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Collecting OrchidsIn the Venezuelan Orinoco-Amazon InterfluviumBy Gus tavo a . RomeRo -Gonzál ez and CaR los Gómez

Orchidacae are by far the largest

family of all flowering plants, with spe-cies occurring all over the planet except in its very coldest and driest habitats.

However, as is the case with most other liv-ing organisms, orchids are not evenly dis-tributed. Most species grow in the tropics; as we approach the poles, their numbers diminish rapidly. The United States and Canada, for example, have approximately 208 species; Mexico, some 1250 species in about half the combined surface area of the

two former countries, and Colombia has more than 4000 species in approximately half the area of Mexico. Ecuador is an even more striking example: there are more than 4000 orchid species in this country that has a little more than 25% of the surface area of Colombia.

Orchids are not uniformly distributed even within the neotropics. Their great-est diversity is found in humid, montane forests between 2600–8200 feet, and the orchid flora of lowland forests has long

been regarded as “depauparate.” Do these low counts reflect the actual orchid diversity or merely what collectors have detected? In other words, are there truly fewer orchids in these lowland forests or are they yet to be found? We will address this question using data from research we are presently conducting in southern Venezuela. We are seeking to document every orchid species found in the study area.

In contrast to Colombia and Ecuador, the checklist of Venezuelan orchids currently

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includes approximately 1500 species and it is not expected to exceed 1600 species. Why? Orchids are particularly diverse in montane forests found along the Andes, where some lineages of Orchidaceae have proliferated (especially the subtribe Pleu-rothallidinae). Colombia and Ecuador are privileged because several mountain chains of this complex cordillera are found within their borders. Venezuela, with barely a small portion of the northern Andes, shares only a fraction of this high orchid diversity. Within Venezuela, the number of orchid species is evenly distributed north and south of the Orinoco River, with the highest diversity found along Andes, the Cordillera de la Costa (and a few other isolated, adjacent mountains such as Sierra de San Luis) to the north, and the sides and summits of “tepuis” and “cerros” to the south.

Venezuela south of the Orinoco River, better known as the Venezuelan Guayana, includes three states: Amazonas, Bolívar, and Delta Amacuro, a region that occupies 175,000 square miles, slightly less than half the surface area of the entire country. About 88% of the Venezuelan Guayana drains towards the Orinoco, and the rest drains towards the Río Negro, the largest tributary of the Amazon River.

It is precisely in this small portion of “true” Venezuelan Amazonia (as opposed to Orinoquia) where our study site is located: Municipio Autónomo Maroa. This munici-pality occupies 5,405 square miles (about the size of Connecticut), has approximately 1,722 inhabitants, and is one of seven such political entities into which Amazo-nas State is divided. We have concentrated our collecting efforts in the different habi-tats found along the Yavita-Maroa road, a famous 20 mile portage that communicates the two largest rivers of South America. Yavita is a small village at the northern end of the road, which drains towards the Orinoco; Maroa, the capital of the Muni-cipio Autónomo Maroa, is at the southern end that drains towards the Amazon. The divortium aquarum, the imaginary line that divides the two basins, is found somewhere along the portage.

The trajectory of the road has changed dramatically since it was first reported in the literature in 1779 (by the Jesuit priest Antonio Caulin in his Historia Corográfica Natural y Evangélica de la Nueva Andalucia, on page 76 of the first edition printed in

Madrid) and since the famous naturalists Alexander von Humboldt and Aimé Bon-pland walked it in May 1800. The road was altered in the early 1800s, some years after Humboldt’s and Bonpland’s visit, and then again in 1978, but all three configurations generally cross the same habitats. Among these habitats are Amazonian savannas (savannas with few shrubs and true grasses but many species of Bromeliaceae, Cyper-aceae, Rapateaceae, and Xyridaceae, as well as a few Orchidaceae); “banas” or shrublands (basically savannas with a high density of small trees and shrubs, where a multitude of epiphytic and a few terrestrial orchids can be found); “caatinga” forest (medium-size forest with a fairly open canopy where, again, some epiphytic and terrestrial orchids can be found); and tall, tierra firme forest (where many epiphytic orchids can be observed, albeit almost always fairly inac-cessible in the upper canopy). Except for tierra firme forests that never flood and where soils tend to have some clay, all other habitats are subject to periodic floods and grow on white sand. Among the botanists who have walked and collected along this road were Alfred Wallace, Richard Spruce, John J. Wurdack, and Julian A. Steyermark (along the second road), and more recently, Otto Huber, Paul E. Berry, and Gerardo

Aymard (along the third road). In the past five years, our team has conducted collect-ing expeditions also along the northern border of the Municipio (Caño Mesaque and Caño Chimita in the drainage of the Atacavi River, a tributary of the Atabapo River that drains into the Orinoco), east of the road (Temi River, also a tributary of the Atabapo River, including one of its tributar-ies, Caño Tuameni or Tuamini), south of the road (San Miguel River that drains into the Guainía River), and along the eastern border (Casiquiare channel that, when join-ing the Guainía, forms the Río Negro).

All available references to the orchids of Venezuela were consulted when we initiated our project in 1989. After an exhaustive search, we found only eight orchid genera and eight species reported for the road and its immediate vicinity. By the time the Flora of the Venezuelan Guayana was published in 2003, reporting 157 genera and 732 spe-cies of Orchidaceae, we had already docu-mented, again, solely for the road and its immediate vicinity, some 40 genera and 120 species (the Flora of the Venezuelan Guayana is a monumental, nine-volume work writ-ten and edited by an international team of botanists and published by the Missouri Botanical Garden between 1995 and 2005). Currently we have registered for the entire

previous page: Acacallis fimbriata (rchb.f.) schltr. this page: Maxillaria superflua rchb.f. opposite page: (top left) Epistephium hernandii garay; (top middle) Catasetum pileatum rchb.f., female flowers; (bottom left) two views of an unidentified Vanilla species; (far right) close-up of Paphinia dunstervillei Dodson & g. a. romero.

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Municipio Autónomo Maroa more than 60 genera and 160 species, only a few of which we have not found along the road. This count includes several new reports for the country (e.g., Peristeria ephippium Rchb.f., described in 1883), one species that was previously known from only one collec-tion (Koellensteinia lilijae Foldats, first col-lected in 1958 and described in 1961), and at least six new species: Catasetum maröaense G. A. Romero & C. Gómez, C. yavitäense G. A. Romero & C. Gómez, Habenaria sp. Nov., Pleurothallis sandaliorum G. Romero & Carnevali, Sarcoglottis sp. nov., and Stelis bricenorum G. A. Romero & Luer. Flower specimens preserved in alcohol enabled us to accurately draw several species that previously had been incompletely, poorly, or not illustrated at all (e.g., Epidendrum apuahuense Mansf. and E. magnicallosum C. Schweinf.), as well as to correctly identify species or varieties that were misinterpreted in the past (e.g., what had been described in the past as Cleistes Rosea Lindl. var. pallida Carnevali & Ramírez is currently believed to be a new species).

Given the many botanists who had col-lected along the Yavita-Maroa road, how could such a large number of orchid spe-cies have gone unnoticed?

We hypothesize that the answer lies simply in time and space. Most plant col-lections are just points in time and space. Itinerant plant collectors, including the ones who have traveled from Yavita to Maroa,

spend only short periods of time (usually only days) collecting plants in a given habi-tat, usually not long enough to encompass the flowering time of all orchids found in such a habitat. Since it takes tremendous expertise to identify orchids without flow-ers, most collectors simply ignore plants that are not in flower. Sometimes indeed they do not detect all of them, since some are quite small and discernable only to the trained eye. Naturally, many species can be overlooked. Our strategy, although time consuming and costly, has been to sample every habitat several times every month of the year, thus maximizing the number of orchids we detect in flower.

A third obvious variable is expertise: knowing, a priori, what microhabitats are suitable for orchids and where to look for particular species has increased the number of orchids we have documented. Likewise, knowing the plants is also important: we can now identify more than 95% of the orchids we find even without flowers: the ones that we cannot identify are either new to the area or a very few that belong to particularly large and complex genera (e.g., Stelis Sw.).

Of course, the same case could be made for other habitats: the orchid diversity of montane forests no doubt would increase if we implemented the same sampling meth-ods we used along the Yavita-Maroa road. But that is not the point: we already knew that montane forests had lots of orchids. The question we wanted to address was whether

the low counts in the reported number of lowland orchid species reflected the actual orchid diversity or simply the collectors’ count. Now we have the answer: the low counts are a collecting artifact. There are many more orchids than we had anticipated. We simply have to find them.

Gustavo A. Romero-González is Keeper of the Orchid Herbarium of Oakes Ames, an integral component of the Harvard Uni-versity Herbaria (HUH) and the largest archive of orchid specimens and literature in the Americas. He is also editor of Har-vard Papers in Botany, the journal of the HUH. His research focuses on the Orchi-daceae of lowland northern South America and the biological basis for the long-term management of neotropical non-timber for-est products (including orchids, of course!). E-mail: romero@oeb.harvard.edu. Carlos Gómez, at the time of his retire-ment in August 2002, was supervisor of special services at the Dirección regional de salud, Departamento de Enfermería, Gobernación del Estado Amazonas, Puerto Ayacucho, Estado Amazonas, Venezuela. He is a medic with considerable experience, an avid naturalist, and an astute plant col-lector. He has a large collection of living orchids at his home in Puerto Ayacucho. He currently is writing an essay on the history of Chiquichique or piassaba harvesting, the fibers extracted from the leaf bases of the palm Leopoldinia piassaba Wallace.

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