1 January 12, 2013 CHANGES TO THE BID DOCUMENT IN PURSUANCE TO THE PRE BID CONFERENCE ON JAN. 10, 2013 In continuation to CCMB Open Tender Document No. CCMB/PUR/EQPT./2012-13 dated 31/12/2012 in newspapers and hosted on CCMB website, a pre-bid meeting took place with interested bidders on January 10, 2013 in the Committee Room of the Director’s Office. Representatives from several firms had participated in this meeting. After thorough discussions, the specifications of many items, as detailed below were modified. The following table details the points of technical specifications modified in view of the discussions held/ requests received or any other clarification provided. Tender Item No. Item Name Changes to Specifications / Clarifications 1 Isolation of Mutations in Rice Genes Using TILLING (Technical Service) Please refer to the revised specifications attached with this document. 2 Fluorescence Microscope - 1 7 Phosphor Imager with Accessories-1 8 Upright Fluorescence Microscope-2 9 Advanced Motorized Inverted Microscope-1 10 Spectral Confocal Microscope-1 14 High Speed Centrifuges-2 15 Floor Model Ultra Centrifuge-1 17 Zebra Fish Imaging & Tracking System-1 18 High Content Screening System-1 19 High Throughput SNP Genotyping and Gene Expression System-1 13 High Throughput Real Time PCR Systems-3+2 = 5 Please refer to the revised specifications attached with this document. EMD amount for each System is Rs.40,000/-. Firm quoting for all 05 machines has to deposit Rs.2,00,000/- EMD 3 SITC of Walk- in-Freezer - 1 SPECIFICATION SHEET OF THE ORIGINAL TENDER DOCUMENT MODIFIED TO THE FOLLOWING EXTENT: 1. Line 4 in ‘Following accessories include while quoting: Existing specs: While quoting for condensing units, quote separately for Reciprocating and Scroll compressors Modified specs: While quoting for condensing units, quote separately for either Reciprocating or Scroll compressors Additions Add the following at the end (line 7) in ‘Following accessories include while quoting’: ‘Provision should be made for an emergency exit’. 5 High Resolution FT-IR System-1 SPECIFICATION SHEET OF THE ORIGINAL TENDER DOCUMENT MODIFIED TO THE FOLLOWING EXTENT: (Pt. 11) Existing specs: Temperature range : 8 deg to 80 deg (programmable/automation) Modified specs: Temperature range : 8 deg to 80 deg (programmable/automation) for liquid samples Contd…
Transcript
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January 12, 2013
CHANGES TO THE BID DOCUMENT IN PURSUANCE TO THE PRE BID CONFERENCE ON JAN. 10, 2013 In continuation to CCMB Open Tender Document No. CCMB/PUR/EQPT./2012-13 dated 31/12/2012 in
newspapers and hosted on CCMB website, a pre-bid meeting took place with interested bidders on January 10, 2013 in the Committee Room of the Director’s Office. Representatives from several firms had participated in this meeting. After thorough discussions, the specifications of many items, as detailed below were modified.
The following table details the points of technical specifications modified in view of the discussions held/
requests received or any other clarification provided.
Tender Item No.
Item Name Changes to Specifications / Clarifications
1 Isolation of Mutations in Rice Genes Using TILLING (Technical Service)
Please refer to the revised specifications attached with this document.
2 Fluorescence Microscope - 1
7 Phosphor Imager with Accessories-1
8 Upright Fluorescence Microscope-2
9 Advanced Motorized Inverted Microscope-1
10 Spectral Confocal Microscope-1
14 High Speed Centrifuges-2
15 Floor Model Ultra Centrifuge-1
17 Zebra Fish Imaging & Tracking System-1
18 High Content Screening System-1
19 High Throughput SNP Genotyping and Gene Expression System-1
13 High Throughput Real Time PCR Systems-3+2 = 5
Please refer to the revised specifications attached with this document.
EMD amount for each System is Rs.40,000/-. Firm quoting for all 05 machines has to deposit Rs.2,00,000/- EMD
3 SITC of Walk- in-Freezer - 1 SPECIFICATION SHEET OF THE ORIGINAL TENDER DOCUMENT MODIFIED TO THE FOLLOWING EXTENT: 1. Line 4 in ‘Following accessories include while quoting:
Existing specs: While quoting for condensing units, quote separately for Reciprocating and Scroll compressors Modified specs: While quoting for condensing units, quote separately for either Reciprocating or Scroll compressors
Additions Add the following at the end (line 7) in ‘Following accessories include while quoting’: ‘Provision should be made for an emergency exit’.
5 High Resolution FT-IR System-1 SPECIFICATION SHEET OF THE ORIGINAL TENDER DOCUMENT MODIFIED TO THE FOLLOWING EXTENT: (Pt. 11) Existing specs: Temperature range : 8 deg to 80 deg (programmable/automation) Modified specs: Temperature range : 8 deg to 80 deg (programmable/automation) for liquid samples
Contd…
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Tender Item No.
Item Name Changes to Specifications / Clarifications
6 Protein Purification Systems-4
NO CHANGE. SPECIFICATION SHEET OF ORIGINAL TENDER DOCUMENT SHALL
BE APPLICABLE
11 High Resolution Mass Spectrometer System-1
12 High Sensitivity CD Spectropolarimeter System-1
16 Arabidopsis Plant Growth Chamber-1
4 Liquid Nitrogen Plant and Accessories - 1
The purchase for this item has been dropped for the time being. Therefore NO tenders are invited for this item.
THE TERMS ‘NON-RESPONSIVE’ , ‘UNRESPONSIVE’, AND ‘UN-RESPONSIVE’ EXISTING UNDER ANY PARA / CLAUSE IN THE MAIN TENDER DOCUMENT ARE TO BE UNDERSTOOD / INTERPRETED AS SAME FOR THE PURPOSE OF SUMMARY REJECTION. PLEASE READ TENDER CONDITIONS CAREFULLY BEFORE SUBMITTING YOUR BID. THE ORIGINAL TENDER DOCUMENT MAY BE DEEMED MODIFIED TO THE EXTENT MENTIONED IN THIS DOCUMENT. INTENDING BIDDERS MUST QUOTE TAKING THE ABOVE CHANGES INTO ACCOUNT FAILING WHICH THEIR BID SHALL BE REJECTED AT THE TECHNICAL STAGE ITSELF.
Sd/-
Stores & Purchase Officer
Centre for Cellular and Molecular Biology Hyderabad
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ITEM NO.1 – ISOLATION OF MUTATIONS IN RICE GENES USING TILLING (TECHNICAL SERVICE) [FILE REF: 34/CCMB/APP/417/P12] Purpose: Use TILLING to isolate mutations in rice genes. Specifications: 1. 8-10 mutations are to be isolated/gene. 2. A total of 12 rice genes that are indicated by CCMB are to be used in this analysis. 3. The size of the genes that are to be TILLED vary between 1.0 kb to 4.0 kb with mutations to be isolated
in exonic regions and upstream regulatory regions, as specified by CCMB. 4. The mutant population of 10,000 M2 families of rice plants shall be provided by CCMB in the form of
~15-20 seeds/family. The seeds are to be germinated and the population is to be grown and maintained in India only.
5. TILLING is to be done in India only. 6. Details of the methodology that is to be used in TILLING using this population are to be provided
including the scheme for pooling and gridding. 7. The time frame for DNA isolation and identification of mutations by TILLING is to be provided. 8. Deliverables:
a. DNA samples of each of the 10,000 M2 families. DNA is required in dried form in a 96 well format. b. 8-10 mutations/gene. The exact sequence changes as compared to the wild type sequence should be indicated.
Essential Qualifications: Established expertise in growing large mutagenized populations of plants, isolation of DNA from plants on a large scale, pooling the DNA in grids and using TILLING for allele discovery in crop plants with evidence of the same. Price details in the Price Bid are to be submitted as explained below. If the price bid is not submitted in this format, the quotation shall be treated as unresponsive and rejected summarily. IMPORTANT:
Bids must be submitted in TWO PARTS (TECHNICAL & PRICE BIDS)
Some of the provisions of this tender document which are applicable in case of supply, installation, and
commissioning of equipments MAY NOT apply in the case of providing of Technical Services. All such
information may be marked as Not Applicable /NA in your bid papers.
Price details in the Price Bid are to be submitted as explained below. If the price bid is not submitted in
this format, the quotation shall be treated as unresponsive and rejected summarily.
(PRICES FOR BOTH THE OPTIONS GIVEN BELOW MUST BE QUOTED IN THE PRICE BID PART)
Option1: Price for the service has to be quoted by inclusive of all services and materials like reagents,
including for DNA isolation, are procured by the service provider.
Option2: Price for the service if CCMB procures all of the required reagents for DNA isolation and supplies
them to service provider. In this option, bidder has to attach a list of all of the required reagents /
chemicals etc. for DNA isolation and supplies them to service provider. The list of reagents, source of
reagents (including catalogue numbers) and quantity of reagents that are required needs to be included in
the quote.
UNSECURED ADVANCE PAYMENT SHALL NOT BE MADE. PAYMENTS CAN BE MADE BASED ON PROJECT
MILESTONES TO BE MUTUALLY DISCUSSED AND DECIDED WITH THE SUCCESSFUL BIDDER.
Complete system should carry one year onsite comprehensive warranty.
The computer should carry three years onsite comprehensive warranty.
Complete Service and technical manuals with detailed documentation, user manual should be supplied along with the system.
List of users of similar research institutions in India to be provided with addresses and contact details.
Technical presentation & demonstration of similar system in other research institutes / centers / universities should be arranged on request.
Criteria for selection would be based not only on specifications, features, clauses and conditions but on proven performance of Upright Fluorescence Microscope and technical support. Therefore all the points mentioned in the tender should be answered.
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ITEM NO. 7 – PHOSPHOR IMAGER ALONG WITH ACCESSORIES, QTY: 1 NO. (FILE REF: 34/CCMB/APP/404/P12)
1) Phosphor Imager: Radioisotopes detectable :
14C,
32P,
33P,
35S,
3H etc.
IP Scanning area for a single sample : 20cm X 40cm Built-in Laser excitation source : ~ 635nm Red Laser light source Emission filter : IP filter Emission detector : PMT Pixel size : 50, 100, 200µm etc. selectable Dynamic range : 4 - 5 orders of magnitude Bit depth : 16 Bit Typical scanning time for 20cm X 40cm : around 2 to 8 minutes 1 System
IP area at 50µm Scanning
Computer connectivity : USB Software : Required software for scanner control, image acquisition and analysis Operating voltage : 230 Volts, 50Hz A.C Warranty : 3 Years comprehensive on-site
2) Imaging plate eraser 1No. 3) Latest Desktop branded PC (Dell / HP) suitable for the above system with 1No.
6GB RAM, 2 X 500GB HDD, DVD Writer, 19” LCD Monitor, Mouse, Keyboard, Network interface, Windows XP / Windows 7 OS etc.
4) HP Laserjet Printer A4 Size, Black & White, 30 ppm and above 1 No. Optional Items:
1) Set of spare Imaging plates and cassettes 20cm X 25cm size 5 No’s 2) Set of spare Imaging plates and cassettes 20cm X 40cm size 5 No’s
Important clauses: 1. The computer offered along with the system should be the latest model compatible with the software offered.
Required interface cards, cables etc to be included. The PC configuration and make to be clearly specified and should include a three year comprehensive onsite global warranty (to be transferred to CCMB for better follow-up in case of failures of PC during & after warranty). It should be from well-known reputed manufacturers only.
2. Detailed literatures (all originals) with technical specifications and features for main systems and all the accessories to be sent along with the offer.
3. Detailed instructions and service manuals with circuit diagrams and all engineering details for the entire system should be supplied with the unit. Commitment to this clause to be made in the offer.
4. Software supplied should be the latest version and any up-gradation in the software to be given free of cost for three years.
5. All standard accessories that would be supplied with the unit to be clearly mentioned in the offer. Other required accessories may be offered separately.
6. Essential spares to be offered separately. 7. The principals / local agents are responsible for the installation and testing of the main System and accessories.
Integration of the main system with the accessories, training of the users etc should be carried out at CCMB immediately after receipt of the system.
8. Complete installation and testing the main system and the accessories to their specifications with standard samples and user samples to be done at the site of installation free of cost. All utility requirements for installation and running the system should be mentioned in the offer.
9. Please indicate the year in which the model offered was introduced into the market and confirm whether the spares for the system would be available for a minimum period of 10 years.
10. Availability of local service support and response time for a service call curing and after warranty to be specified. 11. List of users of the similar models as the one offered, along with the addresses, telephone numbers and e-mail ID’s to
be enclosed with the offer in a separate annexure. 12. Demonstration of similar models as the one offered to be given on request at other customer sites. 13. Technical presentations should be made on request from CCMB. 14. Please indicate the number of installations of the similar models as the one offered, in India. 15. All other clauses of payment details, delivery schedule, shipment, and warranty to be mentioned clearly. Selection of the system would be based on the response to all the above points apart from the technical specifications, features and suitability to CCMB’s requirements and therefore please respond to all the points.
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ITEM NO.8 – UPRIGHT FLUORESCENCE MICROSCOPE WITH CCD DIGITAL CAMERA, QTY: 2 NOS. (FILE REF: 34/CCMB/APP/405/P12) Microscope: 1. Mechanically rugged and sturdy Binocular Upright Microscope with an additional camera port on top of binocular head with a beam splitter for Light path selection Binocular : camera, 100:0, 30:70/50:50/80:20,0:100.. 2. The microscope should have the necessary components or accessories required for DIC/Fluorescence/ Bright field applications. 3. There should not be any pixel shift while acquiring DIC and Fluorescence imaging, both the DIC and Fluorescence images should exactly overlap. 4. Fully apochromatic corrected optical path for fluorescence. 5. Z focus drive with coarse and fine mechanisms on both sides. 6. Objective nose piece with 6 positions. 7. Neutral density filters for both bright field and Fluorescence. 8. Blue and green filters for transmission light path 9. X-Y stage with right hand movement control 10. 12v -100w Halogen lamp with intensity control for transmission light. 11. Objectives Plan Apochromat 10x, 20x, 40x, 60x/63x, 100x with high N.A. for Fluorescence and DIC applications with parfocality. 12. Condenser for bright filed, Dark field, Phase and DIC application with N.A. 0.9 or more 13. Eye piece 10x/22 or more adjustable. 14. DIC accessories for 10x, 20x, 40x, 60x/63x,100x objectives 15. Built in shutters for fluorescence light and transmitted light. 16. Variable field and aperture diaphragms for Bright and Fl applications. 17. 5 Position or more Fl filter turret. 18. Fluorescence filters for DAPI, GFP (narrow band), FITC, CY3, CY5. 19.120W/130W Metal halide lamp source with 1500 hours or more life time for Fluorescence applications with intensity control. 20. The system and software should be user friendly and more flexible for multi user environment. 21. All the above mentioned features (like apochromatic fluorescence path, DIC & Fl without pixel shift) should be supported by literatures/brochures/other evidences. High end Digital camera: 1. High end cooled CCD Colour camera with 1.4 Mega Pixel basic resolution. 2. Pixel size of 6 Micron or better and best full well capacity. 3. High speed data transmission 4. High frame rates in live mode with full resolution. 5. High resolution during acquisition mode. 6. High Dynamic range and low dark noise. Software for image acquisition and analysis: Windows based completely integrated software for single & Multichannel imaging, merging of images, acquisition in both DIC and Fluorescence, Measurements, Automatic exposure time adjustment, colour correction, live imaging mode with high frame rates and acquisition mode with high resolution, scale bar , Annotation, export of images to Tiff/JPEG format, brightness, gamma and contrast adjustment, different exposure times etc…The software should have all features essential for high end scientific imaging applications. Control Computer configuration: Latest high end branded system (Dell/HP) with latest Microprocessor, 8 GB DDR 3 RAM, 20” IPS LCD/LED Monitor, DVD R/RW, 2x500GB HDD RAID Configuration, Graphics Card with 1 GB RAM, Gigabyte Ethernet, Key board, Optical mouse etc… Optional items: 1. Ergo Binocular Tube with port for camera attachment 2. Spare metal halide lamps 3. Spare lamps for transmission light
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4. Immersion Oil Other Important clauses: 1. System should work with 220V@50Hz 2. Complete system should carry one year onsite comprehensive warranty. 3. The computer should carry three years onsite comprehensive warranty. 4. Complete Service and technical manuals with detailed documentation, user manual should be supplied along
with the system. 5. List of users of similar research institutions in India to be provided with addresses and contact details. 6. Technical presentation & demonstration of similar system in other research institutes / centers / universities
should be arranged on request. 7. Criteria for selection would be based not only on specifications, features, clauses and conditions but on proven
performance of Upright fluorescence Microscope and technical support. Therefore all the points mentioned in the tender should be answered.
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ITEM NO.9 – ADVANCED MOTORIZED INVERTED RESEARCH MICROSCOPE WITH CCD DIGITAL CAMERA, QTY: 1 NO. (FILE REF: 34/CCMB/APP/406/P12) Microscope: 1. Mechanically rugged and sturdy fully motorized Binocular Inverted Microscope with a side port for camera and motorised switchable Light path for Binocular: camera, 100:0, 0:100 and additional 30:70/80:20/50:50 light path. 2. All the components of Microscope like objectives, condenser, fluorescence filters, DIC accessories, light path switching etc… should be completely motorized. 3. All the microscope controls and other accessories like objectives, DIC accessories, Fluorescence filters, lamps, objective focus speed etc…should be controlled through programmable TFT/LCD Touch screen display for easy operation and also through computer. Control buttons should be provided on the microscope body for direct control of all the components of microscope. 4. The microscope should automatically adjust all the necessary components or accessories required for DIC/Fluorescence/ Phase/ Bright field applications with a touch of button on the touch screen. 5. The Microscope focus drive should have programmable upper limit function, such that the objective should not hit the slide/ petridish even when the user over drives the focus knob. 6. There should not be any pixel shift while acquiring DIC and Fluorescence imaging, both the DIC and Fluorescence images should exactly overlap. 7. The halogen lamp light intensity should automatically adjust as per the objective/ application like DIC or Bright field etc… 8. Fully Apochromatic corrected optical path for fluorescence. 9. In built motorized Z focus drive with minimum 15nm step size or better with coarse and fine mechanisms. 10. Objective nose piece with 6 positions or more. 11. Motorised neutral density filters for both bright field and Fluorescence. 12. Blue and green filters for transmission light path. 13. Motorized XY scanning stage plate with travel range 125 X 80 mm or more with resolution of 0.1microns or more and reproducibility of +/- 1 micron and should have computer interface and Joystick for manual operation X-Y stage with right hand movement control. 14. 12v -100w Halogen lamp for transmission light. 15. Objectives Plan achromat/Semi apochromat 4xPh / 5xPh, Long working distance 10x Ph & 40xPh, Plan Apochromat10x, 20x,40x, 60x/63x, 100x with high N.A. for Fluorescence and DIC applications with parfocality. 16. Long working distance Motorized Condenser for bright filed, Dark field, Phase and DIC application with N.A. 0.55 or more. 17. Eye piece 10x/22 or more adjustable. 18. DIC accessories for 10x, 20x, 40x, 60x/63x, 100x objectives. 19. High speed built in shutters for transmitted light and fluorescence light. 20. Variable field and aperture diaphragms for Bright and Fl applications. 21. 6 position Fl filter wheel, Fluorescence filters for DAPI, GFP (narrow band), FITC, CY3, CY5, m-cherry. 22. 120W/130W Metal halide lamp source with 1500 hours or more life time for Fluorescence applications with intensity control through computer and manual. 23. Special software and hardware modules for fast time lapse imaging applications 24. The system should be user friendly and more flexible for multi user environment. High end Digital camera: 1. High end 12-bit Scientific Digital cool CCD mono chrome camera for fluorescence applications with 1.4 Mega Pixel basic resolution. 2. Pixel size of 6 micron or better and best full well capacity. 3. High speed data transmission. 4. High frame rates in live mode with full resolution. 5. High resolution during acquisition mode. 6. High Dynamic range and low dark noise. Advanced Software for image acquisition and analysis: 1. Windows based completely integrated advanced software for full Microscope control, Multichannel imaging, Measurements, Automatic objects recognisation, Automatic exposure time adjustment, Quantification software, live imaging mode and high resolution mode, scale bar, export of images to Tiff/JPEG /Avi format, brightness and contrast adjustment, Different exposure times and Z focus positions for individual channels during multichannel acquisition, time lapse imaging, multiple position imaging with different X&Y coordinates, Z stack etc… 2. The software should have all advanced features essential for high end scientific imaging applications. 3. All the functions of the Microscope, Digital camera and other accessories like X& Y stage, Metal halide lamp should be controlled through a single integrated advanced software module.
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Control Computer configuration: Latest high end branded system (like Dell/HP) with latest processor, 8 GB DDR 3 RAM, 20”LED Monitor, DVD R/RW, 2x500GB SATA HDD, Graphics Card with 1 GB RAM, Gigabyte Ethernet, Key board, Optical mouse etc… Optional items: 1. Ergo Binocular Tube 2. The microscope with computer controlled IR LED/Laser based focus drift compensation mechanism for Z movement to get correct focus during long term time lapse imaging experiments. 3. Programmable and computer controlled incubation system for full automation. Compact incubator with CO2 controller and heating insert for slides, petridishes, 6well and 96 well plates should be quoted. Co2 controller and Temperature control unit should be controlled using the software. 4. Spare Lamps for Metal halide 5. Spare lamps for transmission light 6. LWD Semi apochromat 20x Ph,60x and 100x phase objectives, Immersion Oil Other Important clauses: 1. System should work with 220V@50Hz 2. Complete system should carry one year onsite comprehensive warranty. 3. The computer should carry three years onsite comprehensive warranty. 4. Complete Service and technical manuals with detailed documentation, user manual should be supplied along with the
system. 5. List of users of similar research institutions in India to be provided with addresses and contact details. 6. Technical presentation & demonstration of similar system in other research institutes / centers / universities should be
arranged on request. 7. Criteria for selection would be based not only on specifications, features, clauses and conditions but on proven performance
of Motorised inverted Fluorescence Microscope and technical support. Therefore all the points mentioned in the tender should be answered.
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ITEM NO.10 – SPECTRAL CONFOCAL MICROSCOPE, QTY: 1 NO. (FILE REF: 34/CCMB/APP/407/P12) SPECTRAL CONFOCAL MICROSCOPE FOR HIGH SENSITIVE AND HIGH RESOLUTION IMAGING OF BIOLOGICAL SAMPLES: The system should be of latest state of the art technology capable of high sensitive spectral confocal imaging of fixed & Live Biological samples. The system should include multichannel Fluorescence imaging with Z-stack, time-lapse, co localization, FRET, FRAP, photon counting, Photo activation and conversion. The system should consist of: 1. Fully Motorized Inverted Fluorescence Research Microscope for BF/DIC/FL with dedicated TFT/LCD display for full control
of Microscope. Motorized XY Scanning stage with universal sample holders for slides, Petri dish and other live cell sample holders like 6 well plate, 96 well plate for tile, mosaic and multipoint imaging. Motorized 6position DIC nosepiece, motorized BF/PH/DIC Condenser, 6 position motorized FL turret. High precision built in Zfocus drive with step resolution of 15nm or better. Transmitted and Reflected Light illumination with 100W halogen and 120W metal halide with PC control and long lifetime of 2000 hours. High resolution confocal grade Plan apochromat objectives corrected for both UV & VIS lines 10x/0.45, 20x/0.70,40x/0.85,40x/1.30oil, 60/63x/1.40oil,100x/1.4 oil immersion along with DIC accessories for all objectives. Bandpass fluorescent filters for DAPI, GFP,FITC, Cy3 and Cy5.
2. Anti vibration table. 3. Completely motorized Point scanning laser based Confocal unit with built in Internal high sensitive spectral detectors (Ga
AsP/HyD) based imaging for at least 2 channels in addition to the standard 2 channel PMTdetection with independent gain controls for all channels. All the FL detectors of the scan head should be filter free with freely selectable emission band width detection capability to suit to the emission spectra of the dyes. All detectors should be spectral. The detection unit should be inbuilt in the scanner unit. The system should include simultaneous detection and separation of at least 5 fluorophores based on spectral detectors. High sensitive motorized dichroic system with low angle of incidence with high efficient optics.
The system should be capable of recording emission spectra with minimum spectral resolution 5nm or better. Computer controlled continuously variable confocal pinhole with software control. Scan resolution of 6Kx6K or above. Frame rates of 7 fps or higher with spectral detectors @512x512 pixels in spectral detection mode. Real ROI scan and bleach with various ROI shapes should be possible for FRAP experiments. The scan field diagonal should be 18mm F.O.V. or more.
4. PMT based transmitted light detector for DIC imaging. 5. Laser unit with control electronics including multiline Ar 458/488/514nm, DPSS 559/561nm, HeNe 633nm & BDL405/408nm,
all laser lines including 405nm should be controlled through 8 channel AOTF for fast laser switching and attenuation. Seperate laser ports for UV/405nm, Vis, IR and additional port for future upgrades is essential. System should have capability to upgrade for tunable laser with visible wavelength.
6. Latest branded 64 bit control computer with Intel Xeon 6 Core Processor, DDR RAM 8 GB HDD: 1 TB SATA, DVD, Super Multi SATA +R/RW, Graphics : AT Fire GL V5200 256MB DH DVI, Gigabit Ethernet, Win 7 Ultimate 64 bit OS , USB 2.0, Fire wire. Large 30” LCD/LED/ TFT monitor.
7. Confocal system control software capable of controlling all motorized functions of microscope, scan head, lasers, AOTF including image acquisition & processing. Image acquisition for 3D, 4D, on-line spectral Imaging based on lambda stacks. Time series, FRAP, FRET, Photo activation and conversion. Imaging without bleed through and autofluorescence separation by online emission fingerprinting technique. Advanced multidimensional software for 3D analysis with Deconvolution, multichannel volume rendering, reconstruction, measurements across z stack, movie, co-localization with histogram analysis, intensity profiles for quantification etc....
Optional items: 1) Additional HeNe 594nm laser should be offered. 2) Upgradable to FCS/FCCS unit for Single Molecule Detection: The FCS unit with APD or equivalent detectors should be capable of performing auto and cross correlation measurements in live cells and solutions for a wide range of dyes and proteins. 3) The FCS unit should have facility for elimination/suppression of other excitation laser lines. All laser lines for Confocal Imaging
should be capable of working in FCS/FCCS mode. Dedicated Plan Apochromat 40x/1.1or more, or 63x/1.2 N.A. water should be offered along with the system. FCS measurement software for Auto and Cross correlation capabilities to be quoted.
4) Any patented detection system which is more sensitive and multi user friendly may also be offered. 5) Additional GaAsP/HyD detector or cooled GaAsP/HyD detector to be offered. 6) Additional Piezo/Galvo stage for Z axis to be offered. Other Important Clauses: 1. System should work with 220V@50Hz 2. Complete system should carry one year onsite comprehensive warranty. 3. Price for Additional 3 year warranty should be offered. 4. The computer should carry three years onsite comprehensive warranty. 5. In case of any problem with the system, the repairs should be carried out at CCMB and
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6. Necessary electronic components and modules should be sent to CCMB. 7. Applications training should be provided in CCMB by an applications specialist from the manufacturer’s facility. 8. Service training for one of the engineers from CCMB should be provided at the manufacturer site abroad for two weeks,
covering all maintenance, repair and calibration aspects of complete system with documentation details including schematics of scanning unit, control electronics etc… free of cost including travel, Lodging and living expenses.
9. Complete Service and technical manuals with detailed documentation, user manual should be supplied along with the system.
10. Tools necessary for calibration and alignment should be supplied along with the system. 11. All the specifications and features mentioned in the offer should be supported by technical literatures and compliance
statements with deviations clearly stated. 12. List of users of similar research institutions in India to be provided with addresses and contact details. 13. Technical presentation & demonstration of similar Confocal system in other research institutes / centers / universities
should be arranged on request. 14. Local service support available at Hyderabad should be mentioned with contact details. Response time for a service call
during and after warranty should also be mentioned. Number of years of the spare parts availability should be mentioned. 15. Offer should be complete in all respects. 16. Commitment & compliance of clauses should be explicitly stated wherever necessary. 17. Criteria for selection would be based not only on specifications, features, clauses and conditions but on proven
performance of Confcoal microscope and technical support. Therefore all the points mentioned in the tender should be answered.
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ITEM NO.14 – HIGH SPEED CENTRIFUGES, QTY: 2 NOS. (FILE REF: 34/CCMB/APP/411/P12) SPECIFICATIONS OF HIGH SPEED FLOOR MODEL REFRIGERATED CENTRIFUGE WITH ROTORS AND ACCESSORIES
I SPECIFICATIONS:
Maximum Speed : 28,000 rpm or more
Maximum g Force : 100,000 x g or more
Control and Display : Microprocessor with Digital display
Speed Control Accuracy : +/- 25 of set speed
Temperature Range : 0 to 40 C
Temperature Control : +/- 2 C of set temperature
Accelerations/Decelerations profiles : up to nine or more profiles
Refrigeration System : Non-CFC Refrigeration technology
Capacity : 4 litres or more
Sound level : < 59 dba
Ambient temperature range of operation : 35 C or more
Power : 220 Volts, 50Hz, (Single Phase)
II Other essential Features:
- DC Brushless Motor Air cooled Drive System or other latest technology
- Imbalance tolerant Drive.
- Automatic Run Recalls.
- Ergonomic design for ease of use.
III Rotors:
Fixed Angle:
1. Capacity : 8x50ml
Maximum Speed : 28,000 2 Nos.
RCF : 100,000 g or more
2. Capacity : 6 x 250ml
Maximum Speed : 14,000 RPM or more.
RCF : 30,000 g or more. 1 No.
3. Capacity : 6 x 500ml
Maximum Speed : 10,000 RPM or more. 1 No.
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RCF : 18,000 g or more.
4. Swinging Bucket Rotor:
Capacity : 6 x 50 ml
Maximum Speed : 13,000 RPM or more. 2 Nos.
RCF : 26,000 g or more.
Optional Swinging Bucket Rotor:
Capacity : 6 x 38 ml
Maximum Speed : 24,000 RPM or more. 2 Nos.
RCF : 100,000 g or more.
Buckets for running 15 ml tubes to be also offered.
Important Note:
1. Required tubes and bottles (Polycarbonate, Polypropylene, Polyallomer) with Adaptors, Caps etc.,
for 1.5ml, 10ml, 30ml and 50ml capacities for each rotor to be offered separately as optional items.
2. Cost should include three years warranty on drive, five years on refrigeration system.
3. Commitment for the supply of Complete Instruction, Service Manuals with Circuit diagrams and
engineering details (including Refrigeration) to be supplied along with the system. Commitment to
be made in the offer.
4. List of essential Spares parts with cost required for the trouble Free Operation of the system during
and after warranty to be given separately with cost as option.
5. Spare parts should be available for a period of 8 years of more. Commitment should be made in the
offer.
6. The Highcentrifuge system should have all safety features incorporated.
7. The offered model should be the latest and Start of Art in design and technology.
8. Availability of local Service Support and response time for a Service Call during and after warranty
to be mentioned.
9. Original Literatures containing detailed specifications and features of both the centrifuge and rotors
should accompany the offer.
10. List of users in India with Addresses, e-mail id’s and contact phone numbers should be enclosed
separately
11. All other terms and conditions of payment, delivery etc., to be mentioned clearly.
Criteria for selection would be based not only on the specification, features and safety but on the
proven performance of proposed systems suited to the applications and requirement of CCMB.
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ITEM NO.15 – FLOOR MODEL ULTRACENTRIFUGE, QTY: 1 NO. (FILE REF: 34/CCMB/APP/412/P12) FLOOR MODEL ULTRACENTRIFUGE WITH ROTORS AND ACCESSORIES Applications: Separation of Subcellular Particles, Varus and Viral Particles, Rate-Zonal Separation Proteins in Sucrose Gradient, Separation of Lipoproteins, Pelleting RNA through a CsCl Gradient and Isopycnic Separation of Plasmid DNA. I SPECIFICATIONS:
Maximum Speed : 100,000 rpm or more
Maximum g Force (RCF) : 800,000 x g or more
Control and Display : Microprocessor with Large touch-screen for user friendliness and convenience
Speed Control Accuracy : +/- 10 rpm or less of set speed
Refrigeration System : Thermoelectric cooling for lower power consumption
Temperature Range : 0 to 40 C with 1C steps
Temperature Control : +/- 0.5 C of set temperature
Ambient temperature range of operation : 20C to 35C or more
Sample imbalance tolerance : + 5 ml or 10% whichever is greater.
Drive cooling : Air Cooled for lower power consumption and maintenance Accelerations/Decelerations profiles : 10 Acceleration/Deceleration or more profiles
Sound level : < 55 dBa or less
Heat Output : Should be less than 4000 BTU/hr
Power : 220 Volts, 50Hz, (Single Phase) with wide tolerance for power fluctuations.
II Essential Features:
- System should automatically check for possible errors with rotor induced parameters and
automatically make necessary correction.
- Ultracentrifuge should be able to accept Fixed Angle Rotor, Vertical Rotor, Near Vertical Rotor or
equivalent Swinging Bucket Rotors.
- Samples volumes for centrifugation from 5 ml to 1500 ml.
- Adaptors to accommodate small volume samples to large volumes should be available without
sacrificing the maximum g force of rotors.
- Ability to remove moisture with vacuum.
- Automatic restart with set parameters after power interruptions.
- Multi steps users programs.
- Simulation software provision.
- Speed / temperature vs. time plot.
- All regulatory and safety standards to be complied.
- Many user settable programs. - Provision for Bio-safety features/upgradation to be offered as option.
III Rotors:
Fixed Angle Rotor :
1. Capacity : 8 x 38 ml or more
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Maximum Speed : 65,000 rpm or more 1 No.
Maximum g force : 429,000 g or more
K factor : 52 or less
The rotor should be able to accommodate low volumes such as approximately 25ml or 15 ml with adapters and without compromising the Maximum rcf of 500,000 g or more.
Near Vertical Rotor :
2. Capacity : 8 x 5 ml or more
Maximum Speed : 100,000 rpm
Maximum g force : 7,50,000 g or more 1 No.
K Factor : 9 or less
The rotor should be able to accommodate lower volumes or approximately 2ml with adapters and without compromising the Maximum rcf of 750,000 g or more.
Swing out Bucket Rotor :
3. Capacity : 6 x 13 ml or more
Maximum Speed : 41,000 rpm
Maximum g force : 2,80,000 or more 1 No.
K Factor : 125 or less
The rotor should be able to accommodate lower volumes or approximately 3.5ml with adapters and without compromising the Maximum rcf of 280,000 g or more.
4. Capacity : 6 x 38 ml or more
Maximum Speed : 30,000 rpm or more
Maximum g force : 166,000 or more
K Factor : 220 or less
The rotor should be able to accommodate lower volumes or approximately 25ml or 15ml with adapters and without compromising the Maximum rcf of 175,000 g or more.
Important Note:
1. All required tubes and bottles (Polycarbonate, Polypropylene, Polyallomer, Quick seal) with Adaptors, Caps
etc., for each rotor to be offered separately as optional items.
2. Cost should include three years warranty on the complete system, five years on drive assembly and rotors.
3. Commitment for the supply of Complete Instruction, Service Manuals with Circuit diagrams and engineering
details (including Refrigeration) to be supplied along with the system. Commitment to be made in the offer.
4. List of essential Spares parts with cost required for the trouble Free Operation of the system during and after
warranty to be given separately with cost as option.
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5. Demonstration of the Ultracentrifuge with rotors to be done on request by CCMB. Also sample runs to be
made if required.
6. The Ultracentrifuge system should have all safety features incorporated.
7. The offered model should be the latest and Start of Art in design and technology.
8. Spare parts should be available for a period of 8 years of more. Commitment should be made in the offer.
9. Availability of local Service Support and response time for a Service Call during and after warranty to be
mentioned.
10. Original Literatures containing detailed specifications and features of both the centrifuge and rotors should
accompany the offer.
11. List of users in India with Addresses, e-mail id’s and contact phone numbers should be enclosed separately
12. All other terms and conditions of payment, delivery etc., to be mentioned clearly.
Criteria for selection would be based not only on the specification, features and safety but on the proven
performance of proposed systems suited to the applications and requirement of CCMB.
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ITEM NO. 17 - ZEBRA FISH IMAGING AND TRACKING SYSTEM, QTY: 1 NO. (FILE REF: 34/CCMB/APP/414/P12)
I. Specifications:
1. CCD Camera : Scientific grade high resolution IR Sensitive monochrome CCD camera
along with zoom lens, suitable Frame-grabber card, power supply
cables etc. (Make, Model & other Details of camera to be specified).
2. Hood : Vibration free Light tight enclosure for above camera and sample plate
holder In 96 well plate format; uniform backlit IR light source; upper white light illumination
etc.
3. Software features : Video tracking software for automated behavioral research of Zebra fish
and Larvae including analyzing the movement, activity of individual as well as social behavior
of multiple experimental specimen, Data analysis visually and statistically, provision for live
video recording and backup facility etc.( Any other special features may be clearly mentioned)
4. Throughput : Single and multiple tracking of approx.100 arenas simultaneously and
up to 16 arenas in open field at the same time.
5. Computer: : Latest Desktop branded PC (Dell / HP) suitable for the above system
with 6GB RAM, 2 X 500GB HDD, DVD Writer, 23” LCD Monitor, Mouse,
Keyboard, Network interface, Windows XP / Windows 7 OS etc.
d) Single user as well as Multi user Network licenses (optional) to be offered.
14. Hardware and : a) Windows 7 (64 bit) or better operating system with latest
Computers hardware configurations like high RAM (> 8GB),
highest screen resolution, 24” LCD display or better, Gigabit
Ethernet card and other interface cards as required.
b) Suitable and compatible Servers, PC’s with high storage
capabilities to be offered optionally for data analysis.
15. Live Cell Chamber : For providing required Temperatures & CO2 Controls for live samples.
16. Transmitted Light : For label free monitoring using Bright Field / Phase Contrast and DIC.
III. Optional Items:
a) Range of excitation and emission Filters in addition to what is specified under item 4 filters (which should be
standard).
b) Objectives other than standard that are supplied with the systems
c) Data visualization tools, software for database, 3 D analysis etc..
d) Any software other than standard supply
e) Multiuser / Network licenses for all software to be offered.
f) Compatible and suitable servers, PC’s colour Laser Printers and storage devices required for in-depth analysis
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and archival.
g) Suitable Slides and multiwall plates (96 and 384 well plates) for various applications.
h) Other consumables required for the operation of the system.
i) Any other accessories required for making the System complete.
IV. Important Clauses: 21) The system and accessories should be compatible for operation at 220/230VAC 50Hz.
22) Servers, Computers and printers offered along with the system should be the latest models compatible with the application software.
Required interface cards, cables etc. to be included along with storage devices and CD writers.
23) The PC configuration offered should be specified and should include a three year comprehensive onsite warranty.
24) Detailed literatures (all Originals) with technical specifications and features for the main system and all the accessories to be enclosed
with the offer.
25) Please ensure that the specifications mentioned in the offers must cover all the parameters listed in our enquiry. Unspecified
parameter will be treated as non-complaint.
26) Detailed Instructions and service manuals for the entire system and accessories should be supplied along with the system. Commitment
to this clause to be mentioned in the offer.
27) Software supplied should be the latest versions and any up gradations in the Software to be given free of cost for three years.
28) All standard accessories that would be supplied with the system should be clearly mentioned in the offer. Other required accessories
may be offered separately as options.
29) The principals / local agents are responsible for the installation, testing and commissioning of the main system and accessories which
should be carried out immediately after receipt of the system. Integration of the main system with the accessories, checking the
specifications, training the users to be done at the site free of cost.
30) Please indicate the year in which the model was introduced in the market and confirm whether the spares and consumables for the
system would be available for a minimum period of 10 years.
31) Pre-installation and utility requirements for installation and running the system should be clearly mentioned.
32) Availability of local service support and response time for a service call during and after warranty to be specified.
33) Alternate models, accessories and options if any to be offered separately with break-up-costs and indicating differences / advantages
between various models.
34) Warranty for the entire system and accessories to be clearly mentioned. A two years comprehensive extended warranty cost including
parts and labour to be included in the option in addition to the standard warranty.
35) Copies of Scientific papers, references where similar models as the offered system has been used should be enclosed.
36) List of users in India of the similar models as the one(s) offered along with the names, addresses, telephone numbers and mail ID’s to
be enclosed separately.
37) Demonstration of similar models as the one(s) offered to e given on request at other customer sites.
38) In-depth service training on the repair and maintenance of the system to be provided for two Instrumentation personnel of CCMB at
the manufacturers facilities. The training to be offered free of cost including the to and fro airfare from Hyderabad to the place of
training, boarding, lodging and all living expenses.
39) All other clauses of payment details validity of quotation, delivering schedule, shipment etc… to be indicated.
40) Technical presentations of the systems offered are to be made on request from CCMB.
41) Typical samples provided to be imaged and analyzed on similar systems at other customer sites and given to CCMB for evaluation.
42) Selection of the system would be based on the response to all the above points apart from technical specifications, features, proven
support & service and suitability to CCMB’s requirements. Please therefore respond to all the points.
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ITEM NO.19 – HIGH THROUGHPUT SNP GENOTYPING AND GENE EXPRESSION SYSTEM, QTY: 1 NO. (FILE REF: 34/CCMB/APP/416/P12)
Applications:
Genome wide genotyping and custom genotyping.
Whole genome gene expression.
Whole genome methylation analysis.
CNV analysis.
Specifications and Features:
Dual laser excitation source and optics for scanning high density arrays with high resolution, fast scan times and to produce high quality reproducible data.
Scanner resolution should be 0.3 micron or better. Should be able to scan different types of arrays/chips with fast scan times ranging from 1 minute to 15
minutes. The system should include a Hybridization unit, fluidics station and autoloader for processing and scanning
the arrays/chips. Platform should be amenable for assay automation. The scanner should be the latest technology and should be able to carry out sensitive, rapid and accurate
imaging of array/chips. The system should be modular and configurable for any level of throughput required. The system should include the basic control software and suitable analysis software (minimum of 5 user
licenses) for all the above mentioned applications. Complete set of accessories needed for processing the arrays/chips for all the applications to be offered. The system offered should be the latest and advanced version having all the
specifications and features mentioned above and should be of use for CCMB’s research programs for the
next eight years or more.
Scientific papers, references in reputed journals where the offered model has been used for the stated applications in the world (for the last three years or more) should be enclosed.
For each of the applications, One kit, along with sufficient consumables and reagents required for utilising one kit, to be included with the system
To offer optionally:
Upgrade for Next Generation Sequencing platform including suitable fluidics, kits and reagents and required
software for control and data analysis.
Liquid handling accessory for assay automation of array /chips with required consumables, reagents,
software etc.
Pre arrayed and custom array /chips for the above applications.
Kits, reagents other accessories needed for all the above applications.
Required servers/ workstations for downstream data analysis.
Important clauses:
Main system including subsystems, computers and accessories should operate on 220V, 50 Hz mains power.
The computers / workstations and printers offered along with the system should be the latest model (configuration to be specified) compatible with the control and analysis software. Required interface cards, cables, CD writer etc to be included. The PC /workstation should have a three year comprehensive onsite Warranty.
Detailed literatures (all originals) with technical specifications and features to be sent along with the offer.
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Detailed instruction and service manuals with circuit diagrams and all engineering details for the entire system should be supplied with the unit. Commitment to this clause to be made in the offer.
Software supplied should be the latest versions and any upgradation in the software to be given free of cost for three years.
Required calibration kits for the installation and testing should be supplied with the system. If required demonstration of the unit to be given at any installed sites in India. All standard accessories that would be supplied along with the unit should be clearly mentioned in
the offer. Other required accessories should be offered as optionally. Essential electronic, mechanical spares and consumables to be offered for a minimum period of six
months of operation. Detailed list of such items to be given separately. Complete installation and testing the equipment to its specifications should be done at the site of
installation free of cost. After the installation, the system should be demonstrated to the users and samples should be run on the instrument and tested. User training to be given at site and periodic application training to be given till the users are familiar with the system and software. Commitment to this effect to be made in the offer
Cost offered should include a two-year warranty on the main system inclusive of parts and labour. Service training for one of the instrumentation staff of CCMB should be provided at the
manufacturing facilities abroad. This should be an extensive training programme covering the repair, maintenance and calibration aspects of the system and should form a part of the official service training course at the manufacturing facilities abroad. The above training to be offered free of cost including tution fees, To and fro airfare from Hyderabad to the place of training, boarding, lodging and all living expenses.
Availability of local service and application support and response time for a service call during and after warranty to be specified.
Spares for the system should be available for a minimum period of 7 years and confirmation to be given in the offer.
The year in which the offered model was introduced into the market and the number of installations in India and all over the world over the last three years should be mentioned.
List of users worldwide and in India along with the names, email ID’s and telephone numbers of the customers to be enclosed in the offer in separate annexures.
All other clauses of warranty, delivery schedule, payment details to be clearly mentioned Apart from meeting the specifications, features and clauses, selection of the system would also
be based on the response to all the points mentioned above and hence please respond to all the
points.
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ITEM NO.13 – HIGH THROUGHPUT REAL TIME PCR SYSTEMS, QTY: 3 NOS. + 2 NOS. (FILE REF:
34/CCMB/APP/410/P12)
I) High throughput Real time PCR Systems --- 3 nos Applications: a) Gene Expression studies
b) Absolute quantitation and relative quantitation c) Allelic discrimination d) Genotyping e) HRM analysis
Specifications:
Block Format 96 well / 384 well interchangeable.
Fluorescence excitation and 450 nm to 650nm Emission range 500 nm to 700 nm
Thermal cycling system Peltier based
Temperature range 4 ºC to 100 ºC
Average Ramping rates 3.0 C/sec or better
Temperature accuracy ± 0.3 ºC or better
Temperature uniformity ± 0.5 ºC or better
Sensitivity Detection down to one copy
Reaction volume 3 to 20 microlitres for 384 well plates
Standard features required: User interchangeable blocks 384 well to 96 well blocks with no calibrations requirements for every block change.
Multiplexing capability up to four colors or more.
System should be supplied with a 384 well block.
Software for all the above mentioned applications, primers & probes design software etc with multi user license to be included with the system.
Provision to add new filters/ dyes.
Provision to run with or without passive reference dye.
The system should be compatible for use with third party consumables and kits.
Should be able to store at least 100 or more data files.
Maximum run time should not be more than 80 to 90 minutes with standard kits and not more than 40 minutes with fast reagents and consumables.
Should be able to upload/download protocols, data files etc using an USB interface.
Provision for sensitized emission based measurements for at least four/ five colors with independently selectable emission and excitation filters.
II) High throughput Real time PCR Systems for all the above applications --- 2 nos
with all the specifications, standard features as given in item I and in addition having the following features
System offered should be upgradable to high density array block at any time.
Should be FDI approved and validated for diagnostic applications.
III) To offer Optionally (for items I & II) Kits and reagents for Real Time PCR, SNP Genotyping.
Reagents and consumables for fast runs
96 well block.
High density array block.
384 well plates compatible with the above systems.
Required kits for diagnostic applications for item (II).
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Important clauses:
a. Main system including subsystems, computers and accessories should operate on 220V, 50 Hz mains power. b. The computer and printer offered along with the system should be the latest model (configuration to be
specified) compatible with the control and analysis software. Required interface cards, cables, CD writer etc to be included. The PC and should have a three year comprehensive onsite Warranty.
c. The lamp lifetime should be specified. d. Detailed literatures (all originals) with technical specifications and features to be sent along with the offer. e. Detailed instruction and service manual with circuit diagrams and all engineering details for the entire
system should be supplied with the unit. Commitment to this clause to be made in the offer. f. Software supplied should be the latest versions and any up gradation in the software to be given free of cost
for three years. g. All standard accessories that would be supplied with the unit to be clearly mentioned in the offer. Other
required accessories should be offered separately. h. Essential electronic, mechanical spares, consumables and required reagent kits to be offered for a minimum
period of six months of operation. Detailed list of such items to be given. i. Complete installation and testing the equipment to its specifications to be done at the site of installation free
of cost. User training to be given at site and periodic application training to be given till the users are familiar with the system and software.
j. After the installation the system should be demonstrated to the users and user samples to be run on the instrument and tested. Additional training on the instrument and the software to be provided to the users as and when needed and commitment to this effect to be made in the offer.
k. Required calibration kits for the installation and testing should be supplied with the system. l. Spares for the system should be available for a minimum period of 8 years and confirmation to be given in
the offer. m. Cost offered should include a two-year warranty on the main system inclusive of parts and labor. n. Availability of local service support and response time for a service call during and after warranty to be
specified. o. If required demonstration of the unit to be given including running some user samples for proper
assessment of data quality. p. The system offered should be the latest advanced version having all the specifications and features
mentioned above and should be of use for CCMB’s research programs for the next eight years or more. q. To indicate the year in which the offered model was introduced into the market and the number of
installations in India and all over the world over the last three years. r. List of users worldwide and in India along with the names and telephone numbers of the customers to be
enclosed in the offer in separate annexure. s. Scientific papers, references in reputed journals where the offered model has been used for the stated
applications in the world (for the last three years or more) should be enclosed. t. All other clauses of warranty, delivery schedule, payment details to be clearly mentioned
Apart from meeting the specifications and needs, selection of the system would also be based on the
response to all the points mentioned above and hence please respond to all the points.
