BMD Mikro.pptx

7/25/2019 BMD Mikro.pptx

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MIKROBIOLOGI

Mekanisme Dasar

Penyakit

Bagian Mikrobiologi

Fakultas Kedokteran Universitas Hasanuddin

2014


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KEL ASISTEN NIM ( C 111 ....)

1 MUHAMMAD FUDAIL 09 136, 10 110, 13 00113 0!6

! "RI #AH$U%I R& 10 13', 10 !63, 13 0!(13 0)3

3 *OR+I% KUR%IA#A% & 10 !!(, 10 !'0, 13 0)-13 0((

- A& MUH& FADLILLAH F& 10 !99, 10 30', 13 0('

13 10!) MAR$AM BURHA%UDDI% 10 31-, 10 33!, 13 103

13 30)

6 MUIAH MUFIHAURRAHMA% 10 3!-, 10 3!6, 13 30(13 33-

( MUH& RAHMA RIDHA 10 3!', 10 33!, 13 33)13 36(

' #A% A"H.KAL %A/UA% BI% #A%A/MI

10 33-, 13 36'13 )!(

9 HAFI/ HA/#A% MOHAMMAD %ORI/A% 10 339, 13 )!'13 ))-

10 "II AI"HAH KHAIRUDI% 10 3-6, 13 ))(13 (0-


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TATA-TERTIB LABORATORIUMMIKROBIOLOGI

Laki laki : Memakai eana 2anan4 kain, n5 255 sirt,ram78t tiak 75e kena kera 7a8

Perem!a" :Memakai r5k 2anan4 kain, n5 255 sirt,

ram78t iikat ke atas& Ba4i y4 7eri7a7, i7a7 imas8kkani aam as a7 Ala# $a"% &ar!' ia*a 'aa# rak#ik!m :

*as a7

ID :ar

Pen8nt8n 2raktik8m ; F5t5 -


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BAK.RI

Bakteri aaa ma8k i82,7erse t8n44a kei en4an

imeter kirakira 1 m, yan4mas8k aam 455n4an 2r5tistarena, Pr5kari5tik, Inti anya

7r2 m7aran kr5m5s5m tan2amem7ran inti


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"e Bakteri


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"RUKUR BAK.RI

Dinin4 "e Mem7ran "e "it52asma %8e5si Ka2s8

Fa4e Pii>Fim7riae "25ra


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Fa4e


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M5r?554i Bakteri

58s

7aii

i255i

stre2t55i

.


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sarina

Lan8tan

sta2y55i tettraen

557aii

.


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Lan8tan

Fiament58s?5rm

i7ri5

F8si?5rm 7aii

s2ira

.


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+ia%",'i"% i" Mir,i,l,%$

"2eimen @ De2en 5n sym2t5ms an timin4 Urine, 755, 28s, s2ina C8i, s28t8m, 5r st55

P.#AR%AA% :8t8res EB55 A4ar,%8trient a4ar, BHIA,

Ma:5nkey, "" A4ar, :B" Bi5emia ests E"I, "IM, +P, Mety Re,

:itrat, Urea, KH test %8ei Ai Met5s EP:R, y7riiati5n,

75ts Anti75y Meiate E.LI"A, sie>ate+OG." PRO"KAU.R EMR>+P

Mety Re

E; @Mera E @

+54es Pr5ska8er

E; @ Mera E @

Tujuan : Untuk mengetahui apakah bakteri memfermentasikanglukosa atau tidak.


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:IRA. UR.A H$DROL$"I"

:itrate

E; @ Bir8 E @ Hia8

Urea Hyr5ysis

E; @Pink E @ K8nin4

"UGAR F.RM.%AIO%


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"UGAR F.RM.%AIO%."

Lat5seFermentati5n

"8r5se

Fermentati5n G85se

Fermentati5n Mannit5

E; @ K8nin4 E @ Mera

Tube 1: Negative acid /Negative gas

Tube 2A: Must incubate longer (ambiguous result)

Tube 2B: Positive acid /Negative gas

Tube 3A: Positive acid/ Positive gas


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Special selective and diferential agar medium:

1.

M C,"ke$ a%arla#e0. SS a%ar la#e

5. TCBS


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Mc Conkey Agar

MaC,"ke$ a%ar is a 8t8re mei8mesi4ne t5 4r5= Gramne4atie7ateria an stain tem ?5r at5se?ermentati5n&

In4reients@ Bile Sal# Et5 ini7it m5st Gram25sitie

7ateria, e


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Ma5nkey a4ar =itat5seEe?t an n5n

at5seEri4t ?ermenters


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PRI%:IPL.

La#,'e By 8tiiin4 te at5se aaia7e in te

mei8m, La; 7ateria s8 asEscherichia coli, Enterobacter anKlebsiella =i 2r58e ai, =i5=ers te 2H 5? te a4ar 7e5= 6&' anres8ts in te a22earane 5? re>2ink

55nies& e 7ie sats 2rei2itate in teimmeiate nei475r55 5? te 55ny,a8sin4 te mei8m s8rr58nin4 te55ny t5 7e5me ay&
http://en.wikipedia.org/wiki/Escherichia_colihttp://en.wikipedia.org/wiki/Enterobacterhttp://en.wikipedia.org/wiki/Klebsiellahttp://en.wikipedia.org/wiki/Acidhttp://en.wikipedia.org/wiki/Bacterial_colonyhttp://en.wikipedia.org/wiki/Bacterial_colonyhttp://en.wikipedia.org/wiki/Acidhttp://en.wikipedia.org/wiki/Klebsiellahttp://en.wikipedia.org/wiki/Enterobacterhttp://en.wikipedia.org/wiki/Escherichia_coli


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La#,'e- %5nLat5se ?ermentin4 7ateria s8 as

Salmonella, Proteus species,

Pseudomonas aeruginosa an Shigella ann5t8tiie at5se, an =i 8se 2e2t5ne instea&

is ?5rms amm5nia, =i raises te 2H 5?te a4ar, an eas t5 te ?5rmati5n 5?

=ite>55ress 55nies ?5rme in te 2ate&B8t tey an as5 55k 45en t5 7r5=n =itark enters& ey are ir8ar 55nies anarran4e ran5my&
http://en.wikipedia.org/wiki/Salmonellahttp://en.wikipedia.org/wiki/Proteus_(bacterium)http://en.wikipedia.org/wiki/Pseudomonas_aeruginosahttp://en.wikipedia.org/wiki/Shigellahttp://en.wikipedia.org/wiki/Peptonehttp://en.wikipedia.org/wiki/Ammoniahttp://en.wikipedia.org/wiki/Agarhttp://en.wikipedia.org/wiki/Agarhttp://en.wikipedia.org/wiki/Ammoniahttp://en.wikipedia.org/wiki/Peptonehttp://en.wikipedia.org/wiki/Shigellahttp://en.wikipedia.org/wiki/Pseudomonas_aeruginosahttp://en.wikipedia.org/wiki/Proteus_(bacterium)http://en.wikipedia.org/wiki/Salmonella


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SS agar plate:

In4reients@ %8trient a4ar 100m Lat5se 1&004 Bie sats 0&')4

ini7it5r

"5i8m itrate 0&')4ini7it5r

"5i8m ti5s8?ate 0&')4

10 ?erri itrate 1m 1 %e8tra re 0&!)m

iniat5r

1 7riiant 4reen 0&033mini7it5r


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(


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(T&i,'!l/a#e-Ci#ra#e-Bile-S!r,'e)

Use ?5r8t8re Vibriocholerae

$e5= 55nies?5r i7ri5s 8et5 5


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TCBS A%ar: $east .


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e 7ie sats in te meia ini7it te4r5=t 5? 4ram25sitie mir57este 2resene 5? s8r5se a5=s ?5r te

ierentiati5n 5? t5se i7ri5s, =ian 8tiie s8r5se =it te ai 5?7r5mtym5 78e, an tym5 78e

iniat5rs& e i4 '&6 2H 5? :B"A4ar s822resses 5ter intestina C5ra=ie a5=in4 8nini7ite 4r5=t 5?

i7ri5s&


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M.DIUM LO#.%".I% *.%".%

K55ni 2aa Meia LQ=enstein*ensen akan tam2ak2ert8m78an aam =akt83- min448 7er=arna 28tikek8nin4k8nin4an, 2erm8kaankerin4, kasar Etiak iin an

ra28 en4an te2i yan4 tiak7erat8ran Ese2erti 78n4a k5&e2i @ 7er4eri4i

60


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K5m25sisi Meia LQ=enstein*ensen as2ara4in, KH!PO-,M4

sitrat, M4 s8?at, 4iserin 28ti te8r ayam>7e7ek

maakit ia8 steri !

61

M.DIUM LO#.%".I% *.%".%


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M.DIUM RA%"POR

S#!ar# mei!m@ i2akai 8nt8k trans25rtasis2eimen yan4 men4an8n4 7akteri 25siti?

Gram an ne4ati? Gram& Carr$ a" Blair mei!m@ i2akai 8nt8k

trans25rtasi tina ata8 a28san ret8m yan4i8ri4ai men4an8n4 Vibrioata8

Enterobacteriaceaeyan4 2at54en&


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Mei8m enriment 8nt8k 7akterienterik@ Air e"#," alkali': 8nt8k Vibrio s2eies

aam tina Sele"i#e: Salmonella anShigella s2eies

aam tina&

Mei!m ile e#,": 8nt8k Salmonella

typhiaam ara


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Air 2ent5n akais

:arry an Bair Mei8m

"t8art Mei8m O< Bie"eenite


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KEPEKAAN ANTIBIOTIK

Lak8kan2en48k8ran

iameter 5naerni i sekitaris anti7i5tikE5na ini7isi an

7anin4kan ta7er88kan 8nt8kmenent8kaninter2retasinya&

80


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88


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.RIMA KA"IH


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"IAPR."PO% SSSSSSS


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R."PO% A

ULI" %AMA, %IM ,

K.LOMPOK DA%A"I".%


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1

A& A2a it8 7akteriTB& "e78tkan str8kt8r ari 7akteri T

Emin )


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!

A

B


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3

A& "e78tkan rea4enrea4en yan4i48nakan 2aa 2e=arnaan taanasamT

B& Inter2retasi ariBA ; @BA @


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-

A& A2a ?8n4si ari M5rantTB& A2a 2er7eaan met5e /ie J

%eesen an ani 2aa 2e=arnaan

taan asamTT


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)

A& A2a nama tes7i5kimia

isam2in4TB& A2a J a2a saa

yan4 a2at iiat

2aa tes 7i5kimiaterse78tT


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KUMPUL SSSSSSS


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R."PO% B

ULI" %AMA, %IM ,

K.LOMPOK DA%A"I".%


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1

".BUKA% 3 P.RB.DAA% BAK.RIDA% *AMUR T


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!

A& Pe=arnaan

a2a yan4isam2in4T

B& A2a J a2a at

=arna yan4i48nakan T


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3

A& "e78tkan rea4enrea4en yan4i48nakan 2aa 2e=arnaan 4ram T

B& Inter2retasi ari

Gram ; @Gram @


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-

"e78tkan ! minima 7akteri yan4taan

asamT


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)

a. Apa nama tes

biokimia

disamping ?

b. Apa apa saja

yang dapat dilihat

pada tes biokimiatersebut?


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KUMPUL SSSSSSS

Date post:	01-Mar-2018
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