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B.Tech (Biotechnolgy) B.Tech (Biotechnolgy) B.Tech (Biotechnolgy) B.Tech (Biotechnolgy) detail detail detail detail Syllabus for Admission Batch 2015Syllabus for Admission Batch 2015Syllabus for Admission Batch 2015Syllabus for Admission Batch 2015----16161616

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First Semester

Theory Practical

Code Course Name Hours/

Week

L/T

Credit

Theory

University

Marks

Internal

Evaluation

Hours/

Week

L/T

Credit

Practical

Marks

BS Mathematics-I 3-1 4 100 50 - - - BS Chemistry/ Physics 3-0 3 100 50 2 1 50 ES Basics of Electronics /

Basic Electrical

Engineering

3-0 3 100 50 2 1 50

ES Mechanics/

Thermodynamics 3-0 3 100 50

ES Programming in ‘c” 3-0 3 100 50 2 2 50 HS English Communication

Skill 3-0 2 100 50 2 1 50

ES Engineering Workshop/

Engineering Drawing 4 2 100

Total 16 18 600 300 18 7 300

Total Marks: 1200

Total Credits: 25

Second Semester

Theory Practical


week

L/T

Credit

Theory

University

marks

Internal

Evaluation

Hours/

Week

L/T

Credit

Practical

Marks

BS Mathematics-II 3-1 4 100 50 - - - BS Chemistry/ Physics 3-0 3 100 50 2 1 50 ES Basics of Electronics /

Basic Electrical

Engineering

3-0 3 100 50 2 1 50

ES Mechanics/

Thermodynamics 3-1 3 100 50

ES Data Structure Using

‘C” 3-0 3 100 50 2 2 50

HS Business

communication 3-0 2 100 50 2 1 50

ES Engineering Workshop/

Engineering Drawing 4 2 100

MC NSS/NCC - - - -

Total 17 18 600 300 14 7 300

Total Marks: 1200

Total Credits: 25


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Second Year Engineering

Third Semester

Theory Practical


week

L/T

Credit

Theory

University

Marks

Internal

Evaluation

Hours/

Week

L/T

Credit

Practical

Marks

PC Biochemistry 3-0 3 100 50 2 1 50 PC Cell Biology 3-0 3 100 50 2 1 50 PC Biostatistics 3-0 3 100 50 2 1 50 PC Microbiology 3-0 3 100 50 2 1 50 PC Upstream Process

Engineering.-I 3-1 4 100 50

HS Engineering Economics/

Organizational Behavior 2-1 3 100 50

Total 19 19 600 300 8 4 200

Total Marks: 1100

Total Credits: 23 Honours Genetics 4 4 100 50

Minor

Specialization

Fourth Semester

Theory Practical


week

L/T

Credit

Theory

University

Marks

Internal

Evaluation

Hours/

Week

L/T

Credit

Practical

Marks

HS Purely Applied

Mathematics for

Specific Branch of

Engineering

3-0 3 100 50

PC Molecular Biology 3-0 3 100 50 2 1 50 PC Upstream Process

Engineering.-II 3-0 3 100 50 2 1 50

PC Bioinstrumentation 3-0 3 100 50 2 1 50 PC Biochemical Reaction

Engineering 3-0 3 100 50 2 1 50



*Skill Project and Hands

on 6 3 100

Total 18 18 600 300 14 7 300

Total Marks: 1200

Total Credits: 25 Honours Biomolecules

And Cell

Signaling

4 4 100 50

Minor

Specialization

*College should conduct at least one NSDC program under this category.


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Third Year Engineering

Fifth Semester

Theory Practical Code Course Name Hours/W

eek

L/T

Credit

Theory

University

Marks

Internal

Evaluation

Hours/

Week

L/T

Credit

Practical

Marks

PC Genetic Engineering 3-0 3 100 50 2 1 50

PC Plant & Animal

Biotechnology

3-0 3 100 50 2 1 50

PC Industrial Microbiology

& Enzyme Technology

3-0 3 100 50 2 1 50

PE Immunology

&Immunotechnology

3-1 4 100 50

OE DBMS/Process

Instrumentation

3-1 4 100 50

PC Advance Lab-I 8 4 200

Total 17 17 500 250 14 7 350

Total Marks: 1100

Total Credits: 24

Honours Genomics,

Proteomics And

Metabolomics

4 4 100 50

Minor

Specialization

Sixth Semester


eek

L/T

Credit

Theory

University

Marks

Internal

Evaluation

Hours/

Week

L/T

Credit

Practical

Marks

PC Bioreactor Design &

Analysis

3-0 3 100 50 2 1 50

PC Bioinfomatics 3-0 3 100 50 2 1 50

PE Stem Cell Engg./

Molecular Modelling &

Drug Designing

3-1 4 100 50

PE Biomaterials/Nanobiote

chnology

3-1 4 100 50

MC &

GS

Environmental Science

& Engineering

3-0 3 100 50

OE Industrial Lecture # 3 1 50

HS Presentation Skill &

Skill for Interview # #

2-0 1 50 4 2 100

MC Yoga 2 1 50

Total 19 18 500 300 13 7 300

Total Marks: 1100

Total Credits: 24

Honours Protein

Engineering And

Structure Analysis

4 4 100 50

Minor

Specializati

on

# To be conducted by the Training & Placement department by inviting experts from the industry. No academician to be

called. Record may be asked by the University for verification. Evaluation to be done by the TPO.# # To be conducted by

the Training & Placement department of the College.


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Final Year Engineering

Seventh Semester


eek

L/T

Credit

Theory

University

Marks

Internal

Evaluation

Hours/

Week

L/T

Credit

Practical

Marks

GS Nano Science & Bio

Technology

3-1 4 100 50

PE Environmental

Biotechnology

3-1 4 100 50

PE Medical &

Pharmaceutical

Biotechnology

3-1 4 100 50

OE Soft Computing */

Other subjects

3-1 4 100 50

PC Advance Lab-II/ Project 8 4 200

Projects on Internet of

Things

8 4 200

Total 16 16 400 200 16 8 400

Total Marks: 1000

Total Credits: 24

Honours Ethics and IPR in

Biotechnology

4 4 100 50

Minor

Specializati

on

*Student can choose from any department but subject must be running in that semester.

Eighth Semester

Training cum Project Evaluation Scheme


week

L/T

Credit

Theory

Total

Marks

Marks

Industrial Training cum

Project/

Entrepreneurship

Training cum Project /

Stratup Training cum

Project

30 20 1000 Evaluation by the

Industry / Training

Organisation

500

Evaluation by the

Institute

(Report & Institute

Viva)

500

Total 30 20 1000 1000 Total Marks:1000 Total Credits:20

Note- Minimum Pass Mark from Industry Evaluation is 300 (i.e. 60%).


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Distribution of Credit Semester wise:

Semester Credit

First 25

Second 25

Third 23

Fourth 25

Fifth 24

Sixth 24

Seventh 24

Eighth 20

-------------------------------------

Total 190

Internal Evaluation Scheme

Attendance & Class Interaction 05 Assignment 05 Surprise Test 05

Quiz 05 Class Test I & II 30

Total 50 Class Test Time(Hrs.): 1

Pass Mark in Internal is 50% of total marks i.e. 25

External Evaluation Scheme

University Semester Examination of 3 Hours duration. Pass mark will be 35% which means students have to score 35 out of 100.

Practical/Sessional Evaluation Scheme

Pass mark will be 50% which means students have to score 25 out of 50. Evaluation Scheme Attendance & Daily Performance -10 Lab Record - 10 Lab Quiz - 05 Final Experiments & Viva – 25 -------------------------------------------------------

Total=50 All Lab examinations are to be completed one week before the end semester examination and marks are to be displayed on the college notice board.


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DETAIL SYLLABUS FROM

III - VIII SEMESTER OF B.TECH. DEGREE PROGRAMME

for

ADMISSION BATCH 2015-16

BRANCH-BIOTECHNOLOGY

B.Tech (Biotechnolgy) B.Tech (Biotechnolgy) B.Tech (Biotechnolgy) B.Tech (Biotechnolgy) detail detail detail detail Syllabus for Admission Batch 2015Syllabus for Admission Batch 2015Syllabus for Admission Batch 2015Syllabus for Admission Batch 2015----16161616 3rd Semester3rd Semester3rd Semester3rd Semester

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Second Year Engineering

Third Semester

Theory Practical


week

L/T

Credit

Theory

University

Marks

Internal

Evaluation

Hours/

Week

L/T

Credit

Practical

Marks

PC Biochemistry 3-0 3 100 50 2 1 50 PC Cell Biology 3-0 3 100 50 2 1 50 PC Biostatistics 3-0 3 100 50 2 1 50 PC Microbiology 3-0 3 100 50 2 1 50 PC Upstream Process

Engineering.-I 3-1 4 100 50



Total 19 19 600 300 8 4 200

Total Marks: 1100

Total Credits: 23

Honours Genetics 4 4 100 50

Minor

Specializati

on


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3RD SEM

BIOCHEMISTRY Module-I

Structure and Function of Carbohydrates: Monosaccharide, Oligosaccharides, Polysaccharides (Starch,

Glycogen, Cellulose), Optical Isomerism

Structure and Function of Lipids: Saturated and Unsaturated Fatty Acids, Triacylglycerols, Phosphoglycerides,

Sphingolipids, Waxes and Sterol

Structure and Function of Proteins: 20 Amino acids, Peptide bond, Hierarchy of protein architecture,

Ramachandran Plot

Structure and Function of Nucleic Acids: DNA, RNA, Double Helix Model of DNA, Denaturation and

Renaturation DNA;

Structure and function of Hormones, Minerals and Vitamins

Module-II

Principle of Bioenergetics: Bioenergetics and Thermodynamics, Phosphoryl group transfer and energy

currency-ATP; Biological Oxidation and reduction reactions

Metabolism-I: Introduction to metabolic processes; Metabolism of Carbohydrates: Glycolysis, TCA Cycle, ETS

and Oxidative Phosphorylation, Gluconeogenesis ,

Metabolism of Lipids: Anabolism (Saturated), Catabolism (α- Oxidation, β-Oxidation) and Energetics of lipid

metabolism;

Metabolism Of Nucleic Acids: Catabolism and anabolism of purine and pyrimidine nucleotides.

Photosynthesis: Light reaction and dark reaction.

Module-III

Metabolism-II: Metabolism of proteins: Biosynthesis of amino acids (role of precursors);

Enzymes: Properties of Enzyme, Classification of Enzymes, Mechanism of enzyme action, Kinetics of enzyme

action, Activation energy, Enzyme Inhibition, Coenzyme

Text Book

1. Principle of Bio-Chemistry – Lehinger, Nelson and Cox

2. Biochemistry of Biochemistry by L. Stryer

3. Fundamentals of Biochemistry – Voet & Voet

4. Biochemistry by Zubay.

5. Biochemistry, C.B.Powar & G.R.Chatwal, Himalaya Publishing House.

6. Biochemistry, Rastogi, Tata McGraw Hill.

7. Fundamental of Biochemistry, Jain and Jain

BIOCHEMISTRY LAB 1. Spectrophotometric/Colorimetric estimation of Protein using Lowry’s Method

2. Spectrophotometric/Colorimetric estimation of carbohydrates

3. Spectrophotometric estimation of DNA using DPA method

4. Spectrophotometric estimation of RNA using Orcinol Method

5. Estimation of iodine Number and Saponification value of fatty acids

6. Separation of Amino acids by Paper Chromatography

7. Separation of Sugars by Thin Layer Chromatography

8. Separation of Proteins by Column Chromatography

9. Assay of Enzyme activity: Protease from bacteria

10. Assay of Enzyme activity: Amylase from Plant tissue & Saliva

11. Determination of Km and Vmax of an enzyme catalyzed reaction.

Books:

Introduction to Practical Biochemistry, Plummer, Tata McGraw Hill


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CELL BIOLOGY Module-I

Structural intricacy & Chemical composition of cells, Organization of Cell (Prokaryotic and Eukaryotic), Cell

Wall & Cell Membrane, Cell Organelles, Endoplasmic reticulum, Nucleus, Cytoskeleton

Module-II

Molecular Organization of Chromosome (Nucleosome concept), Cell Cycle, Cell Divisions- Mitosis and

Meiosis, Programmed cell death. introduction to cell fate and early embryonic patterning in different

organisms (Drosophila), Stem Cell (Embryonic and adult types and characteristics )

Module-III

Membrane transport & trafficking, mechanisms of protein sorting and targeting, intercellular communication

and associated signaling pathways, cell death pathways, Cancer Cell Biology (Cause, Cell Characteristics)

Text Books

1. Theory & Problems in Molecular & Cell Biology, Stansfield, Tata McGraw Hill

2. The Cell Molecular approach, Geoffrey M. Cooper, ASM press Washington D.C. Sinauer Associates, Inc.

3. Principles of Genetics, Robert Tamarin, Tata McGraw Hill

4. Molecular Biology of Cell – Alberts, Garland Science, Taylor & Francis Group.

5. Molecular Biology of The Cell (Fifth edition), 2007 by Bruce Alberts, Alexander Johnson,

Julian Lewis, Martin Raff, Keith Roberts, Peter Walter. Publisher: Garland Publications

6. Molecular Cell Biology (sixth edition), 2008 by Harvey Lodish, Arnold Berk, Chris A. Kaiser,

Monty Krieger, Matthew P. Scott, Anthony Bretscher, Hidde Ploegh, Paul Matsudaira.

Publisher: W H Freeman & Company

7. Essential Cell Biology (Third Edition), 2009 by Bruce Alberts, Dennis Bray, Karen Hopkin,

Alexander Johnson, Julian Lewis, Martin Raff, Keith Roberts, Peter Walter. Publisher: Garland

Science

CELL BIOLOGY LAB. 1. This Exercise focuses on how to develop a working knowledge of the Microscope and its use.

Students should identify the different parts of the Microscope. List and follow recommended

procedures in using and caring for the Microscope.

2. Mitosis and the Cell Cycle in Onion Root-Tip Cells

3. Meiotic cell division in grasshopper testis

4. Cell Counting and viability study

5. To isolate the mitochondria from the given sample

6. To identify the blood cell types in human blood smear.

7. To identify the different types cells present in the leaf cross section.

8. To prepare permanent slides using the given sections like Plant and animal samples.

9. Separation of lymphocytes and granulocytes from blood sample

10. To study cellular fractionation of a homogenized rat liver via a technique called differential

centrifugation.

11. To study the technical principles underlying Scanning Electron Microscopy (SEM).


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MICRO BIOLOGY Module-I

Introduction to Microbial Kingdom- Bacteria, Viruses, Fungi and Yeast; Classical and Modern approaches of

microbial taxonomy; Classification of bacteria, fungi and Viruses; Methods of Microbiology- Culture media,

Sterilization, Establishment of pure culture, Staining of bacteria (Gram’s, Acid Fast, Capsule), Micrometry and

Microscopy(Bright Field, Fluorescence, Phase Contrast and Electron).

Module-II

Microbial growth and metabolism: Pattern of bacterial growth, Growth kinetics, Monod’s Equation,

Synchronous Growth and its Kinetics, Continuous culture and its growth kinetics, Growth inhibitory

substances. Metabolism of carbohydrate in bacteria, Enerdoudorf,s pathway and glyoxalate pathway, Energy

transduction mechanism in bacteria, Cyanobacteria and nitrogen fixation, Anaerobic respiration.

Microbial genetics: Organization of bacterial and viral genome, Plasmids and Episomes, Genetic

recombination in bacteria (Transformation, Conjugation and Transduction), Genetic analysis in bacteria, DNA

repair mechanisms in bacteria, Transposons, Mutation in Microorganisms.

Module-III

Food Microbiology: Microbiology of foods, Types of microbes associated with food spoilage, Food

preservation methods, Food poisioning, Microbiology of Milk and dairy products.

Medical Microbiology: disease causing bacteria, virus and fungi; Antimicrobial agents, Antibiotics,

Disinfectants and Vaccines

Environmental Microbiology: Microbiology of water, Microbiology of Air, Baceriological analysis of water &

water treatment, Microbiology of extreme environments (Halobacteria, Methanogens, Thermofiles),

Microbiology of sewage.

Text Books :

1. Text book of Microbiology by Stanier.

2. Microbiology by Pelczar

3. Brock Biology of micro-organisms

4. Microbiology by Presscott.

5. Microbial Genetics- Freifelder

6. Microbiology and Immunology by B K Patnaik, T.C. Kar, H.N. Thatoi, India-Tech publication. New Delhi

MICRO BIOLOGY LAB

1. Micrometry: calibration of stage and ocular micrometer and measurement of microbial sample.

2. Staining of microbial sample ( Gram’s Staining, Capsule staining, Fungal staining)

3. Media preparation and sterilization (Slant, Stab and Broth culture)

4. Isolation of micro organisms from natural habitats (Air, Water, Soil & Milk)

5. Establishment of pure culture by streak plate and serial dilution method.

6. Study the bacterial growth curve using spectrophotometer and viability assessment.

7. Antibiotic assay and estimation of Zone of inhibition.

8. Chemical assay and MIC determination of antibiotics.

9. Biochemical assay of microorganisms (Starch Hydrolysis, Casein Hydrolysis and IMVIC test).

10. Microscopy: Study of Compound, Phase contrast and Fluorescence Microscopes.


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MOLECULAR BIOLOGY

Module-I

Genome Organization Prokayotes and Eukaryotes, Nuclear genome and Organellar genome, DNA as the

genetic material, Central dogma of molecular biology, Genome complexity, C- value Paradox, Cot curve

analysis, Repetitive DNA, satellite DNA; Gene structure in prokaryotes and Eukaryotes, Cistron, Recon, Muton;

Variants of gene- Split genes, pseudogenes, Overlapping genes and selfish DNA.

DNA Replication: Models of DNA replication, Enzymology of DNA replication, Process of DNA replication

(Initiation, Elongation, Termination), DNA replication at the telomere, Replication of Mitochondrial and

Chloroplast genome, DNA-protein interaction, DNA repair.

Module-II

Transcription: Components of transcription machinery in prokaryotes and eukaryotes, Transcription factors,

Transcription process (Initiation, Elongation, Termination), Transcription factors, m-RNA processing, Pre and

Post transcriptional processing, Capping and poly (A) tailing, m-RNA stability, m-RNA editing, Gene Silencing

Module-III

Translation: Genetic code- the principle of translation, Translation machinery (t-RNA, Aminoacyl synthetase,

Ribosome), Translation process, Post translational modification of protein.

Regulation of Gene Expression: Constitutive and Induced gene expression, Regulation of gene expression in

prokaryotes and eukaryotes, Operon model (Lac-operon and Trp- operon), DNA methylation. Molecular

evolution (DNA based phylogenetic trees and its applications), Introduction to recombinant DNA technology.

Books :

1. Text Book of Molecular Biology By Padmanabhan and Shastri.

2. MOLBIO – Avinash & K. Upadhyaya, Himalaya Publishing House.

3. Mol. Biology. by Turner.

4. Mol. “Biology of Gene” – Watson

5. Principles of Mol. Biology - OS Prim Rose

6. Recombinant DNA Technology – Watson

7. Mol. Cell Biology. - Baltimore

MOLECULAR BIOLOGY LAB 1. Isolation, purification of DNA from plant sample and its yield estimation.

2. Isolation, purification of DNA from blood sample and its quantification using UV spectrophotometer.

3. Isolation, purification of DNA from bacterial sample and its quality assessment using UV

spectrophotometry.

4. Isolation of plasmid DNA and estimation its size using agarose gel electrophoresis.

5. Effect of gel concentration on solidification and migration of DNA sample

6. Restriction digestion of supplied DNA sample and estimate the molecular weight of the fragments

resulted.

7. Elution of the DNA from the supplied gel and assess the integrity of the fragments.

8. Isolation and purification of RNA from plant/bacterial sample and its quantification using UV

spectrophotometer.

9. Isolation and purification of protein from the supplied sample and its quantification using UV

spectrophotometer.


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BIOSTATISTICS Module-I:

Introduction and definition of Biostatistics; Concept of variables in biological systems.

Collection, Classification, tabulation graphical and diagrammatic representation of numerical

data; Measures of central tendency: Mean, Median and Mode and their relationship; Measures of

dispersion: Range, Quartile deviation, Mean deviation, Standard deviation, Concept of standard

error, Coefficient of variation, Skew ness and Kurtosis.

Module-II:

Probability: Random experiment, events, sample space, mutually exclusive events, independent

and dependent events; Various definitions of probability, addition and multiplication theorems

of probability, Random variables (discrete and continuous), Probability density functions and

its properties; Probability distributions: normal, Binomial, Poisson and their application.

Module-III:

Concept of populations and sample. Simple random sampling without replacement. Definition of

simple random sample; Designing of Experiments-Random block design and Split plot design;

Correlation and Regression, linear and quadratic regression; Analysis of variance: One- way and

two-way classifications with single observation per cell. Duncan’s multiple range test; Tests of

significance: Chi-square, student’s t, z and f-distributions, their properties and uses.

Text Books:

1. Biostatistics: Rao KS, Himalaya Publishing House

2. Introduction to Biostatistics & Research Methods: Sundar Rao PSS & Richard J, PHI

learning Pvt. Ltd.

3. Biostatistics: Arora and Mohan, Himalaya Publishing House

BIOSTATISTICS LAB The practicals to be conducted with available sample data set.

1. Introduction to biostatistics and measurement.

2. Construct and interpret graphical displays such as histograms, box plots, bar charts,

stem & leaf plots, and bivariate scatterplots

3. Collection of sample data and opening sample datasets

4. Calculating measures of central tendency and dispersion.

5. Hypothesis testing; Define null and research hypotheses. Practice conducting and

interpreting T-test in Stata and interpreting confidence intervals

6. Hypothesis testing; Define null and research hypotheses. Practice conducting and

interpreting ANOVA and interpreting confidence intervals

7. Conduct and interpret correlation

8. Conduct and interpret linear regression

9. Conduct and interpret multiple linear regression

10. To calculate and interpret binomial probabilities and Normal probabilities


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UPSTREAM PROCESS ENGINEERING-I

Module-I: Fluid Mechanics concept ( 14 lectures)

Physical properties of Fluid: Density, specific gravity, specific weight, specific volume, surface

tension and capillarity, viscosity, compressibility and bulk modulus, fluid classification.

Fluid static pressure, Pascal’s, pressure variation for incompressible fluid, atmospheric

pressure, absolute pressure, gauge pressure and vacuum pressure, manometer.

Hydrostatic pressure on submerged surface, force on a horizontal submerged plane surface,

force on a vertical submerged plane surface.

Buoyancy and flotation, Archimedes’ principle, stability of immersed and floating bodies,

determination of metacentric height.

Module –II: Elements of fluid dynamics ( 12 lectures )

Basic equations of fluid flow: Newtonian and non-Newtonian fluids, turbulence and its nature,

Reynolds number and transition from laminar to turbulent flow, boundary layer flow, continuity

equation, Bernoulli’s equation, pump workin Bernoulli’s equation.

Flow of incompressible fluids: Fluid flow in pipes, friction factor, laminar flow in pipes, Hagen-

Poiseuille equation, effect of roughness, friction factor charts, Reynolds number and friction

factor relationship

Flow through packed beds: Ergun, Kozney- Carman and Blake-Plummer equations, principles of

two-phase and three-phase fluidizaion.

Module-III : Transportation and metering of fluids ( 10 lectures )

Pipe fittings and valves, positive displacement pumps, power and efficiency of pumps,

centrifugal, plunger and piston pumps, blower and compressor, flow measuring devices viz.

venture meter, orifice meter, pitot tube and rotameter. Drag force and terminal settling

velocity, principles of solid-liquid separation- filters and centrifuges.

Text Books

1. McCabe, Smith and Harriot, Unit Operations of Chemical Engineering

2. Foust et al, Principles of Unit Operations.

3. Treybal, R.E. Mass Transfer Operations

4. Badger and Banchero. Introduction to Chemical Engineering.

5. Foust, Wenzel, Clump, Maus and Andersen, Principles of Unit Operations.

6. Geankoplis, Transport processes and unit operations


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GENETICS Module-II Principles of Inheritance: Chromosome theory of Heredity, Mendelism, Non-Mendelian Gene

Interactions (Epistasis, Lethality, Pleiotropy), Allelic Complementation, Cytoplasmic

Inheritance,

Module-II Linkage and Crossing over, Chromosome mapping, Mutation and Chromosomal Aberration,

Transposable genetic elements, Heterosis and Hybrid vigour, Inbreeding depressions, Genetic

diseases in Human ( Colour blindness, Haemophillia).

Module-III Quantitative Inheritance: Polygenes and Multiple alleles, Introduction to QTLs and its

inheritance, Detection of QTLs

Principles breeding in plants and animals

Population Genetics: Hardy-Weinberg’s law, Genetic Equilibrium, Changes in gene frequency,

Genetic Drift, Effect of evolutionary forces on genetic equilibrium of apopulation.

Developmental genetic with reference to Arabidopsis and Drosophilla.

Text Books

1. Theory & Problems in Molecular & Cell Biology, Stansfield, Tata McGraw Hill

2. The Cell Molecular approach, Geoffrey M. Cooper, ASM press Washington D.C. Sinauer

Associates, Inc.

3. Principles of Genetics, Robert Tamarin, Tata McGraw Hill

4. Molecular Biology of Cell – Alberts, Garland Science, Taylor & Francis Group.

5. Concept of Genetics by Klug.

6. Genetics, David R Hyde, Tata McGraw Hill.


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ENGINEERING ECONOMICS Theory L/T (Hours per week):2/1, Credit: 3

Module I (12 hours) Engineering Economics- Nature, Scope, Basic problems of an economy, Micro Economics and

Macro Economics.

Demand- Meaning of demand, Demand function, Law of Demand and its exceptions,

Determinants of demand, Elasticity of demand & its measurement (Simple numerical problems

to be solved ), Supply-Meaning of supply, Law of supply and its exception, Determinants of

supply, Elasticity of supply, Determination of market equilibrium (Simple numerical problems

to be solved).

Production-Production function, Laws of returns: Law of variable proportion, Law of returns to

scale

Module II (12 hours) Cost and revenue concepts, Basic understanding of different market structures, Determination

of equilibrium price under perfect competition (Simple numerical problems to be solved), Break

Even Analysis-linear approach (Simple numerical problems to be solved).

Banking -Commercial bank, Functions of commercial bank, Central bank, Functions of Central

Bank.

Inflation-Meaning of inflation, types, causes, measures to control inflation.

National Income-Definition, Concepts of national income, Method of measuring national income.

Module III (12 hours) Time value of money- Interest - Simple and compound, nominal and effective rate of interest,

Cash flow diagrams, Principles of economic equivalence.

Evaluation of engineering projects-Present worth method, Future worth method, Annual worth

method, Internal rate of return method, Cost benefit analysis for public projects .

Depreciation- Depreciation of capital assert, Causes of depreciation, Methods of calculating

depreciation (Straight line method, Declining balance method), After tax comparison of project.

Text Books

1. Riggs, Bedworth and Randhwa, “Engineering Economics”, McGraw Hill Education

India

2. Principles of Economics, Deviga Vengedasalam; Karunagaran Madhavan, Oxford

University Press.

3. Engineering Economy by William G.Sullivan, Elin M.Wicks, C. Patric Koelling,

Pearson

4. R.Paneer Seelvan, “ Engineering Economics”, PHI

5. Ahuja,H.L., “Principles of Micro Economics” , S.Chand & Company Ltd

6. Jhingan,M.L., “Macro Economic Theory”

7. Macro Economics by S.P.Gupta, TMH


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ORGANIZATIONAL BEHAVIOUR

Credit- 3 Class Hours - 40

Objectives:

1. To develop an understanding of the behavior of individuals and groups inside

organizations

2. To enhance skills in understanding and appreciating individuals, interpersonal,

and group process for increased effectiveness both within and outside of

organizations.

3. To develop theoretical and practical insights and problem-solving capabilities for

effectively managing the organizational processes.

Unit Contents Class

Hours

01 Fundamentals of OB: Definition, scope and importance of OB,

Relationship between OB and the individual, Evolution of OB,

Theoretical framework (cognitive), behavioristic and social cognitive),

Limitations of OB.

6

02

Attitude: Importance of attitude in an organization, Right Attitude,

Components of attitude, Relationship between behavior and attitude,

Developing Emotional intelligence at the workplace, Job attitude,

Barriers to changing attitudes.

Personality and values: Definition and importance of Personality for

performance, The Myers-Briggs Type Indicator and The Big Five

personality model, Significant personality traits suitable to the

workplace (personality and job – fit theory), Personality Tests and their

practical applications.

Perception: Meaning and concept of perception, Factors influencing

perception, Selective perception, Attribution theory, Perceptual process,

Social perception (stereotyping and halo effect).

Motivation: Definition & Concept of Motive & Motivation, The Content

Theories of Motivation (Maslow’s Need Hierarchy & Herzberg’s Two

Factor model Theory), The Process Theories (Vroom’s expectancy

Theory & Porter Lawler model), Contemporary Theories – Equity

Theory of Work Motivation.

10

03 Foundations of Group Behavior: The Meaning of Group & Group

behavior & Group Dynamics, Types of Groups, The Five – Stage Model of

Group Development.

Managing Teams: Why Work Teams, Work Teams in Organization,

Developing Work Teams, Team Effectiveness & Team Building.

Leadership: Concept of Leadership, Styles of Leadership, Trait

Approach Contingency Leadership Approach, Contemporary leadership,

Meaning and significance of contemporary leadership, Concept of

transformations leadership, Contemporary theories of leadership,

Success stories of today’s Global and Indian leaders.

9


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04 Organizational Culture : Meaning & Definition of Organizational

Culture, creating & Sustaining Organizational Culture, Types of Culture

(Strong vs. Weak Culture, Soft Vs. Hard Culture & Formal vs. Informal

Culture), Creating Positive Organizational Culture, Concept of

Workplace Spirituality.

8

05 Organizational Change: Meaning, Definition & Nature of

Organizational Change, Types of Organizational Change, Forces that acts

as stimulants to change.

Implementing Organizational Change : How to overcome the

Resistance to Change, Approaches to managing Organizational Change,

Kurt Lewin’s-Three step model, Seven Stage model of Change & Kotter’s

Eight-Step plan for Implementing Change, Leading the Change Process,

Facilitating Change, Dealing with Individual & Group Resistance,

Intervention Strategies for Facilitating Organizational Change, Methods

of Implementing Organizational Change, Developing a Learning

Organization.

7

Reference Books

1. Understanding Organizational Behaviour, Parek, Oxford

2. Organizational Behaviour, Robbins, Judge, Sanghi, Pearson.

3. Organizational Behaviour, K. Awathappa,HPH.

4. Organizational Behaviour, VSP Rao, Excel

5. Introduction to Organizational Behaviour, Moorhead, Griffin, Cengage.

6. Organizational Behaviour, Hitt, Miller, Colella, Wiley

B.Tech (Biotechnolgy) B.Tech (Biotechnolgy) B.Tech (Biotechnolgy) B.Tech (Biotechnolgy) detail detail detail detail Syllabus for Admission Batch 2015Syllabus for Admission Batch 2015Syllabus for Admission Batch 2015Syllabus for Admission Batch 2015----16161616 4th4th4th4th SemesterSemesterSemesterSemester

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Fourth Semester

Theory Practical


week

L/T

Credit

Theory

University

Marks

Internal

Evaluation

Hours/

Week

L/T

Credit

Practical

Marks

HS Purely Applied

Mathematics for

Specific Branch of

Engineering

3-0 3 100 50

PC Molecular Biology 3-0 3 100 50 2 1 50 PC Upstream Process

Engineering.-II 3-0 3 100 50 2 1 50

PC Bioinstrumentation 3-0 3 100 50 2 1 50 PC Biochemical Reaction

Engineering 3-0 3 100 50 2 1 50



*Skill Project and Hands

on 6 3 100

Total 18 18 600 300 14 7 300

Total Marks: 1200

Total Credits: 25 Honours Biomolecules

And Cell

Signaling

4 4 100 50

Minor

Specialization


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9

4TH SEM

MOLECULAR BIOLOGY Module-I Genome Organization Prokayotes and Eukaryotes, Nuclear genome and Organellar genome,

DNA as the genetic material, Central dogma of molecular biology, Genome complexity, C- value

Paradox, Cot curve analysis, Gene structure in prokaryotes and Eukaryotes, Split genes,

pseudogenes, Overlapping genes and selfish DNA

Module-II DNA Replication: Models of DNA replication, Enzymology of DNA replication, Process of DNA

replication (Initiation, Elongation, Termination), DNA replication at the telomere, Replication of

Mitochondrial and Chloroplast genome, DNA-protein interaction, Mutagenesis, DNA repair,

Regulation of DNA Replication.

Module-III Transcription: Components of transcription machinery in prokaryotes and eukaryotes,

Transcription process (Initiation, Elongation, Termination), Post transcriptional processing, m-

RNA editing, Gene Silencing Translation: Genetic code, Translation machinery (t-RNA,

Aminoacyl synthetase, Ribosome), Translation process, Post translational modification of

protein.

Regulation of Gene Expression: Constitutive and Induced gene expression, Regulation of gene

expression in prokaryotes and eukaryotes, Operon model (Lac-operon and Trp- operon), DNA

methylation.

Books : 1. David Friefelder - Molecular Biology, 2nd Ed., Norasa Publishing Home 1987.

2. Lodish & Baltimore, Molecular Cell Biology, 5th Ed., W.H. Freeman & Company, 2003.

3. Gerald Karp - Cell and Molecular Biology, Concepts andExperiments, 5 th Edition, John

Wiley and Sons Pvt. Ltd., 2008.

4. Benjamin Lewin - Gene – VIII, 1 st Edition,2004

5. Channarayappa - Molecular BiotechnologyPrinciples and Practices, 1 st Edition, 2006.

University Press.

UPSTREAM PROCESS ENGINEERING-II Module-I:Processing of particulates Properties and handling of particulate solids, size reduction equipments- working principles of

crusher, grinder and pulverizer, screening and particle size distribution.

Module-II : Heat Transfer concepts and applications Heat transfer and drop wise condensation, Heat transfer to boiling liquid, working principles of

different types of heat transfer equipments like heat exchanger, condenser, evaporator and

autoclave.

Module-III: Mass transfer concepts and applications Diffusion concepts: adsorption equillibria., working principles of mass transfer equipments like

adsorber, absorber, distillation column and humidifier.

Text Books

1. McCabe, Smith and Harriot, Unit Operations of Chemical Engineering

2. Foust et al, Principles of Unit Operations.

3. Treybal, R.E. Mass Transfer Operations

4. Badger and Banchero. Introduction to Chemical Engineering.

5. Foust, Wenzel, Clump, Maus and Andersen, Principles of Unit Operations.


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BIOINSTRUMENTATION Module – I Spectroscopic methods to study physicochemical properties of Biomolecules: UV-Vis, IR, CD &

ORD, DSC, FTIR Fluorescence & phosphorescence. Electrophoresis: General principle of

electrophoresis, support media (agarose and polyacrylamide gels), electrophoresis of proteins

by SDS-PAGE, native PAGE, gradient gels, isoelectric focusing, two dimensional PAGE, Blotting

Techniques: Western, Southern, Northern blot analysis, visualization of proteins in gels,

electrophoresis of nucleic acids using agarose gel.

Module – II Brief introduction to NMR spectroscopy, hydrogen-deuterium exchange Mass spectroscopy and

X-ray crystallography and their application. Hydrodynamic properties–viscosity. Principles of

electron microscopy, preparation of samples, TEM, SEM

Radioisotope Techniques: Radioactivity activity detection methods based on ionization (Geiger-

Muller monitor), excitation (solid and liquid scintillation counting), Cerenkov counting,

autoradiography, safety aspects of handling radioactive material

Module – III Chromatography: Principles of chromatography, distribution coefficient, retention time,

capacity factor, plate height and resolution, peak broadening and van Deemter plot, TLC and

column chromatography, matrix materials, LPLC, HPLC, normal phase and reversed phase

chromatography, ion exchange chromatography, gel exclusion chromatography, affinity

chromatography, Gas Chromatography

Biosensors: Immobilization key to biosensor construction, Types of Biosensor, whole cell

biosensors. Applications and uses of biosensors, Clinical chemistry, medicine and health care,

Veterinary, Agriculture and Food production, Environmental control and pollution monitoring.

Books: 1. Principles and Techniques of Biochemistry and Molecular Biology, Wilson K. and Walker

J., Cambridge University Press (2005) 6th ed.

2. Biochemical Method-A Concise guide for students and researchers, Pingoud A., Urbanke

C., Hoggett J. and Jeltsch A. Wiley-VCH Publishers

3. Bioseparations: Science and Engineering, Harrison, R.G., Todd, P., Rudge, S.R. and

Petrides, B.B. Oxford University Press (2006).

4. Molecular Spectroscopy, McHale, J.L., Prentice Hall (1998).

5. Microscopy and Microtechniques. Marimuthu, R., MJP Publishers (2008).

6. Instrument Methods of Analysis, Willard H.W., Merritt L.L., Dean J.A. & Settle F.A. 6th ed.

East West Publishers,

7. Turner, A.P.F., Karube.I.,and Wilson, G.S. Biosensors Fundamentals and applications,

Oxford Univ.Press.


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BIOCHEMICAL REACTION ENGINEERING Module I: Material, energy balance and concepts of reaction engineering Material Balance & Energy Balances: Mathematical requisites – use of log-log and semi-log

graph paper, triangular diagram, graphical differentiation and graphical integration, material

balance without chemical reaction, material balance with chemical reaction, energy balance;

enthalpy changes, heat of reaction and its temperature dependence, heats of solution and

mixing, adiabatic flame temperature, use of psychometric charts. Kinetics of homogeneous

reactions: classification of reactions, reaction rate, speed of reaction, rate equation,

concentration-dependent term of rate equation, rate constant, order and molecularity,

representation of elementary and nonelementary reactions, kinetic models for nonelementary

reactions, temperature-dependent term of a rate equation, activation energy and temperature

dependency.

Module II: Reaction engineering of batch and continuous reactors Kinetic analysis of batch reactor data: Integral and differential methods for analyzing kinetic

data, interpretation of constant volume batch reactor, data for zero, first, second and third order

reactions, half life period, irreversible reaction in parallel and series, auto catalytic reaction.

Kinetic interpretation of batch reactor data for single reactions: interpretation of variable

volume batch reaction data for zero, first and second order reactions, Ideal batch reactor, steady

state CSTR and plug flow reactors and their use for kinetic interpretation. Design for single

reaction: size comparison of single reactors, plug flow reaction in series and/or parallel, equal

and different size of mixed reactor in series, finding the best system for given conversion,

recycle reactor, Design of multiple reactions in batch, CSTR and PFR.

Module III: Heterogeneous reactions and Biochemical reactions Reaction catalyzed by solids: introduction to heterogeneous reactions, rate equation for surface

kinetics, pore diffusion resistance combined with surface kinetics, porous catalyst particles,

performance equations for reactors containing porous catalyst particles, experimental methods

for finding rates, advantages and disadvantages of packed bed and fluidized bed catalytic

reactors. Biochemical reaction systems: microbial fermentation, batch fermentor and mixed

flow fermentor, kinetic expressions of fermentation.

Books 1. Chemical process Principles (Part I & II), Houge, Watson & Ragatz, Asian Student

Edition, Asia Publishing House.

2. Basic Principles and Calculations in Chemical Engineering, Himmelbalu, Prentice Hall (l)

6th Ed.

3. Coulson & Richardson’s Chemical Engineering- Volume 3 (Chemical and Biochemical

Reactors and process controls) ed. Ricchardson. J.F., Peacock. D.G.,First Indian ed. Asian

Books Pvt.Ltd. 1998.

4. Levenspiel, O., Chemical Reaction Engineering,Wiley Eastern Ltd.

5. Smith & Vanness, Thermodynamics for Chemical Engineers, MGH.

6. Bailey & Oils, Biochemical Engg. Fundamentals, MGH

7. Foggler, Elements of Chemical Reaction Engineering


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MOLECULAR BIOLOGY LAB 1. Isolation, purification of DNA from plant sample and its yield estimation.

2. Isolation, purification of DNA from blood sample and its quantification using UV

spectrophotometer.

3. Isolation, purification of DNA from bacterial sample and its quality assessment using UV

spectrophotometry.

4. Isolation of plasmid DNA and estimation its size using agarose gel electrophoresis.

5. Effect of gel concentration on solidification and migration of DNA sample

6. Restriction digestion of supplied DNA sample and estimate the molecular weight of the

fragments resulted.

7. Elution of the DNA from the supplied gel and assess the integrity of the fragments.

8. Isolation and purification of RNA from plant/bacterial sample and its quantification

using UV spectrophotometer..

9. Isolation and purification of protein from the supplied sample and its quantification

using UV spectrophotometer.

UPSTREAM PROCESS ENGG LAB 1. Experiments on Reynold’s Apparatus-Determination of flow regime and construction of

friction factor against NRe.

2. Experiments on flow measuring devices - in closed conduit using (a) Venturimeter, (b)

Orifice meter (c) Rotameter.

3. Study and verification of conservation of energy of a flowing liquid in a Bernoulli’s

apparatus.

4. Determination of Pressure drop for flow through packed bed & verification of Ergun

Equation, Kozeny-Karman equation, Blake-Plummer Equation.

5. To Determine the Overall heat transfer coefficient of a concentric pipe heat exchanger

based on the inside diameter of the tube.

6. To calculate the heat loss in a lagged pipe made of various insulating materials.

7. Determination of volumetric mass transfer coefficient (Kla) of gas-liquid system.

8. Determination of mixing time in stirred tank reactor.

9. To determine the coefficient of absorption/adsorption in packed bed columns.

10. To separate the solute from one phase to another (aqueous to solvent) phase by liquid-

liquid extraction.

11. Double Pipe Heat Exchanger.

12. Shell and Tube Heat Exchanger.


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BIOINSTRUMENTATION LAB 1. UV-Visible spectroscopy: UV – spectrophotometric analysis of DNA and protein

samples/ Determine λmax of DNA, protein, bromophenol blue solutions by wavelength

scan

2. Determination of secondary structure of protein by Circular dichroism (CD)

spectroscopy.

3. Denaturation of proteins and nucleic acids.

4. Determination of Protein- ligand interaction.

5. Column chromatographic analysis of chlorophyll

6. 2D-TLC analysis of amino acids

7. Use of viscometer in protein analysis

8. Comparison of Coomassie brilliant blue and silver staining methods for visualizing

protein bands in SDS-PAGE

9. Comparison of ethidium bromide and silver staining methods for visualisation of small

DNA fragments analyzed by native PAGE

10. Fluorescence spectroscopy

11. GC & HPLC (demonstration)

12. Differential scanning calorimetry (DSC)

13. FTIR

14. Electron microscopy (SEM/TEM)

BIOCHEMICAL REACTION ENGINEERING LAB 1. Use of log-log and semi-log graph paper, triangular diagram, graphical differentiation

and graphical integration

2. Kinetic Studies in C.S.T.R

3. Kinetic Studies in P.F.R

4. Kinetic Studies in Combined Reactor

5. Kinetic Studies in Batch Reactor

6. Adiabatic Batch Reactor

7. R.T.D Studies in C.S.T.R

8. R T D Studies in C.S.T.R’s in Series R.T.D Studies in Plug Flow Reactor

9. R.T.D Studies in Combined Reactor.

TEXT BOOKS:

1. Octave Levenspiel - Chemical Reaction Engineering , 3rd Ed. John Wiely & Sons,1999.

2. H.S. Fogler - Elements of Chemical Reaction Engineering, 2nd ed. PHI, 1992.


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HONORS: BIOLOGICAL MACROMOLECULES AND INTERACTION Module-1 Introduction to biological macromolecules: General principles, Molecular interactions in

macromolecular structures. Equilibrium binding: Boltzmann distributions and energy levels.

Ligand-protein interactions, protein-protein interactions. Binding polynomials and partition

functions; linked functions; coupled equilibriums. Phenomenological binding models vs.

rigorous models of allosteric regulation and site-site cooperativity. Practical considerations of

binding analysis; nonlinear least squares analysis. ITC, DSC and SPR methods .

Module-II Nucleic acids: Special properties of nucleic acids; counterion condensation theory; effect of salt

concentration and type on nucleic acid structure and nucleic acid-protein interactions. RNA

structure and folding. Single-molecule nucleic acid biophysics.

Module-III Single molecule science: Mechanisms of protein-ligand (nucleic acid) and protein-protein

interactions; dynamics of intramolecular structural changes; single-molecule FRET; single-

molecule force spectroscopy monitored by laser optical tweezers and atomic force microscopy.

Books 1. Thermodynamic Theory of Site-Specific Binding Processes in Biological Macromolecules,

DiCera (Cambridge University Press, 1995);

2. Molecular Driving Forces:Statistical Thermodynamics in Chemistry and Biology, Dill and

Bromberg (Garland Sciences, 2003).

Suggested Readings / Books: 1. Molecular Biophysics by Daune, M, Oxford University Press (1999).

2. Biophysics by Glaser, R, Springer (2004).

3. Lehninger’s Principles of Biochemistry by Nelson, D.L., Cox, M.M., McMillan Publishers

(2008) 4th ed.

B.Tech (Biotechnolgy) B.Tech (Biotechnolgy) B.Tech (Biotechnolgy) B.Tech (Biotechnolgy) detail detail detail detail Syllabus for Admission Batch 2015Syllabus for Admission Batch 2015Syllabus for Admission Batch 2015Syllabus for Admission Batch 2015----16161616 5th Semester5th Semester5th Semester5th Semester

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5

Fifth Semester

Theory Practical


Week

L/T

Credit

Theory

University

Marks

Internal

Evaluation

Hours/

Week

L/T

Credit

Practical

Marks

PC Genetic Engineering 3-0 3 100 50 2 1 50 PC Plant & Animal

Biotechnology 3-0 3 100 50 2 1 50

PC Industrial Microbiology

& Enzyme Technology 3-0 3 100 50 2 1 50

PE Immunology

&Immunotechnology 3-1 4 100 50

OE DBMS/Process

Instrumentation 3-1 4 100 50

PC Advance Lab-I 8 4 200

Total 17 17 500 250 14 7 350

Total Marks: 1100

Total Credits: 24 Honours Genomics,

Proteomics

And

Metabolomics

4 4 100 50

Minor

Specialization


Pa

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6

5TH SEM

GENETIC ENGINEERING Module-I Basic principle of DNA isolation and purification; Restriction endonuclease, Ligase and other

modifying enzymes; DNA& RNA Markers, Linker, Adapter and MCS; Gene cloning vectors-

Plasmid, bacteriophage, cosmid, BAC, YAC; Expression vectors: basic concept, bacteria and yeast

based expression vector; Gene library- genomic and c-DNA, contig library; Polymerase Chain

reaction,

Module-II Basic concept of gene cloning; Cloning of interacting gene: two hybrid and three hybrid assay;

Cloning of differentially expressed gene; DNA micro arrays and Chips - principle and

manufacturing process; DNA finger printing and DNA foot printing; DNA Sequencing; Site

directed mutagenesis; Expression of heterologous gene; In vitro transcription and translation;

Gene knock out strategies; RNA interference: Antisense RNA, si RNA and mi RNA; Ribozyme

Technology.

Module-III Molecular markers- Types (RFLP, RAPD, AFLP, SCAR, SSR, SNP, EST), Principle and

methodology; Application of molecular markers: in diagnostics, gene tagging, gene mapping,

Physical mapping of the genome. Genome analysis using 16S rRNA typing/ sequencing, Genome

projects: Human, Rice; Gene therapy and its applications; DNA vaccines and rDNA products;

Genetic engineering regulations and safety guidelines.

Text Books: 1. H S Chawla, Plant Biotechnology, Oxford University Press

2. Genetic Engineering, Smita Rastogi & Neelam Pathak, Oxford University Press

3. T A Brown, Gene cloning and DNA Analysis, Black well publishing

4. Primerose et al., Principle of gene manipulation and genomics, Black well publishing.

Reference

1. Sambrooks et al., Molecular cloning (Vol.I,II,II), CSH Press.

2. Spurr N & Young TD, ICRF Hand book of genome analysis, Blackwell


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PLANT AND ANIMAL BIOTECHNOLOGY Module-I: Plant Biotechnology Concept of totipotency and plasticity of plant cell, Tissue culure media- preparation,

composition and plant growth regulators, Initiation and establishment of culture: Explant

preparation, Callus culture, Single cell culture, Suspension culture, Microspore culture

Micropropagation: Organogenesis, Somatic embryogenesis, Artificial seed; Protoplast

technology: Isolation and culture of protoplast, Somatic hybridization, Screening and selection

of somatic hybrid.

Secondary metabolites of plant origin and its type; Production of secondary metabolites through

tissue culture, Factors affecting the production and its optimization, Bioreactor based

production of secondary metabolites and its kinetic studies, isolation and purification of

secondary metabolites, Biotransformation with case studies.

Module-II: Animal Biotechnology Equipments and materials for animal cell, culture technology, Brief discussion on the chemical,

physical and metabolic functions of different constituents of culture medium, Development of

primary culture. Development of cell line by enzymatic disaggregation, Culture media and

growth conditions. Cell growth characteristics and kinetics,

Measurement of viability and cytotoxicity; Biology and characterization of the cultured cells,

measuring parameters of growth

Module-III: Techniques and applications Direct transformation (Gene gun, Electroporation, Microinjection, etc.) Concept of genetic

transformation: Vector based (Agrobacterium, Virus) and

Application of genetic transformation: promoter tagging, activation tagging, herbicide

resistance, insect resistance, disease resistance, molecular farming, terminator seed technology;

Products of genetic transformation: Case studies for golden rice, Bt cotton and Flavr Savr

tomato.

Cell transformation, Application of animal cell culture, stem cell cultures, embryonic stem cells

and their applications. Hybridoma technology, Organ culture technology, Transfection of animal

cells, Future tissue engineering, animal cloning

Text Books: H S Chawla, Plant Biotechnology, Oxford University Press.

K G Ramawat, Plant Biotechnology, S. Chand & Co.

A Kumar and SK Sopory, Recent advances in Plant Biotechnology, I.K. International.

A Slater et al., Plant Biotechnology, Oxford Univ. Press

Text Books:

1. R. Ian Freshney, Culture of Animal Cells, 3rd Edition, Wiley-Liss publication

2. Martin Clynes, (Eds) Animal Cell culture Techniques Springer Publication

3. Balasubramanian, Bryce, Dharmalingam, Green and Jayaraman (Eds.),Concepts in

Biotechnology, University Press, 1996.

4. Hood L.E., Weissman I., Wood W.B. & Wilson J.H. Immunology,Benjamin Cummings,

1989.

5. Biotol Series – Butterworth and Heineman, Oxford, 1992

6. A Text Book of Biotechnology R C Dubey, S Chand publication

7. Animal cell culture by R.I. Freshney

8. Animal Biotechnology by P.Ramadas

9. In vitro cultivation of Animal cells by Dr.C.K.Leach,Butterworth and Heinnmamm

Ltd.1994.

10. Hand book of Animal Husbandry by Gopalakrishnan .


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INDUSTRIAL MICROBIOLOGY AND ENZYME TECHNOLOGY Module-I: Microbial Processes and fermentation technology: Introduction to fermentation technology,

Microbial growth and product formation kinetics in batch, continuous and feed batch

fermentation, Large scale production: submerged, solid and semi-solid fermentation,

Microbiological processes for production of organic acids; solvents; antibiotics, enzymes,

polysaccharides; lipids; pigments and aroma.

Module-II: Commercial media and strain development: Media selection and development for industrial

production, Isolation, selection, characterization of microorganisms, stock culture, development

inocula, strain improvement: induced mutation, over producing decontrolled mutants,

genetically engineered strain and fermentation.

Module-III: Stability of enzyme: Enzyme stabilization by selection and genetic engineering, protein

engineering. Application of enzymes in industry, analytical purpose and medical therapy.

Application of Biocatalyst, Group transfer redox, Elimination, isomarization and rearrengement,

C-C bond cleavage, Reaction environment rebuilding, chemical modification, intramolecular

cross linking and immobilization.

Text Books 1. Principle of Fermentation Technology , P.F. Stanbury, A. Whitaker and S.J. Hall, Elsevier

2. Industrial Microbilogy, Prescot and Dunn,

3. Biochemical Engineering and Biotechnology Handbook, Atkinson, B and Marituna, F.,

The Nature Press, Macmillan Publ. Ltd.

4. Biochemical Engineering Fundamentals, Bailey & Olis. MGH.

IMMUNOLOGY AND IMMUNOTECHNOLOGY Module-I: The origin of Immunology, types of immunity, humoral and cell mediated immunity, Primary

and secondary lymphoid organ, antigen, cells of immune system, immunoglobulin and

antibodies, Major Histocompatibility Complex (MHC)

Module-II: Antigen processing and presentation, synthesis of antibody and secretion, Molecular basis of

Immunology, Molecular basis of antibody diversity, polyclonal and monoclonal antibody,

complement system, antigen-antibody reaction.

Module-III Immune response and tolerance: Regulation of immune response, immune tolerance, hyper

sensitivity, autoimmunity; graft versus host reaction, Immuno- deficiency and immuno-

proliferate diseases. Dysfunctions of immune system and their modulation, Approaches for

correcting immune dysfunction, Vaccinology, Monoclonal antibody technology.

Text Books: 1. Immunology: Lydyard, P.M., Whelan, A., Fanger, M.W., 1st Ed., Viva Books.

2. Essential Immunology: Roitt, I.M., 9th Ed.(1997) Blackwell Scientific, Oxford, UK.

3. Immunology: Kuby, J. 3rd Ed. (1997) Freeman W. H., oxford.

4. Immunotechnology by A Khan, Pearson Publication

Reference:

1. Immunology and Immunotechnology, Ashim K. Chakravarty, Oxford University Press

2. Microbiology and Immunology by B K Patnaik, T.C. Kar, H.N. Thatoi, India-Tech

publication. New Delhi


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GENETIC ENGINEERING LAB 1. Isolation and Restriction enzyme digestion of bacterial genomic DNA

2. Purification of digested DNA-column purification

3. Preparation of target DNA by linker/adapters/alkaline phosphatase treatment for cloning

4. Ligation of DNA fragment with cloning vector

5. Preparation of competent cells

6. Transformation in E.coli with recombinant vector

7.Isolation of recombinants and confirmation of insert DNA in vector

8. Preparation of DNA probe by nick translation /PCR

9. Amplification of DNA sample by PCR

1. Southern Hybridisation

2. Western Hybridisation

3. Expression of cloned gene.

4. DNA profiling by RAPD

PLANT AND ANIMAL BIOTECHNOLOGY LAB

PLANT BIOTECHNOLOGY 1. Media preparation, sterilization, explant preparation and establishment of meristem

culture

2. Study of organogenesis and multiple shoot generation

3. Somatic embryogenesis in carrot and encapsulation somatic embryo or PLBs

4. Anther culture of Datura

5. Establishment of suspension culture

6. Agrobacterium mediated transformation (Co-cultivation & GUS expression)-

7. Embry/Endosperm Culture

8. Isolation of protoplasts

ANIMAL BIOTECHNOLOGY 1. Preparation of culture media and sterilization

2. Organ culture. Fibroblast culture.

3. Adaptation of Animal virus in cell lines BHK-21-vero cell line.

4. Study of effect of anti cancer agent in cell culture.

5. MTT Assay

6. Live cell counting

7. Leukocyte culture

8. Culturing of spleen cells

9. Myeloma cell culture

10. Fusion of cells by PEG

INDUSTRIAL MICROBIOLOGY AND ENZYME TECHNOLOGY LAB

INDUSTRIAL MICROBIOLOGY 1. Isolation of high yielding microbial strains for the production of commercially important

enzymes.

2. . Partial purification of isolated enzymes.

3. Preparation of starter culture of yeast in grape juice

4. Submerged fermentation in single-culture, co-culture condition

5. Production and optimization microbial metabolites (Ethanol or Citric acid) and study of

fermentation kinetics

6. Analysis of alcohol or citric acid by spectrophotometric method and gravimetric method.

7. Fermenter operation and study of product formation by batch culture.


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ENZYME TECHNOLOGY LABORATORY: 1. Enzyme assay techniques (e.g Alkalne phosphatase / Amylase / Invertase /

dehydrogenases) using whole cells and/ or purified enzyme; Kinetic studies and

determination of Km and Vmax

2. Effect of inhibitors on enzyme kinetics

3. Immobilization of cells and enzymes using different matrices (PVA, alginate, etc.)

4. Application of immobilized cells and enzymes in batch and continuous stirred tank

reactors.

5. Biotransformation reaction using whole cell and/or enzyme(s).

6. Formulation of enzyme stability.

7. Method of checking the purity of the enzyme -SDS-PAGE

SE GENETIC ENGINEERING AND PLANT BIOTECHNOLOGY LAB 1. Media preparation and sterilization

2. Callus propagation of medicinal plants and woody plants.

3. Cell suspension culture

4. Isolation and purification of active compounds from plants by column chromatography

technique

5. Electroporation

6. Agrobacterium mediated transformation

7. Developing RFLP maps

8. Southern hybridization.

9. Strain improvement techniques- physical, chemical and genetic manipulation methods.

HONORS: GENOMICS, PROTEOMICS AND METABOLOMICS Module-I: Introduction to genomics: Orientation and structure of genomes, subdividing the genome,

assembling a physical map of a genome. Sequencing methods and strategies, genome annotation

and information from web, bioinformatics

Genome sequencing projects- Microbes, plants and animals; Accessing and retrieving genome

project Reverse genetics, Structural genomics, Functional genomics and Comparative genomics;

High throughput screening in genome for drug discovery-identification of gene targets,

Pharmaco-genomics and drug development.

Module-II: Mapping protein interaction and applications: Global expression profiling, comprehensive

mutant libraries, mapping protein interactions, applications of genome analysis and genomes.

Introduction and tools of proteomics: Proteomics and Proteomes, Various tools used in

proteomics (N-terminal sequencing of proteins, 2-D electrophoresis Differential display

proteomics, Yeast two hybrid and three hybrid system, phage display, isoelectrofocusing,

Peptide fingerprinting. LC/MS-MS for identification of proteins and modified proteins, SAGE,

Protein micro array). Applications of proteomics: Mining proteomes, protein expression

profiling, identifying protein – protein Interactions and protein complexes, mapping- protein

identification, new directions in proteomics, structural proteomics; Proteomics and Drug

delivery. Transcriptomics.

Module-III: Metabolite isolation and analysis by Mass Spectrometry, Sample preparation (fractionation,

enrichment, derivatization), metabolite library, Profiling based on NMR, LIF, LC-UV, 2-D and

high (spatial) resolution metabolite profiling, Quantitative metabolomics Metabolite

analysis and biochemical pathways: Carbon pathway, Secondary metabolism, amino acid

metabolism, Engineered metabolism, Systems biology: Databases (Metabolic pathways

resources) and pathway reconstruction.


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Texts / References Book: 1. Voet D, Voet JG & Pratt CW, Fundamentals of Biochemistry, 2nd Edition. Wiley

2. Brown TA, Genomes, 3rd Edition. Garland Science

3. Campbell AM & Heyer LJ, Discovering Genomics, Proteomics and Bioinformatics, 2nd

Edition. Benjamin Cummings

4. Glick BR & Pasternak JJ, Molecular Biotechnology, 3rd Edition, ASM Press

5. Pennington SR & Dunn MJ, Proteomics, Viva publications

6. H.D.Kumar, Molecular Biology, 2nd edition, Vikas Publishing House Pvt. Lt.

7. Singer, M, and Berg.P – Genes and genomes, Blackwell Scientific

Publication,Oxford,1991.

8. Beebe.T, and Burke. T,Gene Structure and Transcription, 2nd edition, 1992, Oxford Univ

Press.

9. Introduction to Proteomics by Daniel. C. Liebler, Humana press, 2002,198 pages.

10. Principles of gene manipulation and genomics by Primrose, S.B. and Twyman, R.M.,

Blackwell Publishing (2006)

11. Introduction to Genomics by Lesk AM, Oxford University Press (2008)

12. Proteomics: from protein sequence to function by Pennington, S.R. and Dunn, M. J., Viva

Books (2001)


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Sixth Semester


eek

L/T

Credit

Theory

University

Marks

Internal

Evaluation

Hours/

Week

L/T

Credit

Practical

Marks

PC Bioreactor Design &

Analysis

3-0 3 100 50 2 1 50

PC Bioinfomatics 3-0 3 100 50 2 1 50

PE Stem Cell Engg./

Molecular Modelling &

Drug Designing

3-1 4 100 50

PE Biomaterials/Nanobiote

chnology

3-1 4 100 50

MC &

GS

Environmental Science

& Engineering

3-0 3 100 50

OE Industrial Lecture # 3 1 50

HS Presentation Skill &

Skill for Interview # #

2-0 1 50 4 2 100

MC Yoga 2 1 50

Total 19 18 500 300 13 7 300

Total Marks: 1100

Total Credits: 24

Honours Protein

Engineering And

Structure Analysis

4 4 100 50

Minor

Specializati

on


Pa

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3

6TH SEM

BIOREACTOR DESIGN & ANALYSIS Module- I: Principles and concepts Recapitulation of the principles of Kinetics for chemical and Bio-chemical Reactions.

Fundamentals of homogeneous reactions for batch / semi-batch, plug low reactor (PFR),

continuous stirred rank reactors (CSTR), fluidized bed reactor bubble column, air lift fermenter

etc, stirred tank/mixed reactors., adiabatic and programmed reactors. Unconventional

bioreactors: Hollow fiber reactor, membrance reactor, perfusion reactor for animal and plant

cell culture.

Module- II: Bioreactor Analysis Analysis of ideal bioreactors: Fed-Batch reactors, Enzyme catalyzed reactions in CSTRs,

CSTR reactors with Recycle and wall growth, Ideal Plug-Flow Tubular reactor. Analysis of Non-

ideal Reactor Analysis: Concept of ideal and non-ideal reactor; residence time distribution;

models of non-ideal reactors – plug flow reactor for microbial processes; Mass transfer in

biochemical processes; Multiphase bioreactors – packed bed with immobilized enzymes or

microbial cells; three – phase fuidized bed trickling bed reactor; Design and analysis of the

above reactor systems; Gas liquid reactors, Reactor stability.

Module- III: Bioreactor Design Design considerations: oxygen transfer, heat transfer, rheology, mixing. Scale up and scale down

concepts. Bioprocess control and computer coupled bioreactors; Growth and product formation

by recombinant cells. Mechanical fittings in a bioreactor: vessel, agitation system materials,

welds, finish, valves, piping and valves for biotechnology. Instrumentation and control of

bioprocesses: Bioreactor sensor, online sensors for cell properties, off-line analytical methods;

Biosensors. Bioreactor design calculation.

Text Books 1. Levenspiel, O., Chemical Reaction Engineering, Wiley Eastern Ltd.

2. Bailey & Olis, Biochemical Engg. Fudamentals, MGH.,1990

3. Atkinson, B.,Biological Reactors, pion Ltd., London,1974.

4. Coulson, Richardson, Sinnott, An introduction to chemical engineering design, Pergamon

5. Alba S., Humphrey E and Milli N.R., “Bio Chemical Engineering” Academic Press, 1973.

6. Scragg.A.H “Bioreactors in Biotechnology”- A Practical approach

7. Bailey and Ollis, “Biochemical Engineering Fundamentals”, McGraw Hill (2nd Ed.). 1986.

Press.

8. Lydersen, D’Elia, Nelson, Bioprocess engineering: Systems and equipment.


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BIOINFORMATICS Module-I:

Searching Biological Data From databases: Finding the information stored and its retrieval

methods at NCBI, EMBL and DDBJ, Protein Data Bank, CSD, Uniprot, PIR, SwissProt, TrEMBL,

SNP, Metabolic pathways databases KEGG, MetaCyc, Domain classification databases: SCOP,

CATH, Pfam Retrieving microbial and viral genome information.

Module-II: Sequence alignment and analysis: Local and global alignment, Gap penalty and substitution

matrix, Pairwise and Multiple sequence alignment, Fast Alignment method, dynamic

programming, Sequence profile and HMM, Basic algorithms for prediction of ORF, promoters,

splice sites

Module-III: Molecular modelling and drug design: Homology modelling, Molecular mechanics and force

fields, molecular dynamics simulation, Drug design Process, drug like Property of a molecule,

target identification, Docking methods, Basic idea about Molecular descriptors and QSAR

analysis.

Text Books:

1. Mount DW, Bioinformatics: Sequence and Genome Analysis, Spring Harbor Press

2. Arthur Lesk, Introduction to Bioinformatics, Oxford University Press.

3. Baxevanis AS and Ouellette BF, Bioinformatics: A Practical Guide to the Analysis of

Genes and Proteins, Wiley International Science.

4. Bryan Bergeron, Bioinformatics computing, Prentice Hall Inc

5. Bernhard houbold ,Thomas Wiehe,Introduction to computational biology : an

evolutionary approach Blkhauser verlag press

Reference: 1. Tao Jiang, Ying Xu, Michael Q. Zhang, Current Topics in Computational Molecular

Biology, MIT press.

2. Thomas lengauer,Bioinformatics from genome to drug .WILLEY-VCH press.


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STEM CELL ENGG./ MOLECULAR MODELING AND DRUG DESIGNING

STEM CELL ENGG Module-1 Stem cell basics: Unique properties of stem cells, embryonic stem cells, adult stem cells,

umbilical cord stem cells, similarities and differences between embryonic and adult stem cells.

Properties of stem cells, pluripotency, totipotency. Embryonic stemcell: In vitro fertilization,

culturing of embryos-isolation of human embryonic stem cells, blastocyst, inner cell mass,

growing ES cells in lab, laboratory tests to identify ES cells, stimulation ES cells for

differentiation, properties of ES cells.

Module-II Adult stem cells: Somatic stem cells, test for identification of adult stem cells, adult stem cell

differentiation, trans differentiation, plasticity, different types of adult stem cells. Stem cell in

drug discovery and tissue engieering: Target identification, Manipulating differentiation

pathways, stem cell therapy Vs cell protection, stem cell in cellular assays for screening stem

cell techniques: fluorescence activated cell sorting (FACS), time lapse video, green fluorescent

protein tagging, stem cell based drug discovery, drug screening and toxicology.

Module-III Genetic engineering and therapeutic application of stem cells: Gene therapy, genetically

engineered stem cells, stem cells and Animal cloning, transgenic animals and stem cells,

Therapeutic applications Parkinson disease, Neurological disorder, limb amputation, heart

disease, spinal cord injuries, diabetes, burns, HLA typing, Alzheimer’s disease, tissue

engineering application – production of complete organ, kidney, eyes, heart, brain. Stem cell

regulations, debate, social and ethical concerns

Books 1. Embryonic Stem cells by Kursad and Turksen. 2002.Humana Press.

2. Stem cell and future of regenerative medicine. By committee on the Biological and

Biomedical applications of Stem cell Research.2002.National Academic press

3. Hematopietic Stem Cell Transplantation by Treleaven, J., first edition 2009

4. Essentials of Stem Cell Biology by Lanza, R., second Edition, 2009 Academic Press

5. Molecular Cell Biology by Lodish et al., sixth Ed., W.H. Freeman & Co. 2008

6. Stem Cells: From Bench to Bedside by Bongso and Ariff BTBT903 Nanobi


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MOLECULAR MODELING AND DRUG DESIGNING Module-I: Introduction to Molecular Modelling and its applications Biomolecular modeling problems:

protein folding, protein misfolding, nucleic acid/ protein interactions, and RNA folding. Basic

concepts of quantum mechanics, ab initio, semi-empirical and density functional theory

calculations, Molecular size versus accuracy. Approximate molecular orbital theories. Molecular

mechanisms, energy calculations, Bond stretch, Angle bending, torsional terms, Electrostatic

interaction- Van der waals interactions. Miscellaneous interactions.

Module-II: Introductionto molecular dynamics and simulations; Molecular Dynamics using simple models;

Dynamics with continuous potentials, Constant temperature and constant dynamics;

Conformation searching and systematic search; Monte-carlo simulation of biomolecules and

bio-polymers.

Comparative modeling of protein: by homology- the alignment, construction of frame work,

selecting variable regions, side chain placement and refinement, validation of protein models –

Ramchandran plot, threading and ab initio modeling.

Module-III: Analog based drug designing : Introduction to QSAR. lead module, linear and nonlinear modeled

equations, biological activities, physicochemical parameter and molecular descriptors,

molecular modelling in drug discovery.

Structure based drug designing: 3D pharmacophores, molecular docking, De novo Ligand

design, Free energies and solvation, electrostatic and non-electrostatic contribution to free

energies. 3D data base searching and virtual screening, Sources of data, molecular similarity

and disimilarity searching, combinatorial libraries – generation and utility.

Text Book: 1. A R Leach, Principles and applications of modeling, Prentice Hall.

2. Hans Pieter, Heltje & Gerd Folkens, Molecular Modelling, VCH.

References:

1. Jonathan Good man, Chemical Applications of Molecular Modelling, Cambridge Press


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BIOMATERIALS / NANOBIOTECHNOLOGY

Biomaterial

Module-I: Introduction to biomaterials, Structure and properties (mechanical, thermal, optical, electrical

and surface) of biomaterials, Synthetic polymer, Biopolymer

Module-II Novel Biomaterials and uses in engineering and tissue engineering: Hydrogels, self-assembling

peptides. Implants materials: metallic implant materials, stainless steels, Co-based alloys, Ti-

based alloys; ceramic implant materials, aluminum oxides, hydroxyapatite glass ceramics

carbons. Polymeric implant, Polymers for drug delivery: types of polymer, pharmaceutical

polymers, physicochemical properties of polymers and relationship with structure, properties,

kinetics, mechanisms and applications.

Module- III: Biocompatibility and blood compatibility, Biomaterials: its foreign body response in a body.

Biological interface, interaction with biomaterials and adhesion, Biological response to

implants, 2D and 3D matrices (scaffolds) of biomaterials for tissue engineering, Soft tissue and

hard tissue replacement, cardiovascular implants, Biomaterials for ophthalmology, orthopaedic

and dental implants, Biologically functional biomaterials, Testing and clearance of biomaterials,

Evaluation of biomaterials.

Text Books 1. Buddy D. Ratner Allan S. Hoffman Frederick J. Schoen Jack E. Lemons. Biomaterials

Science, Second Edition: Wiley Science 2004.

2. Bhatt SV, Biomaterial, Narosa publishing house

3. Park J and R. S. Lakes R S, Biomaterials: An Introduction, Springer 2009


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NANOBIOTECHNOLOGY

Module-I Concept of Nano-biotechnology & Historical background. Fundamental sciences and broad areas

of Nanobiotechnology, Various applications of Nano-biotechnology, biology at the nano

interface, Cell – Nanostructure interactions, Science of Self-assembly and self organization from,

Module-II Structural and functional principles of nanobiotechnology. Protein-based Nanostructures:

Nanomotors and DNA based nanostructures. Tools of measurement: Scanning probe techniques

(SPM), Electron Microscopy. Introduction to Nanostructures: Fullerenes, Quantum Dots and

Metal-based Nanostructures, nanopolymers. Carbon nanotubes, nanoparticles and nanowires.

Microbial synthesis of nanoparticles, magnetosomes, bacteriorhodopsin. Micro- and nano –

fabrication: Chemical Vapor Deposition, Photolithography, Features of Nanoscale Growth,

Micromachining: MEMS; BioMEMS, microarray technology,

Module-III Microfabricated devices, Nanoanalysis and nanobiosensors; Lab-on-a-chip devices and their

potential in nanobiotechnology. Medical Applications of Nanobiotechnology: Polymeric

nanocontainers for drug delivery and gene delivery, imaging applications, Nanoparticles’

Cytotoxicity

Text Book: 1. Niemeyer C M and Mirkin C A, Nanobiotechnology: Concepts, Applications and

Perspectives, Wiley VCH, 2004

2. Chattopadhyaya KK and Banerjee AN, Nanoscience and Nanotechnology, PHI learning

Pvt. Ltd.

3. Bionanotechnology by David S.Goodsell, 2004, Wiley Publications. Pages-337.


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BIOPROCESS ENGG/ /COMPUTATIONAL BIOLOGY/ STRUCTURAL

BIOLOGY

BIOPROCESS ENGG Module-1 Introduction to bioprocess: Historical development of bioprocess technologies, role of

bioprocess engineer in the biotechnology industry, concept of Bioprocess, outline of an

integrated bioprocess and the various (upstream and downstream) unit operations involved in

bioprocesses, generalized process flow sheets. A brief survey of organisms, processes, products

and market economics relating to modern industrial biotechnology. Fermentation process:

General requirements of fermentation processes; Isolation, preservation and improvement of

industrially important microorganisms, development of innocula for industrial fermentations.

Different types of fermentations, Basic design and construction of fermentor and ancillaries, An

overview of aerobic and anaerobic fermentation processes and their application in the

biotechnology industry solid-substrate fermentation and its applications.

Module-II Metabolic stoichiometry and energetics: Stoichiometry of cell growth and product formation,

elemental balances, degrees of reduction of substrate and biomass available, electron balances,

yield coefficient of biomass and product formation, maintenance coefficients, energetics

analysis of microbial growth and product formation, oxygen consumption and heat evolution in

aerobic cultures, thermodynamic efficiency of growth. Media design and sterilization for

fermentation process: Designing of media for fermentation processes, Types of media, design

and usage of various commercial media for industrial fermentations, thermal death kinetics of

micro organisms, batch and continuous heat sterilization of liquid media, filter sterilization of

liquid media, air, design of sterilization equipment.

Module-III Kinetics of microbial growth and product formation: Phases of cell growth in batch cultures,

simple unstructured kinetic models for microbial growth, Monod model, growth of filamentous

organisms. Growth associated (primary) and non-growth associated (secondary) product

formation kinetics, Leudking – Piret models, substrate and product inhibition on cell growth

and product formation.

REFERENCE BOOKS:

1. Pauline.M.Doran ., “Bioprocess Engineering Principles”;Academic press .

2. Peter F.Stanbury, Allan Whitaker, “Principles of Fermentation Technology”

3. Michael L.Shuler and Fikret Kargi, “Bioprocess Engineering Basic concepts”, Prentice

Hall, 1992.


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COMPUTATION BIOLOGY Module-I Introduction to Computational Biology: Introduction, Biomolecular sequence analysis – Nucleic

acid sequences, Motifs – localization and extraction, Protein sequence analysis and prediction of

secondary structural features. Discrete Models of Biopolymers: Discretized structure models –

Lattice proteins, contact graphs. Combinatorial considerations – secondary structure graphs.

Random graph models of sequence structure maps, RNA secondary structures.

Module-II Protein Structure Folding & Prediction and DNA- Protein Interaction: Overview of protein

structure, Protein folding invitro and invivo, Theoretical models of folding, Insilico folding,

Protein structure prediction - Alignment based methods. DNAProtein Interaction – Target

prediction, sequence based methods, Structure based method, Ab inito method.

Module-III Computational Genomics: Sequences and contigs, Sequence data description, Advanced

Sequence data description,Genome annotation- Eukaryotic and Prokaryotic genome annotation

tools. Computer simulated functions.

Computation in Comparative Genomics: Introduction, Evolutionary basis, Tools for comparative

genomics – data selection, Alignment, Visualization .

Text Books: 1. Andrezej K Konopka and James C. Crabbe, Compact Handbook - Computational Biology,

Marcel Dekker, USA, 2004.

2. Peter Clote, Rolf Backofen - Computational Molecular Biology An Introduction, John Wiley &

Sons ltd.1997.

3. SC Rastogi, N. Mendiratta. P.Rastogi-Bioinformatics Methods and Applications:

Genomics,Proteomics and Drug Discovery, Prentice Hall India Publications, 2005.

Reference Books: 1. David W. Mount - Sequence and Genome Analysis, Published by CSHL Press Science,

2004.

2. S. Salzberg, D. Searls,and S. Kasif - Computational Methods in Molecular Biology, Elsevier

Science, 1998.

3. Joao Setubal and Joao Meidanis - Introduction to Computational Molecular Biology

Publisher: PWS Publishing Company, Boston, 1997.


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STRUCTURAL BIOLOGY Module-1 Structures of biological macromolecules: Levels of structures in proteins, nucleic acids and

polysaccharides - primary, secondary, tertiary and quaternary structures. Conformational

analysis of proteins: protein structure: Polypeptide chain geometries, internal rotation angles,

Ramachandran plot, potential energy calculations, forces that determine protein structure –

hydrogen bonding, hydrophobic interactions, ionic interactions, disulphide bonds – prediction

of protein structure.

Module-II Conformational analysis of nucleic acids: General characteristics of nucleic acid structure –

geometric – Glycosidic bond – rotational isomers, ribose puckering – backbone rotation angles

and steric hindrances – forces stabilizing ordered forms – base pairing and base stacking

Module-III Techniques for the study of biological structure: Electron Microscopy, Ultracentrifuge,

Viscometry, Molecular –sieve chromatography, electrophoresis, NMR and EPR. Other

techniques: X-Ray crystallography, X-ray fiber diffraction, light scattering, Neutron scattering

Books 1. Biophysical Chemistry, Cantor and Schimmel, part I and II, W.H. Freeman and co 1997.

BIOREACTOR DESIGN & ANALYSIS LAB (UNDER REVISION) 1. Bioreactor operation – Demonstration

2. Batch, fed batch and continuous cultures a) Estimation of Monod parameters b) Pure

and mixed cultures.

3. Temperature effect on growth-estimation of energy of activation and Arrhenius constant

for micro-organisms.

4. Determination of Oxygen transfer rate

KLa determination by sulphite oxidation method

KLa determination by dynamic gassing method

KLa determination by power correlation analysis

5. Packed bed bioreactor: study of process parameters

6. Fluidised bed reactor: study of process parameters

7. Screening of process variables single dimensional search, Blackett Burman design,

design expert etc.

8. Study of rheology of fermentation broth and power determination.

9. Bioprocess control using software

10. Production of secondary metabolites by feed batch culture.


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BIOINFORMATICS LAB 1. Retrieving Human genome data, OMIM, SNP databases to understand genetic and

metabolic disorders. (At least 2 each)

2. Mining genomic data to identify genomic features: codon usage, repeats, Homologous

sequences etc.

3. Making Phylogenetic tree of given sequences by using ClustalW and PHYLIP.

4. Gene and promoter prediction for Prokaryotes and eukaryotes (comparative analysis by

using different tools: at least 3)

5. Learning about molecule visualisation software like Rasmol, Pymol etc.

6. Primary Structural databases: pdb, ndb, csd and Derived databases of structures: DSSP,

FSSP, CATH & SCOP.

7. Prediction of secondary structures of proteins: at least 3 methods

8. Prediction of Tertiary structure of proteins and Validation of model protein structure:

Energy minimization, Procheck, verify 3D, Prosa II, ERRAT etc.

9. Molecule drawing. Conversion of 2D structure to 3D structure.

10. Molecular docking and analysis of receptor with ligand

11. Molecular Dynamics simulation

HONORS: PROTEIN ENGINEERING AND ANALYSIS Module- I Overview of protein structure and its hierarchical architecture; Protein engineering – definition,

applications; Forces stabilizing proteins – Van der waals, electrostatic, hydrogen bonding and

weakly polar interactions, hydrophobic effects. Structural features of protein, Ramachandran

map, Protein-protein, Protein-DNA, protein-ligand interactions. Protein structure-function

relationship.

Stability of Protein Structure: Laws of thermodynamics, heat, energy and work, chemical

equilibrium flexibility, reversible folding and unfolding, pH titration, chemical denaturation,

thermal denaturation, solvent perturbation and chemical modification,

Module-II Features or characteristics of proteins that can be engineered- affinity and specificity;

Experimental methods of protein engineering: Rational designing, Directed evolution like site

directed mutagenesis, Module shuffling, Guided protein recombination, etc.; Computational

approaches to protein engineering. Mechanism of stabilization of proteins from psychrophiles

and thermophiles vis-à-vis those from mesophiles; Protein and enzyme engineering case studies

for its stability, specifity and affinity- Protease, Lipase and Lysozyme. Role of solvent.

Module-III Characterization of proteins: NMR spectroscopy, crystallography, spectroscopic (UV-Vis, CD, IR,

Florescence), calorimetric methods, Viscometry, Molecular sieve chromatography,

electrophoresis, EPR in protein structure and function analysis with example.

Text Books /References: 1. Edited by T E Creighton, Protein structure: A practical approach, 2nd Edition, Oxford

press.

2. Edited by T E Creighton, Protein function. A practical approach, 2nd Edition, Oxford

university press.

3. Edited by T E Creighton, Protein function. A practical approach. Oxford university

press.

4. Cleland and Craik, Protein Engineering, Principles and Practice, Vol 7, Springer

Netherlands.

5. Mueller and Arndt., Protein engineering protocols, 1st Edition, Humana Press. 6. L. Alberghina, Protein Engineering for industrial biotechnology, Harwood Academic

Publisher

B.Tech (Biotechnolgy) B.Tech (Biotechnolgy) B.Tech (Biotechnolgy) B.Tech (Biotechnolgy) detail detail detail detail Syllabus for Admission Syllabus for Admission Syllabus for Admission Syllabus for Admission Batch 2015Batch 2015Batch 2015Batch 2015----16161616 7th Semester7th Semester7th Semester7th Semester

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Seventh Semester


eek

L/T

Credit

Theory

University

Marks

Internal

Evaluation

Hours/

Week

L/T

Credit

Practical

Marks

GS Nano Science & Bio

Technology

3-1 4 100 50

PE Environmental

Biotechnology

3-1 4 100 50

PE Medical &

Pharmaceutical

Biotechnology

3-1 4 100 50

OE Soft Computing */

Other subjects

3-1 4 100 50

PC Advance Lab-II/ Project 8 4 200

Projects on Internet of

Things

8 4 200

Total 16 16 400 200 16 8 400

Total Marks: 1000

Total Credits: 24

Honours Ethics and IPR in

Biotechnology

4 4 100 50

Minor

Specializati

on


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7TH SEM

NANO SCIENCE & BIO TECHNOLOGY

"will be uploaded soon"


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DOWNSTREAM PROCESS ENGINEERING Module -I: Introduction; An overview of bioseparation. Role and importance of Bioseparation process in

biotechnological processes. Problems and requirements of bioproduct purification. Cost- cutting

strategies Characteristics of biological mixtures – Process of Classification of Bioproducts -

Biological activity Analysis of purity-Process economics-Capital and operating cost analysis,

Separation of cells and other insolubles from fermented broth. Filtration and microfiltration,

centrifugation (batch, continuous, basket).

Module -II: Cell disruption: Physical methods (osmotic shock, grinding with abrasives, solid shear, liquid

shear), Chemical methods (alkali, detergents), Enzymatic methods Ultra-filtration: Semipermeable

membranes, membrane geometry and ultrafiltration module configuration. Chromatography in

bioseparation.

Module-III: Separation of soluble bio-products: Liquid-liquid extraction, aqueous two-phase extraction,

precipitation, adsorption. Other bioseparation techniques like Dialysis, electro-dialysis, Liquid

Electrophoresis. Products polishing : Crystallization and drying.

Text Books: 1. M.R. Ladisch, Bioseparations Engineering, Wiley Interscience 2001

2. Kennedy and Cabral, Recovery processes for biological materials.

3. Heinemann, Product Recovery in Bioprocess Technology, Butterworth Publication.

4. Roger G. Harrison, Paul W. Todd, Scott R. Rudge, and Demetri Petrides, Bioseparations

Science and Engineering, Oxford University Press, USA (October 31, 2002)

5. Belter PA and Cussler E, “ Bioseparations ”, Wiley 1985

Reference Books

1. Wankat P.C, “ Rate controlled separations ”, Elsevier, 1990

2. Asenjo J.M., “ Separation processes in Biotechnology ” Marcel Dekker Inc. 1993.


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ENVIRONMENTAL BIOTECHNOLOGY Module-I

Introduction to environmental biotechnology, Environmental monitoring bioreporter, biomarker.

Bioprospecting, Biomicroelectronics and biosensor technology

Introduction to environmental pollutants: Water, Soil and Air: their sources and effects. Removal of

Specific Pollutants: Sources of Heavy Metal Pollution, Microbial Systems for Heavy Metal

Accumulation, Biosorption & detoxification mechanisms. Microbiology and biochemistry of waste

water treatment: Biological Treatment of anaerobic and aerobic; methanogenesis, methanogenic,

acetogenic, and fermentative bacteria- technical process and conditions; Use of Genetically

Engineered Organisms. emerging biotechnological processes in waste - water treatment;

Applications include treatment of municipal and industrial wastewaters,

Module-II

Biodegradation of xenobiotic compounds: Xenobiotic compounds : Aliphatic, Aromatics,

Polyaromatic Hydrocarbons, Polycyclic aromatic compounds, Pesticides, Surfactants and microbial

treatment of oil pollution. Biotransformations and biocatalysts: Basic organic reaction mechanism,

Common prejudices against Enzymes. Advantages & Disadvantages of Biocatalysts, Isolated

Enzymes versus whole cell systems. Mechanistic Aspects and Enzyme Sources. Biocatalytic

Application, Catalytic Antibodies; Stoichiometry, kinetics, and thermodynamics of microbial

processes for the transformation of environmental contaminants.

Module-III

Biooxidation & microbial leaching: Biooxidation – Direct and Indirect Mechanisms, Recovery of

metals from solutions; Microbes in petroleum extraction; Microbial desulfurization of coal.

Clean technologies: Composting Technology and Organic farming, biofertilizers, biopesticides,

microbial polymer production and bio plastic technology. Biotechnology of fossil fuels:

desulfurization of coal, oil shales, microbial enhanced oil recovery (MEOR). Biofuels: Biogas

technology, biohydrogen, bioethahnol production. Biotechnology of mineral processing. Ethical

issues in environmental biotechnology and regulatory framework.

Text Books 1. Rittmann B and McCarty P, Environmental Biotechnology Principles and Applications Mc

Graw Hill 2001

2. Evans, G.M., Furlong, J C.,” Environmental Biotechnology- Theory and application”, John

Wiley & Sons, Ltd, USA. 2003

3. Environmental biotechnology, Scragg Alan, Oxford University Press, 2005

4. Environmental Microbiology, W.D. Grant & P.E. Long, Blakie, Glassgow and London.

5. Microbial Gene Technology, H. Polasa (ED.) South Asian Publishers, New Delhi.

6. Biotreatment Systems, Vol. 22, D. L. Wise (Ed.), CRC Press, INC.

7. Standard Methods for the Examination of Water and Waste Water (14 th Education) , 1985.

American Public health Association

8. Environmental Biotechnology by Bruce Rittmann and Perry McCarty 6. Biotransformations :

K. Faber (1995), Springer- Verlag.


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MEDICAL AND PHARMACEUTICAL BIOTECHNOLOGY Module-I Drug Development in Pharmaceutical Process Production of pharmaceuticals by genetically engineered cells (hormones, interferon),

Microbial transformation for production of important pharmaceuticals (steroids and semi-synthetic

antibiotics), Techniques for development of new generation antibiotics, Protein engineering, drug

design, drug targeting

Module-II Disease Diagnosis and Therapy ELISA and hybridoma technology, Use of enzymes in clinical diagnosis, Use of biosensors for rapid

clinical analysis, Diagnostic kit development for microanalysis, Genetic diseases and DNA based

diagnoses, DNA vaccine, Gene Therapy, Toxicogenomics

Module III Proteomics in Drug Development Role of Proteomics in Drug Development, Diagnosis of disease by Proteomics, Separation and

identification techniques for protein analysis, Development of antibody based protein array for

diagnosis

Text Books

1. Balasubramanian, Bryce, Dharmalingam, Green and Jayaraman(ed), Concepts in

Biotechnology, University Press, 1996

2. Epenetos A.A.(ed), Monoclonal antibodies: applications in clinical oncology, Chapman and

Hall Medical, London

3. Text book of industrial pharmacy by S R Hiremath, Orient Black Swan publication

DOWNSTREAM PROCESS ENGINEERING LAB 1. Harvesting cells using filtration or centrifugation techniques, identification of extracellular

products (enzyme / poly saccharide / some other traceable element) from fermentation

broth or shake-flask culture broth.

2. Compare the centrifugation of bacterial culture and ethanol precipitated DNA and calculate

relative centrifugal force for the two centrifugations

3. Chemical cell disruption and assay for intracellular products

4. Mechanical cell disruption and assay for intracellular products

5. Protein fractionation using precipitation/solvent extraction techniques.

6. Protein fractionation using chromatography (gel filtration and ion-exchange)

7. Separation of proteins and DNA using affinity chromatography

8. Gas chromatography/HPLC


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HONORS: ETHICS AND IPR IN BIOTECHNOLOGY

Module-I: Concept of property, rights, duties and their correlation; Intellectual property rights and its types-

Patents, Trademarks, Copyright & Related Rights, Industrial Design, Traditional Knowledge,

Geographical Indications, Protection of new GMOs; Process patent vs product patent; International

framework for the protection of IP; IP as a factor in R&D; IPs of relevance to Biotechnology and few

Case Studies; Introduction to History of GATT, WTO,WIPO and TRIPS.

Module-II: Basic requirement of a patentable invention- novelty, inventive step, Prior art and Stae of art;

Patent databases; Searching International Databases; Analysis and report formation; Indian

Patent Act 1970 and Recent Amendments; Filing of a patent application; Precautions before

patenting-disclosure/non-disclosure; WIPO Treaties; Budapest Treaty; PCT and Implications;

Role of a Country Patent Office; Procedure for filing a patent, International patenting-requirement,

Patent infringement- meaning, scope, litigation, remedies; Case studies and examples-Rice, Neem

etc.

Module-III: Introduction to Biosafety regulations; Primary Containment for Biohazards and Biosafety Levels;

Biosafety Levels of Specific Microorganisms; Recommended Biosafety Levels for Infectious Agents

and Infected Animals; Biosafety guidelines - Government of India. Definition of GMOs & LMOs; Roles

of Institutional Biosafety Committee, RCGM, GEAC etc. for GMO applications in food and agriculture;

Environmental release of GMOs; Overview of

National Regulations and relevant International Agreements including Cartagena Protocol. Concept

of Bioethics, Public concerns on Human genome research and transgenics- Genetic testing and

screening, Ethics in clinical trials and GCP, ELSI & Human genome projects; Ethics in human

cloning-a case study.

Text Book 1. Stanley SA, Bioethics, Wisdom educational services

2. Sateesh MK, Bioethics and Biosafety, IK International Pvt. Ltd.

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