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Correction: Myocyte-Specific Enhancer Factor 2 Acts Cooperatively with a Muscle Activator Region to Regulate Drosophila Tropomyosin Gene Muscle Expression Source: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, No. 15 (Jul. 23, 1996), pp. 8152-8153 Published by: National Academy of Sciences Stable URL: http://www.jstor.org/stable/40180 . Accessed: 08/05/2014 13:20 Your use of the JSTOR archive indicates your acceptance of the Terms & Conditions of Use, available at . http://www.jstor.org/page/info/about/policies/terms.jsp . JSTOR is a not-for-profit service that helps scholars, researchers, and students discover, use, and build upon a wide range of content in a trusted digital archive. We use information technology and tools to increase productivity and facilitate new forms of scholarship. For more information about JSTOR, please contact [email protected]. . National Academy of Sciences is collaborating with JSTOR to digitize, preserve and extend access to Proceedings of the National Academy of Sciences of the United States of America. http://www.jstor.org This content downloaded from 169.229.32.137 on Thu, 8 May 2014 13:20:30 PM All use subject to JSTOR Terms and Conditions
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Page 1: Correction: Myocyte-Specific Enhancer Factor 2 Acts Cooperatively with a Muscle Activator Region to Regulate Drosophila Tropomyosin Gene Muscle Expression

Correction: Myocyte-Specific Enhancer Factor 2 Acts Cooperatively with a Muscle ActivatorRegion to Regulate Drosophila Tropomyosin Gene Muscle ExpressionSource: Proceedings of the National Academy of Sciences of the United States of America,Vol. 93, No. 15 (Jul. 23, 1996), pp. 8152-8153Published by: National Academy of SciencesStable URL: http://www.jstor.org/stable/40180 .

Accessed: 08/05/2014 13:20

Your use of the JSTOR archive indicates your acceptance of the Terms & Conditions of Use, available at .http://www.jstor.org/page/info/about/policies/terms.jsp

.JSTOR is a not-for-profit service that helps scholars, researchers, and students discover, use, and build upon a wide range ofcontent in a trusted digital archive. We use information technology and tools to increase productivity and facilitate new formsof scholarship. For more information about JSTOR, please contact [email protected].

.

National Academy of Sciences is collaborating with JSTOR to digitize, preserve and extend access toProceedings of the National Academy of Sciences of the United States of America.

http://www.jstor.org

This content downloaded from 169.229.32.137 on Thu, 8 May 2014 13:20:30 PMAll use subject to JSTOR Terms and Conditions

Page 2: Correction: Myocyte-Specific Enhancer Factor 2 Acts Cooperatively with a Muscle Activator Region to Regulate Drosophila Tropomyosin Gene Muscle Expression

8152 Corrections Proc. Natl. Acad. Sci. USA (1996)

Biochemistry. In the article "Myocyte-specific enhancer factor 2 acts cooperatively with a muscle activator region to regulate Drosophila tropomyocin gene muscle expression" by Meei-Hua Lin, Hanh T. Nguyen, Claire Dybala, and Robert V. Storti,

which appeared in number 10, May 14, 1996 of Proc. Natl. Acad. Sci. USA 93, 4623-4628, the quality of the reproduction of Figs. 3-5 was unsatisfactory. The figures and their legends are shown below.

FS

FIG. 3. Whole mount in situ hybridization analysis of embryonic body wall expression of MEF2 trans- gene constructs. The probe for de- tecting transgene expression was an antisense 13-galactosidase riboprobe. (A) 1B transgene; (B) MA trans- gene; (C) 1BAMEF2 transgene; (D) MEF2 oligonucleotide transgene; (E) 3BAMEF2 transgene; (F) MA+MEF2 transgene. All embryos are approximately stage 16 and are positioned with anterior to the left and dorsal up.

D 4-

F

H.f , q

FIG. 4. Larval body wall and visceral muscle expression of MEF2 transgene constructs. (A, C, E, G, and I) Three-hour ,B-galactosidase-stained larval body wall muscles. (B, D, F, H, and J) Three-hour ,3-galactosidase-stained larval visceral muscles. (A and B) 1B transgene; (C and D) MA transgene; (E and F) 1BAMEF2 transgene; (G and H) MEF2 oligonucleo- tide transgene; (I and J) MA+MEF2 transgene. L, lateral muscles; D, dorsal muscles; DM, dorsal midline; H, circular muscles of the hindgut; M, longitudinal muscles of the midgut; MT, unstained malpighian tubules.

This content downloaded from 169.229.32.137 on Thu, 8 May 2014 13:20:30 PMAll use subject to JSTOR Terms and Conditions

Page 3: Correction: Myocyte-Specific Enhancer Factor 2 Acts Cooperatively with a Muscle Activator Region to Regulate Drosophila Tropomyosin Gene Muscle Expression

Corrections Proc. Natl. Acad. Sci. USA (1996) 8153

FIG. 5. Whole mount in situ hybridization analysis of TmI expression in mef2 mutant embryos. Antisense TmI was used as a riboprobe. (A) Endogenous TmI expression in stage 16 wild-type embryo. (B) Stage 16 Df(2R)XJ/Df(2R)P520 transheterozygous embryo, showing reduced TmI expression in body wall muscles; arrow shows TmI expression in a residual fused muscle fiber. (C) Stage 16 Df(2R)XJ/lDf(2R)P520 transheterozygous embryo, showing visceral muscle staining. (D) Stage 16 Df(2R)XJ/Df(2R)P520 transheterozygous embryo, showing dorsal vessel (arrow) staining. All embryos are orientated with anterior to the left. InA and B embryos are lateral views, in C and D they are ventral and dorsal views, respectively.

This content downloaded from 169.229.32.137 on Thu, 8 May 2014 13:20:30 PMAll use subject to JSTOR Terms and Conditions


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