1. High adhesive property of animal cells 2. Promotion of cell spreading 3. Appropriate for three-dimensional culture
VitrigelⓇ is a registered trademark of National Agriculture and Food Research Organization (NARO).This product is supported by Agri-Health Translational Research Project from the Ministry of Agriculture, Foresty and Fisheries of Japan.
Product No.08363-96
Description 1 plate with lid (12 individual incerts)
Product namead-MED VitrigelⓇ2
Cell Culture Incertad-MED Vitrigelad-MED Vitrigel ® ® 22
High adhesive property of animal cells 2. Promotion of cell spreading
ad-MED Vitrigel® 2 is a upgraded product from ad-MED Vitrigel®
ad-MED Vitrigel® 2 is a upgraded product from ad-MED Vitrigel®
ad-MED VitrigelⓇ 2 is a cell culture insert using a collagen VitrigelⓇ membrane as a scaffold for cell culturing. ad-MED VitrigelⓇ 2 indicates high adhesive property against various kind of animal cells. Also, it is useful for fabrication of three- dimensional tissue models appropriate for drugs and cosmetics development.
Ordering information
1. High adhesive property of animal cellsFeature
Qty. /Pk. 1
High adhesive property of animal cells
ad-MED VitrigelⓇ 2 indicate excellent cell adhesive property as compare to other cell culture inserts using plastic membrane.
ad-MEDVitrigelⓇ 2
ad-MEDVitrigelⓇ
Product A(PET)
Product B(PET)ad-
MED
VitrigelⓇ 2ad-MED
VitrigelⓇ
Product A
(PET) Pro
duct B
(PET)
0
20
40
60
80
100
120(%)
Cell adhesion rate
500 µm
Promotion of cell spreading
ad-MED VitrigelⓇ 2 is promotes rapid cell spreading and forming a focal adhesion.
blue: nucleired: Vinculingreen: Actin20 µm
Incubation time1h 5h1h 5h
ad-MED VitrigelⓇ 2 ad-MED VitrigelⓇ
Cell adhesion rate after 1 hour incubation (cell: HepG2)
Cell morphology (cell: Caco-2)
Appopriate for constructing co-culture systems
Method
ad-MED VitrigelⓇ 2 allow to co-culture different type of cells on both inner and outer side of collagen Vitrigel membrane by using option ring.
Purpose ・Analysis of paracrine effects between two different types of cells ・Fabrication of tissue models using two different types of cells
Option ring
①Set a option ring to ad-MED VitrigelⓇ 2
②Seed cells to back side
③Remove a option ring and set ad-MED VitrigelⓇ 2 in a multi-well plate
【 Example of use ~ Co-culture of PC-12 and L929 cells ~】Co-culture of PC-12 and L929 cells using a option ring promotes neurite outgrowth of PC-12 cells compared with ordinary co-culture.
0
10
20
30
40
1Co-culture using optionring
Ordinaryco-cultureblue: nuclei red: Neurofilament-L (PC-12)
green: Actin (L929)
Co-culture using option ring 20 µm
Common co-culture
PC-12
PC-12
L929
L929
Left: immunostaining, Right: Comparison of neurite length
neurite length (µm)
Optimize culture systems for fabricating tissue modelsad-MED VitrigelⓇ 2 allows to conduct a variety of cell culture systems in vitro represent environments for cells in vivo.
Product lineup
ReferencesTakezawa T. et al. Cell Transplant. 13: 463-473, 2004.Takezawa T. et al. Toxicol In Vitro. 25: 1237-1241, 2011.Takezawa T. Journal of Biosciences and Bioengineering. 91: 214-217, 2013Yamaguchi H. et al. Toxicol Sci. 135: 347-355, 2013.
Dec. 2016
Liquid phaseculture
Air-liquid interface culture Common co-culture
Co-culture using option ring
※ Please contact us whenever you have questions.
12 well
24 well
96well
ProductName
Number of well 12 well 12 well 24 well 96 well
Aria ofmembrane 1.0 cm2 1.0 cm2 0.33 cm2 0.11 cm2
Quantity ofmembrane
CollagenVitrigel®
Package 12 wells / plate
-
CollagenVitrigel®
CollagenVitrigel®
CollagenVitrigel®
ProductNo.
08360-96 08363-96 08364-96
ad-MEDVitrigel®
ad-MEDVitrigel® 2(12 well)
ad-MEDVitrigel® 2(24 well)
ad-MEDVitrigel® 2(96 well)
KANTO CHEMICAL CO., INC.REAGENT DIVISION
East Muromachi Mitsui BLDG, 2-1, Nihonbashi Muromachi 2-chome, Chuo-ku, Tokyo, 103-0022, JAPAN
+81-3-6214-1092 +81-3-3241-1053
TelephoneTelefax
http://www.kanto.co.jp E-mail; [email protected]
12 wells / plate
24 wells / plate
96 wells / plate