CRYOPRESERVATION OF CARDIOMYOCYTES DERIVED FROM INDUCED PLURIPOTENT STEM CELLS CONFIDENTIAL Cláudia Correia 1,2 , Margarida Serra 1,2 , Marcos FQ Sousa 1,2 , Catarina Brito 1,2 , Karsten Burkert 3 , Azra Fatima 3 , Juergen Hescheler 3 , Manuel JT Carrondo 2,4 , Tomo Saric 3 , Paula M. Alves 1,2 1 Instituto de Tecnologia Química e Biológica (ITQB) – Universidade Nova de Lisboa (UNL), Oeiras, Portugal; 2 Instituto de Biologia Experimental e Tecnológica (IBET), Oeiras, Portugal; 3 Institute for Neurophysiology University of Cologne, Cologne, Germany 4 Faculdade de Ciências e Tecnologia (FCT), Universidade Nova de Lisboa (UNL), Monte de Caparica, Portugal (http://tca.itqb.unl.pt; www.ibet.pt) Presented at “Scale-up and Manufacturing of Cell-based Therapies II” 21st-23rd January 2013 San Diego, California, USA
Transcript
CRYOPRESERVATION OF CARDIOMYOCYTES DERIVED FROM
INDUCED PLURIPOTENT STEM CELLS
CONFIDENTIAL
Cláudia Correia1,2, Margarida Serra1,2, Marcos FQ Sousa1,2, Catarina Brito1,2, Karsten Burkert3, Azra Fatima3, Juergen Hescheler3, Manuel JT Carrondo2,4, Tomo Saric3, Paula M. Alves1,2
1 Instituto de Tecnologia Química e Biológica (ITQB) – Universidade Nova de Lisboa (UNL), Oeiras, Portugal; 2 Instituto de Biologia Experimental e Tecnológica (IBET), Oeiras, Portugal; 3 Institute for Neurophysiology University of Cologne, Cologne, Germany 4 Faculdade de
Ciências e Tecnologia (FCT), Universidade Nova de Lisboa (UNL), Monte de Caparica, Portugal
(http://tca.itqb.unl.pt; www.ibet.pt)
Presented at “Scale-up and Manufacturing of Cell-based Therapies II” 21st-23rd January 2013
San Diego, California, USA
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CRYOPRESERVATION OF CARDIOMYOCYTES
CELL VIABILITY (FDA – VIABLE CELLS, PI – DEAD CELLS)
Cryopreservation Medium Formulation: FBS + 10% DMSO vs. CryoStorTM CS10
Immediately after thawing 1 day after thawing 3 days after thawing
CryoStorTM CS10
FBS + 10% DMSO
Before cryopreservation
200 m
200 m
RECOVERY OF TOTAL CELLS (metabolic activity - AlamarBlue assay)
0
20
40
60
80
100
120
Before cryopreservation
1 day after thawing
3 days after thawing
%R
eco
very
FBS+10%DMSO
CryostorTM10
CRYOSTORTM CS10 Higher cell viability and cardiomyocyte recovery after thawing
CryoStoreTMCS10 improves cell survival by reduction of both apoptosis and necrosis by inhibition of cellular stress during cryopreservation (Malpique et al., Tissue Eng Part C, 2010)
CONFIDENTIAL
Before cryopreservation
Immediately after thawing 1 day after thawing 3 days after thawing αMHC-GFP+ cells
CR
YO
STO
RTM
C
S10
FB
S +
10
%
DM
SO
200 m
200 m
CRYOPRESERVATION OF CARDIOMYOCYTES Cryopreservation Medium Formulation: FBS + 10% DMSO vs. CryoStorTM CS10
3
CRYOSTORTM CS10 Higher cell viability and cardiomyocyte recovery after thawing
CryoStoreTMCS10 improves cell survival by reduction of both apoptosis and necrosis by inhibition of cellular stress during cryopreservation (Malpique et al., Tissue Eng Part C, 2010)
