1. 1. CONSIDERSAFETY: PreparingSamplesforCryopreservation
2. 2. LOWTEMPERATUREPROTECTION Whenhandlingcryopreservedsamplesandcold temperatures,stashouldwear: Paddedgauntletsforhand/armprotection Long-sleevedlabcoats Closed-toefootwear Afull-facevisor
3. 3. SAMPLEPREPARATION Additivesareaddedtocellswhenpreparingthemfor cryopreservationtoprotectthecellsfromdamage: dimethylsulfoxide(DMSO) polyethyleneglycol glycerol DMSOiseasilyabsorbedthroughskin.Long-sleevedlabcoats, faceprotectionandglovesshouldbewornduringpreparation.faceprotectionandglovesshouldbewornduringpreparation.
4. 4. BIOHAZARDSANDCRYOPRESERVEDSAMPLES Whendealingwithbiosamples,stashouldtakethefollowing precautions: Beawareofthebiosafetylevels Properlylabelsamples Practicetheappropriatesafetyproceduresforthegiven samples
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6. 6. CONTAMINATION Pathogenscansurvivethelowtemperatures.Itisimportant towipetheoutsideofvialswithgauzesoakedinalcohol beforeopening. Wherecontaminationissuspected,stashouldfollow appropriatedecontaminationproceduresforcleaning liquidnitrogenstoragetanks.
7. 7. SOURCES ConsiderSafety:PreparingSamplesforCryopreservationBlog Shutterstock