Lande Fu
Jun 6, 2018
Enter the world of crystallography
CO-OP in EMD Serono
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Before I start...
Try to find a question
I learnt the word crystallography for the first time when I was doing the interview.
Because I have no background on it, I don’t even have questions.
Just read the text book
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In the first few months...
Watch and Learn—wet lab experiment
Set up drops
Freeze crystals
Soak
Data collection
?
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I didn’t try to understand everything at once.
Instead I watch Ariele’s operation closely.
Look at other related experiments on the Internet.
In the first few months...
Watch and Learn—wet lab experiment
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At the same time...
Watch and learn—CCP4 refinemnt
Learn CCP4
Learn COOT
Understand the protein structure
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At the same time...
Watch and learn—CCP4 refinemnt
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Set up drops
Proteins grow in buffer
Soaking
Compound binds the proteins
Freeze cyrstals
Send to X-ray souce
Data colleciton
Get diffraction data
Refinement
ARI
Gryphon
Make the plate automatically Take images of each drops in
schedule.
Scoring the drops
Rock
Imager
First project…
Optimization, setup the automation
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Second project…
Antibodies screening—find the right conditions
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Extra project
Python script for PyMOL
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Extra project
Python script for PyMOL
Learn Python
Learn PymOL
Understand the Antibodies
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Extra project
Python script for PyMOL