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Ribosome Lectures: 1. Types and roles of RNA 2. Structure and function of RNA 3. Regulation of mRNA translation 4. Non-coding RNA 5. Riboswitches and Ribozymes 6. RNA in biotechnology Labs: 1. Targeting an RNA of interest 2. Chemical probing extracted RNA 3. Chemical probing in-cell RNA 4. High-throughput sequencing 5. Chemical reactivity analysis 6. Modeling RNA structures BIT 495/595 RNA World Structure Function Secondary structure RNA Higher-order structure Ribozyme Riboswitch RNA molecules have a diverse range of functions. Many RNA functions depend on secondary and higher-order structure. • Protein synthesis mRNA, rRNA, tRNA • Regulatory RNA e.g. lncRNA, miRNA • DNA replication & post-transcriptional modification e.g. snRNA, smYRNA • Parasitic RNA e.g. Viral genome, viroid, retrotransposons. tmRNA SRP RNA IRES Transfer RNA snRNA
