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CECT 516 Escherichia coli - Colección Española de … · 2013-11-05 · CECT 516 Escherichia coli...

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CECT 516 Escherichia coli (Migula 1895) Castellani and Chalmers 1919 Other culture collections: ATCC 8739; CCRC 11634; CCTM La 2194; CCUG 10979; CIP 53.126; DSM 1576; Gunsalus strain Crookes; IFO 3972; IMET 11121; LMG 8063; NCFB 904; NCIMB 8545; PCM 2561; VTT E-76039; WDCM 00012 History: CECT, 1982 < NCIMB, 1953 < ATCC < I. C. Gunsalus < G. C. Crooks Isolation source: Human faeces Applications: Reference Strain recommended to be used for UNE-CEN ISO/TS 11133, EN ISO 6888 and ISO 15214 Risk group: 2 Culture conditions (medium, incubation temperature, incubation time): 1, 37ºC, 24h CHARACTERIZATION Miniaturized biochemical test API 20E Growth on selective/differential culture media CECT Certified Strains have been selected as typical representatives of species commonly used as reference material after testing that their biochemical profile and their colony appearance on selective/ differential media conform to the expected results. ENDO AGAR Red colonies, red medium, golden sheen TERGITOL AGAR Yellow colonies with yellow zone. Sometimes with rust coloured centre + - + - - - - - + * - + + - + + - + * + - 1 2 4 1 2 4 1 2 4 1 2 4 1 2 4 1 2 4 1 2 4 ONPG ADH LDC ODC CIT H 2 S URE TDA IND VP GEL GLU MAN INO SOR RHA SAC MEL AMY ARA OX 5 0 4 4/5 5 5 2/3 Identification: 94.7-99.9% ID Escherichia coli C E R T I F I E D CERTIFICADA 20121024 TBX (Tryptone Bile X- glucuronide) AGAR Blue-green colonies Page 1 of 2 *This result may vary
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Page 1: CECT 516 Escherichia coli - Colección Española de … · 2013-11-05 · CECT 516 Escherichia coli (Migula 1895) Castellani and Chalmers 1919 Growth on selective/differential culture

CECT 516 Escherichia coli(Migula 1895) Castellani and Chalmers 1919

Other culture collections: ATCC 8739; CCRC 11634; CCTM La 2194; CCUG 10979; CIP 53.126; DSM 1576; Gunsalus strain Crookes; IFO 3972; IMET 11121; LMG 8063; NCFB 904; NCIMB 8545; PCM 2561; VTT E-76039; WDCM 00012

History: CECT, 1982 < NCIMB, 1953 < ATCC < I. C. Gunsalus < G. C. Crooks Isolation source: Human faeces

Applications: Reference Strain recommended to be used for UNE-CEN ISO/TS 11133, EN ISO 6888 and ISO 15214

Risk group: 2Culture conditions (medium, incubation temperature, incubation time): 1, 37ºC, 24h

CHARACTERIZATION

Miniaturized biochemical test API 20E

Growth on selective/differential culture media

CECT Certified Strains have been selected as typical representatives of species commonly used as reference material after testing that their biochemical profile and their colony appearance on selective/ differential media conform to the expected results.

ENDO AGAR

Red colonies, red medium, golden sheen

TERGITOL AGAR

Yellow colonies with yellow zone. Sometimes with rust coloured centre

+ - + - - - - - + * - + + - + + - + * + -

1 2 4 1 2 4 1 2 4 1 2 4 1 2 4 1 2 4 1 2 4

ONPG ADH LDC ODC CIT H2S URE TDA IND VP GEL GLU MAN INO SOR RHA SAC MEL AMY ARA OX

5 0 4 4/5 5 5 2/3

Identification: 94.7-99.9% ID Escherichia coli

C E R T I F I E D

C E R T I F I C A D A

20121024

TBX (Tryptone Bile X-glucuronide) AGARBlue-green colonies

Page 1 of 2

*This result may vary

Page 2: CECT 516 Escherichia coli - Colección Española de … · 2013-11-05 · CECT 516 Escherichia coli (Migula 1895) Castellani and Chalmers 1919 Growth on selective/differential culture

CECT 516 Escherichia coli(Migula 1895) Castellani and Chalmers 1919

Growth on selective/differential culture media

C E R T I F I E D

C E R T I F I C A D A

CTSM (Sorbitol MacConkeyagar with Cefixime Tellurite) AGARPink colonies

VRBL (Violet Red Bile Lactose Agar) AGAR

Red-purple colonies with or without halos

VRBGA (Violet Red Bile Glucose Agar) AGAR

Red-purple colonies surrounded by purple haloes

20121024Page 2 of 2

MYP (Mannitol Egg YolkPolymyxin) AGARGrowth inhibition

Left: Brilliant Green Bile Lactose BrothRight: Laurylsulfate Tryptose BrothBoth: Turbidity with gas production

EC Broth

Turbidity with gas production


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