CHANGE IN ACID ERITHROGRAM
OF LABORATORY ANIMALS IN
CONSTANT ELECTRIC AND
MAGNETIC FIELD
Anna V. Novikova, Anna A. Oleshkevich,
Viktor E. Novikov
Skryabin Moscow State Academy of Veterinary Medicine and Biotechnology
(Moscow SAVMB), Moscow, Russia
Section: Biophysics, Medical Physics
SIX INTERNATIONAL CONFERENCE ON RADIATION AND APPLICATIONS IN VARIOUS FIELDS OF RESEARCH
12/06/ - 16/06/ 2018 | Slovenska Plaza | Budva | Montenegro
RAD 2018
AUTOHEMOTHERAPY
PENDULUM TYPE
EMR
effect of a constant electric and magnetic
field on the suspension of red blood cells
of laboratory rats
+
-
8.3102
V/м
70 Э (5.5 102А/м)
photoelectric turbidimeter
1- photometer- turbidi-meter; 2- cuvette`s thermostatic water-jacket; 3- cuvette with suspension of erythro-cytes; 4- mixer; 5-fitting for fluid supply; 6 - optical sensor block; 7 - analog-digital converter; 8 – recorder (PC+ software) ; 9 –light.
0
0,2
0,4
0,6
0,8
1
0 500 1000
time, s
ch
are
of
lys
is c
ells
ACIDERITHROGRAM (cumulative)ACIDERITHROGRAM (differential)
0
0,0005
0,001
0,0015
0,002
0,0025
0 200 400 600 800 1000
time, s
[ch
are
cells]/
[s]
• The resulting distribution of
erythrocytes by acid resistance
(histogram) was further analyzed by
mathematical modeling. It was
carried out by the histogram
decomposing into the sum of 7
normal distributionsseries. To each
private distribution corresponds one
age group of erythrocytes.
)](exp[7
1
)(32
21 jj
jji
KtKKj
j
Ktdt
dn
t – the time from preface hemolytic ;
(К1, К2, К3) – characteristics distribution .
К1 – partial coefficient one group erythrocytes (the parts of total volume);
К2 – conform to dispersion value;
К3 – the size of time attainment maximum speed hemolysis one group erythrocytes.
0
0,0002
0,0004
0,0006
0,0008
0,001
0 400 800
time, s
N -1
(d
n/d
t) K1=(SDn/N)
K3
K2
mathematical modeling by the histogram decomposing
into the sum of 7 normal distributionsseries
0
0,0005
0,001
0,0015
0,002
0,0025
0,003
0 200 400 600 800 1000
p1(t) p2(t) p3(t)
p4(t) p5(t) p6(t)
p7(t) SUM(t)
0
0,2
0,4
0,6
0,8
1
0 500 1000
experiment model
The simulation erithrogram one
Method reduce to automatic
selection coefficient`s signifi-
cance К1 – К3 (soft method ).
The coincidence simulation curve
and experimental
curve to hallmark adequacy
(to within width grass).
• The change of diffrential ACID ERITHROGRAM after coercion CONSTANT ELECTRIC FIELD
differential acid
erithrogram (control)
0
0,0005
0,001
0,0015
0,002
0,0025
0,003
0 500 1000
time, s
[pa
rt o
f ly
sis
RB
C /
с]
differential acid erithrogram ( 5
min influence ESF)
0
0,0005
0,001
0,0015
0,002
0,0025
0,003
0,0035
0 500 1000
time, s[pa
rt o
f ly
sis
RB
C /
с]
differential acid erithrogram (10
min influence ESF)
0
0,0005
0,001
0,0015
0,002
0,0025
0,003
0,0035
0 500 1000
time, s[pa
rt o
f ly
sis
RB
C /
с]
differential acid erithrogram (15
min influence ESF)
0
0,0005
0,001
0,0015
0,002
0,0025
0,003
0,0035
0 500 1000
time, s[pa
rt o
f ly
sis
RB
C /
с]
• The change of diffrential ACID ERITHROGRAM after coercion CONSTANT MAGNETIC FIELD
differential acid erithrogram
(control)
0
0,0005
0,001
0,0015
0,002
0,0025
0,003
0 500 1000
time, s[pa
rt o
f ly
sis
RB
C /
с]
differential acid erithrogram (5
min influence CMFl)
0
0,0005
0,001
0,0015
0,002
0,0025
0,003
0 500 1000
time, s[pa
rt o
f ly
sis
RB
C /
с]
differential acid erithrogram (10
min influence CMFl)
0
0,0005
0,001
0,0015
0,002
0,0025
0,003
0,0035
0 500 1000
time, s[pa
rt o
f ly
sis
RB
C /
с]
differential acid erithrogram (15
min influence CMFl)
0
0,0005
0,001
0,0015
0,002
0,0025
0,003
0,0035
0,004
0,0045
0,005
0 500 1000
time, s[pa
rt o
f ly
sis
RB
C /
с]
• The change of integral (experimental curve normalized to unit) ACID ERITHROGRAM after coercion CONSTANT MAGNETIC FIELD
integral acid erirhrogram
(control)
0
0,2
0,4
0,6
0,8
1
0 500 1000
time, s
[pa
rt l
ysi
s R
BC
]
integral acid erirhrogram (5 min
influence CMF)
0
0,1
0,2
0,3
0,4
0,5
0,6
0,7
0,8
0,9
1
0 200 400 600 800 1000
time, s
[pa
rt l
ysi
s R
BC
]
integral acid erirhrogram (10 min
influence CMF)
0
0,2
0,4
0,6
0,8
1
0 200 400 600 800 1000
time, s
[pa
rt
lysi
s R
BC
]
integral acid erirhrogram (15 min
influence CMF)
0
0,1
0,2
0,3
0,4
0,5
0,6
0,7
0,8
0,9
1
0 200 400 600 800 1000
time, s
[pa
rt
lysi
s R
BC
]
