.

Go to www.vwr.com for the latest news, special offers and details of your local VWR distributor

06/2016 HUN

VWR contact informationAustriaVWR International GmbHGraumanngasse 71150 ViennaTel.: +43 1 97 002 0Fax: +43 1 97 002 600Email: [email protected]

BelgiumVWR International bvbaResearchpark Haasrode 2020Geldenaaksebaan 4643001 LeuvenTel.: 016 385 011Fax: 016 385 385Email: [email protected]

Czech RepublicVWR International s. r. o. Veetee Business Park Pražská 442CZ - 281 67 Stříbrná Skalice Tel.: +420 321 570 321 Fax: +420 321 570 320 Email: [email protected]

DenmarkVWR - Bie & BerntsenTransformervej 82860 SøborgTel.: 43 86 87 88Fax: 43 86 87 90Email: [email protected]

FinlandVWR International OyValimotie 900380 HelsinkiTel.: 09 80 45 51Fax: 09 80 45 52 00Email: [email protected]

FranceVWR International S.A.S.Le Périgares – Bâtiment B201, rue Carnot94126 Fontenay-sous-Bois cedexTel: 0 825 02 30 30 (0,18 € TTC/min)Fax: 0 825 02 30 35 (0,18 € TTC/min)Email: [email protected]

GermanyVWR International GmbHHilpertstraße 20aD - 64295 DarmstadtFreecall: 0800 702 00 07Fax: 0180 570 22 22*Email: [email protected]*0,14 €/Min. aus d. dt. Festnetz

HungaryVWR International Kft.Simon László u. 4.4034 DebrecenTel.: (52) 521-130Fax: (52) 470-069Email: [email protected]

Ireland / Northern IrelandVWR International Ltd / VWR International (Northern Ireland) LtdOrion Business CampusNorthwest Business ParkBallycoolin, Dublin 15Tel.: 01 88 22 222Fax: 01 88 22 333Email: [email protected]

ItalyVWR International S.r.l.Via San Giusto 8520153 Milano (MI)Tel.: 02-3320311Fax: 800 152999/02-40090010Email: [email protected]

The NetherlandsVWR International B.V.Postbus 81981005 AD AmsterdamTel.: 020 4808 400Fax: 020 4808 480Email: [email protected]

NorwayVWR International ASHaavard Martinsens vei 300978 OsloTel.: 02290Fax: 815 00 940 Email: [email protected]

PolandVWR International Sp. z o.o.Limbowa 5, 80-175 GdanskTel.: 058 32 38 200 Fax. 058 32 38 205Email: [email protected]

PortugalVWR International - Material de Laboratório, LdaEdifício NeoparkAv. Tomás Ribeiro, 43- 3 D2790-221 CarnaxideTel.: 21 3600 770Fax: 21 3600 798/9Email: [email protected]

SpainVWR International Eurolab S.L.C/ Tecnología 5-17A-7 Llinars Park08450 - Llinars del VallèsBarcelonaTel.: 902 222 897Fax: 902 430 657Email: [email protected]

SwedenVWR International ABFagerstagatan 18a163 94 StockholmTel.: 08 621 34 00Fax: 08 621 34 66Email: [email protected]

SwitzerlandVWR International GmbHLerzenstrasse 16/188953 DietikonTel.: 044 745 13 13Fax: 044 745 13 10Email: [email protected]

UKVWR International LtdCustomer Service CentreHunter Boulevard - Magna ParkLutterworthLeicestershireLE17 4XNTel.: 0800 22 33 44Fax: 01455 55 85 86Email: [email protected]

Date post:	05-Apr-2020
Category:	Documents
Upload:	others
View:	10 times
Download:	0 times

BioXtra 2016 - VWR International · 2016-10-13 · UNICORN™ start 1.0 software 1 unit 29-0187-51P...

Documents