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ChIR Symposium 2017 Faro, 3-4 July
Matilde Mazzotti (a), Flavio Guidi(a) , Gianni Sulas(a) , Guido Emiliani (a)
(a) Micoperi Blue Growth srl, via Trieste 279 Ravenna
• Start up company born in 2014 • Develop new technologies to produce
sustainable algal biomass • Guarantee of traceability and high quality of
its products • Core business: research on microalgae
cultivations
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3. Micoperi Blue Growth :” A blue growth for a sustainable future”
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ChIR Symposium 2017
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CONTENTS
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1. MICROALGAE
2. FIELD OF APPLICATION
3. MARKET
4. CULTIVATION..AND CONTAMINATION
5. INNOVATIVE STRATEGY
6. MISSING..
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ChIR Symposium 2017
1. WHY MICROALGAE?
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NUCLEUS
LIPID DROPS
CHROMOPLAST
CHLOROPLAST
CELL WALL Pigments
Chlorophyll
Antioxidants
Carotenoids
Oil Omega 3
Biopolymers
β-Glucans
The biofactory
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ChIR Symposium 2017
• Prokaryotic and eukaryotic • Wide range habitats • With diverse metabolic potential • Different organic compounds • Half of the O2 • CO2 sequestration
0
20
40
60
80
100
120
0 20 40 60 80 100 120
Air equilibrium
External CO2 concentration (mM) R
ate o
f C
ass
imilat
ion
(%
max
)
+CCM
-CCM
CO2 concentrating mechanisms (CCM)
1. WHY MICROALGAE?
Higher photosynthetic efficiency
More rapid biomass accumulation
than in land plants
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ChIR Symposium 2017
Differences with land plants:
Greater ability to capture light because of Rubisco enzyme and to adapt their PSI and PSII
CCM facilitate CO2 uptake when there is low availability of it. Pumped CO2 near Rubisco: high speed and concentrations
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2. FIELDS OF APPLICATION
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ChIR Symposium 2017
200s species:
• > 100.000 species identified • 200 species used in worldwide
sectors • Only 5 -6 species are today
commercially exploited
• Attractive for commercial exploitation in a low carbon economy
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2. FIELDS OF APPLICATION
Only DIETARY SUPPLEMENTS
(without anykind of processing except
drying)
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ChIR Symposium 2017
• Exploited for centuries as food !
• Clorella e spirulina
• Full of bioactive compounds to become innovative source for new food !!
COMMERCIALISED!!
EARLY DEVELOPMENT!!
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2. FIELDS OF APPLICATION
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VITAMIN B12 / 500 gr Meat
5 gr. Spirulina = 1 Spoon
PROTEIN / 35 gr Beef
IRON / 300 gr Spinach
CALCIUM / 3 glass of milk
pOTASSIUM / 1 tomato
PHOSPHOROUS / 1 anchovy
ß CAROTENE/ 18 carotts
VITAMIN E/ 3 almonds
ChIR Symposium 2017
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2. FIELDS OF APPLICATION
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• Potential to ricover nutrients from waste and release feritlizer
• Induce systemic resisthance in plants
against pathogens (MBG’s PATENT for ISR)
ChIR Symposium 2017
The heightend awareness concerning environmental preservation and resource scarsity has produced the need for a more sustainable AGRICULTURAL production systems
• Several studies performed on pets, fishes and farm animals: animal health growth and nutriotional properties
• Source of protein, color, lipids….
COMMERCIALISED!!
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2. FIELDS OF APPLICATION
High valuable compounds for :
PHARMACEUTICAL, MEDICAL AND
COSMETICS
EXTRACTS
ChIR Symposium 2017
Pigments: phicoerythrin, astaxanthin, phycocyanin, carotenoids..
PUFA’s: ARA, EPA, DHA,
Sulfated polysacharid, Beta-glucan, proteins….
Many species, intentionally stressed by specific factors (strong light, nutrients depletion, salt..) or during specific phase of growth can produce interesting compounds
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2. FIELDS OF APPLICATION
Marker Anticancer, Antitumoral,
Antiviral, Antioxidant
ChIR Symposium 2017
Specific advantages over synthetic alternatives
Many EU producers are stepping in algae exctract!
C
O
S
M
E
T
I
C
anti-inflammation, anti-aging, hydrating, suncare
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P
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A
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M
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C
A
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COMMERCIALISED!!
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2. FIELDS OF APPLICATION
NH4+ : 97 µmol g-1 h-1
NO3-: 13 97 µmol g-1 h-1
Mazzotti et al. (in preparation)
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ChIR Symposium 2017
• Treatment of watewater pollution
• nutrients and heavy metal removal
• Research since 1950 several wastewater and species
• Assimilation and bioaccumulation
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2. FIELDS OF APPLICATION Third generation of BIOFUELS
SOLUTION: INTEGRATED SYSTEMS!!!!!!!
EXPENSIVE!!
High PUFA’s
contents!!!
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ChIR Symposium 2017
Attractive reasons for microalgae in biofuels: • Marginal lands • Wastewater • Continuous
harvesting • High exponential
growth • High lipid • CO2 from industries
gas
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3. T
HE
ALG
AE
MA
RK
ET
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0
1000
2000
3000
4000
5000
6000
7000
8000
9000
10000
ton
ne
s d
ry w
eig
ht
total production volume
1999
2004
2011
ChIR Symposium 2017
MICROALGAE BIOTECHNOLOGY INDUSTRIES HAS
GROWN FAST AND DIVERSIFIED:
• 75% IN HEALTH
FOOD MARKET AS DIETARY
SUPPLEMENT • 25% FEED AND
COSMETIC, FOOD ADDITIVES
• IT IS GROWING THE HIGH VALUE
COMPOUNDS MARKET
SIZE AND VALUE OF PRODUCTION
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3. T
HE
ALG
AE
MA
RK
ET
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0
1000
2000
3000
4000
5000
6000
7000
8000
9000
10000
ton
ne
s d
ry w
eig
ht
total production volume
1999
2004
2011
ChIR Symposium 2017
MICROALGAE BIOTECHNOLOGY INDUSTRIES HAS
GROWN FAST AND DIVERSIFIED:
• 75% IN HEALTH
FOOD MARKET AS DIETARY
SUPPLEMENT • 25% FEED AND
COSMETIC, FOOD ADDITIVES
• IT IS GROWING THE HIGH VALUE
COMPOUNDS MARKET
SIZE AND VALUE OF PRODUCTION
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4. CULTIVATION: SITES & SYSTEMS
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OPEN PONDS: OPEN PONDS – GREEN HOUSE: PHOTOBIOREACTORS:
PRODUCTIVITY PER SURFACE
COSTS
SPECIES VARIETY
QUALITY
ChIR Symposium 2017
Every species has its own peculiarities in terms of growth parameters, and for this reason, but not only, research and industry have develop several type of systems:
Open ponds present a great dependence
on weather conditions and high
risks of contamination, they
have low maintenance and
constructions costs
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4. CULTIVATION: LOW PRODUCTION and LOW QUALITY
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References: Vardaka et al. 2016
«It is the best well known food supplement
commercially called Spirulina»
7,7
61,5
15,4
7,7 7,7 Europe
Asia
US
Central America
Oceania
ChIR Symposium 2017
An example of the importance of knowing microalgae can be found in the CYANOBACTERIA Arthrospira platensis:
Low globally production of 5000 tonnes per year despite of the high market demand
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Spirulina OTU 42%
Other Cyanobacteri
a OTUs 58%
n = 31
6,4 %
6,4 % 3,2 %
19,4 %
References: Vardaka et al. 2016
ChIR Symposium 2017
No specific legislation for EU algae production First report on molecular diversity of commercially available «Spirulina» 18/31 : one to five additional cyanobacteria Due to environmental competition and contamination
While Spirulina is widely recognised with healthy aspects, there are really few studies on the quality and the risks associated with this algae.
4. CULTIVATION: LOW PRODUCTION and LOW QUALITY
Low quality of many Sprirulina based products due to several aspects:
Contamination
No research
No cleaning
No quality chain
No technology
Fungi Bacteria Patogens Other algae
(c) MBG 2017 - All right reserved References: Vardaka et al. 2016
ChIR Symposium 2017
4. CULTIVATION: LOW PRODUCTION and LOW QUALITY
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5. A SOLUTION FOR AN ALL YEAR-LONG HQ PRODUCTION Combination of several aspects: high-tech greenhouse, monitoring and research
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Innovative facilities and cultivation systems
Morphological and genetical analysis
Monitoring and chemical and physical
analysis
ChIR Symposium 2017
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5. A SOLUTION FOR AN ALL YEAR-LONG HQ PRODUCTION Combination of high-tech greenhouse, monitoring and research
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ChIR Symposium 2017
Analysis Frequency
visual check Every 3-4 days
growth Daily
pH - °C - lux Daily
Macro nutrients check up Every harvest
Nutritional values Every 1-2 weeks
High value compounds Every 2-3 weeks
Microbiological analysis Monthly
Patogens Every 2-3 months
Physico chemical analysis of water Every 6 months
Pesticides and heavy metals Every year
Toxins Every year
Performed by our Lab or external and certified Lab:
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Species specific systems of cultivations OPEN
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5. CULTIVATION: SOLUTIONS
CLOSED
• ALGAE SPECIES • FIELDS OF APPLICATION • VOLUME OF PRODUCTION
• MATERIALS • RELATIONSHIP • SIZES
For each system:
ChIR Symposium 2017
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5. ALGAE CONTRIBUTION FOR THE HUMAN AND ENVIRONMENTAL BENEFITS
Production of High Quality microalgae healty
properties
i.e. Spirulina as microalga is a natural ″eater ″ of air
CO2 (O.8 kg of CO2 per kg of dry biomass)
Less water consumption
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BEEF MEAT
50 L
WHEAT
6 L
SOY
3 L
SPIRULINA 1 L
WATER CONSUMPTION PER KG OF PROTEIN
ChIR Symposium 2017
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6. Missing…
NEW GREEN TECHNOLOGIES FOR INDUSTRIAL EXCTRACTION
• SAFE SOLVENTS • LOW ENERGY CONSUMPTION • RECYCLING METHODS • SCALABLE • COST SAVING • EFFICIENT
COMPOSITION OF LIPID EXCTRACTION FROM
P. tricornutum
TRADITIONAL NEW GREEN
fatty acids others
Data from a collaboration with University of Bologna – CIRI Lab Prof. Tagliavini
ChIR Symposium 2017
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5. Missing…
NEW GREEN TECHNOLOGIES FOR INDUSTRIAL EXCTRACTION
« world wholesale market for infant market US$ 10 billion per year»
Spolaore et al. 2006 DSM’s algae derived DHA is found in 99 % of all baby food in USA (Enzing et al. 2014)
ChIR Symposium 2017
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Thank you!
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ChIR Symposium 2017