14
Comparison between GSR 1200 and GenePix 4000B GlycoTechnica Ltd.
Comparison between GSR 1200 and GenePix 4000B
GlycoTechnica Ltd.
GSR vs. GenePix
• FET, ASF and Cell Lysate were used as samples in this comparison
• (Background)BG signal intensities were adjusted at the same level to compare the sensitivity. In other words, we compared the performance emphasizing the importance of S/N ratios
• A conventional washing and Drying out protocol was used.
• As a result, it is clear that GSR has higher sensitivity than GenePix
ASF-2_GD_E199_G105_2gfedcb ASF-2_Gene_D_G380_2gfedcb
LTL 1
PSA 2
LCA 3
UEA_I
4
AO
L 5
AAL 6
MAL_I
7
SN
A 8
SSA 9
TJA
-I 1
0
PH
A(L
) 11
ECA 1
2
RCA120 1
3
PH
A(E
) 14
DSA 1
5
GSL-I
I 16
NPA 1
7
ConA 1
8
GN
A 1
9
HH
L 2
0
ACG
21
TxLC_I
22
BPL 2
3
TJA
-II
24
EEL 2
5
ABA 2
6
LEL 2
7
STL 2
8
UD
A 2
9
PW
M 3
0
Jacalin 3
1
PN
A 3
2
WFA 3
3
ACA 3
4
MPA 3
5
HPA 3
6
VVA 3
7
DBA 3
8
SBA 3
9
Cals
epa 4
0
PTL_I
41
MAH
42
WG
A 4
3
GSL_I_
A4 4
4
GSL_I_
B4 4
5
BG
Net
Inte
nsi
ty
42,000
40,000
38,000
36,000
34,000
32,000
30,000
28,000
26,000
24,000
22,000
20,000
18,000
16,000
14,000
12,000
10,000
8,000
6,000
4,000
2,000
0
■ASF GSR E199 G105 2well ■ASF GenePix G380 2well
E199 means exposure time by msec, G105 means Gain=105, 2well means No.2 Well on LecChip
FET_GD_E199_G105_6gfedcb FET_Gene_D_G380_6gfedcb
LTL 1
PSA 2
LCA 3
UEA_I
4
AO
L 5
AAL 6
MAL_I
7
SN
A 8
SSA 9
TJA
-I 1
0
PH
A(L
) 11
ECA 1
2
RCA120 1
3
PH
A(E
) 14
DSA 1
5
GSL-I
I 16
NPA 1
7
ConA 1
8
GN
A 1
9
HH
L 2
0
ACG
21
TxLC_I
22
BPL 2
3
TJA
-II
24
EEL 2
5
ABA 2
6
LEL 2
7
STL 2
8
UD
A 2
9
PW
M 3
0
Jacalin 3
1
PN
A 3
2
WFA 3
3
ACA 3
4
MPA 3
5
HPA 3
6
VVA 3
7
DBA 3
8
SBA 3
9
Cals
epa 4
0
PTL_I
41
MAH
42
WG
A 4
3
GSL_I_
A4 4
4
GSL_I_
B4 4
5
BG
Net
Inte
nsi
ty
50,000
48,000
46,000
44,000
42,000
40,000
38,000
36,000
34,000
32,000
30,000
28,000
26,000
24,000
22,000
20,000
18,000
16,000
14,000
12,000
10,000
8,000
6,000
4,000
2,000
0
■FET GSR E199 G105 6well ■FET GenePix G380 6well
CELL_GD_E199_G105_4gfedcb CELL_Gene_D_G380_4gfedcb
LTL 1
PSA 2
LCA 3
UEA_I
4
AO
L 5
AAL 6
MAL_I
7
SN
A 8
SSA 9
TJA
-I 1
0
PH
A(L
) 11
ECA 1
2
RCA120 1
3
PH
A(E
) 14
DSA 1
5
GSL-I
I 16
NPA 1
7
ConA 1
8
GN
A 1
9
HH
L 2
0
ACG
21
TxLC_I
22
BPL 2
3
TJA
-II
24
EEL 2
5
ABA 2
6
LEL 2
7
STL 2
8
UD
A 2
9
PW
M 3
0
Jacalin 3
1
PN
A 3
2
WFA 3
3
ACA 3
4
MPA 3
5
HPA 3
6
VVA 3
7
DBA 3
8
SBA 3
9
Cals
epa 4
0
PTL_I
41
MAH
42
WG
A 4
3
GSL_I_
A4 4
4
GSL_I_
B4 4
5
BG
Net
Inte
nsi
ty
75,000
70,000
65,000
60,000
55,000
50,000
45,000
40,000
35,000
30,000
25,000
20,000
15,000
10,000
5,000
0
■CELL GSR E199 G105 4well ■CELL GenePix G380 4well
Comparison between With Wash and Without Wash
• GenePix can not take fluorescence images directly from a liquid phase without washing and drying up the slide glass surface. GSR is the only scanner which is able to detect fluorescence signals without washing.
• Two gains were used, 105 and 125.
• The same sample set was used in this comparison.
• It is clearly shown that the signal intensities drops with washing, especially for weak interactions. In other words, GSR has higher performance in terms of qualitative measurement.
Cont. • Multivalent interactions could reduce the
decrease of signal intensities due to the washing. ASF and FET have multivalent interactions.
• A paper written by Uchiyama et al. using LecChips and GSR 1200 must be a good paper in this point. Proteomics 2008, 8, pp3042-3050,
http://www.ncbi.nlm.nih.gov/pubmed/18615430
• Totally speaking, it is clear that GSR 1200 is the best scanner to monitor weak Lectin-Glycan interactions.
ASF-2_GD_E199_G105_2gfedcb ASF-2_GWet_E199_G105_2gfedcb
LTL 1
PSA 2
LCA 3
UEA_I
4
AO
L 5
AAL 6
MAL_I
7
SN
A 8
SSA 9
TJA
-I 1
0
PH
A(L
) 11
ECA 1
2
RCA120 1
3
PH
A(E
) 14
DSA 1
5
GSL-I
I 16
NPA 1
7
ConA 1
8
GN
A 1
9
HH
L 2
0
ACG
21
TxLC_I
22
BPL 2
3
TJA
-II
24
EEL 2
5
ABA 2
6
LEL 2
7
STL 2
8
UD
A 2
9
PW
M 3
0
Jacalin 3
1
PN
A 3
2
WFA 3
3
ACA 3
4
MPA 3
5
HPA 3
6
VVA 3
7
DBA 3
8
SBA 3
9
Cals
epa 4
0
PTL_I
41
MAH
42
WG
A 4
3
GSL_I_
A4 4
4
GSL_I_
B4 4
5
BG
Net
Inte
nsi
ty
42,000
40,000
38,000
36,000
34,000
32,000
30,000
28,000
26,000
24,000
22,000
20,000
18,000
16,000
14,000
12,000
10,000
8,000
6,000
4,000
2,000
0
■ASF GSR Dry E199 G105 2well ■ASF GSR Wet E199 G105 2well
ASF-2_GD_E199_G125_2gfedcb ASF-2_GWet_E199_G125_2gfedcb
LTL 1
PSA 2
LCA 3
UEA_I
4
AO
L 5
AAL 6
MAL_I
7
SN
A 8
SSA 9
TJA
-I 1
0
PH
A(L
) 11
ECA 1
2
RCA120 1
3
PH
A(E
) 14
DSA 1
5
GSL-I
I 16
NPA 1
7
ConA 1
8
GN
A 1
9
HH
L 2
0
ACG
21
TxLC_I
22
BPL 2
3
TJA
-II
24
EEL 2
5
ABA 2
6
LEL 2
7
STL 2
8
UD
A 2
9
PW
M 3
0
Jacalin 3
1
PN
A 3
2
WFA 3
3
ACA 3
4
MPA 3
5
HPA 3
6
VVA 3
7
DBA 3
8
SBA 3
9
Cals
epa 4
0
PTL_I
41
MAH
42
WG
A 4
3
GSL_I_
A4 4
4
GSL_I_
B4 4
5
BG
Net
Inte
nsi
ty
80,000
75,000
70,000
65,000
60,000
55,000
50,000
45,000
40,000
35,000
30,000
25,000
20,000
15,000
10,000
5,000
0
■ASF GSR Dry E199 G125 2well ■ASF GSR Wet E199 G125 2well
FET_GD_E199_G105_6gfedcb FET_GWet_E199_G105_6gfedcb
LTL 1
PSA 2
LCA 3
UEA_I
4
AO
L 5
AAL 6
MAL_I
7
SN
A 8
SSA 9
TJA
-I 1
0
PH
A(L
) 11
ECA 1
2
RCA120 1
3
PH
A(E
) 14
DSA 1
5
GSL-I
I 16
NPA 1
7
ConA 1
8
GN
A 1
9
HH
L 2
0
ACG
21
TxLC_I
22
BPL 2
3
TJA
-II
24
EEL 2
5
ABA 2
6
LEL 2
7
STL 2
8
UD
A 2
9
PW
M 3
0
Jacalin 3
1
PN
A 3
2
WFA 3
3
ACA 3
4
MPA 3
5
HPA 3
6
VVA 3
7
DBA 3
8
SBA 3
9
Cals
epa 4
0
PTL_I
41
MAH
42
WG
A 4
3
GSL_I_
A4 4
4
GSL_I_
B4 4
5
BG
Net
Inte
nsi
ty
55,000
50,000
45,000
40,000
35,000
30,000
25,000
20,000
15,000
10,000
5,000
0
■FET GSR Dry E199 G105 6well ■FET GSR Wet E199 G105 6well
FET_GD_E199_G125_6gfedcb FET_GWet_E199_G125_6gfedcb
LTL 1
PSA 2
LCA 3
UEA_I
4
AO
L 5
AAL 6
MAL_I
7
SN
A 8
SSA 9
TJA
-I 1
0
PH
A(L
) 11
ECA 1
2
RCA120 1
3
PH
A(E
) 14
DSA 1
5
GSL-I
I 16
NPA 1
7
ConA 1
8
GN
A 1
9
HH
L 2
0
ACG
21
TxLC_I
22
BPL 2
3
TJA
-II
24
EEL 2
5
ABA 2
6
LEL 2
7
STL 2
8
UD
A 2
9
PW
M 3
0
Jacalin 3
1
PN
A 3
2
WFA 3
3
ACA 3
4
MPA 3
5
HPA 3
6
VVA 3
7
DBA 3
8
SBA 3
9
Cals
epa 4
0
PTL_I
41
MAH
42
WG
A 4
3
GSL_I_
A4 4
4
GSL_I_
B4 4
5
BG
Net
Inte
nsi
ty
80,000
75,000
70,000
65,000
60,000
55,000
50,000
45,000
40,000
35,000
30,000
25,000
20,000
15,000
10,000
5,000
0
■FET GSR Dry E199 G125 6well ■FET GSR Wet E199 G125 6well
CELL_GD_E199_G105_4gfedcb CELL_GWet_E199_G105_4gfedcb
LTL 1
PSA 2
LCA 3
UEA_I
4
AO
L 5
AAL 6
MAL_I
7
SN
A 8
SSA 9
TJA
-I 1
0
PH
A(L
) 11
ECA 1
2
RCA120 1
3
PH
A(E
) 14
DSA 1
5
GSL-I
I 16
NPA 1
7
ConA 1
8
GN
A 1
9
HH
L 2
0
ACG
21
TxLC_I
22
BPL 2
3
TJA
-II
24
EEL 2
5
ABA 2
6
LEL 2
7
STL 2
8
UD
A 2
9
PW
M 3
0
Jacalin 3
1
PN
A 3
2
WFA 3
3
ACA 3
4
MPA 3
5
HPA 3
6
VVA 3
7
DBA 3
8
SBA 3
9
Cals
epa 4
0
PTL_I
41
MAH
42
WG
A 4
3
GSL_I_
A4 4
4
GSL_I_
B4 4
5
BG
Net
Inte
nsi
ty
75,000
70,000
65,000
60,000
55,000
50,000
45,000
40,000
35,000
30,000
25,000
20,000
15,000
10,000
5,000
0
■CELL GSR Dry E199 G105 4well ■CELL GSR Wet E199 G105 4well
CELL_GD_E199_G125_4gfedcb CELL_GWet_E199_G125_4gfedcb
LTL 1
PSA 2
LCA 3
UEA_I
4
AO
L 5
AAL 6
MAL_I
7
SN
A 8
SSA 9
TJA
-I 1
0
PH
A(L
) 11
ECA 1
2
RCA120 1
3
PH
A(E
) 14
DSA 1
5
GSL-I
I 16
NPA 1
7
ConA 1
8
GN
A 1
9
HH
L 2
0
ACG
21
TxLC_I
22
BPL 2
3
TJA
-II
24
EEL 2
5
ABA 2
6
LEL 2
7
STL 2
8
UD
A 2
9
PW
M 3
0
Jacalin 3
1
PN
A 3
2
WFA 3
3
ACA 3
4
MPA 3
5
HPA 3
6
VVA 3
7
DBA 3
8
SBA 3
9
Cals
epa 4
0
PTL_I
41
MAH
42
WG
A 4
3
GSL_I_
A4 4
4
GSL_I_
B4 4
5
BG
Net
Inte
nsi
ty
80,000
75,000
70,000
65,000
60,000
55,000
50,000
45,000
40,000
35,000
30,000
25,000
20,000
15,000
10,000
5,000
0
■CELL GSR Dry E199 G125 4well ■CELL GSR Wet E199 G125 4well
