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Booklet BIO - Attract SPE DISKv3 - AFFINISEP · proposed with a large variety of sorbents (HLB,...

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Order on www.affinisep.com Spinnable, Automatable High throughput HTS Micro-SPE for Microextraction Proteomics, Biomarker discoveries and Biological applications 1 AttractSPE™Disks
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  • Order onwww.affinisep.com

    Spinnable, AutomatableHigh throughput HTS

    Micro-SPE for Microextraction

    Proteomics, Biomarker discoveries and Biological applications

    1

    AttractSPE™Disks

  • Order onwww.affinisep.com 2

  • Order onwww.affinisep.com

    PRODUCT SELECTION GUIDE

    3

    Sampling and sample preparation are the key steps in trace analysis foranalytical chemist. As specialist in this field, AFFINISEP supplies a completerange of solutions based on Solid Phase Extraction (SPE), passive samplingand filtration processes for automated, high-throughput or manual samplepreparation.

    A multitude of chemical phases and formats are available for variousapplications. We offer a comprehensive range of SPE to give you all elementsto face the increasingly complex and diverse sample preparation challengesby:§ Simplify data analysis by removing interferences§ Increase sensitivity and reliability by enrichment of the analyte§ Obtain high and reproducible recovery yields from complex samples

    Solid Phase Extraction SPE Disks Spin SPE

    SPE TipsPassive

    samplers (POCIS, SPATT)

    96 well Plates SPE

    Filtration columns

    Dispersive SPE, Quechers SLE

    SPE Manifolds and

    Accessories

  • Order onwww.affinisep.com

    Solid Phase Extraction Solutions

    4

    2L - 500mL

    • AttractSPE™ Disk

    • Passive samplers: POCIS, SPATT,

    Chemcatcher

    200mL – 1mL

    • AFFINIMIP® SPE

    • AttractSPE™

    • SilactSPE™

    • Qcleanup™ Quechers

    • AttractFiltra™ Filtration

    • AttractSPE™ SLE

    1000µl - 10µl

    • AttractSPE™ Disks Tips – Stagetips

    • AttractSPE™ Disks 96 well-plate SPE

    • AttractSPE™ Disks Spin SPE

    • AttractSPE™ Disks SPE cartridges

    Samplevolume

  • Order onwww.affinisep.com 5

    SPE PROCEDURE STEPS

    1- Sample preparation: This step is necessary to obtain a loading solutioncompatible with retention condition in the SPE column. In case of solid matrices,this solution must also extract the compounds of interest from these matrices.2- Conditioning: The SPE cartridges are conditioned with the appropriated solventsto fully soak sorbent and enable further interactions between the analytes and thesorbent.3- Loading: the percolation solution is loaded through the SPE cartridge. Theanalyte must be retained in the column as well as unwanted compounds.4- Washing: interferences and unwanted compounds are washed off by usingappropriate solvents.5- Elution: The desired analyte is extracted from the SPE cartridge.

    Retention Mechanism

    SOLID PHASE EXTRACTION

    •Based on polar-polar or dipole-dipole interactions betweenthe analyte and a non organic phase like silica.Normal phase

    •Based on non polar- non polar interactions and Van derWaals dispersive forces. The sorbent is hydrophobic likepolymeric sorbent modified silica-based sorbent.

    Reversed phase

    •Uses electrostatic interaction between a charged sorbentand the ionic analyte. The sorbent is charged with theopposite charge of the analyte.

    Ion-exchange

    • Interact through reversed phase and ion exchangeretention mechanisms. Available as a polymeric sorbent(AttractSPE™ SAX, WAX, WCX or SCX) or as SilactSPE™.

    Mixed-mode sorbents

    •Highly selective based on forme and interaction of onemolecule or a familly of moleculesImprinted

    Loading sample Washing Elution

    SPE Sorbent

    Conditioning

    Concentratedand cleaned

    sample

  • Order onwww.affinisep.com

    FORMATS

    Open CartridgeFormats: 1mL, 3mL, 6mL, 15mL, 20mL, 60mL With flange or tablessMaterials: Polypropylene,glass (6mL)Frits: Polyethylene, PTFE , Glass fiberSorbents: powder or diskluer compatible

    ReversibleCartridge

    Formats: 0,7mL, 2mLMaterial: PolypropyleneFrit: PolyethyleneLuer compatible

    LRC Cartridge

    Luer compatibleFormats: 10mL

    Material: PolypropyleneFrit: Polyethylene

    Cartridge for AutomatesFormats: 1mL, 3mL, 6mLMaterial: PolypropyleneFrit: Polyethylene

    On-line SPE CartridgeI.D: 2,1 and 4,6mmLength: 20mm

    6

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    AVAILABLE

    Dispersive SPE

    96 Well-plates

    Sorbents: powder or disks

    SPE Tips

    Spin SPE

    Disks

    Passive SamplersI.D: 54mmO.D.: 90mm

    7

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    Solid Phase Extraction Solutions

    8

    1000µl - 10µl

    • AttractSPE™ Disks SPE Tips –

    Stagetips

    • AttractSPE™ Disks Spin SPE

    • AttractSPE™ Disks 96 well-plate SPE

    • AttractSPE™ Disks SPE cartridges

    Proteomics and

    biological

    applications

  • Order onwww.affinisep.com

  • Order onwww.affinisep.com

    AttractSPE™ Disks Technology for microelution

    10

    AttractSPE™Disks are thin, dense, soft and uniform membranes basedchromatography for extraction/separation, purification and concentrationof analyte molecules.Thanks to their unique advantage, AttractSPE™Disks are useful forpurification of Very Small Sample Volumes in Proteomics, Genomics,Metabolomics, Bioanalysis, Biomarker discoveries and Biologicalapplications. They are applied for Spinnable, Automatable, High throughputmicroelution and nano elution.AttractSPE™Disks offer outstanding sample preparation efficiency andreproducibility of results. Since the diffusion distance between particles isminimized, adsorption is more efficient, and extraction can be accomplishedusing very low sorbent mass.These properties are giving to AttractSPE™Disks a significant improvementof mass transfer kinetics compared to traditional packed SPE particles. As amonolith disk, AttractSPE™Disks are self stand and require no frits forimmobilizing the column bed (unlike traditional SPE products) allowing100% recovery of the original sample volume.

    • No need to frits or filters• Reduced dead volume• Small elution volumes• High sample recovery• Reduced time for eluate evaporation• Higher throughput• Channeling effects eliminated• Excellent reproducibility• Concentration of the sample

    AttractSPE™Disk Advantages

  • Order onwww.affinisep.com

    AttractSPE™ Disks Technology for microelution

    11

    AttractSPE™Disks can be used to miniaturize SPE for small operating volume

    of fluid samples. 4 formats are available for microelution. Thanks to the

    use of AttractSPE™Disks for all formats, then the change of format or the

    scale up of the process is easy.

    q AttractSPE™ Disks Tips – Stagetips and AttractSPE™ Disks 96 well-Plate for microelution

    µSPE columns designed by immobilizing auniform disk inside a pipette tips (Stagetips perunit or as 96 well plate)

    q AttractSPE™ Disks Spin ColumnsSPE column designed by immobilizing a uniform

    disk inside a centrifuge SPE tube

    q AttractSPE™ Disks 96 well-Plate96 SPE well plate designed by immobilizing a

    uniform disk

    q AttractSPE™ Disks CartridgesSPE cartridges designed by immobilizing a

    uniform disk

    Capacityof m

    oleculesof interestincreasesCo

    ncen

    trat

    ion

    of m

    olec

    ules

    of in

    tere

    stin

    crea

    ses

  • Order onwww.affinisep.com 12

    AttractSPE™ Disk Tips, Spin, 96 plate and cartridges are tools forproteomic applications (fractionation and desalting ofpeptides/proteins) and for bioanalysis (small molecules cleanup).In order to give the most exhaustive applications, they areproposed with a large variety of sorbents (HLB, RPS, SDB, SCX, SAX,C8, C18…).

    Focus on AttractSPE™ Disks proteomic tools

    Advantages of AttractSPE™ Disk tools

    •Removes interfering contaminants—significantly reduces signalsuppression and improves signal-to-noise ratios and sequencecoverage

    •Simplifies optimization—processing yields high-quality spectra and iseffective for a variety of reverse-phase-compatible contaminates

    •Robust—works with a wide variety of load volumes andconcentrations; no need to reduce sample volume before application

    •Convenient—easy to handle and requires no special equipment toprocess multiple samples simultaneously (unlike tip-driven systemsthat require one sample to be processed at a time)

    •Sensitive—special C18 resins allows excellent recovery percentages,even at low (sub-picomole) sample loads

    A B C

    A: one membraneB: Stacking of several membranes layersC: membrane used as filter for µm beads

    Different combinations of SPE disks for requested applicationsAttractSPE™ Disks sorbents

    •A broad variety of sorbents foreach required applications•Various formats: disks, spins, 96SPE plates, cartridges•One sorbent - several layers forincreased capacity•Several sorbents - stacking forcomplex applications•Disks used as filter forapplication requiring beads

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    AttractSPE™ Disks chemistries

    13

    Sorbents for SPE Disks forbiomolecular applications Compatible with analytical methods

    - C18 end-capped (C18 EC) for themost hydrophobiccompounds/peptides- C18 Not end-capped (C18 NEC) withthe best combination - large spectrumof interaction - capacity- C18 for the wider interactionspectrum (hydrophobic - hydrophilic)with compounds / peptides

    - Desalting of peptides; fractionationof peptides at acidic and neutral pH- Drug extraction in biologicalsamples,

    C8Desalting of large peptides andproteins; Usage as frits to retainbeads in a tip

    Silica Purification of DNA

    C4 Desalting of large peptides andproteins

    SDB a.k.a PS-DVB Fractionation of peptides at basic pH

    HLB: SDB with hydrophilicmoieties

    Fractionation of peptidesExtraction of small molecules (drugs)in biological fluids

    SDB – RPS: Sulfonic modified SDBsorbent

    Desalting of peptides; fractionationof peptides

    SAX : Anion exchange SDB Fractionation of peptides by salt orpH steps

    SCX : Cation exchange SDB Fractionation of peptides by salt orpH steps

  • Order onwww.affinisep.com

    AttractSPE™ Disks Tips – StageTips

    14

    AttractSPE™Disks Tips are spinnable and automatableTips for high throughput useful for peptide desalting,proteomics, small molecules screening and biomarkerdiscoveries and biological applications

    Tip Volume (μL)

    •200•300•500•1000

    Resin Volume (μL)

    •5•10•12•20•40•80•160•200

    Resin:

    •C18 300Å•C18 100Å•C8 100Å•C4 300Å•Strong Anion•Strong Cation•Weak Anion•Weak Cation•HLB•RPS

    Application

    •Desalting•PCR Purifcation kit

    •GenomicPurification

    •Fractionation•PlasmidPurification

    •Bioanalysis…

    To prepare high quality peptidesamples for LC-MS, it is veryimportant to ensure the overallquality of shotgun proteomicsexperiments. Peptide samplescollected after digestion usuallyneed to be cleaned to removesalts, possible gel pieces (for in-gel digested samples) orparticles (for in-solutiondigested samples), whichotherwise will damage the LCswitching valves or clog thecolumns.

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    AttractSPE™Disks Tips – Stage Tips

    15

    Designation Description Reference – 200µl - 96/pkReference –1mL -

    96/pk

    AttractSPE™ DisksTips C18

    C18 membrane, 96/pk Tips-C18.T1.200.96Tips-

    C18.T1.1000.96

    AttractSPE™ DisksTips C18 EC

    End-capped C18 membrane, 96/pk

    Tips-C18EC.T1.200.96

    Tips-C18EC.T1. 1000.96

    AttractSPE™ DisksTips C18 NEC

    Not end-capped C18 membrane, 96/pk

    Tips-C18NEC.T1.200.96

    Tips-C18NEC.T1.1000.9

    6

    AttractSPE™ DisksTips C8

    C8 membrane, 96/pk Tips-C8.T1.200.96 Tips-C8.T1. 1000.96

    AttractSPE™ DisksTips SDB

    PS-DVB membrane, 96/pk

    Tips-DVB.T1.200.96

    Tips-DVB.T1. 1000.96

    AttractSPE™ DisksTips SDB - RPS

    Modified DVB membrane, 96/pk

    Tips-RPS.T1.200.96

    Tips-RPS.T1. 1000.96

    AttractSPE™ DisksTips SAX

    SAX membrane, 96/pk Tips-SAX.T1.200.96Tips-SAX

    .T1. 1000.96

    AttractSPE™ DisksTips SCX

    SCX membrane, 96/pk Tips-SCX.T1.200.96Tips-SCX.T1.

    1000.96

    AttractSPE™ DisksTips C18-SCX

    Stacking C18 & SCX membranes, 96/pk

    Tips-C18-SCX.T1.200.96

    Tips-C18-SCX.T1. 1000.96

    AttractSPE™ DisksTips C18-SCX-C18

    Stacking C18 & SCX &C18 membranes, 96/pk

    Tips-C18-SCX-C18.T1.200.96

    Tips-C18-SCX-C18.T1. 1000.96

    AttractSPE™ DisksTips SDB-SAX

    Stacking PS-DVB & SAX membranes, 96/pk

    Tips-DVB-SAX.T1.200.96

    Tips-DVB-SAX.T1. 1000.96

    AttractSPE™ DisksTips Silica

    Silica membranes, 96/pk Tips-Si.T1.200.96 Tips-Si.T1. 1000.96

    Advantages•Load your sample on AttractSPE™DisksTips for desalting or fractionate peptides/proteins – bioanalysis of small molecules

    •Several sorbents based Stage-tips andstacking

    •Available as 10, 20, 100, 200µL, 1mL

  • Order onwww.affinisep.com

    AttractSPE™Disks 96 plate for microelution

    16

    Designation Description Reference – 1/pk Reference – 1/pk

    AttractSPE™ Disks 96 plate C18 C18 membrane, 1/pk µ96W-C18.T1.1 µ96W-C18.T2.1

    AttractSPE™ Disks 96 plate C18 EC

    End-capped C18 membrane, 1/pk µ96W-C18EC.T1.1 µ96W-C18EC.T2.1

    AttractSPE™ Disks 96 plate C18 NEC

    Not end-capped C18 membrane, 1/pk

    µ96W-C18NEC.T1.1

    µ96W-C18NEC.T2.1

    AttractSPE™ Disks 96 plate C8 C8 membrane, 1/pk µ96W-C8.T1.1 µ96W-C8.T2.1

    AttractSPE™ Disks 96 plate SDB

    PS-DVB membrane, 1/pk µ96W-DVB.T1.1 µ96W-DVB.T2.1

    AttractSPE™ Disks 96 plate SDB – RPS

    Modified DVB membrane, 1/pk µ96W-RPS.T1.1 µ96W-RPS.T2.1

    AttractSPE™ Disks 96 plate SAX SAX membrane, 1/pk µ96W-SAX.T1.1 µ96W-SAX.T2.1

    AttractSPE™ Disks 96 plate SCX SCX membrane, 1/pk µ96W-SCX.T1.1 µ96W-SCX.T2.1

    AttractSPE™ Disks 96 plate WCX WCX membrane, 1/pk µ96W-WCX.T1.1 µ96W-SCX.T2.1

    AttractSPE™ Disks 96 plate C18-SCX

    Stacking C18 & SCX membranes, 1/pk

    µ96W-C18-SCX.T1.1

    µ96W-C18-SCX.T2.1

    AttractSPE™ Disks 96 plate C18-SCX-C18

    Stacking C18 & SCX &C18 membranes,

    1/pk

    µ96W-C18-SCX-C18.T1.1

    µ96W-C18-SCX-C18.T2.1

    AttractSPE™ Disks 96 plate Silica

    Silica membranes, 1/pk µ96W-Si.T1.1 µ96W-Si.T2.1

    AttractSPE™ Disk 96 Plate for microelution is a 96 plate with wells similar to stagetips fora high throughput clean-up requiring microelution volumes.

  • Order onwww.affinisep.com

    AttractSPE™Disks 96 plate – 1mL

    17

    Designation Description Reference – 1/pk

    AttractSPE™ Disks 96 plate C18 C18 membrane, 1/pk 96W-C18.T1.1

    AttractSPE™ Disks 96 plate C18 EC End-capped C18 membrane, 1/pk 96W-C18EC.T1.1

    AttractSPE™ Disks 96 plate C18 NEC

    Not end-capped C18 membrane, 1/pk 96W-C18NEC.T1.1

    AttractSPE™ Disks 96 plate C8 C8 membrane, 1/pk 96W-C8.T1.1

    AttractSPE™ Disks 96 plate SDB PS-DVB membrane, 1/pk 96W-DVB.T1.1

    AttractSPE™ Disks 96 plate SDB –RPS

    Modified DVB membrane, 1/pk 96W-RPS.T1.1

    AttractSPE™ Disks 96 plate SAX SAX membrane, 1/pk 96W-SAX.T1.1

    AttractSPE™ Disks 96 plate SCX SCX membrane, 1/pk 96W-SCX.T1.1

    AttractSPE™ Disks 96 plate C18-SCX

    Stacking C18 & SCX membranes, 1/pk 96W-C18-SCX.T1.1

    AttractSPE™ Disks 96 plate C18-SCX-C18

    Stacking C18 & SCX &C18 membranes, 1/pk

    96W-C18-SCX-C18.T1.1

    AttractSPE™ Disks 96 plate SDB-SAX

    Stacking PS-DVB & SAX membranes, 1/pk 96W-DVB-SAX.T1.1

    AttractSPE™ Disks 96 plate Silica Silica membranes, 1/pk 96W-Si.T1.1

    AttractSPE™ Disk 96 Plate is a plate with 96 SPE wells of 1mL containingimmobilized SPE disks that enables a high throughput clean-up with thesimultaneous preparation of 96 samples. Thanks to this small sorbentamount and a high efficiency, almost 100% of the original sample isrecovered.

    Advantages

    •All sorbents available on catalog or ondemand•Several sorbent weights available.•Easy handling with automates or liquidhandling robots - spinnable•AttractSPE™ Manifold for 96 wellPlateformat or AttractSPE™ Positive pressureManifold for 96 well Plate

  • Order onwww.affinisep.com 18

    AttractSPE™ Disks Spin Column is an SPE column createdby immobilizing a monolithic disk inside amicrocentrifuge SPE tube. Thanks to its self stand, themonolith disk requires no frits for immobilizing thecolumn bed (unlike traditional SPE products), whichallows essentially 100% recovery of the original samplevolume after a couple of brief centrifugations.

    AttractSPE™ Disks Spin Column for microelution

    Conditioning ElutionWashingAdsorption

    Purifiedsample

    Spin tube

    AttractSPE™ Disks

  • Order onwww.affinisep.com

    AttractSPE™Disks Spin Column

    19

    Designation Description Reference micro spin – 96/pkReference mini

    spin – 96/pk

    AttractSPE™ Disks SpinC18 C18 membrane, 96/pk µSpin-C18.T1.96 Spin-C18.T1.96

    AttractSPE™ Disks SpinC18 EC

    End-capped C18 membrane, 96/pk

    µSpin-C18EC.T1.96 Spin-C18EC.T1.96

    AttractSPE™ Disks SpinC18 NEC

    Not end-capped C18 membrane, 96/pk

    µSpin-C18NEC.T1.96

    Spin-C18NEC.T1.96

    AttractSPE™ Disks SpinC8 C8 membrane, 96/pk µSpin-C8.T1.96 Spin-C8.T1.96

    AttractSPE™ Disks SpinSDB

    PS-DVB membrane, 96/pk µSpin-DVB-T1-96 Spin-DVB.T1.96

    AttractSPE™ Disks SpinSDB – RPS

    Modified DVB membrane, 96/pk µSpin-RPS-T1-96 Spin-RPS.T1.96

    AttractSPE™ Disks SpinSAX SAX membrane, 96/pk

    µSpin-SAX-T1-96

    Spin-SAX.T1.96

    AttractSPE™ Disks SpinSCX SCX membrane, 96/pk µSpin-SCX-T1-96 Spin-SCX.T1.96

    AttractSPE™ Disks SpinC18-SCX

    Stacking C18 & SCX membranes, 96/pk

    µSpin-C18-SCX-T1-96

    Spin-C18-SCX.T1.96

    AttractSPE™ Disks SpinC18-SCX-C18

    Stacking C18 & SCX &C18 membranes,

    96/pk

    µSpin-C18-SCX-C18-T1-96

    Spin-C18-SCX-C18.T1.96

    AttractSPE™ Disks SpinSDB-SAX

    Stacking PS-DVB & SAX membranes, 96/pk

    µSpin-DVB-SAX-T1-96

    Spin-DVB-SAX.T1.96

    AttractSPE™ Disks SpinSilica

    Silica membranes, 96/pk µSpin-Si-T1-96 Spin-Si.T1.96

    Reservoirs for AttractSPE™ Disks Spin 25mL- 96/pk Spin-Res-96 Spin-Res-96

    Advantages

    •4 spin size formats: micro and minispin,15mL and 50mL spin tubes

    •Fast and easy extraction process bycentrifugation

    •High throughput purification•Easy scale up of the process

    Mini spinMicro spin

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    AttractSPE™Disks Spin Column

    20

    Designation DescriptionReference 15mL

    spin tube – 50/pkLoading

  • Order onwww.affinisep.com

    AttractSPE™Disks Cartridges

    21

    DesignationReference – 1mL

    – 50/pkReference – 3mL –

    50/pkReference – 6mL –

    50/pk

    AttractSPE™ DisksCartridge C18 CAR1-C18.T1.50 CAR3-C18.T1.50 CAR6-C18.T1.50

    AttractSPE™ DisksCartridge C18 EC

    CAR1-C18EC.T1.50 CAR3-C18EC.T1.50 CAR6-C18EC.T1.50

    AttractSPE™ DisksCartridge C18 NEC

    CAR1-C18NEC.T1.50

    CAR3-C18NEC.T1.50

    CAR6-C18NEC.T1.50

    AttractSPE™ DisksCartridge C8 CAR1-C8.T1.50 CAR3-C8.T1.50 CAR6-C8.T1.50

    AttractSPE™ DisksCartridge SDB CAR1-DVB-T1-50 CAR3-DVB-T1-50 CAR6-DVB.T1.50

    AttractSPE™ DisksCartridge SDB – RPS CAR1-RPS-T1-50 CAR3-RPS-T1-50 CAR6-RPS.T1.50

    AttractSPE™ DisksCartridge SAX

    CAR1-SAX-T1-50

    CAR3-SAX-T1-50

    CAR6-SAX.T1.50

    AttractSPE™ DisksCartridge SCX CAR1-SCX-T1-50 CAR3-SCX-T1-50 CAR6-SCX.T1.50

    AttractSPE™ DisksCartridge C18-SCX

    CAR1-C18-SCX-T1-50

    CAR3-C18-SCX-T1-50

    CAR6-C18-SCX.T1.50

    AttractSPE™ DisksCartridge C18-SCX-C18

    CAR1-C18-SCX-C18-T1-50

    CAR3-C18-SCX-C18-T1-50

    CAR6-C18-SCX-C18.T1.50

    AttractSPE™ DisksCartridges SDB-SAX

    CAR1-DVB-SAX-T1-50

    CAR3-DVB-SAX-T1-50

    CAR6-DVB-SAX.T1.50

    AttractSPE™ Disks 96 Cartridges Silica CAR1-Si-T1-50 CAR3-Si-T1-50 CAR6-Si.T1.50

    Advantages

    •3 and 6mL format•Larger loading volume with a minimalelution volume

    •High extraction capacity•A broad range of sorbents or sorbentcombination

  • Order onwww.affinisep.com

    SPE ACCESSORIES

    Positive Pressure Manifolds

    Vacuum Manifold

    Mini Vap

    Pump

    22

  • Order onwww.affinisep.com 23

    AttractSPE™ Manifolds

    AttractSPE™Positive Pressure Manifold

    is a Solid Phase Extraction Manifold using

    positive pressure to push the liquid through the

    SPE tips, cartridges or the 96-well plates

    simultaneously. This process confers some very

    interesting advantages to this product in term of

    repeatability.

    Description Reference

    24-position with tips MPP-Tips-KIT

    96-position for 96 SPE well plate MPP-96W-KIT

    24-position, 1 mL MPP-1ML-KIT

    24-position, 3 mL MPP-3ML-KIT

    15-position, 6 mL MPP-6ML-KIT

    Manifold unit and kits

  • Order onwww.affinisep.com

    Vacuum Manifold

    ACC-MAN1 Like all chromatography techniques, Use of SPEcartridges needs a precise control of flow ratefor maintaining reproducible extractions. SolidPhase extraction Vacuum Manifold allows you tocontrol the flow and to process up to 12 (12-portversion) or 24 (24-port version) AFFINIMIP® SPEsamples simultaneously, to gain significantlytime during sample preparation steps.

    Mini PUMP ACC-PUMP Mini diaphragm vacuum pump for solid phaseextraction experimentsØ5.5L/minØ~120 torr vacuumØOil-freeØportable

    Vacuum pump trap

    ACC-TRAP SPE Vacuum pump trap kit

    Installed between the manifold and the vacuumpump, it collects all liquids that are aspiratedpreventing contamination of the vacuum pumpwith a capacity of 1L.

    Vacuum SPE Manifold and ACCESSORIES

    24

    AttractSPE™Vacuum Manifold

    very flexible, allows you to control the flow

    and to process up to 12 or 24 samples

    simultaneously, to gain significantly time

    during sample preparation steps.

  • Order onwww.affinisep.com

    SPE ACCESSORIES

    25

    SPE Accessories Designation Definition Reference

    Manifold SPE Vacuum Manifold 12-port model ACC-MAN1

    SPE Adapter & Reservoir kit

    SPE Adapter & Reservoir kit

    Kit of 12 reservoirs 60ml and adapters for use with1,3 & 6 mL cartridges

    ACC-AR1

    Mini-Vap Mini Evaporator / Concentrator

    6 port Mini-VapEvaporator/Concentratorfor use with 1 to 250mL containers

    ACC-VAP1

    Mini PUMP Mini vacuum pumpLaboport diaphragmvacuum mini pump, 5.5L/min

    ACC-PUMP

    Vacuum pumptrap

    SPE Vacuum pumptrap kit 1L trap kit ACC-TRAP

    SPE ACCESSORIES – Product list

    SPE Adapter & Reservoirkit

    ACC-AR1 Tube adapters serve to pile one SPE tube on topof another to provide different selectivities. Alarger empty syringe barrel can be stacked ontop of a smaller SPE tube to act as a larger loadreservoir. Or, they can serve as an adapter forpositive pressure methods (e.g. from a syringeor air/ N2 line).

    Mini-Vap ACC-VAP1 The 6-Port Mini-Vap concentrator/evaporatorprocesses six vials at one time. The Mini-Vapincludes a needle valve for fine metering of airor nitrogen drying gas.

  • AboutAFFINISEP is a worldwide expert in sample preparation applications.AFFINISEP is dedicated to the development of analytical applications in variousfields such as water, biological fluids, food, feed analysis and proteomics with acomplete set of products and services for sample preparation.

    Analytical chemists can find any solution for sample preparation, selectiveextraction and sample clean-up needs in various sectors: food and feedsafety and quality, life science and quality control, clinical diagnosis,environment and doping.In addition, proteomics users can find a complete set of microextractionproducts for protein/peptides fractionation or desalting.

    Brands

    AFFINIMIP SPE™AttractSPE™

    SilactSPE™…

    Applications

    Sample PreparationPassive SamplingFiltrationMicroextraction of peptides/proteins

    Matrices

    Food, Feed, Soil, Oil, Water,

    Biological fluids,

    Proteolytic digestion

    For any order, please, choose one of the following ways:

    v On our website : www.affinisep.comFind all our references and benefit from a FAST and SIMPLE process !

    vBy email: v [email protected]

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    ORDERING INFORMATION

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