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UBE Covaris

96 oneTUBE-10 AFA Plate Single Vessel Library Preparation

Normalize Shear DNA with AFA

End Repair and Adapter

LigationClean-up Final

Library

• Highest Accuracy and Precision

Transfer steps eliminated with oneTUBE

• All in oneTUBE vessel

• Maximized Sample Recovery• Optional: AFA®

Enhanced Clean-up

oneTUBE NGS Workflow

The 96 oneTUBE-10 AFA Plate is a highly specialized polymer vessel designed for high-throughput library preparation applying the Covaris gold standard DNA shearing technology:

• Fully compatible with automated liquid handlers and robotic platforms

• Eliminates transfer steps, improves turn-around-time, and decreases cost of reagents & consumables

• Preserves sample complexity for comprehensive genome coverage

• Powered by AFA-energetics™

96 oneTUBE-10 AFA Plate 8 oneTUBE-10 AFA Strip

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UBE Covaris

Time(s) Average Size (bp) Stdev CV Number of

ReplicatesTarget

Size (bp)Target

(%)

45 352 11.6 3.3 24 350 101

220 175 3 1.7 24 175 100

Actual average size base pair (bp) compared to expected target size for 175 bp and 350 bp with the 96 oneTUBE-10 AFA plate. Data shown for a working volume of 10 µl.

The Standard in NGS for DNA FragmentationHighly accurate, reproducible, and robust sample preparation powered by

Adaptive Focused Acoustics® (AFA®) technology

USA: Covaris, Inc. • Tel: +1 781.932.3959 • Fax: +1 781.932.8705 • Email: customerservice@covaris.com | Europe: Covaris Ltd. • Tel: +44 (0)845 872 0100 • Fax: +44 (0)845 384 9160 • Email: emeacustomerservice@covaris.comWeb: www.covaris.com • Information: info@covaris.com • Applications: applicationsupport@covaris.com • Service and Support: techsupport@covaris.comPart Number: M020067 | Rev B | Edition March 2018 | INFORMATION SUBJECT TO CHANGE WITHOUT NOTICE | FOR RESEARCH USE ONLY | NOT FOR USE IN DIAGNOSTIC PROCEDURES | COPYRIGHT 2018 COVARIS, INC.

Part # Product Name

520249 96 oneTUBE-10 AFA Plate

520250 96 oneTUBE-10 AFA Plate (10 pack)

500588 96 oneTUBE-10 Rack

A.

B

B.

Mechanical DNA Shearing Performances

Figure 4 – Reproducible DNA Shearing 48 DNA replicates were treated in the first 6 rows of a 96 oneTUBE-10 AFA Plate (1 to 6) for a targeted size of 150 bp or 350 bp. 50 ng human genomic DNA (Promega PN G3041) was sheared on a Covaris LE220-plus with the following settings:

Targeted size 350 bp 150 bp

PIP (W) 250 250

DF (%) 20 20

CPB 50 50

Time (s) 52 360

DNA was analyzed with a Fragment Analyzer (High Sensitivity NGS Fragment Analysis Kit) and the Boxplot graph of the DNA electropherograms was generate by a home-written MATLAB R2016b script.

A. Overlay of the 48 technical replicates after amplitude normalization: 150 bp (Blue) or 350 bp (Red). B. Box plot of the 48 replicates for each targeted size (350 bp left, 140 bp right), organized by row (1 to 6), and

then position (A to H). Red line is the median, blue dot the mean and green diamond the mode. Each box represents 25th to 75th region

A:

----------------- oneTUBE-10 350 bp----------------- oneTUBE-10 150 bp

FIgure A. DNA was analyzed with a Fragment Analyzer (High Sensitivity NGS Frag-ment Analysis Kit). Overlay of the 48 technical replicates after amplitude normalization: 150 bp (Blue) or 350 bp (Red).

Figure B. The Boxplot graph of the DNA electropherograms was generated by a custom MATLAB R2016b script.

Box plot of the 48 replicates for each targeted size (350 bp left, 150 bp right), organized by row (1 to 6), and then position

Red line is the median, blue dot the mean and green diamond the mode. Each box represents 25th to 75th region.