102
ON HPLC COLUMNS Chemical Materials Organic Chemistry
ON HPLC COLUMNS
Chemical MaterialsOrganic Chemistry
No.TI049E
TI049E-CB12
Highly-Efficient Separation of Surfactant
Triton X-100, a surfactant including a poly-oxyethylene chain with a different degree of polymerization, is a difficult chemical compound ODS column separation.
Chromatogram (a) is a separation by a conventional 5um column and the degree of polymerization recognition is insufficient. Chromatogram (b) shows that Cadenza CD-C18 particularly improves separation. Moreover, Chromatogram (c) shows a nearly perfect separation achieved by connecting two Cadenzas with each other.
Cadenza CD-C18's impressive ability to recognize molecules in the stationary phase and outstanding 50,000 plates lends itself to improve recognition of oxy-ethylene within Triton X-100.
Cadenza CD-C18 pushes the envelope of history to open up a new era of high separation ability in the reversed-phase analysis mode.
ACN / water = 65 / 35, 0.8 mL/min, UV at 254 nm
Conventional 5µm ODS
250 x 4.6 mm (b)
min0 5 10 15 20 25 30
mAU
0
10
20
30
40
min0 5 10 15 20 25 30
mAU
0
10
20
30
40
min0 10 20 30 40 50 60
mAU
0
10
20
30
40(a) (c)250 x 4.6 mm ( 250 x 4.6 mm ) x 2
C8H17 OCH2CH2 nOH
Triton X-100
6.3 MPa 12.3 MPa 22.2 MPa
No.TI016E
TI016E-AA16
Cadenza CD-C18ACN / water / acetic acid = 60 / 40 /0.1UV at 260 nm, 37 °Cacetylsalicylic acid from tablet5 mg/mL, 0.4 uL
Acetylsalicylic acid from Tablet
O
COOHOCCH3
Acetylsalicylic acid
min0 1 2
mAU
0
100
200
300
400
min0 1 2
mAU
0
100
200
300
400
1.0 mL/min5.0 MPa
0.5 mL/min4.3 MPa
0.2 mL/min4.2 MPa
min0 1 2
mAU
0
100
200
300
400
No.TI017E
TI017E-AA19
Cadenza CD-C18, 150 x 4.6 mmACN / water / formic acid = 90 / 10 /0.050.8 mL/min, 37 °CUV at 215 nm, 3.7 MPa
cis - trans Isomers of 9-octadecenoic acid
COOH
COOH
min0 5 10 15 20
mAU
0
10
20
12
elaidic acid
oleic acid1
2
No.TI018E
TI018E-AB15
Cadenza CD-C18, 75 x 4.6 mmacetonitrile / water / formic acid = 80 / 20 / 0.051.0 mL/min, 37 °CUV at 220 nm, 2.6 MPa
Dyes
1
2
3
1
2
3
min0 1 2 3 4
mAU
0
25
50
75
100
methyl yellow
sudan red G
indophenol
OHNO
NNNCH3
CH3
NN
CH3 HO
No.TI025E
TI025E-AB02
Cadenza CD-C18, 75 x 4.6 mm acetonitrile / 5mM beta-cyclodextrin = 10 / 901.0 mL/min, 7.2 MPa, UV at 260 nms, 37 °C1.0 uL/mL x 1.0 uL
Cresol isomers
p-cresol
m-cresol
o-cresol
H3C OH
H3C
OH
CH3
OH
1.
2.
3.
min0 5 10 15 20 25 30
mAU
0
20
40
60
12
3
No.TI029E
TI029E-AC15
Cadenza CD-C18, 75 x 4.6 mmmethanol 1.0 mL/min, 37 °C3.2 MPaUV at 210 nm
Squalene
min0.0 2.5 5.0 7.5 10.0 12.5 15.0
mAU
0
100
200
CH3 CH3
CH3
CH3
H3C
H3C
CH3CH3
No.TI031E
TI031E-AC19
Cadenza CD-C18MeOH / water / formic acid = 30 / 70 / 0.137 °C, UV at 260 nm
Isomers of Phthalic Acid
1
2
3
p-phthalic acid( terephthalic acid )
o-phthalic acid
m-phthalic acid( isophthalic acid )
min0 2 4 6 8 10
mAU
0
100
200
300
1
2
3
COOH
HOOC
COOHHOOC
COOH
COOH
min0 2 4
mAU
0
200
400
600
1
2
3
min0 2 4
mAU
0
200
400
600
1
2
3
1.0 mL/min, 9.5 MPa
1.0 mL/min, 4.3 MPa 0.4 mL/min, 3.4 MPa
No.TI033E
TI033E-AD16
Cadenza CD-C18methanol / 20 mM CH3COONH4 = 20 / 801.0 mL/min, 37 °C, UV at 230 nm1. benzoic acid 0.5 mg/mL2. sorbic acid 0.25 mg/mL 1.0uL inj.
Food Preservatives
1 2
min0 1 2 3 4 5
mAU
0
100
200
300
400
1
2
min0 1 2 3
mAU
0
100
200
300
400
12
COOH
benzoic acid
O
OHsorbic acid
8.7 MPa 6.2 MPa
No.TI043E
TI043E-AF01
Cadenza CD-C18250 x 4.6 mmACN / TFA = 100 / 0.1 1.0 mL/min, 37 °C6.9 MPa, UV at 210 nm1.0 uL injection
Impurities in Hexane
min0 2 4 6 8 10
mAU
-5
0
5
10
15grade "Guaranteed Reagent"
min0 2 4 6 8 10
mAU
-5
0
5
15grade "for environmental analysis"
No.TI044E
TI044E-AF16
Cadenza CD-C18, 250 x 3 mmhexane / 2-propanol = 40 / 600.4 mL/min, 30 °CUV at 325 nm15.3 MPa
Fullerene and Higher Fullerenes
min0 10 20 30 40 50 60
mAU
0
250
500
750
1000
C60
C70
min0 10 20 30 40 50 60
mAU
0
2
4
6
8C60 C70
higher fullerenes
Courtesy of Dr. Naoki Komatsu, Kyoto Univ.
No.TI048E
TI048E-AG13
Cadenza CD-C18, 50 x 4.6 mm acetonitrile / water = 50 / 501.0 mL/min, 4.4 MPa, 37 °C, UV at 260 nm1uL injection,0.1% solution
Dichlorobenzene isomers
1.
2.
3.
min0 2 4 6 8 10
mAU
0
2
4
6
1 2
3
p-dichlorobenzene
m-dichlorobenzene
o-dichlorobenzene
Cl Cl
Cl
Cl
Cl
Cl
No.TI066E
TI066E-BC07
Cadenza CD-C18, 100 x 4.6 mmwater / methanol = 30 / 701.0 mL/min, 13.2 MPa37 °C, UV at 210 nm
Indoor air pollutants
min0 2 4 6 8 10 12
mAU
0
20
40
60
80
100
120
1
2
3
45
6
7
89
10
p-xyleneH3C CH3
tolueneCH3
benzene
m-dichlorbenzeneCl
Cl
o-dichlorbenzeneCl
Cl
styreneCH CH2
ethylbenzeneCH2CH3
tetrachlorethyleneC CCl
ClCl
Cl
trichlorethyleneC CCl
HCl
Cl
chloroformC Cl
H
Cl
Cl1
2
3
4
5
6
7
8
9
10
No.TI069E
TI069E-BD25
Cadenza CD-C185 mM CH3COONH4 / ACN = 40 / 6037 °C, UV at 260 nm, 2.0 uL inj.
Fungicides for Citrus Fruits
1
2
3
4
N
NH
S
N thiabendazole
imazalil
o-phenylphenol
diphenyl
Cl
Cl N
N
O
OH
min0 2 4 6 8 10
mA
U
0
50
100
150
200
250
300
350
4001 2
3
4
1.0 mL/min3.3 MPa
0.43 mL/min3.0 MPa
0.2 mL/min3.0 MPa
No.TI075E
TI075E-BI11
150 x 4.6 mm20 mM Na2SO4 / acetonitrile / acetic acid = 45 / 55 / 11.0 mL/min, 40 °C, UV at 254 nm
Impurities separation for pharmaceutical compound
Courtesy of Hiroshi Okumura
Brand-A
No.TI089E
TI089E-CF03
acetonitrile / water / phosphoric acid = 600 / 400 / 1, 40 °C, 280 nm
Column size comparison for elution of investigational medication
Courtesy of Hiroshi Okumura
Brand-A150 x 6 mm
1 mL/min500 µL inj.
0.2 mL/min500 µL inj.
0.4 mL/min500 µL inj.
No.TI091E
TI091E-CF04
Cadenza CD-C18, 100 x 2 mm80% methanol, 0.2 mL/min, 40 °C, 5µL(1pg/µL)API4000: ESI, MRM Negative, Q1/Q3 : 205 / 106
LC-MS Application for Endocrine Disruptor
Courtesy of J.Watanabe, TAKARA BIO INC.
0
20
40
60
80
100
120
140
160
180
200
220
240
260
280
300
320
340
360
OH C8H17
4-octylphenol (Mw. 206.33 )
1 2 3 4 5 6 min
API4000 (TAKARA BIO INC. )
5 pg
No.TI093E
TI093E-CF04
Cadenza CD-C18, 50 x 2 mm0.1% formic acid / 60% methanol, 0.2 mL/min, 40 °C, 5µL (20fg/µL)API3000: ESI, MRM PositiveQ1/Q3: 192/119
LC-MS Application for Bug Repellent Active Ingredient
Courtesy of J.Watanabe, TAKARA BIO INC.
0.5 1.0 1.5 2.0 2.5 3.0 min
API3000 (TAKARA BIO INC. )
0
100
200
300
400
500
600
700
800
900
1000
11001153
100 fg
N
H3C
O
H3CCH3
DEET (N,N-diethyl-m-toluamide)Mw. 191
No.TI107E
TI107E-CA08
water / formic acid = 100 / 0.137 °C, UV at 230 nmacrylamide, 1mg/mL x 1uL
Acrylamide
OCH CH2C NH2
min0 5 10 15 20
mAU
0
20
40
60
80
100
120
140
UK036 (250 x 3mm, 0.3mL/min)
UK006 (250 x 4.6mm, 0.8mL/min)
UK806 (250 x 4.6mm, 0.8mL/min)
UKP06 (250 x 4.6mm, 0.8mL/min)
No.TI109E
TI109E-CA21
Unison, 250 x 4.6 mm20 mM H3PO4 0.6 mL/min, 37 °CUV at 210 nm
Organic Acids
1
2
3
4
6
5
7
8
succinic acid
(CH2)2HOOC COOH
COOHC
HC
HHOOC
fumaric acid
malonic acid
formic acid
lactic acid
citric acid
acetic acid
CH2COOHHOOC
COOHC
HC
H
HOOC
maleic acid
HCOOH
CH3COOH
CH3CHCOOHOH
HOCCH2COOHCOOH
CH2COOH
1
23
4
5
67
8
1
2
3
5,46
7
8
12
3
4
56 7
8
1 23
4
56 7
8
0 5 10 15 20 25min
0
100
200
mAU
UK-C18
UK-C8
UK-Phenyl
US-C18
HOCCH2COOHCOOH
CH2COOH
No.TI110E
TI110E-CA21
Organic Acids
150 x 4.6 mm20 mM H3PO4 1.0 mL/min, 37 °C, UV at 210 nm
1
2
3
4
6
5
7
8
succinic acid
(CH2)2HOOC COOH
COOHC
HC
HHOOC
fumaric acid
malonic acid
formic acid
lactic acid
citric acid
acetic acid
CH2COOHHOOC
COOHC
HC
H
HOOC
maleic acid
HCOOH
CH3COOH
CH3CHCOOHOH
12
3
4
5,67
8
1 23
4
56 7
8
0 2 4 6 8 10min
0
100
200
mAU
Brand - I
Rs(3/2)=1.6
Rs(3/2)=1.3
No.TI132E
TI132E-CF18�
Unison UK-C18, 250 x 4.6 mmwater / HFBA = 100 / 0.10.8 mL/min, ambientUV at 210 nm, 13 MPa100 µg ( 20 µL )
Urea
0 2 4 6 8 10min
0
20
40
mAU
ureaNH2H2N C
O
No.TI136E
TI136E-CG24
Unison, 250 x 4.6 mmwater / acetonitrile / TFA = 80/20/0.10.8 mL/min, 37 °C�210 nm, 10µg ( 5µL )
Caproic Acid Isomers
1
2
3
4
12
3 4
12
3 4
12,3
4
0
5
10
mAU
0 10 20 30 40 50min
0
10
20
mAU
0 10 20 30 40 50min
0
5
10
mAU
0 10 20 30 40 50min
OH
O
2-methylvaleric acid
3-methylvaleric acid
n-caproic acid
4-methylvaleric acid
OH
O
OH
O
OH
O
Rs 1.29
Rs 1.40
No.TI142E
TI142E-DA06
Unison, 75 x 4.6 mm1 mL/min, 37 °C, 260 nm2 µg (2µL)
Hydroquinone Isomers (Dihydroxybenzenes)
1 2 3
1
1
1
2
2
3
3
23
0 2 4 6 8 10min
0
100
200
mAU
hydroquinone resorcinol catechol(pyrocatechol)
HO OHHO
OH
OH
OH
Unison UK-C18water / acetonitrile = 95 / 5
Unison UK-C8water / acetonitrile = 98 / 2
Unison UK-Phenylwater
No.TI143E
TI143E-DA06
Unison, 75 x 4.6 mmwater / acetonitrile = 85 / 151 mL/min, 37 °C, 260 nm2 µg (2µL)
Methoxyphenol Isomers
1 2 3
1
1
1
2
2
3
3
2
3
p-methoxyphenol m-methoxyphenol
HO OCH3HO
OCH3
o-methoxyphenolOH
OCH3
Unison UK-C18
Unison UK-C8
Unison UK-Phenyl
0 2 4 6 8 10min
0
100
200
mAU
No.TI151E
TI151E-DC30
Unison UK-C8 water / acetonitrile / TFA = 80/20/0.10.5 mL/min, room temp., 210 nm, 10µg ( 5µL )
Caproic Acid Isomers
1 2 3 4OH
O
2-methylvaleric acid3-methylvaleric acid n-caproic acid4-methylvaleric acid
OH
O
OH
O
OH
O
500 x 4.6 mm
0 50 100 150min
0 20 40 60 min
Rs 1.40
250 x 4.6 mm
Rs 2.831
2
34
12
3 4
18.5 MPa8.9 MPa
No.TI158E
TI158E-DD21
EL Materials
Courtesy of Dr. Shigetoshi Yamada, Faculty of Engineering, Shizuoka Univ.
mA
U
0 5 10 15 20 min
0
50
100
CH3
CH3H3C
N N
N-phenyl-N,N',N'-tris(4-methylphenyl)-1,4-phenylenediamine
CF3F3C
N N
N,N'-bis(4-trifluoromethylphenyl)-N,N'-1,4-phenylenediamine
Cadenza CD-C18, 150 x 4.6 mm (CD005)water / methanol / acetic acid = 5 / 95 / 0.1�1 mL/min, 260 nm, 37°C, 17.5 µg
No.TI161E
TI161E-DE07
75 x 4.6 mm1 mL/min, 37 °C, 260 nm
Antioxidants
1 2 3
12 3
0 2 4 6 8 10min
1 2 3
1 2
3
Unison UK-Phenylwater / acetonitrile = 50 / 50
Unison UK-C8water / acetonitrile = 39 / 61
Unison UK-C18water / acetonitrile = 30 / 70
BHT (Butylhydroxytoluene)
CH3
CH3
CH3CH3
CH3H3C
H3C
OH
CC
TBHQ (Butylhydroquinone)
OH
CH3
CH3
CH3
OH
C
BHA (4-hydroxy-3-t-butylanisole)
OCH3
CH3
CH3
CH3
OH
C
unkn
own
unkn
own
No.TI170E
TI170E-DF09
L-Carnitine
1
1
Unison UK-Silica, 150 x 3 mmacetonitrile / 50mM ammonium acetate = 65 / 35, 0.5 mL/min (7MPa)37 °C, 210 nm, 2µL(20µg)
Unison UK-C18, 75 x 4.6 mmwater / heptafluoro-n-butyric acid = 100 / 0.1, 1 mL/min (7MPa)37 °C, 210 nm, 2µL(20µg)
L-carnitine
C C
H
OH
O
O-CH2 CH2N+H3C
CH3
CH3
Unison UK-Silica
0 2 4 6 8 10min
0
10
20
mAU
Unison UK-C18
0
10
20
mAU
0 2 4 6 8 10min
No.TI172E
TI172E-DF10
Unison UK-Silica, 250 x 2 mmTHF / acetic acid = 100 / 10.2 mL/min (7MPa), 37 °C, 260 nm1µL (2µg)
Acrylamide
acrylamide
OCH CH2C NH2
methacrylamide
OC CH2C NH2CH3
0 5 10 15min
0
50
100
mA
U
1
2
1 2
No.TI173E
TI173E-DF14
Cadenza CD-C18, 150 x 4.6 mmwater / acetonitrile / formic acid = 60 / 40 / 0.1
0.6 mL/min (7MPa), room temp.260 nm, 0.4µL (20µg)
Acetylsalicylic Acid, Salicylic Acid
min0 2 4 6 8 10
mAU
0
200
400
600
COOHOH
salicylic acid
O
COOHOCCH3
acetylsalicylic acid
No.TI187E
TI187E-DH31
Aromatic Sulfonates
Cadenza CD-C18, 50 x4.6mm10mM dihexylammonium acetate / tetrahydrofuran = 80 / 20
1mL/min, 37deg.C, 225nm, 20uL
CH3
SO O
ONa
SO O
ONa
SO O
ONa
SO O
ONa
CO
ONa
CH3
CH3
SO O
ONaSNaOO
O
SO
OONa
sodiump-toluenesulfonate
sodium xylenesulfonate
sodium benzoatesodium1-naphthalenesulfonate
disodium1,5-naphthalenedisulfonate
disodium2,6-naphthalenedisulfonate
0 2 4 6 8 min
0
10
20
mAU
sodi
um p
-tolu
enes
ulfo
nate
sodi
um x
ylen
esul
fona
te
sodi
um b
enzo
ate
sodi
um 1
-nap
htha
lene
sulfo
nate
diso
dium
1,5
-nap
htha
lene
disu
lfona
te
diso
dium
2,6
-nap
htha
lene
disu
lfona
te
No.TI190E
TI190E-DH27
Unison, 50 x 4.6 mmA: water / TFA = 100 / 0.1�B: acetonitrile / TFA = 100 / 0.1�0.5 - 50%B ( 0-5min )1 mL/min, 37 deg.C, 210 nm
Short-Chain Fatty Acids
0 2 4 6 8min
0
100
200
mAU
n-pr
opio
nic
acid
n-bu
tyric
aci
d
n-va
leric
aci
d
n-ca
proi
c ac
id
n-pr
opio
nic
acid
n-bu
tyric
aci
d
n-pr
opio
nic
acid
n-bu
tyric
aci
d
n-va
leric
aci
d
n-ca
proi
c ac
id
n-va
leric
aci
d
n-ca
proi
c ac
id OH
O
n-caproic acid
n-valeric acid
n-butyric acid
n-propionic acid
OH
O
OH
O
OH
O
No.TI191E
TI191E-DI01
Unison UK-Silica, 250 x 3 mmacetonitrile / 50mM ammonium formate = 70 / 300.5 mL/min (10MPa), 37 deg.C, 210 nm, 2uL(20ug)
Betaine
beta
ine
CO
O-CH2N+H3C
CH3
CH3
0 5 10 min
0
50
mAU
No.TI192E
TI192E-DI27
Unison UK-C18, 250 x 4.6 mmwater / triethylamine / acetic acid (100 / 0.2 / 0.1)0.8 mL/min (13MPa), 37 deg.C, 260 nm, 5 uL (2.5 ug)
UDP-SugarsUDP糖
0 10 20 30 min
100
mA
U
1 23
UDP-glucose
O
OH
OH
PO
HO
O OCH2
OH
PO
O
OHN
NOOHOCH2
HOOH
OH
UDP-arabinose
O
OH
OH
PO
HO
O OCH2
OH
PO
O
OHN
NOOHO
HOOH
OH
UDP-xylose
O
OH
OH
PO
HO
O OCH2
OH
PO
O
OHN
NOOHO
HOOH
OH
Courtesy of Prof. Shinichi Kitamura, Osaka Pref. Univ.
No.TI198E
TI198E-DJ08
Antioxidants
75 x 4.6 mmacetonitrile / tetrahydrofuran
= 80 / 201 mL/min, 37 deg.C, 230nm
Unison UK-Silica250 x 4.6 mm
hexane / ethyl acetate = 90 / 101 mL/min, 37 deg.C, 270nm
irgan
ox 1
010
irgaf
os 1
68
0 2 4 6 8 min
0
10
20
mA
U
(H3C)3C
(H3C)3C
C(CH3)3
C(CH3)3
C(CH3)3
C(CH3)3
PO
O
O
irgafos 168
OH
HO
HO
OH
C
O
O
OO
O
OO
O
C(CH3)3
C(CH3)3
C(CH3)3
C(CH3)3
C(CH3)3
(H3C)3C
(H3C)3C
(H3C)3C
irganox 1010
0 2 4 6 8 min
0
50
100
mA
U
irgan
ox 1
010
irgaf
os 1
68
No.TI204E
TI204E-DK24
ABEE-Converted Oligosaccharide
Courtesy of Dr. Akiko Yamashita and Dr. Hiroyuki Hashimoto, Shinshu Univ., Japan
Isomaltose
(Glc)
MaltotrioseMaltose
min
min
Nigerosylglucose
PanoseNigelose
Kojibiose
Kojibiosylglucose
Cadenza CD-C18, 75 x 4.6 mm0.1M ammonium acetate buffer (pH 4.0) / acetonitrile = 89 / 11 (v/v)0.5 mL/min, 20 deg.C, 305 nm
ABEE(Ethyl 4-aminobenzoate)
No.TI208E
TI208E-DL03
Unison UK-C18, 75 x 4.6 mmA: 10mM ammonium formateB: acetonitrile10 - 70%B (0-10min)1 mL/min (7MPa), 37 deg.C260 nm, 3 uL (0.1-0.7 uL/mL)
Anilines
NH2
aniline
NHCH3
N-methylaniline
N,N-dimethylaniline
NH3C CH3
0 5 10 min
100
mA
U
1
2
31
2
3
No.TI209E
TI209E-DL03
Unison UK-Silica, 150 x 3 mmhexane / ethanol = 50 / 500.5 mL/min (6MPa), 37 deg.C, 220 nmDMSO, 1uL/ml (1uL)
Dimethyl Sulfoxide (DMSO)
S
OCH3H3Cm
AU
0 5 10min
0
50
No.TI239E
TI239E-EF09
250 x 4.6 mmwater / trifluoroacetic acid = 100/0.10.8 mL/min, 37 deg.C, 220 nm, 5 uL (5mg)
Halo Acetic Acids
1
2
3
4
5
6
7
1
1
2
2
3,4
3
4
5
5
6,7
6,7
mA
U
0 5 10 15 20 min
0
100
monochloroacetic acid (MCAA)CH2ClCOOH
dichloroacetic acid (DCAA)CHCl2COOH
bromochloroacetic acid (BCAA)CHBrClCOOH
trichloroacetic acid (TCAA)CCl3COOH
monobromoacetic acid (MBAA)CH2BrCOOH
dibromoacetic acid (DBAA)CHBr2COOH
tribromoacetic acid (TBAA)CBr3COOH
No.TI240E
TI240E-EF09
Unison UK-Silica, 250 x 4.6 mmhexane / tetrahydrofuran / trifluoroacetic acid= 70 / 30 / 0.11 mL/min, 37 deg.C, 260 nm, 1 uL (2 - 4.5ug)
Pyromellitic acidsピロメリト酸
1 2
1
2
mA
U
0 5 10 min
0
200
O
O
O
O
OO
OCHO
HO
OC OH
OC
OC OH
pyromellitic acidpyromellitic dianhydride
No.TI257E
TI257E-FA06
Unison UK-C18, 250 x 4.6 mmwater / triethylamine / acetic acid =100 / 1.25 / 0.50.8 mL/min (14MPa), 37 deg.C, 220 nm, 1uL ( 2-35ug )
Causative Substances of Urolithiasis
1 2 3
1
2
3
mA
U
0 5 10 min
0
200
COOHHOOC
L-ascorbic acid
OHHO
CHOCH2
H OH
OO
oxalic acid
NH
HN
NH
HN
O
OO
Uric acid
No.TI261E
TI261E-DA17
EDTA-chelates
1 2 3
1
23
mA
U
0 2 4 6min
0
40
Fe (III)-EDTACu (II)-EDTA
N NCH2CH2ONa
O
CCH2
CH2
CH2
CH2 OHHO
NaO
O
C
O
C
O
C
EDTA disodium salt
Unison UK-Phenyl, 75 x 4.6 mm10mM tetrabutylammonium acetate + 20mM acetic acid+ 20mM ammonium acetate / acetonitrile = 90/10�1mL/min(5MPa), 37 degC, 210nm, 5uL(0.3-9.5ug)
O
O
O
O
O
OFe
N
O
O
N
-
Na+
O
O
O
O-
O
O-
N
N
O
OCu
2 -
2Na+
No.TI275E
TI275E-FG20
Cadenza CD-C18, 150 x 3 mmA: water /TFA = 100 /0.1B: acetonitrile /TFA = 100 /0.135-75%B (0-10 min)1 mL/min, 37 deg.C, 260 nm1 uL (0.2-0.6ug)
Aromatic Boronic Acids芳香族ボロン酸類
1
2
3
4
1
2
3 4
0 5 10 min
0
40
OHHOB
OHHOB
OHHOB
OHHOB
1,4-phenylenediboronic acid
benezeneboronic acid
1-naphthaleneboronic acid
2-naphthaleneboronic acid
B
OH
OH
No.TI283E
TI283E-FK28
250 x 4.6 mm0.6 mL/min, 37 deg.C260 nm, 1 ug (2 uL)
Phthalonitrile
terephthalonitrile (p-) phthalonitrile (o-)isophthalonitrile (m-)
C
C
N
NCC NN
C
C
N
N
1 2 3
1
2
3mA
U
0 5 10 15 20 25min
0
6
water / methanol= 65 / 3517 MPa
water / methanol= 75 / 2518 MPa
1 2 3
No.TI284E
TI284E-FK28
Dinitrobenzene
1 2 3
p-dinitrobenzene o-dinitrobenzenem-dinitrobenzene
NO2
O2NNO2O2N
NO2
NO2
1
1, 3
2
2
3
mA
U
water / methanol= 55 / 4519 MPa
water / methanol= 65 / 3519 MPa
0 10 20 30 40min
0
100
250 x 4.6 mm0.6 mL/min, 37 deg.C260 nm, 1 ug (2 uL)
No.TI285E
TI285E-FK28
Terphenyl related compounds
1
2
3
4
5250 x 4.6 mm0.8 mL/min, 37 deg.C260 nm, 50-320 ng (2 uL)
water / acetonitrile= 32 / 689 MPa
water / acetonitrile= 65 / 3517 MPa
1 2
3
4
5
1 2
3 4
5
mA
U
0 20 40min0
60
triphenylene
o-terphenyl
m-terphenyl
p-terphenyl
biphenyl
No.TI286E
TI286E-FK28
Polycyclic Aromatic Hydrocarbons (PAHs)
1
2
3
4
5
water / methanol= 10 / 9010 MPa
water / methanol= 30 / 7017 MPa
250 x 4.6 mm0.5 mL/min, 37 deg.C260 nm, 0.2-10 ug (1 uL)
1
2
3
4
5
1
2
3
4 5
mA
U
0 10 20 min
0
100
unkn
own
unkn
own
anthracene
biphenyl
propylbenzene
CH2CH2CH3
Acenaphthene
fluorene
No.TI298E
TI298E-GC07
Unison UK-C18, 150 x 4.6 mm10mM dibutylammonium acetate /acetonitrile = 90 /100.8 mL/min, 37 deg.C, 230 nm, 20uL
Anions
mA
U
0 2 4 6 8 10 min
0
20
40
SCN-NO3-
NO2-
I-
5ppm
500ppb
No.TI299E
TI299E-GC07
150 x 4.6 mm10mM dibutylammonium acetate /acetonitrile = 90 /100.8 mL/min, 37 deg.C, 230 nm, 5ppm, 20uL
Anions陰イオンの分析
mA
U
0 2 4 6 8 10 min
0
100
200
SCN-
SCN-
SCN-
SCN-
SCN-
NO3-
NO3-
NO3-
NO3-
NO3-
NO2-
NO2-
NO2-
NO2-
NO2-
I-
I-
I-
I-
I-
No.TI331E
TI331E-GH03
Fatty Acids
1. lauric acid (n=10)2. myristic acid (n=12)3. palmitic acid (n=14)4. stearic acid (n=16)5. arachidic acid (n=18)
normal phase
reversed phase
CH3(CH2)nCOOH
0 5 10 15 20 min
0
100
200mV
0 5 10 min
0
500mV
Cadenza CD-C18, 250 x 3 mmA: water /formic acid = 100 /0.1B: acetonitrile / THF / formic acid = 50 / 50 / 0.175-95%B (0-20min)0.4mL/min (12 MPa), 37deg.CELSD, 0.7uL (0.8-4ug)
Unison UK-Amino, 250 x 3 mmacetonitrile /10mM ammonium acetate = 90 /100.4mL/min (6 MPa), 50deg.CELSD, 0.5uL (0.6-3ug)
12
3
4
5
1
23
4
5
No.TI343E
TI343E-GG30
Unison UK-Amino250 x 4.6 mm,A: hexane, B: ethanol0.2-10%B(0-50min)1 mL/min 37 deg.C260 nm, 5 uL (10% solution)
Cadenza CL-C18250 x 4.6 mmwater / acetonitrile = 40 / 600.8 mL/min, 37 deg.C260 nm, 5 uL (10% solution)
Surfactant (triton)
C8H17 OCH2CH2 nOH
n = ~10Triton X-100
mA
U
0 20 40 60 min
0
100
mA
U
0 20 40 60 min
0
100
normal phase
reversed phase
No.TI344E
TI344E-GG30
Surfactant (Igepal)
C9H19 OCH2CH2 nOH
n = ~12Igepal CO-720
mA
U
0 20 40 60 min
0
40
mA
U
0 20 40 60 min
0
40
Unison UK-Amino250 x 4.6 mm,A: hexane, B: ethanol0.2-10%B(0-50min)1 mL/min 37 deg.C260 nm, 5 uL (10% solution)
Cadenza CL-C18250 x 4.6 mmwater / acetonitrile = 40 / 600.8 mL/min, 37 deg.C260 nm, 5 uL (10% solution)
normal phase
reversed phase
No.TI348E
TI348E-GH06
250 x 4.6 mmwater / trifluoroacetic acid = 100 / 0.10.6 mL/min, 37 deg.C, 210 nm, 1.4uL (0.01-7ug)
Organic Acids
0
0
8succinic acid
(CH2)2HOOC COOH
3malonic acid
CH2COOHHOOC
6COOH
CH
CH
HOOC
maleic acid
2formic acid
HCOOH
5acetic acid
CH3COOH
4lactic acid
CH3CHCOOHOH
7
citric acid
HOCCH2COOHCOOH
CH2COOH
1 HOOCCHCHCOOHOH
OHtartaric acid
COOHC
HC
HHOOC
fumaric acid
9
CH3CH2COOHpropionic acid
10
1
2
34
5
6
7 8
9 10
1
2
34
5
6
7 8
9 10
50
100
10 20 30 min
mAU
No.TI380E
TI380E-HA22
Polyethylene glycol # 2000
Unison UK-Amino, 250 x 4.6 mmA: hexane, B: ethanol 7-11%B(0-50min)1mL/min (6MPa), 37deg.C, ELSD2uL (40ug)
Cadenza CW-C18, 250 x 4.6 mmA: water, B: acetonitrile25-30%B(0-46min)1mL/min (18MPa), 37deg.C, ELSD2uL (100ug)
reversed phase
normal phase
0 20 40 60 min
0
20
40mV
0 20 40 min
0
20
40mV
60
polyethylene glycol #2000 (Av. Mw. 2000)
OCH2CH2 nOHH
No.TI381E
TI381E-HA22
Polyethylene glycol # 2000, 6000, 12000
Unison UK-Amino, 150 x 4.6 mmA: hexane, B: tetrahydrofuran70-75%B(0-8min)1mL/min (4MPa), 60deg.C, ELSD0.4uL (3ug)
Cadenza CW-C18, 150 x 4.6 mmA: water, B: tetrahydrofuran10-15%B(0-10min)1mL/min (14MPa), 37deg.C, ELSD0.4uL (7ug)
reversed phase
1
2
3
1
2 3
normal phase
OCH2CH2 nOHH
0
40mV
0 5 10 min
0
100mV
0 5 10 min
1 polyethylene glycol #2000 (Av. Mw. 2000)2 polyethylene glycol #6000 (Av. Mw. 8500)3 polyethylene glycol #12000 (Av. Mw. 12000)
No.TI383E
TI383E-HC07
Halo Acetic Acids
reversed phase
normal phase
12
3
4
Unison UK-Amino, 250 x 3 mmacetonitrile /50mM TFA-ammonium= 20 /800.4 mL/min (13 MPa), 37 deg.C220 nm, 5 uL (40-400 ug)
Cadenza CW-C18, 250 x 3 mmwater /TFA = 100 /0.070.4 mL/min (14 MPa), 37 deg.C220 nm, 5 uL (40-400 ug)
1monochloroacetic acid (MCAA)CH2ClCOOH
4bromochloroacetic acid (BCAA)CHBrClCOOH
6trichloroacetic acid (TCAA)CCl3COOH
5dibromoacetic acid (DBAA)CHBr2COOH
7tribromoacetic acid (TBAA)CBr3COOH
0
20
mV
0 10 20 min
0
20mV
0 10 min5 15
5
6
7
2monobromoacetic acid (MBAA)CH2BrCOOH
3dichloroacetic acid (DCAA)CHCl2COOH
12
3
4
5
6
7
No.TI403E
TI403E-HD08
Cadenza CD-C1850 x 2 mmA: water / MeOH / TFA = 70 / 30 / 0.1B: ACN / TFA = 100 / 0.130-60 %B (0-0.8min)60-100 %B (0.8-1.5min)0.8 mL/min (17MPa)37 deg.C, 280 nm1 uL (0.1-0.2ug)
Cadenza 5CD-C18150 x 4.6 mmA: water / MeOH / TFA = 70 / 30 / 0.1B: ACN / TFA = 100 / 0.130-60 %B (0-8min)60-100 %B (8-15min)1 mL/min (6 MPa)37 deg.C, 280 nm1 uL (0.1-0.2ug)
Antioxidants
1 2 3 4
5 6 7 8
1
2 34
5
67
8
mA
U
0 5 10 min
0
25
1
2 3
4
5
6
78
mA
U
0 0.5 1.0 min
0
50
4-hydroxy-3-t-butylanisolebutylhydroquinone
butylhydroxytolueneCH3
CH3
CH3CH3
CH3H3C
H3C
OH
CC
CH2OH
CH3
CH3CH3
CH3H3C
H3C
OH
CC
OH
CH3
CH3
CH3
OH
C
OCH3
CH3
CH3
CH3
OH
C
OH
OHHO
O O(CH2)2CH3
n-propyl gallate
OH
OHHO
O O(CH2)7CH3
n-octyl gallate
OH
OHHO
O O(CH2)11CH3
n-dodecyl gallate
OH
OH
HO
HO
CH3
CH3
nordihydroguaiaretic acid
2,6-di-tert-butyl-4-hydroxymethylphenol
No.TI437E
TI437E-HI26
Unison UK-Amino, 150 x 2 mmA: acetonitrileB: 10mM ammonium formate10-35%B(0-10min), 0.2 mL/min (4MPa), 37 deg.CELSD, 2 uL(1ug, 2.5%NH4OH)
Melamine and Cyanuric acid
N
NN
OH
OHHO
cyanuric acid
melamine
N
NN
NH2
NH2H2N
1
2
12
0 5 10 min
0
20
40mV
No.TI438E
TI438E-HJ03
Unison UK-Amino, 150 x 3 mmA: acetonitrile, B: 10mM ammonium acetate + 0.1% acetic acid25%B (Isocratic)0.4 mL/min (5.9MPa), 40 deg.C, 5uL
LC-MS/MS: Melamine and Related Compounds
N
NN
OH
OHHO
3. cyanuric acid1. melamine
N
NN
NH2
NH2H2N N
NN
NH2
NH2HO
2. ammeline
N
NN
OH
HO NH2
4. ammelide
1
2
3
4
Positive Negative100 ppb
600 ppb
200 ppb
30 ppb
No.TI451E
TI451E-HK07
Acrylic Monomers
reversed phase
1
2 3 1
2
3
12
3
normal phaseUnison UK-Amino, 150 x 3 mmhexane / ethanol / TFA = A / B / 0.10.4 mL/min (3-4MPa), 37 deg.C, 0.2-0.4 uL(1.2-3.6ug, dissolved with eluent)
Unison UK-Phenyl, 250 x 3 mmwater / methanol / TFA = 100 / 0.5 / 0.1 0.4 mL/min (12 MPa), 37 deg.C, 215 nm0.4 uL (0.1-0.4ug)
0 5 10
0
200mV
min
acrylamide
acrolein
acrylonitrile
OCH CH2C NH2
OCH CHH2C
CHH2C C N
A / B = 99 /1215 nm
A / B = 80 /20230 nm
0 10 min
0
50
100mV
155
No.TI452E
TI452E-HJ31
Acrylamide and Methacrylamide
reversed phase
1
2
1
2
12
normal phase
Unison UK-Amino, 150 x 3 mmTHF /water /acetic acid = 100 /0.8 / 0.10.4 mL/min (5MPa), 37 deg.C, 230nm 1 uL (0.3-0.4ug, dissolved with eluent)
Cadenza CL-C18, 250 x 3 mmwater /acetonitrile /TFA = 97 /3 /0.10.4 mL/min (12 MPa), 45 deg.C, 230 nm1 uL (0.3-0.4ug)
acrylamide
methacrylamide
0 2 4 6 min0
50
100mV
0 5 10 min
0
20
40
60mV
OCH CH2C NH2
OC CH2C NH2CH3
No.TI453E
TI453E-HK07
Acrylic Monomers
1
2
3
4
2,3
4
2,34
1
2
3
4
250 x 3 mmwater / methanol / TFA = 100 / 0.5 / 0.10.4 mL/min (12-14MPa), 37 deg.C, 215 nm0.4 uL (0.1-0.3ug)
acrylamide
OCH CH2C NH2
methacrylamide
OC CH2C NH2CH3
OCH CHH2C
acrolein
CHH2C C N
acrylonitrile
1
1
0 10 200
50
100
mV150
min
No.TI456E
TI456E-HK25
75 x 3 mmA: water /formic acid = 100 /0.1B: acetonitrile /formic acid = 100 /0.130 - 70 %B (0-10 min)0.4 mL/min (5 MPa)37 deg.C, 260 nm1 uL (100 ng)
Malachite Green and Metabolite
1 2
1
1
1
1
1
2
2
2
2
2
mA
U
0 5 10 min
0
60
malachite green
N N+
leucomalachite green
N N
No.TI457E
TI457E-HK25
75 x 4.6 mmA: water / formic acid = 100 / 0.1B: acetone / formic acid = 100 / 0.160 - 90 %B (0-10 min)1 mL/min (7-8 MPa)37 deg.C, 500 nm2 uL (40 ng)
Sudan Dyes
1 2 3 4
sudan I
N N
HO
sudan III
N NN N
HO
sudan II
N N
HO
sudan IV
N NN N
HO
1
1
2
3
2
3
4
4
1 2
3
4
12
3
4
1 2
3
4
1 2
3
4
mA
U
0 5 10 min0
120
No.TI458E
TI458E-HL05
Unison UK-C18, 75 x 3 mmA: water / HFBA = 100 / 0.1B: acetonitrile / HFBA = 100 / 0.120-40%B (0-7min)0.4 mL/min (7 MPa), 37 deg.C, ELSD0.8 uL (1-2 nL)
Aliphatic amines
0 5 10
0
min
100
200
300mV
ethylenediamine
NH2H2N
diethylenetriamine
NH
H2N NH2
triethylenetetramine(including branched structures)
NH
H2N NH2HN
tetraethylenepentamine(including branched structures)
NH
H2NHN N
HNH2
1
2
3
4
1
2
3
4
No.TI482E
TI482E-IB18
Unison UK-Amino, 150 x 2 mmacetonitrile / water / acetic acid = 98 / 2 / 10.2mL/min (3MPa), 37 deg.C280 nm, 0.6 uL (0.3-3ug)
Unison UK-Silica, 150 x 2 mmA: hexane / formic acid = 100 / 0.5B: tetrahydrofuran / formic acid = 100 / 0.52-15 %B (0-10min)0.2mL/min (2MPa), 37 deg.C280 nm, 1 uL (0.5-2.5ug)
Food preservatives (normal phase)
1 2 3COOH
benzoic acidpotassium sorbatesodium dehydroacetate
O
OKO
O
ONa
OCH3
H3C
1
2
3
mA
U
0 5 10min
0
200
12
3
mA
U
0 5 10 min
0
100
No.TI483E
TI483E-IB20
Denatonium benzoate
1 2
denatonium benzoate
OO- H
N
ON+
1
2
12
1
1
2
2
mA
U
0 1 2 3 4 min
0
100
50 x 3 mmA: water / TFA = 100 / 0.1, B: acetonitrile / TFA = 100 / 0.120-50 %B (0-3min), 0.43 mL/min (3-4MPa)37 deg.C, 225 nm, 1 uL (100ppm)
No.TI484E
TI484E-IB20
Denatonium benzoate
1 2
denatonium benzoate
OO- H
N
ON+
Cadenza CD-C18, 50 x 3 mmwater / acetonitrile / TFA = 65 / 35 / 0.10.3 mL/min (2MPa), 37 deg.C, 225 nm
1
2
mA
U
0 2 4 min
0
10
20
5 ppm x 2 uL
10 ppm x 1 uL
25 ppm x 0.4 uL
50 ppm x 0.2 uL
0 5 10 150
200
400
mV
min
Na+
1
2
3
4 5
6
7 89
Na+
1,2
3
4,6,9
5
78
150 x 3 mmA: 40mM formic acid / 40mM ammonium formate / acetonitrile = 80 / 20 / 1B: 80mM formic acid / 80mM ammonium formate / acetonitrile = 56 / 14 / 300 - 100 %B (0-15min), 0.4 mL/min(8-9 MPa), 37 deg.CELSD (spray chamber 17deg.C, drift tube 42deg.C), 4 uL (1.6-22 ug)
COOH
CHOglyoxylic acid
succinic acid(CH2)2HOOC COOH
COOHC
HC
HHOOC
fumaric acid
malonic acid
lactic acid
citric acid
CH2COOHHOOC
COOHC
HC
H
HOOC
maleic acid
CH3CHCOOHOH
OHCHCH2COOHCOOH
CH2COOH
sodium gluconate
OH
OHONa
OH
CH2OHO
HO
malic acidCH2 CH OHHO CC
O OOH
1
2
3
4
5
6
7
8
9
No.TI518E
TI518E-IF03
Organic acids
Imtakt Corp./JAPAN ([email protected])Imtakt USA ([email protected])North America Other Countries
0
Cl-
Na+
0%B
5%B
10%B
Scherzo SM-C18, 150 x 3 mmA: 5mM ammonium formate, B: acetonitrile0.4 mL/min (9-10 MPa), 37 deg.CELSD (spray chamber 20 deg.C, drift tube 45 deg.C)1.6 uL (0.16ug NaCl)
0 5 10
100
200
mV
min
300
No.TI520E
TI520E-IF05
Sodium chloride (NaCl)
Imtakt Corp./JAPAN ([email protected])Imtakt USA ([email protected])North America Other Countries
0 5 10
0
50
100
mA
U
1
23
1,2
3
min
150 x 3 mm100mM NH4H2PO4 / acetonitrile = 80 / 200.4 mL/min (9-10MPa), 37 deg.C, 210 nm20 uL
CH3COOHacetic acid (100ppm)
1
CF3COOHtrifluoroacetic acid (100ppm)
2
CCl3COOHtrichloroacetic acid (9ppm)
3
No.TI523E
TI523E-IF22
Acetic acid and trihaloacetic acids
Imtakt Corp./JAPAN ([email protected])Imtakt USA ([email protected])North America Other Countries
OH
O
OHH3C
O
OHO
H3C
OH
O
OCH3H3C
O
OCH3O
H3C
lactic acid
levulinic acid
methyl lactate
methyl levulinate
1
2
3
4
150 x 4.6 mm5 mM NH4H2PO4 / acetonitrile = 93 / 71 mL/min (11 MPa), 37 deg.C, 210 nm, 10 uL (13ug)
0 5 10
0
50
100
mA
U
150
min
1
2
3
42
3
1
4
No.TI530E
TI530E-IG15
Levulinic acid, lactic acid and methyl esters
Imtakt Corp./JAPAN ([email protected])Imtakt USA ([email protected])North America Other Countries
0 10 20 min0
100
mA
U
0 20 40 min
0
100 anthracene
Acenaphthene
fluorene
naphthalene
benzo(j)fluoranthene
benzo(e)pyrene
No.TI538E
TI538E-IH17
Cadenza CL-C18, 250 x 3 mm0.4 mL/min, 37 deg.C, 260 nm1.4 uL (0.05-0.7 ug)
Polycyclic aromatic hydrocarbons (PAHs)
Imtakt Corp./JAPAN ([email protected])Imtakt USA ([email protected])North America Other Countries
1
2
34
56
1
34
2
5 6
1
2
3
4
5
6
water / acetonitrile = 12 / 889MPa
water / methanol = 12 / 8817MPa
No.TI542E
TI542E-II11
Unison UK-Amino, 250 x 3 mmA: water / acetonitrile / formic acid = 30 / 70 / 0.1, B: water / formic acid = 100 / 210-60 %B (0-25min), 100 %B (25-60min)0.4 mL/min (11MPa), 37 deg.C, ELSD (spray chamber 19 deg.C, drift tube 42 deg.C)10 uL (2.3-14 ug, diluted with 2% formic acid)
Organic acids
Imtakt Corp./JAPAN ([email protected])Imtakt USA ([email protected])North America Other Countries
0 20 40 60 min0
100
200mV
(CH2)2HOOC COOH
COOHC
HC
HHOOCCH2COOHHOOC
COOHC
HC
H
HOOC
CH3CHCOOHOH
C COOHCH2COOH
CH2COOHHO
OH
OHONa
OH
CH2OHO
HO
CH2 CH OHHO CCO OOH COOH
CHO
CH CH OHHO CCO
OOH
OH
succinic acid
fumaric acidmalonic acid
lactic acid
citric acid
maleic acid
sodium gluconate
malic acid glyoxylic acid
tartaric acid
1
2
3
4
56
7
8
9
10
Na+
1 2 3
4 5
6 7
8 9
10
No.TI546E
TI546E-IJ15
150 x 3 mmA: water / formic acid = 100 / 0.1, B: acetonitrile / formic acid = 100 / 220-70 %B (0-10min), 0.4 mL/min (9MPa)37 deg.C, 275 nm, 2 uL (0.2-1 ug)
Aromatic carboxylic acids
Imtakt Corp./JAPAN ([email protected])Imtakt USA ([email protected])North America Other Countries
1,6
1
2
2
3
3
4
4
5
5
6 7
7
mA
U
0 5 10 min
0
100
200
4
isophthalic acid (m-)(pKa=3.7, 4.6)
COOH
HOOC
3
terephthalic acid (p-)(pKa=3.5, 4.4)
COOHHOOC
6
o-phthalic acid(pKa=2.7, 4.9)
COOH
COOH
2
m-hydroxybenzoic acid(pKa=4.3)
OH
HOOC
1
p-hydroxybenzoic acid(pKa=4.5)
OHHOOC
7
o-hydroxybenzoic acid(pKa=2.9, 13.6)
OH
COOH
5
homophthalic acid
COOH
CH2COOH
No.TI547E
TI547E-IK10
Unison UK-Phenyl75 x 3 mmA: water / acetic acid = 100 / 0.1B: methanol / acetic acid = 100 / 0.170-90 %B (0-8min)70 %B (8-10min)0.4 mL/min (7MPa)37 deg.C, 275 nm2 uL (0.1-0.5ug)
Cadenza CW-C1875 x 3 mmA: water / acetic acid = 100 / 0.1B: acetonitrile / acetic acid = 100 / 0.170-100 %B (0-8min)70 %B (8-10min)0.4 mL/min (4MPa)37 deg.C, 275 nm2 uL (0.1-0.5ug)
Phthalate esters
1
2
3
4
5
6
Imtakt Corp./JAPAN ([email protected])Imtakt USA ([email protected])North America Other Countries
1
2
3
4 56
mA
U
0 2 4 6 8 10 min
0
20
12
3
4 56
mA
U
0 2 4 6 8 10 min
0
20
O
OOO
O
OOO
O
OOO
butylbenzyl phthalateBBP
dibutyl phthalateDBP
di-2-ethylhexyl phthalateDEHP
di-n-octyl phthalateDNOP
diisononyl phthalateDINP
O
OOO
O
OOO
C9H19C9H19
diisodecyl phthalateDIDP
O
OOO
C10H21C10H21
Imtakt Corp./JAPAN ([email protected])Imtakt USA ([email protected])North America Other Countries
250 x 3 mmA: water / formic acid = 100 / 0.3, B: acetonitrile / formic acid = 100 / 2 0-5 %B (0-13min), 50 %B (13-30min)0.4 mL/min (13MPa), 37 deg.CELSD (spray chamber 19deg.C, drift tube 43deg.C), 4 uL (3.8-6.2 ug)
CH2 CH OHHO CCO OOH
malic acid
CH CH OHHO CCO
OOH
OHtartaric acid
(CH2)2HOOC COOHsuccinic acid
CH2COOHHOOCmalonic acid
C COOHCH2COOH
CH2COOHHO
citric acid
COOHC
HC
HHOOC
fumaric acid
COOHC
HC
H
HOOC
maleic acid
No.TI548E
Organic acids (2)
TI548E-IJ29
1
2
3
4
5
6
7
1
0 10 20 30 min0
100
mV150
50
2
3
4
56
7
12 3,645
7
No.TI549E
TI549E-IJ29
Sodium thiosulfate
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S2O32-
Na+
Na2S2O3sodium thiosulfate
A: 50 mM acetic acidB: 100 mM ammonium acetate0-100 %B (0-3min), 100 %B (3-10min)8MPa
0 5 10
0
100
200
mV
min
A: 50 mM acetic acid /acetonitrile = 90 /10B: 100 mM ammonium acetate /acetonitrile = 90 /100-100 %B (0-3min), 100 %B (3-10min)9MPa
Scherzo SM-C18, 150 x 3 mm0.4 mL/min, 37 deg.C,ELSD (spray chamber 50 deg.C, drift tube 100 deg.C)1.6 uL (16 ug)
No.TI551E
TI551E-IK20
Scherzo SM-C18, 50 x 3 mm0.4 mL/min (5MPa), 37 deg.C, ELSD (spray chamber 50deg.C, drift tube 100deg.C), 3 uL (10-20ug)
Chromium (III) nitrate and ammonium chromate(VI)
Imtakt Corp./JAPAN ([email protected])Imtakt USA ([email protected])North America Other Countries
0 5 10
0
20
40
mV
min
A: 25 mM ammonium formateB: 100 mM formic acid0 %B (0-3min), 0-100 %B (3-6min), 100 %B (6-10min)
A: 25 mM ammonium formate /acetonitrile = 90 /10B: 100 mM formic acid /acetonitrile = 90 /100 %B (0-3min), 0-100 %B (3-6min), 100 %B (6-10min)
Cr(NO3)3.9H2Ochromium (III) nitrate nonahydrate
(NH4)2CrO4ammonium chromate(VI)
NO3-
Cr3+
CrO42-
O As ONa
O
AsH3C OH
OH
O
AsH3C OH
CH3
O
AsHO ONa
ONa .7H2O
sodium arsenite
dimethylarsinic acid(DMAA)
methylarsonic acid(MAA)
disodium hydrogenarsenateheptahydrate
No.TI552E
TI552E-IK20
250 x 3 mmA: water / formic acid = 100 / 0.01, B: water / formic acid = 100 / 10 %B (0-1.5min), 0-60 %B (01.5-15min)0.4 mL/min (13MPa), 37 deg.CELSD (spray chamber 50deg.C, drift tube 100deg.C), 5 uL (6-22ug)
Arsenic compounds
Imtakt Corp./JAPAN ([email protected])Imtakt USA ([email protected])North America Other Countries
0 5 10 15 20
0
50
100mV
min
1
2
3
4
1
23
4
Na+
1,4
23
Na+
Scherzo SM-C18
Unison UK-C18
No.TI553E
TI553E-IL18
Presto FF-C18250 x 4.6 mmwater / acetonitrile = 40 / 600.45 mL/min (28MPa)37 deg.C, 230 nm, 3 uL (3ug)
Cadenza CD-C18250 x 4.6 mmwater / acetonitrile = 10 / 901 mL/min (9MPa)37 deg.C, 230 nm, 3 uL (3ug)
Diisononyl phthalate
Imtakt Corp./JAPAN ([email protected])Imtakt USA ([email protected])North America Other Countries
diisononyl phthalateDINPO
OOO
C9H19C9H19
mA
U
0 20 40 min
0
10
mA
U
0 20 40 min
0
20
No.TI571E
TI571E-JB20
Polyethylene glycols (PEG)
Imtakt Corp./JAPAN ([email protected])Imtakt USA ([email protected])North America Other Countries
0 5 10 15min
0
20
40
mV
Presto FF-C18, 150 x 4.6mmA: waterB: acetonitrile30-40 %B (0-2min), 40-60 %B (2-15min)0.4 mL/min (24MPa), 37 deg.CELSD (spray chamber 50deg.C, drift tube 100deg.C), 3 uL (1.5-6ug)
1
2
3
4
OCH2CH2 nOHH 1 polyethylene glycol (ca. 2,000 Da)2 polyethylene glycol (ca. 8,500 Da)3 polyethylene glycol (ca. 12,000 Da)4 polyethylene glycol (ca. 20,000 Da)
No.TI572E
TI572E-JB12
Polyvinyl alcohol (PVA)
Imtakt Corp./JAPAN ([email protected])Imtakt USA ([email protected])North America Other Countries
0 5 10 min
0
20
40
mV
Presto FF-C18, 150 x 4.6mmA: waterB: acetonitrile5-90 %B (0-10min), 0.4 mL/min (22MPa), 37 deg.CELSD (spray chamber 50deg.C, drift tube 100deg.C), 2 uL (10ug)
polyvinyl alcohol
CHH2C
nOH
No.TI573 E
TI573E-JA15
Presto FF-C18, 150 x 4.6 mmA: waterB: acetonitrile0-100 %B (0-10min), 100 %B (10-15min)0.4 mL/min (15MPa), 50 deg.C, ELSD (spray chamber 50 deg.C, drift tube 100 deg.C)1 uL (5 ug, methanol)
Polyvinyl acetate
Imtakt Corp./JAPAN ([email protected])Imtakt USA ([email protected])North America Other Countries
0 5 10 15 20 min
0
50
100mV
polyvinyl acetate (120 ~ 140 kDa)
CHH2C
n
OOC
CH3
No.TI574E
TI574E-JA15
Polystyrene
Imtakt Corp./JAPAN ([email protected])Imtakt USA ([email protected])North America Other Countries
0 5 10 15 20min
0
100
mV
Presto FF-C18, 150 x 4.6mmA: acetonitrileB: tetrahydrofuran20-90 %B (0-15min), 0.4 mL/min (9MPa), 37 deg.CELSD (spray chamber 50deg.C, drift tube 100deg.C)0.6 uL (3 ug, tetrahydrofuran)
polystyrene (ca.200 kDa)
CHH2C
n
No.TI575E
TI575E-JA18
Presto FF-C18, 150 x 4.6 mmA: water / formic acid = 100 / 0.1B: tetrahydrofuran0-100 %B (0-10min), 100 %B (10-15min)0.4 mL/min (17MPa), 37 deg.CELSD (spray chamber 50 deg.C, drift tube 100 deg.C)0.4 uL (0.4 ug, tetrahydrofuran)
Poly DL-Lactide
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0 5 10 15 20 min
0
200
400mV
poly DL-lactide(75 ~120 kDa)
O
O
nCH3
No.TI578E
TI578E-IJ26
Presto FF-C18, 30 x 4.6 mmA: water, B: acetonitrile, 0-90 %B (0-1.5min)1 mL/min (9MPa), 37 deg.C, 260 nm, 0.4 uL
Surfactants
1
1
2
2
mA
U
Imtakt Corp./JAPAN ([email protected])Imtakt USA ([email protected])North America Other Countries
C8H17 OCH2CH2 nOH
n = ~10
Triton X-100
C9H19 OCH2CH2 nOH
n = ~12
Igepal CO-720
0 1 2 3 min
0
200
400
No.TI601E
TI601E-LD08
Urea and related compounds
1 2 3 4
Imtakt Corp./JAPAN ([email protected])Imtakt USA ([email protected])North America Other Countries
1
2
3
4
0 5 10 15 min
0
20
40
mV
1
23 4
mA
U
0 2 4 6 8 10 min
0
50
HN NH2
S
N-methylthiourea
Unison UK-Amino, 250 x 4.6 mmacetonitrile / water / formic acid = 95 / 5 / 0.11 mL/min (6MPa), 37 deg.CELSD (spray chamber 15 deg.C, drift tube 50 deg.C)4 uL (1-4 ug)
Unison UK-Amino, 250 x 4.6 mmacetonitrile / 100 mM phosphoric acid = 90 / 101 mL/min (6MPa), 37 deg.C210 nm, 2 uL (0.06-70 ug)
NH2H2NC
O
urea
NH2NC
O
H
HO
3-hydroxyphenylurea
NH2NC
O
H
HO
hydroxyurea
No.TI602E
TI602E-JD13
Biodegradable polymer ( poly (DL-lactide) and poly (DL-lactide-co-glycolide) )
Imtakt Corp./JAPAN ([email protected])Imtakt USA ([email protected])North America Other Countries
Cadenza CW-C18, 150 x 3 mmA: water / formic acid = 100 / 0.1B: acetone / ethyl acetate / formic acid = 70 /30 / 0.10-100 %B (0-15min), 0.6 mL/min (11MPa), 60 deg.CELSD (spray chamber 45 deg.C, drift tube 100 deg.C), 1 uL (10ug, THF)
poly (DL-lactide)
O
O
nCH3
poly (DL-lactide-co-glycolide)n
O
O
CH3O
O
m X
lactide / glycolide = 75 / 25 (mole %)
lactide / glycolide = 50 / 50 (mole %)
ca. 20 kDa
ca. 15 kDa
ca. 10 kDa
ca. 5 kDa
0 5 10 15 20 min
0
100
200mV
0 5 10 15 20 0 5 10 15 20
No.TI603E
TI603E-JD13Imtakt Corp./JAPAN ([email protected])Imtakt USA ([email protected])North America Other Countries
poly (DL-lactide)
O
O
nCH3
poly (DL-lactide-co-glycolide)n
O
O
CH3O
O
m X
lactide / glycolide = 75 / 25 (mole %) lactide / glycolide
= 50 / 50 (mole %)
Unison UK-Amino , 150 x 3 mmA: hexane / acetic acid = 100 / 0.1B: acetone / acetic acid = 100 / 0.130-100 %B (0-10min), 100 %B (10-15min)0.6 mL/min (4MPa), 60 deg.CELSD (spray chamber 50 deg.C, drift tube 100 deg.C), 0.6 uL (6 ug, THF)
ca. 20 kDa
ca. 15 kDa
ca. 10 kDa
ca. 5 kDa
Biodegradable polymer ( poly (DL-lactide) and poly (DL-lactide-co-glycolide) )
0
100
mV
0 5 10 15 20 0 5 10 15 20 min0 5 10 15 20
No.TI604E
TI604E-JD13Imtakt Corp./JAPAN ([email protected])Imtakt USA ([email protected])North America Other Countries
Biodegradable polymer ( poly (DL-lactide) and poly (DL-lactide-co-glycolide) )
poly (DL-lactide)
O
O
nCH3
poly (DL-lactide-co-glycolide)n
O
O
CH3O
O
m X
lactide / glycolide = 75 / 25 (mole %)
lactide / glycolide = 50 / 50 (mole %)
Presto FF-C18, 30 x 4.6 mmA: water / formic acid = 100 / 0.1B: acetone / ethyl acetate / formic acid = 70 / 30 / 0.10-100 %B (0-1.5min), 100 %B (1.5-3min)0.6 mL/min (4 MPa), 60 deg.CELSD (spray chamber 45 deg.C, drift tube 100 deg.C), 0.4 uL (4 ug, THF)
ca. 20 kDa
ca. 15 kDa
ca. 10 kDa
ca. 5 kDa0
200mV
0 2 4 0 2 4 min40 2
No.TI607E
TI607E-JE13
Organic acids (Anion exchange mode)
1 2 3 4 5
Imtakt Corp./JAPAN ([email protected])Imtakt USA ([email protected])North America Other Countries
Unison UK-Amino, 250 x 4.6 mmA: 50mM NH4H2PO4B: 50mM NH4H2PO4 : 50mM (NH4)2HPO4 = 1 : 10-50 %B (0-15min)1 mL/min (18MPa), room temperature, 210 nm5 uL (7.5 ug)
1
2
3
4
5
mA
U
0 5 10 15 min
0
50
100
sodium gluconate
OH
OHONa
OH
CH2OHO
HO
COOH
CHO
glyoxylic acid formic acid
HCOOH
acetic acid
CH3COOH
lactic acid
CH3CHCOOHOH
No.TI609E
TI609E-JE28
Cations
Imtakt Corp./JAPAN ([email protected])Imtakt USA ([email protected])North America Other Countries
Scherzo SM-C18, 150 x 3 mmA: 5 mM ammonium formateB: 100 mM formic acid 0-50 %B (0-15 min)0.4 mL/min (10 MPa), 37 deg.CELSD (spray chamber 50 deg.C, drift tube 100 deg.C)0.6 uL ( 0.7-2.4 ug)
Zn2+
Cu2+
Cr3+
0 5 10 150
100
mV
min
Na+
K+
Ca2+
Mg2+200
300
mA
U
0 1
0
50
100
min2 3
N O
SZn
NO
S
N O
SCu
NO
S
zinc pyrithione
copper pyrithione
No.TI610E
TI610E-JE28
Zinc pyrithione, copper pyrithione
Imtakt Corp./JAPAN ([email protected])Imtakt USA ([email protected])North America Other Countries
Unison UK-Phenyl, 50 x 3 mmA: 5mM ammonium hydrogencarbonateB: tetrahydrofuran60-95 %B (0-3 min)0.4 mL/min (7 MPa), 37 deg.C, 330 nm0.2 uL (0.1 ug, tetrahydrofuran)
1
2
1
2
No.TI617E
TI617E-JI09
Coumarins1
2
3
4
5
6
7
8
Imtakt Corp./JAPAN ([email protected])Imtakt USA ([email protected])North America Other Countries
1 2
1
1
2
2
1 2
1 2
12
1 2
34
34
3
3
4
4
34
34
3,4
5
5
6
6
5
5
5
5
5
6
6
6
6
6
7
7
7
7
7
7
7
8
8
8
8
8
8
8mA
U
0 10 20 30 min
0
100
200
150 x 4.6 mmA: water / acetic acid = 100 / 1B: acetonitrile / acetic acid = 100 / 11 mL/min (9-11MPa), 37 deg.C, 270 nm2 uL (0.2-1.2 ug)
O O
coumarin
O O
3,4-dihydrocoumarin
4-hydroxycoumarin
7-hydroxycoumarin
O O
OH
O OHO
esculetinO OHO
HO
scopoletinO OHO
H3CO
esculin
O
OH
OHHO
CH2OH
O OHO
O
O O
ONa CH2
OCH3C
warfarin
0-40%B(0-30min)
0-50%B(0-50min)
0-50%B(0-50min)
0-60%B(0-50min)
0-60%B(0-50min)
0-60%B(0-50min)
0-60%B(0-50min)
No.TI625E
TI625E-KC08
Polyethylene glycol (PEG)
Cadenza CW-C18, 250 x 4.6 mmA: water, B: acetonitrile1 mL/min (17-18 MPa), 37 deg.CELSD (spray chamber 50 deg.C, drift tube 100 deg.C)
polyethylene glycol #200025-30 %B (0-46 min)
2 uL (100 ug)
OCH2CH2 nOHH
0 20 40
0
100
mV
min10 30 50 60
150
50
polyethylene glycol #400027-32 %B (0-46 min)
10 uL (500ug)
polyethylene glycol #600033-38 %B (0-46 min)
2 uL (100 ug)
No.TI628E
TI628E-JJ15Imtakt Corp./JAPAN ([email protected])Imtakt USA ([email protected])North America Other Countries
Scherzo SM-C18, 150 x 4.6 mm0-60 %B (0-30min)1 mL/min (9MPa), 37 deg.C, 270 nm2 uL (0.2-1.2 ug)
Coumarins
A: water /acetic acid = 100 /0.1B: acetonitrile /acetic acid = 100 /0.1
A: water /acetic acid = 100 /0.5B: acetonitrile /acetic acid = 100 /0.5
A: water /acetic acid = 100 /1B: acetonitrile /acetic acid = 100 /1
1
2
3
4
5
6
7
8
O O
coumarin
O O
3,4-dihydrocoumarin
4-hydroxycoumarin
7-hydroxycoumarin
O O
OH
O OHO
esculetinO OHO
HO
scopoletinO OHO
H3CO
esculin
O
OH
OHHO
CH2OH
O OHO
O
O O
ONa CH2
OCH3C
warfarin
mA
U
0 10 20 30 min
0
50
100 1
23
4
56
7
8
12
3
4
5
67
8
1
2 3
4
5 6
7
8
No.TI649E
TI649E-KC25
Data provided by Dr. Eric VERETTE, SEDERE S.A.S., FranceImtakt Corp./JAPAN ([email protected])Imtakt USA ([email protected])
North America Other Countries
Scherzo SM-C18, 150 x 2 mmA: H2O + 0.3% HCOOH, B: acetonitrile + 1% HCOOH0%B (0-2 min), 0 - 20%B (2-10 min), 20%B (10-15 min)0.3 mL/min, 30°C, 2uLELSD (SEDERE, France), SEDEX 90LT, 30°C, 3.5Bar
Simultaneous HPLC/LT-ELSD Analysis of Organic and Phenolic Acids
Organic and Phenolic Acids (12 compounds)
0 5 10 15
mV
12
3
4 5 67 8
910
11
12
MinuteIm
purit
y fro
m L
actic
aci
d
ng (o.c.) ResponseRTMinutes
LOD(S/N=3)%RSD (n=8)RT
* Semi-volatile compound
12- Gentisic acid11- Syringic acid10- Chlorogenic acid
9- 4-HPAC8- Protocatechuic acid7- Lactic acid6- Gallic acid5- Citric acid4- Succinic acid3- Tartaric acid2- Malic acid1- Quinic acid
4-Hydroxyphenylacetic acid
14.9713.6213.1612.3210.219.817.604.113.272.372.081.60
0.060.070.070.090.140.180.310.250.200.110.090.09
1.93.02.21.72.42.41.91.32.22.71.92.1
6.22.34.03.43.755.9*8.025.39.020.97.74.5
No.TI650E
TI650E-KC24
Surfactants
1 2
Imtakt Corp./JAPAN ([email protected])Imtakt USA ([email protected])North America Other Countries
Cadenza CW-C18, 50 x 3 mmA: waterB: Methanol70-80 %B (0-6min)0.35 mL/min (4MPa), 37 deg.C, 260 nm2 uL (2% acetonitrile solution)
C8H17 OCH2CH2 nOHn = ~10
Triton X-100
C9H19 OCH2CH2 nOHn = ~12
Igepal CO-720
1
2
mA
U
0 5 10 min
0
100
No.TI659E
TI659E-KD13
Farnesene isomers
Imtakt Corp./JAPAN ([email protected])Imtakt USA ([email protected])North America Other Countries
250 x 4.6 mmwater / methanol = 20 / 801 mL/min (14-16MPa), 37 deg.C, 225 nm0.6 uL (5% mixture of farnesene, THF)
(E)-beta-farnesene
0 20 40 60 80 min
0
500
mV
(E)-beta-farnesene
(E)-beta-farnesene
No.TI665E
TI665E-KE21Imtakt Corp./JAPAN ([email protected])Imtakt USA ([email protected])North America Other Countries
Scherzo SM-C18, 50 x 3 mmA: phosphoric acid / NaOH (25mM, pH 7)B: 100mM phosphoric acid0%B (0-1min), 0-100%B (1-4min), 100%B (4-7min)1.0 mL/min150uL
[ICP-MS]ELAN DRC-e (PerkinElmer)m/z 52
LC-ICP-MS analysis of Cr(III) and Cr(VI)
Inte
nsity
(-)
Cr 6+
Cr 3+
100 µg L-1 100 µg L-1
10 µg L-1 10 µg L-1
1.0 µg L-1
0.0 1.0 2.0 3.0 4.0
100
200
300
Retention time (min)
1.0 µg L-1
