CHARACTERIZATION OF NATURAL AND ENGINEERED NANOPARTICLES: SHAPE, SIZE
AND CHEMICAL COMPOSITIONLucia Manangon
http://www.theismaili.org/cms/317/Building-the-future-one-nanoparticle-at-a-time
NANOPARTICLES
Ferrihydrite
Natural nanoparticle
CNT
0.25 µm
100 nm
TEM image of 20 nm Au NP
Engineered nanoparticle
Poda et al., J. Chromatogr. A. 2010, 1218 (27), 4219-4225.
NANOPARTICLES IN COMERCIAL PRODUCTS
FATE ??BEHAVIOR ??
BIOAVAILABILITY ??TOXICITY ??
Characterization and Analytical Techniques
SizeShape
Composition
• Field Flow fractionation (FFF)
• Multi Angle Light Scattering (MALS)
• Dynamic Light Scattering (DLS)
• Inductively coupled Plasma (FFF-ICP-MS)
FFF
FRACTIONATION
MULTI ANGLE LIGHT SCATTERING (MALS) – DYNAMIC LIGHT SCATTERING (DLS)
incident beam
scattered light
SIZE AND SHAPE
ICP-CC-MS
Li
C
Na
Mg
Al
Si
P
S
K
Ca
Ti
Mn
Fe
Co
Ni
Cu
Zn
As
Se
Sr
Mo
Ag
Sb
Cs
Hg
Pb
U
27 elements are analyzed:
CHEMICAL COMPOSITION
Online system
ICP - MS
FFF
UV
MALS - DLSFLUORESCENCE
SAMPLES
Au, AgPS microspheresCNT
Natural nanoparticle
Engineered nanoparticle
GSLPore water Deep brine layerDeep brine sediment
FRACTIONATION SIZE AND SHAPE CHEMICAL COMPOSITION
UNDERSTANDING OF FATE, BEHAVIOR, BIOAVAILABILITY AND TOXICITY
