Chromium ConnectAutomate your single cell workflow
Generate consistent results Reduce single cell data variability
Maximize lab productivity Go from cells to sequencing-ready libraries
Integrated and validated Cell partitioning, barcoding, and library prep
10xgenomics.com/instruments/chromium-connect
Thermal cycler
On-deck thermal cycler for all incubation steps
Performs reverse transcription, amplification, fragmentation, ligation, and sample index PCR
We just 10x'd your productivity
Chromium Automated ControllerCompact and designed for seamless hands-off automation
Performs single cell partitioning and barcoding
GantryMovable liquid-handling pipetting device with precision positioning
Automates hundreds of pipetting steps for the entire single cell workflow
Barcode scanner2D barcode reader to scan all reagents and consumables
Enables experiment tracking and prevents reagent mix-ups
Deck areaIncludes thermal cycler, magnetic plate, and 10x optimized reagent carrier
Single cell partitioning, barcoding, and library prep—together in one optimized instrumentChromium Connect integrates components customized for the 10x Genomics single cell workflow, automation-specific reagents and consumables, and an easy-to-use touchscreen computer. From intuitive self-guided setup to error-proof reagent loading, Chromium Connect is designed so even new users can start running single cell sequencing experiments quickly.
10x optimized reagent carrier
Custom designed carrier for error-proof reagent loading
Fits pre-aliquoted reagent strip tubes for up to 8 samples
Magnetic plate
Integrated magnetic separator to handle all cleanup steps
Uses magnetic beads for DNA purification and fragment size selection
Prepare consistent single cell gene expression libraries with our automated solution Chromium Connect automates your single cell sequencing workflow, letting you go from cells to sequencing-ready libraries with walk-away convenience. This instrument enables you to generate consistent and reproducible single cell gene expression results across experiments and across users. Chromium Connect can reduce errors from manual pipetting and shorten your hands-on time from more than 8 hours to less than an hour. Maximize your day and run your single cell experiments with confidence using an integrated and validated solution.
Automation-specific single cell reagents and consumables10x reagents for Chromium Connect come in strip tube format for easy loading into specially designed carriers. Each strip tube is color coded and barcoded to ensure optimal placement on the deck. Flexibility for 1–8 samples means you can get final libraries every day, without wasting reagents or waiting for large batches. The single-use chip and gasket are also specially designed for use with the Chromium Automated Controller.
Benefits with automation
Hands-on time
Pipetting steps
Interaction points
Walk-away time
80%
90%
70%
80%
Touchscreen computer & softwareBuilt-in touchscreen interface with internet connectivity
Guides user step-by-step through fast and simple experiment setup
• LIMS compatible for sample, reagent and experiment tracking
• Embedded error-handling features for intuitive troubleshooting
Included on deck
10xgenomics.com/instruments/chromium-connect
Thermal cycler
On-deck thermal cycler for all incubation steps
Performs reverse transcription, amplification, fragmentation, ligation, and sample index PCR
We just 10x'd your productivity
Chromium Automated ControllerCompact and designed for seamless hands-off automation
Performs single cell partitioning and barcoding
GantryMovable liquid-handling pipetting device with precision positioning
Automates hundreds of pipetting steps for the entire single cell workflow
Barcode scanner2D barcode reader to scan all reagents and consumables
Enables experiment tracking and prevents reagent mix-ups
Deck areaIncludes thermal cycler, magnetic plate, and 10x optimized reagent carrier
Single cell partitioning, barcoding, and library prep—together in one optimized instrumentChromium Connect integrates components customized for the 10x Genomics single cell workflow, automation-specific reagents and consumables, and an easy-to-use touchscreen computer. From intuitive self-guided setup to error-proof reagent loading, Chromium Connect is designed so even new users can start running single cell sequencing experiments quickly.
10x optimized reagent carrier
Custom designed carrier for error-proof reagent loading
Fits pre-aliquoted reagent strip tubes for up to 8 samples
Magnetic plate
Integrated magnetic separator to handle all cleanup steps
Uses magnetic beads for DNA purification and fragment size selection
Prepare consistent single cell gene expression libraries with our automated solution Chromium Connect automates your single cell sequencing workflow, letting you go from cells to sequencing-ready libraries with walk-away convenience. This instrument enables you to generate consistent and reproducible single cell gene expression results across experiments and across users. Chromium Connect can reduce errors from manual pipetting and shorten your hands-on time from more than 8 hours to less than an hour. Maximize your day and run your single cell experiments with confidence using an integrated and validated solution.
Automation-specific single cell reagents and consumables10x reagents for Chromium Connect come in strip tube format for easy loading into specially designed carriers. Each strip tube is color coded and barcoded to ensure optimal placement on the deck. Flexibility for 1–8 samples means you can get final libraries every day, without wasting reagents or waiting for large batches. The single-use chip and gasket are also specially designed for use with the Chromium Automated Controller.
Benefits with automation
Hands-on time
Pipetting steps
Interaction points
Walk-away time
80%
90%
70%
80%
Touchscreen computer & softwareBuilt-in touchscreen interface with internet connectivity
Guides user step-by-step through fast and simple experiment setup
• LIMS compatible for sample, reagent and experiment tracking
• Embedded error-handling features for intuitive troubleshooting
Included on deck
10xgenomics.com/instruments/chromium-connect
Chromium Connect EnablesWalk-Away ConvenienceChromium Connect automates the construction of single cell, sequencing-ready libraries from cell suspensions.By increasing your walk-away time to up to 8.5 hours and reducing the number of manual pipetting steps, you can regain your freedom to focus on other essential activities.
PHASE 1Sample and ReagentPreparation
PHASE 2Single Cell Partitioning, Barcoding, and ReverseTranscription
PHASE 3cDNA Amplificationand Cleanup
PHASE 4Library Construction,Cleanup, andSample Indexing
PHASE 5Final Library QCand Quantification
Automated ManualINSTRUMENTSREQUIRED:
GEMCheck
cDNAQC
INSTRUMENTSREQUIRED:
8.5 HourWalk-Away Time
PIPETTING STEPS
CONSUMABLES
OPTIONAL QC
AUTOMATED
MANUAL
Learn more at 10xgenomics.com© 2019 10X Genomics, Inc. FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.SAM000080 Rev A Chromium Connect Workflow
Step 3Quantify library
Step 4Set up qPCR
Step 5Run qPCR
Step 2Run ChromiumConnect
Step 1Prepare cellsand reagents
Step 8Quantify library
Step 4Amplifly cDNA;Clean up
Step 9Set up qPCR
Step 10Run qPCR
Step 7Index sample;Clean up
Step 6Ligate adaptors;Clean up
Step 5Fragment, End-repair,A-tailing; Clean up
Step 3Run GEM-RT;Clean up
Step 2Run ChromiumController
Step 1Prepare cellsand reagents
15 1015 10
15 5
0 8
10 3
1 2
20 6
15 6
10 5
10 5
15 5
30+ 8
Go from cell suspensions to sequencing-ready libraries with walk-away convenienceMake the most out of every workday with the benefits of automation. Manual methods to prepare single cell sequencing libraries require over 8 hours and hundreds of pipetting steps. Chromium Connect combines single cell partitioning, barcoding, and library preparation in a standardized, automated workflow.
This minimizes pipetting steps and decreases hands-on time to less than 1 hour. Forget about sample-to-sample and user-to-user variability, or even site-to-site handling differences. Let automation do the work and maximize your lab productivity, your walk-away time, and your resources.
© 2019 10X Genomics, Inc. FOR RESEARCH USE ONLY AND NOT FOR USE IN DIAGNOSTIC PROCEDURES. LIT000064 Rev A Chromium Connect Product Brochure
Learn more at 10xgenomics.com
Chromium Connect service and supportOur automation experts are dedicated to helping you get the most out of your 10x Genomics instrument by offering on-site installation, preventive maintenance, and remote support.
InstallationWith this service, our experts will install and verify the instrument for proper performance.
MaintenanceTrained field service engineers will conduct regular preventative maintenance tests.
SupportTechnical support staff are ready to troubleshoot and respond to any instrument issues.
HEPA CAP filtration optionFor labs that need to process samples in a clean, positive pressure environment, HEPA Clean Air Protection (CAP) filtration system is available for Chromium Connect. The integrated hood system saves space and monitors airflow, temperature, and humidity.
Chromium Automated Controller for partitioning
and barcoding
Magnetic platefor bead purification
Thermal cycler for amplification and incubation
Inside Chromium Connect
Sequencing-readylibraries
Data analysisData visualization
Single celllibrary constructionSample prep Sequencing
Enabling seamless lab operationsChromium Connect works as part of an end-to-end single cell sequencing solution—from sample preparation support to turnkey data analysis and visualization tools.