C196-E079

Ultra High Performance Liquid Chromatograph

Nexera X2

Maximizing the Potential of

UHPLC/HPLC Analyses

Resolution

Intelligence

Stability

SensitivityIntelligent data solutions, including a new peak calculation method for enhanced separation power, accelerate workflows.

The ultimate platform for high-sensitivity UHPLC and HPLC detection.

Enhanced peak resolution achieved by minimizing external column dispersion.

Superior stability for UHPLC/HPLC analyses.

Ultra High Performance Liquid Chromatograph

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Utilizing patented technologies, and driven by customer input, Shimadzu has developed

the Nexera X2 series to deliver superior, real-world performance across a wider

application range, while delivering unparalleled flexibility and reliability in UHPLC/HPLC

analyses. With no limits and no compromises, the Nexera X2 achieves a new milestone in

the evolution of liquid chromatography.

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Nexera Quaternary system Nexera Method Scouting system

Nexera MP system Nexera TQ system and Nexera SQ system

Nexera SR system Nexera UHPLC/HPLC system

Nexera X2 SeriesA full range of configurations to meet your applications needs…

features superior sensitivity, resolution and stability. * offers new separation technology.

is a flexible quaternary gradient system that is HPLC and UHPLC compatible.

offers comprehensive method development using 96 combinations of mobile phases and columns.

offers ultrafast LC/MS and LC/MS/MS analysis.

is an LC/MS frontend HPLC for high-throughput analysis of multiple samples.

enables switching from UHPLC to HPLC in a single system using a selection of mixers and columns.

Nexera SR system

Nexera Quaternaryyy system

Nexerarara MP system

Nexera UHPLC/HPLC systemN

*Intelligent Peak Deconvolution Analysis

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The CBM-20A enables system control via a network. The CBM-20A allows for various

system configuration to meet different laboratory workflows.

Nexera X2 Component

Ultra-high-sensitivity and high-resolution detector

Th

sys

The SPD-M30A realizes ultra-high sensitivity and minimized

dispersion, supporting the new separation calculation

method, . The temperature-controlled optics provide

stable baseline for genuine UHPLC analysis.

*Intelligent Peak Deconvolution Analysis

SIL-30ACMP

SPD-M30A

Upgradable to binary gradient and quaternary gradient, the

LC-30AD also supports solvent blending for automated

mobile phase preparation.

LC-30AD

SIL-30AC SIL-30AC / Rack Changer II

CTO-30ASCTO-30A CTO-20AC

U

CBM-20A

D e t e c t o r

A u t o s a m p l e r

C o l u m n o v e n

S o l v e n t d e l i v e r y u n i t

Multifunctional autosampler;

pretreatment and loop injection

are available

Features control to a maximum 150°C

(Max column length: 150 mm)

Large space for placing multiple

columns and valves

(Max column length: 300 mm)

Compact column oven for LC/MS

front end

(Max column length: 50 mm)

Maximum 12 plates can be set with this

combination

Open-access autosampler with

maximum 6 plates

5Ultra High Performance Liquid Chromatograph

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Intelligence in UHPLC/HPLC Analysis

Complete Separation of Co-eluted Peaks by Intel l igent Peak Deconvolution Analysis ( )

Example1: Remove tai l ing peak

Example2: Impurity peak extracted from co-eluted peak

enables the extraction of a single peak from co-eluted peaks by utilizing difference in spectra by a few steps. The new separation

method removes discussion of integration methods for co-eluted peaks. The also helps detect impurity peaks in a target peak.

Vertical separationBaseline separation

Removes peak integration ambiguities

Step 2Extraction of single peak signal using

the difference between each spectrum

Step 1Analysis

Step 3Display of single component

representative peak

Component A

Component B

AU

1

2

nm

Component A

shows extraction of component B.

shows extraction of component A.

::

Component B

0.00 0.25 0.50 0.75 1.00 1.25 min

0.5 mg/L spiked

0.488 mg/L measured

(98% accuracy)

0.00 0.25 0.50 0.75 1.00 1.25 min

Target peak

Tailing peak

The target peak is on the tailing peak

This peak looks a single peak.

Remove the tailing peak!!

By removing the main peak, the impurity peak appears on the chromatogram!!

0.22 0.24 0.26 0.28 min0.22 0.24 0.26 0.28 min

Peak purity curve

1

2

12

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Ultimate Sensitivity

Ultra High Performance Liquid Chromatograph

Achieving maximum sensitivity

Column Shim-pack XR-ODSⅢ (50 mmL. × 2.0 mmI.D., 1.6 μm)

Flow rate 0.6 mL/min

Main peak propyl 4-hydroxybenzoate

0.005％ Impurity peak ethyl 4-hydroxybenzoate

μAU

mAU

1750

600

S/N 9.53

0.5 0.7 0.9 min

400

200

0

1250

750

250

0

0.0 0.5 1.0 min

Mai

n pe

ak

0 50.5 0 70.7 0 90.9 m

Impurity peak

Section of capillary cell

The SR-Cell features high-sensitivity and low-dispersion by adopting total reflection capillary cell.

High-sensitivity and high-resolution

SR-Cell

The SPD-M30A photodiode array detector adopts the high-sensitivity and low-dispersion capillary cell, SR-Cell, which achieves a

0.4×10-5 AU noise level. The SPD-M30A offers ultimate in UHPLC analysis, realizing high-sensitivity and high-resolution.

The excellent sensitivity and wide dynamic detection range enables simultaneous analysis of a main ingredient and impurity of

0.005% content.

The SR-Cell is available for UHPLC and HPLC, realizing high-sensitivity analysis across a wide analytical range.

0.0 1.0 2.0 3.0 4.0 min

0.0 5.0 10.0 15.0 20.0 25.0 min

Conditions

Conditions

Column

Time program

Flow rate

Shim-pack VP-ODS (150 mmL. × 4.6 mmI.D., 4.6 μm)

2% (0 min)→100% (30 min)

1.0 mL/min

Conditions

Column

Time program

Flow rate

Shim-pack XR-ODSⅡ (75 mmL. × 3.0 mmI.D., 2.2 μm)

2% (0 min)→100% (4.2 min)

1.3 mL/min

SR Cell

The SR-Cell features high-sensitivity by adopting total reflection capillaryThe SR-Cell features high-sensitiv

Section of capillary cellSection of capillary cell

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Maximized Resolution

Superior peak shape for maximum peak resolution

In ultrafast analysis, external column dispersion affects peak resolution. The SR-Cell in the SPD-M30A has minimized the cell volume to

realize excellent peak shape with higher peak resolution, supporting genuine UHPLC analysis.

Superior spectrum resolution and l inearity

The SPD-M30A provides unrivaled spectrum resolution by adopting an optimized optic system. In addition, the new signal treatment

technology realizes excellent spectrum linearity across a wide area from low concentration to high concentration. The SPD-M30A supports

analyses, such as purity analysis, that require a wide dynamic range.

Nexera SR system for high-sensitivity and high-resolution

Nexera SR system is configured with the new SPD-M30A photodiode array detector.

The new peak calculation technology, , proposes a new approach for separation

of co-eluted peaks and extraction of impurity peaks from main peaks. The superior

sensitivity and resolution expand the range of UHPLC analyses.

*As of November 2012, according to Shimadzu research.

A Benzene spectrum is often used for evaluation of spectrum resolution.

The SPD-M30A shows world’s highest spectrum resolution, B/A=2.90*.

The SPD-M30A shows excellent spectrum linearity in the analysis of

caffeine samples with different concentrations.

Column Shim-pack XR-ODS III(50 mmL. × 2.0 mmI.D., 1.6 μm)

Flow rate 0.6 mL/min

Peak No.

Tailing factor (Tf5%)

Nexera X2 Vendor A

1

2

3

1.309

1.297

1.273

1.546

1.486

1.432

0.20 0.25 0.30 0.35 0.40 0.45 0.50 min

peak

1

peak

2

peak

3

Nexera X2

Vendor A UHPLC

230 240 250 260 270 280 nm

0

B

A

B/A=2.90

Caffeine 100 mg/LCaffeine 1000 mg/L

250 300 350 nm(Normalize)

ｍAU

Similarity 0.9998

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Nexera SR system

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Unrivaled Stability

Ultra High Performance Liquid Chromatograph

Baseline stabil ization by temperature-controlled TC-Optics

The SPD-M30A adopts the new temperature-controlled TC-Optics with the SR-Cell that has an optimized heat exchange inlet pipe.

These features achieve faster stabilization times low external dispersion for UHPLC analysis.

The faster stabilization shortens the “wait time” for analysis, thereby increasing total analytical throughput.

Stable performance against room temperature shift

The SPD-M30A incorporates TC-Optics to achieve a more stable baseline against room temperature shifts.

Flow rate 0.5 mL/min

Mobile phase Methanol

Detector PDA 254 nm

Temperature RT

Vendor A PDA detector

SPD-M30A

0 10 20 30 40 50 min

-40

-30

-20

-10

10

0

mAU

The SPD-M30A shows a stable baseline faster after

startup while the detector from vendor A shows

baseline drift during 1 hour.

le the detector from vendor A shows

ft during 1 hour.

SPD-M30A

Flow rate 0.5 mL/min

Mobile phase Methanol

Detector UV 254 nm

0 100 200 300 400 500 600 min-20

-15

-10

-5

0

5

10

15

20

25

mAU

254 nm

Room temperature

Vendor A PDA detector

SPD-M30A25°C

30°C

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Ultimate Flexibility

The modular nature of Nexera X2 allows a

solvent blending function that can mix

solvents according to desired ratios. This

function enables dilution of solvent, buffer

preparation and modifier addition for mobile

phase preparation.

Buffer pH can be optimized using the solvent

blending function as shown to the right

chromatograms. The function is a useful tool

for optimizing mobile phase conditions in

method development.

Measured pH of mobile phase prepared by solvent blending

Automation of mobile phase preparation by solvent blending function

The solvent blending function can be added to a Nexera UHPLC/HPLC switching system

by upgrading the configuration. The system supports all processes from method

development to method transfer from HPLC to UHPLC.

Combination of UHPLC / HPLC Switching system and solvent blending

Pump1

Pump2

A

B

C

D

A

B

C

D

Y

X

85%

10%

50%

20%

5%

25%

5%

0%

Solvent BlendingAutomated mobile phase preparation

Binary GradientGradient elution by prepared solvents

0.0 1.0 2.0 3.0 4.0 min

Blending 1

1, 2, 3

pH 3.0A / B = 6 / 4

0.0 1.0 2.0 3.0 4.0 min

Blending 2

1

23

pH 7.2A / B = 2 / 8

1, Benzoic ac id2, Sorbic ac id3, methyl 4-hydroxybenzoate

Blending 1 3.010

Blending 2 7.192

3.004

Solvent blending Manual preparation

7.191Blending 1 A / B = 6 / 4

Blending 2 A / B = 2 / 8

Pump Y solvent Methanol

A; 20 mmol/L phosphoric acid aqueous solutionB; 20 mmol/L disodium hydrogen-phosphate

Pump X solvent

em

lvent blending

Nexera UHPLC/HPLC system

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Ultra High Performance Liquid Chromatograph

Nexera X2 autosamplers support ultrafast injections to increase analytical throughput. In particular, the SIL-30ACMP achieves a 7-second injection

time and a 14-second cycle time, making it ideally suited for ultrafast analysis.

Ultrafast 7-second injection and 14-second cycle t ime

The Nexera X2 autosamplers, SIL-30AC and SIL-30ACMP, provide near-zero carryover. The improved design maintains a low carryover level, even

during long-term usage, to ensure reliable analysis.

Both the SIL-30AC and SIL-30ACMP support the use of multiple rinse

solvents for rinsing the needle’s outer surface and inner surface to

thoroughly minimize carryover.

The carryover level was extremely low even after 30,000 injections.

Minimizing carryover with multiple r inse solvents

Enhanced UHPLC Reliability

0

25000

50000

75000

100000

125000

1:235.40>86.10(+)2:256.10>167.10(+)3:281.10>86.10(+)

0.0 0.25 0.5 0.75 1.0 min

14 sec

Contact surfaces of needle and needle port

where carryover is likely to occur

Port section is rinsed by any of the rinse solutions(1 to 3 solutions)

Discharge to drain port

Needle port(schematic diagram)

Drain

Multi-rinse mechanism canaccommodate up to 4 solutions

Sample loop

Needle

Rinse ports

For rinsing needle surface andinner surface (3 types)

For rinsing needlesurface (1 type)

R0 R1 R2 R3

Shim-pack XR-ODS II (30 mmL. × 1.5 mmI.D., 2.2 μm) Column

Flow rate 1.2 mL/min

Event #

1

2

3

Q1 m/z

235.4

256.1

281.1

Q3 m/z

86.1

167.1

86.1

Compound

Lidocaine

Diphenhydramine

Imipramine

Shim-pack XR-ODS III (150 mmL. × 2.0 mmI.D., 2.2 μm)Column

Mobile phase Water / Methanol = 7 / 3

Flow rate 0.4 mL/min

Sample Caffeine 4000 mg/L

0.0 0.5 1.0 1.5 2.0 2.5 min

-25

0

25

50

75

100

125

150

175

200

ｍAU

2.25 2.50 2.75 min

0

250

500

750uV

0.00018%

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For Research Use Only. Not for use in diagnostic procedures. The contents of this publication are provided to you “as is” without warranty of any kind, and are subject to change without notice. Shimadzu does not assume any responsibility or liability for any damage, whether direct or indirect, relating to the use of this publication.

© Shimadzu Corporation, 2012www.shimadzu.com/an/Printed in Japan 3655-08220-30ANS

Nexera X

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