INTEGRAL UNIVERSITY LUCKNOW

SYLLABUS

of

B.TECH.

BIOTECHNOLOGY 2016-17

SYLLABUS & EVALUATION SCHEME

FOR

B. TECH. BIOTECHNOLOGY

STUDY & EVALUATION SCHEME

B. TECH. Biotechnology (w.e.f. – January 2017)

2nd Year IV Semester

Sl. No.

Course Category

Subject Code

Name of the Subject

Periods Evaluation Scheme

Subject Total

Sessional (CA) End Sem. Exam. (ESE) L T P C CT TA Total

1. DC BE-207 Immunology 3 1 0 4 25 15 40 60 100

2. DC BE-208 Enzymology 3 1 0 4 25 15 40 60 100

3. DC BE-209 Molecular Biology 3 1 0 4 25 15 40 60 100

4. DC BE-210 Introduction to Bioinformatics 2 1 0 ** 25* 15* 40* 60* 100*

5. ESA ME-222 Heat Transfer Operations 2 1 0 3 25 15 40 60 100

6. DE

BE-213 Plant Biochemistry

2 1 0 3 25 15 40 60 100 BE-214 Clinical Biochemistry

BE-215 Applied Thermodynamics for Bioengineers

7. ESA ES -202/ CS-203

Disaster Management/ Cyber Law & Information Security 2 1 0 3 25 15 40 60 100

8. DC BE-211 Immunology Lab 0 0 4 2 30 30 60 40 100

9. DC BE-212 Molecular Biology Lab 0 0 4 2 30 30 60 40 100

10. ESA ME-224 Heat Transfer Operation Lab 0 0 4 2 30 30 60 40 100

Total 17 7 12 27 240 180 420 480 900 ** A non-credit foundation course. Candidate has to pass the course by securing at least 50 % marks up to fourth

semester

L – Lecture T – Tutorial P – Practical C – Credits CT – Class Test TA – Teacher

Assessment

Sessional Total (CA) = Class Test + Teacher Assessment

BS – Basic Sciences DC – Departmental Core HM – Humanities OE – Open Elective DE – Departmental Elective ESA– Engineering Sciences & Arts (Foundation Course & Engineering Courses)

IMMUNOLOGY BE-207

(w.e.f. – January 2017) L T P C

3 1 0 4 Unit I [8] THE IMMUNE SYSTEM: Introduction, Characteristics of innate and adaptive immunity, Humoral and cell mediated immunity, Cells and molecules of immune system, Primary and Secondary lymphoid organs, antigens, antigenicity and immunogenicity, Factor affecting the immunogenicity. Unit II [8] IHUMORAL IMMUNITY: B-Iymphocytes and their activation, structure and function of immunoglobulin, immunoglobulin classes and subclasses, Clonal selection of antibody production, polyclonal and monoclonal antibody and diagnosis, idiotypic antibodies, Structure and function of MHC molecules. Unit III [8] CELLULLAR IMMUNOLOGY: Thymus derived Lymphocytes (T Cells) their classification, antigen presenting cells (APC), Exogenous and endogenous pathways of antigen processing and presentation, cytokines and their role in immune response macrophage activation and granuloma formation, immunosuppression, immune tolerence. Structure, function and application of cytokines. Unit IV [8] ANTIGEN AND ANTIBODY REACTION: serological techniques-ELISA, RIA and Immunoblotting. IMMUNITY TO INFECTION: Hypersensitivity reactions, complement system adjuvants, dose of antigens, types of vaccine preparation, Immunity against infectious diseases (virus, bacteria and protozoan). Unit V [8] TRANSPLANTATION: Graft rejection, evidence and mechanisms of graft rejection, prevention of graft rejection, immunosuppressive drugs, HLA and disease, mechanisms of immunity to tumour antigens. AUTO IMMUNITY: Auto antibodies in humans, pathogenic mechanisms, experimental models of autoimmune disease treatment of auto immune disorders and cancer. Books recommended: 1. Essential Immunology- I. M. Roitt, 10th ed., Blackwell Scientific Publications, Oxford- 4th ed. 2. Kuby’s Immunology- Murry, 5th ed. 3. Ivan M. Roitt, J. Brostoff and David K. Male. Immunology, Glower Medical Publishers, London. 4. Basic and Clinical Immunology, 5th ed., D. P. Sites, J. D. Stobo, H. H. Fudenberg and J. V. Wells. Longe Medical Publications, Singapore.

ENZYMOLOGY BE-208

(w.e.f. – January 2017) L T P C

3 1 0 4 Unit I [8] Introduction, classification and nomenclature of enzymes. Active site, isoenzymes, coenzymes, cofactors, turn over number, enzyme specificity, enzyme activity, specific activity, multienzyme complexes, Physico-chemical characteristic of enzymes. Effect of pH, temperature and substrate concentration on enzyme actvity. UnitII [8]Enzyme kinetics, derivation of Michelis Menten equation, measurement of Km and Vmax, Enzyme inhibition, kinetics of competitive, non-competitive and un-competitive inhibition of enzymes. Effect of pH, temperature and substrate concentration on enzyme kinetics. Allosteric enzymes and their kinetics. Unit III [8] Introduction to industrial enzymes: Topoisomerases, chymotrypsin, glyceraldehyde 3- phosphate dehydrogenase, lysozyme, carboxypeptidase, ribonuclease, lactases and ribozyme and their mechanism of action. Unit IV [8] Molecular folding and defolding of enzymes. Stability of enzymes. Methods of enzyme immobilizations and their applications. Unit V [8] Isolation, purification, and characterization of the enzymes from microorganisms. Intra cellular and extra cellular enzymes, Methods for lysis of plant, animal and microbial cell. Use of detergents in isolation of membrane proteins. Industrial, diagnosis and therapeutic application of enzymes. Books recommended: 1. Methods in enzymology series 2. Murray Moo-Young- Comprhensive Biotechnology, II & IV th Vol. 3. Biochemistry- D. J. Voet & J. G. Voet, 6th ed. 4. Methods in Enzymology- A series. 5. Enzyme Kinetics- Hans Bisswanger, Wiley Publication 6. Biocatelysis: Fundamentals and Application- A.S. Bommarius et al., Wiley Publication

MOLECULAR BIOLOGY BE-209

(w.e.f. – January 2017)

L T P C 3 1 0 4

Unit I [8] Central Dogma, Mutation: Spontaneous, induced; Chemical and physical mutagens; Non sense mutation; Missense mutation; Frame shift mutation; Suppressor mutation; Different methods of DNA repair and SOS response; transposable elements: retrotransposon, mechanism of transposition. Unit II [8] DNA replication in prokaryotes: Roles of DnaA, Helicase, HD protein, Primase, DNA gyrase, Topoisomerase, DNA Polymerase III, DNA Polymerase I, DNA ligase, Fidelity, Regulation; DNA replication in eukaryotes: Autonomously replicating sequence, Origin recognition complex, Minichromosome maintenance proteins, DNA polymerases α, δ, ε, Nucleases, DNA ligase, Telomeres, Regulation; Mitochondrial DNA replication; Reverse transcriptase. Unit III [8] Transcription in prokaryotes: Outline of process, Promoter, RNA polymerase; Transcription in eukaryotes: Outline of process, Promoters, Enhancers, RNA polymerase I, II, III; Post transcriptional modifications: End modifications (Addition of 5` cap and 3‟ Poly A tail in mRNA), RNA splicing - Self splicing and Spliceosome mediated splicing, Cutting events or action of ribonucleases, Covalent modifications, RNA editing, Alternative splicing. Unit IV [8] Genetic code, Wobble hypothesis; Translation in prokaryotes and eukaryotes: Outline of process, Types of RNA, Structure of ribosome, Aminoacyl RNA transferase. Unit V [8] Post - translational processing: Intein splicing, Chemical modification, Proteolytic cleavage, Zymogen activation; Regulation of gene expression: Operon concept: Lac and Trp. Books recommended: 1. Albert B, Bray Denis et al.: Molecular Biology of The Cell, latest ed. 2. Watson, Hopkin, Roberts et al.: Molecular Biology of the Gene, 4 th ed 3. Genetics – Strickberger, 2 nd . 4. Microbial Genetics- D. Frifielder 5. Baltimore- Molecular Biology of the Cell 6. Benjamin Levin - Genes VIII, 8 th ed. 7. Advance Genetics by G.S. Miglani, Narosa Publishing House

INTRODUCTION TO BIOINFORMATICS BE-210

(w.e.f. January 2017) L T P C 2 1 0 0

Unit I [08]

Definition of Bioinformatics, Biological databases: Nucleotide databases (e.g., GenBank,

EMBL, and DDBJ), Biological search engines (e.g., Entrez, SRS, and ARSA).

Unit II [08]

Biological databases: Protein databases- Primary protein databases (e.g., SwissProt, Tr-EMBL,

and PIR), Secondary protein databases (e.g., PROSITE, PRINTS, IDENTIFY, BLOCK, and

PFAM), Composite database (e.g., OWL, and NRDB).

Unit III [08]

Biological databases: Literature databases (e.g., PubMed and PubChem), Biological file formats

(e.g., GenPept/GenBank, FASTA, and EMBL), Applications of Bioinformatics.

Practicals [08]

1. Retrieval of nucleotide sequences from different nucleotide databases and their analysis.

2. Retrieval of protein sequences from different protein databases and their analysis.

3. Retrieval of small chemical compounds from PubChem compound databases and their

analysis.

4. Briefing about retrieval of scientific articles from PubMed database.

Books recommended:

1. D. W. Mount: Bioinformatics-sequence and genome analysis, Cold Spring Harbor lab Press.

2. Jin Xiong: Essential Bioinformatics, Cambridge University press.

HEAT TRANSFER OPERATIONS ME-222

(w.e.f.- January 2017) L T P C

2 1 0 3 Unit I [8] Introduction to heat transfer and general concepts of heat transfer by conduction, convection and radiation. Conduction: Basic concepts of conduction in solids, liquids and gases, steady state temperature fields and one dimensional conduction without heat generation e.g. through plane walls, Cylindrical, Spherical surface and Composite layers etc. Insulation materials, critical and optimum insulation thickness. Extended surface, fines and their practical applications. Introduction to unsteady state heat transfer. Unit II [8] Convection: Fundamentals of convection, Basic concepts and definitions, Natural and forced convection, Introduction to hydrodynamics and thermal boundary layers, Dimensional analysis of force and free convection, Heat transfer with phase change: Condensation of pure and mixed vapors, film wise and drop wise condensation, heat transfer in boiling liquids Unit III [8] Heat Transfer equipment: Classification, principles and design criteria, types of exchanger’s viz. double pipe, shell and tube, plate type, Fouling factors; Overall heat transfer coefficient; Logarithmic mean temperature difference (LMTD) method. Furnaces and their classification and applications Unit IV [8] Radiation & Evaporation: Basic laws of heat transfer by radiation, black body and gray body concepts, view factors, Krichoff‟s law, Black-body radiation exchange, solar radiations, Combined Heat Transfer Coefficient by convection and radiation, Elementary principles, types of evaporators, Single and multiple effect evaporators Books Recommended: 1. Holman, J. P. "Heat Transfer" McGraw Hill Books Co. 2. Mc Adams W.H. "Heat Transmision" McGraw Hill Books Co. 3. Kern D.Q. " Process Heat Transfer" McGraw Hill Books Co. 4. Bedger W.L. and Bancharo J. T. "Introduction to Chemical Engineering" Tata McGraw publication House. 5. Heat Transfer; R. Yadav, Central Publishing House, Allahabad.

DISASTER MANAGEMENT ES-202

(w.e.f.- Session 2016-2017)

L T P C 2 1 0 3

Objective: The objective of this course is to familiarize the student with basic management principles relating to disaster management and mitigation techniques. Units I [8] Concept of disaster management. Types of disaster and their impact: Natural and Man- Made, Like- Earthquakes, Floods, Tsunami, Droughts, Cyclones, Avalanches, Forest Fire, Terrorism related Disaster etc. Assessment of human and Economic losses. Units II [8] Impact of extensive Industrialization. Impact of Global Warming and Environmental Degradation. National and global disasters. Units III [8] National policy for disaster Management, Elementary Knowledge of the Disaster Management Act 2005. Types of Responses: Central, State, District Level, Peoples Community participation in Disaster Management. Post-Disaster Management and Rehabilitation measures. Unit IV [8] Capacity Building for meeting disasters. Long term measures for preventions of disasters. Mitigation technique/strategies: Early warning systems, Data sharing at national and international level. References: 1. Disaster management by Dr. V.K Sethi 2. The great Sumatra Earthquakes and Indian Ocean tsunami of December 2004- the effects of main land India and in the Andaman and Nicobar Island, published by IIT Kanpur. 3. Environmental managaenet by Dr. Shakeel Ahmad 4. Hazards, disasters and your community, ministry of home affairs.

CYBER LAW AND INFORMATION SECURITY CS-203

(w.e.f.- session 2016-2017)

L T P C 2 1 0 3

Unit1 [8] Fundamentals of Cyber Law: Jurisprudence of Cyber Law, Object and Scope of the IT Act 2000, Introduction to Indian Cyber Law, Unicitral Model Law, ISP Guideline. Intellectual property issues and cyber space, Indian perspective, Overview of Intellectual property related legislation in India, Patent, Copy Right, Trademark law, Law related to semiconductor layout & design. Unit 2 [8] E - Commerce: Security Threats to E - Commerce, Virtual Organization, Business Transactions on Web, E-Governance and EDI, Concepts in Electronics payment systems, E-Cash, Credit/Debit Cards, E- Agreement, Legal recognition of electronic and digital records, E-Commerce Issues of privacy, Wireless Computing- Security challenges in Mobile devices. Digital Signatures - Technical issues, legal issues, Electronic Records, Digital Contracts, Requirements of Digital Signature System. Unit 3 [8] Investigation and Ethics: Cyber Crime, Cyber jurisdiction, Cyber crime and evidence act, Treatment of different countries of cyber crime, Ethical issues in data and software privacy, Plagiarism, Pornography, Tampering computer documents, Data privacy and protection, Domain Name System, Software piracy, Issues in ethical hacking. Internet security treats: Hacking, Cracking, Sneaking, Viruses, Trojan horse, Malicious Code & logic bombs. Introduction to biometric security and its challenges, Finger prints. Cyber crime forensic: CASE STUDY in Cyber Crime. Unit 4 [8] Information security- Information Systems and its Importance, Role of Security in Internet and Web Services, Principles of Information Security, Classification of Threats and attacks, Security Challenges, Security Implication for organizations, Security services - Authentication, Confidentiality, Integrity, Availability and other terms in Information Security, Information Classification and their Roles. Introduction to Cryptography, Issues in Documents Security, Keys: Public Key, Private Key, Firewalls, Basic Concepts of Network Security, Perimeters of Network protection & Network attack, Need of Intrusion Monitoring and Detection. References: 1. Harish Chander “Cyber Law and IT Protection” , PHI Publication, New Delhi 2. Merkov, Breithaupt “ Information Security”, Pearson Education 3. “Cyber Law in India” - Farooq Ahmad-Pioneer books. 4. K. K. Singh, Akansha Singh “Information Security and Cyber law”, Umesh Publication, Delhi

IMMUNOLOGY LAB BE-211

(w.e.f.- January 2017)

L T P C 0 0 4 2

1. Isolation, purification and identification of Antibodies. 2. Double diffusion techniques for identification of Antigen-Antibody

samples. 3. Immunoelectrophoresis techniques. 4. ELISA (Enzyme Linked Immunosorbent Assay) 5. Immunoblotting Using ELISA-dot 6. Western blot technique (Demonstration) 7. ABO blood group typing 8. RBC and WBC count by Heamocytometer

MOLECULAR BIOLOGY LAB BE-212

(w.e.f.- January 2017)

L T P C 0 0 4 2

1. Estimation of DNA content in the given sample by diphenylamine method.

2. Estimation of RNA content by the Orcinol method.

3. Determination of Tm of DNA and RNA.

4. Isolation of plasmid DNA.

5. Isolation of bacterial genomic DNA

6. Isolation of plant DNA.

7. Visualization of DNA by Agarose Gel electrophoresis.

HEAT TRANSFER OPERATION LAB ME-224

(w.e.f. – January 2017) L T P C

0 0 4 2

1. To determine the Steffen’s Boltzmann Constant 2. To determine the surface heat transfer coefficient for a vertical tube losing heat by natural Convection. 3. To find the heat transfer coefficient of forced convection in internal pipe flow 4. To determine the efficiency and effectiveness of a fin and study the temperature distribution in a simple pin fin. 5. To study heat transfer through the pipe of different metals. 6. To determine the LMTD, overall heat transfer coefficient and effectiveness of a heat Exchanger working in parallel flow mode. 7. To determine the LMTD, overall heat transfer coefficient and effectiveness of a heat Exchanger working in counter flow mode. 8. To determine the overall heat transfer coefficient for the composite wall and to compare the same with that calculated from the equation.

PLANT BIOCHEMISTRY BE-213

(w.e.f. January 2017) LTPC 2 1 0 3

Unit 1 [8]

Photosynthesis – Photosynthetic apparatus, pigments of photosynthesis, role of carotenoids, photosystems I and II, their location; Hill reaction, photosynthetic electron transport and generation of NADPH & ATP, cyclic and non-cyclic photophosphorylations, complexes associated with thylakoid membranes; light harvesting complexes, path of carbon in photosynthesis – C3 and C4 pathway of carbon reduction and its regulation, Photorespiration.

Unit 2 [8]

Nitrate assimilation, structural features of nitrate reductase and nitrite reductase, incorporation of ammonia into organic compounds, regulation of nitrate assimilation.

Unit 3 [8] Plant Hormones – Biosynthesis of growth regulating substances- auxins, cytokinins, gibberllic and abscisic acids.

Unit 4 [8] Stress metabolism in plants – Environmental stresses, salinity, water stress, heat, chilling, anaerobiosis, pathogenesis, heavy metals, radiations and their impact on plant growth and metabolism, criteria of stress tolerance.

Reference Books

1. Plant Biochemistry by P. M. Dey and J. B. Harborne, Harcourt Aria PTE Ltd. Singapore. 2. Plant Physiology by Salinbury. 3. Plant Physiology by Davin. 4. Lehninger Biochemistry

CLINICAL BIOCHEMISTRY BE-214

(w.e.f. January 2017) LTPC 2 1 0 3

Unit I Hydrogen ion and electrolyte homeostasis [8] Hydrogen ion homeostasis: Factors regulating blood pH- buffers, respiratory and renal regulation. Acid-base balance- causes, biochemical findings and management of metabolic and respiratory acidosis and alkalosis. Water, sodium and potassium homeostasis: Distribution of water and electrolytes in the ECF and ICF. Unit II Inborn errors of metabolism [8] Disorders of amino acid metabolism- aminoaciduria, phenylketonuria, alkaptonuria, cystinuria, and maple syrup urine disease. Disorders of carbohydrate metabolism- glycogen storage diseases, galactosemia, fructose intolerance Disorders of purine, pyrimidine and porphyrin metabolism- Hyperuricemia and gout, Hypouricemia. Disorders of lipid metabolism- lipid storage diseases, fatty liver. Unit III Kidney and nutritional disorders [8] Kidney function tests: Glomerular and tubular function tests. Abnormal constituents of urine. Pathogenesis, biochemical findings and management of glomerulonephritis, renal failure, nephrotic syndrome Protein energy malnutrition- Marasmus and Kwashiorkor. Unit IV Liver and gastrointestinal disorders, Diabetes, and Cancer [8] Liver function tests (excretory, synthetic, detoxification and metabolic). Plasma enzymes in liver disease. Jaundice- neonatal. Gastric function tests. Pathogenesis, biochemical findings and management of peptic ulcer and gastritis. Pancreatic and intestinal function tests. Classification of diabetes. Metabolic abnormalities – glycosuria. Acute and long term complications, Diagnosis and management. Cancer: Differences between benign and malignant tumours. Morphological and biochemical changes in tumour cells. Tumour markers (AFP, CEA, hCG only). Books for Reference: 1. Principles of Internal Medicine. Harrison’s Vol 1 & 2, 14th edition McGraw Hill, 1998. 2. Harper’s Biochemistry McGraw Hill, 27th ed, 2006. 3. Biochemistry – A case oriented approach. Montgomery et al. Mosby. 1990 5th editon. 4. Clinical Chemistry – Principles, procedures, correlations – Bishop, Lippincott, 2005, 5th edition. 5. Guyton and Hall. Textbook of Medical Physiology 12 ed 2011

APPLIED THERMODYNAMICS FOR BIOENGINEERS BE-215

(w.e.f- January 2017) L T P C 2 1 0 3

UNIT I 08 First Law of thermodynamics, a generalized balance equation and conserved quantities, Volumetric properties of fluids exhibiting non ideal behavior; residual properties; estimation of thermodynamic properties using equations of state; calculations involving actual property exchanges. UNIT II 08 Partial molar properties; concepts of chemical potential and fugacity; ideal and non-ideal solutions; concepts and applications of excess properties of mixtures; activity coefficient; composition models. UNIT III 08 Equilibrium criteria for homogeneous chemical reactions; evaluation of equilibrium constant; effect of temperature and pressure on equilibrium constant; calculation of equilibrium conversion and yields for single and multiple reactions. UNIT IV 08 Thermodynamics of microbial growth stoichiometry thermodynamics of maintenance, Calculation of the Operational Stoichiometry of a growth process at Different growth rates, Including Heat using the Herbert –Pirt Relation for Electron Donor, thermodynamics and stoichiometry of Product Formation. TEXT BOOKS: 1. Smith J.M., Van Ness H.C., and Abbot M.M. “Introduction to Chemical Engineering Thermodynamics”, 6th Edition. Tata McGraw-Hill, 2003. 2. Narayanan K.V. “A Text Book of Chemical Engineering Thermodynamics”, PHI,2003. 3. Christiana D. Smolke, “ The Metabolic Pathway Engineering Handbook Fundamentals”, CRC Press Taylor & Francis Group, 2010. REFERENCE: 1. Sandler S.I. “Chemical and Engineering Thermodynamics”, John Wiley,1989.