Complete manual 2.0 -...

MIDAS

Autosampler SERVICE MANUAL

Part no. 0060.160-2.0

P.O. BOX 388,

7800 AJ EMMEN,

THE NETHERLANDS

Phone: (31) 591 - 631700

Fax: (31) 591 – 630035

E-mail: [email protected]

http://www.sparkholland.com

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Table of contents IMPORTANT NOTICE ........................................................................................... 4 SAFETY REMINDERS ........................................................................................... 4

MECHANICAL SAFETY ......................................................................................... 6 ELECTRICAL SAFETY ........................................................................................... 7 1 PROGRAMMING CHART ................................................................................11 2 I/O CONNECTIONS. .....................................................................................12 3 SERVICE MODE.............................................................................................15 3.1 TRAY .....................................................................................................17 3.2 NEEDLE .................................................................................................18 3.3 TEMPERATURE CONTROL .........................................................................20 3.4 SYRINGE................................................................................................21 3.5 INJECTOR VALVE ....................................................................................22 3.6 EXTERNAL I/O ........................................................................................23 3.7 SERIAL PORT..........................................................................................24 3.8 PIERCE TEST ..........................................................................................25 3.9 LIFE TEST ..............................................................................................26 3.10 RESET LOG COUNTERS ............................................................................27

4 DISASSEMBLING ..........................................................................................28 4.1 REMOVING THE TOP COVER .....................................................................29 4.2 INJECTION VALVE ...................................................................................30 4.2.1 Replacing the Injection Valve ................................................................30 4.2.2 Replacing the Valve drive assembly........................................................31 4.2.3 Maintenance for Valco C2-2006/2346 valve.............................................32 4.2.4 Maintenance on the Rheodyne Model 7739 valve .....................................33 4.2.5 Maintenance on the Rheodyne Model 9740 valve .....................................34

4.3 DISPENSER ............................................................................................35 4.3.1 Removing the dispenser .......................................................................35 4.3.2 Dispenser valve ...................................................................................36

5 ADJUSTMENTS .............................................................................................37 5.1 NEEDLE HEIGHT ADJUSTMENT..................................................................37 5.2 SYRINGE HOME ADJUSTMENT...................................................................38 5.3 PENETRATION POINT ADJUSTMENT...........................................................39 5.3.1 Left Right adjustment.......................................................................39 5.3.2 Front Back adjustment ......................................................................40

5.4 VIAL STRIPPER ADJUSTMENT ...................................................................41 5.5 VIAL SENSOR ADJUSTMENT .....................................................................42

6 FIRMWARE REPLACEMENT ...........................................................................43 7 TROUBLE SHOOTING ....................................................................................44 7.1 ERROR CODES........................................................................................44 7.2 ANALYTICAL ...........................................................................................51

8 NEEDLE ........................................................................................................54 8.1 NEEDLE CONNECTIONS ...........................................................................54

9 PELTIER COOLING OPTION ..........................................................................55 9.1 PELTIER ELEMENT REPLACEMENT..............................................................56

10 LIST OF ACCESSORIES.............................................................................57 11 TEST PROCEDURE ....................................................................................59 Exploded view drawings and schematics ...........................................................63 0830.302 Valve drive assembly parts list ...........................................................64 Exploded view of Valve drive assembly ..............................................................65

0900.302 Syringe Dispenser assembly parts list ....................................................66 Exploded view of Syringe dispenser assembly.....................................................67

0830.305 Mains inlet assy 0830 parts list .............................................................68 Exploded view of mains inlet assy 0830 .............................................................69

0830.307 Needle drive assy 0830 parts list ...........................................................70 Exploded view of needle drive assy ...................................................................71

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0830.308 Needle arm assy 0830 parts list ............................................................72 Exploded view of Needle arm assy 0830 ............................................................73

0830.310 Oven assy 0830 parts list .....................................................................74 Exploded view of Oven assy 0830 .....................................................................75

0830.320 Keyboard cover assy 0830 parts list.......................................................76 Exploded view of keyboard cover assy 0830.......................................................77

0830.401 Bottom frame assy 0830 parts list .........................................................78 Exploded view of Bottom frame assy 0830.401...................................................79 Exploded view of Bottom frame assy (top view)..................................................80

0830.402 Mainframe assy 0830 parts list..............................................................82 Exploded view of Mainframe.............................................................................83

0830.403 Rearpanel assy 0830 parts list ..............................................................84 Exploded view Rearpanel 0830 .........................................................................85

0830.840 Cooling option 0830 parts list................................................................86 Exploded view Cooling option 0830 ...................................................................87

Electronic drawings and schematics ..................................................................88 0830.601 CPU PCB Component view of mainboard ..............................................89 0830.601 Electronic diagram mainboard ............................................................90

Tray needle Sensor PCB 0830.602 comp. view ......................................................91 Keyboard PCB 0830.603 ..................................................................................92

Multilink serial interface PCB 0725.625 .................................................................93

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IMPORTANT NOTICE

This MIDAS Service Manual is designed for use by personnel who have had training and

are experienced in servicing this type of equipment. Because of the possible hazards to

an inexperienced person in servicing this product, as well as the risk of damage to the

instrument, we strongly recommend that all servicing be performed by our Field Service

Representatives.

In addition, to improve instrument performance, changes have been made to the

instrument by us after this MIDAS Service Manual was printed. Accordingly, we make no

representations or warranties, either express of implied, that the information contained in

this MIDAS Service Manual is complete or accurate. It is understood that the purchaser

must assume all risk in the use of this Manual for the purpose of performing service upon

the instrument it covers.

Components of this instrument which are considered user-serviceable are discussed in

detail in the instrument’s Users Manual.

Spark Holland strongly recommends to use only original Spark Holland spare parts,

otherwise we do not guarantee any specification or liabilities.

SAFETY REMINDERS

The following pages summarise cautionary information basic to the safe operation of this

instrument. These safety hazards are indexed by page number in order of their

appearance in the manual. However, it is strongly recommended that the user reads the

entire manual carefully before attempting to service the instrument. In addition, be sure

to heed all, DANGER, WARNING, CAUTION, ATTENTION and NOTE signs and

pictograms which are specifically defined as follows:

DANGER:

The "DANGER sign" denotes a hazard. It calls attention to a

procedure, practice or the like, which, if not correctly done or

adhered to, could results in injury or loss of life.

Do not proceed beyond a "DANGER sign" until the indicated

conditions are fully understood and met.

WARNING

WARNING:

The "WARNING sign" denotes a hazard. It calls attention to a


adhered to, could result in severe injury or damage or

destruction of parts or all of the equipment.

Do not proceed beyond a "WARNING sign" until the indicated


CAUTION

CAUTION:

The "CAUTION sign" denotes a hazard. It calls attention to a


adhered to, could result in damage or destruction of parts or all

of the equipment.

Do not proceed beyond a "CAUTION sign" until the indicated


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ATTENTION:

The "ATTENTION sign" denotes relevant information.

Read this information first, it might be helpful or necessary

before continuing.

NOTE:

The "NOTE sign" denotes additional information.

It provides the user with advises and suggestions to ease the

use of the equipment.

ATTENTION:

Observe precautions for handling Electrostatic discharge

sensitive devices.

The following safety practices are intended to insure the safe operation of the equipment.

Electrical Hazards

1. Removal of some panels exposes potentially dangerous voltages.

Disconnect the instrument from all power sources before removing

protective panels.

2. Replace blown fuses with size and rating stipulated on the fuse panel or

holder and in the manual where listed.

3. Replace or repair faulty or frayed insulation on power cords.

4. Check actual line voltage to confirm it is the value for which this instrument

is wired. Be sure power cords are plugged into correct voltage sources.

General Precautions

1. Perform periodic leak checks on supply lines.

2. Do not allow flammable and/or toxic solvents to accumulate. Follow a

regulated, approved waste disposal program. Never dispose of such

products through the municipal sewage system.

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MECHANICAL SAFETY

Good mechanical safety practices are more important than ever, now that

microprocessors are taking control of mechanical devices. Yet, the area of mechanical

safety is often overlooked during instrument servicing. We may be able to change this

situation if you heed the following safety tips:

1. Remember to keep clothing and fingers away from rotating components. It is easy to

slip, lose a tie, or get cut while working near one of these devices.

2. Unless it is absolutely essential, never run mechanical components without the

protective guards in place.

3. Always use the tools specifically recommended for the job. This not only reduces the

chances of injury, it minimises damage to the instrument.

4. Although not always possible, it is recommended that mechanical devices should be

adjusted or calibrated with the power OFF. This is especially important if you are

working with a microprocessor-controlled instrument, which can start running without

notice or user-intervention.

5. After the mechanical repairs have been completed, always check that all mounting

hardware and safety shields are in place and secure. By doing so you will not only

ensure safe operation of the instrument, but may reduce call-backs.

As you can appreciate, all it takes is good common sense, plus good safety techniques

that are basic and easy to remember. With this in mind, you should have no trouble

servicing mechanical devices safely

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ELECTRICAL SAFETY

It is shocking but true! Many people service instruments without giving much thought to

electrical safety. Unfortunately, this can be a dangerous mistake. As electrical/electronic

devices are used more widely in modern instrumentation, it means that you may be

exposed to more and more potential shock hazards.

It pays to take a few precautions before and during servicing to avoid injury from

electrical shock. Here are a few safety tips that can help keep you on the job without a

sudden vacation:

1 Avoid standing on damp floors! People have been shocked simply by standing on a

damp floor and working on live equipment.

2 Be sure to disconnect the power cord before working on any high voltage circuitry! As

power switches disappear from new equipment, this precaution is very easy to

overlook.

3 Read and heed ALL caution labels! They are posted for your safety.

4 Take care of your test equipment. Be sure to use the right probes for the right job.

Measuring high voltage, for example, requires a well insulated high-voltage probe.

Damaged probes and cables are dangerous and should not be used. Also, be cautious

around test equipment like an oscilloscope. The oscilloscope case may become live if

you connect the cable ground to a live circuit. Avoid this dangerous situation at all

times!

The basic tips regarding good electrical safety practices are easy to remember.

Combined with good common sense, they should keep you on the job for a long time to

come.

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SPECIFICATIONS

GENERAL

Sound pressure level <70 dB

Working temperature 10 - 40°C

Installation category Category II, according to IEC 1010

Pollution degree Degree 2, according to IEC 1010

SAMPLING

Sample capacity: Standard tray:

84 vials of 1.5 ml (Std) and

3 vials of 10 ml (LSV)

Vial dimensions (cap

included):

Maximum vial height: 47 mm

Minimum vial height: 32 mm

Loop volume 1 - 5000 µl

Dispenser syringe 250 µl (standard) or 1000 µl syringe

Vial detection Missing vial detection by vial sensor.

Sample vial access <10 seconds

Headspace pressure Built-in compressor

Switching time injection

valve

Electrically <100 msec

Piercing precision needle ± 0.6 mm

Wash solvent External wash solvent bottle

Wetted parts SS316, PTFE, TEFZEL®, VESPEL®, Teflon.

Optional: PEEK

ANALYTICAL PERFORMANCE

Reproducibility RSD <= 0.3% for full loop injections

RSD <= 0.5% for partial loopfill injections,

injection volumes >10µl

RSD <= 1.0% for µl pick-up injections, injection

volumes >10µl

Memory effect < 0.05% with programmable needle wash

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Column oven Built-in column thermostat

Range: (Tambient +5) - 60°C

Accuracy ± 1°C (measured in the

centre)

Dimensions:

(W x D x L)

25 mm x 25 mm x 300 mm

1inch x 1 inch x 11.8 inch

PROGRAMMING

Methods 9 programmable methods

Injection item in method Injection mode:

Full loop injection

Partial loopfill injection

µl pick-up injection

Injection volume 1 µl - 5000 µl, with 1 µl increments

Injections per vial max. 9 injections

Analysis time max. 9 hr 59 min 59 sec

Wash item Programmable;

Wash between injections

Wash between vials

Wash between series

Mix item max. 15 programmable steps

Series 9 programmable series

Priority sample Programmable

PHYSICAL

Dimensions (W x D x H) 300 mm x 500 mm x 340 mm

11.8 in. x 19.7 in x 13.4 in

Weight 19 kg (42 lb.)

21 kg (46 lb.) with cool option

ELECTRICAL

Power requirements 115/230 VAC; +15%/-22%; 50 Hz / 60 Hz; 200

VA

Fuses For 115 VAC; two 5.0 AT-fuses

(slow, ¼” x 1¼”, UL/CSA)

For 230 VAC; two 2.5 AT-fuses

(slow, 5 x 20 mm, IEC 127)

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COMMUNICATION

Outputs Inject marker (Relay & TTL)

2 Auxiliary outputs (Relay)

Alarm output (Relay)

Inputs Next injection input (TTL)

Freeze input (TTL)

Stop input (TTL)

Serial communication port RS232C

OPTIONS (User installable)

Large capacity tray: 96 vials of 1.5 ml (STD, 12 mm OD)

Large volume tray: 24 vials of 10 ml (LSV, 22 mm OD)

PC Control: SparkLink or SparkLink Lite

Microsoft® Windows™ oriented software

package.

Including installed interface software, application

running under Windows XP.

OPTIONS (Factory installed)

Sample tray cooling Built-in Peltier cooling

Range: 4°C - 15°C

Accuracy: ± 2°C (at temperature

sensor)

(Temperature at relative humidity of 60%)

Cooling capacity: 20°C below ambient.

Bio-compatible valve Valco or Rheodyne special PEEK injection valve,

quick connect mounting.

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1 PROGRAMMING CHART

Ready screen <WASH> <MAINTENA

NCE>

<UTILS> <SERIAL

>

<TEMP> <SERVICE>

<COPY> <ERASE> <LOG> <OVEN> <TRAY COOL>1

Perform standard wash

Ndle Hor Move syringe Switch valve

Copy methods

Erase methods

Midas logbook

Serial communica

tion protocol

Control column oven

temperature

Control sample tray cooling

Service mode

[System] System Settings

<GENERAL> <METHOD> <TRAY> <IO> <SERIAL>

Loop volume

Needle volume Syringe volume

Syringe speed Skip missing vials

Air segment Headspace pressure

Use Timed events

Use Mix Use calibration vials

Tray type Inject marker pulse

length Input edge next

injection Freeze input active

Reset outputs after last series

Device identifier

[Methods] Method programming

<INJECTION> <WASH> <TIMED EV> <MIX>

Injection type:

<FULL> <PARTIAL> <PICK-UP>

Wash volume Timed events program:

Mix program:

Flush volume

No injections/vial Analysis time

Flush volume

No injections/vial Injection volume

Analysis time

No

injections/vial Injection volume Analysis time

Wash

between: -

Series -

Vials - Series

- 4 x AUX-1

- 4 x AUX-2

- INITIAL OVEN SETPOINT

- 3 x

OVENSETPOINT - END TIME

- Add

- Mix - Wait

[Series]

Method number

First sample vial Last sample vial

Use calibration vials2 First calibration vial

Last calibration vial Vials between calibration

First destination vial3

[Start/Stop] First Series

Last Series

<START> <REMOTE>

[Priority] Sample vial

[Hold/Cont.]

Hold the analysis time of the Midas

1 Optional

2 Depends on the System Settings.

3 In case Destination vials are used in the mix item.

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2 I/O CONNECTIONS.

The Midas has standard three I/O connectors:

1. TTL input connector (P2)

2. Contact closures output connector (P1)

3. Communication port (3)

WARNING

The manufacturer will not accept any liability for damages directly

or indirectly caused by connecting this machine to instruments

which do not meet relevant safety standard.

Contact closure output connector (P1):

Pin

no:

Description: Pin

no:

Description:

1 Inject marker 1 - Normally

open

10 Alarm output - Normally

open

2 Inject marker 1 - Common 11 Alarm output - Common


closed

12 Alarm output - Normally

closed

4 Auxiliary 1 – Normally open 13 Auxiliary 2 - Normally open

5 Auxiliary 1 – Common 14 Auxiliary 2 - Common

6 Auxiliary 1 – Normally closed 15 Auxiliary 2 - Normally closed


open

8 Inject marker 2 - Common


closed

Contact closure VMAX = 150 VDC /125 VAC , IMAX = 1 A

An Inject marker output (1 and 2) will be generated when the injection valve switches

from LOAD to INJECT. The Alarm Output will be activated whenever an error occurs,

see Error Code list for a description of the error codes of the Midas.

Common (com)

Normally open

Normally closed

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TTL input connector (P2):

This connector is an active high or active low TTL input, user definable in the System

Settings. The Next Injection Input can be used when the Midas works in Remote

Control and in Ready mode. The Freeze Input and Stop Input can be used to control

the Midas by other devices.

Connector P2: TTL inputs.

Pin

no:

Description:

1 Next Injection

Input

2 Signal Ground

3 Freeze Input

4 Stop Input

5-9 Signal Ground

Autosampler Autosampler

Next Injection Input:

This input will start the next injection sequence when the Midas is started in Remote

Control. After finishing the injection sequence the Midas will wait for the Next

Injection Input.

From the Ready screen a Next Injection Input will start the latest programmed

series. In this case the Midas will not wait for the Next Injection Input before

continuing with the next injection. The Midas will execute the complete programmed

series as if it was started with the [Start/Stop] key.

Freeze input:

The Midas will freeze the analysis time for the time this input is active. If the Freeze

Input is activated while the analysis time is not running, the Midas will perform all

programmed pre-injection sample handling (mix method and loading part of the

injection method). But the Midas will wait with injecting the sample until the Freeze

Input is no longer active.

Stop Input:

With this input the run of the Midas is immediately aborted and returns to the Ready

screen. In case the Midas is in Remote Control, the Run of the Midas is immediately

aborted but the Midas remains in Remote Control and can not be re-started with a

Next Injection Input.

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Communication port (P3):

The Midas is standard equipped with Multilink male-female 9 pins D-connector for serial

communication. This interface allows you to connect your Midas with other instruments,

like Prospekt 2 , Mistral Column oven and PC.

To achieve communication between the Midas and the Software on the PC the Device

identifier should be set at the same value.

The identifier of the Midas is programmable from 60 to 69. To change the Device

Identifier, press: SYSTEM, MENU and <SERIAL>

To use the serial communication press MENU and <SERIAL>.

The pin connections of the 9 pins D-connectors are as follows:

Port OUT (9 pins D-connector male) Port IN (9 pins D-connector

female)

Pin no. Description Pin no. Description

2 Rx-2 2 Tx-1

3 Tx-2 3 Rx-1

4 Valve_out 4 Valve_in

5 Gnd 5 Gnd

6 AS_ready_in 6 AS_ready_out

7 Downstream_sync_2 7 Downstream_sync_1

8 Upstream_sync_2 8 Upstream_sync_1

NOTE:

The above described multilink PCB is the standard

communication PCB.

Optional other interfaces like RS422 can be found on different

OEM versions.

• Set the dipswitch S1 and S2 as follows: S1 1 2 3 4

ON

OFF

S2 1 2 3 4

ON

OFF

Port Description

Out To next device

In To PC or Prospekt 2

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3 SERVICE MODE The software of the Midas is provided with a service mode.

In the service mode it is possible to control all the outputs, mechanical movements and

to require information from all the inputs.

Since the service mode is only to be used by a qualified trained service engineer it is

protected by a code.

To enter the service mode, from the READY screen, press [MENU] followed by the

softkey <SERVICE>. Then enter the service code: 101163.

The service mode is set up in such a way that each function of the unit has got its own

pages.

To enter a required function; press the corresponding softkey.

To enter the Service Mode proceed as follow:

Power up the unit

MIDAS [MENU]

READY (X.XX) OVEN:--°°°°C

TRAY:--°°°°C <WASH> <MAINTENANCE> <SERIAL> <TEMP>

Press: [MENU]

MIDAS [MENU]

READY (X.XX) OVEN:--°°°°C

TRAY:--°°°°C <SERVICE>

Press: <SERVICE>

ENTER SERVICE-CODE, THEN PRESS ENTER

Press: 1 0 1 1 6 3[E]

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After entering the service code the following screen will be shown.

SERVICE MODE [MENU]

<TRAY> <NEEDLE> <TEMP> <SYRINGE>

On the lower line the function of each soft key is displayed.

Representing the different function/assembly in the system.

To change the function of soft key’s press [MENU] button.

SERVICE MODE [MENU]

<INJ VALVE> <EXT IO> <SERIAL> <PIERCE>

Press [MENU] button again for the following page.

SERVICE MODE [MENU]

<LIFE TEST> <RESET LOG>

For a detailed description for each function (sub assembly) check the following

paragraphs.

CAUTION

CAUTION:

Not all the safety precautions which are imbedded in the

normal run mode will function in the service mode.

Meaning that it might be possible, for instance, to rotate

the tray while the needle is down.

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3.1 TRAY

SERVICE MODE

TRAY SEARCH VIAL:XX

SENSORS HOME:X POS:X TYPE:84+3

<START>

Check first if the type of tray which is installed on the autosampler is identical to the

displayed version

SEARCH VIAL : A value can be entered to which the tray will search

Press the soft key START to activate the search.

SENSORS

HOME: X : Indicates the status of the HOME sensor.

This sensor is used to recognize the vial 01 position.

= 0 Sensor is not interrupted by the tray disc.

= 1 Sensor is interrupted by the tray disc.

POS: X : Indicates the status of the POSITION sensor.

This sensor is used to stop the tray at the correct position.

POS: 0

= 0 Sensor is not interrupted by the tray disc

= 1 Sensor is interrupted by the tray disc.

SOFTKEY

<START> : After pressing the softkey <START> the tray will search for the

position entered in the search parameter.

Press <ESCAPE> to return to the main menu of the Service Mode.

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3.2 NEEDLE

The needle page is set up in 3 different pages use [MENU] to scroll through the

different pages.

SERVICE MODE [MENU]

NEEDLE VERTICAL

SENSORS UP:X DOWN:X VIAL:X

<HOME> <DOWN>

SENSORS

UP: X : Indicates the status of the needle up sensor.

This sensor is used to check if the needle arm is completely in the

upper position.

= 0 Needle arm is not completely up.

= 1 Needle arm is completely up.

DOWN: X : Indicates the status of the needle up sensor.

This sensor is used to check if the needle arm is completely down.

= 0 Needle arm is not completely down.

= 1 Needle arm is completely down.

VIAL: X : Indicates the status of the vial sensor.

This sensor is used to check if there is a vial placed in the tray at

the position the Autosampler is searching for.

= 0 Vial is detected by the vial sensor.

= 1 No vial detected by the vial sensor.

SOFTKEYS

<HOME> Moves the needle arm up to its most upper position.

<DOWN> Moves the needle arm down to its most lowest position

NOTE:

It is only possible to move the needle arm down on a legal tray

position which is safe for the needle.

Else ERROR 237 will be displayed

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SERVICE MODE [MENU]

NEEDLE HORIZONTAL

SENSORS UP:X LEFT/RIGHT:X WASH:X

<HOME> <NEXT> <PREVIOUS>

SENSORS

UP: X : Indicates the status of the needle up sensor.

This sensor is used to check if the needle arm is completely in the

upper position.

= 0 Needle arm is not completely up.

= 1 Needle arm is completely up.

LEFT/RIGHT: X Indicates the status of the left/right sensor.

This sensor is used to check if the needle arm is above a correct

position of the tray.

= 0 Needle arm is above a correct position of the tray.

= 1 Needle arm is not above a correct position of the tray.

WASH: X Indicates the status of the wash position sensor.

This sensor is used to check if the needle arm is above the wash

position.

= 0 Needle arm is not above the wash position.

= 1 Needle arm is above the wash position.

SOFTKEYS

<HOME> : Moves the needle arm to the home position above the wash

position.

<NEXT> Moves the needle arm to the next horizontal position

<PREVIOUS> Moves the needle arm to the previous horizontal position.

NOTE:

It is only possible to move the needle arm horizontal if the

needle up sensor = 0

Else ERROR 234 will be the result

SERVICE MODE [MENU]

NEEDLE COMPRESSOR

<ON> <OFF>

SOFTKEYS

<ON> : Turns the compressor ON

<OFF> : Turns the compressor OFF

20

3.3 TEMPERATURE CONTROL

OVEN CONTROL: SERVICE MODE

TEMP CONTROL OVEN SETPOINT:XX°C

ACTUAL: XX°C XX COUNTS XXXmV POWER:---%

<ON> <OFF>

SETPOINT: XX°C : The SETPOINT can be changed with the numeric keypad.

ACTUAL: XX°C : Current temperature of oven compartment.

XX COUNTS : Output from the AD converter.

XXX mV : Output voltage from the oven sensor.

POWER: ---% : indicates the amount of power used by the heating element.

SOFTKEYS

<ON> : Turns the oven ON

<OFF> : Turns the oven OFF

COOLING CONTROL (OPTIONAL): SERVICE MODE [MENU]

TEMP CONTROL COOLING SETPOINT:XX°C

ACTUAL: XX°C XX COUNTS XXXmV POWER:---%

<ON> <OFF>

SETPOINT: XX°C : The SETPOINT can be changed with the numeric keypad.

ACTUAL: XX°C : Current temperature of the cooling ring.

XX COUNTS : Output from the AD converter.

(depends on the actual temperature

Power:---% : Indicates the amount of power used by the heating element.

SOFTKEYS

<ON> : Turns the cooling ON

<OFF> : Turns the cooling OFF

21

3.4 SYRINGE SERVICE MODE [MENU]

SYRINGE

SENSORS HOME:X ROTATION:X

<HOME> <END>

SENSORS

HOME: X : Indicates the status of the home sensor.

This sensor is used to check if the syringe is at the home position.

= 0 Sensor vane is not in the home sensor.

= 1 Sensor vane is in the home sensor.

ROTATION: X : Indicates the status of the rotation sensor.

This sensor is used to check if the syringe spindle rotates

without obstructions

= 0 Slot of rotation disc is inside sensor

= 1 Rotation disc is inside sensor

SOFTKEYS

<HOME> : Resets the syringe to the home position

<END> : Moves the syringe completely down.

SERVICE MODE [MENU]

SYRINGE VALVE

SENSORS LEFT:X RIGHT:X

<WASH> <NEEDLE> <WASTE>

SENSORS

LEFT: X: Indicates the status of the left syringe valve sensor.

= 0 Slot of rotation plate is inside sensor.

= 1 Rotation plate is inside sensor.

RIGHT: X : Indicates the status of the right syringe valve sensor.

= 0 Slot of rotation plate is inside sensor.

= 1 Rotation plate is inside sensor.

SOFTKEYS

Sensor displays: LEFT

RIGHT

<WASH> : Sets the syringe valve in wash position 0 1

<NEEDLE> : Sets the syringe valve in needle position 0 0

<WASTE> : Sets the syringe valve in waste position 1 0

22

3.5 INJECTOR VALVE SERVICE MODE

INJECTOR VALVE

SENSORS LOAD:X INJECT:X

<LOAD> <INJECT>

SENSORS

LOAD: X : Indicates the status of the load position sensor

This sensor is used to detect the position of the

injector valve

= 0 Sensor vane is outside the load sensor

= 1 Sensor vane is inside the load sensor, valve in LOAD

position.

INJECT: X : Indicates the status of the inject position sensor

This sensor is used to detect the position of the

injector valve

= 0 Sensor vane is outside the inject sensor

= 1 Sensor vane is inside the inject sensor; valve is in

the INJECT position.

SOFTKEYS

<LOAD> : Switches the Injector valve to the load position

<INJECT> : Switches the Injector valve to the inject position

23

3.6 EXTERNAL I/O

SERVICE MODE [MENU]

EXTERNAL I/O: INPUTS

STOP INP=X FREEZE=X NEXT INJ=X

STOP INP= X : Indicates the status of the stop input

FREEZE= X : Indicates the status of the freeze input

NEXT INJ=X : Indicates the status of the next injection input

IF X = 0 The input is low

IF X = 1 The input is high (active)

SERVICE MODE [MENU]

EXTERNAL I/O: AUXILIARIES AUX:--

<AUX1 ON> <AUX1 OFF> <AUX2 ON> <AUX2 OFF>

AUX:-- : Indicates the status of the auxiliaries

-- : Aux. 1 OFF; Aux. 2 OFF

1- : Aux. 1 ON; Aux. 2 OFF

-2 : Aux. 1 OFF; Aux. 2 ON

12 : Aux. 1 ON; Aux. 2 ON

SOFTKEYS

<AUX1 ON> : Switches the Aux. 1 ON

<AUX1 OFF> : Switches the Aux. 1 OFF

<AUX2 ON> : Switches the Aux. 2 ON

<AUX2 OFF> : Switches the Aux. 2 OFF

SERVICE MODE [MENU]

EXTERNAL I/O: OUTPUTS

<INJ MARK> <ALARM ON> <ALARM OFF>

SOFTKEYS

<INJ MARK> : Switches the inject marker pulse ON

<ALARM ON> : Switches the alarm output ON

<ALARM OFF> : Switches the alarm output OFF

NOTE:

The duration of the inject marker output depends on the

programmed duration in the SYSTEM SETTINGS

24

3.7 SERIAL PORT

SERVICE MODE SERIAL PORT

PROTOCOL: SPARKLINK RS232 TEST: 000

9600 BAUD, 8 DATA, NO PARITY, 1 STOP BIT

<START>

This screen provides only information concerning the serial port such as baud rate,

number of data bits, parity bit and stop bit.

No changes to these settings can be made.

SOFTKEY

It is also possible to check the communication port. Create a connection between pin 2

and 3 on the RS 232 connector.

After this connection is made, press the softkey <START>

If the Communication port is correct, OK will be displayed.

NOTE:

If the communication test is OK but no communication with

other devices is possible, check for a correct setting of the

DEVICE IDENTIFIER.

(System Settings [MENU] <SERIAL> Device Identifier 60-69)

The Spark-link protocol uses the device Identifier 60

25

3.8 PIERCE TEST

The pierce test is a quick and convenient way to check the piercing precision of the

needle in the vials.

In case of a 96 vial tray or an 84+3 vial tray the following sequence will carried out:

The needle arm always starts with vial number 01 and moves to 4&5, 8&9, 12&13,

…..etc.

SERVICE MODE NEEDLE PIERCING POINT TEST

VIAL NUMBER:XX ERRORS:XXX

TRAY:XXX NEEDLE:XXX

The needle piercing point test starts direct after selecting the softkey <PIERCE>

To stop the piercing point test, press the “Start/Stop” button.

VIAL NUMBER : Actual vial number.

ERRORS : Total number of tray and needle errors.

TRAY : Number of tray errors

NEEDLE : Number of needle errors

CAUTION

CAUTION:

The instrument starts the pierce test immediately after

pressing the softkey <PIERCE>

26

3.9 LIFE TEST

With the Life test it is possible to select all or some softkeys to test the functions of the

selected items for a longer period.

The life test will start after selecting the items and pressing the “Start/Stop” button.

For stopping the life test, press the “Start/Stop” button again.

SERVICE MODE LIFE TEST [MENU]

SELECTION:

<TRAY> <NDL-HOR> <NDL-VER> <INJ-VLV>

SERVICE MODE LIFE TEST [MENU]

SELECTION:

<SYRINGE> <SYR-VLV>

SOFTKEYS <TRAY> : Softkey to test the tray drive

<NDL-HOR> : Softkey to test the horizontal needle movement

<NDL-VER> : Softkey to test the vertical needle movement

<INJ-VLV> : Softkey to test the injection valve

<SYRINGE> : Softkey to test the syringe

<SYR-VLV> : Softkey to test the syringe valve

SERVICE MODE LIFE TEST

cycles:0000010 errors:002

TRAY:000 NEEDLE:001 VALVE:001 SYR:000

Example of life test screen with needle and valve unit errors.

cycles : Total number of cycles of the executed life test

errors : Total number of errors of all the programmed devices

TRAY : Number of tray errors

NEEDLE : Number of needle errors

VALVE : Number of valve errors

SYR : Number of syringe errors

ATTENTION:

The software is also counting the number of cycles and the

number of errors during the life test.

27

3.10 RESET LOG COUNTERS

RESET LOG COUNTERS

SELECT LOG COUNTER TO RESET:

<INJ VLV> <SYR-VLV> <SYRINGE>

This function in the service mode is used to clear the LOG COUNTERS. These LOG

COUNTERS are in the normal operation mode read-only.

It is only possible to reset these counters in the Service mode and not in the normal

operation mode.

SOFTKEYS

<INJ VLV> : Resets the counter of the injection valve to zero

<SYR-VLV> : Resets the counter of the syringe valve to zero

<SYRINGE> : Resets the counter of the syringe.

CAUTION

CAUTION:

Reset ONLY a counter when the corresponding part is

replaced.

28

4 DISASSEMBLING

The Midas Autosampler is build up around the mainframe, which is a part of the bottom

plate.

On this mainframe are the different assemblies placed, such as injection valve assembly,

syringe assembly.

This paragraph describes how the different assemblies can be removed, repaired or

replaced.

The adjustments for the assemblies are given in chapter 5.

Tricks and Installation tips are given throughout this whole chapter.

The first section describes how to remove the top cover, this to view the mechanical

parts.

WARNING

WARNING:

Disconnect power before removing covers.

29

4.1 REMOVING THE TOP COVER To get access to the most subassemblies it is necessary to remove the top cover,

therefore proceed as follows:

• Open the Keyboard Display cover

• Remove the Oven cover

• Remove the 4 screws marked F on the front panel

F

F

F

F

• Close the Keyboard/Display cover

• Remove the 6 marked screws R on the Rear side.

R R

R R

R R

• Lift up the Top cover to remove it.

30

4.2 INJECTION VALVE

The Midas can be equipped with the following type of injection valves:

• Valco C2-2006 valve with quick connect mounting

• Valco C2-2346 PEEK valve with quick connect mounting

• Rheodyne 7739 valve with quick connect mounting

• Rheodyne biocompatible 9740 valve with quick connect mounting.

The next sections describe how to exchange the valve and the valve drive assembly.

4.2.1 Replacing the Injection Valve

To remove the valve proceed as follows:

• Disconnect all tubing from the valve.

Only the loop can stay in place.

• Place the needle arm in the front position,

From the Ready menu press:

<MAINTENANCE> <NDL HRZ>.

• Push the levers “A” backwards to release

the valve.

• Pull the valve out of the instrument while

turning the valve counter-clockwise.

To re-insert the valve proceed as follows:

• Hold the valve for the mounting with port 1

position facing up.

• Push the valve into the mounting and turn

the valve clockwise till it clicks in the

fitting.

• Re-connect all tubing to the valve.

• Make sure that the valve is in the INJECT

position. This can be done in the

Maintenance menu.

• Press <MAINTENANCE> <VLV LOAD>

<VLV INJECT>

A

A

31

4.2.2 Replacing the Valve drive assembly

For replacing the valve drive assembly, proceed as follows:

• Remove the injection valve (See section 4.2.1)

• Loosen the 2 screws marked V

• Open the rear panel (See section 4.1)

• Disconnect the valve drive assembly connector J9 of the control board

• Remove the entire valve drive assembly backwards out of the instrument.

See for more detailed information of the valve drive assembly drawing 71.0830.301

32

4.2.3 Maintenance for Valco C2-2006/2346 valve

Cleaning a valve can often be accomplished by flushing all lines with appropriate

solvents.

NOTE:

Do not disassemble the valve unless system malfunction is

definitely isolated to the valve.

Disassembly:

• Use a 9/16 hex driver to remove the socket head screws which secure the cap on the

valve.

• To insure that the sealing surface of the cap is not damaged, rest it on the outer face.

Or, if the tubing is still connected, leave it suspended by the tubing.

• With your fingers or small tool, gently pry the rotor away from the driver.

• Examine the rotor sealing

surface for scratches. If

scratches are visible to the

naked eye, the rotor must be

replaced. If no scratches are

visible, clean all the parts

thoroughly with an appropriate

solvent, taking care that no

surfaces get scratched. (The

most common problem in HPLC

is the formation of buffer

crystals, which are usually

water-soluble) It is not

necessary to dry the rotor.

Reassembly:

• Replace the rotor in the driver, making sure that the rotor sealing surface with its

engraved flow passages is facing out. The pattern is asymmetrical to prevent

improper placement.

• Replace the cap. Insert the two socket head screws and tighten them gently until both

are snug. Do not over-tighten them - the screws simply hold the assembly together

and do not affect sealing force, which is automatically set as the screws close the cap

against the valve body.

• Test the valve by pressurising the system. If it doesn’t hold pressure, the valve should

be returned to Valco for repair.

Stator

screws

Stator

Rotor

seal

Drivershaft

Valve

body

33

4.2.4 Maintenance on the Rheodyne Model 7739 valve

With normal use the valve will give many tens of thousands of cycles without trouble.

The main cause of early failure, which is seen as a leak in the valve, is abrasive particles

in the sample and/or mobile phase which can scratch the rotor seal.

Following is the procedure for changing the rotor seal:

• Remove the three stator screws with the 9/16” hex key.

• Remove the stator and stator ring from the valve body.

• Pull the rotor seal off the pins.

• Install the new rotor seal. The three pins

on the shaft assembly fit into the mating

holes in the rotor seal only one way.

Mount the seal with the grooves facing the

stator.

• Replace the stator ring so that the body

locating pin in the stator ring enters the

mating hole in the body.

• Mount the stator on the valve so that the

stator locating pin in the stator ring enters

the mating hole in the stator.

• Replace the three stator screws. Tighten

each an equal amount until the screws are

tight.

Shaft Assembly

Body

Rotor Pins

Isolation Seal

Rotor Seal

Stator Ring

Stator

Location Pin

Stator

Stator Screws

Exploded view of Model 7739.

34

4.2.5 Maintenance on the Rheodyne Model 9740 valve

CAUTION

Use only plastic ferrules in the stator ports. Metal ferrules

can cause irreparable damage to the plastic stator.

With normal use the valve will give many tens of thousands of cycles without trouble.

The main cause of early failure, which is seen as a leak in the valve, is abrasive particles

in the sample and/or mobile phase which can scratch the rotor seal.

Following is the procedure for changing the rotor seal, stator face assembly.

• Remove the three stator screws with a 9/64 inch hex key.

• Remove the stator, stator face assembly and stator ring from the valve body.

• Pull the rotor seal off the pins.

• Mount the new rotor seal. The three pins on the

shaft assembly fit into the mating holes in the

rotor seal only one way. Mount the seal with

the grooves facing the stator.

• Replace the stator ring so that the body

locating pin in the stator ring enters the mating

hole in the body.

• Put the new stator face assembly on the stator.

The three pins on the assembly fit the mating

holes in the stator only one way.

• Mount the stator and stator face assembly on

the valve so the stator locating pin in the stator

ring enters the mating hole in the stator.

• Replace the three stator screws. Tighten each

an equal amount until the screws are tight.

Body

Shaft Assembly

Isolation Seal

Rotor Seal

Body Location Pin

Stator Ring

Stator Location Pin

Stator Face

Assembly

Stator

Stator Support

Ring

Stator Screws (3)

Exploded view of Model 9740.

35

4.3 DISPENSER

4.3.1 Removing the dispenser

For removing of the dispenser proceed as follows:

• Remove the top cover (See section 4.1)

• Remove the waste, wash and buffertubing.

• Remove the flatcable of the Keyboard/Display

• Remove the dispenser connectors J2, J3, J4, J5, J6 and J7 from the Control PCB

• Remove the connector of the Needle down sensor which is located near the needle

arm (See drawing)

D

D

D

D

• Remove the 4 marked screws D

Move the top of the dispenser downwards, until the entire dispenser is horizontal, and lift

the dispenser up.

For checking the correct function of the dispenser, re-connect the Connectors J2, J3, J4,

J5, J6 and J7

For more detailed information see section 3.4 (Service Mode: SYRINGE)

36

4.3.2 Dispenser valve

For replacing the dispenser valve proceed as follows:

• Remove the dispenser assembly out of the instrument (See section 4.3)

• Turn the syringe spindle counter clock wise, and remove the syringe.

• Remove the 2 screws from the top cover of the dispenser

• Remove the top cover of the dispenser by lifting it up

• Release belt tension by loosening the stepper motor mounting screws 1 turn and

sliding the motor to the valve side.

• Remove the sensor disc

• Loosen 1.5mm hex screw from the valve pulley and lift the valve pulley up

• Loosen the valve nut and remove the valve downwards

Replace the syringe valve and re-assembly the entire dispenser.

Since the sensor disk takes care for the correct position of the valve, there is no initial

position for replacing the valve.

NOTE:

After replacing the syringe valve the LOG COUNTERS for the

syringe valve should be RESET.

From Ready Mode: [MENU] <SERVICE> 010466 [MENU]

[MENU] <RESET LOG> <SYR VLV>

37

5 ADJUSTMENTS

5.1 NEEDLE HEIGHT ADJUSTMENT

To adjust the needle height of the autosampler proceed as follows:

• Power up the autosampler in the Service Mode.

• Place an uncapped vial with the highest possible bottom in the tray.

Needle height

adjustment screw

Needle body

• Loosen the needle height adjustment screw.

• Set the Needle body as high as possible.

• Tighten the adjustment screw.

• Move the needle arm downwards using the Service Mode (See section 3.2)

• Loosen the needle height adjustment screw

• Move the needle body manually downwards until the tip of the needle is approximately

1 mm above the bottom of the vial.

• Tighten the needle height adjustment screw

CAUTION

CAUTION:

Make sure the vial type used for adjustment of the needle height, is the

vial type with the highest bottom. See drawing above for examples of vial

types with different bottom heights.

38

5.2 SYRINGE HOME ADJUSTMENT

To adjust the home position of the syringe, proceed as follows:

• Check if the syringe is installed correctly. Completely tightened in the luerlock, or for

syringes without a luerlock, complete tightened in the syringe valve.

• Go to Service Mode concerning Syringe (See section 3.4)

• Loosen the 2 screws that secure the home vane sensor.

• Slide the home vane upwards as far as possible.

• Press the Softkey “HOME”.

• Slide the sensor vane downwards, and

press the Softkey “HOME”.

• Repeat these two actions until the

distance between the tip of the plunger

and the end of the syringe is

approximately 2 mm.

• Tighten the two screws of the sensor

vane.

±2mm

39

5.3 PENETRATION POINT ADJUSTMENT

The penetration point of the Midas can be adjusted in two different positions. The needle

should be centred in the vial and therefore it is adjustable into the left-right position as

well as into the front- back position.

Examples of wrong penetration points:

Needs: Left-Right

Adjustment

Needs: Front-Back

Adjustment

Needs: Front-Back and

Left -Right

Adjustment

Needs: Left-Right and

Front-Back

Adjustment

5.3.1 Left Right adjustment

For left right adjustment of the penetration point into the vial proceed as follows:

• Remove the sample needle.

• Remove the Top Cover (See section 4.1)

• Go to Service Mode (See section 3.2)

• Set the needle arm above the wash position. (NEEDLE HORIZONTAL <HOME>)

• Move the needle arm downwards (NEEDLE VERTICAL <DOWN>)

• Loosen the 2 marked screws P ½ turn.

(Use a screwdriver with a long shaft for easy access to the screws)

• Rotate the entire needle unit in such a way that the air needle is centred in the wash

position.

(Use the stepper motor as a grip for rotating the needle unit)

• Fasten the 2 screws marked P

• Check the centre position on several vials and on the wash position.

• Re-install the sample needle.

40

5.3.2 Front Back adjustment

For changing the stop position of the tray, proceed as follows:

• Remove the entire tray disk

• Loosen the 3 screws marked Q. ½ Turn.

• Turning the Tray stop adjustment screw clockwise will result in an earlier stop


• Turning the Tray stop adjustment screw counter clockwise will result in a later stop


• Fasten the 3 screws marked Q

• Replace the tray disk and check the penetration point on several positions.

NOTE:

For quick and easy testing of the penetration point, use the

Piercing mode of the Service Mode

(See section 3.8)

41

5.4 VIAL STRIPPER ADJUSTMENT

The vial stripper of the Midas protects the vials for lifting up out of the tray, during the

needle up movement of the needle arm.

For installing or removing the tray-disk or the cooling cover, it is necessary to push the

vial stripper into the direction of the Injection valve. The spring behind the vial stripper

takes care for resetting the stripper to the initial position.

If the penetration has been re-adjusted in the left-right direction it is sometimes

necessary to re-adjust also the vial stripper.

The vial stripper can be adjusted by turning the marked setscrew

42

5.5 VIAL SENSOR ADJUSTMENT

The Midas is standard equipped with a vial sensor. This vial sensor checks for sample

vials in the tray during the needle down movement of the needle arm.

The vial sensor also controls the vertical stop position of the needle arm.

In case of a programmed 24 Large sample vial tray or using 2.5 ml vials, the needle arm

stops direct when the air needle has punctured the septa.

The air needle will be in this situation not in contact with the sample in the vial, but after

aspirating 50% of the total vial volume, the needle arm moves further downwards.

This sequence is to avoid sample between the air needle and the liquid needle.

The distance between the vial

sensor and the top of the tray

should be in the down position of

the needle arm approximately 1.5

mm.

• This can be adjusted by

loosening the two small

screws on the top of the vials

sensor indicated V.

• Re-adjust the height and

tighten the screws.

For vial sensor replacement, proceed as follows:

• Remove the top cover (See section 4.1)

• Remove the needle and needle tubing from the needle arm.

• Move the needle arm down (use the Service Mode)

• Loosen the hex screw

marked S

• Lift up the entire needle

arm, and slight it

backwards out of the unit.

• Remove the marked

screws T, and replace the

entire vial sensor assembly

including wiring and air

supply tubing.

1.5mm

V

V

T

T

T

Vial Sensor S

43

6 FIRMWARE REPLACEMENT

The firmware of the Midas is placed in an EPROM which is located on the CPU Board.

ATTENTION:

The EPROM is highly sensitive for Electro Static

Discharging.

Observe precautions for handling Electrostatic discharge

sensitive devices.

For replacement of this EPROM proceed as follows:

• Make a notice of all the programmed

Methods, Series and System Settings

• Turn mains power off

• Remove the 4 marked screws A

• Open the rear panel

It is now possible to remove the old

EPROM and place the new EPROM

Make sure all pins of the EPROM are

placed in the IC-socket.

• Check all settings after placing a

new EPROM

EPROM

NOTE:

The little notch on the top of the EPROM should be facing the

same way as the notch on the IC-socket.

A A

A A

44

7 TROUBLE SHOOTING

7.1 ERROR CODES

The Midas is equipped with a sophisticated fault system. Every malfunction will be

reported as an Error code.

In the list below, each error code is described and also the action to solve the mentioned

error is given.

Complete this list yourself during your experience with the Midas and use it as a help

guide for quicker solutions and decrease the time to locate the fault.

7.1.1 INJECTION VALVE UNIT

ERROR CAUSE ACTION

211 Injection valve is not in a valid

position. Output of both sensors is high (1)

• Check the output of the optical

sensors in the Service Mode.

• ………………………………………..

212 The injection valve did not

switch within 1.5 seconds.

• Check for any loose parts

• Check the stepper motor

• Check belt tension

• Check the optical sensors

• …………………………………………

213 The switching time of the

injection valve exceeds 500

msec

• Check rotor seal and stator (torque to

turn the valve might be increased

dramatically)

• …………………………………………

45

7.1.2 SYRINGE DISPENSER UNIT

ERROR CAUSE ACTION

221 The syringe valve did not switch.

(Status of the activated

sensor(s) is not changed)

• Check the two sensors in the Service

Mode (turn valve by hand using the valve sensor disc as a grip)

• Check the stepper motor

• Check the belt tension


• …………………………………………

222 The syringe did not reach the

home position within time.

(Spindle rotates correctly)

• Check screw thread in transport block

assy, if worn out replace transport block

• …………………………………………

223 The syringe spindle did not make

the correct number of rotations.

(The number of pulses from the

rotation sensor does not

correspond with the number of steps send to the stepper motor)

• Remove syringe and retry without

• If OK check flow path (buffertubing

inj. valve and needle for obstructions or over tightened ferrule)

• If error 223 still occurs without

syringe check:

• Optical rotation sensor

• Stepper motor

• Belt tension

• Spindle for too high torque

• Broken pulley

• …………………………………………

224 The spindle does not rotate.

(No rotation pulse found)

• Remove syringe and retry without

• If OK check flow path (buffertubing

inj. valve and needle for obstructions

or over tightened ferrule)

• If error 224 still occurs without syringe check:

• Optical rotation sensor

• Stepper motor

• Belt tension

• Spindle for too high torque

• Broken pulley

• …………………………………………

225 The syringe valve did not find a

valid position.

(Motor is turning but no stop position is found)

• Check sensors in Service Mode

• Check for any loose parts; pulley, belt

tension

• …………………………………………

46

Checkpoints for obstructions in flow path of the Midas Autosampler

To locate an obstruction in the flow path, perform a wash-routine and check carefully the

flow path.

For more detailed information see section 7.2

Syringe valve

Buffer tubing

Injection valve

Sample needle

47

7.1.3 NEEDLE UNIT

ERROR CAUSE ACTION

230 The sample needle arm did not

reach or leave the home position

(vertical).

(Status of sensors does not change)

• Check home sensor (on PCB)

• Check movement in Service Mode of Linear stepper motor

• Check for any mechanical obstructions

(bent needle, tubing behind loop …)

• …………………………………………

231 The sample needle arm is in an

invalid horizontal position while moving down.


• Check bearing of the needle arm

• Check for a bent guiding shaft

• …………………………………………

232 The sample needle arm did not

reach its destination within a

certain time (horizontal).

• Check Needle left-right sensor

• Check for mechanical obstructions

• Check for any loose part

• …………………………………………

233 Too many or not enough steps

needed to reach destination of horizontal needle movement.

• Check for any loose part

• Check stepper motor horizontal drive

• …………………………………………

234 Sample needle arm not in

vertical home position while moving horizontally.

• Check in Service Mode if light-beam of

vertical home sensor is changing during a horizontal movement

• …………………………………………

235 The sample needle arm is at an

invalid horizontal position

• See error 233

• …………………………………………

237 The sample tray is not at a valid

position while moving the sample needle arm down.

• Check if sample tray is correct placed

on the tray axle

• Check needles on bending

(Tray can be moved by a bent needle)

• …………………………………………

239 Vial sensor sticks.

(Status of the vial sensor is still 1)

• Check vial sensor in Service Mode

(1=light-beam interrupted)

• Check for any spilled liquid on sensor, axles or springs

• …………………………………………

48

Top view of the Needle unit without the needle sensor PCB.

Needle Left-

Right sensor

Needle Down

sensor

Vial sensor

The Needle sensor PCB with the Needle Wash Sensor and the Needle Up Sensor.

Needle Wash

Sensor

Needle Up

Sensor

49

7.1.4 TRAY UNIT

ERROR CAUSE ACTION

251 Incorrect tray rotation

(During tray rotation, status of

the stop sensor remains the same)

• Check tray stop sensor in Service

Mode

• Check correct placing of the sample tray disk on the tray axle

• …………………………………………

252 No sample tray positioned in the

Midas.

• Check correct placing of the sample

tray disk on the tray axle

• Check stop sensor and tray home

sensor

• …………………………………………

253 The sample needle arm is not in

the home position while rotating

the tray.

• Check status of the vertical home

sensor needle arm in Service Mode

while rotating the tray manually

• ………………………………………….

50

7.1.5 VIALS

ERROR CAUSE ACTION

260 Missing vial.

(Only available when Skip

Missing Vial is set to NO in the

System Settings

• Check if programmed range of vials is

placed in tray disk

• Check vial sensor

• ……………………………………….

262 Missing transport vial.

(Only available when Skip

Missing Vial is set to No in the

System Settings

• Check if transport vials are placed in tray disk


• ……………………………………….

264 Missing vial for reagent A • Check if reagent vial A is placed in tray disk


• ……………………………………….

265 Missing vial for reagent B • Check if reagent B is placed in tray

disk


• ………………………………………..

268 Missing destination vial. • Check if programmed range of

destination vials are placed in tray

disk


• ………………………………………..

269 Not enough transport liquid

available due to missing transport vials.

Check if programmed range of vials

using µl pick-up method not exceeds

maximum vial volume of the transport

vial(s) See for calculation the Users

Manual

270 Not enough reagent A available. Check if programmed volume not

exceeds the volume of the reagent A vial.

See for calculation the Users Manual

271 Not enough reagent B available. Refer to Error 270

7.1.6 ELECTRONICS

ERROR CAUSE ACTION

275 Error occurred during

initialisation, the Midas can not start.

• An essential part is not properly

working and the Midas can not execute the programmed method

Turn mains power off, power up the unit

again and check for other error codes for

more details

51

7.2 ANALYTICAL

In cases of analytical problems the best thing to start with is to determine, if the cause

for the problem is in the Autosampler, or in the rest of the system.

In order to do this, replace the Autosampler by a manual injector and do some manual

Full loop injections.

If the results are fine the fault has to be found in the Autosampler, if not the HPLC

system should be checked.

Syringe Valve

Syringe

Buffer Tubing

Injection Valve

Needle arm

Air Needle

Injection Needle

NOTE:

In the diagrams on the next pages it is assumed that the unit

is working without Errors.

Please keep in mind that analytical problems also might be

caused by external influences, like temperature and or light

sensitive samples. For this reason it is important to be sure the

application was running without problems before and nothing

has been changed.

52

Start

manual

wash

Solvent flowing

out of inj. portCheck needle

Disconnect

Buffer tubing

from valve

Start

manual

wash

Solvent out of

open end

Check rotor

seal

Blockage in

Flow Path

Disconnect

needle from

Valve

NO

INJECTION

Disconnect

Buffer tubing

from Syringe

valve

Start

manual

wash

Solvent out of

syringe valve

Check

buffer

tubing

Check for over tightened

connections in the entire

Flow Path &

Check the syringe valve

Leakage inside

Injection Valve

Disconnect the needle tubing

and the buffer tubing.

Connect port 1 to an HPLC pump

Block port 6.

Start the pump at a low flow

Observe ports

3 and 4 for leakage

Check rotor

seal

Recheck with manual valve

Yes

Yes

No

Yes

Yes

No

No

No

53

BAD

REPRODUCIBILITY

Air in Flow Path Perform a manual wash

Leaking Syringe

Leaking Syringe Valve

Rotor seal worn out

Dead volumes in

tubing connections

Replace plunger tip or Syringe

Replace Valve

Replace seal.

Check Stator

Redo connections with new

Ferrules and nuts

Air in Syringe Remove Syringe and fill

manually with wash solvent

54

8 NEEDLE

The Midas is standard equipped with a stainless steel needle pair.

The internal diameter of injection needle as well as the tefzel tubing from needle to valve

is 0.25 mm.

The injection needle is only available as a complete subassembly including nuts and

ferrules.

The nuts and ferrules are hand tight fittings and fits into the Rheodyne valve and the

Valco valve.

The air needle is supplied including a seal to avoid leaking of the air supply.

For samples which will react or stick to stainless steel, a coated needle is available on

which the inner side as the outer side is coated

For more viscose samples a large bore sample needle is available with an ID of 0.5mm.

Install also a large bore air needle when using a large bore sample needle.

8.1 NEEDLE CONNECTIONS

Number Description Part number

1 Finger tight ferrule PEEK

2 PEEK ferrule

3 Tefzel tubing L=150 mm (drilled up)

4 Nut

5 Teflon ferrule

6 Conical ring

7 Stainless Steel Sample Needle 830

8 Part of Silicon tubing

Needle assy: 0830.303

9 Needle body 0042.793

10 Air needle 830 (incl. Seal) 0830.718

11 Vial feeler assy 0830.765

12 Hex screw (for needle height adjustment) 6450.030

13 Tygon tubing 7100.682

55

9 PELTIER COOLING OPTION

Thermo-electric sample cooling is available on the Midas as a fully integrated option.

No external device is required and the efficient cooling concept does not restrict access to

the sample tray during operation.

The sample vials are temperature controlled by using two Peltier elements.

The Peltier elements are mounted between a cooling ring and two heat sinks.

Since the Peltier elements are working with a cooled side and heated side, the two fans

are used for a forced air cooling of the heat sinks

The programmable range of the cooling option is from 4°C till 15°C, and the cooling

capacity is 20°C below T ambient with a max. of 60% relative humidity and a Maximum

ambient temperature of 30°C

See for spare part numbers and more detailed information drawing 71.0830.840 in this

Manual.

56

9.1 PELTIER ELEMENT REPLACEMENT

In case of malfunction of the Cooling option proceed as follows:

• Turn OFF the mains power of the Midas.

• Open the rear-panel and remove the connector J1

• Connect a resistor meter to Connector J1. The value should be below 100 Ω

If NOT: One of the Peltier elements is defective. (It is advisable to replace both

Peltier elements if one of them is defective.)

• Remove the tray disk.

• Mark before removing the stop

sensor disk, the position of the disk

with respect to the bottom frame

with a pencil

• Remove the 4 screws of the cooling

ring two by two.

• Remove the temp. sensor

assembly from the cooling ring.

• Lift up the cooling ring carefully.

• Cut the wires of the defective

Peltier elements

• Remove the bottom plate of the

Midas.

• Pull out the heat sinks and the

Peltier elements

• Install the new Peltier elements

including the wiring and the heat

sinks. Add some new thermal joint

compound (Apply thin !!) on both

sides of the Peltier elements.

• Re-install the bottom plate.

• Solder the wires of the Peltier

elements to the existing wires and

isolate them with shrink tubing.

• Re-install the cooling ring and

connecting plates and tighten the

screws two by two.

• Re-install the temp. sensor

assembly, the stop sensor plate

and the tray-disk.

• Re-connect connector J1 and close

the rear-panel.

• Turn ON the mains power from the

Midas and check the cooling option for proper functioning.

CAUTION

CAUTION:

Peltier elements are fragile; therefore take care during

replacing the elements.

• Do not bend the two solder connections of the Peltier

elements.

• Do not over-tighten the screws of the cooling ring and

tighten them two by two.

57

10 LIST OF ACCESSORIES

Part Number Description

0042.565 Male nut M6

0042.793 Needle holder

0830.811 Inject marker cable

0830.711 Serum air needle (for use in combination with serum sample needle)

0788.711 PREP air needle (for use in PREP option only)

0830.718 Standard air needle, including seal

0830.303 Sample needle MIDAS (including tubing nuts & ferrules)

0830.304 Serum sample needle MIDAS (including tubing nuts & ferrules)

0830.318 BIO-Inert Silc sample needle MIDAS (including tubing nuts & ferrules)

0830.835 Large Sample Volume kit MIDAS (incl. serum needles, 500 µl loop 1000

µl syringe, 2000 µl buffertubing and 24 position 10 ml tray-disk)

0830.836 PREP option field installable (incl. prep air needle, serum sample

needle, 10.000 µl loop, 2500 µl syringe 2000 µl buffertubing, PREP

injection valve and 24 position 10ml tray-disk)

0830.316 Large Capacity option 96 vial positions

0900.710 Buffer tubing 500 µl

0900.711 Buffer tubing 2000 µl

0900.712 Syringe waste tubing

0830.718 Syringe wash tubing

2140.160 Luer lock connector syringe valve

0830.731 7739 Rheodyne inj. Valve Stainless Steel version incl. bayonet pins.

0830.732 9740 Rheodyne inj. Valve PEEK version incl. bayonet pins

0830.733 Valco injection valve Stainless Steel C2-2006 SPHMI incl. bayonet pins

0830.734 Valco injection valve PEEK C2-2346 SPHMI incl. bayonet pins

0830.736 Valco PREP injection valve C2-3006 SPHMI incl. bayonet pins

3796.083 Valco fingertight nut PEEK

3796.084 Valco ferrule PEEK

3796.046 Valco 5 µl Loop Stainless Steel

3796.029 Valco 20 µl loop Stainless Steel




3796.094 Valco 10.000 µl loop Stainless Steel

3796.088 Valco 20µl loop PEEK

3796.085 Valco 100 µl loop PEEK

3796.043 Valco rotor seal for C2-2006

3796.044 Valco Stator for C2-2006

3796.048 Valco PEEK rotor seal for C2-2346

3796.087 Valco PEEK Stator for C2-2346

3796.110 Valco rotor seal for C2-3006

3796.111 Valco Stator for C2-3006

3796.091 Rheodyne stator for 7739 injection valve

3796.092 Rheodyne VESPEL rotor seal

3796.093 Rheodyne TEFZEL rotor seal

3796.095 Rheodyne PEEK stator for 9740 injection valve

3796.096 Rheodyne stator face assy for 9740 injection valve

3796.097 Rheodyne PEEK rotor seal

58

Part Number Description

3796.035 Rheodyne loop 5 µl Stainless Steel








3796.076 Rheodyne PEEK loop 20 µl

3796.016 Rheodyne PEEK loop 100 µl

0700.001 Teflon ferrule 1/16″ (pck of 10)

0700.008 Rheodyne Rhe-Flex ferrule (pck of 10)

0700.009 Rheodyne Rhe-Flex nut (pck of 10)

0700.010 Flanged tubing fitting 1/8″ (pck of 5)

0700.011 Flanged tubing fitting 1/16″ (pck of 5)

4340.025 Wash solvent bottle 250 ml

4400.000 Syringe 1000 µl

4400.250 Syringe 250 µl

4400.255 Syringe 2500 µl

0900.742 Plunger replacement tip 250 µl (pck of 10)

0900.750 10 ml vials (pck of 125)

0900.751 Crimp cap for 10 ml vials (pck of 125)

0900.752 Septa for 10 ml vials (pck of 125)

3760.411 cap crimper for 20 mm caps (10 ml vials)

3760.403 cap crimper for 11 mm caps (1.8 ml vials)

0700.019 Plunger replacement kit 1000 µl (pck of 10)

0830.728 Bottle holder for wash and waste bottles of 250ml

0830.790 Shipping box assembly

59

11 TEST PROCEDURE

The Midas is factory tested for reproducibility, carry over and mixing according the

following test procedure.

Analytical parameters:

Pump: Flow:

Eluent:

1.5 ml/min

distilled water

Detector: Wave

length:

254 nm

Sample: Uracil in distilled water (50 ppm)

Relative standard deviation (RSD%)

The formula’s necessary for calculating the Relative Standard Deviation (RSD%) are

printed below:

( )

%100%

1

1

2

1

×=

−

−=

=

−

−

∑

∑

areaPeakRSD

n

areaPeakareaPeak

n

areaPeakareaPeak

n

n

σ

σ

60

Reproducibility and carry over test.

Seven vials are placed in the Midas tray, vial 1 and vials 3 till 7 are filled with sample

(Uracil 50 ppm) and vial 2 is filled with eluent.

The wash solvent bottle is filled with 80% H2O / 20% Iso-propanol

The vials are run with the following method:

Injection

Injection mode: Partial loopfill

Flush volume: 30 µl

No of inj./vial: 3

Injection volume: 10 µl

Analysis time: 0:01:00

Wash

Wash between: None

An example chromatogram of the reproducibility test is printed below.

From the integration results the Relative Standard

Deviation (RSD%) and the carry over can be calculated.

For this example the results are:

RSD%= 0.25%

Carry over < 0.5%

Peak

number

Area

1 206342

2 204773

3 205425

4 -

5 -

6 -

7 205203

8 205566

9 205568

10 206156

11 206174

12 205956

13 206165

14 205765

14 205960

16 206296

17 205617

18 205619

19 205648

20 206141

21 207070

61

Mix test:

For the mix test one sample vial (50 ppm Uracil), one empty vial and the Reagent-A vial,

filled with eluent, are placed in the Midas tray. A 1:10 dilution of the sample will be made

in the empty destination vial. After the dilution from both vials (sample and destination)

10 µl will be injected.

The wash solvent bottle is filled with 80% H2O / 20 % Iso-propanol.

The vials are run with the following methods:

Method 1:

Injection



No of inj./vial: 3



Wash

Wash between: None

Mix

1. ADD 180 µl REAG-A to DESTINATION

2. ADD 180 µl REAG-A to DESTINATION

3. ADD 40 µl SAMPLE to DESTINATION

4. MIX 3 times with 250 µl

5. END OF MIX METHOD

Method 2:

Injection



No of inj./vial: 3



Wash

Wash between: None

Program the following Series:

Series 1 Series 2

Method: 1 Method: 2

First vial: 01 First vial: 01

Last vial: 01 Last vial: 01

Destination vial: 02

62

An example chromatogram of the mixing test is printed below.

0.00

0.02

0.04

0.06

0.08

0.10

0.12

0 1 2 3 4 5 6 7 8 9 10

Time

From the integration results the Relative Standard Deviation (RSD%) for the two

vials can be calculated.

For this example the results are:

RSD% (vial 1, 1:10 dilution) = 0.37%

RSD% (vial 2, sample) = 0.15%

1:10 dilution = 1 : 10.13

Peak

number

Area

1 38802

2 38968

3 39088

4 395258

5 394090

6 394471

63

Exploded view drawings and schematics

64

0830.302 Valve drive assembly parts list

0830.302 Valve drive assembly

Component Description Quantity

0043.257 Sleeve bearing 1

0043.258 Bearing axle 1

0043.559 Sensor motorplate 1

0043.560 Valve plate 1

0043.561 Connecting axle 1

0043.562 Valve lever 1

0043.563 Sensor flag 1

0043.564 Column 0830 1

0043.565 Locking plate 1

0043.566 Valve spring 2

0043.567 Motor lever 1

0043.701 Supportblock 830 1

0043.717 Bayonet spring 1

0830.805 Valve wiring set 1

2099.041 Nylon bearing 5mm 1

2291.244 Distance bush M4x7x35 2

2562.349 Optical sensor TLP 1201A 2

0830.737 Repl. steppermotor 1.05A incl valve wiring 1

6405.051 Bolt M5x10 SS 1

65

Exploded view of Valve drive assembly

66

0900.302 Syringe Dispenser assembly parts list 0900.302 Syringe dispenser assembly


0043.243 Valve sensor disc 1

0043.244 Guiding block top 1

0043.245 Sensor bracket 1

0043.246 Sensor protector 1

0043.247 Bottom plate dispenser 1

0043.248 U-profile dispenser 1

0043.249 Top plate dispenser 1

0043.396 Pulley 36 MXL 050 1

0043.405 Sensor disc 1

0043.789 Valve bush 1

0043.791 Plunger holder 1

0043.949 Guiding block 1

0043.950 Interuport 1

0900.763 Spindle replacement assembly 1

0045.020 Plunger screw 1

0045.022 Syringe clamp spring 1

0900.807 Wiring set dispenser 1

2033.076 Geared belt 760 MXL 025 1

2033.536 Geared belt PU 520 MXL 025 1

2034.003 Pulley PLA 18 MXL 025 1

2034.008 Pulley 18DF MXL Alu 2

2100.002 Ball bearing 2

2140.151 Syringe valve 1

2179.530 Spring 1

2562.349 Opto interruptor 2

0900.746 Steppermotor 1.05A 1


6050.030 Setscrew M3x4 1

6406.014 Philips ss countersink screw M3x6 2

6406.015 Philips ss countersink screw M3x8 2

6412.054 Hex screw M4x10 3

6416.014 Philips ss screw M3x6 2

67

Exploded view of Syringe dispenser assembly

68

0830.305 Mains inlet assy 0830 parts list

0830.305 Mains inlet assembly 0830


0043.673 Mains inlet frame 1

0051.065 Grounding label 1

0830.804 Cable bridge rectifier-mainboard 1

2524.819 Mains inlet/fuse holder/power switch 1

2563.232 Bridge rectifier 1

7500.015 Litze cable 170mm 1

9001.416 Philips screw M4x16 1

0725.620 MultiLink RS 232 interface PCB 1

0043.745 MultiLink cover plate 1

2147.154 Fan grid 1 only for cooling

2682.006 Fan 1 only for cooling

69

Exploded view of mains inlet assy 0830

70

0830.307 Needle drive assy 0830 parts list

0830.307 Needle drive assembly 0830


0043.682 Horizontal movement bush 1

0043.693 Needle drive frame 1

0043.707 Sensor plate 1

0043.722 Stripper return spring 1

0043.730 Distance bush needle arm 2

0043.731 needle sensor bracket 1

0043.732 Bearing bush needle arm 1

0043.740 Guiging bullet needle arm 1

0043.752 Sensor connecting plate 1

0830.602 Tray/Needle sensor PCB 1

0830.807 Flatcable sensor PCB-Mainboard 1

0830.812 Cable set tray/needle-needle L/R 1

0830.813 Cable set tra/needle-needle down 1

2099.041 Nylon bearing 5mm 1

2562.349 Optical interuptor 2


0830.741 Vertical needle movement motor assembly 1

71

Exploded view of needle drive assy

72

0830.308 Needle arm assy 0830 parts list

0830.308 Needle drive assembly 0830


0042.793 Needle holder 1

0043.686 Needle arm 0830 1

0830.718 Air needle including seal 1

0043.711 Vial sensor block with flag 1

0043.715 U-shaped vial sensor guiding axles 1

0043.716 Vial sensor block PEEK 1

0043.729 Needle arm sensor flag 1

0043.735 Vial sensor spring 2

0830.768 Opto assembly needle arm 1

7100.682 Tygon tubing 0.46M

2050.002 T-connector 1

6215.901 screw M1.6x4 2

6416.014 Philips screw SS M3x6 4

6450.030 Set screw SS M3x4 1

6451.002 Set screw with Nylon tap M4x10.5 1

6759.102 Cable clip 1

73

Exploded view of Needle arm assy 0830

74

0830.310 Oven assy 0830 parts list

0830.310 Oven assembly 0830


0043.017 SS oven gauze 1

0830.730 Oven bucket incl. heater 1

0810.735 Temp sensor assembly 1

0043.708 Oven door cover 1

0043.017 Oven door insulation 1

2147.149 Oven door lock 1

2291.239 Nylon distance bush 6x3x10 4

7500.010 Litze cable 1

75

Exploded view of Oven assy 0830

76

0830.320 Keyboard cover assy 0830 parts list

0830.320 Keyboard cover 0830


0043.110 shim plate for hinge 2

0043.679 Keybaord cover 0830 1

0043.684 Hinge plate 0830 1

0043.685 Protection plate 0830 1

0043.692 Flatpanel keyboard "Spark version" 1

0043.761 Foam for display protection 1

0050.084 Frontglass "Spark Midas" 1

0830.603 Keyboard PCB 1

0830.802 Flatcables keyboard/display - keyboard PCB 1

0830.815 Flatcable keyboard PCB - mainboard 1

2147.151 Hinge plate 0830 2

0830.725 Replacement display assembly 1

6759.030 Flatcable clamp 1

77

Exploded view of keyboard cover assy 0830

78

0830.401 Bottom frame assy 0830 parts list

0830.401 Bottom frame assembly 0830


0830.735 Mainframe including tray bearing 1

0043.672 Bottomplate 0830 1

0043.678 Tray motor plate 1

0043.683 Tray shaft 0830 1

0043.694 Sensor and adjustment plate 1

0043.737 Wash outlet 1

0830.332 Waste tubing assembly bottom frame 0830 1

0043.739 Guiding tube needle vertical movement 1

0043.979 Support bracket for ringcorn transformer 1

0830.808 Cable tray/needle - home position 1

2033.120 Belt 1200 MXL 025 1

0900.743 Rubber feet (pck/6) 1

2034.006 Pulley PLA 20 MXL 025 1

2034.007 Pulley PLB 120 MXL 025 1

2050.001 T-connector waste tubing 2

2179.542 Spring 1

2300.010 Rubber disc for ringcorn transformer 1

2562.349 Optical sensor 2

0900.741 Steppermotor assembly 2.2A 1

0900.744 Ringcorn transformer 1

4440.000 Funnel PP 30mm 1

79

Exploded view of Bottom frame assy 0830.401

80

Exploded view of Bottom frame assy (top view)

81

82

0830.402 Mainframe assy 0830 parts list

0830.402 Main frame assembly


0043.674 Dispenser / valve frame 1

0043.690 Vial stripper 1

0043.691 Stripper guidingblock 1

0043.696 Drip cup injection valve 1

0815.308 Membrane pump 815/830 1

0830.302 Injection valve assembly 1

0830.816 Grounding cable mainframe 1

0900.302 Syringe dispenser 1

6450.048 Setscrew SS M4x10 1

6759.072 Wire harnass WHC 375-01 2

83

Exploded view of Mainframe

84

0830.403 Rearpanel assy 0830 parts list

0830.403 Rearpanel assembly 0830


0043.671 Rearpanel 1

0051.071 Caution label 1

0830.601 Mainboard 0830 1

2147.359 Nylon washer M3 7

2524.141 Screw lock female pair 2

7500.011 Grounding cable 1

85

Exploded view Rearpanel 0830

86

0830.840 Cooling option 0830 parts list

0830.840 Cooling option 0830


0043.341 Heatsink 2

0043.345 Insulation foam bottom plate 1

0043.346 Insulation foam peltier element 1

0816.741 Temp sensor assembly cooling option 1

0043.725 Cooling cover 1

0043.726 Cooling ring 1

0043.727 screw bush heatsink 2

0043.751 Connecting plate peltier element 2

2500.042 Peltier element 2

2682.006 Fan 2

4480.006 Tape 0.2MT

6759.030 Flatcable clamp 1

6409.035 Screw SS M3x12 1

6406.132 Philips countersink screw ss M4x40 4

87

Exploded view Cooling option 0830

88

Electronic drawings and schematics

89

0830.601 CPU PCB Component view of mainboard

90

0830.601 Electronic diagram mainboard

24VDC

GND

PGND

COM2

D00

D04

COL1

U28

ULN2003

16151413121110

9

1234567

8

O1O2O3O4O5O6O7

COM

I1I2I3I4I5I6I7

GND

C99470uF/35V

U15L4960

5

1

64

7

23O

SC

INP

SSGN

D

OUT

FBFC

KEYBOARD

SYR VALVE

VCC

A09

/IACK1

DIR2

/KEYSCAN

R2822K1

D151N4004

R25

1R

/RESET

/RAMUA01

D06

D10

D13

D00

/IRQ2

A11

CLOCK1

/AS

2.4576MHz

COL4

A01

U25A

74HCT04

1 2

PS1RXE025

C106

100nF

RV3

100K/P

C101

4700uF

C71

C510.33uF

VCC

VCC

24VDC

A10

/RESET

INJ_C

dis4

VERT. MOTOR

RELAY

VCC

VCC

24VDC

GND

D10

A05

A13

D14

SCAN1

D07

U9F

7406

13 12

D03D04D05

A15

A04

COL6

AUX2_NC

C59

HEATER

VCC

GND

A04

H/F1

/RESET

AUX2_C

GND AGND

R/W

D05A02

/DTACK

PH_C

/CD2

D00 SCAN0

D14

J13

MT7/5P

12345

C92100nF

C3

L2

BL02

U9D9 8

C19

100nF

/LDS

A05

A02

D06

SENS2

C3510nF

GND

DISPLAY

24VDC

A08

A12

SCAN2

ALARM

/DISPL

D00

INJ_MARK

INJ2_NO

U26

LT1054CP

1

2

3

4 5

6

7

8FB/SD

CAP+

GND

CAP- VOUT

VREF

OSC

VCC

NEEDLE

24VDC

24VDC

GND

GND

GND

FC1

A08

/IRQ3

SW_1

AUX1_NC

RY3

RG2L 24V

468

1

16

13119

U27

AD7224KN

13121110

9876

1514

31716

4

2

18

1

D0D1D2D3D4D5D6D7

WRCS

VREFRESETLDAC

AGND

VOUT

VDD

VSS

U30

74HCT273

3478

13141718

111

256912151619

D1D2D3D4D5D6D7D8

CLKCLR

Q1Q2Q3Q4Q5Q6Q7Q8

J10

MT7/5P

12345

C100

4700uF

C22

330pF

GND

PGND

A05

/EN_INT

A10

D15

A02

NDL_DOWN

R125K1

J19MT7/3P

123

C48

C760.33uF

C30

220uF/35V

VCC

A03

/COMBO

A03D03

/RAML

D04

D08

ALARM_NC

FAN 1

D12

D06

D03COL3

D10

SCAN1

C26

6.8uF

U25E

74HCT04

11 10

PGND

+5.000V

GD

PH_B

A07

D02

D06

R/W

SENS1

R20

1K5/1W

C501nF

C86

6.8uF

D08

A17

CLK297

SENS2

D05

/FLASHDATA

SCAN0

DDS

U10

MK48Z02

87654321

232219

182021

910111314151617

A0A1A2A3A4A5A6A7A8A9A10

CEOEWE

D0D1D2D3D4D5D6D7

R1010R

INH

VSGND

U37A

L293B

1

2

3 6

7

1684 5

C28

Q1

3

2 1

FAN 2

POWER

TRAY/NEEDLE

A04

9.8304MHz

/RAMU

D07

dis4

U4E

74HCT04

11 10

C78

2.2uF

C27

+5.000V

AGND

PGND

-15VDC

A21

A11

TRAY_POS

/RESET

dis0

AUX2_NC

U32

L298

5

6

7

10

11

12

1

15

9

4

8

2

3

13

14

INP1

ENA

INP2

INP3

ENB

INP4

CSEN1

CSEN2

VSS

VS

GND

OUTP1

OUTP2

OUTP3

OUTP4

C67

VCC

COL5

HEATER

NDL_LEFT_RIGHT

SENS3

U17

TS68230CFN8

4447454643

3231302928

4849505152

5679

1112

1819222324252627

3435363738394041

4

10

123

14151617

CLKR/WCSDTACKRESET

RS1RS2RS3RS4RS5

D0D1D2D3D4

PA1PA2PA3PA4

PA6PA7

PB0PB1PB2PB3PB4PB5PB6PB7

PC0PC1

TIN/PC2TOUT/PC3

DMAREQ/PC4 PIRQ/PC5PIACK/PC6TIACK/PC7

PA0

PA5

D5D6D7

H1H2H3H4

-15VDC

D15

A15

/IRQ1/LDS

CLOCK2

NDL_WASH

COL2

D05

D11

C80100nF

C2410nF

VCC

A11

OE_ADC

R/W

EN_NDL_HOR

CLK_ADC

S1

SW DIP-4

1234

8765U9C

5 6

A03

9.8304MHz

A11

D09

/VPA

CHK

A02

/EN_INT/EN_CLK

D01

AUX1_NC

U7C

7406

5 6

R910K

U24

74HCT273

3478

13141718

111

256912151619

D1D2D3D4D5D6D7D8

CLKCLR

Q1Q2Q3Q4Q5Q6Q7Q8

VCC

24VDC

CLK297

CTS

R/W

LOADSENS

dis1

D11

COL2

J14

MT7/5P

12345

RY4

RG2L 24V

468

1

16

13119

SYRINGE

RIGHT

D04

D07

VIAL_SENSE

D07

COMPRESSOR

D05

Q10

3

2 1

C105100nF

-15VDC

GND

/BERR

/IACK3

D04

U3

27C2048

21222324252627282931323334353637

220

1

1918171615141312109876543

3839

A0A1A2A3A4A5A6A7A8A9A10A11A12A13A14A15

CEOE

VPP

O0O1O2O3O4O5O6O7O8O9

O10O11O12O13O14O15

A16A17

D11

C29

100nF

VCC

D01

D05

D06

NO1

VIAL_SENSE

NDL_WASH

U5

74HCT245

23456789

191

1817161514131211

A1A2A3A4A5A6A7A8

GDIR

B1B2B3B4B5B6B7B8

C104

10nF

C983.3nF*

J21

MT7/3P123

C7

C87

C97100pF

START_ADCA10

/DTACK

BUZZ

R/W

NEXT_INJ

U7E

7406

1110

U34

L6210

18

169

36

1114

27

1015

45

1213

K1K2

K3K4

A1A2

A3A4

OUT1OUT2OUT3OUT4

GNDGNDGNDGND

C43

100pF

J5

MT7/5P

12345

D13

/DTACK

/RAML

CLOCK1

D04

A01

D09

A13

SW_1

LOADSENS

C17

C74

10nF

C95

6.8uF

24VDC

PGND

/PIT

D01

D04

D03

SENS3

J24

HEADER 10X2-V

1 23 45 67 89 1011 1213 1415 1617 1819 20

J25

HEADER 7X2-V

1 23 45 67 89 1011 1213 14

J3

MT7/5P

12345

PELTIERS

+5.000V

D09

D15

D07

TRAY_HOME

COL1

D08

ALARM

NO2

RY1

RG2L 24V

468

1

16

13119

C4

R37

1K5/1W

COMMUNICATIONS

VALVE

GND

D00 D08

AUX1

NO2

C531uF

TP2

C2

Q9

3

2 1

A03

A10

D01

D04

D00

A04 A01

A01

CLK297

/IACK2

D03

D07

EN_SYRR4

26K7

TP5

C34

LOWER

/RESET

ALARM_C

24VDC

PGND

D06

NDL_DOWN

D05

D02

COL7

INH

VSGND

U37B

L293B

9

10

11 14

15

16812 13

TP4

C39

C15

T1

RFP15N05

1

23

Q8

3

2 1

R35510R

3 X 220uF/35V

GND

D09

A06

/UDS

COM4

D13

A05

OVEN_OPT

dis6

dis1

ALARM_C

C68

R29

1K5/1W

/IACK3

A06 SENS3

A10

TRAY_HOME

T2

RFP15N05

1

23

SENSOR

GND

PGND

D06

D15

D09

/CD2

D09

ALARM_NO

R1110R

47K/RS6

15

X1

9.8304MHz

1 2

GND

VCC

D12

D02

/IPL0

/OE

D06

AUX2

RN6

10K/RS9

123456789

J17

MT7/5P

12345

C61

U8

74HCT273

3478

13141718

111

256912151619

D1D2D3D4D5D6D7D8

CLKCLR

Q1Q2Q3Q4Q5Q6Q7Q8

VCC

VCC

AGND

PGND

D13

/VPA

/IPL2 /UDS

/FLASHDATA

D02

A02

NDL_UP

RV4

100K/P

U4C

74HCT04

5 6

J23

MT7/3P

123

Q2

3

2 1

U7D

7406

9 8

VCC

VCC

A06

A21

/WDOG

COM1

A04

INJ_MARK

U12

TS68000CFN

1523

272625

12131024

302928

112122

1920

6789

3233343536373839404142434445464748495051535455

543216867666564636261605958

CLKVPA

IPL0IPL1IPL2

BGACKBRDTACKBERR

FC0FC1FC2

BGVMAE

HALTRESETASUDSLDSR/W

A1A2A3A4A5A6A7A8A9

A10A11A12A13A14A15A16A17A18A19A20A21A22A23

D0D1D2D3D4D5D6D7D8D9

D10D11D12D13D14D15

C57

PS2RXE025

U7A

7406

1 2

L6

BL02

A03

A08

U6

74HCT245

23456789

191

1817161514131211

A1A2A3A4A5A6A7A8

GDIR

B1B2B3B4B5B6B7B8

C25

HOR. MOTOR

INPUTS

PH_A

A11

A10

FC1

/IPL2

CHK

RXD

/LDS

AUX1_C

RN2

10K/RS9

123456789

R302K7

KEYBOARD

G

GND

D01

D10

A07

2.4576MHz

/RESET

A07

D12

INJ2_C

J20MT7/3P

123

Q4

3

2 1

/BBUL

D03

/CD1

FANS_FAST

U25C74HCT04

56

VCC

D15

A09

DIR2 COOL_OPT

D00

R7100R

C79

2.2uF

C16

D12

BZX79C2V4

L5

BL02

VALVE

VCC

A04

D00

A03

A01

D05

TRAY_POS

Q7

3

2 1

C47100uF

GD

D03

A06

D02

/IACK1

/KEY

U33

L297

13

11

1017181920

121516

213

14

9

8

7

6

5

4SCHMCTR

EABCW/CCWCLKHF/FLRES

VSVREFOSCGND

SEN2

SEN1

D

INH2

C

B

INH1

A

D5

BZY97C15

R221R

C1010nF

U31

L6210

18

169

36

1114

27

1015

45

1213

K1K2

K3K4

A1A2

A3A4

OUT1OUT2OUT3OUT4

GNDGNDGNDGND

+5.000V

D07

/EN_CLK

D06

/BGACKD12

A11

/EN_MOT

C63

100nF

U7F

7406

13 12

PGND

GND

A12

A14

UPPER

dis6

C602.2nF*

C88

2.2nF*

C2010nF

/OE

A12

PH_D

U13

EPM7064LC68

789

15141312

54

21

6867

6059

56555452515049

1718

20 44454647

19

22232425

65646261

424140393736

27282930 32

33

57

10

I/OI/OI/O

I/OI/OI/OI/O

I/OI/O

OE2/INCLR/INOE1/INCLK/IN

I/OI/O

I/OI/OI/OI/OI/OI/OI/O

I/OI/O

I/O I/OI/OI/OI/O

I/O

I/OI/OI/OI/O

I/OI/OI/OI/O

I/OI/OI/OI/OI/OI/O

I/OI/OI/OI/O I/O

I/O

I/O

I/O

U18

TS68901Q04

19

20

222339

525124

34567

4938365037

15161718

109118

3435

2526272829303132

4142434445464748

2

X1

X2

TAITBICLK

CSDSRESET

RS1RS2RS3RS4RS5

IACKIEIINTRDTACKIEO

TAOTBOTCOTDO

SISORCTC

TRRR

I0I1I2I3I4I5I6I7

D0D1D2D3D4D5D6D7

R/W

D13

VCC

-15VDC

D08

/CD2

D04

/DTACK D00

D01

EN_FANS

FANS_FAST

R361R

AGND

+5.000V

A18

/IRQ1

D03

HEATER

AUX1

RY2

RG2L 24V

468

1

16

13119

C11

TP1

14

J1

MT6/3P

123

(EE2)

COMPRESSOR

VCC

PGND

GND

VCC

A09

COOL_OPT

INJ2_NC

C64

R32

1K5/1W

P1

DB15VF

815

714

613

512

411

310

291

VCC

VCC

+5.000V

/BBUU

/CD1

A05

D02

EN_DC_MOT

dis5

C541uF

RV22K/P

U16

ADC0809262728

12345

1216

10

97

171415818192021

252423

622

11

IN0IN1IN2IN3IN4IN5IN6IN7

REF+REF-

CLK

OEEOC

D0D1D2D3D4D5D6D7

A0A1A2

STARTALE

VCC

R34

1K5/1W

C94

10nF

C852.2uF

RN1

10K/RS9

123456789

C58

C6VCC

VCC

A12

/KEY

/DAC

R/W

D10

A14

D02

EN_VALVE

R33

1K5/1W

C560.33uF

C96100pF

GND

GND

A15

A17

RV110K/P

C33

ROTATION

VCC

/KEYSCAN

DIR1

COOL

R31

1R

U36

ULN2003

16151413121110

9

1234567

8

O1O2O3O4O5O6O7

COM

I1I2I3I4I5I6I7

GND

C13

R34K3

C45

1nF

PGND

GD

D11

/IPL1

A07

D13

/BBUL

A18

U4D

74HCT04

9 8

GND

VCC

A15

A03

dis5

D06

COOL

C65

R3933R

PSU FAN

HOME POS

Vout=0-5V

GD

VCC

D05

dis3

D6

BZY97C15

U25D74HCT04

98

A01

/IRQ1

D02

D14

C1

C21

18pF

L1

160uH/2.5A

U9A

7406

1 2

CARD

VCC

GND

FC2

D11

/IRQ2 /RESET

A09

COL7

R17

1K5/1W

R5

100R

D2

MBR1660

VCC

VCC

/BGACK

A16

FC2

D07

C44

100pF

+15VDC

GND

GND

D02

RS

C1071nF

R23

1R

12

D08

A23 A13

A05

A01

24VDC

PGND

GND

A06

/WDOG

A16

/OE

COL3

U39

ULN2003

16151413121110

9

1234567

8

O1O2O3O4O5O6O7

COM

I1I2I3I4I5I6I7

GND

J7

MT7/5P

12345

R21

1K5/1W

R241R

D1

BYV27-100

C9220uF/35V

SYR VALVE

+5.000V

24VDC

PGND

GND

24VDC

CLK_ADC

A04

PH_B

INJ_NO

U23UA7815UC

1

2

3VI

GN

D

VO

U4A

74HCT04

1 2

13

C23

68pF

TRAY MOTOR

24VDC

GND

/FEAT

A05

D03

D07

START_ADC

SCAN2

C93100uF

C32

C4133nF*

VCC

PGND

A082.4576MHz

/OE

RTS

D02

D05

COMPRESSOR

RN510K/RS6

123456

C46

U29

L298

5

6

7

10

11

12

1

15

9

4

8

2

3

13

14

INP1

ENA

INP2

INP3

ENB

INP4

CSEN1

CSEN2

VSS

VS

GND

OUTP1

OUTP2

OUTP3

OUTP4

U38

74HCT273

3478

13141718

111

256912151619

D1D2D3D4D5D6D7D8

CLKCLR

Q1Q2Q3Q4Q5Q6Q7Q8

GND

A07

/DISPL

D01

INJ2_NO

COL4

RN44K7/RS6

123456

U21REF02CP

2

4

6VI

GN

D

VO

C52100nF

C8210nF

VCC

A08

A15

/RES2

/CD1

TP3

R1 10M

A01

EN_VALVE

D13

C18

Q3

3

2 1

24VDC

D11

H/F1

H/F2

BUZZ

COL5

C72

J4

MT7/5P

12345

BUZZER

VCC

A08

/RESET

A10

CTS

D07

D00D01

D07

dis2

+

-

U20CA3130

3

2

4 8 1

6

57

U25F

74HCT04

13 12

MOTOR

VCC

PGND

FC0

/OE

EN1

D09

D02

A01

EN_FANS

D7

J2

MT7/5P

12345

VCC

/IPL0

/OE

EN_NDL_HOR

D07

DDS

EN_SYR_VALV

STOP_INP

R19

15K

J8MT7/3P

123

C810.33uF

Q5

TLC226A

3

2 1

Wednesday, August 06, 1997

31.0830.601 1

830 CPU

Spark Holland bv.

Appr. Date:

A1

1 1

Title

Size Document Number Rev

Date: Sheet of

AGND

PGND

GND

R/W

A07

D14

COL8

INJ2_NC

NO1

R633K2

U25B74HCT04

34

DDS

VCC

A04

/BBUU

/BBUL

PH_A

C14

+15VDC

GND

PGND

A05

INJSENS

INJ_NO

AUX1_NO

SCAN3

R14

15K

D4

C5

SYNC

OVEN_OPT

D08

R1327R

DISPENSER

NEEDLE

GND

+5.000V

A02

D00/OE

/DTACK/BERR

D00

SENS1

2.4576MHz

D12

COL8

D05

AUX2

AUX2_NO

D8

1N4004

J16

PCMCIA 68HDR

23456789

10111213141516

18192021222324252627282930313233

363738394041424344454647484950

52535455565758596061626364656667

17 5134 68351

D3D4D5D6D7CE1A10OEA11A9A8A13A14WE/PGMRDY/BSY

VPP1A16A15A12A7A6A5A4A3A2A1A0D0D1D2WP

CD1D11D12D13D14D15CE2

RFDHRFURFUA17A18A19A20A21

VPP2A22A23A24A25

RFU(RESET)

(WAIT)RFUREG

BVD2BVD1

D8D9

D10CD2

VCC

VCC

GN

D

GN

D

GN

D

GN

D

U22L4977A 9

14

15

3

12 5 8 10 2

11

7

6413

1

VI

VREF

VST

RIN

SST

RD

GN

D

FC CO

SC

FI

OUT

BT

RO

UT

SYN

C

RO

SC

J12

HEADER 13X2-V

1 23 45 67 89 1011 1213 1415 1617 1819 2021 2223 2425 26

GND

A13

A22

D12

A08

FC0

D03

/PIT

A13TXD

D01

/RESET

EN_TRAY

RS

J6

MT7/5P

12345

C102

10nF

OUTPUTS

VCC

24VDC

A09

A14

D00

D02

PB RST

TD

TOL

ST

RST

RST

U14

DS1232

1

2

3

7

6

5

P2

DB9VF

594837261

C382.2uF

+15VDC

AGND

EN1

/IPL1

/RESET

A03

/ROM

SW_2

C73

PGND

A07

A11

A01

D07

SENS4

/ROM

D12

R26

1K5/1W

GND

A06

H/F2

AUX1_NO

C66

D14BZT03C12

R2220R

C40

2.2nF

(EE1)

MOTOR

GND

A02

R/W

A03

D06

FREEZE_INP

COL6

AUX1_C

RN3

4K7/RS6

123456

R18

1K5/1W

C42

1nF

PGND

D10

EN_TRAY

D05

/BBUU

/COMBO

/IRQ2

D01

LOADSENS

R271R

C37

100pF

SYRINGE

GND

D14

/IACK2

/BR

TXD

R/W

D01

U35

L297

13

11

1017181920

121516

213

14

9

8

7

6

5

4SCHMCTR

EABCW/CCWCLKHF/FLRES

VSVREFOSCGND

SEN2

SEN1

D

INH2

C

B

INH1

A

C108DSS306-2200pF

PGND

GND

STOP_INP

OE_ADC

INJ_NC

ALARM_NO

C9010uF

GND

GND

-15VDCD13

D01

A04

A17

D15

24VDC

PH_C

A02

A22

A03

/EN_MOT/WDOG

/RESET

INJ_NC

AUX2_NO

C103

10nF

R8

15K

GD

D14

R/W

COM8

INJSENS

/FEAT

L3

BL02

LEFT

PGND

VCC

A01

A14

D15

AUX2_C

R15

4K7

GND

A14

D05

EN_NDL_VER

/RESET

NDL_UP

SYNC

D06

dis0

dis3

C62

4.7uF*

C12

L4

40uH/8A

C31

R41

220R

U1

6264

109876543

25242123

2

1202722

1112131516171819

26

A0A1A2A3A4A5A6A7A8A9A10A11A12

A14CSWEOE

D0D1D2D3D4D5D6D7

A13

COOLING

PGND

AGND

GND

DIR1

A02

A09

/IRQ3

D06 EN_SYR_VALV

A16

D04

C91470uF/35V

J11

MT6/5P

12345

U4B

74HCT04

3 4

VCC

GND

/BR

/IRQ3

D03

D14

FREEZE_INP

/RES2

D10

SENS4

R38

1R

C77

2.2uF

C55

R16

18K

VCC

A12

/CTRL_IO

D02 COM1

RXD

NDL_LEFT_RIGHT

D04 INJSENS

dis7

INJ_C

VCC

A05

COM8

COM2

/CTRL_IO

R40

100R

A02

/ROM R/W

EN_SYR

INJ2_C

U11

MK48Z02

87654321

232219

182021

910111314151617

A0A1A2A3A4A5A6A7A8A9A10

CEOEWE

D0D1D2D3D4D5D6D7

J9

MT7/10P

12345678910

C69

RN710K/RS6

1 2 3 4 5 6

MEMORY

A23

COM4

RTS

D04

D01

D03

dis7

C7010nF

C49

1nF

C84

390pF

U7B

7406

3 4

U2

6264

109876543

25242123

2

1202722

1112131516171819

26

A0A1A2A3A4A5A6A7A8A9A10A11A12

A14CSWEOE

D0D1D2D3D4D5D6D7

A13

INJECTOR

GND

/DAC

D11

A06

CLOCK2

D03

COL8

+

-

U19CA3130

3

2

4 8 1

6

57

D3

C89

10nF

C83

22nF*

U9E

7406

11 10

C8

100nF

U4F

74HCT04

13 12

AGND

VCC

UPPER

R/W

D11

NEXT_INJ

dis2

D9

1N4004

D10

1N4004

C75

Q6

3

2 1

BZ1

CPM121A0A

SENSORS

GND

A09

/AS

SCAN3 SW_2

EN_NDL_VER

D04

LOWER

/OE

ALARM_NC

EN_DC_MOT

J15

MT7/5P

12345

47K/RS6

16

C36

100nF

U9B3 4

91

Tray needle Sensor PCB 0830.602 comp. view

Keyboard PCB 0830.603

93

Multilink serial interface PCB 0725.625

C2

1uF/63V

S1

SW DIP-4

1234

U1D

74HCT08

12

1311

S2

SW DIP-4

1234

CONNECTED

Com3

VCC

GND

Com1

COM3

VCC

GND

Tx_1

U1B

74HCT08

4

56

U1C

74HCT08

9

108

TO PC

C7 1uF/63V

Com0

ACE or HPD

VCC

Com0

AUTOSAMPLER

TO HPD/ACE

Rx_2

Communication board installed in

Com0

Communication board installed in

SYNC. LINESCONECTED TO

PC

Com1

GND

Tx_int

GND

C64.7uF/63V

C4 4.7uF/63V

GND

C1

22uF/63v

S1 S2

DIP1: OFF DIP1: OFF

DIP2: OFF DIP2: ON

DIP3: OFF DIP3: ON

DIP4: OFF DIP4: OFF

C5

10nF

IF NO REQUREMENTSNEEDED

C3

10nF

S1 S2

DIP1: ON DIP1: ON

DIP2: ON DIP2: OFF

DIP3: ON DIP3: OFF

DIP4: ON DIP4: ON

Rx_1

SYNC.LINES

OFF

ONLY AS CONTROL

LINES ON

Rx_int

U1A

74HCT08

1

23

COM2

VCC

S1 S2

DIP1: ON DIP1: ON

DIP2: ON DIP2: ON

DIP3: ON DIP3: ON

DIP4: ON DIP4: ON

J1

HEADER 10X2

1 23 45 67 89 1011 1213 1415 1617 1819 20

GND

U2

MAX240

43

35412

36

42

18

19

64405

8

177

10

30

2625

2728

29

15161314

38393

37

SHDN

R5IT5OT5I

R5O

EN

GND

VCC

T3OR3IR4IT2O

T20

R1IT10

R2I

V-

V+C1+

C1-C2+

C2-

T1IR10R2OT2I

T4IR4OR3OT3I

Com2

Rx_int

C8 1uF/63V GND

S1 S2

DIP1: ON DIP1: ON

DIP2: ON DIP2: OFF

DIP3: ON DIP3: OFF

DIP4: ON DIP4: ON

TO PC

Tx_int

SYNC. LINESSWITCHED

OFF

Tx_2

CONNECTED

S1 S2

DIP1: ON DIP1: ON

DIP2: OFF DIP2: OFF

DIP3: ON DIP3: OFF

DIP4: OFF DIP4: ON

CONNECTED

Date post:	14-Feb-2018
Category:	Documents
Upload:	hathuan
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