FINAL TECHNICAL REPORT
Construction of an Integrated Database on Biosafety and use of GMOs in India
Principal Investigator : Dr. T. R. Sharma
Coordinator : Prof. K.R. Koundal
Co- Investigators:Dr. K. C. BansalDr. N. K. SinghDr. Kishore Gaikwad
National Research Center on Plant Biotechnology
IARI, New Delhi-110012
www.nrcpb.org Email: [email protected]
•Construction and Updation of the Database•Retrieval, storage and management of data on biosafety of GMOs•To make GM Crops database public through a dedicated Web site •To impart training to the Scientists and Technical Staff on Biosafety web resources
Objectives of the Project
Relational Diagram Showing Relationship Among Different Tables
WEB-SERVER
(SUN ULTRA10)
COMPUTE-SERVER
(SUN V240)
Enter query Authentication
Outputs
US
ER
INT
ER
FA
CE
Gene
Primary Database
(Transgenics)
Primary Database(GM_Pub)
Promoter
Crop Env_haz
Health_haz
Soil_micr
Develo-per
QUERY INTERFACE
Inputs
MID
DLE
WA
RE
MySQL
APPL-SERVERJSP ENGINE
HTML – DHTML -FLASH
JAVA - JDBCResults
Query
MySQLMySQL
Basic Flow Chart of the NRCPB Database
www.nrcpb.org GM Crop Database
www.nrcpb.orgClick here to
access GM crop database
Availability of Database on WWW
GM Crop Database Home Page
GM Crop Search Database Interface
GM Crop Search Database Interface
Transgenic Line Query Interface
Transgenic Line Query Interface
Result Page for the Query Submitted
Transgene-Crop-Promoter Query Interface
Result Page for the Query Submitted
Promoter Info Query Interface
Result Page for the Query Submitted
Developer Institution Query Interface
Result Page for the Query Submitted
CropNumber of transgenic line
Number of genes
Number of promoter Developer
Cotton 137* 4** 1*** 20
Rice 4^ 5^^ 2^^^ 1
* Brinjal - 1% 1*** 1
* Tomato - 1# 1*** 1
* Potato - 2& 2 && 1
* Chickpea - 1+ 1*** 1
* Corn - 3^^^^ - -
Current status of information stored in transgenics database
* 58 Bt cotton hybrids approved for commercial planting in India till date, rest are released for field trials in different zones.
** (cry1Ac,cry1Ab,cry2Ab,Vip3A), *** (CaMV 35S)^ (SGR1, SGR2, LLRICE06, LLRICE62), ^^ (Psy, crtI, lyc, Zea mays psy, Bar)^^^ (Ubi-1 promoter, GT1 promoter), ^^^^ (cry1F,cry1X, CP4EPSPS)# (Rep gene)& (Ama1, RGA2), && (CaMV 35S, GBSS)+ (Cry1Aa3), % (Cry1Ab)* Transgenic crops under development and field trials
GM Publication Query Interface
Result Page for the Query Submitted
Result Page for the Query Submitted
Result Page for the Query Submitted
Year
Biosafety & IPR
Issues
Food &
Feed
Risks & Benefit
s
Environment & Health
Concern
Bt Cotton RiceSoy bean Brinjal Tomato Corn Potato Canola
Soil Micro flora
Selectable
MarkerOthers
2006-07 2 - - - - - - 40 6 7 28 17 19 - - -2005 14 5 6 6 7 4 5 25 4 7 15 13 12 2 3 32004 3 10 4 6 10 2 2 26 0 7 1 13 10 8 1 42003 5 7 10 10 13 6 1 20 1 8 1 9 8 8 5 82002 3 6 8 3 4 6 3 13 0 2 0 8 12 4 1 52001 4 5 8 8 5 5 4 12 0 3 1 9 7 3 0 92000 7 5 3 4 8 6 1 14 0 2 1 7 5 3 1 91999 2 1 1 1 3 0 2 7 0 3 0 3 5 1 1 41998 1 0 0 0 3 0 2 5 0 5 2 2 7 0 0 21997 0 0 1 1 1 1 2 0 0 2 1 0 1 0 0 1Total 41 39 41 39 54 30 22 162 11 46 50 81 86 29 12 45
Total number of records = 788
Current status of information stored in GM-Publication Database- June 2007
1. Indian Agricultural Research Institute 2. National Research Centre on Plant Biotechnology
3. Mahatma Phule Krishi Vidyapeeth4. National Bureau of Plant Genetic Resources5. Tata Energy Research Institute, TERI6. NRC for Sorghum7. Jawaharlal Nehru Krishi Vishwa Vidyalaya8. Tamilnadu Agricultural University9. Central Food Technological Research Institute10. Israel Life Science Industry11. Vasantdada Sugar Institute, Pune12. Centre for DNA Fingerprinting and Diagnostics13. Kerala Agricultural University14. Cornell University15. Central Rice Research Institute16. Dr. Babasaheb Ambedkar Marathwada University
17. Dr.Balasaheb Sawant Konkan Krishi Vidyapeeth18. Rajasthan Agricultural University, Bikaner19. Dhaka University20. Reuters India21. Central Institute for Cotton Research
22. Sathyabama University23.Osmania University24. Central Research Institute For Jute & Allied
Fibres25. Bishop Heber College, Trichi, TAMILNADU26. Amity Institute of Biotechnology27. Birla Institute of Technology28. Ministry of Environment and Forests -29. University of Agricultural Sciences,
BANGALORE30. Gauhati University31. University of Agricultural Sciences Dharwad32. Kerala university33. Directorate of Rice Research34. Junagadh Agricultural University35. Punjab State Council for Science & Technology36. Sher-e-Kashmir University of Agricultural
Sciences37. IARI Regional Station, Pusa, Bihar38. Central Soil Salinity Research Institute39. National Research Centre for Rapeseed
Mustard40. Central Institute of Freshwater Aquaculture41. Apeejay institute of Management42. Delhi University
List of Important Users of DB
Total Number of Hits in the Database = 2450
1. Crop Design2. Monsanto3. Tranzpac4. Emerging Solutions
5. JK-Agrigenetics Ltd6. GrasslanD Technology7. MAHYCO
Feedback Query Form
User`s Feedback
National Training Workshopson
Biosafety Issues and Web Resources in GMOs
Dr. R. K. KatiyarCourse Director &DirectorNational Research Centre on Plant Biotechnology, IARI, New Delhi –110012Email:[email protected]
Dr. K. R. KoundalCourse Co-Director &Joint Director ResearchIndian Agricultural Research Institute, New Delhi -110012Email:[email protected]
Dr. T. R. SharmaCourse CoordinatorNational Research Centre on Plant Biotechnology, IARI, New Delhi –110012
Objective 4
First Training : April 23-25, 2007Second Training : May 14-16, 2007Third Training : June 7-9, 2007
Numbers and Period of Trainings
Participants
Total No. of Participants = 45ICAR Institutes Represented = 18SAUs represented = 13
Resource personsICARDBTDUCSIRSAUsICRISATMOEFBCIL
About the Participants and Resource Persons
Major Areas Covered in the training
•Biosafety issues Related to GMOs •GM crops development and their post release management •GMO detection in food feed and seeds•Cartagena protocol & safe trans boundary movements of GMOs•Environmental and health concerns related to GMOs •Regulatory issues and regulatory agencies•Availability and use of Web Resources on GMOs etc.•Practical on Web based Resource Utilization
Training Manuals Developed
A Technical Bulletin
Some Events of the 1st Training Workshop
Some Events of the 2nd Training Workshop
Some Events of the 3rd Training Workshop
Participants Feed Back about the Training
Outcome of the Project
A Database: A web based Comprehensive database on Biosafety Issues related to GMOs
Publications1. Sharma, T.R. K Srivastava, S.Singh and K.R. Koundal. 2006. Development of an Integrated Database on Biosafety and use of GMOS in India. Proc. Int Conf Implications of the Cartagena Protocol on Biosafety, Org by: Ministry Environment and Forests, Govt. of India. New Delhi .169-186.2. Koundal K.R. and T.R. Sharma. 2006. Biosafety concerns associated with GMOs. Proc. Int Conf Implications of the Cartagena Protocol on Biosafety, Org by: Ministry Environment and Forests, Govt. of India. New Delhi .83-893. Sharma,T.R., K. Srivastava, S. Singh, A. Kumar, K. Gaikwad, K.C. Bansal, N.K. Singh and K.R. Koundal.2007. Web based Information Resources on GMOs. In CRTAGENA Potocols on Biosafety:Decisions to Diagnostics.(Eds Ramdhawa et l.2007)NBPGR,New Delhi 179p.
Technical Bulletin
Sharma TR . 2007. GM Crops Database: An Interactive Web Resource. Pp. 16.
Training Manuals1.Sharma TR, KR Koundal, RK Katiyar 2007. 1st National Training Workshop on Biosafety Issue and Web Resources. April 23-25.pp682.Sharma TR, KR Koundal, RK Katiyar 2007. 2nd National Training Workshop on Biosafety Issue and Web Resources. May 14-16.pp76.3.Sharma TR, KR Koundal, RK Katiyar 2007. 3rd National Training Workshop on Biosafety Issue and Web Resources. June 7-9. pp80
Infrastructure Developed under the Project
•SUN FIRE V240 SERVER SYSTEM : One number•Storage Data Workstations : Two numbers.
Name of the personnel Period Present position
Research Associates
Ms Keerti Srivastava
Mr. Ajay Kumar Mehato
28-3-2005 to 30-9-06
1-10-2006 to 30-6-2007
Joined in IT Company
Tomato Genome Seq
ProjectSenior Research Fellows
Ms Sangeeta Singh
Ms.Charu Singh
Mr. N. Reddy
15-4-2005 to 30-6-2007
2-3-2007 to 30-62007
20-3.2007 to 8-6-2007
Pigeonpea Genome Project
ICAR Network Project on
Transgenic Crops
Joined in Ph D
Developed Trained Human Resource in Bioinformatics
Proposed work for the 2nd Phase
Updating the Database.
To develop a Network of Centres for Making Database in Regional Languages (Hindi, Punjabi, Tamil, Telgu and Kanada).
Developing a simple version of the database for the farming community and common public.
Acknowledgements
Financial AssistanceMinistry of Env. & Forest, GOIGEF- World Bank
Prof. K.R. KoundalJoint Director Research, IARI
Dr. PA KumarProject Director, NRCPB
KeertiAjaySangeetaCharu