CBS Edition 61 - CAMAG · CBS Edition 61 The CBS classification system 1. Reviews and books a)...

CBS Edition 61The CBS classification system

1. Reviews and booksa) Books on TLCb) Books containing one or several chapters on TLCc) Books containing frequent TLC information spread over severalchapters of other information

2. Fundamentals, theory and generala) Generalb) Thermodynamics and theoretical relationshipc) Relationship between structure and chrom. behaviourd) Measurement of physico-chemical and related valuese) Optimization of solvent systemsf) Validation of methods

3. General techniques (unless they are restricted to the applicationwithin one or two classification sections)

a) New apparatus/techniques for sample preparationb) Separation materialc) New apparatus for sample application/dosaged) New apparatus/techniques for chromatogram developmente) New apparatus/techniques for pre- or postchromatographicderivatizationf) New apparatus/techniques for quantitative evaluationg) New apparatus/techniques for other TLC steps (distinguishedfrom section 4)

4. Special techniquesa) Automation of sample preparation/applicationb) Automation of complex chromatogram developing techniquesc) Automation, computer application in quantitative chromatogramevaluationd) Combination of TLC with other chromatographic techniquese) Combination of TLC with other (non-chromatographic)techniques...MS, IR...etc.

5. Hydrocarbons and halogen derivativesa) Aliphatic hydrocarbonsb) Cyclic hydrocarbonsc) Halogen derivativesd) Complex hydrocarbon mixtures

6. Alcohols7. Phenols8. Substances containing heterocyclic oxygen

a) Flavonoidsb) Other compounds with heterocyclic oxygen

9. Oxo compounds, ethers and epoxides10. Carbohydrates

a) Mono- and oligosaccharides, structural studiesb) Polysaccharides, mucopolysaccharides, lipopolysaccharides

11. Organic acids and lipidsa) Organic acids and simple estersb) Prostaglandinsc) Lipids and their constituentsd) Lipoproteins and their constituentse) Glycosphingolipids (gangliosides, sulfatides, neutralglycosphingolipids)

12. Organic peroxides13. Steroids

a) Pregnane and androstane derivativesb) Estrogensc) Sterolsd) Bile acids and alcoholse) Ecdysones and other insect steroid hormones

14. Steroid glycosides, saponins and other terpenoid glycosides15. Terpenes and other volatile plant ingredients

a) Terpenesb) Essential oils

16. Nitro and nitroso compounds

17. Amines, amides and related nitrogen compoundsa) Amines and polyaminesb) Catecholamines and their metabolitesc) Amino derivatives and amides (excluding peptides)

18. Amino acids and peptides, chemical structure of proteinsa) Amino acids and their derivativesb) Peptides and peptidic proteinous hormones

19. Proteins20. Enzymes21. Purines, pyrimidines, nucleic acids and their constituents

a) Purines, pyrimidines, nucleosides, nucleotidesb) Nucleic acids, RNA, DNA

22. Alkaloids23. Other substances containing heterocyclic nitrogen

a) Porphyrins and other pyrrolesb) Bile pigmentsc) Indole derivativesd) Pyridine derivativese) other N-heterocyclic compounds

24. Organic sulfur compounds25. Organic phosphorus compounds (other than phospholipids)26. Organometallic and related compounds

a) Organometallic compoundsb) Boranes, silanes and related non-metallic compoundsc) Coordination compounds

27. Vitamins and various growth regulators (non-peptidic)28. Antibiotics, Mycotoxins

a) Antibioticsb) Aflatoxins and other mycotoxins

29. Pesticides and other agrochemicalsa) Chlorinated insecticidesb) Phosphorus insecticidesc) Carbamatesd) Herbicidese) Fungicidesf) Other types of pesticides and various agrochemicals

30. Synthetic and natural dyesa) Synthetic dyesb) Chloroplasts and other natural pigments

31. Plastics and their intermediates32. Pharmaceutical and biomedical applications

a) Synthetic drugsb) Pharmacokinetic studiesc) Drug monitoringd) Toxicological applicationse) Plant extracts, herbal and traditional medicinesf) Clinico-chemical applications and profiling body fluids

33. Inorganic substancesa) Cationsb) Anions

34. Radioactive and other isotopic compounds35. Other technical products and complex mixtures

a) Surfactantsb) Antioxidants and preservativesc) Various specific technical productsd) Complex mixtures and non-identified compounds

36. Thin-layer electrophoresis37. Environmental analysis

a) General papersb) Air pollutionc) Water pollutiond) Soil pollution

38. Chiral separations

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1. Reviews and books

61 001 T.A. BRETTELL, R. SAFERSTEIN*, (*New Jersey State Police, Forensic Sci. Bureau, Box 7068, West Trenton, NewJersey 08625, USA): Forensic Science. Anal. Chem. 59, 162R-174R (1987). Review with many references on TLCapplication in forensic science. Also other techniques. Review , forensic science 1

61 002 U.A.TH. BRINKMAN, (Dep. Anal. Chem., Free Univ., De Boelelaan 1083, NL-1081 HV Amsterdam): The relativemerits of planar and column chromatographic methods in organic trace-level analysis. H. TRAITLER, A.STUDER, R.E. KAISER eds): Instrumental HPTLC, Institute for Chromatography, Bad Dürkheim, FRG (1987), 81-92.Discussion of the potential and limitations of column liquid chromatography, planar chromatography and capillary gaschromatography with the emphasis on separation efficiency, sample pretreatment, detection and derivatizationprocedures, and speed of analysis. Mention of on-line coupling of various chromatographic techniques and some futuretrends. Review , organic trace-level analysis 1, 4d

61 003 A.F. GROSS*, P.S. GIVEN JR, A.K. ATHNASIOS, (*Nabisco Brands, Inc., Coporate Technology, Box 1943, EastHanover, New Jersey 07936-1943, USA): Food. Anal. Chem. 59, 212R-252R (1987). A review with numerousreferences on TLC applications in food analysis. Also other techniques. Review , food analysis. 1, 29

61 004 E. PUNGOR (ed.): Handbook of analyst (Hungarian). Müszaki Könyvkiadó, Budapest (1987), 399 pages. Contains150-page review of TLC methods classified into 17 groups of compounds and a survey of reagents. Quality control , review 1b

61 005 TIAFT (The International Association of Forensic Toxicologists): Thin-layer chromatographic Rf values oftoxicologically relevant substances on standardized systems. Report VII of the DFG commission forclinical-toxicological analysis, special issue of the TIAFT bulletin; VCH Verlagsgesellschaft mbH, D-Weinheim 1987.TLC of abt. 1100 substances (drugs, pesticides etc. including metabolites) on silica with 10 different standardizedsolvent systems (single component up to 3-component systems) in comparison with 4 standards each. Review 1a

61 006 H. TRAITLER, A. STUDER, R.E. KAISER (eds): Instrumental high performance thin-layer chromatography. Institute for Chromatography, Bad Dürkheim FRG (1987). Proceedings of the Fourth International Symposium onHPTLC, Planar Chromatography, Selvino, Italy, 22-25. Sep. 1987. 468 pages with 50 contributions, 20 of themseparately abstracted in this CBS issue.

1

2. Fundamentals, theory and general

61 007 K. DALLENBACH-TOELKE, SZ. NYIREDY, S.Y. MESZAROS, O. STICHER*, (*Pharm. Inst., Eidgenössische Tech.Hochschule Zürich, 8092 Zürich, Switzerland): TLC, HPTLC and OPLC separation of ginsenosides. J. High Resol.Chromatogr. 10, 362-364 (1987). Application of the top triangle of the „prisma“ model for mobile phase optimization ofpolar compounds in normal phase chromatography. TLC and HPTLC of ginsenosides on silica with water - methanol -MEK in various proportions. OPLC of the separated ginsenosides on silica with MEK - methanol - water 70:22:8.Detection by spraying with vanillin - sulfuric acid and densitometry at 550 nm. Pharmaceutical research , comparison of methods , ginsenosides , OPLC 2, 32e, 14

61 008 B. DE SPIEGELEER*, W. VAN DEN BOSSCHE, P.DE MOERLOOSE, (*Dep. Pharm. Chem. & Drug Qual. Cont., StateUniv. Ghent, Harelbekestraat 72, B-9000 Gent, Belgium), D.MASSART, (Pharm. Inst., Free Univ., Brussels,Laarbeekstraat 103, B-1090 Brussel, Belgium): A strategy for two-dimensional high-performance thin-layerchromatography, applied to local anesthetics. Chromatographia 23, 407-411 (1987). Presentation of a strategy forthe selection of the two systems in two-dimensional HPTLC based on the absolute values of the correlation matrixelements, and on the correlation coefficient. Demonstration of the separation of 14 local anesthetics byone-dimensional HPTLC. Quantification by densitometry. Pharmaceutical research , quantitative analysis , densitometry , qualitative identification , correlationcriterion , 2-D TLC , anesthetics

2a, 32a

61 009 O. HADZIJA, S. ISKVIC, M. TONKOVIC, („Ruder Boskovic“ Inst., 41000 Zagreb, Yugoslavia): Mobility of



61 009 O. HADZIJA, S. ISKVIC, M. TONKOVIC, („Ruder Boskovic“ Inst., 41000 Zagreb, Yugoslavia): Mobility ofFe(III)-phenolic acid complexes in thin-layer chromatography. J. Chromatogr. 402, 358-360 (1987). TLC ofphenolic acids on Fe(III)-impregnated silica with distilled, tap and sea water. Detection by spraying with bromocresolgreen indicator reagent and a 1% aqueous solution of Fe(III) nitrate, and by inspection under UV. Discussion of themobility of Fe(III)-phenolic acid complexes. Fe(III)-phenolic acid complexes , mobility 2a, 33a

61 010 Z. MAO (Mao Zongxiu), A. WU (Wu Afu), ZH. MAO (Mao Zhixiang), (Dep. Pharm., Zhejiang Med. Coll., Hangzhou, P.R.China): (Numerical taxonomy and information content calculation by computer in thin-layerchromatography. ). (Chinese). Chinese Anal. Chem. 15, 151-152 (1987) (Fenxi Huaxue). Presentation of a methodfor numerical taxonomy and information content calculation of Rf values by a computer. Comparison of the calculatedresults with those of TLC experiments of 24 cations on silica. Numerical taxonomy , information content calculation 2a, 33a

61 011 W. MARKOWSKI, E. SOCZEWINSKI, G. MATYSIK, (Dept. Inorg. Anal. Chemistry, Med. Acad., Staszica 6, 20-081Lublin, Poland): A microcomputer program for the calculation of Rf values of solutes in stepwise gradientthin-layer chromatography. J. Liquid Chrom. 10, 1261-1276 (1987). Microcomputer calculation of Rf values for anygradient program after introduction of Rf values of sample components obtained for several isocratic runs. Goodagreement of calculated and experimental Rf values.

2d

61 012 B. OSCIK-MENDYK, J.K. ROZYLO, (Inst. Chem., M. Curie-Sklodowska Univ., Lublin, Poland): Analysis ofchromatographic parameters in the systems with ternary mobile phases. J. Liquid Chrom. 10, 1399-1415 (1987).Proposition of a new method for the analysis of chromatographic parameters (optimal choosing of the mobile phasecomposition) obtained for ternary mobile phases. Investigation were carried out for 6 model substances (naphthalene,o-nitrotoluene, o-bromonitrobenzene, o-nitroaniline, o-nitrophenol, fluorenone) with single-, two- and three componentsolvent systems in various concentrations.

2d

61 013 M.C. PIETROGRANDE, P.A. BOREA, G. LODI, C. BIGHI, (Dep. Chem., Anal. Chem. Lab., Univ. Ferrara, Via L.Borsari 46, I-44100 Ferrara, Italy): Reversed-phase ion-pair systems to predict partition coefficients ofß-carbolines by HPLC and TLC. Chromatographia 23, 713-716 (1987). Study of a group of 17 ß-carbolines inreversed phase ion pairing TLC system in order to predict their log p values. TLC data found comparable for log pprediction in HPLC. Ion-pair TLC , log p prediction 2d, 23e

61 014 T. SHIMIZU*, Y. SUZUKI, C. INOSE, (*Dep. Chem., Fac. Educ., Gunma Univ., Aramaki, Maebashi 371, Japan): Chromatography of inorganic ions on polyethyleneimine cellulose layer in hydrochloric acid - ammoniumthiocyanate media. Chromatographia 23, 648-650 (1987). Survey of the TLC behavior of 58 inorganic ions onpolyethyleneimine (PEI) cellulose in hydrochloric acid - ammonium thiocyanate media. Investigation of the correlationbetween Rf values on PEI cellulose in the thiocyanate media and the paramagnetic moment of the rare earths (III), andof an odd-even fluctuation against the atomic number for the heavy rare earths (III). Demonstration of separation ofmany inorganic ions in the system. Inorganic ions , polyethyleneimine cellulose layer 2, 33a

61 015 D. SZÜCSOVA, Z. PERINA*, M. SARSUNOVA, V. VOSATKA, (*Forschungsinst. für Pharmazie und Biochemie, CSSR- 13060 Praha 3): Parameter der Adsoptionschromatographie. 5. Mittlg. Adsorptionsparameter potentiellerAntimycotica des Pyrimidin-Typs und ihre Korrelation zur biologischen Wirkung. Parameters of adsorptionchromatography, Part.5.: Adsorption parameters of potencial antimycotics of the pyrimidine type and their correlationwith biological activity. Pharmazie 42, 513-516 (1987). TLC of 19 fungicide pyrimidine derivatives on aluminium oxide(without binder) with 48 solvent systems (benzene - methanol, benzene - ethanol and chloroform - ethanol). Detectionwith 0.1% KMnO4 solution acidified 10% sulfuric acid (9:1). Pharmaceutical research , qualitative identification 2d, 29e

3. General techniques

61 018 J.S. CRIDLAND, (Dep. Pharm., Univ. Cape Town Med. Sch., Observatory, 7925 South Africa): Rapid application ofsamples to thin-layer chromatographic plates. Analyst 112, 1171- 1172 (1987). Description of a simple device forapplications of samples to TLC plates with the reproducibility comparable to that of standard methods and suitability forquantification. Several examples were given. TLC application device 3c

61 019 E. GATTAVECCHIA, D. TONELLI, P. BOSCO, (Fac. Farm., Univ. Bologna, 40127 Bologna, Italy): Evaluation of



61 019 E. GATTAVECCHIA, D. TONELLI, P. BOSCO, (Fac. Farm., Univ. Bologna, 40127 Bologna, Italy): Evaluation ofenzymatic kinetic parameters by thin-layer chromatography with radiometric detection. Anal. Chim. Acta 196,259-265 (1987). TLC of the products of enzyme reaction of lysine, ornithine and glutamic acid decarboxylases on silicawith acetone - 33% aqueous dimethyl amine - isopropanol 18:25:56. Evaluation by radioactivity linear analyserconnected to a computer based on a simple modification of the integrated Michaelis-Menten equation. Direct fit of theexperimental points to the equation by iterative techniques, the validity of which is tested on the simulated data. Quantitative analysis , enzyme reaction , radiometry , amino acids 3f, 18a

61 020 K. IMAEDA, K. OHSAWA, K. UCHIYAMA, SH. NAKAMURA, (Dep. Anal. Chem., Fac. Pharm. Sci., Hoshi Univ., Ebara,Shinagawa, Tokyo 142, Japan): Photoacoustic densitometry by fast laser beam scanning. Anal. Sci. 3, 11-15(1987). Description of a photoacoustic densitometry technique by laser beam scanning on a photoacoustic cell withoutchopping or modulation. Conversion and processing of the photoacoustic signals by microcomputer. The reproducibilityof measurement for 0.03 to 0.25 µg Malachite Green was 1.7-4.5% RSD. Photoacoustic densitometry , fast laser beam scanning 3f

61 021 W. JOST, H.E. HAUCK, (E. Merck, P.O. Box 4119, D-6100 Darmstadt, FRG): The use of hydrophilic modifiedsorbents in TLC. H. TRAITLER, A. STUDER, R.E. KAISER (eds): Instrumental HPTLC, Institute for Chromatography,Bad Dürkheim, FRG (1987), 241-253. Comparison of the polarities of the hydrophilic modified precoated plates, amino-,cyano-, diol-plates, with one another. Demonstration of the separations showing the application possibilities of theplates. Hydrophilic modified sorbents 3b

61 023 R.E. KAISER, R.I. RIEDER, (Institute for Chromatography, D-6702 Bad Dürkheim, P.O. Box 1141, FRG): Highpressure planar liquid chromatography (HPPLC). H. TRAITLER, A. STUDER, R.E. KAISER (eds): InstrumentalHPTLC, Institute for Chromatography, Bad Dürkheim, FRG (1987), 255- 264. Discussion of the concept, the principlesof application and practical details of planar liquid chromatography under high mobile phase pressure. HPPLC 3d

61 024 Y. LIU (Liu Yun), H. CHEN (Chen Hong), B. QIAN (Qian Baoying), Q. WANG (Wang Qinsun), (Dep. Chem. Eng.,Beijing Inst. Light Ind., Beijing, P.R. China): (A new reagent for enhancing and stabilizing fluorescence in TLCdetection of some pesticides. ) (Chinese). Chinese J. Chem. 4, 35-36 (1987) (Huaxue Tongbao). Use ofN,N-dimethyl-a-naphthylamine-5-sulfonyl chloride as a pre-chromatography derivatizing agent for some pesticides andherbicides. Spraying with tris(2-hydroxyethyl)-amine or paraffin oil after development. Detection by fluorodensitometryat 320 nm excitation and 500 nm emission. Spray reagent , pesticides 3e, 29

61 025 D. NUROK*, S. HABIBI-GOUDARZI, R. KLEYLE, (*Dep. Chem., Indiana Univ-Purdue Univ. at Indianapolis, P.O. Box647, Indianapolis, Indiana 46223, USA): Statistical approach to solvent selection as applied to two-dimensionalthin-layer chromatography. Anal. Chem. 59, 2424- 2428 (1987). Use of 2 functions as separation criteria forevaluating 28 solvent systems in the separation of each of 100 subsets of either 6 or 10 steroids. Discussion of theprobability of variation and the influencing factors. Solvent selection , two-dimensional TLC 3d, 13

61 027 S.K. POOLE, T.A. DEAN, C.F. POOLE*, (*Dep. Chem., Wayne State Univ., Detroit, MI 48202, USA): Preparation ofenvironmental samples for the determination of polycyclic aromatic hydrocarbons by thin-layerchromatography. J. Chromatogr. 400, 323-341 (1987). Discussion of extraction procedure, liquid-liquid distributionsystems, Sep-Pak cartridges, liquid- solid chromatography using silica, alumina and chemically modified silicapackings, macroreticular resins and gel permeation columns for the analysis of PAHs in environmental samples byTLC. TLC of 6 PAHs in air particulate and diesel particulate extracts on RP-18 silica with acetonnitrile - methanol -water 1:5:1. Identification by coincidence of retention between sample and standards. Quantification by densitometry. Environmental , quantitative analysis , densitometry , polycyclic aromatic hydrocarbons 3a, 5b

61 028 M. PROSEK, A. MEDJA, M. JELNIKAR, (Anal. Lab., Res. Dep., LEK, Celovska 135, YU-61000 Ljubljana), R.E.KAISER, (Inst. Chromatogr., D-6702 Bad Dürkheim, P.O. Box 1141, FRG): Quantitative evaluation of TLC withdigital scanning camera. H. TRAITLER, A. STUDER, R.E. KAISER (eds): Instrumental HPTLC, Institute forChromatography, Bad Dürkheim, FRG (1987), 321-329. Description of the hardware and software for quantitativeevaluation of TLC with image processing scanner taking the quantitative evaluation of (-)-a-bisabolol in essential oil asexample. Detection limit 200 ng. Standard deviation of measurements 3%. Quantitative analysis , digital scanning camera 3f

61 029 H.S. RATHORE*, H.A. KHAN, (*Chem. Section, Z.H. Coll. Engin. & Technol., Aligarh Muslim Univ., Aligarh 202 001,



61 029 H.S. RATHORE*, H.A. KHAN, (*Chem. Section, Z.H. Coll. Engin. & Technol., Aligarh Muslim Univ., Aligarh 202 001,India): Characterization of barium sulphate as a TLC material for the separation of plant carboxylic acids. Chromatographia 23, 432-434 (1987). Discussion of TLC of carboxylic acids on barium sulfate - calcium sulfate indifferent proportions with various solvents. Barium sulfate , carboxylic acids 3b, 11a

61 030 J.K. ROZYLO, I. MALINOWSKA, (Inst. Chem., M. Curie-Sklodowska Univ., Pl. M. Curie-Sklodowskiej 3, PL-20-031Lublin, Poland), D.Gwis-Chomicz, (Higher Pedagogical School, Zolnierska 13, Olsztyn, Poland): Chitin as stationaryphase in TLC. H. TRAITLER, A. STUDER, R.E. KAISER (eds): Instrumental HPTLC, Institute for Chromatography,Bad Dürkheim, FRG (1987), 349- 359. Investigations on the application of chitin as a TLC stationary phase, especiallya-chitin. Possibilities of obtaining mechanically and chromatographically stable precoated chitin layers. Application inthe separations of amino acids and dyes. Chitin , amino acids , dyes 3b, 18a, 30a

61 031 S.C. RUTAN*, C.B. MOTLEY, (Dep. Chem., Virginia Commonwealth Univ., Richmond, Virginia 23284- 0001, USA): Factor analysis and Kalman filter studies of severely overlapped amino acid derivatives in thin-layerchromatography. Anal. Chem., 59, 2045-2050 (1987). Investigation of the performance of several mathematicalapproaches for quantification of components in case of severe chromatographic and spectroscopic overlap.Demonstration by the separation of two amino acids, glycine and glutamine on silica with 1-butanol - acetone - aceticacid - water 7:7:2:4 followed by derivatization with O-phthaldialdehyde. Studies of 5 approaches based on factoranalysis and Kalman filtering methods with errors of 5.9% for glycine and - 6.4% for glutamine. Quantitative analysis , factor analysis , Kalman filtering method , amino acids 3f, 18a

61 032 Y. TSURUTA, K. KOHASHI, (*Fac. Pharm. Sci., Fukuyama Univ., Fukuyama, Japan 729-02): Sensitive derivatizationreagents for hydroxyl and amino compounds for thin-layer or high-performance liquid chromatography withfluorescence detection. Anal. Chim. Acta 192, 309-313 (1987). Description of 3 fluorescent derivatization reagentsfor compounds having hydroxyl and/or amino groups, which were stable at room temperature and condensestechiometrically with alcohols, amines and amino acids in the presence of alkali to give strongly fluorescentderivatives. Pre-chromatographic derivatization followed by TLC and HPLC. Derivatization reagents , hydroxyl and amino compounds 3e, 6, 17a, 18a

61 033 K. UCHIYAMA, (Dep. Anal. Chem., Hoshi Coll. Pharm., Tokyo, Japan, 142): (Application of the light scanningphotoacoustic densitometer to a chromatography system). (Japanese). Proc. Hoshi Coll. Pharm. 28, 51-57(1986) (Hoshi Yakka Daigaku Kiyo). Description of a light scanning photoacoustic densitometer for determination oforganic compounds separated on TLC plate with application examples. Discussion of the use of light emitting diodeand laser diode as the light source. Quantitative analysis , light scanning photoacoustic densitometer 3f

61 034 ZH. WANG (Wang Zheqing), (Shanghai Inst. Pharm. Ind., Shanghai, P.R. China): (A new common chromogenicreagent for thin-layer chromatography) (Chinese). Chinese J.Med.Ind. 18, 186-187 (1987) (Yiyao Gongye).Description of a new chromogenic reagent, which can be commonly used for TLC, prepared by mixing 12.0 g natriummolybdate, 7.5 mL phosporic acid, 25 mL sulfuric acid (98%) in 500 mL water. Description of the spraying procedure. Chromogenic reagent 3e

61 035 Z. WITKIEWICZ*, J. BLADEK, (Inst. Chem., Mil. Tech. Acad., 01 489 Warsaw, 49 Pol.): Overpressured thin-layerchromatography. J. Chomatogr. 373, 111-140 (1986). A review with 67 references on general properties of OPTLC,methods of developing chromatograms, application examples etc. Review , OPTLC 3d

61 036 Y. WU (Wu Youguang), (Jiangxi Med. Coll., Jiangxi, P.R. China): (A simple developing chamber for horizontalthin-layer chromatography) (Chinese). Chinese Anal. Chem. 15, 945-949 (1987) (Fenxi Huaxue). Description of adeveloping chamber for horizontal TLC. Demonstration of the separation of amino acids on cellulose - silica 5:2 withtwo solvent systems. The chamber is also suitable for continuous development. Developing chamber 3d, 18a

61 037 G. ZHANG (Zhang Guande), (Inst. Meteria Medica, Chinese Acad. Med. Sci., Beijing, P.R. China): (Brief introductionof some techniques in thin-layer chromatography). (Chinese). Chinese J. Chromatogr. 6, 24-26 (1988) (Sepu).Brief description of several techniques used in TLC of complex natural mixtures, including „hole-linear“ development,layer modifications, ionpair TLC, in situ derivatization, and others. Review , natural mixtures 3d

4. Special techniques



61 038 K.J. BARE, H. READ, (Res. Cent., Br. Pet. Co. PLC, Sunbury-on-Thames/Middlesex, UK TW16 7LN): Use of fastatom bombardment mass spectrometry to identify materials separated on high- performance thin-layerchromatography plate. Analyst 112, 433-436 (1987). Description of a technique for the identification of compoundsseparated by HPTLC using fast atom bombardment mass spectrometry (FAB-MS) without any extraction procedurebetween the two analysis steps. Discussion of the advantages of smaller samples, shorter analysis time and lessopportunities for contamination to occur than with conventional extraction methods. Identification of surfactants inmixed systems. Detection of amine antioxidants in gas oils at levels below 20 ng/µL. Qualitative identification , FAB-MS , surfactants 4e, 35a

61 039 R. M. BELCHAMBER, J. READ, J.D.M. ROBERTS, (BP Res. Cent., Sunbury on Thames/Middlesex, UK TW 16 7LN): Image processing of high-performance thin-layer chromatographic plates. J. Chromatogr. 395, 47-53 (1987).Description of an image processing system consisting of a solid state camera, a framestore, and a computer whichenables high-resolution images of the plates to be recorded and processed in a variety of ways. To correct fornonuniform illumination, the images of the developed plates are rationed with that of a blank plate. Software allows theimages to be enhanced and smoothed, and automatic quantitative analysis to be carried out. Image processing of TLC plates 4c

61 040 J.W. HOFSTRAAT, M. ENGELSMA, Q.J. VAN DE NESSE, U.A.T. BRINKMAN, C. GOOIJER, N.H. VELTHORST,(Dep. Gen. Anal. Chem. Free Univ., 1081 HV Amsterdam, Neth.): Coupling of narrow-bore liquid chromatographyto thin-layer chromatography. Part 2. Application of fluorescence-based spectroscopic techniques for off-linedetection. Anal. Chim. Acta 193, 193-207 (1987). Identification and determination of the compounds eluted from LC andcollected on a TLC plate by direct flurescence emission, fluorescence excitation emission spectra and fluorescenceline-narrowing spectroscopy with tetracene and benz(K)fluoranthene as model compounds. Discussion of the possibilityof widening the application of the technique. Detection limit, pg level for very selective narrow-line fluorescence. LC/TLC , fluorescence-based spectroscopy 4e, d

61 041 K. IWATANI, T. KADONO, Y. NAKAGAWA, (Shionogi Res. Lab., Osaka, Japan 553): (Direct coupling of thin-layerchromatography [TLC] with mass spectrometry [MS]. Direct TLC/MS using new sintered TLC plate) (Japanese).Jap. Mass Spectoscopy 34, 181-187 (1986) (Shitsuryo Bunseki). Description of a TLC/MS system for separation andmeasurement of mass spectra of nonvolatile and thermally unstable mixtures. Use of a TLC glass plate having manylinear grooves engraved on the rear which guide a cutter to cleave into strip to be set on a usual holder for massspectrometer. Qualitative identification , TLC/MS 4e

61 042 D.E. JÄNCHEN, (CAMAG, Sonnenmattstrasse 11, CH-4132 Muttenz, Switzerland): Direct coupling of HPLC andTLC. H. TRAITLER, A. STUDER, R.E. KAISER (eds): Instrumental HPTLC, Institute for Chromatography, BadDürkheim, FRG (1987), 185-192. Description of direct coupling of HPLC and TLC which are governed by differentmechanisms by using a device spraying the HPLC fractions onto the silica gel plate. Demonstration by threeapplication examples, e.g. separation and identification of 56 pesticide substances. Discussion of the scope ofpossibilities. Coupling of HPLC and TLC 4d, 29

61 043 J. LIAO (Liao Jie), Y. LU (Lu Yongquan), (Acad. Military Med. Sci., Beijing, P.R. China): (Studies on the chemicalconstituents of Pilosa asiabell, Codonopsis pilosula V. Study of the essential oil). J. Chinese Herb Med. 18,386-388 (1987) (Zhong Caoyao). TLC of palmitic formate on silica with benzene. Detection by spraying with vanillinreagent. Elution with diethyl ether. Identification by IR, MS. Pharmaceutical research , qualitative identification , essential oil , palmitic formate 4e, 15

61 044 M.S. STANLEY, K.L. BUSCH, (Dep. Chem. Indiana Univ., Bloomington, IN 47405 USA): Positive secondary-ionmass spectra and thin-layer chromatography / mass spectrometry of phenothiazine drugs. Anal. Chim. Acta194, 199-209 (1987). TLC on silica with methanol - NH3 200:3. Detection by UV 254 nm. Record of the secondary-ionmass spectra after extraction from the layer. Pharmaceutical research , TLC/MS 4e

61 045 P. SWANK-HILL, L.K. NEEDHAM, R.L. SCHNAAR, (Sch. Med., Johns Hopkins Univ., Baltimore, MD 21205 USA): Carbohydrate-specific cell adhesion directly to glycosphingolipids separated on thin-layer chromatographyplates. Anal. Biochem. 163, 27-35 (1987). TLC of various glycosphingolipids on silica coated with a thin film of poly(iso-Bu methacrylate). Plate placed in a chamber containing metabolically radiolabelled cells, sealed and centrifuged tobring the cells into contact with the surface of the plate. Development of the cell adhesion blocked by a solution ofN-acetyl-glycosamine in various solvents. Detection by autoradiography. Detection limit, 8 pmol. Glycosphingolipids 4e, 11c



5. Hydrocarbons and halogen derivatives

61 047 G.J. BURRELL, R.J. HURTUBISE, (Chemistry Dept., Univ. of Wyoming, Laramie, Wyoming 82071, USA): Solidsurface luminescence interactions of nitrogen heterocycles adsorbed on silica gel chromatoplates submergedin chloroform/n-hexane solvents. Anal. Chem. 60, 564-508 (1988). TLC of phenanthrene, naphthalene,fluoranthene, triphenylene on silica with n-hexane. Visualization under UV after submerging in liquid nitrogen.

5b

61 048 K. TANAKA, K. SUGAWARA, K. MASUDA, (Coll. Eng., Kanto-Gakuin Univ., Kanazawa, Yakohama 236, Japan): Determination of benzo(a)pyrene in soil by synchronous fluorometry. Anal. Sci. 3, 89-90 (1987).Two-dimensional TLC on silica (1st direction) and 26% acetylated cellulose (2nd direction) with solvent systemsreported earlier. Quantification by synchronous fluorometry. Comparison of the results with those using conventionalmethods. Environmental , quantitative analysis , densitometry , PAHs , benzo(a)pyrene 5b

61 049 F.C. TRUSELL, (Consulting Anal. Chemist, 6910 South Prince Way, Littleton, Colorado 80120, USA): Petroleum. Anal. Chem. 59, 252R-280R (1987). A review on TLC application in petroleum analysis with many references. Alsoother techniques. Review , petroleum 5

6. Alcohols

61 051 M. NISHIKATA,(Cent. Res. Div., School Dentis.Hokkaido Univ., Sapporo 060, Japan): Separation of ß-aminoalcohols as their dansyl derivatives by thin-layer chromatography on a polyamide layer sheet. J. Chromatogr.408, 449-452 (1987). Two-dimensional TLC on polyamide with 1) 5% acetic acid in water and 2) benzene - heptane -acetic acid 7:2:1. Detection by UV. Qualitative identification , ß-amino alcohols 6

8. Substances containing heterocyclic oxygen

61 052 S. BLADT, H. WAGNER, (Inst. Pharmaz. Biologie, Univ. München, Karlstr. 29, D-8000 München, F.R.G.): 1)Cumarindrogen - Erste Mitteilung - Qualitätsprüfung der Umcka-Droge und ihrer Zubereitungen. (Coumarindrugs - quality testing of the umcka drug and its preparations.) Dtsch. Apoth. Ztg. 128, 292-296 (1988). TLC ofumckalin, scopoletin umckalinic acid, umckalin-7-O-glucoside on silica with ethyl acetate - formic acid - acetic acid -water 100:11:11:26. Detection under UV 365 nm after spraying with KOH solution; separation of umckalin andscopoletin with toluene - ether 50:50 saturated with 10% aqueous acetic acid. Pharmaceutical research , qualitative identification 8b

61 053 L.E. CLARK*, E. WOLLENWEBER, W.D. CLARK, (*Dep. of Botany, Arizona State Univ., Tempe, AZ 85287, USA): Flavonoid similarities among five species Isocoma. Biochemical Systematics and Ecology 15, 639-641 (1987).TLC of flavonoids on polyamide. Visualization under UV at 366 nm both before and after spraying with natural dyereagent A/ß-amino-ethylester of diphenyl-boric acid. Agricultural , qualitative identification 8a

61 054 EL-GENGAIHI*, M.S. KARAWYA, G. WASSEL, N. IBRAHIM, (*Pharm. Sci. Lab., National Research Centre, Cairo,Egypt): Investigation of flavonoids of Abrus precatorius L. Herba Hungarica 27, 27-33 (1988). TLC of theflavonoids 5,7,4-trihydrixy-flavane-glycoside, 7,4-dihydroxy- flavanol-diglycoside and taxifolin-3-glycoside on polyamidewith ethanol - water 3:2. Detection with 5% aluminium chloride in ethanol. TLC of the aglycones and sugar moieties onsilica impregnated with phosphat buffer (pH = 5) with n-butanol - acetone - phosphate buffer 40:40:10. Detection byspraying with aniline oxalate reagent. Agricultural , qualitative identification 8a

61 055 P. GHOSH, P. SIL, S. THAKUR*, (*Natural Products Lab., Dep. Chem., Univ. Burdwan, Burdwan 713104, India): Spray reagent for the detection of coumarins and flavonoids on thin-layer plates. J. Chromatogr. 403, 285-287(1987). Presentation of a spray reagent, chlorosulphonic acid - chloroform 4:1, for the detection of coumarins andflavonoids on silica. Detection limits, less than 1 µg for most of the 28 test compounds. Qualitative identification , spray reagent , coumarins , flavonoids 8a

61 056 K. GLUCHOFF-FLASSON, M. JAY, (Lab. de Biol. Micromoléculaire et Phytochimie, Université Lyon 1, 43 bd. du 11



61 056 K. GLUCHOFF-FLASSON, M. JAY, (Lab. de Biol. Micromoléculaire et Phytochimie, Université Lyon 1, 43 bd. du 11Novembre 1918, F-69622 Villeurbanne Cedex, France): Discriminative flavonoid patterns within a preliminarycollection of the Setaria italica specific complex. Biochemical Systematics and Ecology 15, 581-586 (1987).Two-dimensional TLC of flavonoids on polyamide with toluene - methanol - MEK - n-butanol 30:20:15:0.3 (1stdirection), water - n-butanol - acetone - dioxane 110:15:10:5 (2nd direction). Agricultural , qualitative identification 8a

61 057 J.J. HOFFMANN*, H. PUNNAPAYAK, S.D. JOLAD, R.B. BATES, F.A. CAMON, (*Bioresources Research Facility, 250East Valencia Road, Tucson, Arizona 85706, USA): Bioconversion of grindelic acid into 3a-hydroxygrindelicacid. Journal of Natural Products 51, 125-128 (1988). TLC of 3a-hydroxygrindelic acid andmethyl-3a-hydroxygrindelate on silica with n-hexane - ethyl acetate - acetic acid 70:30:2. Visualization by spraying withp-anisaldehyde - acetic acid - sulfuric acid 1:120:1, followed by heating. Method used for monitoring a fermentationprocess. Clinical chemistry research , quality control , qualitative identification 8b

61 058 Y. LU (Lu Yunru), L. ZHANG (Zhang Lanzhen), A. SHAO (Shao Aixin), (Beijing Trad. Chinese Med. Coll., Beijing, P.R.China): (Quantitative analysis for three main isoflavonoids in Chinese medicine She Gan by thin-layerchromatography) (Chinese). J.Chinese Herb Med. 7, 275-279 (1987) (Zhongcaoyao). TLC of tectoridin, tectorigeninand irigenin on silica with chloroform - methanol - formic acid 8:2:0.1, chloroform - acetic acid 10:0.2. Quantification bydensitometry at 271 nm. RSD 2.09 ± 0.92%. Pharmaceutical research , quantitative analysis , densitometry , isoflavonoids 8a, 32e

61 059 S.B. MAHATO*, K.A.I. SIDDIQUI, G. BHATTACHARYA, (*Indian Inst. of Chemical Biology, Jadavpur, Calcutta-700032, India): Structure and stereochemistry of phaseolinic acid: a new acid from Macrophomina phaseolina. Journal of Natural Products 50, 245-247 (1987). TLC on silica with benzene - methanol - 90:10. Visualization under UV. Agricultural , qualitative identification 8b

61 060 R. MATA*, F. CALZADA, M.R. GARCIA, M.T. REGUERO, (*Dep. de Farmacia, Cindad Universitaria, Coyoacán 04510,Mexico DF): Chemical studies on mexican plants used in traditional medicine, III. New 4-phenylcoumarins fromExostema caribaeum. J. of Natural Products 50, 866-871 (1987). TLC after extraction of5-O-ß-D-galactosyl-7-methoxy-3',4'-dihydroxy-4-phenylcoumarin, 7,4',5'-trihydroxy-4-phenyl-5,2'-oxido-coumarin and7,4'-dimethoxy-5'-hydroxy- 4-phenyl-5,2'-oxido-coumarin on silica with ethyl acetate - methanol - water 12:1:3.Visualization by UV or by spraying with 0.4N sulfuric acid or anisaldehyde reagent. Agricultural , qualitative identification 8b

61 061 B. MEURER, R. WIERMANN, D. STRACK, (Univ. Köln, Bot. Inst., Gyrhofstrasse 15, D-5000 Köln 41): Phenylpropanoid patterns in Fagales pollen and their phylogenetic relevance. Phytochemistry 27, 823-828(1988). TLC of quercetin 3-O-glucosylgalactoside and various hydroxycinnamic acid - spermidine amides on cellulosewith chloroform - acetic acid - water 3:2, water satd., n-butanol - water - acetic acid - 6:2:1 and on polyamide with water- MEK - methanol - acetyl acetone 13:3:3:1. Detection under UV and with 4-nitro-aniline and Dragendorff reagent. AlsoHPLC on RP-18. Qualitative identification 8

61 062 K.W. NICHOLLS*, B.A. BOHM, (*Botany Depart., The Univ. of British Columbia, Vancouver, Brit. Col. V6T 2B1,Canada): Flavonoids of Lupinus texensis and Lupinus subcarnosus. Biochemical Systematics and Ecology 15,571-573 (1987). Identification of flavonoids by two-dimensional TLC on polyamide with water - n-butanol - acetone -dioxane 70:15:10:5 (first direction) and benzene - MEK - methanol - water 55:23:20:2 (second direction). Examinationunder UV 366 nm before and after spraying with aminoethyl diphenyl-borate. Agricultural , qualitative identification 8a

61 063 P.M. PETERSON*, L.H. RIESEBERG, (*Dep. of Botany and Ownbey Herbarium, Washington State University,Pullman, WA 99164, USA): Flavonoids of the annual Muhlenbergia. Biochemical Systematics and Ecology 15,647-652 (1987). Two-dimensional TLC on polyamide with water - n-butanol - acetone - 1,4-dioxane 70:15:10:5 andbenzene - MEK - methanol - water 55:22:20:3. Detection by spraying with 0.1 % aminoethyl - diphenylborate inmethanol - water 1:1. TLC of O-glucosides after acid hydrolysis (with 6 % HCl and heating for 40 min. at 100°C) oncellulose with ethyl acetate - pyridine - water 12:5:4. Agricultural , qualitative identification 8a

61 064 O. POTTERAT, H. STOECKLI-EVANS, J.D. MSONTHI, K.HOSTETTMANN, (Inst. de Pharmacogn. et Phytochimie,



61 064 O. POTTERAT, H. STOECKLI-EVANS, J.D. MSONTHI, K.HOSTETTMANN, (Inst. de Pharmacogn. et Phytochimie,Univ. de Lausanne, 2, rue Vuillermet, CH-1005 Lausanne): New antifungal naphthoxirene derivatives and theirglucosides from Sesamum angolense welw. Helv.Chim. Acta 70, 1551-1557 (1987). TLC of new naphthoxirenederivatives on silica with chloroform - methanol 85:15 or chloroform - methanol - water 65:35:5 and HPTLC on RP-8with different methanol/water mixtures. Detection under UV 254 nm. The fungicidal activity was determined with a TLCbioassay using Cladosporium cucumerinum. Qualitative identification , natural products isolation 8

61 065 A. RATHORE*, S.C. SHARMA, J.S. TANDON, (*Central Drug Research Inst., Lucknow, 226001, India): A newmethoxylated ß-hydroxychalcone from Polygonum nepalense. Journal of Natural Products 50, 357-359 (1987).TLC of quercetin-3-O-rhamnobioside, hyperoside, luteolin-6-C- glucoside on silica with chloroform - ethyl acetate 19:1.Visualization under UV after spraying with 1% ceric sulfate in 2N sulfuric acid. Agricultural , qualitative identification 8a

61 066 C. SCHEELE*, E. WOLLENWEBER, F.J. ARRIAGA-GINER, (*Inst. für Botanik der TH Darmstadt, Schnittspahnstr. 3,D-6100 Darmstadt, F.R.G.): New flavonoids from Cheilanthoid ferus. Journal of Natural Products 50, 181-187(1987). TLC of flavonoids on polyamide with toluene - petrol ether (bp. 100-140°C) - MEK - methanol 30:90:2:1.5 or12:6:2:1 and toluene - methanol - MEK 12:5:3. Visualization at 366 nm before and after spraying with natural dyereagent A. TLC of terpenoids on silica with toluene - MEK 9:1 and toluene - dioxane - acetic acid 18:5:1. Visualizationby spraying with MnCl2 reagent and heating at 130 °C. Agricultural , qualitative identification 8a, 15a

61 067 GY. TURIAN*, L. FARKAS-TOMPA, (Research Inst. for Medicinal Plants, Budakalász, Hungary): Problems insolvedin standardization of flavonoids in crude drugs and extracts from medicinal plants. Acta Pharm. Hungarica 57,193-198 (1987). HPTLC of rutin, vitexin-4'- rhamnoside, hyperoside, vitexin and apigenin-7-glucoside on silica withethyl acetate - formic acid - acetic acid - water 100:11:11:28 for glycosides, with toluene - ethyl acetate - acetic acid -water 30:50:16:4 for aglycones. Detection under UV 254 and 366 nm and spraying with 1% AlCl3 methanolic solution.Densitometry by absorbance at 400 nm. Clinical chemistry research , densitometry 8a

61 068 A. URZUA*, R. RODRIGUEZ, B. CASSELS, (*Depart. de Quimica, Facultad de Ciencia, Univ. de Santiago de Chile,Casilla 5659, Santiago-2, Chile): Fate of ingested aristolochic acids in Battus archidamas. BiochemicalSystematics and Ecology 15, 687-689 (1987). TLC of aristolochic acids on silica with chloroform - methanol 85:15 or2:1. Quantification by spectrophotometry at 254 nm. Agricultural , quantitative analysis , qualitative identification 8b

61 069 S. YU, N. FANG, T.J. MABRY, (Depart. of Botany, Univ. of Texas at Austin, Austin TX 78713, USA): Flavonoids fromGymnosperma glutinosum. Phytochemistry 27, 171-177 (1988). Separation of complex mixtures of highlyoxygenated flavonols by TLC on cellulose with t-butanol - acetic acid - water 3:1:1 or 15% aq. acetic acid. Detectionwith natural dye reagent A and under UV 254/366 nm. Rf values and colors of numerous flavonoids are given. Alsocolumn chromatography for the preparative separations. Qualitative identification , natural products isolation 8a

9. Oxo compounds, ethers and epoxides

61 070 ZH. JIANG (Jiang Zhihau), M. HAN (Han Meifang), Y. CHENG (Cheng Yiqin), (Shanghai Inst. Chem.& Tech. Prod.forDaily Use, Shanghai, P.R. China): (Determination of vanillin by thin-layer chromatography). (Chinese). ChineseJ. Chem. World 29, 24-27 (1988) (Huaxue Shijie). TLC on silica with chloroform - methanol 98:2. Determination bydensitometry at 315 nm. Food analysis , quantitative analysis , densitometry , vanillin 9

61 071 K.J. MELKSHAM*, S. AXELSEN, (*Agr. Chem. Branch, Queensland Dep. Prim. Ind., Meiers Road, Indooroopilly,Queensland 4068, Australia): Preparation, identification, separation gas-liquid and thin-layer chromatography ofthe 2,4-dinitrophenylhydrazone derivatives of acetone and acrylaldehyde. J. Chromatogr. 396, 399-403 (1987).TLC on silica with toluene. Detection by the yellow-orange color of the compounds. Detection limit 0.1 µg. Agricultural , qualitative identification , 2 , 4-dinitrophenylhydrazone derivatives of acetone and acrylaldehyde 9, 29f

61 072 E. TYIHAK, G. GULLNER*, (*Plant Protection Inst., Hungarian Acad. of Sci., H-1525 Budapest, P.O. Box 102,



61 072 E. TYIHAK, G. GULLNER*, (*Plant Protection Inst., Hungarian Acad. of Sci., H-1525 Budapest, P.O. Box 102,Hungary): Endrogenous formaldehyde levels in red peper varieties. Proc. Scientific InternationalTechnical-Development Symposium on Hungarian Paprika, September 1987. Kalocsa, p. 499. OPTLC analysis offormaldehyde using methylated lysine derivatives chromatographed on silica with dichloromethan - chloroform 65:35.Densitometry. Agricultural , densitometry 9

10. Carbohydrates

61 073 A. LIPTAK*, J. KEREKGYARTO, L. SZABO, J. HARANGI, (*Kossuth Lajos Univ., Inst. of Biochemistry, H-4010Debrecen, Hungary): Hydrogenolysis of dioxane-type benzylidene acetals fused to furanoide rings. Synthesis of3-O-methyl-L-xylose. Acta Chim. 124, 315-321 (1987). Elucidation of the structures of synthesized compounds by TLC(and 13C-NMR spectroscopy). TLC on silica with dichloromethane - methanol 95:5, dichloromethane - ethyl acetate9:1, dichloromethane - acetone 9:1, dichloromethane - methanol 85:15. Detection by charring with 50% sulfuric acid orunder UV. Quality control , qualitative identification 10a

61 074 M.A. SMITH*, J.V. JACOBSEN, H. KENDE, (MSU-DOE Plant Res.Lab., Michigen State Univ., East Lansing, MI48824): Amylase acivity and growth in internodes of deep-water rice. Planta 172, 114-120 (1987). TLC ofglucose, fructose, maltose on silica with isopropanol - acetone - water 45:30:25. Detection reagent: 4 g diphenylamine,20 mL aniline, 30 mL phosphoric acid in 200 mL acetone. After spraying, heating for 15 min at 100 °C. Agricultural , qualitative identification 10a

61 075 CH. ZHANG (Zhang Chunling), Y. ZHANG, (Zhang Yingshan), (Inst. Cardiovasc. Dis., Chin. Acad. Sci., Beijing, P.R.China): (Thin-layer chromatography of unsaturated sulfated disaccharides). (Chinese). Chinese J. Adv.inBiochem.& Biophys. 2, 64-66 (1987) (Shengwu Huaxue Yu Shengwu Wuli Jinzhan). TLC on cellulose with n-butanol -acetic acid - 2M NH4OH 2:3:1. Detection under UV at 260 nm. Identification by comparison of Rf values to standards. Qualitative identification , sulfated disaccharides 10b

11. Organic acids and lipids

61 076 J.G. ALVAREZ, J. LUDMIR, B.T. STOREY, J.C. TOUCHSTONE, (Dept. Obstetrics etc., Univ. Pennsylvania,Philadelphia, Pennsylvania 19104): Isolation of cholesteryl palmitate from human term amniotic fluid. J. LiquidChrom. 10, 1291-1298 (1987). TLC of amniotic fluid and phospholipid standards on silica with chloroform - ethanol -triethylamine - water 30:34:30:8. Detection: after drying at 170 °C for 2 min. spraying with 10 % solution of coppersulfate in 8 % H3PO4, and 5 % copper acetate in 8 % H3PO4 and heating again at 110 °C for 5 min.; after inspection,heating up to 170 °-180 °C.

11c, 32f

61 077 L. COLAROW, B. PUGIN, D. WULLIEMIER, (Nestlé Research Center, Nestec Ltd., Vers-chez-les Blanc, CH-1000Lausanne 26, Switzerland): The use of film negatives for laser densitometry of complex lipids in combinationwith planar chromatography and fluorescence detection. H. TRAITLER, A. STUDER, R.E. KAISER (eds):Instrumental HPTLC, Institute for Chromatography, Bad Dürkheim, FRG (1987), 101-109. TLC of phospholipids andglycolipids on silica with chloroform - methanol - triethylamine - water 30:35:34:8 and chloroform - methanol - 0.25%calcium chloride 60:35:8, resp. Detection by dipping in TNS reagent and photographing on film negatives.Measurement by laser scanning in transmittance mode. Detection limit, 25 ng. Quantitative analysis , densitometry , phospholipids , glycolipids 11c

61 078 N. FRANJIC*, R. NAUMSKI, A. CASTEK, K. KLJAIC, (SOUR „Pliva“, Istrazivacki Inst., Zagreb): The effect of thebiotin on the biosnthesis of lipids and composition of unpolar lipid mixtures from penicillin treatedBrevibacterium divaricatum NRRL 2311. Acta Pharm. Jugoslavica 37, 87-95 (1987). TLC of lipid compounds onsilica with petrol ether - ether - acetic acid 85:15:12. Staining by spraying with molybdate- perchloric acid reagent.Densitometry by absorbance. Pharmaceutical research , densitometry 11c

61 079 ZH. GU (Gu Zhiping)*, L. HE (He Liyi), (*Zibo People’s Pharm. Factory, Zibo, Shandong, P.R. China): (Determinationof organic acids in Guangdong antivenom drug by thin-layer chromatography. ) (Chinese). J. Chinese Herb Med.18, 399-401 (1987) (Zhongcaoyao). TLC on silica with chloroform - ether - butyl formiate - formic acid - methanol5:1.5:1:1:0.25. Detection by heating at 120 °C for 2 h and spraying with 0.04 % bromophenol blue in water.Quantification by densitometry at 430 nm. Pharmaceutical research , quantitative analysis , densitometry , organic acids 11a



61 080 Y. KIMURA*, H. OKUDA, T. OKUDA, T. HATANO, S. ARICHI, (*2nd Dep. of Medical Biochemistry, School of Medicine,Ehime Univers., Shigenobu-cho, Onsen-gun, Ehime 791-02, Japan): Studies on the activities of tannins and relatedcompounds, X. Effects of caffeetannins and related compounds on arachidonate metabolism in humanpolymorphonuclear leukocytes. Journal of Natural Products 50, 392-399 (1987). TLC of arachidonic acid andmetabolites on silica with ethyl acetate - 2,2,4-trimethylpentane - acetic acid - water 100:50:20:100, upper phase andchloroform - methanol - acetic acid - water 13:12:1.5:1.2. Detection by autoradiography. Agricultural , qualitative identification , comparison of methods 11a

61 081 M.S. KREHULA, M. TAJIC, (Departm. of Chemistry, Univ. of Zagreb, Fac. of Pharm.and Biochemistry, 41000 Zagreb,A.Kovacica 1): Vitamin D and its metabolites in the pollen of Pine. Part. 5.: Steroid hormones in the pollen of Pinespecies. Pharmazie 42, 471-472 (1987). TLC of lipids on silica with ethyl acetate - hexane 1:1. Zones corresponding tothe standards rechromatographed twice more with ethyl acetate - hexane 1:1 and chloroform - ethanol - water 183:16:1. Pharmaceutical research , qualitative identification 11c

61 082 C. LERAY, X. PELLETIER, S. HEMMENDINGER, J.P. CAZENAVE, (Cent. Neurochim., C.N.R.S., 67037 Strasbourg,France): Thin-layer chromatography of human platelet phosholipids with fatty acid analysis. J. Chromatogr.420, 411-416 (1987). TLC of phospholipids and fatty acids on boric acid-impregnated silica with chloroform - ethanol -water - triethyl amine 30:35:6:35. Detection of phospholipids by spraying with a solution of 5 mg primulin in 100 mLacetone - water 4:1 and examining under UV; detection of fatty acids by exposing to iodine vapour. Determination offatty acids by GC after elution. Clinical chemistry research , quantitative analysis , phospholipids , fatty acids 11c, 4d

61 083 SH. LI (Li Shiqing), (Guizhou Inst. Metal. Prot., Zunyi, Guizhou, P.R. China): (Screening of low erucic rape strain bythin-layer chromatography). (Chinese). Chinese J. Chromatogr. 6, 59-60 (1988) (Sepu). TLC of erucic acid on silicawith toluene - hexane 95:5. Detection by spraying with 0.05% dichlorofluorescein. Semiquantification by comparison tostandards. Agricultural , quantitative analysis , erucic acid 11a

61 084 L. LIU (Liu Li), D. XU (Xu Desheng), D. XIE (Xie Delong), (Shanghai Shuguang Hospital, Shanghai, P.R. China): (Determination of cholic acid and deoxycholic acid in fast-acting cold capsules by thin-layer chromatography. )(Chinese). Chinese J. Med. Ind. 18, 361-362 (1987) (Yiyao Gongye). TLC on silica with hexane - ethyl acetate - aceticacid - methanol 75:400:12.5:12.5. Detection by spraying with 15% sulfuric acid - ammonium hydrogen sulfate andheating at 110 °C for 10 min. Quantification by densitometry at 285 nm. RSD 0.54 %. Clinical chemistry research , quantitative analysis , densitometry , cholic acid , deoxycholic acid 11a

61 085 A. MINENKO, G. KISELEV, E. TULKOVA, P. OEHME*, (*Inst. of Drug Research, DDR-1136 Berlin, Alfred-Kovalke-Str.4): Nicotinic stimulation of polyphosphoinositide turnover in rat adrenal medulla slices. Pharmazie42, 341-344 (1987). TLC of phospholipids on 1% potassium oxalate-impregnated silica with chloroform - acetone -methanol - acetic acid - water 40:15:13:12:8. Location by autoradiography or by iodine staining. Quantification by liquidscintillation after elution. Pharmaceutical research , qualitative identification 11c

61 086 A.H. REINE, S.J. STEGINK, (Southern Reg. Research Center, Agricult. Research Serv., US Dep. of Agricult., P.O. Box19687, New Orleans, LA 70179, USA): Qualitative and quantitative thin-layer chromatographic analysis of polarlipids from leaves of glanded and glandless cotton genotypes. J. Chromatogr. 437, 211-219 (1988).Characterization of polar lipids from leaves of 7 cotton varieties by two-dimensional TLC on silica with 1. chloroform -n-propanol - methanol - acetic acid 60:30:10:2 and, after reactivation of the plate at 110°C for 1h, with 2. chloroform -methanol - 7 M NH3 65:35:5. Detection with cupric acetate. Densitometry at 350 nm. Also prep. TLC on silica withchloroform - acetone - methyl acetate - n-propanol - acetic acid 30:40:30:15:0.5. Visualization of the lipids with2,7-dichlorofluorescein. Elution from the plate with chloroform - metanol 1:1. Agricultural , densitometry , preparative TLC 11c

61 087 A. STUDER, P. SACHE, H. TRAITLER, (Nestlé Res.Cent., Nestec Ltd. Vers-chez-les Blanc, CH-1000 Lausanne 26,Switzerland): Optimization of resolution on very thin-layers in anticircular planar chromatography. H.TRAITLER, A. STUDER, R.E. KAISER (eds): Instrumental HPTLC, Institute for Chromatography, Bad Dürkheim, FRG(1987), 379-387. Anticircular TLC of impurities of Jojoba wax esters and decomposed triglycerides on very thin silicalayer with petrol ether - ether 6:4. Detection by dipping in manganese chloride - sulfuric acid reagent and heating at150 °C for 50 sec. Quantification by densitometry at 546 nm. Very thin layers , lipids 11c

61 088 K. TSUNAMOTO*, SH. TODO, (*Dep. Pediatries, Kyoto Prefectural Univ. Med. Kawaramachi-Hirokoji, Kamikyo-Ku,



61 088 K. TSUNAMOTO*, SH. TODO, (*Dep. Pediatries, Kyoto Prefectural Univ. Med. Kawaramachi-Hirokoji, Kamikyo-Ku,Kyoto 602, Japan): Separation of prostaglandins and thromboxane by two- dimensional thin-layerchromatography. J. Chromatogr. 417, 414-419 (1987). Two-dimensional TLC of prostaglandins, thromboxane andarachidonic acid with ethyl acetate - iso-octane - acetic acid - water 11:5:2:10 and chloroform - methanol - acetic acid90:8:6. Detection by spraying with 10% phophomolybdic acid in ethanol. Discussion of the chromatographic conditions. Pharmaceutical research , prostaglandins , thromboxane , arachidonic acid 11b

61 089 H. ZHANG (Zhang Huidi), SH. HAN (Han Shitian), J. ZO (Zo Jianjun), Y. SU (Su Yibing), T. WU (Wu Tongxing),Y.CHEN (Chen Yaozu), (Dep. Chem., Lanzhou Univ., Lanzhou, P.R. China): (Determination of10-hydroxyl-D2-decanoic acid in royal jelly by thin-layer chromatography. ) (Chinese). Chinese J. Pharm.Anal. 7,338-340 (1987) (Yaowu Fenxi Zazhi). TLC on silica with ethyl acetate - petrol ether - acetic acid 8:2:0.3. Quantificationby densitometry at 210 nm. Comparison of the results with those from other methods. Pharmaceutical research , quantitative analysis , densitometry , 10-hydroxyl-D2-decanoic acid 11a, 32e

61 090 G. ZHOU (Zhou Guilan), SH. CAO (Cao Shuying), (Changchun Inst. Appl. Chem., Changchun, P.R. China): (Determination of monoglycerine stearate by thin-layer chromatography. ) (Chinese). Chinese Anal. Chem. 15,748-750 (1987) (Fenxi Huaxue). TLC of mono-, di-, tri- glycerol stearates on silica with cyclohexane - chloroform - THF85:25:20. Detection by exposing to iodine vapor. Quantification by densitometry at 420 nm. RSD 1.2-2.7% Quantitative analysis , densitometry , monoglycerine stearate 11c

13. Steroids

61 091 ANONYMOUS: Changes in methods - mammalian feces thin layer chromatographic method for coprostanol. J.A.O.A.C. 71, 226 (1988). TLC of coprostanol on silica with ether - heptane 55:45 after equilibration. Before sampleapplication, plate is immersed in 5 % phosphomolybdic acid in ethanol and air dried. After chromatography, heating for20 min at 120 °C.

13a

61 092 J. BASCOUL, D. DESPLAN, N. DOMERGUE, A. CRASTES DE PAULET, (INSERM U. 58, 60 Rue de Navacells,F-34090 Montpellier, France): Evaluation of oxysterols in biological samples and foods by TLC. H. TRAITLER,A. STUDER, R.E. KAISER (eds): Instrumental HPTLC, Institute for Chromatography, Bad Dürkheim, FRG (1987),35-41. TLC on silica with ether - benzene - cyclohexane 90:10:1. Detection by spraying with an aqueous solution ofsaturated copper acetate - orthophosphorous acid 5:5 and heating. Evaluation by densitometry using 7ß-hydroxydehydroepiandrosterone and 3a-11ß-20ß-trihydroxy(5ß) pregnane as internal standards. Sensitivity, 200 ng. Clinical chemistry research , food analysis , quantitative analysis , densitometry , oxysterols 13c

61 093 X. GAO (Gao Xiangjun), H. LIU (Liu Hanfang), B. Zhang (Zhang Bochong), (Gansu Inst. Drug Cont., Lanzhou, Gansu,P.R. China): (Analysis of cholic acid in Dannanxing, Arisaema cum bile, and its compound preparations). (Chinese). Bulletin Chinese Trad. Med. 12, 350-352 (1987) (Zhongyao Tongbao). TLC on silica with cyclohexane -ethyl acetate - acetic acid 2:8:1.5. Quantification by densitometry at 365 nm. Detection limit < 1 µg. Pharmaceutical research , quantitative analysis , densitometry , cholic acid 13d

61 094 K. MORRIS, J. SHERMA, B. FRIED, (Dept. Chem., Lafayette College, Easton, Pennsylvania 18042, USA): Determination of cholesterol in egg yolk by high performance TLC with densitometry. J. Liquid Chrom. 10,1277-1290 (1987). TLC on silica, Ag+ impregnated silica with e.g. isopropyl ether - acetic acid 96:4, then with petrolether - acetic acid 90:10:1. Also chromatography on RP-18 silica. Derivatization with phosphomolybdic acid reagent, forquantification with copper acetate - H3PO4 reagent. Quantification by densitometry.

13c

61 095 J.TH. SCHILLER, V. GRAEF, S.W. GOLF, (Zentrum für Klin. Chem. und Pathobiochem.des Klinikums der Justus LiebigUniv. Giessen, D-6300 Giessen, FRG), W.FUNK,(FH Giessen, Wiesenstrasse 14, D-6300 Giessen, FRG): Quantitative HPTLC determination of D-3-oxo steroids and their reduced 5-a,ß-metabolits. H. TRAITLER, A.STUDER, R.E. KAISER (eds): Instrumental HPTLC, Institute for Chromatography, Bad Dürkheim, FRG (1987),369-378. TLC of androgenes, mineralocorticoides, glycocorticoides and gestagenes on silica with 3 different solventsystems. Detection by dipping in 4-dimethylaminobenzaldehyde/cinnanic-aldehyde/H2SO4 reagent. Detection limit, 500pg - 1 ng. Quantification by fluorodensitometry. Clinical chemistry research , quantitative analysis , densitometry , D-3-oxo steroids , androgenes ,mineralocorticoides , glucocorticoides , gestagenes

13

61 096 F. SENATORE*, A. DINI, R. CERRI, O. SCHETTINO, (*Dip. di Chimica delle Sostanze Naturali, Univ. di Napoli, Via



61 096 F. SENATORE*, A. DINI, R. CERRI, O. SCHETTINO, (*Dip. di Chimica delle Sostanze Naturali, Univ. di Napoli, ViaL.Rodino 22, I-80138 Napoli, Italy): Chemical constituents of some Tricholomataceae. Biochemical Systematicsand Ecology 15, 639-641 (1987). TLC on argentated silica with benzene - hexane 1:1, two runs. Agricultural , qualitative identification 13c

61 097 I.D. WILSON*, S. LEWIS, (*Drug Metab. Sec., Imperial Chem. Ind. PLC, Mereside, Alderley Park, Macclesfield,Cheshire SK 10 4TG, U.K.): Separation of ecdysteroids by high-performance thin-layer chromatography usingautomated multiple development. J. Chromatogr. 408, 445-448 (1987). TLC of ecdysone, makisterone A,polypodine B, ponasterone A, ponasterone C and polypodine B 2-cinnamate on silica with various solvent systemsbased on hexane - dichloromethane and dichloromethane - methanol using AMD. Detection by visualizing under UV254 nm; densitometry by absorbance at 260 nm. Comparison of the results with those from manual multipledevelopment and single development. Qualitative identification , comparison of methods , ecdysteroids , AMD 13e, 3d

14. Steroid glycosides, saponins and other terpenoid glycosides

61 098 N. CHAURASIA, M. WICHTL*, (*Inst. für Pharmazeutische Biologie, Phillips-Univ., Deutschhausstr. 17, D-3550Marburg/Lahn, F.R.G.): Sterols and steryl glycosides from Urtica dioica. J. Natural Products 50, 881-885 (1987).TLC of 6 steryl derivatives isolated from the roots of Urtica on silica with chloroform - methanol 93:7. Detection byspraying with vanillin-phosphorous acid reagent or 50 % H2SO4 in ethanol and heating for a few minutes at 105 °C. Agricultural , qualitative identification 14

61 099 C.L. CURL*, K.R. PRICE, G.R. FENWICK, (*AFRS Inst. of Food Research, Norwich Laboratory, Colney Lane, NorwichNR 4 74A, UK): Soyasaponin A3, a new monodesmosidic saponin isolated from the seeds of Glycine max. Journal of Natural Products 51, 122-124 (1988). TLC of 3-O-(a-L-rhamno-pyranosyl(1,2)-ß-D-galactopyranosyl(1,2)-ß-D-glucuronopyranosyl)olean-12-en- 21ß, 22ß-24-triol on silica(normal phase) with n-butanol - ethanol - NH3 7:2:5, and on silica, impregnated with oxalic acid, with chloroform -methanol - water 65:35:10 lower phase, and on RP-18 silica with methanol - water 3:2. Agricultural , qualitative identification 14

61 100 N. FISCHER, S. NITZ, F. DRAWERT, (Inst. f. Lebensmitteltechnologie u. Anal. Chemie, Techn. Univ. München, D-8050Freising-Weihenstephan, F.R.G.): Über gebundene Aromastoffe in Pflanzen - 2. Mittlg.: Freie und gebundeneAromastoffe in grünem und schwarzem Tee. (Bound flavour compounds in plants - 2. Part. Free and bounded flavourcompounds in green and black tea.) Z. Lebensm. Unters. Forsch. 185, 195-201 (1987). TLC of n-amyl-, n-decyl andphenyl-ß-D-glucopyranosides on HPTLC silica with chloroform - methanol - water 16:9:2. Detection under UV 254 nmand spraying with 2% diphenylamine in acetone/2% aniline in acetone/85% H3PO4 5:5:2.

14

61 101 A. HERMANS-LOKKERBOL, R. VERPOORTE, (Center f. Bio-Pharmaceut. Sci., Dep. of Pharmacog., Leiden Univ.,P.O. Box 9502, NL-2300 RA Leiden): Purification of secologanin from Lonicera tatarica extracts using RLCC. Planta Med. 53, 546-548 (1987). TLC on silica with the organic layer of ternary or quaternary solvent systems. Themethod is applied for the selection of eluents for rotation locular counter-current chromatography (RLCC) and for theanalysis of the separated fractions. Suitable solvent systems are given: chloroform - methanol - n-propanol - water5:12:1:8; 9:12:1:8; chloroform - methanol - water 43:37:20; ethyl acetate - n-propanol - water 4:2:7; n-butanol -n-propanol - water 2:1:3. Qualitative identification , natural products isolation 14, 4

61 102 R.L. LINDROTH*, M.T.S. HSIA, J.M. SCHRIBER, (*Dep. of Entomology, Univ. of Wisconsin, 1630 Linden Drive,Madison, WI. 53706, USA): Characterization of phenolic glycosides from quaking aspen. BiochemicalSystematics and Ecology 15, 677-680 (1987). TLC of the phenolic glycosides salicin, salicortin, tremuloidin andtremulacin on silica with dichloromethane - methanol 4:1. Visualization under UV at 254 nm. Agricultural , qualitative identification 14

61 103 P. XIE (Xie Peishan), Y. YAN (Yan Yuzhen), (Guangzhou Inst. for Drug Control, 23 Xizeng Rd., Xicun,Guangzhou/Guangdong, P.R. China): HPTLC fingerprint identification of commercial Ginseng drugs -reinvestigation of HPTLC of ginsenoides. J. High Resol. Chromatogr. 10, 607-613 (1987). TLC of ginsenoside-Rx(Rb1, Rb2, Rb3, Rc, Rd, Re, Rf, Rg1, -Rg2, F2, pseudoginsenoside-F11, notoginsenoside-R1) on HPTLC silica plateswith chloroform - ethyl acetate - methanol - water 15:40:22:10 (lower phase after standing overnight at 8-10°C).Detection after dipping into 5% sulfuric acid - ethanol solution for 2 sec., heating at 105 °C for 60 sec. and then, aftercooling dipping into liquid paraffin/hexane 1:1 for 2 sec. to stabilize the fluorescence intensity. Quantification byfluorescence scanning at 366/>400 nm. Quantitative analysis , densitometry , ginsenosides 14



15. Terpenes and other volatile plant ingredients

61 104 S.A. ELMARAKBY*, F.S. EL-FERALY, H.A. ELSOHLY, E.M. CROOM, C.D. HUFFORD, (*Dep. of Pharmacognosy andResearch Inst. of Pharmaceutical Sciences, Univ. of Mississippi, Mississippi 38677, USA): Microbiologicaltransformation studies on arteannuin B. J. of Natural Products 50, 903-909 (1987). TLC study of the microbialtransformation of the sesquiterpene lactone arteanuuin B. Chromatography on silica with ether - hexane 4:1.Visualization with anisaldehyde-sulfuric acid spray reagent. Pharmaceutical research , qualitative identification 15a

61 105 C.A.J. ERDELMEIER, P.A.S. VAN LEEUWEN, A.D. KINGHORN, (Dep. of Med. Chem. and Pharmacog., Univ. ofIllinois at Chicago, Chicago, Illinois 60612, USA): Phorbol diester constituents of croton oil: separation bytwo-dimensional TLC and rapid purification utilizing reversed- phase overpressure layer chromatography(RP-OPLC). Planta Med. 54, 71-75 (1988). Separation of phorbol esters by two-dimensional TLC on dual-phaseplates: 1. normal phase, chloroform - acetone 4:1 and 2. reversed-phase, methanol - water 9:1. Detection withethanolic sulfuric acid (60%) at 110°C. Also overpressure layer chromatography on RP-18 with methanol - water 9:1.Flow-rate 0.1 mL/min. Toxicology , qualitative identification , comparison of methods 15

61 106 S.G. GARG*, S.K. AGARWAL, V.P. MAHAJAN, S.N. RASTOGI, (*Central Inst. of Medicinal and Aromatic Plants,Lucknow-226016, India): Feruginidin and ferugin, two new sesquiterpenoids based on the carotene skeletonfrom Ferula jaeschkeana. Journal of Natural Products 50, 253- 255 (1987). TLC on silica with chloroform - methanol85:15. Detection by spraying with vanillin-sulfuric acid reagent and heating for 10 min. at 100 °C. An acidic hydrolysisfraction of feruginidin and ferugin was identified as p-hydroxybenzoic acid. Agricultural , qualitative identification 15a

61 107 A. GOSWAMI*, J.M. BEALE, R.L. CHAPMAN, D.W. MILLER, J.P. ROSAZZA, (*Div. of Medicinal Chemistry and NaturalProducts, Coll. of Pharmacy, Univ. of Iowa, Iowa City, Iowa 52242, USA): Microbial hydroxylation of quadrone to8a-hydroxy-quadrone. Journal of Natural Products 50, 49-54 (1987). TLC of quadrone, quadrone-4-ethylene ketal,8a-hydroxyquadrone, 8a-hydroxyquadrone-4-ethylene ketal on silica with chloroform - acetone - benzene - formic acid95:5:5:1 and chloroform - ethyl acetate 3:1. Visualization by spraying with p-anisaldehyde - acetic acid - sulfuric acid1:120:1 and heating. Pharmaceutical research , qualitative identification 15a

61 108 K. HILLER*, G. BADER, H.R. SCHULTEN, (*Sektion Chemie der Humboldt-Univ. zu Berlin, DDR-1120 Berlin,Goethestr. 54): Virgaureasaponin 1, ein neues bisdesmosidisches Triterpensaponin aus Solidago virgaurea L. Virgaureasaponin 1, a new bisdesmosidic triterpenoid saponin of Solidago virgaurea L. Pharmazie 42, 541-543 (1987).TLC of saponins on silica with chloroform - methanol - water 15:10:2, with chloroform - methanol 9:1 and with n-butanol- ethanol - water 4:1:5 (upper phase). Detection reagents: blood gelatin for saponins, anisaldehyde - sulfuric acid forsaponins and sapogenins and thymol - sulfuric acid for sugars. A new triterpenesaponin isolated and identified. Agricultural , qualitative identification 15a

61 109 A. KERY, G. PETRI*, (*Semmelweis Medical Univ., Inst. of Pharmacognosy, H-1085 Budapest, Üllöi u.26): Development and application of complex screening method for the study of biologically active sesquiterpenelactones. Herba Hungarica 26, 159-170 (1987). TLC of sesquiterpene lactone on silica with benzene - n-hexane -acetonitrile 30:20:21 and carbon tetrachloride - acetonitrile 40:1.5. Detection by spraying with a) 2% sulfuric acid inethanol, heating at 110°C for 2-3 min, and b) phosphomolybdic acid and heating at 100°C for 2 min. OPTLC on silicawith 1. carbon tetrachloride - acetonitrile 4:1.5, 2. n-hexane - ethyl acetate 2:1, 3. n-hexane - ethyl acetate 7:3, 4.n-hexane - acetonitrile - benzene 20:21:30. Densitometry by absorbance. Pharmaceutical research , densitometry , qualitative identification 15a

61 110 A. KERY, GY. TURIAN*, I. ZSAMBO, P. TETENYI, (*Research Inst. for Medicinal Plants, Budakalász, Hungary): Comparison of different separation techniques on the biologically active sesquiterpene lactones. Acta Pharm.Hungarica 57, 228-238 (1987). TLC and OPTLC of artemorin, partenolide, tatridin, costunolide, cnicin, santamarin,umbellifolide, taurin, reynosin (sesquiterpene lactone compounds). TLC on silica with benzene - acetonitrile - n-hexane30:21:20 or carbon tetrachloride - acetonitrile 40:15. Detection by spraying with 1. 2% sulfuric acid in ethanol, thenheating at 110 °C for 2-3 min.; 2. phosphomolybdic acid reagent, then heating at 100 °C for 2 min. Densitometry byabsorbance at 520 and 600 nm. OPTLC on silica with carbon tetrachloride - acetonitrile 4:1.5, n-hexane - ethyl acetate2:1 or 7:3, benzene - acetonitrile - n-hexane 3:2.1:2. Detection and evaluation methods same as in TLC. Pharmaceutical research , densitometry 15a

61 111 S.F. KIMBU*, B. NGADJUI, L.B. SONDENGAW, (*Dep. of Organic Chemistry, Univ. of Yaounde, Cameroon): A new



61 111 S.F. KIMBU*, B. NGADJUI, L.B. SONDENGAW, (*Dep. of Organic Chemistry, Univ. of Yaounde, Cameroon): A newlabdane diterpenoid from the seeds of Aframomum daniellii. Journal of Natural Products 50, 230-231 (1987). TLCon silica with 1% ethyl acetate in hexane. Agricultural , qualitative identification 15a

61 112 X. LIU (Liu Xunhong), C. Song (Song Caicheng), (Nanjing Coll. Trad. Chin. Med., Nanjing, P.R. China): (Identificationof argy wormwood, Artemisia argri, and lavenderleaf wormwood, A. lavandulae folia). (Chinese). J. Chinese HerbMed. 19, 83-84 (1988) (Zhongcaoyao). TLC of essential oil ingredients on silica with chloroform. Detection by sprayingwith 1% vanillin in sulfuric acid. Pharmaceutical research , qualitative identification , essential oil 15b, 32e

61 113 L.J. LOCKEN*, R.G. KELSEY, (*Chemistry Departm., Univ. of Montana, Missoula, MT 59812, USA): Cnicinconcentrations in Centaurea maculosa, spotted knapweed. Biochemical Systematics and Ecology 15, 313-320(1987). TLC on silica with chloroform - petrol ether - ethanol 5:4:1. Visualization by spraying with conc. sulfuric acid andcharring overnight at 100°C. Agricultural , qualitative identification 15a

61 114 R.CH. RACHEV, S.V. BOJKOVA, R. PAVLOVA-ROUSEVA, V.K. GANCHEVA, M.P. DIMITROVA, (Inst. ofMicrobiology, Bulgarian Acad. of Sciences, 1113 Sofia, Bulgaria): Separation of gibberellins by thin-layerchromatography and gas chromatography, and possibilities for their quantitative analysis. J. Chromatogr. 437,287293 (1988). TLC of gibberellins methylated with diazomethane in a culture filtrate of Fusarium moniliforme on silicawith ethyl acetate - chloroform - methanol - acetic acid 20:20:2:0.2. Detection with 50% sulfuric acid in ethanol.Densitometry by fluorescence. Method suitable for monitoring of fermentation processes. Agricultural , quantitative analysis , densitometry 15

61 115 Y. ROISIN, J.M. PASTEELS*, J.C. BRAEKMAN, (*Collectif de Bio-écologie, CP 160, Univ. Libre de Bruxelles, 50 av.F.D.Roosevelt, B-1050 Bruxelles, Belgium): Soldier diterpene patterns in relation with aggressive behaviour,spatial distribution and reproduction of colonies in Nasutitermes princeps. Biochemical Systematics andEcology 15, 253-261 (1987). TLC of 22 different diterpenes (from 11 unidentified compounds) on silica with hexane -ethyl acetate 7:3. Agricultural , qualitative identification 15a

61 116 J.E. THOMPSON*, P.T. MURPHY, P.P. BERGQUIST, E.A. EVANS, (*Australian Inst. of Marine Science, P.M.B. No. 3,Townsville M.C., Queensland, 4810, Australia): Environmentally induced variation in diterpene composition of themarine sponge Rhopaloeides odorabile. Biochemical Systematics and Ecology 15, 595-606 (1987).Two-dimensional TLC of 3a,19-dihydroxyspongia-13(16),14-dien-2-one,3a,17,19-trihydroxyspongia-13(16),14-dien-2-one on silica with dichloromethane - ethyl acetate 4:1 (first direction) and1:1 (second direction). Detection by spraying with vanillin-sulfuric acid reagent and heating. Environmental , qualitative identification 15a

18. Amino acids and peptides, chemical structure of proteins

61 117 W. BAEYENS*, G. VAN DER WEKEN, P. DE MOERLOOSE, (*State Univ. Ghent, Lab. Pharm. Chem. and Drug Anal.,Pharm. Inst., Harelbekestraat 72, B-9000 Ghent, Belgium): Rapid thin-layer chromatography of homocysteine -cysteine mixtures as their thiolyte-derivatives. Chromatographia 23, 289-291 (1987). TLC of fluorescent-labelledcysteine and its homologue homocysteine with monobromo-bimane on silica with 4 different solvent systems.Identification by their intense yellow fluorescence under UV at 366 nm. Detection limit 2 ng for both. Qualitative identification , cysteine , homocysteine , thiolyte-derivatives 18a

61 118 SH. DANG (Dang Shuqin), Q. LI (Li Qingshu), (Isotope Dep., Inst. Atom.Energy, Beijing, P.R. China): (Study of thepaper and thin-layer chromatographic behaviours of 75Se-methionine). (Chinese). Chinese J. Chromatogr. 6,46-48 (1988) (Sepu). Investigation of TLC behaviours of 75Se-methionine sulfoxide, 75Se-methionine sulfone andselenoxide on silica with ethanol - water in various proportions. Detection by spraying with ninhydrin reagent. 75Se-methionine 18a

61 119 J. ERCHEGYI*, J. SEPRÖDI, K. NIKOLICS, I. TEPLAN, ZS. VADASZ, M. KOVACS, B. KANYICSKA,(*Chemical-Biochem. Inst., Pécs, Hungary): Synthesis and biological activity of superactive GnRH-analoguescontaining in position six D-amino-acids carrying strongly lyophilic side-chains. (Hungarian). KémiaiKözlemények 65, 9-19 (1986). TLC of (D-thyroxine6)-GnRH, (D-thyronine6)-GnRH,(D-4-chloro-D-phenylalanine6)-GnRH, (D-thyroxine6)-GnRH-(1-9)-ethylamide, (D-thyronine6)-GnRH-(1-9)-ethyl-amide,(D-4-chloro-D-phenylalanine6)-GnRH-(1-9)-ethylamide on silica with 11 different solvent systems. Pharmaceutical research , qualitative identification 18a



61 120 K. GUENTHER*, R. RAUSCH**, (*Degussa AG, Forschung Chemie Organisch, D-6450 Hanau, FRG,**Macherey-Nagel, P.O. Box 307, D-5160 Düren, FRG): Quantitative determination of TLC- separated enantiomersof amino acids. H. TRAITLER, A. STUDER, R.E. KAISER (eds): Instrumental HPTLC, Institute for Chromatography,Bad Dürkheim, FRG (1987), 155-165. TLC on chiral plate with methanol - water - acetonitrile 50:50:200, 50:50:30 and50:70:200. Detection by dipping in a solution of 0.3 % ninhydrin in acetone and heating at 110 °C for 15 min.Quantification by densitometry. Detection limits, 0.1 % of the minor enantiomer. Quantitative analysis , densitometry , enantiomers , amino acids 18a, 3b

61 121 P. HENKLEIN*, M. BOOMGAARDEN, E.M. NIEKE, M. GEORGI, H. NIEDRICH, (*Inst.für Wirkstofforschung derAkademie der Wissenschaften der DDR, Alfred-Kowalke-Str.4, DDR-1136 Berlin): Eine verbesserte Synthese vonPentagastrin (Acignost). An improved synthesis of pentagastrin (Acignost). Pharmazie 43, 10-12 (1988). TLC of thepentapeptide boc-ß-ala-trp-met-asp- phe on silica with chloroform - methanol - acetic acid 18:2:1, sec. butanol - 3%NH3 25:11 and 75:25, ethyl acetate - acetic acid - water 20:6:11, ether - acetic acid - water 77:15.4:7.6 and n-butanol -acetic acid - water 40:10:50 (upper phase). Detection of the peptide derivatives with ninhydrin-, chlor/TDM-, Ehrlich’s-and fluorescamine reagent. Pharmaceutical research , qualitative identification 18b

61 122 T.D. KIMBROUGH*, J.D. REYNOLDS, K.J. HUMPHREYS, L.B. WEEKLEY, (*Depart. of Biology, VirginiaCommonwealth Univ. Richmond, USA): Diurnal changes in tissue leaf levels of tryptophane, tyrosine and aminemetabolites in Sedum morganianum and Sedum pachyphyllum. Biochemie und Physiologie der Pflanzen 182,67-72 (1987). TLC of tryptophane, tyrosine, norepinephrine, serotonine and 5-hydroxyindole-3-acetic acid on silica withbutanol - acetic acid - water 4:1:5. Running time 2 h at 25°C. After drying, spraying with OPT (indoles) or with1-nitrose-2-naphthol. After 12 h examination under UV. Agricultural , qualitative identification 18a, 17b

61 123 K. KUMARI, R.P. SINGH, S.K. SAXENA, (Section of Plant Path. and Nematology, Dept. Botany, Aligarh MuslimUniv.Aligarh 202001, India): Effect of different factors on the movement of some amino acids in soils usingthin-layer chromatography. J. Liquid Chrom. 10, 1299-1325 (1987). Study of the influence of e.g. organic matter,saline and alkaline salts, cations and pH on the mobility of mono-, dicarboxylic, basic and aromatic amino acids usingsoil thin-layer chromatography. Discussion of mobility in terms of Rf values of amino acids under different treatments. Agricultural 18a, 3b

61 124 S. LASKAR, B. BASAK, (Natural Prod. Lab., Chem. Dep., Burdwan Univ., Burdwan-713 104, West Bengal, India): Detection of amino acids on thin-layer plates. J. Chromatogr. 436, 341-343 (1988). TLC of various amino acids onsilica with methanol - chloroform - 3:1. Detection by spraying the plates first with 0.01% fluorescein isothiocyanate in0.1 M aq. sodium hydroxide solution. After heating at 90 °C for 10 min., the plates are cooled and then sprayed with0.25 % ninhydrin in acetone. Various different colors are obtained. Detection limit 0.5-1.0 µg. Pharmaceutical research , qualitative identification 18a

61 125 P. RAKOWSKI*, P. MELIN, B. LAMMEK, (*Inst. of Chemistry, Univ. of Gdansk, Sobieskiego 18, 80-952 Gdansk,Poland): Synthesis and some pharmacological properties of two new antagonists of oxytocin. Polish Journal ofPharmacology and Pharmacy 39, 303-307 (1987). TLC of two new 1-ß-ß-disubstituted analogues of oxytocinpolypeptide hormones on silica with chloroform - methanol 7:3 or 1-butanol - acetic acid - water - pyridine 15:3:3:10 or1-butanol - acetic acid - water 4:1:5 or ethanol - 0.1 mol/L aqueous pyridine - 0.1 mol/L aqueous acetic acid 4:1:1 orbenzene - methanol - acetic acid 2:15:1 or n-hexane - ethyl ether - acetic acid 11:7:1 or benzene - methanol - aceticacid 15:2:1. Visualization with ninhydrin or iodine. Pharmaceutical research , qualitative identification 18b

61 126 J. REN (Ren Jianming), T. ZHAO (Zhao Tianrui), G. NAN (Nan Guohua), (Dep. Biochem., Jiangxi Med. Coll.,Nanchang, Jiangxi, P.R. China): (Determination of some amino acids in cerebrum of rat by thin-layerchromatography). (Chinese). Chinese J. Adv.in Biochem.& Biophys. 2, 37-40 (1987) (Shengwu Huaxue Yu ShengwuWuli Jinzhan). TLC of 6 amino acids on polyamide with a) benzene - acetic acid 9:1, b) 1.3% aqueous formic acid, c)ethyl acetate - methanol - acetic acid 20:1:1. Detection by dansylation. Quantification by fluorometry. Clinical chemistry research , quantitative analysis , densitometry , amino acids 18a

61 127 K.J. RUTEBORIES, D. NUROK*, (*Dep.Chem., Indiana Univ.-Purdne Univ.at Indianapolis, Indianapolis, Indiana 46223,USA): Thin-layer chromatographic separation of diastereomeric amino acid derivatives prepared with Marfey’sreagent. Anal. Chem. 59, 2735-2736 (1987). TLC of the diastereomeric 1-fluoro-2,4-dinitro-phenyl-5-L-alanine amidederivatives of 22 amino acids on RP-18 silica with methanol - sodium acetate, and on silica with acetic acid - tert.butylmethyl ether in various proportions. Detection by fluorescence quenching. Comparison of the results of the twoprocedures. Clinical chemistry research , diastereomeric amino acid derivatives , amino acids 18a



61 128 G.K. TOTH*, B. PENKE, J. NAGY, B. SZAJANI, J. ZSIGO, M. ZARANDI, K. KOVACS, (*Inst. of Medical Chemistry,Univ. Med. School, H-6720 Szeged, Dóm tér 8., Hungary): Synthesis and HPTLC purification of the renin substratetetradecapeptide. Acta Chimica 124, 595-601 (1987). HPTLC purification of the peptide on silica with ethyl acetate -n-butanol - acetic acid - water 1:1:1:1 or n-butanol - acetic acid - water 8:5:4. Detection by spraying with ninhydrin andchlorine-tetramethyl-diaminodiphenylmethane. Pharmaceutical research , qualitative identification 18b

22. Alkaloids

61 129 M.L. ABOUCHACRA*, M. LEBOENF, H. GUINANDEAN, A. CAVÉ, (*Lab. de Pharmacognosie, Faculté de Pharmacie,UA 496 CNRS, 92296 Chatenay-Malabry Cedex, France): The bisbenzylisoquinoline alkaloids of Pycnarrhenaozantha. Journal of Natural Products 50, 375-380 (1987). TLC of (+)-2-northalrugosine, (+)-bisnorobamegine,(+)-bisnorthalrugosine and (+)-pycnazanthine on silica with acetonitrile - benzene - ethyl acetate - NH3 40:30:20:10 andone other system. Detection with Dragendorff’s reagent or with FeCl3 (1 mL 0.5M) in perchloric acid (35%, 50 mL) andheating. Pharmaceutical research , qualitative identification 22

61 130 I.A. AL-MESHAL*, K.A. AL-RASHOOD, M.NASIR, F.S. EL-FERALY, (*King Sand Univ., Coll. of Pharmacy, Riyadh11451, Saudi Arabia): (-)-cathinone: improved synthesis and carbon-13 NMR assiguments. Journal of NaturalProducts 50, 1138-1140 (1987). TLC of (-)- and (+)-cathine(nor-pseudoephedrine) on silica with chloroform - acetonitrile7:3. Detection under UV. Pharmaceutical research , qualitative identification 22

61 131 M. FERRARI, L. VEROTTA, (Dipart. Chim. Organica e Industr., Univ. degli Studi di Milano, Via G. Venezian 21, I-20133Milan): Centrifugal thin-layer chromatography of alkaloids from vegetable sources using an aluminium oxidelayer. J. Chromatogr. 437, 328-332 (1988). Preparative separation of closely related alkaloids from Papaver andFumaria species on aluminium oxide by centrifugal TLC with cyclohexane - toluene - diethylamine 320:80:1, 160:40:1or 160:40:3. Flow-rate 0.5-1.5 mL/min. The rotors (1 mm layer thickness) were prepared from a mixture of 40 g basicaluminium oxide, 10g calcium sulfate hemihydrate and 110 mL water. Pharmaceutical research , preparative TLC 22, 4b

61 132 A.J. FREYER*, M.H. ABU ZARGA, S. FIRDONS, H. GUINANDEAN, M. SHAMMA, (*Dep. of Chemistry, ThePennsylvania State Univ., Univ. Park, Pennsylvania 16802, USA): Five new alkaloids from Colchicum ritchii. Journal of Natural Products 50, 684-689 (1987). TLC of (-)autumnaline, (-)isoautumnaline, (-)specioritchine,(-)speciocolchine, (+)androcymbine and (+)colchiritchine on silica with chloroform - acetone - diethylamine 80:10:10,ethyl acetate - ethanol 75:25 and benzene - chloroform - diethylamine 80:10:10. Agricultural , qualitative identification 22

61 133 V. GRASSERT*, H. LELLBACH, (*Inst. f. Kartoffelforschung der Akademie der LWS-Wissenschaften der DDR, GrossLüsewitz, Kreis Rostock, Germany-DDR-2551): Untersuchung des Glykoalkaloidgehaltes von Kartoffelhybridenmit Resistenz gegen die Kartoffelnematoden Globodera rostochiensis und Globodera pallida. (Study of theglycoalkaloid content in potato hybrids with resistance to potato nematodes Globodera rostochiensis and GloboderaPallida). Biochemie und Physiologie der Pflanzen 182, 473-479 (1987). TLC of glycoalkaloids on silica with chloroform- methanol - 1% NH3 2:2:1 (lower phase). Detection by spraying with antimony trichloride and heating for 10 min. at85 °C. Food analysis , quantitative analysis , qualitative identification 22

61 134 N. GRESPI-PERELLINO*,M. BALLABIO,B. GIOIA,A. MINGHETTI,(*Farmitalia Carlo Erba, 20146 Milano, via deiGracchi 35, Italy): Two unusual ergopeptines produced by a saprophytic culture of Claviceps purpurea. Journalof Natural Products 50, 1065-1074 (1987). TLC of the main ergotamine and ergotoxine group alkaloids on silica withdichloromethane - isopropanol 92:8 or dichloromethane - methanol 90:10. Detection under UV at 254 nm and 366 nmand by spraying with N,N-p-dimethylaminobenzaldehyde. Agricultural , qualitative identification 22

61 135 CH. LIU (Liu Chengji), X. ZENG (Zeng Xuan), G. LIN (Lin Ge), D. TANG (Tang Diguang), J. MOU (Mou Jianquan),(Nanjing Coll. Pharm., Nanjing, P.R. China): (Separation and determination of the poisonous alkaloids in AconitumKusnezoffii and its processed products) (Chinese). J. Chinese Trad. Med. 12, 83-86 (1987) (Zhongyao Tongbao).TLC of aconitine, mesaconitine and hypaconitine in A. Kusnezoffii root on silica with ether - chloroform 2.5:1 saturatedwith 25% NH3. Determination by densitometry at 240 nm. Pharmaceutical research , quantitative analysis , densitometry , alkaloids 22, 32e

61 136 M.F. ROBERTS*, T.M. KUTCHAN, R.T. BROWN, C.J. COSCIA, (*Dep. of Pharmacognosy, London Univ., London



61 136 M.F. ROBERTS*, T.M. KUTCHAN, R.T. BROWN, C.J. COSCIA, (*Dep. of Pharmacognosy, London Univ., LondonWC1N 1AX, Great Britain): Implication of tyramine in the biosynthesis of morphinan alkaloids in Papaver. Planta 172, 230-237 (1987). After extraction of morphine, codeine, papaverine, thebaine and reticuline from Papaver,TLC on silica with acetone - toluene - ethanol - NH3 40:40:12:4. HPTLC of morphine and codeine with chloroform -n-butanol - methanol NH3 40:30:30:10 and of thebaine, reticuline and papaverine with toluene - ethanol 80:20.Quantification, after elution, with HPLC. Agricultural , qualitative identification , preparative TLC 22

61 137 W. RUDOLF, T. KNACKER*, H. SCHAUB, (*Friedrichsdorfer Strasse 35, D-6000 Frankfurt 56, FRG): The effect oflow oxygen concentration on the cytokinin content of the C4 plant Amaranthus paniculatus L. Biochemie undPhysiologie der Pflanzen 182, 203-211 (1987). TLC of cytokinin[9-beta-D-ribofuranosyl-6-(3-methylbut-2-enylamino)purine] on silica with chloroform - methanol 90:10. Detection underUV 254 nm. Agricultural , qualitative identification 22

61 138 CS. SIMON*, S. BERENYI, S. MAKLEIT, V. FEKETE, (*Alkaloida Chemical Factory, Tiszavasvári, Hungary): Conversions of tosyl and mesyl derivatives of the morphine group, XXV. , Studies of the nucleophilic substitutionreactions of 6-O-mesyl-7a-chloro(bromo)neopine. Acta Chimica 124, 497-503 (1987). TLC of 7a-chloro-neopine and7a-bromo-neopine on silica with benzene - methanol 8:2 or chloroform - methanol 9:1. Detection by spraying withDragendorff-reagent. Pharmaceutical research , qualitative identification 22

61 139 A. URZUA*, A.J. FREYER, M. SHAMMA, (*Dep. of Chemistry, The Pennsylvania State Univ., Univ. Park, Pennsylvania16802): Aristolodione, a 4,5-dioxoaporphine from Aristolochia chilensis. Journal of Natural Products 50, 305-306(1987). TLC of aristolodione on silica with chloroform - methanol 95:5 and benzene - ethyl acetate 3:1. Detection byspraying with Dragendorff’s reagent. Agricultural , qualitative identification 22

61 140 G. WASSEL*, B. EL-MENSHAWI, A. SAEED, G. MAHRAN, M. EL-MERZABANI, (*Pharmaceutical SciencesLaboratory, National Res. Centre, Dokki-Cairo, A.R.E.): Screening of selected plants for pyrrolizidine alkaloidsand antitumor activity. Pharmazie 42, 709-710 (1987). TLC on silica with chloroform - methanol 9:1, benzene -methanol 9:1, chloroform - methanol - NH3 8:1:1. Detection with Dragendorff’s and Mattock’s reagent (see J.Chromatogr. 27, 505, 1967). Agricultural , qualitative identification 22

61 141 W. ZENG (Zeng Weiguang), W. LIANG (Liang Wenzao), G. TU (Tu Guoshi), (National Inst. Cont. Pharm. & Biol. Prod.,Beijing, P.R. China): (Quantitative determination of tertiary alkaloids in Corydalis bungeana Turcz by thin-layerchromatography) (Chinese). Chinese J. Pharm. Anal. 7, 323-327 (1987) (Yaowu Fenxi Zazhi). TLC ofacetylcorynoline, 13-epicorynoline, corynoline, 11-epicorynoline, cheilanthifoline, corycavine and protopine on silicawith dichloromethane - benzene - ether 5:4:0.5 for acetylcorynoline, and 7:5:2.5 for others. Detection by spraying withDragendorff’s reagent. Quantification by densitometry at 500 nm. Pharmaceutical research , quantitative analysis , densitometry , tertiary alkaloids 22, 32e

61 142 X. ZHANG (Zhang Xicun), X. TIAN (Tian Xiuli), (Shenyang Coll. Pharm., Shenyang, P.R. China): (Quantitativedetermination of berberine in Gold thread, Coptis chinesis, by thin-layer chromatography) (Chinese). J.Chinese Herb Med. 18, 394-396 (1987) (Zhong Caoyao). TLC on silica with the upper phase of butanol - 36% aceticacid - water 2:1:1. Quantification by fluorodensitometry at 334 nm. Pharmaceutical research , quantitative analysis , densitometry , berberine 22, 32e

23. Other substances containing heterocyclic nitrogen

61 143 M.E. WALL*, H. TAYLOR, P. PERERA, M.C. WANI, (*Res. Triangle Inst., Res. Triangle Park, North Carolina 27709,USA): Indoles in edible members of the cruciferae. Journal of Natural Products 51, 129-135 (1988). TLC ofindole-3-carboxaldehyde and indole-3-acetonitrile on silica with dichloromethane and with dichloromethane - methanol95:5 and 90:10. Color reaction after spraying with Ehrlich’s reagent. Pharmaceutical research , agricultural , qualitative identification 23c

61 144 T.M. WOLF*, R. WASSERSTEIN, J.S. YOON, (*Departm. of Biology, Washburn Univ. of Topeka, KS, USA):



61 144 T.M. WOLF*, R. WASSERSTEIN, J.S. YOON, (*Departm. of Biology, Washburn Univ. of Topeka, KS, USA): Qualitative and quantitative studies of pteridine eye pigments in Drosophila. Seven species of HawaiianDrosophila and D. melanogaster. Biochemical Systematic and Ecology 15, 239-245 (1987). Two-dimensional TLC ofpteridines, biopterin, 2-amino-4- hydroxypterin on cellulose with 6% acetic acid and 3% ammonium chloride.Quantification by fluorometry. Pharmaceutical research , quantitative analysis , qualitative identification 23

61 145 G. ZHANG (Zhang Guangzheng), G. LI (Li Guangping), ZH. LI (Li Zhu), (Shanghai Inst. Planned Parenthood Res.,Shanghai, P.R. China): (Determination of acriflavine by thin-layer chromatography) (Chinese). Chinese J. Med.Industry 17, 422-423 (1986) (Yiyao Gongye). TLC on silica with butanol - isopropanol - 38% NH3 3:1:0.5.Quantification by densitometry at 460 nm. Pharmaceutical research , quantitative analysis , densitometry , acriflavines 23e

24. Organic sulfur compounds

61 146 A. ZEKANY*, S. MAKLEIT, (*Biogal Pharmaceutical Works, H-4010 Debrecen, Pallagi u. 13, Hungary.): Synthesis of7ß-[4-oxo-3,4-dihydrothienol(2,3-d)pyrimidyl-2-thioacetamido]cephalosporanic acid derivatives. Pharmazie 42,160-161 (1987). TLC of thieno (2,3-d) pyrimidine on silica with chloroform - methanol - acetic acid 9:2:1. Visualizationunder UV. Pharmaceutical research , qualitative identification 24

27. Vitamins and various growth regulators

61 147 S. EBEL, M. HERBOTH, (Univ. Würzburg, Dep. Pharm., Am Hubland, D-8700 Würzburg, FRG): Stability evaluationof thiamine (vitamin B1) by HPTLC. H. TRAITLER, A. STUDER, R.E. KAISER (eds): Instrumental HPTLC, Institutefor Chromatography, Bad Dürkheim, FRG (1987), 121-131. HPTLC on silica with methanol - butanol - 7% NaCl(H2O)45:85:50. Determination by densitometry at 270 nm. Discussion of some aspects of HPTLC having negative influencefor long range calibration procedures. Pharmaceutical research , quantitative analysis , densitometry , thiamine 27

61 148 J. TAN (Tan Jianzhen), Y. Yan (Yan Yuzheng), (Guangzhou Jingxiutang Pharm. Plant, Guangzhou, P.R. China): (Quantitative analysis of olaquindox in feed „Fei Zhu Su“ by thin-layer chromatography) (Chinese). ChineseAnal. Chem. 15, 939-941 (1987) (Fenxi Huaxue). TLC on silica with benzene - acetic acid - ethanol - water 70:25:10:3.Detection under UV 365 nm. Quantification by densitometry at 255 nm. Agricultural , quantitative analysis , densitometry , olaquindox 27

28. Antibiotics, Mycotoxins

61 149 J. ALLWOHN, S. EBEL, J.S. KANG, (Univ. Würzburg, Dep. Pharm., Am Hubland, D-8700 Würzburg, FRG): Analysisof aminoglycosid antibiotics by HPTLC and electrophoretic procedures. H. TRAITLER, A. STUDER, R.E.KAISER (eds): Instrumental HPTLC, Institute for Chromatography, Bad Dürkheim, FRG (1987), 27-33. TLC on silicawith methanol - 25% NH3 3:2. Detection by dipping into ninhydrin reagent. Quantification by densitometry at 590 nm.Detection limits, 20 ng for neomycin and 8 ng for netilmycin. Also, electrophoretic method. Pharmaceutical research , quantitative analysis , densitometry , aminoglycosid antibiotics 28a

61 150 H. ASUKABE, H. YONEYAMA, Y. MORI, K. HARADA, M. SUZUKI, H. OKA, (Fac. Pharm., Meijo Univ., Nagoya, Japan468): Improvement of chemical analysis of antibiotics. XI. Simultaneous fluorodensitometric determination ofpolyether antibiotics. J. Chromatogr. 396, 261-271 (1987). J. Chromatogr. 396, 261-271 (1987). TLC ofsalinomycin,monensin and lasalocid on silica and RP-18 silica with carbon tetrachloride - ethyl acetate - acetonitrile50:5:10 or ethyl acetate - acetone - acetonitrile 15:1:55. Fluorescence derivatization by reaction of sodium salts with1-bromoacetylpyrene and Kryptofix 222 in acetonitrile. Determination by densitometry using 18,19-dihydrosalinomycinand 18,19-dihydro-20-ketosalinomycin as internal standards. Detection limit 100 pg. Pharmaceutical research , quantitative analysis , densitometry , polyether antibiotics 28a

61 151 D.G. CORLEY*, G.E. ROTTINGHAUS, M.S. TEMPESTA, (*Department of Chemistry, Univ. of Missoure, Columbia,Missouri 65211, USA): Secondary metabolites from Fusarium. Two new modified trichothecenes from Fusariumsporotrichioides MC-72083. Journal of Natural Products 50, 897-902 (1987). TLC of 8a- and 8ß-hydroxysambucoin onsilica with benzene - acetone 3:2. Vizualization with two reagent systems: 1) 0.5% p-anisaldehyde in methanol - aceticacid - sulfuric acid 85:15:5 and 2) 1% 4-(p-nitrobenzyl)-pyridine in carbon tetrachloride - chloroform 3:2 followed, afterheating for 30 min. at 150 °C., with 10% tetraethylenepentamine in carbon tetrachloride - chloroform 3:2. Theepoxide-containing trichothecenes give a skyblue color.



Toxicology , qualitative identification 28b

61 152 O.J. FRANCIS, G.M. WARE, A.S. CARMAN, G.P. KIRSCHENHEUTER, S.S. KUAN, R.F. NEWELL, (Food and DrugAdmin., Natural Toxins Res. Center, 4298 Elysian Fields Ave., Ney Orleans, LA 70122, USA): Thin-layerchromatographic determination of sterigmatocystine in cheese: interlaboratory study. J.A.O.A.C. 70, 842-844(1987). TLC on HPTLC silica with hexane - methanol 95:5. Quantification after treatment of the plate withfluorescence-enhancing spray consisting of 15% AlCl3 in ethanol and silicane - ether 18:82. Method acc. to O.J.Francis et al., J.A.O.A.C. 68, 643-645 (1985). Toxicology , quantitative analysis 28b

61 153 J.F. HEWETSON, J.G. PACE, J.E. BEHELER, (U.S. Army Medical Research, Inst. of Infectious Diseases, Pathophys.Div. Ft. Detrick, Frederick, MD 21701-5001, USA): Detection and quantitation of T-2 mycotoxin in rat organs byradioimmunoassay. J.A.O.A.C. 70, 654-657 (1987). TLC of T-2 mycotoxin, HT-2, T-2 triol and T-2 tetraol on silicawith 2 sequential solvent systems: 1. chloroform - ethyl acetate - ethanol 50:25:25 and 2. chloroform - ethyl acetate -ethanol 80:10:10. Detection of unlabelled mycotoxin standards by reacting with 4-(p-nitrobenzyl)pyridine; radiolabelledstandards by autoradiography. Toxicology 28b

61 154 P.M. KELLY (Dep. of Science, Suny Coll. of Technology, Box 3050, Marcy Campus, Utica, N.Y. 13504, USA): Separation of amphotericin B components by combined thin-layer and high- performance liquidchromatography. J. Chromatogr. 437, 221-229 (1988). TLC of the major components of amphotericin B on silicawith HPLC-grade methanol. The separated compounds were visualized as contrasting areas under UV 365 nm duringthe evaporation process. Confirmation of the results by reversed-phase HPLC. Pharmaceutical research , qualitative identification 28a

61 155 F.R. KUNZ, H. JORK, (Univ. des Saarlandes, FB Pharmazie u. Biologische Chemie, D-6600 Saarbrücken, FRG): Quantitative fluorometric determination of netilmicin and gentamicines C1, C1a, C2 and C2a in Pharmaceuticalformulations on RP layers. H. TRAITLER, A. STUDER, R.E. KAISER (eds): Instrumental HPTLC, Institute forChromatography, Bad Dürkheim, FRG (1987), 437-451. TLC on C8- or C18-bonded silica with methanol - 0.1 M lithiumchloride in 32 % NH3 5:25. Detection by dipping in DOOB-reagent and heating. Quantification by fluorodensitometryafter dipping in a solution of paraffin oil - n-hexane 1:6. Detection limits, 10 and 50 ng, resp. Pharmaceutical research , quantitative analysis , densitometry , netilmicin , gentamicines 28a

61 156 G. LEHMANN, J. GANZ, G. GRUCZA, (FR Biogeogr., AG Nahrungskettenanalyse, Univ. Saarland, Saarbrücken,F.R.G.): Schnellbestimmung von Natamycin in Käse. (Fast determination of natamycin in cheese.)Lebensmittelchem. Gerichtl. Chem. 41, 12-14 (1987). TLC on silica with acetic acid - butyl acetate - n-butanol - water20:30:80:50. Detection after drying at 110 °C by spraying first with 0.1% aqueous potassium permanganate solutionand after 15 min. with 0.01% aqueous bromophenolblue solution. Detection limit 1 µg. Food analysis , qualitative identification , Pimaricin 28a

61 157 H. OKA*, Y. IKAI, N. KAWAMURA, K. UNO, M. YAMADA, K. HARADA, M. SUZUKI, (*Aichi Prefec. Inst. Public Health,Tsuji-Machi, Kita-Ku, Nagoya 462, Japan): Improvement of chemical analysis of antibiotics XII. Simultaneousanalysis of seven tetracyclines in honey. J. Chromatogr. 400, 253-261 (1987). TLC of residual oxytetracycline,tetracycline, chlortetracycline, doxycycline, methacycline, dimethylchlortetracycline and minocycline in honey on silicaand RP-8 silica with chloroform - methanol - 5% aqueous Na2EDTA 65:20:5 (lower phase) and methanol - acetonitrile -0.5M aqueous oxalic acid 1:1:4 (pH 3.0), resp. Detection by spraying with 0.2M aqueous magnesium chloride and 10%TEA in methanol, resp., and then observing under UV at 360 nm. Detection limits 0.1 ppm in honey. Food analysis , quantitative analysis , tetracyclines , antibiotics 28a

61 158 M. PETZ, R. SOLLY, M. LYMBURN, M.H. CLEAR, (Univ. Münster, Inst. of Food Chem., Piusallee 7, D-4400 Münster,F.R.G.): Thin-layer chromatographic determination of erythromycin and other macrolide antibiotics inlivestock products. J.A.O.A.C. 70, 691-697 (1987). TLC of 4 macrolide antibiotics (erythromycin, tylosin,oleandomycin, spiramycin) on silica with n-butanol - water - acetic acid 60:20:20. Detection after reacting withxanthydrol, limit 0.02 mg/kg. Quantification of results by bioautography using Bacillus subtilis. Food analysis , quantitative analysis , densitometry , qualitative identification 28a

61 159 K. SAITO, M. HORIE, Y. HOSHINO, N. NOSE, H. NAKAZAWA, (Saitama Profct. Inst. Public Health, Urawa, Japan338): (Determination of biozamycin in chicken eggs and meat by fluorescence densitometry and highperformance thin-layer chromatography. ) (Japanese). Jap. Hygienic Chem. 32, 442-446 (1986) (Eisei Kagaku).TLC on silica with acetonitrile - water 7:1. Determination by fluorescence densitometry at 365 nm. Detection limit 0.2µg/g. Quantitative analysis , densitometry , bicozamycin 28a

61 160 J. SAWINSKY-ACSADI*, A. HALASZ, E. TYIHAK, (*KEKI, H-1022 Budapest, Herman Ottó u.15., Hungary): (The



61 160 J. SAWINSKY-ACSADI*, A. HALASZ, E. TYIHAK, (*KEKI, H-1022 Budapest, Herman Ottó u.15., Hungary): (Theapplicability of OPLC technique in detecting mycotoxins in foodstuffs and in fodders. ) (Hungarian). ElelmezésiIpar 41, 324-326 (1987). OPLC of zearalenons on silica with chloroform - acetone 9:1 or with toluene - ethyl acetate -formic acid 30:14:2. Detection by UV 254 nm. Sensitivity 40-60 ng/spot. Spraying with Sarudi reagent (1% 4-methoxy-benzene-diazonium-fluoroborate). Sensitivity 20 ng/spot. Recovery:62.5-84.6%. Agricultural , qualitative identification 28b

61 161 P.M. SCOTT, (Health and Welfare Canada, Health Protection Branch, Ottawa, Ontario K1A 0L2, Canada): Mycotoxins. J.A.O.A.C. 70, 276-281 (1987). A review with many references on mycotoxin analysis by TLC. Agricultural , food analysis , review , mycotoxins 28b

61 162 A. SIMONELLA, L. TORRETI, C. FILIPPONI, A. FALGIANI, L. AMBROSII, (Inst.Zooprofilattico Sperimentaledell^Abruzzo e del Molise „G. Caporale“, Teramo, Italy): Simultaneous determination of aflatoxins G1, B1, G2, B2in animal feedstuffs by HPTLC and RP-HPLC. J. High Resol.Chromatogr. 10, 626-628 (1987). TLC on HPTLC silicawith chloroform - acetone 9:1 in the one-dimensional mode and with benzene - methanol - acetic acid 9:0.5:0.8 andchloroform - acetone 9:1 in the bidimensional mode. Detection under UV; confirmation through formation of derivativesby spraying the plate with 25% aqueous HNO3. Sensitivity 0.05-0.1 ng for G1 and B1, Food analysis , qualitative identification 28b

29. Pesticides and other agrochemicals

61 163 ANONYMOUS: Determination of pesticide residues in food products. (Determination of chlorinated hydrocarbonresidues in food products originating from vegetables.) (Hungarian). MSZ (Hungarian Norm) 14475/1-87, p. 8. TLC of-HCH, p,p’DDT and metabolites on silica with n-heptan with 0.3% ethanol. Visualization with silvernitrate-2-phenoxyethanol. Detection limit: 0.01 µg; recovery: 75-90%. Food analysis , comparison of methods 29a

61 164 E. GRIMM*, S. NEUMANN, B. KRUG, (*Sektion Biowissensch. der Martin-Luther-Univ., Halle (Saale), Germany-DDR): Transport of xenobiotics in higher plants. IV., Amobility of the acidic compounds bromoxynil andpentachlorophenol. Biochemie und Physiologie der Pflanzen 182, 323-332 (1987). Radiochemicals and plant methanolextracts incubated for 2 h were examined by TLC on silica with chloroform - ether - acetic acid 40:9:1 (for bromoxynil)and chloroform - cyclohexane - acetic acid 9:9:2 (for pentachlorophenol). Radioscanning. Agricultural , densitometry 29a, d

61 165 R. KAMAL*, M. MANGLA, (*Univ. of Rajastia, Lab. of Plant Physiology and Biochemistry, Jaipur 302004, India): Rotenoids from Indigofera tinctoria and their bio-efficacy against cyclops, the carrier of Dracunculiaris. Pharmazie 42, 356 (1987). TLC of Rotenoids (terphrosin, sumatrol, rotenone, deguelin, dehydro-deguelin) on silica withchloroform - ether 95:5 or with chloroform - ethyl acetate - acetic acid 196:3:1. Vizualisation by spraying with hydroiodicreagent and heating at 120°C for 20 min. Agricultural , qualitative identification , insecticides 29f

61 166 I.SH. KOFMAN, M.D. LINETSKY, (Plant Physiology Institute, Ukrainian SSR Acad. Sci., 252627, Kiev, USSR): Identification of multicomponent mixtures by spectrodensitometric TLC. H. TRAITLER, A. STUDER, R.E.KAISER (eds): Instrumental HPTLC, Institute for Chromatography, Bad Dürkheim, FRG (1987), 277-284. TLC oftrifluralin and its related compounds on silica with step-by-step chromatography in 5 eluting systems. Identification bydensitometric registration, diffuse reflection electronic spectra denstitometry and differential signal resolutiondensitometry. The solvents used: 1. hexane - dichloroethane 7:3, 2. hexane - diethyl ether 3:1, 3. hexane - toluene 3:1,4. benzene - acetonitrile - formic acid 130:40:1.5, 5. carbon tetrachloride - acetonitrile - formic acid 50:20:1. Agricultural , qualitative identification , trifluralin 29d

61 167 C. MARUTOIU*, M. VLASSA, C. SARBU, S. NAGY, (*Inst. of Chem., 3400 Cluj-Napoca, str Fintinele 30, Romania): Separation and identification of organophosphorus pesticides in water by HPTLC. J. High Resol. Chromatogr.10, 465-466 (1987). TLC of 8 organophosphorus pesticides on silica with petrol ether - benzene - ethyl acetate -60:30:10 and petrol ether - benzene - chloroform - ethyl acetate 60:30:5:5. Detection by spraying with 0.01 %2-methyl-thioacridone in dichloromethane and heating for 15 min at 200 °C, examining under UV 366 and 254 nm.Identification by Rf. Environmental , qualitative identification , organophosphorus pesticides 29b

61 168 V.B. PATIL*, M.T. SEVAIKAR, S.V. PADALIKAR, (*Regional Forensic Sci. Lab., State Maharashtra, Contonment,



61 168 V.B. PATIL*, M.T. SEVAIKAR, S.V. PADALIKAR, (*Regional Forensic Sci. Lab., State Maharashtra, Contonment,Auraugabad-431 002, India): Specific spray reagent for the detection of endosulfan by thin-layerchromatography. J. Chromatogr., 396, 441-443 (1987). TLC on silica with n-hexane - acetone 4:1. Detection byspraying with 2% sodium hydroxide solution followed by 5% aqueous nickel chloride solution - 30% NH3 1:1.Discussion of the mechanism of the color reaction. Greyish black spots were observed immediately, and stable formore than 24 h. Agicultural , qualitative identification , specific spray reagent , endosulfan 29f

61 169 J. SHERMA, (Dep. Chem., Lafayette Coll., Easton, Pennsylvania 18042, USA): Pesticides. Anal. Chem. 59,18R-31R (1987). Review with 18 references on TLC analysis of pesticide residues. Also other techniques. Agricultural , review , pesticides 29

61 170 X. XIAO (Xiao Xuecheng), (Xiangtan Munic. Station Pub. Health Antiepidemics, Xiangtan, Hunan, P.R. China): (Rapidassay of organophosphorus pesticides by thin-layer chromatography). (Chinese). Chinese J. Chromatogr. 5,133, 130 (1987) (Sepu). TLC of organophosphorus pesticide residues on silica with benzene - methanol 9:1. Detectionby spraying with 0.2% palladium chloride. Toxicology , qualitative identification , organophosphorus pesticides 29b

61 171 W. YE (Ye Wenfa), W. MA (Ma Weilin), (Dep. Chem., Cent. China Norm. Univ., Wuhan, P.R. China): (Monitoring ofthe synthesis process of m-phenoxybenzaldehyde by thin-layer chromatography). (Chinese). Chinese J.Chromatogr. 6, 61-62 (1988) (Sepu). TLC on silica with chloroform - petrol ether 3:7 or benzene - petrol ether 45:55.Detection by spraying with potassium permanganate - sulfuric acid reagent. Agricultural , quantitative analysis , m-phenoxybenzaldehyde 29f

61 172 H. ZENG (Zeng Hanhe), K. LU (Lu Kangquan), (Hubei Export Import Commodity Insp. Bur., Hubei, P.R. China): Determination of polyphosphate in meat products by thin-layer chromatography) (Chinese). Chinese J. Food &Ferm.Ind. 2, 47-52 (1987) (Shipin Yu Fajiao Gongye). TLC on cellulose with isopropanol - 13.5% trichloro acetic acid -25% NH3 140:40:0.6. Detection by spraying with tin chloride reagent. Quantification by densitometry at 780 nm. Food analysis , quantitative analysis , densitometry , polyphosphate 29b

30. Synthetic and natural dyes

61 173 A. ACZEL, (Szeged Papika-processing Comp., H-6701 Szeged, P.O. Box 114, Hungary): The overpressuredthin-layer chromatographic separation of capsanthin-capsorubin. Fresenius Z. Anal. Chem. 330, 462 (1988). TLCand OPTLC on silica with petrol ether - benzene - acetone - acetic acid 80:10:4:5 and methanol - acetone - water20:4:3. Quantification by spectrophotometry after elution (capsanthin at 484 nm, capsorubin at 486 nm. Agricultural , quantitative analysis 30b

61 174 A. ACZEL, (Szeged Papika-processing Comp., H-6701 Szeged, P.O. Box 114, Hungary): Application ofoverpressure layer chromatography in red pepper analysis. Study of the carotenoids giving the red colour toground red pepper. Acta Alimentaria 17, 37-41 (1988). OPLC of capsanthin and capsorubin on silica with petrol ether -benzene - acetone - acetic acid 40:10:2:2.5. Quantification by spectrophotometry after elution. Recoveries: capsanthin95,2% ± 2,99%, capsorubin 91,8% ± 3.88%. Agricultural , preparative TLC 30b

61 175 ANONYMOUS: Changes in methods - color additives FD&C color additives in foods - rapid cleanup forspectrophotometric and thin layer chromatographic identification. J.A.O.A.C. 71, 213-215 (1988). TLC of YellowNo. 6, Red No. 40, Blue No.1, Green No. 3 on silica with n-butanol - MEK - NH3 - water 5:3:1:1.

30a

61 176 H.G. DAOOD*, P.A. BIACS, A. HOSCHKE, M. HARKAY-VINKLER, F. HAJDU, (*Central Food Res. Inst., H-1022Budapest, Herman O. ut 15, Hungary): Separation and identification of tomato fruit pigments by TLC andHPTLC. Acta Alimentaria 16, 339-350 (1987). TLC and HPTLC of carotene, neurosporin, lycopene, polycopene,violaxanthin, pheophytin, neoxanthin, cis-mutatoxanthin, lutein on silica with first hexane - benzene - acetone - aceticacid 80:10:5:5 and second hexane - benzene - acetone 90:8:2. Agricultural , qualitative identification 30b

61 177 P.B. HOJ, B.L. MOLLER, (Dep. Plant Physiol. & Chem. Inst., Royal Veterinary & Agricol. Univ., 40 Thorvaldsensvej,DK-1871 Frederiksberg C, Denmark): Acid-labile sulfide and zero-valence sulfur in plant extracts containingchlorophyll and ionic detergents. Anal. Biochem. 164, 307-314 (1987). TLC on silica with chloroform - methanol -water 78:21:1. Environmental , qualitative identification , acid-labile sulfide , zero-valence sulfur , chlorophyll , detergents 30b, 35a



61 178 E. JANOS*, E. TYIHAK, (*Plant Protection Inst., Hungarian Acad. of Sci., H-1525 Budapest, Herman Ottó u.15,Hungary): Determination of chlorophylls and other pigments in different paprika varieties by planarchromatography. Proc.Scientific International Technical-Development Symposium on Hungarian Paprika, 1987,September 18. Kalocsa, p. 497. OPTLC of 9 plant pigments (carotenoids, chlorophylls, xantophylls) on silica withbenzene - isopropanol - water 100:12:0.25. Qualitative and quantitative evaluation by densitometry. Agricultural , densitometry 30b

61 179 H. LIU (Liu Haucheng), X. MO (Mo Xianbin), X. CHEN (Chen Xueqing), (Dep. Chem. Eng., Zhejiang Coll. Tech.,Hangzhou, P.R. China): (Determination of alizarin by thin-layer chromatography/spectrophotometry). (Chinese).Chinese J. Chem. Reag. 9, 371-374 (1987) (Huaxue Shiji). TLC on silica with benzene - carbon tetrachloride - aceticacid - acetone 50:75:0.8:3.8. Quantification by spectrophotometry at 425 nm after elution with acetone. Quality control , quantitative analysis , alizarin 30a

61 180 J. NI (Ni Jianhua), H. ZHOU (Zhou Huilan), W. WANG (Wang Xingguo), (Lanzhou Univ., Lanzhou, P.R. China): (Determination of pigments in green plant leaves by using thin-layer chromatography). (Chinese). J. LanzhouUniv., Nat. Sci. 23, 109-113 (1987) (Lanzhou Daxue Xuebao, Ziran Kexueban). TLC of 9 pigments (neoxanthin,violaxanthin, antheraxanthin, lutein, chlorophyll b, chlorophyll a, cryptoxanthin, pheophytins, ß-carotene) on silica withbenzene - tert.butanol 9:10. Quantification by densitometry. Agricultural , pigments 30b

61 181 N. SUZUKI*, K. SAITOH, K. ADACHI, (*Dep. Chem., Fac. Sci., Tohoku Univ., Sendai 980, Japan): Reversed-phasehigh-performance thin-layer chromatography and column liqiud chromatography of chlorophylls andderivatives. J. Chromatogr. 408, 181-190 (1987). TLC of six chlorophylls and derivatives on RP-18 silica with singlesolvents including alcohols, acetone, acetonitrile, and binary solvents such as acetone - acetonitrile and ethanol - water.Detection by densitometry at 410 nm and 435 nm. Discussion of the retention behaviours. Also, HPLC. Agricultural , densitometry , chlorophylls and derivatives 30b

61 182 A. VALIN*, R. CASTILLO, G. NEGRE-SADARGUES, R. LENEL, (*Laboratoire de Physiologie des Invertébrés, Univ.des Sciences et Techniques du Languedoc, Place E. Bataillon, 34060 Montpellier Cedex, France): Quantitativeaspects of carotenoid pigment circadian variations in the crayfish Astacus leptodactylus. BiochemicalSystematics and Ecology 15, 607-610 (1987). TLC of the carotenoid pigments of whole animal, epidermis andhepatopancreas of the crayfish on silica with petrol ether - methanol 95:5 and benzene - ethanol - methanol - 17:2:1.Identification by means of several criteria: Rf-values, chromatographic comparisons, visible absorption spectra of thefractions eluted from TLC plates. Agricultural , qualitative identification 30b

61 183 Y. ZHOU (Zhou Yingdi), K. GAO (Gao Kunyu), L. CHENG (Cheng Lubai), (Dep. Chem. Eng., Dalian Inst. Technol.,Dalian, P.R. China): (Determination of bromamine acid by thin-layer chromatography). (Chinese). Chinese J.Chromatogr. 6, 121-123 (1988) (Sepu). TLC of bromamine acid and its impurities on silica with ethyl acetate -ethylhexanol - acetic acid - water 2:1:1:1, and ethyl acetate - petrol ether 1:5. Quantification by densitometry at 260 nm. Quality control , quantitative analysis , densitometry , bromamine acid 30a

61 184 M. ZSUGA*, A. KISS, (*Departm. of Applied Chem., Kossuth Lajos University, H-4010 Debrecen, P.O. Box 10,Hungary): Alkaline degradation of parent chromonoid compounds (chromone, flavone, isoflavone). ActaChimica 124, 485-489 (1987). TLC of chromonoid compounds and their degradation products on silica with benzene -ethanol 9:1. Detection under UV 254 and 366 nm. Also spraying with 1% FeCl3x6H2O in 80% aq. ethanol solution,concentrated sulfuric acid. Detection limit 0.1-0.8 µg/mL. Agricultural , qualitative identification 30b

32. Pharmaceutical and biomedical applications

61 185 ANONYMOUS: Changes in methods - dexamethasone in Drug substances and elixirs. Quantitative andidentification methods. J.A.O.A.C. 71, 220-222 (1988). TLC of dexamethasone on silica with chloroform - diethyl amine2:1 or chloroform - acetone - glacial acetic acid 80:40:1. Detection under UV 254 nm. Pharmaceutical research , quality control 32a

61 186 F. BAKIAS*, I. TÖRÖK, GY. KELEMEN, T. PAAL, (*National Pharmaceut. Inst., H-1051 Budapest, Zrinyi u. 3.,Hungary): New methods for the simultaneous determination of 131I-hippuran and its expected maincontaminant after previous separation. (Hungarian). Magyar Kémiai Folyóirat 93, 277-281 (1987). TLC oforthoiodo-hippuric acid and its main contaminants on silica with methanol - chloroform - acetic acid 100:75:2.5.Densitometry at 254 nm. Pharmaceutical research , densitometry 32b



61 187 R. BAUER, I.A. KHAN, H. WAGNER*, (*Inst. f. Pharmazeutische Biol. der Univ. München, Karlstrasse 29, D-8000München 2, F.R.G.): Echinacea - Nachweis einer Verfälschung von Echinacea purpurea (L. ) Mönch mitParthenium integrifolium L. (Echinacea. Prove of an adulteration of Echinacea purpurea (L.) Mönch with Partheniumintegrifolium L.) Dtsche.Apoth.Ztg. 127, 1325-1330 (1987). TLC of sesquiterpene esters and polyacetylene compoundson silica with toluene - ethyl acetate 7:3. Detection by spraying with vanillin/sulfuric acid reagent. TLC of caffeic acidderivatives on silica with ethyl formate - formic acid - water - toluene 100:10:10:5. Detection by spraying with naturaldye reagent/polyethylene glycol. Pharmaceutical research , qualitative identification 32e

61 188 R. BAUER, P. REMIGER, H. WAGNER*, (*Inst. f. Pharmazeutische Biol. der Univ. München, Karlstrasse 29, D-8000München 2, F.R.G.): Echinacea - Vergleichende DC- und HPLC-Analyse der Herba- Drogen von Echinaceapurpurea, Echinacea pallida und Echinacea angustifolia. (Echinacea. Comparative TLC and HPLC analysis of theherba drugs of Echinacea purpurea, E. pallida and E.angustifolia.) Dtsch. Apoth. Ztg. 128, 174-180 (1988). TLC ofisobutylamide on silica with hexane - ethyl acetate 2:1. Detection under UV and with anisaldehyde sulfuric acid reagent(= methanol - glacial acetic acid - anisaldehyde - conc. sulfuric acid 85:10:0.5:0.5) and heating for 5 min. at 100 °C. TLCof caffeic acid derivatives on silica with ethyl formate - formic acid - water - toluene 100:10:10:5. Detection under UV at254 nm and with natural dye reagent/polythylene glycol. Pharmaceutical research , qualitative identification 32e

61 189 S.K. BAVEJA, S. SINGH, (Div. Pharm., Dep. Pharm. Sci., Panjab Univ., Chandigarh 160 014, India): Thin-layerchromatographic examination of the degradation of centbucridine in aqueous solutions. J. Chromatogr. 396,337-344 (1987). TLC of centbucridine and its major degradation products on silica with six solvent systems. Detectionunder UV 254 nm, spraying first with Dragendorff’s reagent, then with 25% sulfuric acid and exposing to iodine vapour.Identification by comparison of their Rf values, fluorescent nature and color reactions with those of authentic materials. Pharmaceutical research , qualitative identification , centbucridine (9-n-butylamino-1 , 2 , 3 ,4-tetrahydrocridine)

32c

61 190 W. BERNHARD*, A.D. FUHRER, A.N. JEGER, S.R. RIPPSTEIN, (*Inst. für Gerichtschemie, Pestalozzistr. 22, CH-4012Basel, Switzerland): Nachweis und die Bestimmung von Betarezeptorenblockern in Urin mittels instrumentellerDünnschicht-Chromatograhie. Detection and determination of some beta-receptor blockers in urine by instrumentalTLC. Fresenius Z. Anal. Chem. 330, 458-459 (1988). TLC on silica with ethyl acetate - methanol (saturated with formicacid) - water 800:200:1, two runs. Densitometry by fluorescence 366/>410 nm. Additional identification by 3 sprayreagents: 1) 0.5 % fast blue B in water, 2) 0.2 % naphthoquinone-1,2- sulfonic acid Na salt in ethanol - water 1:1 and3) formaldehyde in sulfuric acid followed by heating at 110°C. Acebutolol, Alprenolol, Atenolol, Carazolol, Labetolol,Metoprolol, Oxprenolol, Propranolol and Sotalol can be identified by different colors. Screening method for lowconcentrations of beta-blockers (reaction chromatography): 100 µL urine extract sprayed onto HPTLC silica;overspraying with dansylchloride in acetone, then with sodiumhydrogenecarbonate in water; heating for 15 min at120 °C; then chromatographic development with acetone - chloroform 9:1. Evaluation by densitometry (fluorescence366/>410 nm); detection limit 10 ng. The method of analysis is available as CAMAG Application booklet (inpreparation). Clinical chemistry research , quantitative analysis , densitometry , doping analysis , beta-blockers 32b

61 191 TH. BEYRICH*, E. NEUBAUER, (*Sektion Pharmazie der Ernst-Moritz-Arndt-Univ., DDR-2200 Greifswald): Hydrolysevon Nitroarylestern unter dem Einfluss von Pilocarpin, Histamin, Cimetidin, 2-Methylimidazol sowie Piperazinund Allopurinol als Katalysatoren. Hydrolysis of nitroarylesters under the influence of pilocarpine, histamine,cimetidine, 2-methylimidazole, piperazine and allopurinol as catalysts. Pharmazie 42, 824-826 (1987). TLC of2-nitrophenyl acetate, 2-nitro-4-methylphenyl acetate, 2,4-dinitrophenyl acetate, 4,6-dinitro-2-methylphenyl acetate onsilica with n-hexane - benzene - ethyl acetate - chloroform 340:67:40:20. Detection with iodine, ethanolic KOH andunder UV. Pharmaceutical research , qualitative identification 32b

61 192 M. BIDLO-IGLOY, S. SZABO, (Institute for Drug Research, P.O.B. 82., H-1325 Budapest, Hungary): Simultaneousdetermination of some chemically related compounds of pharmaceutical interest by quantitative TLC. H.TRAITLER, A. STUDER, R.E. KAISER (eds): Instrumental HPTLC, Institute for Chromatography, Bad Dürkheim, FRG(1987), 61-69. TLC of chemically related compounds (e.g. isomers) on silica with 1) dichloromethane (presaturationwith NH3), 2) chloroform (presaturation with NH3), and 3) ethyl acetate. Detection under UV at 254 nm and sprayingwith o-tolidine reagent after chlorination. Quantification by densitometry. Quality control , quantitative analysis , densitometry , chemically related compounds , isomers 32a

61 193 J. BOOKER, (Analytical Res. Lab., Kimbley-Clark Corporation, Neenah, Wisconsin, USA - 54956): Degradation



61 193 J. BOOKER, (Analytical Res. Lab., Kimbley-Clark Corporation, Neenah, Wisconsin, USA - 54956): Degradationproducts of irradiated haloperidol: implications for the development of an implantible delivery system. Pharmazie 43, 31-32 (1988). TLC of haloperidol and its degradation products on silica with p-dioxane - benzene -ethanol - NH3 50:40:7:3. Visualization under UV. Pharmaceutical research , qualitative identification 32c

61 194 E.A. BUNCH, (Food and Drug Admin., 909 First Ave., Seattle, WA 98174): Liquid chromatographic determinationand identification tests for dexamethasone in bulk drugs and elixirs: collaborative study. J.A.O.A.C. 70,967-973 (1987). TLC of dexamethasone on silica with chloroform - diethyl amine 2:1 or chloroform - acetone - aceticacid 80:40:1. Location of spots under shortwave UV. Pharmaceutical research , quality control , qualitative identification 32a

61 195 TH. CACHET, E. ROETS, J. HOOGMARTENS*, H. VANDERHAEGHE, (*Katholieke, Univ. Leuven, Lab. voor Farm.Chem., Inst. voor Farm. Wetenschappen, Van Evenstraat 4, B-3000 Leuven, Belgium): Separation of novelderivatives from commercial erythromycin samples by thin-layer chromatography. J. Chromatogr. 403, 343-349(1987). TLC on silica with 1. ethyl acetate - methanol - 25 % NH3 85:10:5, 2. ether - methanol - 25 % NH3 90:9:2,3. dichloromethane - methanol - 25 % NH3 90:9:1.5, and 4. diisopropyl ether - methanol - 25 % NH3 75:35:1. Detectionby drying at 110 °C for 5 min., spraying with 4-methoxybenzaldehyde - sulfuric acid - ethanol 1:1:9 and heating againfor 1 min. Pharmaceutical research , erythromycins , erythromycin derivatives 32c, 28a

61 196 CHUN-BO AI*, LIAN-NIANG LI, (Institute of Materia Medica, Chinese Ac. of Med. Sciences, 1 Xian Nong Tan Street,Beijing, China): Stereostructure of salvianolic acid B and isolation of salvianolic acid C from Salvia mittiorrhiza. Journal of Natural Products 51, 145-149 (1988). TLC of salvianolic acid A and salvianolic acid C on silica withchloroform - methanol - formic acid 85:15:1. Detection under UV at 254 nm. TLC of dimethyl heptamethylsalvianolate Bwith chloroform - methanol - formic acid 100:0.5:0.5. Identication by blue fluorescence. Pharmaceutical research , qualitative identification 32e

61 197 T.D. CRY, F. MATSUI, R.W. SEARS, N.N. CURRAN, E.G. LOVERING, (Health and Welfare Canada, HealthProtect.Branch, Bureau of Drug Research, Ottawa, Ontario K1A OL2, Canada): Liquid chromatographic methods forassay of carbamazepine, 10,11-dihydrocarbamazepine, and related compounds in carbamazepine drugsubstances and tablets. J.A.O.A.C. 70, 836-840 (1987). TLC of iminodibenzyl and iminostilbene in carbamazepinedrug substances and tablets on silica with toluene. Detection under UV 254 nm and by spraying with 0.5% potassiumdichromate in 20% aqueous sulfuric acid. Detection limits 0.05 µg each. Pharmaceutical research , quality control , qualitative identification 32a

61 198 E. DELLA CASA, G.MARTONE, (Inst. Forensic Med. Dept. Toxicol., Univ. Napoli, Italy): A quantitative densitometricdetermination of heroin and cocaine samples by high-performance thin-layer chromatography. Forensic Sci.Internat. 32, 117-120 (1987). HPTLC determination of heroin and cocaine on silica with cyclohexane - benzene - diethylamine 70:25:10 resp. chloroform - methanol 50:50. Quantification by densitometry (absorbance) at 281 nm for heroinand at 233 nm for cocaine. Comparison with GC shows reliability of TLC method. Toxicology , quantitative analysis , densitometry 32c

61 199 A. DUCZ, B. MARX, K. MIHALYFI, Z. VEGH*, (*Pharmaceutical Factory Köbánya, Budapest, Hungary): Phytochemical study of the active principles of Bitter tincture. (Hungarian). Acta Pharm. Hungarica 57, 209-217(1987). TLC of absinthin, foliamenthin, genciopicrozide, naringin, neohesperidin on silica with acetone - chloroform -water 80:20:5 or 145:40:15. Detection after spraying with 1% vanillin solution or 5% H2SO4 methanolic solution underUV 254 and 366 nm. Densitometry. Clinical chemistry research , densitometry 32e

61 200 J. GE (Ge Jianhua), Y. HE (He Yi), (Dep. Anal. Chem., China Pharm. Univ., Nanjing, P.R. China): (Determination ofemodin and indirubin in danggui longhui pills by thin-layer chromatography). (Chinese). J. China Pharm. Univ.18, 95-98 (1987) (Zhongguo Yaoke Daxue Xuebao). TLC on silica with benzene - ethyl acetate - formic acid - water60:20:1:10. Extraction with methanol or chloroform. Quantification by spetrophotometry at 435 nm and 550 nm, resp. Pharmaceutical research , quantitative analysis , emodin , indirubin 32e

61 201 R.K. GILPIN*, L.A. PACHLA, (Dep. Chem., Kent State Univ., Kent, Ohio 44242, USA): Pharmaceuticals and relateddrugs. Anal. Chem. 59, 174R-197R (1987). A review with many references on TLC application in the analysis ofpharmaceuticals and related drugs. Also other techniques. Review , pharmaceuticals and related drugs 32

61 202 B. GÖBER*, H. LISOWSKI, P. FRANKE, (*Sektion Chemie der Humboldt Univ. zu Berlin, Goethestr. 54, DDR-1120



61 202 B. GÖBER*, H. LISOWSKI, P. FRANKE, (*Sektion Chemie der Humboldt Univ. zu Berlin, Goethestr. 54, DDR-1120Berlin): Zur Stabilität vom Bromhexin und zur Struktur seiner Abbauprodukte. Stability of bromhexine and thestructure of its degration products. Pharmazie 43, 23-26 (1988). TLC of 3-cyclohexyl-6,8-dibromochinazoline-4-one,3-cyclohexyl-6,8-dibromochinazo-line and 3-amino-4,6-dibromobenzaldehyde on silica with chloroform - hexane -methanol - acetic acid 40:40:10:10 and benzene - methyl acetate - methanol - NH3 50:30:20:1.5. Detection withpotassiumtetraiodobismutate(II) and/or under UV at 254 nm. Detection limits 0.5-2.0 µg. Pharmaceutical research , qualitative identification 32b

61 203 H.O. GÜNTHER, (Labor Dr. S. Ehrenstorfer, Gögginger Str.78, D-8900 Augsburg, F.R.G.): Zur Bestimmung undDifferenzierung der essentiellen Inhaltsstoffe in Hopfen mit DC, HPLC und IR. (Determination and differentationof the essential ingredients in hops with TLC, HPTLC and IR.) MERCK Spectrum 1, 54-56 (1988). TLC of humulon,lupulon, xanthohumol and chlorophyll on silica with cyclohexane - ethyl acetate - propionic acid 60:38:2. Detectionunder UV at 365 nm. Agricultural , qualitative identification 32e, 28

61 204 N. HAAGSMA, E.R. BATHELT, J.W. ENGELSMA, (Dep. of the Science of Food of Animal Origin, Fac. of Vet. Med.,Univ. of Utrecht, P.O. Box 80175, NL-3508 TD Utrecht): Thin-layer chromatographic screening method for thetranquillizers azaperone, propiopromazine and carazolol in pig tissues. J. Chromatogr. 436, 73-79 (1988).Screening for tranquillizer residue in pig muscle, liver and kidney tissue by HPTLC on silica (two-dimensional) with 1.dichloromethane - acetone - 25% NH3 100:100:5 and 2. n-butanol - acetic acid - water 80:20:100 (organic layer).Detection under UV 254/366 nm. Detection levels were 25 µg/kg for propiopromazine, 50 µg/kg for azaperone and 125µg/kg for carazolol. Agricultural , quality control , qualitative identification 32d

61 205 M.O. HAMBURGER*, G.A. CORDELL, (*Program for Collaborative Research in the Pharm. Sci., Univ. of Illinois atChicago): A direct bioautographic TLC assay for compounds possessing antibacterial activity. Journal ofNatural Products 50, 19-22 (1987). TLC on silica with chloroform - methanol 98:2 for stigmasterol, sitosterol andpalmitic acid, chloroform - methanol 80:20 for quercetin and p-coumaric acid, isopropanol - ethyl acetate - water70:20:10 for tannic acid and sucrose, methanol - acetone 50:50 for penicillin, chloroform - methanol 90:10 forchloramphenicol, chloroform - ethanol - hexane - ethyl acetate 18:6:42:34 for the lignans, chloroform - ethanol - ethylacetate - hexane 70:10:17:3 for the quassinoids and THF - dichloromethane 25:75 for the camptothecines. Platesincubated in humid atmosphere overnight; sprayed with any of the aqueous solutions 1)2,3,5-triphenyltetrazoliumchloride, 2) p-iodo-nitrotetrazolium violet, 3) tetranitroblue tetrazolium or, 4)thiazolyl-thiazolium; plates incubated for another 4 hours at 37°C; plates treated with ethanol - water 7:3 beforeevaluating the bioautogram vizually. Pharmaceutical research , qualitative identification 32c

61 206 X. HE (He Xinliang), (Shandong Provincial Inst. for Drug Control, Jinan, P.R. China): (Determination of totalsaponins in fiveleaf gynostemma, Gynostemma pentaphyllum. ) (Chinese). J. Chinese Herb Med. 18, 447-448(1987) (Zhong Caoyao). TLC on silica with butanol - ethyl acetate - water 4:1:5. Detection by spraying with sulfuric acid- methanol 1:1, heating at 105 °C and observing under UV. Pharmaceutical research , quantitative analysis , saponins 32e, 14

61 207 X. HE (He Xinliang), (Shandong Provincial Inst. for Drug Control, Jinan, P.R. China): (Study of the ginsenocides indifferent varieties of ginseng). (Chinese). J. Chinese Herb Med. 19, 93 (1988) (Zhong Caoyao). TLC on silica withbutanol - ethyl acetate - water 4:1:5. Detection by spraying with sulfuric acid - methanol 1:1 and heating at 105 °C.Comparison of the chromatograms under UV at 365 nm. Pharmaceutical research , qualitative identification , ginsenocides 32e, 14

61 208 SH. HOU (Hou Shexiang), G. LIAO (Liao Gongtie), H. YANG (Yang Huayuan), S. LI (Li Suzheng), (School Pharm.,West China Univ. Med. Sci. Chengdu, Sichuan, P.R. China): (Studies on quality control method of compounds„Sansheng“ injection. ) (Chinese). J.Chinese Herb Med. 18, 349- 352 (1987) (Zhong Caoyao). TLC of alkaloids,triterpenoidal saponin, essential oil, polysaccharides, lipids on silica with chloroform - methanol - water 13:7:2 foralkaloids and triterpenoidal saponin, and petrol ether - ethyl acetate 15:5 for essential oil. Detection by spraying withpotassium iodobismuthate, 10% sulfuric acid, 5% vanillin - sulfuric acid, resp. Pharmaceutical research , quality control , qualitative identification , alkaloids , triterpenoidalsaponin , essential oil.

32e, 14, 15b, 22

61 209 Y. HUI (Hui Yueming*), Y. HU (Hu Yuehong), Y. WU (Wu Yaoping), ZH. HU (Hu Zhibi), D. NI (Ni Dexiang), (*Shanghai



61 209 Y. HUI (Hui Yueming*), Y. HU (Hu Yuehong), Y. WU (Wu Yaoping), ZH. HU (Hu Zhibi), D. NI (Ni Dexiang), (*ShanghaiColl. Trad. Chinese Med., Shanghai, P.R. China): (Studies on tissues culture of common andrographis,Andrographis paniculata. I. The culture of Callus and the analysis of active components) (Chinese). J. Chinese HerbMed. 18, 367-370 (1987) (Zhong Caoyao). TLC of andrographolide and neo-andrographolide on silica with chloroform -ethanol 4:1 and ethyl acetate - ethanol 6:1. Detection by exposing to iodine vapor, or spraying with sulfuric acid andKedde reagent. Pharmaceutical research , quantitative analysis , andrographolide , neo-andrographolide 32e

61 210 C. HUNG, TH. JIRA*, M. SCHLENDER, (*Fr.-L.-Jahn-Strasse 17, DDR-2200 Greifswald): Zur Analyse einigerHauptinhaltsstoffe der Salbe „Sao vang“ mittels quantitativer DC and GC. (Analysis of some main ingredients ofthe „Sao vang“ ointment by means of quantitative TLC and GC.) Pharmazie 42, 58 (1987). TLC of menthol andcamphor on silica with benzene - ethyl acetate 85:15. Detection by spraying with anisaldehyde reagent. Quantificationafter elution with methanol. Pharmaceutical research , quantitative analysis 32e

61 211 H. JORK, (Univ. Saarland, FB Pharm. and Biol. Chem., D-6600 Saarbrücken, FRG): Quantitative HPTLC in the fieldof pharmaceutical applications. H. TRAITLER, A. STUDER, R.E. KAISER (eds): Instrumental HPTLC, Institute forChromatography, Bad Dürkheim, FRG (1987), 193-239. A review with 266 references on TLC/HPTLC separation anddetermination of active substances in pharmaceutical formulations, medical drugs and body fluids with numerousexamples of applications. Discussion of the trend in the field. Review , pharmaceutical formulations , medical drugs , body fluids 32

61 212 TH. KARTING, (Inst. für Pharmakognosie, Univ. Graz, Graz, Austria): Procyanidin- und Flavonoidgehalte vonCrataegus-Drogen in Abhängigkeit von Herkunft, Erntezeitpunkt und Trocknungsform. (Procyanidine andflavonoid contents of Crataegus drugs in dependency on origin, harvest-time and method of drying.) Herba Hungarica26, 15-34 (1987). TLC on silica with ethyl acetate - methanol - water 20:7:3 for the oligomeric procyanidines and ethylacetate - methanol - water 20:5:3 for the polymeric procyanidines. Detection with 1 g vanillin in 100 g 50 % phosphoricacid, heating at 110 °C for 10 min. Identification of the procyanidines under visible light as red spots. Agricultural , qualitative identification 32e

61 213 ZS. KERNOCZI*, E. HETHELYI, B. DANOS, P. TETENYI, (*Research Inst. for Medicinal Plants, Budakalász, Hungary): Presence of carlina oxide in plants of Hungary. Stabilization and antimicrobial effect. Acta Pharm. Hungarica 57,171-181 (1987). TLC of essential oils and carlina oxide on silica with benzene - ethyl acetate or n-hexane. Sprayingwith an ethanolic solution of 1% vanillin and 10% sulfuric acid and heating at 105 °C. Detection under UV 254 nm. Pharmaceutical research , qualitative identification 32e, 15b

61 214 N.S. KHALAF*, F.A. KORA, N.E. FODAH, A.M. EL-NAGGAR, (*Chemistry Departm., Fac. of Science, Al- Azhar Univ.,Cairo, Egypt): Synthesis and antimicrobial activity of some new 3-(4,5-dichlorophthalimido) succinoylaminoacid derivatives. Acta Pharm. Jugoslavica 37, 165-173 (1987). TLC of title compounds on silica with benzene - ethylacetate 1:1. Detection by spraying with iodine-potassium iodide. Pharmaceutical research , qualitative identification 32a, 19

61 215 K. KIEC-KONONOWICZ*, A. ZEJC, G. CHLON, E. STYPULA, J. KRUPINSKA, (*Departm. of PharmaceuticalChemistry, Medical Academy, 30-065 Kraków, Poland): Synthesis of1-(3-amino-2-propanolo)-5,5-diphenyl-hydantoin derivatives as potential antiarrhythmic agents. Acta Pharm.Jugoslavica 37, 123-130 (1987). TLC on silica with benzene - acetone 40:3 or n-butanol - formic acid - water 7:2:2 orethyl acetate - chloroform 1:1. Detection under UV 254 nm. Pharmaceutical research , qualitative identification 32a

61 216 A. LI (Li Anjuan), X. WANG (Wang Xiaomin), H. FENG (Feng Hailong), (Hubai Inst. Drug Cont., Wuhan, P.R. China): (Determination of sinomenine in Qingfengteng, Sinomenium acutum and S. acutum varcinereum, by thin-layerchromatography). (Chinese). Bulletin Chinese Trad. Med. 12, 332-334 (1987) (Zhongyao Tongbao). TLC on silica withbenzene - ethyl acetate - diethyl amine 7:2:1. Quantification by densitometry at 263 nm. Pharmaceutical research , quantitative analysis , densitometry , sinomenine 32e, 22

61 217 G. LU (Lu Guibao), J. FANG (Fang Jin), Q. HUANG (Huang Qiaoshu),(Tianjin Inst. Drug Cont., Tianjin, P.R. China): (Determination of dryocrassin in ferns by thin-layer chromatography). (Chinese). Chinese J. Pharm. Anal. 8,17-20 (1988) (Yaowu Fenxi Zazhi). TLC on silica with n-hexane - chloroform - methanol 30:15:1. Detection by sprayingwith 0.1% fast blue B and heating at 40 °C for 1 h. Quantification by densitometry at 490 nm. Pharmaceutical research , quantitative analysis , densitometry , dryocrassin 32e

61 218 Y. MA (Ma Yuqi), J. XIAO (Xiao Jianchu), T. Li (Li Tong), B. Shen (Shen Baolian), Y. Huang (Huang Yongjie), (Zhong



61 218 Y. MA (Ma Yuqi), J. XIAO (Xiao Jianchu), T. Li (Li Tong), B. Shen (Shen Baolian), Y. Huang (Huang Yongjie), (ZhongShan Med. Univ., Guangzhou, P.R. China): (Pharmacokinetics studies of 3H- sanchi ginseng saponin - Rg1 inrats. ) (Chinese). J. Chinese Herb Med. 18, 405-408 (1987) (Zhong Caoyao). TLC of 3H-Rg1 on silica with chloroform- methanol - water 65:35:10. Detection by spraying with 5% sulfuric acid - methanol and heating at 100-110 °C for5 min. Quantification by scintillation counting after elution. Pharmaceutical research , quantitative analysis , saponin Rg1 32b, 14

61 219 B. MEIER*, D. LEHMANN, O. STICHER, A. BETTSCHART, (*Pharmaz. Inst. ETH Zürich, ETH-Zentrum, CH-8092Zürich, Switzerland): Salicylate in Arzneipflanzen. Screening-Methoden [HPLC, DC) zum Nachweis. (Salicylates inmedicinal plants; HPLC, TLC methods for identification.) Dtsch. Apoth. Ztg. 127, 2401-2407 (1987). TLC of salicinderivatives on silica with ethyl acetate - methanol - water 77:13:10 and methylene chloride - ethyl acetate - methanol -water 50:30:20:5. Detection by spraying with 10% sulfuric acid in ethanol and heating for 5 min. at 110°C or with 0.8 gvanillin in 40 mL acetic acid and heating 5- 10 min. at 100 °C. Detection limit about 0.1%. Pharmaceutical research , qualitative identification 32e

61 220 A.S. MELLIDIS*, P. PAPAGEORGION, (*Lab. of Organic Chemistry, Univ. of Thessaloniki, Thessaloniki 54006,Greece): Naphthazarins from Onosma heterophylla. Journal of Natural Products 50, 618-619 (1987). TLC of5,8-dihydroxy-2-(1'-ß,ß-dimethyl-acryloyloxy-4'-acetoxy-4'-methyl)pentyl-1,4-naphthalenedione on silica with benzene -chloroform - acetone 50:50:1. Agricultural , qualitative identification 32e

61 221 M. MELZACKA*, L. DANEK, A. ADAMUS, (*Inst. of Pharmacology, Polish Ac. of Sciences, 31-343 Kraków, Smetna 12,Poland): Hepatic metabolism of imipramine after prolonged administration of the drug to rats. An in vivo study.Polish Journal of Pharmacology and Pharmacy 39, 379-386 (1987). TLC of imipramine, desipramine and their2-hydroxy homologues on silica with chloroform - isopropanol 10:1, intermediate drying and isopropanol - chloroform -water - 25 % NH3 50:30:20:2; localizing spots under 254 nm UV after spraying with water; quantification byspectrophotometry after elution. Determination limit 0.5 µg/sample. Pharmaceutical research , quantitative analysis 2b

61 222 G. MUSUMARRA, G. SCARLATO, G. ROMANO, G. CAPELLO, S. CLEMENTI, G. GIULIETTI, (Dipt. Sci. Chim., Univ.Catania, Italy): Qualitative organic analysis. Part 2. Identification of drugs by principal components analysis ofstandardized TLC data in four eluent systems and of retention indices on SE 30. J. Anal. Toxicol. 11, 154-163 (1987).TLC identification method for 277 drugs via principal components (PC) analysis of standardized Rf-values in four eluentsystems (ethyl acetate - methanol - 30% NH3 85:10:15:, cyclohexane - toluene - diethyl amine 65:25:10, ethyl acetate -chloroform 50:50, and acetone. After chromatography, plate dipped in potassium hydroxide solution). Toxicology , qualitative identification 32d

61 223 H. NEUNINGER, (Kriminaltechnische Zentralstelle, Abt.II/11, des Bundesministeriums für Inneres Wien, RossauerLände 1, A-1090 Wien, Austria): Nachweis und Identifizierung von Phenylethylaminen (Stimulantien undHalluzinogene). Detection and identification of phenethylamines (stimulants and hallucinogenes). Sci. Pharm. 55,1-11 (1987). Method for the rapid identification of phenethylamine. Color reactions with 4 reagents: a) formaldehyde -sulfuric acid (Marquis), b) ammoniummetavanadate - sulfuric acid (Mandelins), c) ammoniummolybdate - sulfuric acid(Fröhde), d) nitric / sulfuric acid as preliminary group identification preceding TLC. TLC with 6 separation systems, 4using silica, 2 RP-18 layers, with 6 solvent systems. Postchromatographic derivatization with 5 systems selectedaccording to layer material and previous observations. Results of the identification procedure presented in the form oftables. Toxicology , qualitative identification , color reactions 32d

61 224 E. NOVAKOVA, (Inst.für Toxikologie FUL UK, CS-12000 Praha 2, Katerinská 32, CSSR): Nachweis vonPrajmaliumbitartrat (Neo-Gilurytmal) im Urin durch Dünnschicht-Chromatographie. (Detection ofprajmaliumbitartrate (neo-gilurytmal) in urine by thin-layer chromatography). Pharmazie 42, 550-551 (1987). TLC of theantiarrhythmic prajmalin on silica with methanol - acetic acid - HCl 10:85:5, chloroform - ethanol - acetic acid 15:15:10and toluene - ethanol - acetone - diethyl amine 30:20:20:1. Detection with Dragendorff’s reagent modified by Kaiser andJori (addition of 1 g cersulfat and 1 mol/L, conc. nitric acid). Clinical routine analysis , qualitative identification 32c

61 225 M. OKAMOTO, A. FUTAMURA, SH. GOTO, S. TAMAOKI, (Dep. Pharm., Gifu Prefect. Tajimi Hosp., Tajimi, Japan507): (Determination of glycyrrhizin on 3-amino-propyltriethoxysilane-treated thin-layer chromatographicplates. ) (Japanese). Jap. J. Hosp. Pharm. 13, 11-14 (1987) (Byoin Yakagaku). TLC on amino-bonded silica withbutanol - NH3 - ethanol 5:2:1. Detection under UV. Quantification by densitometry at 254 nm. Pharmaceutical research , quantitative analysis , densitometry , glycyrrhizin 32c

61 226 H. OTSUKA*, Y. HIRAI, T. NAGAO, K. YAMASAKI, (*Inst. of Pharmaceutical Sciences, Hiroshima Univ., School of



61 226 H. OTSUKA*, Y. HIRAI, T. NAGAO, K. YAMASAKI, (*Inst. of Pharmaceutical Sciences, Hiroshima Univ., School ofMedicine, 1-2-3 Kasumi, Hiroshima 734, Japan): Anti-inflammatory activity of benzoxazinoids from roots of CoixLachryma-Jobi var. Ma-yen. Journal of Natural Products 51, 74-79 (1988). TLC of benzoxazinoids on silica withchloroform - methanol - water 15:6:1. Detection under 254 nm UV and by treatment with 10 % sulfuric acid and heating. Pharmaceutical research , agricultural , qualitative identification 32e

61 227 G. PETRI*, A. KERY, E. LEMBERKOVICS, (*Instrumental of Pharmacognosy, Semmelweis Medical Univ., Budapest,Hungary): Instrumental facilities of standardization of complex plant preparations, paramedicaments. (Hungarian). Acta Pharm.Hungarica 57, 119-132 (1987). TLC methods recommended for medicinal plant analysis: (Allseparations on silica) Volatile oils with benzene - ethyl acetate 95:5 or with hexane; spraying with 3% vanillin + 3%sulfuric acid in ethanol. Separation of flavonoids with ethyl acetate - formic acid - acetic acid - water 100:11:11:26,flavone glucosides with ethyl acetate - formic acid - water 10:2:1, flavone aglucones with toluene - ethyl formiate -formic acid 5:4:1; detection under UV after spraying with methanolic aluminium chloride solution. Two-dimensionaltechniques for anthraquinones: ethyl acetate - methanol - water 200:33:27 (first dimension), toluene - ethyl acetate -formic acid 10:8:1 (second), detection under UV 366 nm. Separation of organic acids with chloroform - ethyl acetate -formic acid 3:2:2; detection under UV 366 nm after spraying with 3% FeCl3 in methanol. Pharmaceutical research , qualitative identification 32e, 11a, 15

61 228 B. RENGER, K. WENZ, W. ZIEGENBALG, (Mundipharma GmbH, P.O. Box 1350, D-6250 Limburg, FRG): Instrumental TLC as routine method in pharmaceutical quality control. H. TRAITLER, A. STUDER, R.E.KAISER (eds): Instrumental HPTLC, Institute for Chromatography, Bad Dürkheim, FRG (1987), 331-339. Introduction ofthe use of instrumental TLC and HPTLC as a fast, convenient analytical method for testing pharmaceutical rawmaterials, bulk substances and finished drugs with the results being as accurate as those obtained with manytraditional methods. Instrumental TLC methods have been fully validated. Pharmaceutical research , quantitative analysis , densitometry , review , instrumental TLC , quality control 32a

61 229 T. SCHNEIDER*, W. SIEGMUND, M. ZSCHIESCHE, R. KALLWELLIS, A. SCHERBER, (*Inst. für KlinischePharmakologie der Ernst-Moritz-Arndt-Univ. Greifswald, DDR-2200 Greifswald): Zum qualitativen Nachweis vonMetaboliten des Dihydralazins beim Menschen. (Identification of metabolites of dihydralazine in man.) Pharmazie43, 33-36 (1988). TLC of 2-pyrazole, 3-pyrazole and 4-pyrazole on silica with ethyl acetate - ethanol - cyclohexane -dioxane - 25% NH3 40:20:15:15:10 and chloroform - cyclohexane - diethyl amine 70:20:10. Detection with Dragendorffreagent. Clinical routine analysis , qualitative identification 32c

61 230 D.R. SCHRÖDER*, K. NAKANISHI, (*Dep. of Chemistry, Columbia Univ. N.Y., New York 10027, USA): A simplifiedisolation procedure for azadirachtin. Journal of Natural Products 50, 241- 244 (1987). TLC on silica with chloroform- acetonitrile 3:1. Visualization with 3 % vanillin in ethanol containing 1 % sulfuric acid and gentle heating. Agricultural , qualitative identification 32e

61 231 A. SLAGOWSKA*, I. NOWOSIELSKA, J. GRZYBEK, (*Dep. of Virology, Medical Academy, Czysta 18, 31- 121Kraków, Poland): Inhibition of herpes simplex virus replication by Flos Verbasci infusion. Polish Journal ofPharmacology and Pharmacy 39, 55-61 (1987). TLC analysis of flavonoids, iridoids, phenolic acids and saponins in thelyophilized infusion obtained from flowers of Verbascum thapsiforme Schrad. Numerous separation systems andstaining procedures. Clinical chemistry research , qualitative identification 32e, 8, 14, 21

61 232 M.V. ST-PIERRE, K.S. PANG*, (*Fac. Pharm.& Dep. Pharm., Fac. Med., Univ. Toronto, Toronto, Ontario, Canada): Determination of diazepam and its metabolites by high-performance liquid chromatography and thin-layerchromatography. J. Chromatogr. 421, 291-307 (1987). TLC on silica with chloroform - ethyl acetate - ethanol - NH381:5.5:11:2. Visualization under UV. Quantification of the labelled compounds by liquid scintillation counting. Goodagreement of TLC results with HPLC results. Pharmaceutical research , quantitative analysis , comparison of methods , diazepam , scintillation counting 32b

61 233 P. SURMANN, (Inst. Pharmazie u. Lebensmittelchemie, Univ. Würzburg, Am Hubland, D-8700 Würzburg, F.R.G.): Chromatographische Methoden des DAB 9. (Chromatographic methods of DAB 9). Dtsch. Apoth. Ztg. 128, 14-16(1988). Application of TLC as method for testing identity and purity using reference substances. Examples are TLC ofIndometacin on silica and on HPTLC silica with ether - petrol ether - acetic acid 140:60:0.5. Recommendation ofstandardized plates, HPTLC Linear Developing chamber and standardization of sample application. Qualitative identification 32

61 234 G. SZELECZKY*, L. VERECZKEY, (*Chemical Works of Gedeon Richter Ltd., H-1475 Budapest 10, Hungary):



61 234 G. SZELECZKY*, L. VERECZKEY, (*Chemical Works of Gedeon Richter Ltd., H-1475 Budapest 10, Hungary): Pharmacokinetic and metabolic studies of vinpocetine on dogs. II. Metabolism. Polish Journal of Pharmacologyand Pharmacy 38, 269-275 (1987). TLC of vinpocetine on silica with dichloromethane - methanol 10:1 and 5:1, ethanol- conc. NH3 - water 156:25:19, dichloromethane - ethanol - conc. NH3 5:4:1. Detection under UV. Pharmaceutical research , qualitative identification 32a

61 235 I. VOGEL*, G. ROSTOCK, R. GÜNZEL, H.D. FAULHABER, (*Zentralinst. für Herz-Kreislauf-Forschung, DDR-1115Berlin, Wiltberg Str. 50): DC-Nachweis von Talinolol (Cordanum (R)) im Urin, eine Screeningmethode zurKontrolle des Einnahmeverhaltens bei Hypertonikern. (TLC identification of talinolol (Cordanum (R)) in urine, ascreening method for monitoring administration of this anticonvulsant.) Pharmazie 42, 165-166 (1987). TLC of talinololon silica with ethyl acetate - methanol - NH3 80:10:10. Detection under UV 335 nm. Clinical chemistry research , qualitative identification , therapeutic drug monitoring 32c

61 236 D. WANG (Wang Dunrui), X. LIN (Lin Xingyu), H. ZHANG (Zhang Huizhu, (Dep. Biochem., Shanghai 2nd Med.Univ.,Shanghai, P.R. China): (Thin-layer chromatographic fluorometry of artemisinin and deoxyartemisinin). (Chinese). Acta Pathol.Sinica 8, 355-358 (1987) (Zhongguo Yaoli Xuebao). TLC on silica with sulfuric acid - acetic acidsystem. Determination by fluorometry at 390 nm and 460 nm, resp. for excitation and 430 nm and 500 nm, resp. foremission after heating. Detection limit 60 and 10 ng, resp. Pharmaceutical research , quantitative analysis , densitometry , artemisinin , deoxyartemisinin 32b, 22

61 237 X. WANG (Wang Xuming), Y. ZENG (Zeng Yaowu), (Inst. Drug Instrum. Cont., PLA, P.R. China): (Determination ofetamsylate by fluorescence quenching TLC scanning method). (Chinese). Chinese J. Pharm. Anal. 7, 150-152(1987) (Yaowu Fenxi Zazhi). TLC on silica with ethyl acetate - ethanol 7:3. Quantification by densitometry at 300 nm. Pharmaceutical research , quantitative analysis , densitometry , etamsylate 32c

61 238 W. XIE (Xie Wenlin), (Inst. No. 135, Jilin Provin. Dep. Pub. Secur., Changchun, P.R. China): (Determination ofbarbiturates by thin-layer chromatography. ) (Chinese). Chinese J. Pharm. Anal. 8, 46-47 (1988) (Yaowu FenxiZazhi). TLC of 5 barbiturates on silica with benzene - diethyl amine 8:2. Determination by densitometry at 350 nm. Pharmaceutical research , quantitative analysis , densitometry , barbiturates 32c

61 239 P. XIE, Y. YAN, (Guangzhou Institute for Drug Control, 23 Xicun, Xizeng Road, Guangzhou, Guangdong, P.R. China): Reinvestigation on TLC/HPTLC of Ginseng Part I. Optimization of TLC/HPTLC of ginsenosides. H. TRAITLER, A.STUDER, R.E. KAISER (eds): Instrumental HPTLC, Institute for Chromatography, Bad Dürkheim, FRG (1987),401-413. TLC/HPTLC on silica with chloroform - ethyl acetate - methanol - water 15:40:22:10. Visualization by dippingin 5% sulfuric acid - ethanol. Detection by fluorodensitometry. Discussion of the optimization of chromatographicconditions. Pharmaceutical research , densitometry , ginsenosides 32e, 14

61 240 CH. YE (Ye Chongyi), ZH. WANG (Wang Zhiqiang), (Jiangsu Prov. Inst. Drug Cont., Nanjing, Jiangsu, P.R. China): (Quality control of Schizonepeta tenni folia essential oil from different localities. ) (Chinese). ChineseJ.Pharm.Anal. 8, 33-36 (1988) (Yaowu Fenxi Zazhi). TLC of d-menthone and 1-pulegone on alkali-modified silica withpetrol ether - ethyl acetate 9:1. Detection by spraying with vanillin - sulfuric acid - ethanol 1:1:18 and heating at 80 °Cfor 20 min. Pharmaceutical research , qualitative identification , essential oil 32e, 15b

61 241 L. YU (Yu Linrong), J. LI (Li Jilai), (First Military Med. Col., Guangzhou, P.R. China): (Determination of triptolide inthe common threewingnut, Tripterygium wilfordii, by thin-layer chromatography). (Chinese). J. Chinese HerbMed. 19, 66-68 (1988) (Zhongcaoyao). TLC on silica with chloroform - ether 2:1. Detection by spraying with 2%3,5-dinitrobenzoic acid and 5% NaOH in ethanol. Quantification by densitometry at 218 nm. Recovery > 96%.Calibration curve was linear over range at 0.2-1.0 µg. Pharmaceutical research , quantitative analysis , densitometry , triptolide 32e, 15a

61 242 A. ZEJC*, J. OBNISKA, E. CHOJNACKA-WOJCIK, E. TATARCZYNSKA, B. WICZYNSKA, (*Dep. of PharmaceuticalChemistry, Medical Academy, Skaleczna 10, 31-065 Kraków, Poland): Synthesis and anticonvulsant properties ofN-methyl-pyridyl derivatives of m- and p-bromophenylsuccinimides. Polish Journal of Pharmacology andPharmacy 39, 91-95 (1987). TLC or N-methylpyridyl derivatives of bromophenylsuccinimide on silica with chloroform -acetone 9:1. Visualization with 0.05M iodine in 10% HCL. Pharmaceutical research , qualitative identification 32a, 23d

61 243 B. ZHANG (Zhang Baichong), L. WANG (Wang Lanxia), (Gansu Provin. Inst. Drug Cont., Lanzhou, P.R. China):



61 243 B. ZHANG (Zhang Baichong), L. WANG (Wang Lanxia), (Gansu Provin. Inst. Drug Cont., Lanzhou, P.R. China): (Separation of some herbal medicinal compounds by thin-layer chromatography. ) (Chinese). J. Chinese HerbMed. 19, 14-15 (1988) (Zhongcaoyao). TLC of 6 herbal medicinal compounds on silica and alumina with differentsolvent systems. Detection by spraying with various reagents. Quantification by densitometry. Pharmaceutical research , quantitative analysis , densitometry , preparative TLC , herb medicines 32e

61 244 D. ZHANG (Zhang Dengke), ZH. SHA (Sha Zhenfang), W. SUN (Sun Wenji), (Taibai Med. Mater. Co., Shanxi, P.R.China): (Quantitative determination of arbutin and gallotannin in Pyrola calliantha. ) (Chinese). J. Chinese Trad.Med. 12, 301-302 (1987) (Zhongyao Tongbao). TLC on silica with ethyl acetate - methanol - water 20:3.4:2.7.Quantification by densitometry at 290 nm and 720 nm, resp. Pharmaceutical research , quantitative analysis , densitometry , arbutin , gallotannin 32e

61 245 D. ZHANG (Zhang Diqun), E. ZHANG (Zhang Erqiao), (Hebei Med. Coll., Shijiazhuang, Hebei, P.R. China): (Identification of nifedipine and its analogs by thin-layer chromatography. ) (Chinese). Chinese J. Med. Ind. 18,266-267 (1987) (Yiyao Gongye). TLC of nifedipine and its analogs, nitrendipine and nimodipine on silica with hexane -chloroform - ethyl acetate 2:3:1 or hexane - acetone 1:1. Identification by Rf comparison and UV spectroscopy. Pharmaceutical research , qualitative identification , nifedipine 32c, 4e

61 246 P. ZHU (Zhu Pinyie), Y. BAI (Bai Yungjun), (Guangzhou Inst. Drug Cont., Guangzhou, P.R. China): (Determination ofglibenclamide in Xiaoke pill by thin-layer chromatography/UV spetrophotometry. ) (Chinese). Chinese J. Pharm.Anal. 8, 27-29 (1988) (Yaowu Fenxi Zazhi). TLC on silica with chloroform - ethanol 100:4 or 100:6. Quantification afterelution with chloroform - ethanol 15:1 by UV spectrophotometry at 301 nm. Pharmaceutical research , quantitative analysis , glibenclamide 32c

33. Inorganic substances

61 247 Q. BI (Bi Qiongsi), D. XU (Xu Daozhuang), K. CHEN (Chen Kongguo), (Hunan Univ. Changsha, P.R. China): (Analysis of oxy acid ions of phosphorus (V) by thin-layer chromatography. ) (Chinese). J. China Assoc. Inst.Anal. 6, 22-24 (1987) (Fenxi Ceshi Tongbao). TLC of ortho-, meta- and pyrophosphate ions on silica with ethanol -water - acetic acid - nitric acid 30:10:2:0.05. Elution with water. Quantification by spetrophotometry. Quantitative analysis , oxy acid ions of phosphorus (V) 33b

61 248 G. BOTTURA, M.A. PAVESI, (Ist. Chim. G. Ciamician, Univ. Studi, Bologna, Italy): Thin-layer chromatography ofsome organic selenium compounds. Microchem. J. 35, 223-226 (1987). TLC of selenochrom-4-one,selenochroman-4-ol, 2-nitrobenzylphenylselenide, 2-aminobenzylphenylselenide and 6H-dibenzo[b,d]selenopyran onsilica with chloroform - trichloroethylene 30:70. Detection with conc. sulfuric acid or 4-dimethylaminobenzaldehydesolution. Separation in µg-scale. Qualitative identification 33a

61 249 G. CHENG (Cheng Guifang), ZH. HU (Hu Zhaosheng), X. JIA (Jia Xiping), J. ZHENG (Zheng Jieheng), (East ChinaNormal Univ., Shanghai, P.R. China): (Separation of lanthanide by thin-layer chromatography. ) (Chinese).Chinese J. Chromatogr. 6, 108-110 (1988) (Sepu). TLC on silica with di-(2-ethyl-hexyl)phosphate -2-ethylhexyl(2-ethylhexyl) phosphoric acid - THF - nitric acid - isoproylether in different ratios. Detection by sprayingwith 0.02% ethanolic chlorophosphon-a20-m-NO2 8.1.1994 reagent. Qualitative identification , lanthanide , rare earth 33a

61 250 D.T. HAWORTH, J.K. LUNKENHEIMER, M. DAS, (Dept. Chem., Marquette Univ., Milwaukee, Wisconsin 53233, USA): Normal and reversed phase thin-layer chromatography of transition metal complexes ofmonothio-ß-diketones. J. Liquid Chrom. 10, 1327-1348 (1987). TLC of 82 metal complexes of monothio-ß-diketoneson silica or RP-18 silica with 11 single solvents, 14 binary solvents and 6 reversed phase solvent systems. Detectionby intense color of the complexes and by inspection under UV.

33a

61 251 A. LIU (Liu Anxi), G. Rao (Rao Guichun), Y. WANG (Wang Yingwei), (Xiangtan Univ., Xiangtan, P.R. China): (Separation of rare earth elements by reversed-phase thin-layer chromatography) (Chinese). J. Xiangtan Univ.1, 77-80 (1987) (Xiangtan Daxue Ziran Kexue Xuebao). TLC on primary amine N1923-silica with sulfuric acid. The plotof log (1/Rf-1) against the atom numbers of the rare earths divides the rare earths into 4 groups following the order ofatom number: La-Nd, Pm-Gd, Gd-Ho and Ho-Lu. Rare earths 33a, 2

61 252 A. MOHAMMED, N. FATIMA, (Chem. Section, Z.H. College of Eng.& Technolgy, Aligarh Muslim Univ., Aligarh 202 001,



61 252 A. MOHAMMED, N. FATIMA, (Chem. Section, Z.H. College of Eng.& Technolgy, Aligarh Muslim Univ., Aligarh 202 001,India): TLC separation of microgram quantities of iron(II) from milligram quantities of iron(III). J. Liquid Chrom.10, 1349-1358 (1987). TLC of Fe(II) and Fe(III) salts (nitrate, chlorides, sulfates) on silica with 9 solvent systemscontaining formic acid. Estimation by visual comparison. Qualitative identification 33a

61 253 A. MOHAMMED, N. FATIMA, (Chem. Section, Z.H. College of Eng.& Technolgy, Aligarh Muslim Univ., Aligarh 202 001,India): Cation thin-layer chromatography in mixed organic solvents containing s-butylamine: Separation ofZn(II) from Cd(II) and Cu(II). Chromatographia 23, 653-656 (1987). TLC on silica with mixed organic solventscontaining s-butyl amine. Examination of the separation of Zn(II) from Cd(II) in the presence of certain anions and atvarious pH values of the sample solution. S-butyl amine - acetone - formic acid 2:6:2 was found the best solventsystem. Cation TLC 33a

61 254 B. STEINBRECH, (Inst. f. Anorg. Chem., Johann Wolfgang Goethe Univ., D-6000 Frankfurt/M., F.R.G.): Thin-layerchromatography and high pressure liquid chromatography of metal chelates. J. Liquid Chrom. 10, 1-48 (1987).TLC of dithizonates on e.g. silica with dichloromethane - benzene; of dithio carbamates on e.g. silica with n-hexane -chloroform - diethylamine 20:2:1; of oxinates on silica with n-butanol - acetic acid - oxine 100:20:5 saturated with water;of thiooxinates on silica with dichloromethane - chloroform - benzene or toluene; of thionates on silica with chloroform -THF 90:10; of ß-diketonates on cellulose with ternary solvent mixtures. In addition separations ofmonothio-ß-diketonates, ß-dithioketonates, dialkylbenzoyl-thiourea chelates are reported.

33a

61 255 J. WANG (Wang Jun), ZH. HU (Hu Zhaosheng), (Dep. Chem., East China Normal Univ., Shanghai, P.R. China): (Determination of cerium sub-group and ytterbium sub-group elements by thin- layer chromatography. )(Chinese). Chinese J. Chromatogr. 6, 105-108 (1988) (Sepu). TLC on silica with mono-(2-ethylhexyl)phoshate -iso-propyl ether - ether - nitric acid 1:10:6:1 and 1:10:6:1.05. Detection by spraying with 0.02%chlorophosphonazo-m-NO2 rea gent. Quantification by densitometry at 660 nm. Detection limit 7.4 x 10 to the minus8 g. Quantitative analysis , densitometry , cerium sub-group elements , ytterbium sub-group elements , rare earth 33a

34. Radioactive and other isotopic compounds

61 256 G. KLIVENYI*, G. JANOKI, J. TÖRKO, (*Inst. „Frédéric Joliot-Curie“, H-1775 Budapest, P.O. Box 101, Hungary): Theuse of overpressure thin-layer chromatography in the quality-control of radioactive drugs. (Hungarian).Gyógyszerészet 31, 221-224 (1987). OPTLC of Albumon, Fyton, Pyron of 99m/Tc labelled on silica with acetone. Quality control , qualitative identification 34, 32a

35. Other technical products and complex mixtures

61 257 Q. DENG (Deng Qinying), K. LI (Li Kaozheng), X. SUN (Sun Xiaowei), (Dep. Chem., Zhongshan Univ., Guangzhou,P.R. China): (Quantitative analysis of sucrose fatty acid esters by thin-layer chromatography. ) (Chinese). J.China Assoc. Inst. Anal. 6, 19-22 (1987) (Fenxi Ceshi Tongbao). TLC on silica with chloroform - methanol - acetic acid- water 80:10:8:2. Detection by exposing to iodine vapour. Quantification by densitometry at 520 nm. Quantitative analysis , densitometry , sucrose fatty acid esters 35a, 11a

61 258 T. ERDOSS, (Agricultural Publisher, H-1054 Budapest, Báthori u.10, Hungary): Proceedings of wines. (Hungarian).Mesogazdasági Kiadó (Agricultural Publisher), Budapest 1986, 164-166. TLC identification of the preservativessalicylic-,sorbic- and benzoic acids in wine on silica with petrol ether - dibutyl ether - formic acid - acetic acid 80:35:3:3.Detection under UV 366 nm. Detection limits: 2 mg/L for salicylic acid and for sorbic acid, 10 mg/L for benzoic acid. Agricultural , qualitative identification 35b

61 259 K. ROBARDS*, S. DILLI, (*School of Appl. Sci., Riverina-Murray Inst. of Higher Education, P.O. Box 588, WaggaWagga, New South Wales 2650, Australia): Analytical chemistry of synthetic food antioxidants, a review. Analyst112, 933-943 (1987). A review on TLC of synthetic food antioxidants with more than 20 references. Review , antioxidants 35b

61 260 K. THOMA*, O.E. SCHUBERT, (*Inst. für Pharmazie u. Lebensmittelchemie der Ludwig-Maximillians- Universität



61 260 K. THOMA*, O.E. SCHUBERT, (*Inst. für Pharmazie u. Lebensmittelchemie der Ludwig-Maximillians- UniversitätMünchen, Lehrstuhl für Pharm.Techn., Sophienstrasse 10, D-8000 München 2, F.R.G.): Thiomersal - Wie chemischinstabil ist dieses Konservierungsmittel? (On the chemical stability of the preservative Thiomersal). Dtsch.Apoth. Ztg. 127, 1867-1869 (1987). TLC of thiomersal, thiosalicylic acid, dithiosalicylic acid on silica with chloroform -methanol NH3 (25%) 60:30:10. Visualization with dithizon. Pharmaceutical research , qualitative identification 35b, 24

37. Environmental analysis

61 261 D.L. FOX, (Dep. Environ. Sci. & Eng., Univ. North Carolina at Chapel Hill, Chapel, North Carolina 27514, USA): Airpollution. Anal. Chem. 59, 280R-294R (1987). A review on TLC analysis of polycyclic aromatic hydrocarbons in airpollution. Also other techniques. Review , air pollution , polycyclic aromatic hydrocarbons 37b, 5b

61 262 K. KAMATA*, N. MOTOHASI, (Tokyo Metropolitan Research Lab. of Public Health, 24-1, Hyakunincho 3-chome,Shinjuku-Ku, Tokyo 160, Japan): Separation of methyl-substituted benz[C]acridines by reversed-phasehigh-performance thin-layer chromatography. J. Chromatogr. 396, 437-440 (1987). Reversed-phase TLC onRP-18 silica with 1. methanol, 2. ethanol, 3. acetonitrile, 4. acetone, 5. ethyl acetate, and 6. chloroform. Detectionunder UV 254 nm. (More than 40 solvent systems were tested.) Environmental , qualitative identification , methyl-substituted benz[C]acridines 37b, 23e



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