. . . III

PLANT CELL BIOLOGY SPECIAL ISSUE OF CELL BIOLOGY INTERNATIONAL REPORTS

Vol. 13, No. 1, January 1989 Edited by E. L. Vigil and C. Hawes

Contributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . E. L. Vigil - Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . G. I. McFadden. ln situ hybridisation in plants: from macroscopic to

ultrastructural resolution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . N. Harris, H. Grindley, J. Mulchrone and R. R. D. Croy. Correlated in

situ hybridisation and immunochemical studies of legumin storage protein deposition in pea (Pisum sativum L.) . . . . . . . . . . . . . .

E. M. Herman, M. J. Chrispeels and L. M. Hoffman. Vacuole accumu- lation of storage protein and lectin expressed in transgenic tobacco seeds . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

E. L. Vigil, L. C. Frazier and C. Pooley. Effect of drought stress on pro- tein body development in radicles of cotton embryos: a quantitative study with image analysis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

C. Sautter. lmmunolocalization studies in the biogenesis of plant microbodies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

C. Sautter. Fate of glyoxysomal malate dehydrogenase from waterme- lon after heterologous in viva translation in Xenopus oocytes

R. P. Donaldson and E. Gonzalez. Glyoxysomal membrane proteins are present in the endoplasmic reticulum of castor bean endo- sperm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

S. M. Wick, S. Cho and A. R. Mundelius. Microtubule deployment within plant tissues: fluorescence studies of sheets of intact mesophyll and epidermal cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

L. Qiao, F. Grolig, P. P. Jablonsky and R. E. Williamson. Myosin heavy chains: detection by immunoblotting in higher plants and localization by immunofluorescence in the alga Chara . . . . . . . . . . . .

C. Hawes, J. Coleman, D. Evans and L. Cole. Recent advances in the study of plant coated vesicles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Y. Mineyuki, D. S. Letham and C. H. Hocart. New 3-substituted xanthines: potent inhibitors of cell plate formation . . . . . . . . . . . . . . . . .

F. Gubler. lmmunofluorescence localization of microtubules in plant root tips embedded in butyl-methyl methacrylate . . . . . . . . . . . . . . . . . . . . . . . . . . . .

G. I. Cassab and J. E. Varner. Tissue printing on nitrocellulose paper: a new method for immunolocalization of proteins, localization of enzyme activities and anatomical analysis . . . . . . . . . . . . . . ..a..........

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CELL BIOLOGY International Reports

Vol. 13, No. 2, February 1989

H. S. Brand, G. P. K. van Kampen, R. J. van de Stadt, R. Kuijer and J. K. van der Korst. Effect of sulfate concentration on glycos- aminoglycan synthesis in explant cultures of bovine articular cartilage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

S. Spisani, R. Gavioli, P. Chiozzi, F. Lanza, F. Bortolotti and S. Traniello. Lymphocytes treated with natural alfa-interferon produce a chemotactic factor for human neutrophils . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

G. Y. Hu, J. E. Novakofski and H. J. Mersmann. Lipid, metabolism of rat adipocytes in culture . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

A. M. Rinaldi and I. Salcher-Cillari. Studies of the mechanisms of nuclear control over the synthesis of mitochondrial DNA in sea urchin eggs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

L. D. Apelgren and T. F. Bumol. Biosynthesis and secretion of throm- bospondin in human melanoma cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

H. Senoo, Y. Tsukada, T. Sato and R. Hata. Co-culture of fibroblasts and hepatic parenchymal cells induces metabolic changes and formation of a three-dimensional structure . . . . . . . . . . . . . . . . . . . . . . .

A. Dieckmann-Schuppert and R. M. Franklin. Compounds binding to cytoskeletal proteins are active against Plasmodium falcipa- rum in vitro . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . , . . . . . .

J. M. Jamison, D. G. Flowers, S. Kitareewan, K. Krabill and C. Tsai. Potentiation of the antiviral activity of poly r(A-U) by ribo- flavin, FAD and FMN . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Short Communications J. T. Emerman, D. Wilkinson and Y. Hollander. Collagen accumulation

by human mammary epithelial cells in primary culture . . . . . . . . . T. J. Smith, J. I?. Powell and M. S. Fuchs. Selective expression of phos-

phodiesterase II during ovarian development in Aedes aeg ypti . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Book review . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Announcements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

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CELL BIOLOGY International Reports

Vol. 13, No. 3, March 1989

C. Verger, M. Petrovic and J. Imbenotte. Stimulatory effects of protein synthesis inhibitors on the spreading rate of 3T3 cells . . . . . . . . . . . .

M. F. W. te Pas, J. Boonstra, L. Havekes, S. C. Hesseling and M. Ponec. The competence of transformed keratinocytes to differentiate is accompanied by amplification of the LDL- and EGF- receptor genes but not of the insulin receptor gene . . . ..**.........

H. Tokuyama and Y. Tokuyama. Bovine colostric transforming growth factor-p-like peptide that induces growth inhibition and changes in morphology of human osteogenic sarcoma cells (MG-63) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

A-M. Makower, J. Wroblewski and A. Pawlowski. Effects of IGF-I, rGH, FGF, EGF and NCS on DNA-synthesis, cell proliferation and morphology of chondrocytes isolated from rat rib growth cartilage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

S. Chattergee. Pinocytosis in heterospecific amoebae . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . E. Bednarska and A. Tretyn. Ultrastructural localization of acetylcho-

linesterase activity in the stigma of Pharbitis nil . . . . . . . . . . . . . . . . . . . . . . A. Cuadrado, M. H. Navarrete and J. L. Canovas. Exponential pattern of

cell age distribution in dividing cells of plant meristems . . . . . . . . H. Kamei. Nuclear granules recognized by some monoclonal antibo-

dies against intermediate filament protein locate on chromo- somes during mitosis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

V. VBtviEka, P. Rossmann, M. Bilej, I. Miler, E. Knobloch and P. Sims. ln viva interaction of bilirubin with the cells of the immune sys- tem in mice: an ultrastructural electronmicroscopic study . . . . .

A. Wagle, S. Desai and M. G. Deo. lmmunohistochemical localization of ‘Enhancing factor’, a novel growth modulator, in the mouse small intestines . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Announcements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .._.._.__..............................................

CELL BIOLOGY International Reports

Vol. 13, No. 4, April 1989

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Page Editorial . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 315 Z. Baolu, L. Xiaojie, H. Rungen, J. Weyning and X. Wenjuan. ESR studies

on oxygen consumption during the respiratory burst of human polymorphonuclear leukocytes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 317

R. A. Harper. Keloid fibroblasts in culture: abnormal growth behaviour and altered response to the epidermal growth factor . . . . . . . . . . . . . . . 325

M. Bilej and V. Vetvicka. Quantitative evaluation of changes in the microtubulecontent of lymphocytes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 337

vi

C. Pellicciari, G. Mazzini, A. M. Fuhrman Conti, L. De Grada and M. G. Manfredi Romanini. Effect of hypertonic medium on human cell growth: III. Changes in cell kinetics of EUEcells . . . . . . . . . . . . . . . . . 345

A. F. Brown, V. Dugina, G. A. Dunn and J. M. Vasiliev. A quantitative analysis of alterations in the shape of cultured fibroblasts induced by tumour-promoting phorbol ester . . . . . . . . . . . . . . . . . . . . . . . . . . . 357

J. Gavrilovic and G. Murphy. The role of plasminogen in cell-mediated collagen degradation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 367

J. Kajstura and J. Bereiter-Hahn. Loss of focal contact accompanies the density of dependent inhibition of cell growth . . . . . . . . . . . . . . . . . . . . . . . . . . 377

M. Stopp, J. Grof3 and H.-Ch. Lauer. Protein content in rabbit periodontal ligament fibroblasts during growth in culture . . . . . . . . . . . . . . . . . . . . . . . . . . 385

F. Arezzo. Sea urchin sperm as a vector of foreign genetic information . . . . 391 Announcement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 405

CELL BIOLOGY International Reports

Vol. 13, No. 5, May 1989

Editorial . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . A. Dieckmann-Schuppet-t and R. M. Franklin. Compounds binding to

cytoskeletal proteins are active against Plasmodium falcipa- rum in vitro . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

L. C. Fowke, M. A. Tanchak and P. J. Rennie. Serial section analysis of coated pits and coated vesicles in soybean protoplasts . . . . . . . . . .

T. Makita, H. Ichimal, S. Kagabu, K. Manba, I. Naito, R. Lindblom, M. Hatsuoka and T. Okawa. Scanning and transmission electron microscopy of biodegradable microspheres (DSM) in blood vessels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

R. Evangelisti, G. Stabellini, E. Becchetti and P. Carinci. Exogenous gly- cosaminoglycans modulate extracellular and intracellular accumulation of newly synthesized glycosaminoglycans by embryonic chick skin fibroblasts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

M. Leclerc and G. Luquet. ln vitro effect of silica on axial organ cells in the presence and absence of mitogens . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

I. Yanez and M. O’Farrell. Variation in the length of the lag phase following serum restimulation of mouse 3T3 cells . . . . . . . . . . . . . . . . . . .

T. Taketomi, M. Motono and M. Miyawaki. On the biological function of the mandibular gland of decapod crustacea . . . . . . . . . . . . . . . . . . . . . . . .

J. D. I. Harper, J. M. Mitchison, R. E. Williamson and P. C. L. John. Does the autoimmune serum 5051 specifically recognise microtubule organising centres in plant cells? . . . . . . . . . . . . . . . . . . . . . . . . .

Book review . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Reports from Cell Biology Societies . . . . . . . ..__..__................_....__......................

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CELL BIOLOGY International Reports

Vol. 13, No. 6, June 1989

R. O’Neill and I. Hickey. Analysis of APRT deficient mutants of friend erythroleukaemia cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

J. Radford and I. R. Radford. Fliv-1: a surface membrane differenti- ation antigen of fetal rat hepatocytes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

G. 0. Wasteneys, P. P. Jablonsky and R. E. Williamson. Assembly of purified brain tubulin at cortical and endoplasmic sites in perfused internodal cells of the alga Nitella tasmanica . . . . . . . . . . .

B.-L. Zhao, X.-J. Li and W.-J. Xin. ESR studies on active oxygen radi- cals produced in the respiratory burst of human polymopho- nuclear leukocytes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

A. Casale. Use of electrophoretic techniques and specific antibodies to analyse tubulin isotypes in rabbit testis and spermatozoa . . . . . .

T. Okawa, H. Ichimal, T. Ishida, M. Kawata, S. Kaguba, K. Mamba and T. Makita. Implication of activation of intraperitoneal macro- phages with bio-degradable microspheres . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

W. D. Norris, G. Johnson and G. S. Donald. The effect of minoxidil on endothelial cells in vitro . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

P. Kovacs, Y. Okano, Y. Nozawa and G. Csaba. Insulin imprinting of neuroblastoma cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Y. Kusama, J. Enami and Y. Kano. Growth and morphogenesis of mouse prostate epithelial cells in collagen gel matrix culture . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Book review . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Announcements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

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CELL BIOLOGY International Reports

Vol. 13, No. 7, July 1989

Page P. J. Dierickx. Partial purification and characterization of the soluble

glutathione transferase isoenzymes from cultured Hep G2 cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 585

S. Miscia, A. Cataldi and P. M. Grimley. Influence of phosphatidylcho- line on two protein mRNA levels in interferon treated Daudi cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 595

A. Y. Alexandrova, V. I. Samoilov and J. M. Vasiliev. Distribution of platelets on flat substrata . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 601

J. Boonstra and M. Ponec. Proliferating, transformed keratinocytes cul- tured under low Ca2+ conditions exhibit high-affinity epider- mal growth factor receptors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 609

. . . VIII

S. S. -L. Li and E. W. Hou. Estrogen-induced expression of mouse lac- tate dehydrogenase-A gene . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

C. Mothersill, A. Cusack. C. B. Seymour and T. P. Hennessy. Optimi- sation of media for the culture of normal human epithelial cells from oesophageal mucosa . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

W. Lee, A. Newman and V. Honribia. HRP labelling of afferent vesti- bular nerve fibers in mammals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

P. A. Wackym, P. E. Micevych, P. H. Ward and F. H. Linthicum. Trans- mission and immunoelectron microscopy of celloidin- embedded human temporal bones . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

I. Baines and C. A. King. Demonstration of actin in sporozoites of the protozoon eimeria . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

E. J. Mitchell and L. H. Martin. Evidence for a myosin gene in Tetra- hymena . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Letter to the Editor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Book review . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Announcements and meetings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

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CELL BIOLOGY International Reports

Vol. 13, No. 8, August 1989

Page A-M. Makower, A. Skottner and J. Wroblewski. Binding of insulin-like

growth factor-l (IGF-I) to primary cultures of chondrocytes from rat rib growth cartilage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

E. Wyroba. Beta-adrenergic stimulation of phagocytosis in the unicellu- lar eukaryote paramecium aurelia . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

I. Baines and C. A. King. Demonstration of actin in the protozoon - Gregarina . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

S. Kanda, K. Nomata, P. K. Saha, N. Nishimura, J. Yamada, H. Kanatake and Y. Saito. Growth factor regulation of the renal cortical tubular cells by epidermal growth factor, insulin- like growth factor-l, acidic and basic fibroblast growth factor, and transforming growth factor-pin serumfreeculture . . . . . . . . . . . . . .

H. Toutain, N. Vauclin-Jacques, J-P. Fillastre and J-P. Morin. Isolation of a pure suspension of proximal tubular cells from the rab- bit kidney: morphological, biochemical and metabolic assessment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

G. Lizard, Y. Chardonnet, M-C. Chignol, D. Schmitt, M. Lenne and J. Thivolet. Ultrastructural characteristic of hybrids derived from normal and hand wart keratinocytes after serial passages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

European Tissue Culture Society Meetings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Constituent Cell Biology Societies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

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Vol. 13, No. 9, September 1989

J. M. Bentel, A. W. J. Lykke and G. J. Smith. Cloned murine non- malignant, spontaneously transformed and chemical tumour- derived cell lines related to the type 2 pneumocyte . . . . . . . . . . . . . . . . .

T. Matsuno. Oxidation of cytosolic NADH by the malate-aspartate shuttle in MC29 hepatoma ceils . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

D. Fais, A. N. Prusov and V. Yu Polyakov. The anchorosome, a special chromatin granule for the anchorage of the interphase chromosome to the nuclear envelope s................,.......,........,....

S. P. Guy, T. K. Bradshaw and R. F. Itzhaki. Chromatin characteristics of carcinogen-treated avian erythrocyte nuclei and effects of reactivation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

J. H. Clarke, J. A. Norman and E. Lavery. Some observations on conta- mination of animal cell cultures by the fungus Aspergillus fumigates and suggested control measures . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

G. W. Ireland, P. J. C. Dopping-Hepenstal, P. W. Jordan and C. H. O’Neill. Limitation of substratum size alters cytoskeletal organization and behaviour of Swiss 3T3 fibroblasts . . . . . . . . . . . . . .

C. Nastruzzi, G. Feriotto, R. Barbieri, R. Ferroni, M. Guarneri and R. Gambari. Induction of murine erythroleukaemia cell differentiation by proteinases is inhibited by aromatic poly- amidines . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

J. Kovacs and A. P. Karpati. Regression of autophagic vacuoles in mouse pancreatic cells: a morphometric study of the effect of methylamine and chloroquine followed by cycloheximide treatment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Short Communication A. R. Eynard, P. Quiroga and R. Silva. Dietary fatty acid composition

modulates the metastatic behaviour of a murine mammary gland adenocarcinoma . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Book Review . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Society Meetings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

CELL BIOLOGY International Reports

Vol. 13, No. 10, October 1989

Editorial and Erratum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . P. Wilson and A. Forer. Identifying the site of microtubule poly-

merization during regrowth of UV-sheared kinetochore fibres

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using antibodies against acetylated alpha-tubulin . . . . . . . . . . . . . . . . . . . 823

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N. Zagris, M. Panagopoulou and V. Anastassopoulos. Extracellular matrix organized in embryonic cavities during induction of the embryonic axis in chick embryo B.........................................

M. I. Gimenez-Abian, C. de la Torre, J. F. Lopez-Saez and J. L. Canovas. Rates of cell entrance into different periods of the cell cycle sustain that negative exponential cell age distri- bution occurs in Allium cepa L. meristems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

L. Romano. Comparative aspects of sulfate transport in the erythro- cytes of different mammalian species . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

P. E. Hedley, A. M. Dalin and W. Engstromm. Developmental regu- lation of insulin like growth factor II gene expression in the Pig . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

M. I. Morasso, M. S. Rieber, C. Urbina and M. Rieber. Changes in cytoskeleton-associated molecules in cells with different degrees of proliferation and metastatic ability . . . . . . . . . . . . . . . . . . . . . . . . .

Y. Nagao, R. Tamatani, Y. Kawarai and K. Nishikawa. Immunohistoche- mica1 localizations of albumin and transferrin accumulated in Rhodamine fibrosarcoma tissue of rats for supporting growth of the tumor cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

J. Wegrowski, G. Bellon, B. Haye and J. P. Borel. Effects of thyroid- stimulating hormone and phorbol ester on glycosaminogly- can synthesis in porcine thyroid epithelial cells in primary culture . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

G. A. Homandberg, B. M. Dunn, D. M. Grant, B. Schumacher, D. M. Bartley and R. Eisenstein. Synthetic peptides of the amino- terminus of fibronectin inhibit endothelial cell growth . . . . . . . . . . . .

Announcements and Society Meetings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

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Page T. Kubin, M. Yanagida, S. Mori, Y. Hayashi, E. Gohda and

I. Yamamoto. Inhibition of DNA synthesis of adult rat hepatocytes in primary culture by dibutyrylcytidine 3’, 5’-cyclic monophosphate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

L. Nitsch, E. Gionti, R. Cancedda and P. C. Marchisio. The podosomes of Rous sarcoma virus transformed chondrocytes show a peculiar ultrastructural organization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

M. Ohoue and T. Makita. The formation of multivesicular structures in the adipose cells of chickembryos . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

H. M. W. van der Velden, P. P. G. S. Starmans, M. J. Classens, I. G. van Meurs and F. Wanka. Induction of DNA replication in mamma- lian Gl nuclei by a heat-inactivible component from permeable

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S phase cells; an assay system . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 933

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N. S. White, P. M. Lackie and D. M. Shotton. Imaging of immunogold labelled antigens on capping thymocytes by confocal reflection contrast scanning optical microscopy a.........................................

M. Goldfinger, F. Hanocq and R. Tencer. Presence of high molecular weight glycans on the surface of germ and embryonic cells of Xenopuslaevis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

I?. Stewart, J. Nelson and D. J. Wilson. Epidermal growth factor promotes chick embryonic angiogenesis . . . . . . . . . . . . . . . . . . . . . . . . . . . . ..~......................

Short Communications P. J. Dierickx. Identification of soluble glutathione transferases in the rat

hepatoma derived established Fa32 cell line ,............................... Zhou Ju-Hua and Liang Hai-man. Effects of 2,4-dichlorophenoxyacetic

acid (2,4-D) and a-napthalene acetic acid (NNA) on the control of the cell-cycle of Daphne odora stem callus . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

J. Kimura, A. Tsukise, N. Okano and K. Sato. Cytochemistry of glycocon- jugates in the hamster Zona pellucida associated with oocyte maturation . . . . .._........._............._................._....................................

Announcements and Society Meetings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

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NEUROTRANSMITTER RELEASE THE NEUROMUSCULAR JUNCTION

Special Issue of Cell Biology International Reports Vol. 13, No. 12, December 1989

Page Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . iii Letter by Alex Mauro . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .._................ vi

Molecular Characterizations of synaptic vesicles M. Matteoli, F. Navone, C. Haimann, P. L. Cameron, M. Solimena and

P. De Camilli. Secretory organelles of neurons and their relationship to organelles of other cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 981

H. Zimmermann, W. Volknandt, A. Henkel, F. Bonzelius, A. Janetzko and T. Kanaseki. The synaptic vesicle membrane: origin, axonal distribution, protein components, exocytosis and recycling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 993

F. Benfenati, F. Valtotta, M. Bahler and P. Greengard. Synapsin I, a neuron-specific phosphoprotein interacting with small synap- tic vesicles and F-actin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1007

F. Valtorta, F. T. Tarelli, L. Campanati, A. Villa and P. Greengard. Synaptophysin and synapsin I as tools for the study of the exo-endocytotic cycle ,......................,........................................., 1023

V. P. Whittaker. Vasoactive intestinal popypeptide (VIP) as a cholinergic co-transmitter: some recent results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1039

xii

Acetycholine Release W. P. Hurlbut. The correlation between vesicle loss and quanta1

secretion at the frog neuromuscular junction . . . . . . . . . . . . . . . . . . . . . . . . . . . J. Heuser. The role of coated vesicles in recycling of synaptic vesicle

membrane . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . P. C. Molenaar. Acetycholine compartments in skeletal muscle . . . . . . . . . . . . . .

F. Grohovaz, R. Fesce and C. Haimann. Dual effect of potassium on transmitter exocytosis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

M. Israel, B. Lesbats and A. Suzuki. Characterization of a polyclonal antiserum raised against mediatophore, a protein that trans- locates acetycholine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

M. Raiteri, M. Marchi, G. Maura and G. Bonanno. Presynaptic regu- lation of acetylcholine release in the CNS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

F. Di Gregorio, R. Fesce, S. Cereser, G. Favaro and M. G. Fiori. Spon- taneous and nerve-evoked quanta1 transmission in regener- ated motor terminals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Ion Channels and Signal Transduction A. Mallart. The genetics of type A potassium channels in Drosophila

melanogaster . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ,........ C. Haimann and C. R. Bader. Sodium-activated potassium channel in

avian sensory neurons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . H. M. Gerschenfeld, D. Paupardin-Tritsch, C. Hammond and R. Harris-

Warrick. Intracellular mechanism of neurotransmitter-induced modulations of voltage-dependent Ca current in snail neu- rons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

E. Carbone, F. Clementi, A. Formenti, A. Poll0 and E. Sher. Action of Ca2+ agonists/antagonists in mammalian peripheral neurons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

E. Wanke, A. Becchetti, L. Bertollini and A. Feroni. Cross-talk between receptors coupled to calcium currents in adult but not neo- natal rat sensory neurons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

M. Parenti, G. Ceresoli, S. Consolo and A. Groppetti. Cross-talk between different intracellular signalling pathways in the rat hippocampus .,...........,,...,.............................................................

A. Volterra. Arachidonic acid metabolites as mediators of synaptic modulation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

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