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COMMISSION OF THE EUROPEAN COMMUNITIES !J

COM(78) 294 final.

£0M(78) 294 final.

Brussels, 28. June 1978. ccp ., - .. , B VL. c; :' l,j/

SERIALS UNJT

Report to Council and Parliament under

Article 2 of Council Decision (72/446/CEE)

of 28th December 1972 adopting a common

research programme in the field of Classical

and African Swine Fever

(presented by the Commission)

J ...

s;} 2 7 :,iftS Gl~T ~ :1".:c~.NGE

'"'t'·''¥ ... #';:• i'lllt'~~·-------~----------------

.. _;MI'liSSION OF THE EUROPEAN COMMUNITIES

• Directorate General for Agricultu~

, ~oordination of Agricultural Research

Report to Council and Parliament

under Article 2 of Council Decision N° (72/446/CEE)

of 28th December 1972 adopting a common research programme

in the field of Classical and African Swine Fever

INTRODUCTION

The first common programme of research on Classical (Hog Cholera) and

African Swine fever covered the period 1964-1971. Its basic objec·

tives were :

the physical and chemical properties of the virus, the influence

of physical and chemical factors on its stability, the physical

and chemical properties of the specific viral proteins, and the

structural and serological relations with other viruses for

purpoaes of classification;

the ~iological properties (1) of the virus with special ref!:rence

to cellular systems capable of ensuring viral growth~ method3 of

assaying infection~ se~ological methods of identification, viral

multiplication (1) of iutt."'r.feron and the ~elat:ion between the

properties of the virus and its virulence to the animal;

the setting up of differential diagnosis between Classical (Hog

Cholera) and African Swine fever from the different techniques

used for: the clinical diagnosis and the ma&::roscopic examination ' '

of the lesions, the histo·pathological analys:J.s and the viro ..

logical a.nd serological diagnosis.

----(1) in \?i.tro

The ~esults of this programme were published in October 1971

under the auspices of the General Directorate for Agriculture

under the title : "Properties of the virus of .Classical swine

fever and differential diagnosis of Classical and African Swine

fevers".

The general approach to the diagnosis of the Classical (Hog Cholera)

~wine fever included field examinations which could identify a

suspected outbreak pending laboratory confirmation.

For the African swine fever the approach .was the same. However,in

order to avoid confusioft at the diagnostic level; a distinction

was made between countries where both diseases existed and those

where only Classical (Hog Cholera) swine fever was present. In

both cases, but more particularly in the latter, laboratory

diagnosis was widely used to obtain ~ reliable confirmation of

the pr.esence or the absence ·of African Swine fever. In fact, at

the beginning of the programme, the typical lesions resulting for

the African swine fever disease were frequently observed; but,

subsequently, numerous atypical lesions akin, to the Classical

(Hog Cholera) swine fever appeared. Hence differential diagnosis

based on anatomical examinations, proved unreliable. During the

Seminar, organised·by the Commission in Lisbon in 1971, it was.

suggested that subsequent efforts should concentrate on improving

laboratory diagnosis.

The work initiated in the first programme was continued in the

second programme (1973-1976) which is the subject of this report,

and which was implemented following the Council approval in 1972

of the Commission proposed programme of resea~ch on two enzootic

viruses of the pig, the Classical and African swine fever viruses

(2). The conte~t of the programme was selected to.meet specific

..

•

•

•

I 11

objectives of the common agricultural policy and 1n particular to

minimize the economic losses caused by these diseases and to

promote the trade objective of the common agricultural policy (3).

RESEARCH ACTIVITIES

a) General

The task to carry out research on Swine Fever Viruses was given

to seventeen laboratories of different research institutes (Annex I),

of which fifteen dealt with Classical swine fever and five (in

Spain and Portugal and subseq~ently the United Kingdom France and

Denmark) with African swine fever. Several laboratories,

(in the United Kingdom, Denmark and Ireland) j~ined during the

second phase of the programme (4).

A new approach in t~e coordination of research activities seemed

necessary to overcome certain difficulties encountered in the '

first phase of the programme. To enable a tloser collaboration

betw~en the participating laboratories, the Commission invited

scientists of international standing to assist it by giving

~ advice on the scientifi~ content and_progr~ss of the programme.,

(2) Decision (72/446/CEE) of 28 December 1972, O,J. N° 298/50

(3) The main economi~ loes~s in the Community were been caused by the

Cl3asical swine fevar virus. The Afr.i~an swine fe~er virus is present in Spai~ and Portugal~ and represents a con$tant threat to the Co~nunity.

(4) Luxembourg does not have a national &gr.icultur~l research oervic~.

It works direc~l' in cooperatio~ ~itn other Membe~ States.

-4-

3eminars and Workshops were ozganised by laboratories in the

Hember States at the invitation of the Commission and after

discussion within the progra~e working groups• The host labo­

ratory made the necessary local arrangements, circulated papers.

and assisted the Commission in the choice of speakers.

Exchange of scientists between laboratories and visits of scien·

tists for trainin8 or information, were arranged on an ad-hoc

besis. Such exchanges and visits were essential to ensure full

• cooperation between laboratories and adequate access to information.

A ~~port was required from each scientist benefitting from these

grants.

The proceedings of seminars and workshops have been published in

' conformity-with the Commission's publication policy. Annex III

provides the list of publications resulting from the research

activities.

b) Main scientific objectives

The main scientific objectives of the programme were to isolate

the African Swine Fever Virus (ASFV) and Classical Swine Fever

'Virus (CSFV) and to isolate and characterize their protein and

nucleic acids. The availability of antigenic fractions of these

viruses was to lead to the development of diagnostic procedures

for quick and accurate detection of the virus.· The immune response

of the pig would be analysed and vaccines tested. In addition

the Commission gave another dimension to the programme, conceiving

it as a ~ool for promoting collaboration between scientists of

different countries of the Community, and for making sure that the

programme would result in practical achievements utilized directly

for the benefit of farmers.

'I

•

·~

l· t /

•

' ..

i'

-5-

c) _ Exchaqe of acientiats

Viaita to laboratoriea by the eo..iaaion's scientific adviser and

the exchange of techniques by .eans of exchanJe of personnel

shortened the tt.e required for standardization of techniques in

all the laboratories working ·on the suae subject, and for the

evaluation of results. li()rk on ASFV vas carried out in 1 security .

laboratories, and was restricted to the Iberian laboratories

(Madrid and Lisbon) and Pirbright laborat~ry. It was t.perative

that scientists fro. countries· t.aune to ASFV should work in

protected laboratories (5). ~ travel eXpenses of scientists were

paid by the Commission.

d) Exchange of materials

The programme.vas also successful in developing inter-laboratory . .

relationships, resulting in the transfer of virus samples from

one laboratory to others (vi~ the techniques necessary for

purification· and isolation of the virus as well as for its analysis).

Exchange of Virus strains and cell-linea between the different -

laboratories was essential to the development of the programme.

(5) To overcome the difficulties in developing the research on ASFV which was carried out in confined laboratories and therefore where exchange of materials was not possible and exchange of scientists was not easy, a scientific task group which included experts in iridovirus research, in electron microscopy, in bio­chemistry on molecular virology and in virology and cell biology was convened in tbe Madrid Institute working on ASFV.

·- -6-

e) Seminars and Workshops

f)

Discussions with scientists in d'ifferent laboratories indicated

considerable interest in·collaboration among scientists working

in the same field of research. Certain laboratories had developed

techniques which could be very useful as standard procedures. For

this r~ason, emphasis was given to organising workshops and

· seminars to enable dissemination of information.

All the workshops took place in 1975. Seminars were held on an

annual basis, one per year. They were organized in order to

bring together scientists of each participating laboratory, as

well as.scientists from third countries. The seminars included

oral presentations of research results and discussions of current

results and future needs.

The participants were divided into several discussion groups.

Their proposals and conclusions were brought before the plenary

meeting which adopted·final suggestions for presentation to the

Commission services and the Standing Committee on Agricultural

Research (CPRA) <6>· The list of seminars and workshops organised

is attached in.Annex II.

I

Harmonization of veterinary legislation

Closer co-operatio~ with the Commission's services,responsible

for agricultural legislation in veterinary matters soon appeared

necessary. Scientists, especially those involved in the epide­

miology of swine fever virus and the appearance and disappearance '

of epidemics, felt that the legislators could and should benefit

from the scientific knowledge accumulated in the field during

many years.

(6) Council Regulation N° (EEC) 1728/74 of 27 Jqne·1974, O.J. N° 182/1.

' ., '

•

..

.. . ~~----------------~------~~--~--~~

•

·-7-

As ~ result, veterinary legislation experts participated in the

seminars and workshops which dealt with epid'emiology and eradica­

tion of swine fever viruses.

In addition, a special Committee was established, including

several senior scientists and members of the veterinary legis-·

lation services, to discuss speicific policy measures for eradi­

cation of swine fever diseases. Also, a survey was carried out

on "Studies on the epidemiology an~ economics of swine fever

eradication in the EEC". This study was financed by the research

programme on swine fever viruses.

g) Finance

A total of 2.723,003 u.a. was allocated by the_Council to finance

the programme, of_which 2.450.503 u.a. for research activities

and 272.500 u.a .. for co-ordinated activities. The research acti-

_vities component corresponded to 50% of the total cost, the

other 50% being provided by the· participating institutes. The

·co-ordinated activities were financed by the Community in full.

At the end of the programme, approximately 80 % of the original

credit allocation wa9 spent. Th~ m1used differe~ce reflects

partly strict mao3.gem~nt practices by the Commission, and partly

the het that one of t.be major contractors 0 ~ INIA PORTUGAL) -

could not be implemented~ du.~ te;';l p·oli.tica.l eventa in Portugal. in

the spring of 1915.

. '

RESULTS

I. Classical swine fever viru~

The isolation of the virus and the analysis of its proteins and

)( Ribonucle;$ ~cid (RNA) were achieved. It is now known that one of

the viral proteins is the antigen identical to an antigen in

~ovine Virus Diarrhoea (BVD). The second protein of classical

fi'1.Jine f,ever is unique to this virus. In order to utilize this

k~o~ledge and put it to practical use further work is necessary.

The a:i.m is to use the unique protein of CSFV in a radioimmuno-

&ssav which will make it possible to detect specific antibodies . .

to that v·irus in pigs. With the available techniques BVD infec-

tion can be detected instead of classical swine fever infection.

BVD infection of swine does not cause any deleterious effect,

while the antibodies to this virus interfere with the detection

of classical swine fever virus infection. The development of a

radioimmunoassay to selectively d~tect antibodies to classical

swine fever virus will make possible the early diagnosis of virus

infection in young animals and breeding sows.

The programm provided useful information on the nature of vaccine

strains available as well as on the conditions for improved

vaccination with these vaccine virus strains.. In addition,

special attention was given to the immune system of the pig,

which differs significantly from' that of other animals. New

information on the epidemiology of this virus infection was

obtained. Finally, many new areas of research on the genetics of

this virus, the viral nucleic acid and proteins as well as on the

imm~e response in the young an~al were opened as a result of

the programme. .. '

,, I

•

,,

IX. African swine ·fever.virus

Though only few laboratories were engaged in research in this

field, signfficant developments have occurred during the last

four years.

The purification of ASFV was achieved and led to the analysis of

.the viral Desoxy ribonucleid acid (DNA) and structural proteins.

Initial information on the mode of virus replication was obtained.

Some of the viral antigens were isolated and partially identified.

Many fields of research were opened as a result of this activity

including a better understanding of the epidemiology of this

virus disease which threatens to ,spread from the Iberian peninsula.·

CONCLUSIONS

The general conclusion to be drawn is that this programme has provided·

some basic knowledge on the two swine fever viruses and that it has

opened the way to practical future achievements in-the fields of

diagnostic procedures.and in the development·of better field control

measures.

More specifically~ the programme has demonstrated that :

although reliable data is still sparse, a view seems to energe

that a cycle of low virulence virus infe~tion can be established

in breeding herds. The conditions in ·which this occurs cannot be

fully define~ untiY furthe~ evidence on the cb~racteristics of

herds infected i.n thi~ way is obtained from field test.ing a~tivi···

ties now in progress. It is suggested th&~ large self contained

herds produc~.ng their. 01,ru replacewm.ts an.d medium sized herdG in

ar~as with d~nse pig populatio~s~ where there axe regul&r ivtr~duc~ion8

-10-

of fresh breeding stock, and movements of piglets, are most at ~

risk of developing such a cycle. The situation in these herds

could be likened to a fire which is regularly receiving fresh

fuel to keep it burning. The exact mechanism of transmission in

these herds is not yet clear, but probably dependent on contact ·

between piglets carrying the virus, and newly entered gilts or.

young sows. I'

Detailed analysis of the data from affected herds and comparisons

with the characteristics of unaffected herds and areas is almost

certain to reveal the key factors that are responsible for the

development of the carrier herd cycle •. The prevalence of such

herd infections appears low (St) from work in the most frequently

affected districts of Lower Saxony, but the proportion of pigs

showing serological evidence of. infection within affected herds

is high (40t). Findings in simitar work in France have revealed

positive serological reactions .in 46% of a small number of sows

in suspect herds with reproductive problems. This evidence,

· added to observations on the small numbers of piglets in affected

litters that are capable of spreading the virus, as well as the

timing of infection in pregnancy necessary to produce this phenomenon,

suggest that the proportion of infective piglets produced in an

affected herd must be small.

Although reliable field evidence is even~re sparse and virological

evidence on the stability of swin~ fever virus strains ~s somewhat

contradictory, it does appear that virUs from carrier pigs can

produce the classical form of clinical swine fever. However, the

development of this overt disease in a herd aay take several

months, which suggests tha~ the.acquisition of no1'118l virulence

depends on successive passages io • number of susceptibl~ pigs in

the herd.

I

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i

For thc rarlorr outltncd lt rccrt bnlihaly thrt vaccl,natioa can

ov€rcorc tic carrlqf cyclc fa brClding hcrdr.. 'ft ebould

clebly reduce tbe rlsh of tble pbenonenon deseloping but,

tuartcly, lt gko rcduccr 'thc

pocstbilitie; of dctect'tng

prelencr of garrlcr lafectlon by eerological teltt..

lPItrc-

unfor-

tbca

' lt€ potcntial for spread. of gsl.nc fever by direct contact is hlgb-

because of -the rapid turaover in ipig herds, intene ity of produc-

ttoo syrtenr, denaity of populatlooo aad coqrlerity of merketing

ryrtene. [owever, lt her been shonn thet lf the cbain of contacts.

egn be follovcd gulckly ead thorougbly lt le porcible to contain

tbe infcction before large ilrobers of herds are involved. Iden-

tlficetlon of pigr aad reglrtratlon of novenente arc, therefore,

tbe flrtt piiortty ln q prilvmtion progrsmre Bo that when controtr;:'

beconce nccetsery quafaatLne aad other aeaeures can have maxinum

effect.

- fnfomatioq on the risk of' rpreail ,by lndirect contact, ig rather

contredlctory, The v{nre appeers quite resf.stcnt but it ie

generally aecepted thst tbe risk fros gutriving.vinrs is the

.environnent ls faf.rly eaa:i.Ly eliniaated by good hygiener' drsnfi-

gemeat end readily e.eceptahle official. controLs.

c The problem ef, Ewllt variea wi.th nat.ioaCItr, f,ood hebi.tn whj.ch

'deterrsine the poueibi.li,ties of, uncoeked pork gai.nf.ng entrs to pf.g

. feed" Sqperlence indi"eat*s thnt'tlre risks sf iat,rcducf.ng srsiue

f,ever froo heevily f-nfected eouatries is auclr that srech imparta*'t'{.oss sre lterd to junti.fy" Hsrwevs,ro this rixk aeln be nini.mieed

by ef fect,i.qre cou&rul" olretr fthe cookiug of owiltr t,o he fed te pigs "

Annex tr9 mrovi.des scheanauie f"nfc,rmntfqiru pe, 'Lhe sei,nmt,f,f,ie prol;Lemru

existing ,nt the begf.mi.ng of

dlnnes V pnow$.den i*nform.Eflf$t.i

tl?lq p{sgraftsne"

0r ths qiSfferpm"t, ruxtrgeel:s deatrt, 'rri*h

wi^thfn t"he pr+s,r."s&ra,* epd the s*prnI"ts aeihf.e,';'etfo

ANNE

X I

LIST

OF

PART

ICIP

ATI

NG

IN

STIT

UTE

S IN

TH

E CO

MMON

PRO

GRAM

ME

ON C

LASS

ICAL

AN

D AF

RICA

N SW

INE

FEVE

R

SUBJ

ECT

Cla

ssic

al S

win

e Fe

ver

Vir

us

cc.s

.F.v

.>

INST

ITU

TE

Bun

desf

orsc

hung

sans

talt

fu

r V

irus

kran

khei

ten

der

Tie

re -

TOBI

NGEN

(f

ed.

Rep

. of

Ger

man

y>

Inst

itu

t fu

r V

irol

ogie

der

Tie

rarz

tlic

hen

Hoc

hsch

ule

-H

anno

ver

(Fed

. R

ep.

of G

erm

any)

Inst

itu

t N

atio

nal

de

Rec

herc

hes

V6t

erin

aire

s -

UCCL

E f:B

etgi

Oin

)

Sta

te V

eter

inar

y In

stit

ute

fo

r V

irus

res

earc

h -

LINO

HOLM

-KAL

VEHA

VE

(Den

mar

k)

\

Lab

orat

oire

Cen

tral

de

Rec

herc

hes

Vet

erin

aire

s -

MAI

SONS

ALF

ORT

( Fr

ance

)

Lab

orat

oire

de

Pat

holo

gie

Por

cine

, S

tati

on

de

Rec

herc

hes

de

Vi r

olog

ie'

et

d'Im

mun

olog

ie -

THIV

ERVA

L-GR

IGNO

N (F

ranc

e)

TITL

E OF

TH

E PR

OJEC

T

-O

rgan

izat

ion·

of

c.s.

F.v

.

Org

aniz

atio

n o

f C

.S.F

.V.

-P

atho

gene

sis

of

sub

clin

ical

Hog

C

oler

a

-S

tudi

es o

n th

e p

rop

erti

es o

f th

e v

iru

s

Stu

dies

on

Vir

us v

acin

e.

-S

tudi

es o

n th

e "C

omm

on A

ntig

ens"

of

swin

e fe

ver

and

BVO

vir

use

s.

-CS

F va

ccin

e

Dia

gnos

is o

f C

.S.F

.V.

-P

atho

geni

s of

C.S

.F.V

.

-S

tudi

es o

n c.

s.F

.V.

Cla

ssic

al S

win

e fe

ver

vac

cine

.

-S

tudi

es o

n c.

s.F

.V.

Ant

ibod

ies

resp

onse

to

C.S

.F.V

.

~

Stud

y of

th

e im

mun

oglo

buli

ns.

Stud

y of

im

mun

ogen

icit

y. '

I .

SUBJ

ECT

i·

'

· IN

STIT

UTE

Vet

erin

ary-

Res

earc

h L

abor

ator

y_ A

BBOT

STOW

N­CA

STLE

NOCK

Co

Dub

lin (

Irel

and

)

Inst

itu

te Z

oopr

ofil

atti

co S

peri

men

tale

del

la

Lom

bard

ia e

del

l'E

mil

a-BR

ESCI

A (I

taly

)

Inst

itu

to Z

oopr

ofil

atti

co S

peri

men

tale

dell

' ~bruzzo,

TERA

MO

(It a

ly)

-

lnst

itu

to Z

oopr

ofil

atti

co S

peri

men

tale

dell

' U

mbr

ia e

del

le M

arch

e -

PERU

GIA

(Ita

ly)

Cen

traa

l D

ierg

enee

skun

dig

lnst

itu

t, A

fdel

ing

Vir

olog

ie -

LELY

STAD

(T

he

Net

herl

ands

>

Inst

itu

te o

f V

irol

ogy,

Vet

erin

ary

Fac

ulty

-UT

RECH

T· (

The

Net

herl

ands

)

Vir

olog

y D

epar

tmen

t, C

entr

al V

eter

inar

y L

abo

rato

ry-

WEY

BRID

GE

(U.K

.)

•

l •

TITL

E OF

THE

PRO

JECT

-se

rolo

gy o

f B

ovin

e v

iru

s di

arrh

oea

-S

tudi

es o

n c.

s.F.

v.

Vir

olog

y pa

thob

iolo

gy a

nd d

iagn

osis

of

c.s

.F.v

.

-T

echn

ique

s fo

r de

tect

ing

anti

bodi

es

Epi

zoot

iolo

gy o

f sw

ine

feve

r

-Im

mun

olog

y

Stu

dies

on

c.s.

F.V

. an

d(re

late

d ~iruses

-R

evie

ws

on T

ogav

irus

es.

Ult

rast

ruct

ure

of

swin

e fe

ver

vir

us

Ser

olog

ical

rel

atio

nsh

ip b

etw

een

swin

e fe

ver

vir

us

and

othe

r ag

ents

.

Inci

denc

e of

ant

ibod

y to

sw

ine

feve

r v

iru

s in

the

U.K

.

Vir

al c

onta

min

atio

n o

f fo

etal

bov

ine

seru

m.

.. :

·:.

I

SUBJ

ECT

'Afr

ican

Sw

ine

Feve

r V

irus

i I .I

-3 ~I

INST

ITU

TE

The

anim

al

Vir

us

rese

arch

In

stit

ute

-PI

RBRI

GH

T <U

.K.)

Dep

artm

ent

of A

nim

al

Hus

band

ry,

Uni

ve!s

ity

of B

rist

ol

(U.K

.)

Cen

tra

Reg

iona

l de

In

vest

ig~c

ion

y D

esar

ollo

A

grar

io,

Dep

artm

ento

Vir

olog

ia A

nim

al -

MAD

RID

(Spa

in)

The

anim

al

Vir

us r

esea

rch

Inst

itu

te

PIRB

RIG

HT

( U

.K.)

Lab

orat

orio

Nac

iona

l de

In

vest

iga~

ao

Vete~inaria -

LISB

OA

<P

ortu

gal)

Lab

orat

oire

Cen

tral

de

Rec

herc

hes

Vet

erin

aire

s -

MAI

SONS

AL

FORT

(F

ranc

e)

'r

Sta

te V

eter

inar

y In

stit

ute

fo

r V

irus

R

esea

rch

-LI

NDHO

LM-K

ALVE

HAVE

(D

enm

ark)

Cen

tro

Reg

iona

l de

In

vest

igac

ion

y D

esar

ollo

A

grar

io,

Dep

arta

men

to

Vir

olog

ia A

nim

al

MAD

RID

(Spa

in)

I t • ( . ! I

TITL

E OF

THE

PRO

JECT

Stu

dies

on

the

com

posi

tion

of

c.s.

F.V

.

-Im

mun

e sy

stem

of ~he pi

g

-S

tudi

es o

n th

e co

mpo

siti

on o

f A

.S.F

.V.

-C

hang

es

in t

he

bas

ic b

iolo

gic

al

pro

per

ties

of

the

Afr

ican

sw

ine

feve

r fi

eld

&{

vir

us.

Stu

dies

on

ASFV

-A

ssay

of

ASF

V.

-N

atur

e o

f re

sist

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irus

-

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•

ANNEX ll

List of Jemin~.rs and Workshops organized for the Pu:r;l'Cse or · co-ordination_ in the Classical and African Swine fe.ver · prcg,.amme •

• J. Seminar on "Porcine Immunology"

'l'hiverval-Grignon, September 17-18, 1913.

2. Seminar on "Studies on Virus Replication",

Brussels, Mq 2 - 4, 1974.

l '

3. Se~tinar on "Diagnosis and Epizootiology ·ot Classical Swine f'eve1-",

Amsterdam, April 30 - Kq 2, 1975.

4. FAO/EEC Expert Consultation on the

swine revers,

Bl"a41oatioaot Atric&D and -Olaasical.'

Ha.nnover, September & -10,· 197&

II. WCRJC3HOPS

1. Workshop on WVirological Methods",

Utn•cht, September 2 - 6, 1974.

•·'

2. Workshop on "Immunologic~ 'l'eChni«Ne"". I.

Lindholm, June 9 - 14., 1975. •

. I'

3. Workshop ·on "Diagnos~s ot African Swine Peyer",

Madrid, February 24 - 28, 1975. :

. . . ' 4. Workohop on . "Di agt'lOsi s ot · Clusical -Swi~ Fever",

Lelystad, Ra.y l,: 197,. ·

I

' .. 1 • •

5· Work&hop on "Concen~ration and purifi-cation ot· Hos .Chol.exoa V~!',, .. ~a, March.'~ --~4., l975• . . . .

..... -

'.'

\

6 •. ; Workshop on-•Piu-i·t~cauori and ConcentratioD.ot Uric~ Mne t~ver vi~~o". -._

.Ma4ri.4, Sep~'ber ~8.- 2&; 1~?5 .. : .

- •• ti I T. 1. ... -· PL $f Jw"' a &I I' uc•:tQ tatlk.,._.

'.-t. lt lt-, | _..;lr..r,,Li..ji :' '-i ,*r . *;a. ,

ANNEX III i ;''

---.+-'. ttst oF PUDtxcATIouS nEsutTllrc FRot{ lllE'ngslRcH'

0$ srtnm FHyER vrRUSEl

I. E'firDtSrCRFCllU$CS.1$5'f]tI/r r"Jn UrnUSm.{llKIrqITg$. XER TIERE - lputsntqBlire:i

FAC/EEC eonsdtation on Eradication pf, Ctassical arri Afrieen slrlno fevergE$i:ilCYflff , 6 - IO September 19?6.

t,l' ..

' rt'x,, ,

t.'+

'+.lt'

| '''I

t

Tl""i"s 1ist of ref€r€nces does not lncludt the' papersl DreEented in the

-F"o*if'G' ffi:: ;::ffiffi;""Iff:::T:ffIr:il:.h"deE Vinw naob sttrmulierung der "I{rmbbo?.Jrtealf.- Berl. llttnch' lflor[pztl, Wschr. 9€, 44749 (fgff)

,,

- K0tlsr 0., xAlflm,fiElxj, W.r [OnEtIz, ih. artd JAlSJtsIKr J, ' '*

llben elr$ 0lobuttn (fan"perrln) . urd Antfk$ryerbildrurgs-

atUrung wsrrend dps lcrrkopeniesta,rtltrns .der Scbl.pi.ne- ,

. p€sterkrankung,

Z, Inrnun.-Forecb; .3d, !1ll, gflg-f5! (tgff)

- KIIH, O.r und IOREilZ, R.J. l

, i Slutbildrrntereuchwrgen bel verschiedenen Verlaufsfomilnder Schrninepestt nfektlon unf,er besonderer Bcrtlckeichtl-gutl8 von lgmphoaytUncn Realc t,i onsn Tollechatten, lnrre6ur{frr-forrnen von Q6phoayten (olye,raytentr ) .foa lfuochen oark-res.ktionen

- llATIEAEllS, tf"

- I{ATTEAEIF!' tl. trrrd trORII, C.

(tgta ):r,ll

rlI

h

Da^s Terbalten .von prEzlpitlerenden Snbstanren aqs norealcn0r6arran'rnrd Zetlsn ln Vergietch zu defr iler prgrtgltierendenPanlcroassiutt€Bng Elt Sch.relnepest inf,igidrto,. S"h*irro-.t!bl. Tot,. ffia{li ,1, .13, a}g-e53 ttp$)

t ri

..t t.l

, - TbL. Dalct. &gi, I, Abt. Otsig. A.&' Ze-lzl (tlf4)

wrd K0ttR, 0. ' r;

tssr die Auftrennturg voneuropafsche Schrnt nepestnu:g des InnrarosLerenden

- .lrchiv ftlr dls geaarute

I

a

,,,1

Kri stallviolettvakainen EBgen rtiezur fgolierrrng urd, Chardsterlsie-Agene. ,

vfrus forschungr S4r 133-14.1

: '~ '.

\ I,· • ·~, , ~ .t ; ' . '

' • • ."" • ' l ,~ > • ' ..;

,' .. ~ . ~~

'• ' '•'

. :.· -'· .,, -

',· '," .

' . :U.TTHI.EU3, w •. utld KOiiU; ·;C_. "· ,

. . · ~rolo«i:sehe Id~ntifizierunz und VorhalttJrt de: hoch-.. . virulent~ 3chweinepeatvi.r~ in der .Immu!10elect_rophor.!t-S,e

,' ·· -;. Zb1.·, V•~·. Ked. B, a!• 47-59 (1975) . -- :_ · . . ' \ '

' . ' . ' '' mmr. o. um !amiAW~, ~~-- . ...

· . · · · .· Kr~hei ts-1 tmmunvorgtln&e und Virulentve·r~dCJl'U."\;,""en ·~i ,: ·

' ; _ der · Seh•i~peat ala -Reaktionon d~a betrotl'e~n· tvmph.:>~ ·

·. ,JVeloid.en S:fatems l . , .. ' ' '

· - :Berl.''Jrunoh.-Tierlrztl. Wsobr-. 88, 6 -. 9 (1975) .. ' ' ' -

:.tl'l'THAEU3,. W., JCCRN, G. und SCHt.o'l'TER£R, E. '•

XORJ, O.

ImnianoelectJ:,"opho·retJ.sohc Sttliien tiber Character imd~ :pe~ifi -.-.

-tit der Immunprlzipitate von Sohweineseren und ·Zel~?en•

sionen nach der. Infektion mi t hochvirulentem Virus der .

europarschen Schweinepest Zbt. Ved. Med. B 22,· in press (1976)

Isolation and cha;acterisation of the immunizing neent or ·cr.;·a,al violet vaccinee 8£8in~t HOg Cholera

. . .

Aft enhancin« effect on the process of swiM rover diaeanc

·~'the Virulenee b.Y stimulating lymphoeytea

c.z.c •. Sembw• 9n •Porcine Immunology". ,Thiverval...Cri·enon · · · t7,~~18th September 1973 (El.JR 5450) . . . .

...

,., . '

~· .

.,

"ORll, a. and MArr~us, \i. , Ou the :1Jelec.rti')n· ot pig ifm.una

' I'A- , sera tor c~~atio~-in

' ' ' aWino· tever diagq.putic . '

C,E .. C .. ~minu- OD "Dia.enosi.t3 &.nfl E~izoot.iolog-,r or Cla~aionl .:: . i , •

Stdne Pevereo .Amut~rd.am? ~Qth Ap:ril·-2nd M.a3 1975 (Etm 5486) ,I

., ' .. . . . . '

I ·•

I' ~ I \

- 3 ..

:u .. ITW'!'!'T'U'l' FfJR VTROII'CTF. 1F.R TIERi~P.7.TLTC?.BlT Hor.:T!1CP.ULE - P.A!ir.:)".'rn --trr..:JS, B., B.R. FnEY, U. BUC!<3CH, ,B~ ROEDBa ::.nd J. FR031'

Hog Cholera viral antigens (brief review) and Qome recent

re:Jults on the relationship to envelop antigona'or Bovine

Viral Diarrhea-K~osal Disease Viriona.

C.E.C. Seminar on "Studies on Virus Replication" Brusuelo,

2nd - 4t~ ~ 1974· (EUR 545I) .-.

''.

LIES!:. B., B.R .. FREY, D. PRAGER,• s .. M. HAFEZ and B. ROEDER ,• ..

The couroe or the natural swine tever virus infection in

individual swine and investigations on the developmem.

ot inapp-.,rent SF irafectiona.

C.E.C. Seminar on ·~Dlagnoa'is and Epizootiology or Clwa:Jaical

Swine Fever" Amsterdam, 30th April - 2nd M"' 1975 (EUR 5486) · I

LIESS, B. and D. ~OER . ,

)I)ElmiO, V.

Detection or neutralizing antibodies (NIF teqt) : ·~

Use or new technical equipment (CCSC System) for laborator.r ·· ·

swine fever di&&nosis.

C.E.C. Seminar on "Diagnosis and Epizootioloa of Classical 0 l .,

Swine Fever" Amate~dana, 30th April - 2nd May 1975 (EUR 5486) .• ,) . .

I ' :

The purification or viral glyooproteins by atfini +- cl.U'o:na- " I • V

tograp~ on Con A Sepharose r, .1

C.E.C. Laboratory J!tanual tor research on Classical and

.Urioan Swine Fever, EUR 5487, ;52-55 (1975)

-. '

III. I!.~TI'l'U'l' DA'riOWAL 00: RECRERCHES VETERilUL"CtES - UCCL'!

CHARLIER, a~, LEtJNEN, J., WELLErUNS, a., STRCBBE, R.

I.EUIEI', J.

De electrotoretiac~e mobiliteit van VarkeDSpeatvirua ~n RunderdiarheeYir\18 ,,

V&cciraation contre ;la Peate poreiJle et poneur de~~~ jam. Ked. 'Vet. !!2~ 93-103 (1975) · ·

'. '

I 1'1' .. ,·.

; . ,,.·H t .; '

t:' .··. ·=': . .. . . . ' ..

. "t.. ,_, ;.~

t~f~ ' . , .. :' ... ...... 1 .. ;1'

. "

'•.'

.... '·

CBARLIER, C. , SIJ'ROBBE, R. , ,.

.. '

1!0 AEM'' "A.. . . . -

V AN AERT, A..

VB AER'l', J.

1 ~.. varkensoera . · .. , 'i

Vlaams ~erg. T1jdsc~. !l• 24~-252 (1974)

The immunolog1c8J.ly related swine fever and mucosal

disease precipitino~ns and nucleotide metaboliqm

: Vlaama ~erg. 'l'ijdscb. !14.• 349-364 (1975)

Purification and some properties ot swine ~ever related

mucosal di•ease precipitinogen

Vlaams Dierg. Tijdscb. M• 339-348 (1975)

Purification ot the ~vine Viral diarrhoe~Hog Cholera

antieen

C.E.C. Seminal' on "Porcine Immunolog" Thiverval. Crignon,

. 17th-18th September 1973 (EUR 545p) " .

-~

LEUHEJJ' J. ' 'PLORE!rr • A. Di&&nosia ,and epiEootiology ot Classical Swine Fever in

:Belgium

C.E.C. Seminar on "Diagnosis and epU:ootiology ot Clanolcal

swine tever" Am~terdam, 30th ·April - 2nd Mey 1,75 (EUR. 5486)

IV. STATE VETERINARY INSTITUTE FOR VIRUS RESEARC!J- LINDHOJ.M • •

ASU, J., BA.$SE, A., OVERBY, E. 1 ,,

Pathogenici t7 t()~ the· pig Bet\l8 of two German. Strains

ot Hog Choler&

. !ford. Vet. Med. to be published (1917),

lllLSCAARD,. JC. and OVERBY, E. . '

Vaccination ot pies ~ainst Hog Cholera (Cl.assical swine Fever) w1 th a d~tere;ent split vaccine . ·~

,, I -

Acta Vet., Seand.', ll in plan (1976)

ESKILDSlm, K. and OVEiUJY, E'& . Serological dia.gnoGis or Cla.s~ical swine f'e.ver a compari- }

aon ot a moditied 41reot complement fixation te•t with an

.. '· ••

~-

:•

·,·

'irnmunofluorcacenc~ ·pl~e ncutralizatio~ toot in the

dia~sis of experimental subclinical infection.

Act~ Vet. Soand. !2• 131:~ 141 (197~) ·

Immunization again~t Swine fever with a Danish bovine

Viral DiPn"hoea Vil'l~ St:ra.in UG59 liox-d. Vet. J.!ed. ~. 497 - 503..-(1973)

()'!l"ER.BY, E. and SCHJERNillG-THIESEN:t K. lQ.['.ssik svinepest- et aktvel t problem for f'aellesmarkeds

londene

Saertryk Med. DaMke llrriaeg, 2.2, 553-555 (1973)

O'VERBY t' E., and SCHJERNmG-THIESEl~, K. "'

Afriknnak avinepest (ASP) ogdeM udbredeloc 6 Europa

S~rtryk Med. Danske ~rlaeg, .21• 411 - 414 (1974)

Subklinisk Kl.aasiok avinepeot. Etaktuel t diB£nosti::~jt og

immunoloeiak prob~em v~t"' 1

Saertryk Da.nokA $• 917-923 (1975)

DALSGflAR», K. The tcchr,dque of' quantit,ative eleetrophoresiu (crooocd

immunoeleetrophoresia) and ita application in the stu~

ot viral antigens

C.E.C. Seminar en ~studies on virus Replication" Eruoseln,

2nd- 4th MS¥ 1974 (EUR 5451)

SCHJERIClro-THIESEN, lC.

Di~'7lloatie procedures in Denmark i~ case cf susl'ected

classical swine 'ti!ver ,j :--.

r . •

...

t ·'

...

C.E.C. Seminar o~: "Diagncaia and Epizootioloa of ClClasica.l

swine fever" Ams'terdam, ·lOth April ~ 2nd J.~a;r 1975 (~ ... 'R 548G.

,, f

DALSCAARD, K. Co-~uthor in : "Studies on Atri~an SwiJ;te fever vi-rus z puriti.eation ::.r.d

analysis. or Viriona" to be pu'bliohcd·, 1977 {!=:JR 552-G)

ATNAUD, J.X .. , I.ARENAUDIE, B.

·.· ' •· .. . ~ .,

. :

... " ·~

, .. I

, ..

', ... ,,

I • . :..•.'o:r-·~ V;~-! ..

• • : .. 1, ·:-;;.--,

~-" -· .... . - . ... r ,

'I ' .. ,·.' , I '• ' ~ :' l .. • • • •

I "1'·' , • •,' ' ' •·:• ~;:~~-- • "t :\ •: ~ .' ' .' . ·' :· ~ · ~·, · ,· .. ?· _,.Demonstration ot low vir'dlent strains 1n tissue ·.•. :. ·.J . • ,

' ':.landktection or neutralizing antibodies .

culture

. .

O.E.C. Seminar .on "Diagnosis and Epi?.ootiology o!' Cla;;sl cal ;~.

swine rever", AmsterdaQ 30.th April - 2nd J"ey 1975 Cr:rm 5481:)): .. · • I • ~

VI. LA'RyRATOIRE Y!E PATHCLOGif*,PCRC!NE - ~TATION ll? RECRERCHES DE. v;rRcLOC!~ E'l'

D•r..·~:'mO!.OOIE - THtVZRVAL-C!UCl:ON.

METZOER, J.J. et PERT, P.

.. ' r\ ~. '" ~·. ' . ..

, I • ~ Structu; .. al features ot the. porcine immunof:lobultn G. inter-\:.

chain dieulfide·bridgea. ·B.ll.R.C., ~. 1,253-259, 1973. _. '·

Lea imrnu..-,oglobulines M poreinea. Analyse des ~~~es pc:,.ty-, .

peptidittues consti tuti ves de la mol,cule~

Ann. Re eh. V4t~r., !• 599-611, l97l

Taleuoe et arn.Jai ~~ aea ~\! .:orps ':#- Ulti-!:.~u~~a ..

C.R. Acad. Scl, !I§ (D), ll~5-339S', l9'f4.

ME'!'ZOER, J_.J. et !OURDIEU, C.

Radiolabelling ot porcine Ammunoglobulina

l~me I.P.~.s. ~on, !le 1, juillet 1974.

JIC'lD-'l'D, X., IOU7.E, P., ~~ ;.z. . t Chllr&~eriaation ot the humoral iiMiune response in thfl

piglet.

3~me I.P.v.s. ~on, li• 1, j~ll~t 1975.

RCUZE, P., HOUDAYER, r~., et KETZCER, J.J.

. ..

..

,·,

Immunological re:::Jpons~ in' piglets : Ettect or paaoiV·i~ Gpeci; tic antibodies. · .,

3eme I.P.V.s. ~on, li• 1, juillet 1974~

ME'!'ZCER , J. J .. , ROUZE , P. , BOURDIEU v C.. et HOtJDAYER , r.f. ,,

.. ,

Lea anticorpa homo~Jtotropiquee chez le Porce Journ~ee de la Recherche porcine. en Franoe. pp. 385-390, 1975.

\ .. ,. '-I!•

.. . .. . . . . .(;.. •,

i.-'1 . ...-f· ~~.,: 7-.. ,, ... _ .... I ~ . ' ,. <:·· ..

BOtQ, P.; BOUDA.YER, M. et JIE'l'ZGER., J~J • ., La r'ponse immuni ta.ire du Porcelet :, Ett'et de l'imm~ te

passive qur l'_i1DIIuniaation du porcelet indui te l 1 'aide

cl'un anti~ne inerte._ le lysozyme.

Journ4~a de la Recherche 'po .. me en Prance, ~P· 363-370,

• 1975·

~aD, J.J.,· JOURDIEU, O., ROUZE, p,. et H>'ODUER, X.

Heat aenai ti vi v ot JtOroiM l&O• J ._ Imalunol. Xeth. 19'f5 •

! (3), 295-300· '

DrZCim, J.J. •t PERT, P •

.. Lea Igl et 1 'i~t4 locate. . •

Reo. Keel. Vet~~; Altort, 151 (12), 761-767, 197.5.

Lea reaotiODB d.11{ypersensibilit4 chez le Pore. . . Reo. Med.. V't4r.'l Jlton, !8f3, 169-173, 197~

arzaEB, J.J. et PERT, P.

Lea r4aotiona iQ~~~uni tairea chez le Veau.

En 1 'ouvrage de .tJ. MORD.l' •La Pathologie clu Veau" (en

preparation). r.

La protection chez le Porcelet nouveau-n&. Vet4rbaai~

fr&.n9aia supplement au n• 1, PP• Jl-36, 1976.

!'I'

" .

·, .

Partial i'Dhibi -£ion ot the hlDiloral. iliUI\lD.e response ot piglets ii 'I . ·

against a protein antigen atter earl¥ postnatal immuni za- _

. tion.

Ann. Rech. V4t.' l971 (in press). . : .. i' i

AtnUD, .T,I,, OOR'l'tm:R, a., LAur£, H.

IDtluence cle l'ifDIIluni t4 paaaive tranaaiae par le coloc.trum I .., , ,

sur 1 ''vol uti011 de la Peste PorciM obez le porc .. let •.

Ann. Rech. Vet.',· !• 359, 1973.

. . . . OORTBID, O., ADAUD, J .M. (' j\

Xaladie ·des llwJaeuaea : mise 8D tridence de \Pari-.tio~ ami-. pntiquaa ent~ ti verses aoudlea A. 1 ''ai4e 4e ~- technique

.... . de a&roneQ"traliaiLtiqn .en cul tw-e oelluein •

ADD... Beoh. V4t1 , !• ·7?• 1973 • ' ' 1 4

lrf,Am, J.r,'G0a$EIERT C" r

I

mnrEIEBo O"

\

l.llfiE, I,i.

,t

IJm, f,n .

Lfin8r E

s$rffirl fi*

vtrus en Francd ct'lcUr r01e cf,''rrtp t !€volntlon d,a la nalsr{lc tous se fofuo gubral{ntqtlo on chnonlqut olp }G te$rain.

t:a r'

Itrrr. Rsol. Yst. r Ir Tl t l9?4ti

:rl

C0B[EF|f,? .0. r .lX'ItUDl J.f,' r CILICERT C. n ,@LF!!r t.:.', lotlvttd antlgsnt{rlr ootrDirrde Cr dsu- togartrus I lc Vtms't --

i '

-rds la Pcgtc porotpe ofar{}qw ct le virrus its 1* pnlndl,l

ilrs nrquo[aair :':

Rcob. Vct., 5, 3?3, l9?4,'

Seg Cholur rrlnlr E ooryai'lson of tuo lll vl3ltf; aautr*ll-Glng aatlbodlct tttration netboda,

ADn Bscb. Vot, (eoua preasei Lg76.: r. rt

I

Inprory€i rsthod. for tlu gurtftcstton of Fog abol,crr vlrun.'lrchf o of, Vlrolocgr (raocptca) t976.

,:.:

Scnsltl?ft of,.-$6 o[olcra vlrns to bitrplftio cnn0ns+,,.ld;$tcb.Tct.(mur9rcaac)L976.:...':' |'

, - t, ,:.j " ;

" ' ' .- .' ',.j,.: t ' i

1

I

.floolrctrtofo*rfog og Eof cholsra ylnrt. - ' '

,IlE;, Bssb. Tctr (roua prceee) LgT6.

&rer lfisd. Yot, n ffio 54?, Ig?da ,

, I,

't I

Strlus fnmp r *n*lusnse of 'p*emi.u* $.$ffird$

:i :'

nroaiD, o, r '[smIRWi &, cuJcER?ct IAI'XE, E

Pegto poroine clageiquo g . leg'valri.atlons edrologt{tree du

se ps# *,muns

r_ ffiqpgnffi f,e31snfAs6 .sesstr ae.t$.ss"rsqpgnf,s f,o3,l.crr$,s6; .Tgssi,nn'ti on"

tuffi"''fr,rsho Set,'dnuu,q p*usmmp bgT6. il r l

,, I

tr

CORTIUER, Oa

PERt, P. •

Jml'Z~, J.J •

Hog cholera Virus •acei:nation : comparis~:>n or immune

response in serum . .and in bucoopharyn,geal secretions.

Amt. Reoh. Vet. (B'<>us presse) 1976.

Porcine i~unoglobuline : structure and ~unction

Avtigem•li ty ot immunqgemoi ty : applie&:tion to the stud3 .. ' of ~he poTQine immime response against viral diseases.

Classical swine fever disease. Serological_ variation c.! the

virus in France and its role in the development o~ th~

disease :.n its sub-clinical or chronical form.

CEC Seminar OD "Porcine Immunology" Thi vervaJ.-Grignon,

17th-18th Septem~~~ 1973, (EUR'5450)

Characteristics ot the in vitro multiplication ot Bog

Cholera Virus.. 1, . ,

'!'ha in vitro PD•tic ID8Z'kera in relatioD to pathogem~ ty

to~ the pig.

Structure and s,rntpeaia ot imteotioua pancreatic Decra~ia

( IPlf) Vira.s RBA. · .

I'

Antigenic oomparispn ot swim fever virp (SFV) and M~osal

Disease virus (MDV;) ,,

CEC Seminar on "Studies on virus replication" !ruaaela,

2nd-4th Mq 1974 (EUR 5451)

Jl'liAtm, J.X. et LARENAUDIE, B.

.. .., ·'

Diagnosis or sub.,.clinical or atypical forma ot swine tever.

Demonstration of low virulent strains in tissue cultUl'- and

detection of meutr~i~ing antibodies. ! .

' t, '.

!. Characteriatica of a D!V ~i"e v1na vaoot• ap.iut &w,LM

:, ··I . tfi.Ver prep&l"ecl in ~illlll8 Cultwe at low teapel.'4L'twe. · •the TBIVIRVAL 8'\l'&i.D" I f'

r) '

. ...

.

I

•' '•' . -/ I

' )!. ,,

•'

,t,

..

' .• ~ i· '· t ~

t !t• , .. ~ ~ ' 1:

•J.,

gq,,wrt${*$rs e.ao.t# r'iE #fii mn**{Ea trf '*.$sog t}s -*su6ntJitl}1,

t.

"(ffi) wr1ns u1sod s.[T6p Bn;sTs ,iE s {ggfi} enT'*qg TBp 1__rr*.

$F#'$e ss.&frBFlp sTts'S tr{i.{Te Y.T "'sd.t gaspgorntmwE,11*6ddeg

"9 U$IN$IYA o"'d 'Itonra '"s. ofm{c

'"A lgU$UW'*;g omc6grsvo

..t":.

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,. '

ICoUfIJSVC - If,o[t8(,8rl lcuUllsil IUVXITIIITd^ 'Ill

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'gf 'ffiy1 Inra ,'0 tw,tuoo,n'il'f, *mrf,rr-?

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CAST'){ltGCI, C},. 11 !J!ToLI, P.,.RAmJCCI, Se C.AS'mO-!IORTUGAtf P .. L.o CILLI, V.,

P.!'f\"~"_}J't ' J·. St'tidy of the Experimental Infection of ·pip 1d ~Jl !oviDe

Viral Di8fl"hea (BVD) tfi~ .. loll .. Iet, Sieroter K;.lan~se, _u, 5 (1974) ..

Summar;y ot working gre>up 1 : ViroloQ' Em report •studj.es

on Yirua replieation•o 393-394 (EaR 5451).

'fAH lJIEU;;r~, J.Q., Impact Ot control programmes OD the epizootiology ot. the

disease in the tiel d .. EEC report •Diagnosis and epi zoo.­

t1ologr ot classical ewiM fever•, 211-229 (EUR 5486)

VJH ~, J .. a. Summary ot session 4, Impact ot control progrNUDes on the

epizootiology ot swi• tever u obs~rve4 i::& the ~ield,

254-255 (EUR 5486).

'l'ERPSTllA, o. Diagnosis ot claaeioal arviDe fever iD the Jletlwrlands,

58-63 (EUR 5486).

TERPSTRA0 c.. Smnmary of session 1. ConolMiODs u4 recommendation on

diagnostic aethoa fo'f' and.De fever, 251-252 (EOR 5486).,

'l'ERPSTRA, C. aDd VAN OIRSCH>T J. ;

Immunoeleotro-oemophoteaie tor detection ot antibody against swiM tner, 198-199 (D 5486). · .

rERPS'l'RA, C. u4 VAlJ OIRSCHOT J' •

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BilEAIlFKe X[,G" rlsbora,tory manrtq,l fon Feg€B,reh on .classical' aud a.frtc&llrwlarc fsrrerrc, Fq :60_6lu tfg?6) *" (ffifn 549?)_

mfrg[fiMs HoS,, ffiASS*#" alrd tAIIFS; It,Arsh^ €ps" Virusfotrseho .$,t, 3S6*31,S (ggtf)

ffiHfflffie t*G*r. s'as rl$t'g$Hr p*sa' r;ur*s fiH.E$$e sn*T*.

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Wrus an{'

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,t.

... , ' .. '.-.. 14-. ..

l XV~ DEPARTMEN'II OP ANIMAL HUSBANDRY - tmlYEl!SITY OF BRISTOL

BRADI.Er, P.A., BOURNE, P.J. and BROWN, P.J,

The respiratoey tract immune system in .. the Pig.

I. Distribution ot Iuulunoglobulin - oontainillg cells in

the respir•tor.y tract mucosa.

Vet. Patbol., !J.,. 81-89, 1976 •.

BRADL1ilT, . P.A. t !OURNE, P. J. and BROWN, P. J.

The respirator, traot immune system iD the Pig.

II. Associated ~phoid tiasuea.

Vet. Pathol., !J., '90-97, 1976.

H\llloral illlllluni ty in the Pi&'•

The Vetenna7 ReQord, June.19, 1976.

BROWB, P.J. ad JOURHE, P.J.

Distributions ot Immunoglobulin ... conta.inin« cella in

alimentar,y tract, spleen and mesenteric ~phnode ot the

Pig demoD~Strated by Peroxidase - conjugated aptiaeruaa to

' porcine immunoglobuliu a, .1. and. 11.

Alq6; J. Vet. Rea •. JI, 9-14, 1976.

',.

BROW, P.J. a1J4 JOURNE, P.J. · #

BBon, P.J.

Development ot i~unoglobulin - containing cell popula.tions

in the inteatine. 'spleen and mesenteric lymphnode or the

young pig deaonat~ated by peroxidase - conjugated antisera • . , ...

~ J. Vet. ·Rea., Press, 1976 A .

The imune system ~t the young pig

CEC Seminar on "Porcine Immunology" Thi'Verval-Grtgnon,

17th-18th September 1973 (EUR 5450) , '

Antigenic stimulation in the pig

CEC Semirmr on Studie~ on Virus replication- ~~eels,

~-4th Jlq 1974 (EUR 5451) to

r r

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' Ft'srymro*+Sei el3" ffieml"r,nm,r,*..t q$s {sl{'l\$id$,es *'m y,Lr,'u*a Hep},fgaut*ortw,

sr",rmeld** s*n$ bflmrs L$?d (:iprg} -- {ffir,rtt F4FL}-

90ffim,,3, Jil* wrbe &i"*4nom*m of s3as**oe3, s'or*no Fevcr sm $ped.nw"

Ppsmontmcm et s*mtmmn&ae sehro ffi.s$.a6r*os*m, nnd fut soCIt,tgls#ffef, #lasnfale] $ri.me Fmwerw, "smwtordemn ]$ ab.#"i.n *, *"trapb

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. fueeentndo aL $sn$,parso de EF"a.s"/B"n"G" E4pertr.cannnlta-sloa oa the Sradr'G*ttpu .sf &frtcnn amd Glasstael $trtnsFevorn" Hanoovenn 6*10 'de $opt$,enbro sa lgtg {Hn F4s?}

S&$effg?,-BOTIJA, 0. ,J ,,

ffi*frfcan Srriro Fovsno f, $a,pid Eie.6roete by fd.entificatlons:i Anti,bodtca Ertrpted nor tlreuse using tndlreg! iunUno-f.l:ioresc€trc€" rr" Da'ie tion of chrsnic fufectron audsa.rricr PtgE Pigs *i "{bnrrnoelebtnoooaphotegi sn?h*gcntado el Sour. ,qrlo rF.l.Or7t.E. C.. Erpert Coaenltationsa thc Eradloetjr ',r.F lfrlcarr and Clagslc4[ Srirs Fevers.Eamoygrr- 6-lfi Septtr otra fg?6 (gn 54g?).

rirt['i ',

nma goilzAwo, 4. r, trs?olEcurl t.ln and, 0nD[$, A.

trffrortbr "urlftortfoa aad Eractlonrtion df lfricen srt,r"kqgr V$ ngi

7

I '•

on the Eradication o~ African and Claaaical Swine Fever".

Bazmonr, 6-10 Septieabre: de 1976 (EUR 5487).· • I

SAJIC~BO'l'U.l, C., ORDAS, .&. , RUIZ GOIZALVO, P. and SOLANA, A.

• "ProcedUJ"ea i~ Use· tor Diagnosis ot African Swine Fever" •

P.reaentaclo &1 Seminpio "P.A.O./E.E.c. Experi Qopaula~iOD OD the Eradicat~~n ot Atrican and Classical Swi~ Pe,r•.

Balmonr, 6-10 Septieabre de 1976 (BUR 5487).

SAICBEZ-BO'l'IJ.l, C., 'l'ABAllES, P,, ORDAS, A., SOI.llll, .l., JllJIZ OOJZALVO, P.,

JWlCOIJ.SCO%, •• .l. :. co-authore.

Studies OD At'ri~ Swine Fever Virus : purification~

. i

'

t

uaal7aie of tM 'ri.riopa, to be pllbliebed (luR 5626) • \ ·

XVII,· LABORATORIO P.CIOlt\L Dm IWVESTIGACAO VETERilJARIA ... LISBOJNE ' r ~

\ -~ ·~.

R~plic99ao do ~rue da peate euina af'ric&D& (PSA) , '1 I

"in vivo" ~&D~lio Lintatico.

Xe Reurdao uau.l"d.a SOcieda4e Port'agaeaa 4a Kicroacopia

J:leotroDioa :. 12•13 Desembro 19'75.

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ANNEX ....'!!

FINANCIAl STATEM~T for t~e researph pro~ramme on Classical and African

Swine Fever

in UA

-------~-~~-~~---~~~--~~~--~~-~~~--~----~---~----~--~----~~~-~~-------------INSTITUTES : Funds availa­

ble in UA Funds used

(UA) . . % used : _,_ _____ "·"'"--------~~-.,-..-------~-~----------------_ .. ___ ... ________________ '1: ______ _

. • Hanovre 60 .. 328 . . 60.200 . . 100 . . -------·--~--~~~~---~---~--~~-~~-~~-------~~~-------------~~~~~--~---~--~--. . Tubtingen 60.656 43.531 72 ... -----------~-~-~-~---~----~--~~-~--~~----~~-----~------~------~~~~-----~---. . Uccle . . 257.520 . . 174.604 . . 68

----------~------~-~----~---~-~----~--~-------------~----------------------Alfort . . 79.224 . . 71.418 . . 90 . .

----------~-------------~-----~------------------~--~------~~------~--~---~ Thiver~l-Grignon 225.053 . . 216.413 .

• 96 . . -----~-------~-~-~----------~----~--------~---~-----------~------------------

Br.escia 100.454 87.588 87 . . ------~----------------------~~---~----------~-----------------------------. . Perugia : 91.36Q . . 90.560 99 . . --~-----------------------------~~~---~---------~~---------------------------. . Teramo . . 65.900 . . 57.201 . . 87 . • ----------------~-~----------------~------------------------~----------------. . Lelystad 110.995 85.695 . . 77 . . ------------------~--~-------~-----~----------~------~---~----------------Utrecht . . 35 .. 415 24.894 70 . . ---~--------~----~~----~--~----~~----~~-~~----------------~----~--~----------. . Madrid : 247.999 247.277 . . '•100

--------~---~---~~---------~~~-------------------------~-------------------­Lisbonne 0 . ,1

400.000. . . 231.949 58

------------------~-~---~--~~-----~--------------------------------------Dublin . . 102.,9~0 102.930 . . 100 . . --------------------------------------~--------------------~-----------------Pi rbright . . 302.203 131.276 . . 43 . . -~----------------~-~~------------·----------------------------------------Lindholm 132.000 . . 131.931 . . 100 . . ---------~---~----------~~----------~~---------------------------------------Bristol . . 109.095 105.165 . . 96 ---------------------~~----------~~--~-----------------------------------Weyhidge . . 69 •. 370 . . 48.924 . . 71 . . ---------~--------~-------~-------~-----~---~~~-------.---~-----------Coordination • • 272 •. 500 . . 236.672 . . 87 . . ---------------------------~-----~-----~--~~-------~~---------------• • TOTAL • • 2. 723,0.03 : • I • . . -----------------------~-·----------~~~----·------------------~---~--------

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