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ProteinDenaturation:an examination of the critical enzyme
Catalase
London, November 29 2010 - UK Meteorological Office researchers have shown that the world could warm by 4 °C by 2060, devastating much of the Amazon rainforest and changing living conditions throughput the world
Climate change
and an EXPLOSION in human population fueled by the rapid consumption in fossil fuels (7 billion people On Earth estimated this 2012) are Inexorably linked
Scientists agree that increased
Emissions from carbon
based fossil fuels into the atmosphere will result in devastating ecological shifts throughout the globe
Polar ice caps are melting Sea levels are rising Oceans are becoming more acidic Extreme weather events such as heat waves, hurricanes and, most perniciously, drought are becoming more common place
Photo taken on Feb. 24, 2012 shows cracked land in a pond at Fanglang Village in Malong County of southwest China's Yunnan Province. A brutal drought has wracked the province since late last year, leaving at least 3.15 million people without sufficient supplies of drinking water as of Monday, according to government statistics. (Xinhua/Lin Yiguang)
DROUGHT will become more frequent and common place
Extreme DROUGHT in Kenya has displaced more than two million people, put 12 million more in
need of immediate food
assistance and has killedmore than 30%of the county’s cattle-
completely devastatingtribes whose main source of wealth is measured in heads ofcattle.
These cattle died when biological functions could no longer be sustained because of extreme conditions
.Metabolic enzymes function best in aqueous, bufferedsolutions with a constant body temperature of 37degress Celsius.
Their viability under extreme conditions is important to understandThe process of protein/ enzyme breakdown is called
DENATURATION…
The reactivity of catalase is highly visible
Objective:
Students will examine the effects of extreme conditions on biological moleculesThey will formulate their own experimental designs and submit their reports for peer review.
Process:Students will examine the reactivity of the enzyme
Catalase a critical enzyme involved
in removing toxic hydrogen epoxied – a poisonous metabolic by product.
2(H2O2)----catalase---- 2H2O + O2
Students will have the opportunity toexamine catalase reactivity using potato(a rich source of the enzyme and muchneater to work with than beef liver another rich source) of theThey will pick 3 of the 4 conditions to examine
Temperature extremes (freezing vs. hot) Hypertonic (salt) solution vs. control Dehydration vs., control Acidic or basic conditions
Reactivity of catalase under different extremes
Students will work independently while being ‘linked up’
to a group of 3-4 students
They submit lab reports for peer review where their
methodology
experimental design conclusions
will be assessed in a manner similar to the process that Scientists engage e in.