Yu-Guang ZHOU
China General Microbiological Culture Collection Center
Institute of Microbiology, Chinese Academy of Sciences
Beijing, 2016-09-07
CGMCC: Promoting the Utilization
of Microbial Resources
2.
3.
4.
1. About CGMCC
Quality Management System
Deposit Biological Material for Paten Purpose
Research on Microbial Diversity
Contents
the core and largest service culture collection in China, a nonprofit
organization financed by the CAS.
1979, CGMCC in the IM-CAS registered as a member of the China Committee
for Culture Collection of Microorganisms (CCCCM)
1985, the Chinese Patent Office recognized CGMCC as the local depositary
of microorganisms for patent purposes in China.
1995, CGMCC has acquired the status of International Depositary Authority
(IDA) under the Budapest Treaty
1996, CGMCC has registered as a member of the Committee for Type
Culture Collection of CAS
2003, became a member of the Platform of National Science & Technology
Infrastructure
About CGMCC
Collect
/Deposit
Distribute
Identify
Database Patent
Research
Preserve
Roles of
CGMCC
Functions of CGMCC
collection, preservation, distribution microbial
strains as national public infrastructure of
biological resource center.
Development of core technologies for, and of
value creation from microbial resources.
contribution to scientific community
by serving high quality microbial
resources and ensuring their
sustainable use, to promote and
sustain the biotechnology innovation.
Industrial utilization
Environmental utilization
Agricultural utilization
Health utilization
Biodiversity of the collection (2015)
Annually ca. 2000 new strains are deposited to
CGMCC public open collection including type strains,
reference strains, and strains relevant to human health,
environment, industry and agriculture.
Genera Species Strains
Bacteria 849 1908 12600
Actinomycetes 155 1264 7220
Yeasts 160 667 5360
F. fungi 658 1851 16750
Patent deposit 11977
Total 1822 5690 53907
Distribution:
Preservation:
- general collections
- safe depository
- patent depository
Identification & Contract test:
- Identification by polyphasic approaches
- Special analysis: G+C content analysis,
DNA-DNA hybridization, Cell wall, MK,
Fatty acid, ……
- anti-microorganisms testing
- ……
Training and consulting
CGMCC, as a Service Provider
Provide users with the products
and services they require.
Products and services should
be of consistently high quality
and fulfill product claims in the
catalogue.
CGMCC, A Good Partner of
Your Research, Enterprises
Quality Control: building
confidence between culture
collection and its users
Since 2008, according to OECD Best Practice Guidelines
for BRCs and WFCC Guidelines, China General
Microorganism Culture Collection (CGMCC) was
starting to develop its quality management system (QMS);
The QMS of CGMCC is also compliant with ISO 9001
requirements so as to achieve an international level of
quality.
In 2010, ISO 9001:2008 certification together with
certifications of ISO 14001 and GB/T 28001 in CGMCC
had been audited and issued by CQC.
In 2016, ISO 9001:2015 certification.
Activities on QMS in CGMCC
Scope of CGMCC QMS Certification
Users Characterization
of
Microorganisms
Strains
Genes
Collection Identification Storage Supply
Organisms
DNA clones
NP
Data
Da
tab
as
e
Quality Control
Depositors
Collection, preservation of biological materials, including safe
depository and patent-related biological materials depository
Distribution of the preserved biological materials
Contract microbial identifications and tests.
Quality Control of Depositions
Viability & stability
Revive preserved specimens
Accelerated storage test after long years
Purity
Macro-and microscopic observations
Picture of colony and cell shapes
Identity
16S rDNA sequencing
ITS & D1/D2 sequencing
over 10000 CGMCC strains’ quality tests
have been finished during last 4 years.
Preservation & Maintenance
Safe backup duplicate stocks (another site)
object：each strain preserved in CGMCC
method：freezing (LN)
number：2 ampoules
Master duplicate stocks
object： each strain preserved in CGMCC
methods：freezing (LN) and freeze-drying、paraffin
sealing slant (if necessary)
number：5 ampoules (freezing), 10 ampoules (freeze-dry)
Distribution stocks
object：strains of catalogue
methods：freeze-drying or freezing
Number：10 ampoules/more (freeze-drying), 5 ampoules
/more (freezing)
How does the culture collection check
the acquisition of the microbe is
legitimate or not under NP
How dose the culture collection
support the easy using for scientific
research under NP
How dose the culture collection
support the benefit sharing under NP
1992，CBD Contracting Party
2016-09-06，NP Contracting Party
CGMCC accepts four kinds of deposition
General deposit.
Deposit with restrictive conditions, general deposit with
restrictive conditions by depositors when they deposit their
strains in the CGMCC. For example, just can be used for
scientific purpose.
Safe deposit, a service for the long-term preservation of
microorganisms. CGMCC can not open them to publics
Patent deposit, for patent purpose according to Budapest
Treaty.
General Deposit at CGMCC
For public open collection and no accession fee.
Microbial material deposited in this manner will be published in
a CGMCC catalog/on-line database and available to the third
party in both academic and industrial/agricultural institutions.
Depositors must state the origin country of strains or transferred
history. The microbial strains will be not accepted without these
information.
The general MAA has been published to state the rights of
depositors and CGMCC (agreement on deposition).
Distribution at CGMCC
CGMCC just provide the strains to the registered users.
CGMCC signs the MTA with users when they register as CGMCC users.
Through the MTA, the users will be informed their duties and rights, like:
- can not transfer the CGMCC strains to third party except they get the
permission from CGMCC.
- should understand the benefit sharing with origin depositors when they
use CGMCC strains for commercial purpose.
According to asking from the depositors, CGMCC will inform them who
used their strains deposited in the CGMCC
1985, the Chinese Patent Office recognized CGMCC as the local depositary
of microorganisms for patent purposes in China.
1995, CGMCC has acquired the status of International Depositary
Authority (IDA) under the Budapest Treaty
The Budapest System provides a practical business solution in the
international patent process when the inventions involving microorganisms.
Budapest Treaty Contracting Parties: 80 countries
International Depositary Authority (IDA): 45 culture collections (04/01/2016)
Microorganisms for Patent Purpose under BT
Patent Microorganisms Deposited in CGMCC
By the end of 2015: 11977
TOP 2 among 45 IDAs
under Budapest Treaty
Annually ca. 1500 new
biological materials are
deposited to CGMCC patent
collection including microbial
strains, viruses, microalgae,
cell lines, plant seed etc.
CGMCC-CN
IPOD-JP
CGMCC
2015, the new regulation on Biological Material for Patent Purpose was
published by State Intellectual Property Office
All the contents are similar with before except one, about deposits after
30/35 years.
According to regulation, if the depositors do not contact with the culture
collections to make arrangement for their biological material during one
year after storage period of 30/35 years, the culture collection will get the
authority to handle these biological material
Storage fees decreased from USD 800 to RMB 3500 (≈USD 500)
Storage period of 30 year: Rule 9 of regulation under Budapest Treaty
Deposits after 30 (plus 5 ) years
Taxonomic Studies
Recently, CGMCC mainly interested on
the research of psychrophilic and
psychrotolerant bacteria isolated from
glaciers and plateau lakes; marine
bacteria isolated from ocean; and
endophyte isolated from desert plant.
Thousands of strains have been
isolated, identified and deposited.
Novel taxon described by CGMCC researchers
• Idiomarina indica, 2013
• Flavobacterium marinum, 2013
• Ornithinimicrobium tianjinense, 2013
• Dyadobacter tibetensis, 2013
• Mycetocola zhadangensis, 2013
• Massilia yuzhufengensis, 2013
• Glaciihabitans tibetensis, 2014
• Flavobacterium lacus,2014
• Arcticibacter pallidicorallinus, 2014
• Colwellia aquaemaris, 2014
• Roseibium aquae, 2014
• Rheinheimera tuosuensis 2014
• Wenyingzhuangia marina , 2014
• Photobacterium aquae, 2014
• Salinibacillus xinjiangensis, 2014
• Frigidibacter albus , 2015
• Hafnia psychrotolerans , 2015
• Arcticibacter eurypsychrophilus , 2015
More new species are being described
• Flavobacterium xueshanense, 2012
• Flavobacterium urumqiense, 2012
• Cryobacterium flavum, 2012
• Cryobacterium luteum, 2012
• Candidimonas bauzanensis, 2012
• Bradyrhizobium huanghuaihaiense, 2012
• Halobellus limi, 2012
• Halobellus salinus, 2012
• Gracilibacillus kekensis, 2012
• Devosia psychrophila, 2012
• Devosia glacialis, 2012
• Nocardioides alpinus, 2012
• Arthrobacter cryoconiti, 2012
• Cryobacterium levicorallinum, 2013
• Nocardioides szechwanensis, 2013
• Nocardioides psychrotolerans, 2013
• Mycetocola miduiensis, 2013
• Flavobacterium noncentrifugens, 2013
1
2
3
Project 1: Microbial Resources of
Daxing'an & Xiaoxing’an
Mountains range
•Coordinator: IM-CAS
• 4 collaborators: IAE-CAS, Beijing Forestry
University, Jilin Agricultural University,
Northeast Forestry University
• Project Period: 2013-2017
Project 2: Microbial Resources in Karst
range in china 's south-west
•Coordinator: IM-CAS
• 6 collaborators: Guizhou Academy of Sciences,
Guizhou Agricultural Academy of Sciences, Guizhou
University, Guangxi University, Guangdong
Institute of Microbiology
•Project Period: 2014-2017
Project 3: Extremophilic
Microbial Resources in Tibetan
plateau
•Coordinator: IM-CAS
• 7 Collaborators: ITPR-CAS, Yunnan
University, ISS-CAS, IGL-CAS, China
University of Geosciences, NIPB-CAS,
Tibet Institute of Plateau Biology
•Project Period: 2015-2018
Projects on Microbial Resource Investigation
supported by MOST
Project 3: Extremophilic Microbial
Resources in Tibetan plateau
Major Objectives:
general understanding the microbial diversity of different environment
in Tibetan plateau (Prokaryotes)
Collecting over 10000 extremophilic microbial cultures (identification
and characterization)
Sharing cultures and data with public
Psychrophiles in glacier Thermophiles in hot spring Halophiles in salt lake
Try to find the potential uses of isolated cultures and contribute
to R&D of valuable products
cellulase, xylanase, esterase, pectinase, lipase, amylase, ……
cold-adapted , heat-stable, acid-resistant,……
Screening of enzymes
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Strain/cell
bank
Mol Mark
database
Metabolite
bank
Functional
gene bank
Enzyme-
strain bank
Gene expression
Mol. finger-printing
High-throughput screening
Identification
THANKS FOR YOUR
ATTENTION
