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ASSIGNMENT CYTOLOGY

AT 2700

NORMAL CEELS OF SQUAMOUS EPITHELIUM

NAME :NORHASIMAH BINTI ABDUL LATIF

:NOOR SYAZWANI BINTI ABD WAHID

ID NUMBER :111072-05803-010

:111072-05807-010

NAME LECTURER :MADAM SELAMAH BITI MANSOR

DATE SUBMISSION: 30 MARCH 2012

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ther Images from this Article

Basaloid squamous cell carcinoma. Note the peripheral palisading of the primitive high-gradetumor cells within the invasive tumor nests and a lack of stromal response (H.E. 100)

ASC with nuclear enlargement and mild hyperchromasia (ThinPrep. Papanicolaou, 400)

ASC with bi/multinucleation. Intermediate squamous cells with multinucleation and nuclear

enlargement but normochromasia and smooth nuclear membranes (SurePath, Papanicolaou,

400)

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LSIL (koilocytes). Several intermediate squamous cells with cytoplasmic perinuclear halo

and nuclear abnormalities (ThinPrep. Papanicolaou, 400)

LSIL with parakerat

Other Images from this Article

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Basaloid squamous cell carcinoma. Note the peripheral palisading of the primitive high-grade

tumor cells within the invasive tumor nests and a lack of stromal response (H.E. 100)

ASC with nuclear enlargement and mild hyperchromasia (ThinPrep. Papanicolaou, 400)

ASC with bi/multinucleation. Intermediate squamous cells with multinucleation and nuclear

enlargement but normochromasia and smooth nuclear membranes (SurePath, Papanicolaou,400)

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ASC, cannot rule out a HSIL. A small group of metaplastic cells showing nuclear

enlargement and hyperchromasia. High N:C ratio (>75%) also noted (SurePath,

Papanicolaou, 400)

Atypical repair. Loosely cohesive squamous cells with enlarged nuclei and prominent

nucleoli. Considerable degree of anisonucleosis is noted. However, there is no single atypicalcells (SurePath, Papanicolaou, 400)

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LSIL. Two intermediate squamous cells with nuclear enlargement (>3), hyperchromasia,

and coarse chromatin. One cell also demonstrates binucleation (SurePath, Papanicolaou,400)

LSIL (koilocytes). Several intermediate squamous cells with cytoplasmic perinuclear halo

and nuclear abnormalities (ThinPrep. Papanicolaou, 400)

LSIL with parakeratotic cells in thick cluster with variable degree of nuclear atypia. The cells

are arranged in a disordered fashion (SurePath, Papanicolaou, 400)

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Glycogen accumulation resulting in cytoplasmic halo, which occupies the entire cells and

displays pale yellow tint. No nuclear abnormalities are noted (SurePath, Papanicolaou, 400)

HSIL. Sheet of metaplastic squamous cells with nuclear enlargement, hyperchromasia,

increased N:C ratio, and irregular nuclear contours (SurePath, Papanicolaou, 400)

HSIL. Two crowded groups of squamous cells with high N:C ratio and hyperchromatic nuclei(ThinPrep. Papanicolaou, 400)

HSIL. Dysplastic keratinized cells with dense, orangeophilic cytoplasm; increased N:C ratio;

hyperchromatic nuclei; and irregular nuclear membrane (SurePath, Papanicolaou, 400)

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LSIL cannot rule out HSIL. Atypical squamous cells with increased N:C ratio (>50%) and

less mature cytoplasm (SurePath, Papanicolaou, 400)

HSIL with glandular involvement. A crowded group with flattening of the nuclei at one edge

resulting in a smooth border. Individual cells demonstrate increased N:C ratio andhyperchromatic nuclei (SurePath, Papanicolaou, 400)

Keratinizing SCC. Bizarre keratinized squamous with pleomorphic, hyperchromatic nuclei

and moderate amount of orangeophilic cytoplasm (SurePath, Papanicolaou, 400)

Keratinizing SCC. A small group of dyskaryotic squamous cells with prominent nucleoli in a

hemorrhagic background (conventional preparation, Papanicolaou, 400)

Nonkeratinizing SCC. Syncytial aggregate of squamous cells with marked increased in N:C

ratio and hyperchromatic nuclei. Distinct nucleoli are noted in scattered cells. The cytoplasm

is scant and cyanophilic with indistinct cell borders (SurePath, Papanicolaou, 400)

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Nonkeratinizing SCC. Dyscohesive malignant squamous cells admixed with amorphous

debris and inflammatory cells (conventional preparation, Papanicolaou, 400)

Tumor diathesis consisting of necrotic debris and inflammatory cells tend to be clumped in

liquid-based preparation. A nearby cluster of neoplastic squamous cells (ThinPrep.Papanicolaou, 200)

Lactobacilli with cytolysis. Numerous bacterial rods are noted in the background. There are

also several naked nuclei devoid of cytoplasm (SurePath, Papanicolaou, 400)

Clue cells. Superficial squamous cells are covered diffusely by bacterial cocci. Note that the

background is usually clean in liquid-based preparation (ThinPrep. Papanicolaou, 400)

Actinomyces. Colony of filamentous bacteria, resembling a cotton ball (SurePath,

Papanicolaou, 400)

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Atrophy. Large fragments of parabasal cells presented as hyperchromatic crowded group.

Preservation of nuclear polarity helps in the differential diagnosis (SurePath, Papanicolaou,

400)

Hyperkeratosis. Clusters of anucleated squames devoid of any nuclei (ThinPrep,Papanicolaou, 400)

Parakeratosis. Strips of small superficial squamous cells with intense opaque orangeophilic

cytoplasm (SurePath, Papanicolaou, 400)

Parakeratosis. Parakeratotic squamous cells arranged in a concentric circle, resulting in a

squamous pearl (SurePath, Papanicolaou, 400)

Atypical parakeratosis. Parakeratotic squamous cells with nuclear enlargement and irregular

nuclear contours. This should be classified as ASC under the current TBS (SurePath,

Papanicolaou, 400)

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Reactive cellular changes. Metaplastic squamous cells showing mild nuclear enlargement and

small perinuclear cytoplasmic halos with poorly defined borders. A reactive intermediate

squamous cell demonstrating binucleation (SurePath, Papanicolaou, 400)

Repair. Flat, monolayer, cohesive sheet of squamous cells with normal N:C ratio, nuclearenlargement, vesicular chromatin, and prominent nucleoli (SurePath, Papanicolaou, 400)

Therapeutic changes. Very large and bizarre squamous cells with nuclear enlargement.

However, the N:C ratio remains low and the nuclear chromatin appears smudged

(conventional preparation, Papanicolaou, 400)

Cervical flat condyloma. Note the cytoplasmic vacuolation around the nuclei with

condensation of the cytoplasm at the periphery, nuclear atypicality, and multinucleation (H.E.

200)

Cervical intraepithelial neoplasia 1 (CIN 1). Note the presence of atypical neoplastic

proliferation limited to the lower third of the squamous epithelium (H.E. 200)

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Cervical intraepithelial neoplasia 2 (CIN 2) (H.E. 200)

Cervical intraepithelial neoplasia 3 (CIN 3) (H.E. 100)

Squamous metaplasia at the cervical transformation zone. Note the absence of nuclear atypia

(H.E. 100)

Transitional cell metaplasia. Note the streaming squamoid cells with nuclear grooves and the

absence of high-grade nuclear atypia (H.E. 200)

Naked germinal center. Note the presence of highly active lymphoid cells of germinal center

and absence of the mantle zone lymphocytes in this extreme example (H.E. 200)

Microinvasive squamous cell carcinoma. Note the abrupt keratinization (paradoxical

maturation) and the presence of stromal response (stromal edema, desmoplasia, andprominent inflammatory changes) (H.E. 40)

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Squamous cell carcinoma, large cell and keratinizing (H.E. 40)

Squamous cell carcinoma, large cell and nonkeratinizing (H.E. 100)

License

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