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Cryopette

demonstration

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Cryopette is now CE marked and FDA cleared!

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Cryopette

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Cryopette product specifications

Straw: Polycarbonate plastic (inner diameter: 275m)

Bulb: Silicone

Max volume in straw: 1.2l

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Heat sealer

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UltraSeal 21 ultrasonic sealer

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Cutting block / razor

Razor-like item must be used to open the Cryopetteto avoidsqueezing the opening

Any sharp razor, scalpel, surgical scissor or other sharp cuttingdevice will work fine..

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CryoBath with rack

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Alternative goblets and cryocanes

www.minitube.de

If customers has storage facilities that do NOT fit our 13mm gobletsand canes or the above shown alternatives please get as much infoas possible and we will assist in finding a solution

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Labelling the Cryopette

System from Brady recommended

Wrap label with patient informationaround the bulb

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Cryopetteprotocol - Cooling

1. Prepare Blastocyst(s) in preferredvitrification cooling media.

2. Label Cryopettewith patient informationand attach to the Displacement Bulb.

3. Squeeze bulb and gently loadBlastocyst(s) in an unbroken columnof media.

4. Confirm that Blastocyst(s)are located correctly

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Cryopetteprotocol Cooling (cont)

5. Seal open end of Cryopette

6. Confirm secure seal by depressing Bulb

under microscope while looking for any leaks.

7. Plunge Cryopette into the liquid nitrogen

reservoir.Store the sealed Cryopette into goblet on cryocane.

Once experienced, whole procedure takes

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Cryopetteprotocol - Warming

1. Remove Cryopettefrom liquidnitrogen and completely submerge into37C water for 5 seconds.

Gently wipe off excess water.

2. Open Cryopettewith sharp razorjust above the heat seal at SealingMark.

3. Gently depress Displacement Bulband expel Blastocyst(s) into preferredvitrification warming media.

37C

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Clinical data

Mouse (127) and abnormal human (38)blastocysts vitrified with CryopetteandCryoloop (open system)

24-48hr post-warming blastocysts wereexamined for re-expansion

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Mouse blastocysts vitrified with Cryopette

(n=73) and Cryotip (n=70) Recovery and survival were evaluated

following warming

RecoveredNot

recoveredSurvival

(recovered cells) Not survived

Cryopette

(n=73)73

(100%)70

(96%)3

(4%)

Cryoptip(n=70)

57(81%)

13(19%)

48(84%)

9(16%)

Total: 143 130 13 118 12

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MII Oocytes No %

Warmed 53

Recovered 53 100

Survived 49 92.4

Preliminary data* document great recovery

Visual inspection possible (correct position of cells and quality of seal)

Controlled transfer under visual inspection (with other devices cells arewashed off the carrier into the warming medium)

*Dal Canto, presented at ORIGIO booth at ASRM 2010unpublished data using protocol described in Fadini et al. 2009, RBM Online.

*Dal Canto, unpublished data usingprotocol described in Fadini et al. 2009,RBM Online.

Blastocysts; n=40(25 patients)

Closed system(Cryopette)

Recovery (%) 100

Survival (%) 88.9

Clinical PR/transfer (%) 33.3

Implantation rate (%) 25

D2/D3 embryos; n=69(37 patients)

Closed system(Cryopette)

Recovery (%) 100

Survival (%) 98.5

Clinical PR/transfer (%) 27.3

Implantation rate (%) 17.6

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Medicult vitrification cooling protocol

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Medicult vitrification warming protocol

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Cooling rates - open vs closed vitrification devices

Camus et al, 2006

Closed

OpenCryopette