Pratip K. Chattopadhyay, Ph.D.

InnovativeLeading multidisciplinary team that developed 30-parameter flow cytometry (2014 - )Leading single cell RNA sequencing effort (2014 - )Introduced Brilliant Violet dyes to flow cytometry (2012)Led NIH evaluation of new mass cytometry technology (2010)Central role in the development of six novel bioinformatic tools (2010-2014)Optimized 96-parameteOptimized 96-parameter, single cell transcriptomic technology for immune monitoring (2010)Performed largest clinical research studies to date that used multicolor flow cytometry Developed novel (widely used) method for purification of antigen-specific CD4+ T-cells (2005)Inspired the development of SPICE data analysis softwareIntroduced Quantum Dots for use in flow cytometry (2005)Lead author, first report of 20-parameter flow cytometry (2005)

Leader60+ national and international invited talks since 200610 high profile keynote or plenary speaking invitations since 2010Work profiled on BioLegend, Invitrogen, Fluidigm, and BD BioSciences websitesWork highlighted by Faculty of 1000, College of American Pathologists (CAP) Today, Genetic Engineering News, Nature Technology Feature, NIH Intramural Research Program advertisingDuke University Center for AIDS ReseaDuke University Center for AIDS Research Visiting Professor (2010)ISAC Scholar (2012 - )NIH Special Service Award (2007)Chair and course organizer of full-day courses on multicolor flow cytometry and advanced data analysisCo-developed new manuscript category for journal (Cytometry Part A)Key developer of CYTO U (on-line education for flow and image cytometry), leader of e-delivery task force

pchattop@mail.nih.gov703-732-6096

Productive50 publications over 12 years as a post-doctoral and independent researcher

Top tier journals (Nature Medicine, Nature Protocols,Nature Immunology, Cell, Immunity, Blood)

h-index = 25 (25 papers with at least 25 citations),i10-index = 38 (38 papers with at least 10 citations)

Citation count, July 2015: 2938Citation count, July 2015: 2938Patent Application : A Small Laboratory Device

to Automate and Expedite Cell Thawing.

Pratip K. Chattopadhyay, Ph.D.

Current Position

Staff Scientist (2008 - )ImmunoTechnology Section

Vaccine Research Center, National Institutes of Health

Narrative

Immune responses are incredibly complex, involving the coordination of many effector molecules,

expressed across a wide variety of cell types. Because of this complexity, the cell profiles that predict

effective immune responses are often unclear, particularly early after infection or vaccination.

My work aims to identify the T-cell responses that provide early biomarkers for effective immunity.

I do this by developing and applying cutting-edge technologies that characterize - at the single cell level -

immune cells fimmune cells from well-defined clinical settings (vaccination, natural HIV infection, lymphoma). Using

highly multiplexed transcriptomic and proteomic approaches, I interrogate cells in an unprecedented,

comprehensive manner. My goal is to reveal clear, clinically relevant, and actionable “multidimensional”

biomarkers. This work requires multi-disciplinary expertise, and the ability to lead cross-functional teams

that include clinical researchers, basic scientists, engineers, and computational biologists.

pchattop@mail.nih.gov703-732-6096

Academic Training

Post-doctoral and Research FellowImmunoTechnology Section

Vaccine Research Center, National Institutes of Health

Mentor: Mario Roederer

Ph.D., Molecular Microbiology and ImmunologyJohns Hopkins UniversitJohns Hopkins University, School of Public Health

Mentor: Joseph B. Margolick

Thesis: Ineffective T-lymphopoeisis Underlies

T-cell Homeostasis Failure in HIV-1 Disease

B.A., BiologyUniversity of Virginia

Independent Research: 1) Signaling Pathways

and and T-cell Morphology, 2) γδ T-cells in GVHD,

3) In vitro assembly of Herpes Simplex Virus VP26 Capsids