BipureDaily skin champion

INTERNAL INSULTS

Daily Invisible Invaders

EXTERNAL INSULTS

Insults accumulation

+ GLYCATION

+ R.O.S.

+AGEs

+FREE RADICALS

+OXIDATIVE STRESS

Combined skin outcomes

Ageing process

acceleration

Hyper sensitivity

Dermis structure

degradation

Inflammation

Wrinkles and lines

Skin darkening

Saggy skin

Older appearance

Among all the environmental

factors, UV exposure

accounts for up to 80% of

both free radicals and

Reactive Oxygen Species

(ROS) release *.

*Poljšak B. and Dahmane R., “Free Radicals and Extrinsic Skin Aging”, Derm. Res. and Pract. 2012

UV EXPOSURE

By-products of the normal

metabolism of oxygen

Very small molecules

Highly reactive

Increased by environmental

stress

OXIDATIVE STRESS

Damage to cell structures

Inflammation

=

AGEING

ROS

Loss of elasticity

and changes in the dermis

=

AGEING

GLYCATIONGlycation processBefore

After

no enzyme required

ROS

AGEs

Advanced Glycation End-products

cross-linked proteins

infiammation

BipureInci Name: Azelaoyl Bis-Dipeptide-10, Citrate Buffer, Glycerin

Azelaic Acid

L- AlanineL- Histidine L- Histidine

L- Alanine

Mechanism of action

GLYCATION PREVENTION (AGEs)

Bipure molecular structure prevents thebinding of sugar to proteins,

inhibiting AGEs formation and consequent skin lipid peroxidation.

OXIDATIVE STRESS INHIBITION (ROS)

Bipure molecular structure captures oxidants protecting skin from

damaging.

TEST in vitro

TESTS

TEST in vivo

UVA-INDUCED OXIDATIVE STRESS

INHIBITION

Bipure is effective in inhibiting up to 41% UVA-induced ROS.

At a concentration of 6,25% , Bipure shows a 28% scavenging

activity even after a 12 minutes UVA exposure.

In vitro

-45

-40

-35

-30

-25

-20

-15

-10

-5

0

0

-26

-28

-12

-34

-37

-18

-37

-41

UV

A i

nd

uce

d R

OS

%

Bipure 1,56% Bipure 3,12% Bipure 6,25%

12’ UVA

8’ UVA

4’ UVA

-41%

-45

-40

-35

-30

-25

-20

-15

-10

-5

0

-16 -16-18

-29 -29

-40

-37

-40-42

En

do

ge

no

us

RO

S %

Bipure 1,56% Bipure 3,12% Bipure 6,25%

T12'

T16'

T20'

ENDOGENOUS OXIDATIVE STRESS

INHIBITION

Bipure is effective in inhibiting up to 42% endogenous ROS.

-42%

In vitro

0

0,01

0,02

0,03

BIPURE 1% BIPURE 0,5% Negative control

µg

AG

Es/

µg

pro

tein

AGEs

Bipure is able to halve AGEs production on human

fibroblasts.

-50%-50%

ANTI GLYCATION (AGEs) In vitro

Efficacy on overall

skin health parameters

Protocol

12 women, Caucasian, mean age 51,2 years

T0, T3, T6

Twice a day for 6 weeks

Face

Bipure emulsion 5% Vs placebo

Elasticity, hydration, skin density, brightness

Skin roughness, smoothness profilometry

ISPE Institute of Skin and Product Evaluation, Milan, Italy

In Vivo

Efficacy results

3D Photo image by Primos

Before treatment After 3 weeks

After a 3-week-treatment , 5% Bipure emulsion shows an improvement of profilometric

parameters: wrinkles appear less evident and deep; skin elasticity, density and brightness

are enhanced.

In Vivo

TECHNICAL DESCRIPTION

INCI Name:

Aspect:

Colour:

Odour:

Dry residue:

Recommended use:

Azelaoyl Bis-Dipeptide-10,

Citrate Buffer, Glycerin

Liquid

White to pale yellow

Characteristic

23 -28%

2-5%

17

Features

Anti oxidant

Radical

scavenger

Glycation

rescuer

Pollution

defender

Youth guardian Youth guardian

Skin firewall

Bipure

18

Applications

Urban daily

serum

Bipure

Face PrimerBB, CC, DD

cream

Tox-free

cleanser

Night reload

mask

Pollution

shieldDetox cream

On demand info

Clinical tests

Formulations

Technical Sheets

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