EVALUATION OF THE BACTERIAL INHIBITORY/CIDAL ACTIVITY OF THE CITREX® MOLECULE IN : 1) FOOD DIRECT CONTACT SURFACE AREAS 2) FOOD PRODUCTION ROOM ENVIRONMENT HAROLD EDUARDO DURANGO G. Medical Mycology Bacteriologist. Professor of Microbiology and Parasitology. Faculty of Medicine, U. of A. JOSE IGNACIO BARGUIL P. Food Microbiology Bacteriologist. Technical Consultant in Food Protection. Consultant for Banco Interamericano de Desarrollo, BID. Technical Director, Continua Consultores. Pediatric Infectology Research Laboratory, H.U.S.V.P.
Transcript
EVALUATION OF THE BACTERIAL INHIBITORY/CIDAL ACTIVITY OF THE CITREX® MOLECULE IN :
1) FOOD DIRECT CONTACT SURFACE AREAS
2) FOOD PRODUCTION ROOM ENVIRONMENT
HAROLD EDUARDO DURANGO G.Medical Mycology Bacteriologist.Professor of Microbiology and Parasitology.Faculty of Medicine, U. of A.
JOSE IGNACIO BARGUIL P.Food Microbiology Bacteriologist.Technical Consultant in Food Protection.Consultant for Banco Interamericano de Desarrollo, BID.Technical Director, Continua Consultores.
Pediatric Infectology Research Laboratory, H.U.S.V.P.
METHOD FOR SURFACE AREAS
• Surfaces, tools, and pieces of equipment in direct contact with foods for human consumption used in 3 different industries (i.e. dairy, meat, and agri-industry) were studied.
• All 3 stages of cleaning (i.e.: rinsing/removing coarse materials, detergent application with mechanic force [i.e.: scrubbing], and final rinsing/clarification) were practiced.
METHOD FOR SURFACE AREAS
• Two disinfecting concentrations (200 and 400 ppm) of Citrex® were applied by spray until dripping. The product was allowed to work for 15 or 30 minutes. Sampling was performed using PCA agar, for the determination of livable mesophyllic microflora, Violet Red Bile Agar (VRBA), and Brilla broth using the brush technique described by W. Mannheimer and T. Ibáñez (1971), covering 25 cm2 of the treated surface area. For total coliform and E. coli determination rubbing was performed on VRBA agar, while Brilla Broth was used for quantitative test purposes. Positive tubes were replicated then subjected to the E. coli confirmation indole test.
Cleaning, disinfection and sampling
Cleaning, disinfection, and sampling
CULTURE
Surface areas prior to and after treatment with Citrex ®.
SURFACEAREAS
MESOPHYLLICORGANISNSCFUs/25 cm2
TOTALCOLIFORMS E. coli
Small cheeseMolds
Countless Countless Positive
CurdlingVats
380 90 Positive
ProcessingTables
290 167 Positive
Lyre 276 68 Positive
Mill Countless 380 Positive
Cutter Countless Countless Positive
Inner pulpRemover
Countless 234 Positive
Small tools(knives, cutting
boards, etc.)
432 60 Positive
SURFACEAREAS
MESOPHYLLIC ORGANISMSCFUs/25 cm2
TOTALCOLIFORMS
E. coli
Small cheesemolds
43 0 Negative
Curdlingvats
12 0 Negative
Processingtables
8 0 Negative
Lyre 23 0 Negative
Mill 16 0 Negative
Cutter 7 0 Negative
Inner pulpRemover
67 0 Negative
Small tools(knives,
cutting boards, etc.)
2 0 Negative
Surface areas prior to and after treatment with 400 ppm Citrex ®
BEFORE 15 MINUTES 30 MINUTES
METHOD FOR ENVIRONMENT
• For production room environmental analysis prior to fogging 400 ppm Citrex®, OGY plates were exposed to the environment for 10 minutes. After Citrex® fogging new OGY plates were exposed to the environment for 10 minutes. All plates were incubated at ambient temperature for seven (7) days. Results were then read and interpreted.
Room environments prior to and after treatment with 400 ppm Citrex®
ENVIRONMENTNo. of molds and
yeasts, 10-min exposure
ProcessingRoom 1
12
ProcessingRoom 2
18
ProcessingRoom 3
10
Warehouse 22
ENVIRONMENTNo. of molds and yeasts, 10-min
exposure
Processing Room 1
3
Processing Room 2
7
ProcessingRoom 3
2
Warehouse 8
Room environments prior to and after treatment with 400 ppm Citrex®
