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ThisTestBookletwillcontain145(20PartA+50PartB+75PartC)MultipleChoiceQuestions(MCQs).Candidateswillberequiredtoanswer15 inpartA,35inPartBand25questions inPartCrespectively(No.ofquestionstoattemptmayvaryfromexamtoexam).Incaseanycandidateanswersmorethan15,35and25questionsinPartA,BandCrespectivelyonlyfirst15,35and25questionsinPartsA,BandCrespectivelywillbeevaluated.Questions inPartsAandBcarrytwomarkseachandPartCquestionscarryfourmarkseach.Therewillbenegativemarking@25%foreachwronganswer.Beloweachquestion,fouralternativesorresponsesaregiven.OnlyoneofthesealternativesistheCORRECTanswertothequestion.

PARTA

1.Profitofafirmgrowsatarateof15%peryearforthefirstthree consecutive years. For the next three years, the profitlevel remains stagnant. From the 6th year till the 9th year, itagaingrowsatarateof15%peryear.Whichofthefollowinggraphsdepictsthesefacts?(Answer1)

2. A pond is deepest at its centre and becomes shallowuniformlytowardstheedge.IfthedepthofwateratthecentreinMay is half its value in August, thewater contained in thepond(Answer3)(1)inMayisgreaterthanhalfthatinAugust(2)inAugustisequaltotwicethatinMay(3)inMayislessthanhalfthatinAugust(4)inAugustislessthantwicethatinMay3.Theseries representing thesumof theareasof theshadedequilateraltrianglesinthefigurebelowis(Answer3)

4.Whichofthefollowingvitaminswillnotbesynthesizedinapersonconfinedtoadarkcellforalongtime?(Answer4)1.A 2.B3.C 4.D

5.Floweringissomeplantsisstronglyinfluencedbythephotoperiod. A farmerwas growing two species of plants, A and Bnearaseacoastwherealighthousewaslocated.Heobservedthat species A flowered profusely while species B did not.Whichofthefollowingiscorrect?(Answer1)1.SpeciesArequireslongdurationofdaywhilespeciesBneedsashorterday2. Species B requires longer duration of day while species Aneedsashorterduration3.Bothspeciesrequireshortdurationofday4.Bothspeciesrequirelongdurationofday6. Pneumatophores are modified roots in some plants likeRhizophora growing in swampy areas that come out of thegroundandgrowverticallyupwards.Themainfunctionofsuchrootsisto(Answer1)(1)helpobtainoxygenforrespiration(2)providesupport(3)adsorbandconductwaterandminerals(4)storefood7. A cube of side 1 cm is painted by putting a lacquer ofthickness , negligible compared to the side of the cube. Thevolumeofthepaintedcubeisapproximately(1)1+cm3 (2)1+3cm3(3)1+33cm3 (4)1+3cm38. A candle is burning inside a sealed glass jar. The pressureand temperature of the air within the jar are plotted as afunctionoftime.Whichofthefollowinggraphsrepresentsthisprocesscorrectly?(Answer1)

9.Theresultoftaking1scomplementofthesumofthebinarynumbers110and101willbe(Answer1)(1)1011 (2)0011(3)0100 (4)011010. Which of the following straight lines passes through thepoint(1,1)?(1)y=2x+3 (2)2y=x6(3)x=1 (4)x=y+111.Whichofthefollowing1molar(aqueous)solutionhasthehighestnumberdensityofions?(Answer2)(1)Glucose (2)CaCl2(3)NaNO3 (4)KCl12.Howmanytwodigitevennumberscanbecomposedfromninedigits1,2,39?(Answer3)(1)50 (2)81(3)45 (4)36



13. Complete combustion of cyclohexane (C6H12) isrepresentedbytheequation

C6H12+xO2yCO2+zH2OThevaluesofx,yandz,respectively,are(Answer1)(1)9,6,6 (2)10,6,4(3)6,12,10 (4)4,8,1214. How many distinct trichlorobenzenes (C6H3Cl3) shouldexist, given that benzene (C6H6) has a regular hexagonalgeometry?(Answer4)(1)6 (2)1(3)2 (4)315.MercuryisclosertotheSunthanVenus.YetVenusishotterbecauseithas(Answer1)(1)adominantCO2atmosphere(2)adominantmethaneatmosphere(3)sulphuricacidclouds(4)anatmospheredevoidofoxygen16. In a simple pendulum experiment, a student records thefollowingreadings. If thetrueperiodof thependulumis10s,then thepercent error is the largest for theobservationwithserialnumber(Answer2)

17. A container holding normal air (1 bar pressure, roomtemperature) is being evacuated. The normal composition ofair is approximately 78%N2, 21%O2, 0.9% Ar and traces ofCO2(0.04%)andwatervapour(0.02%).After thepressure inthecontainerfallstoabout103mbar,therelativefractionsofthecomponentswillbe(Answer2)1.N2andO2approximatelyequalandgreaterthanH2O2.N2,O2,ArapproximatelyequalandgreaterthanH2O3.N2,O2,Arintheoriginalproportion,butN2lessthanH2O4.N2,O2,Arintheoriginalproportion,andN2greaterthanH2O18. An endoscope is a device for observing internal organs,usingacombinationofa lampandanoptical fibre.Theimageseenisdueto(Answer1)(1)lightreflectedbytheorganandtransmittedbyinternalreflectionthroughthefibre(2) light refractedby theorganand transmittedbyrefractionthroughthefibre.(3) light refracted by the organ and transmitted by internalreflectionthroughthefibre.(4) light emitted by the organ and transmitted by refractionthroughthefibre.19.Howmanytimesinadayistheanglebetweentheminuteandhourhandsofaclockequal toanangle,where00



What is the probable order of these genes on the bacterialchromosome?(Answer3)1.thr,leu,pro,his2.pro,leu,thrandthepositionofhiscannotbedetermined.3.thr,leu,pro,andthepositionofhiscannotbedetermined4.his,pro,leu,thr31. Two varieties of maize averaging 48 and 72 inches inheight, respectively, are crossed. The F1 progeny is quiteuniformaveraging60inchesinheight.Ofthe500F2plants,theshortest2are48inchesandthetallest2are72inches.Whatisthe probable number of polygenes involved in this trait?(Answer1)1.Four. 2.Eight.3.Sixteen. 4.Thirtytwo.32.RepairofdoublestrandbreaksmadeduringmeiosisintheyeastSaccharomycescerevisiae(Answer3)1.occursmostlybynonhomologousendjoining.2.occursmostlyusingthesisterchromatidasatemplate.3.occursmostlyusing thehomologous chromosomeasatemplate4.isassociatedwithahighfrequencyofmutations.33.Whichof the followingsignalingmoleculesenters thecelltoinitiateitsaction?(Answer4)1.Transferrin 2.Insulin3.Glucagon 4.Thyroxine34.Themodeofactionoftheanticancerdrugmethotrexateisthroughitsstrongcompetitiveinhibitionon(Answer1)1.dihydrofolatereductase2.thymidinesynthase.3.thymidinekinase. 4.adenylatecyclase.35.Whichclassof immunoglobulinswill increase incaseofachronicinfection?(Answer2)1.IgA 2.IgG3.IgM 4.IgE36.Whenprospectiveneuroectodermfromanearlyamphibiangastrulaistransplantedintheprospectiveepidermalregionofa recipient (earlygastrula)embryo, thedonor tissuewillgiveriseto(Answer2)1.neuraltube. 2.epidermis.3.neuraltubeandnotochord. 4.neuraltubeandepidermis.37.Amphibianoocytesremainforyearsinthediplotenestageof meiotic prophase. Resumption of meiosis is initiated by(Answer4)1.gonodatropichormone. 2.growthhormone.3.oestrogen. 4.progesterone.38.Agroupofsixcellscalled'equivalencegroupcells'dividetoform the vulval structure in Caenorhabditis elegans. They arecalledsobecause(Answer2)1.theyhavesimilarfatesduringdevelopmentofvulva.2. all the six cells are competent to form vulva and canreplaceeachotherundervariousexperimentalconditions.3. they are all under the influence of the anchor cell, signalsfromwhichinitiatevulvaldevelopment.4.theyinteractwitheachothertoformthevulvalstructure.39.Due to thepresenceof cellulose in the cellwallofplants,leafshapeisdeterminedintheleafprimoridaby(Answer2)1.ratesofcelldivision. 2.planesofcelldivision.3.cellmigration. 4.cellcellinteractions.40. DCMU inhibits electron transport in chloroplast bypreventingthereductionof(Answer4)1.P680. 2.QA.3.PQ. 4.QB.

41. In higher plant leaves, the reduction of nitrate toammonium takes place by the combined action of nitratereductaselocalizedincytosolandnitritereductaselocalizedin(Answer3)1.peroxisomes. 2.mitochondria.3.chloroplasts. 4.cytosol.42. In higher plants, the red/farred sensory photoreceptor,phytochrome, is a lightregulated kinase. Which of thefollowingclassesofkinasesdoesitrepresent?(Answer2)1.Twocomponentsensorregulator(histidinekinase).2.Twocomponentsensorregulator(serine/threoninekinase).3.Leucinerichrepeat(LRR)receptorkinase.4.Calciumdependentproteinkinase.43. Vesiculararbuscular mycorrhiza (VAM) represents abeneficial association between plant roots and fungus,wherefungusassistsplantsinobtainingfromthesoil(Answer4)1.iron. 2.zinc.3.sulphate. 4.phosphate.44.Unidirectional propagation of electrical signal in nervoussystemis(Answer2)1.proportionaltothelengthofaxon.2.duetochemicalsynapse.3.duetoelectricalsynapse.4.proportionaltomyelination.45. A myasthenia gravis patient develops muscle paralysisbecause(Answer3)1. the nerve terminal at the neuromuscular junction fails toreleaseacetylcholine.2. although enough acetylcholine is released at theneuromuscularjunction,itisdestroyedbyacetylcholinesterase.3.thepatientdevelopsimmunityagainsthisownacetylcholinereceptor.4.thepatientdevelopsantibodyagainsthisownacetylcholine.46. Inhibin from sertoli cells of testes selectively inhibits(Answer2)1.luteinizinghormone.2.folliclestimulatinghormone.3.thyroidstimulatinghormone.4.growthhormone.47.Hawk'sretinapossessesalargenumberof(Answer3)1.rods. 2.melanocytes.3.cones. 4.kuffercells.48. E. coli cells were grown in N15 medium for severalgenerations and then shifted to normal medium for onegeneration. If the DNA isolated from the culture would becentrifuged on a CsCl equilibriumdensity gradient, the resultwillbe(Answer1)1. a singlebandofdoublehelixDNAconsistingofone strandwithN14andanotherwithN15label.2.singlebandofdoublehelixDNAconsistingofN14andN15inboththestrands.3.twobandscontainingdoublehelixDNAeachcontainingbothN14andN15label.4.twobandscontainingsinglestrandedDNAonewithN14andotherwithN15label.49.Whichofthefollowingprocessesdoesnottakeplaceinthe53direction?(Answer4)1.DNAreplication 2.Transcription3.Nicktranslation 4.RNAediting



50.Adeletionof threeconsecutivebasesinthecodingregionofagenecannotresultin(Answer4)1.deletionofasingleaminoacidwithoutanyotherchange intheprotein.2.replacementof twoadjacentaminoacidsbyasingleaminoacid.3. replacementofa singleaminoacidbyanotherwithoutanyotherchangeinsequenceoftheprotein.4.productionofatruncatedprotein.51. Deletion of the leader sequence of trp operon of E. coliwouldresultin(Answer2)1.decreasedtranscriptionoftrpoperon.2.increasedtranscriptionoftrpoperon.3.noeffectontranscription.4. decreased transcription of trp operon in the presence oftryptophan.52. In the endodermis of higher plants, the role of Casperianstripistocontrolthewatermovementsothatitflows(Answer4)1.betweenthecells.2.throughtheplasmamembrane.3.throughthecellwall.4.throughthetransfusiontissue.53.ThereptilianorderSquamataincludes(Answer4)1.crocodilesandalligators. 2.thelivingfossil'tuatara'.3.turtlesandtortoises. 4.snakesandlizards.54.Cultivatedbananasaresterilebecause(Answer3)1.maleflowerbearingplantsareveryrare.2.theylacknaturalpollinatorsinthecropplants.3.theyaretriploidandthereforeseedsarenotset.4.theyareacrossoftwounrelatedspecies.55.Onelifehistorytraitthatisnotcharacteristicofverysmallsizedorganismsis(Answer1)1.delayedageatfirstreproduction.2.earlierageatfirstreproduction.3.highpopulationgrowthrate.4.shortlifespan.56.Whichofthefollowingstatementsisthemostappropriateexampleofcharacterdisplacement?(Answer2)1. Two related species depending on the same prey speciesavoidcompetitionbyfeedingatdifferenttimesoftheday.2.Thebodysizesoftworelatedspeciesareverysimilarwhentheyareallopatric,butingeographicalareasofsympatry,onespeciesissignificantlysmallerthantheother.3.Thefoodnicheofaspeciesisgenerallywiderintheabsenceofcompetingspeciesthanintheirpresence.4. Closely related species can coexist if their densities areregulatedbyapredator.57. In a population growing logistically and approachingCarryingCapacity(K), thechangeindensity(N)perunittime(dN/dt)ismaximumwhenNequalsto(Answer2)1.K2 2.K/2.3.K. 4.K/458.ThelossesofozoneoverArcticaresignificantlylowerthanthatoverAntarcticabecause(Answer4)1.polarvortexoverArcticisnotastightasoverAntarctic.2.Arcticstratospherewarmsslowerinthespring.3. concentration of chlorine in the atmosphere over Arctic islessthanoverAntarctic.4. freezing of NO2 and CH4 are slower over Arctic than overAntarctic.

59. Which of the following species replacement sequencedepictstolerancemodelofcommunitysuccession?(Answer2)

60.Evolutionarily,withwhichof the followingcouldparentalcareinanimalsbeassociated?(Answer1)1.Smallerclutchsize. 2.Polygamy.3.Greaterlongevity. 4.Semelparity.61.

Thechangeinatraitwithtimeasaresultofnaturalselectionisshownabove.Thistypeofnaturalselectionis(Answer3)1.directional. 2.disruptive.3.stabilizing. 4.random.62. During which geological period did the greatestdiversificationoflifeonearthoccur?(Answer3)1.Permian 2.Jurassic3.Cambrian 4.Ordovician63.Which of the following plant groups evolved during theSilurianperiod?(Answer2)1.Bryophyta 2.Psilophyta3.Lycophyta 4.Spherrophyta64. Which of the following GM crops is the most widelycultivatedglobally?(Answer1)1.Herbicideresistantsoybean. 2.Insectresistantcotton.3.Insectresistantbrinjal. 4.delayedripeningtomato65. PCR based DNA amplification is an essential feature ofwhich of the following combination of molecular markers?(Answer2)1.RFLP,AFLPandSSR. 2.AFLP,SSRandRAPD.3.RFLP,RAPDandSSR. 4.RAPD,RFLPandSSR.66. The genes whose promoters are extensively used forproduction of pharmaceutical proteins in transgenic dairycattlesare(Answer2)1.lactalbuminandovalbumin.2.lactoglobulinandcasein.3.lactoferrinandtransferrin. 4.caseinandovalbumin.67. A biochemist purifies a new enzyme, generating thefollowingpurificationtable.(Answer2)S.No.

Procedure Totalprotein(mg)

Activity(units)

i.ii.iii.iv.v.

CrudeExtractSaltprecipitationIonexchangechromatographyAffinitychromatographySizeexclusionchromatography

20,0005,0002005045

4,000,0003,000,000800,000750,000675,000

Themosteffectivepurificationstepis1.iv. 2.iii.3.v. 4.ii.



68. Similarities in sequence and function of two proteinsindicate that they are members of a family that share acommonancestor. If they are fromdifferent species, they arecalled(Answer2)1.homologs. 2.orthologs.3.paralogs. 4.proteologs. 69. The conformation of a 30residue peptide is studied byNMRspectroscopy.The JNH formostof theamideprotons is4Hz. The 2D NOESY spectrum shows prominent NiNi+1connectivities.Theconformationofthepeptideis(Answer3)1.antiparallelsheet. 2.parallelsheet.3.helixlike. 4.unordered.70.Asampleof32Pdisintegratesatarateof30,120dpm.Theradioactivity inmicrocuries (1 curie=3.7x1010dps) is (Answer3)1.15,060. 2.1.36x102.3.81.6x102. 4.1.36x108.

PARTC71. A protein polypeptide chain exists in helicalconformationinasolventandithasavalueof30,000degcm2dmol1forthemeanresidueellipiticityat222nm([]222)inthetemperaturerange20500C.Onraisingthetemperatureabove500C, []222 increases and reaches a value of 2,000 deg cm2dmol1 at 700C and the value of []222 remains unchangedabove 700C. The observed value of []222 is 14,000 deg cm2dmol1 at 600C. If one assumes that the heatinduceddenaturation is a twostateprocess, the fractionofhelix at600Cis(Answer3)1.0.40 2.0.433.0.50 4.0.5772.100ml of 0.02M acetic acid (pKa= 4.76) is titratedwith0.02NKOH.After adding some base to the acid solution, theobserved pH is 2.76. At this pH degree of protonation is(Answer4)1.0%. 2.10%.3.90%. 4.99%.73.There are hydrogen bond donors (D), and acceptors (A).Whenthemixture(A+D)istransferredfromwater,designatedas(w)toanonpolarsolvent(np),thefreeenergychange(G)of transfer is 6.12 kcal mol1 for the (A+D)mixture. A and DformahydrogenbondinthisnonpolarsolventandGforthisprocess is 2.4 kcal mol1. When the hydrogen bondedmoleculeADistransferredfromwatertothenonpolarsolventG for thisprocess is0.62kcalmol1.ThevalueofG for theformation of hydrogen bond (A+B AB) in water is(Answer3)1.0.1kcalmol1. 2.+3.1kcalmol1.3.3.1kcalmol1. 4.1.78kcalmol1.74.Inahumancell, theconcentrationofATP,ADPandPiare2.25, 0.50 and 0.825mM respectively. The free energy ofhydrolysisofATPatpH7.0and25Cis(Answer4)1.30.5kJ/mol. 2.61kJ/mol.3.15.25kJ/mol. 4.52kJ/mol.75.Which one of the following enzyme reaction representsnoncompetitiveinitiation?(Answer4)

76. The sequence ofmonosaccharides including position andconfiguration of glycosidic bonds in a glycoprotein is to bedetermined. Which one of the following methods can beemployed?(Answer3)1. Glycoprotein removal of oligosaccharides by alkalinehydrolysis nuclear magnetic resonance analysis of cleavedmixtureofoligosaccharides2.Twodimensionalnuclearmagneticresonancespectroscopicanalysisoftheglycoprotein3. Glycoprotein release of oligosaccharides withendoglycosidases followed by purification to separateoligosaccharides enzymatic hydrolysis of purifiedoligosaccharides with specific glycosidases massspectroscopicanalysisofsmalleroligosaccharides4. Glycoprotein treat with trypsin followed by MALDIanalysesoftrypticpeptides77.Abacterialcultureundergoingbalancedgrowthrequiresafirstorder reaction. In other words, the rate of increase inbacteriaatanyparticulartimeisproportionaltothenumberormass of bacteria present at that time. If N is the number ofcellsatanygiventimetandisgrowthrateconstantthisprocesscanbeexpressedmathematicallyas(Answer1)

78. A typical animal cell (nucleated) membrane containsglycolipids and glycoproteins in the plasma membrane. Todetermine its topological distribution, lectin is used as aprobe. The following interactions may be the basis of theprobingmethod:(A)Proteinproteininteraction(B)Proteinsugarinteraction(C)Proteinlipidinteraction(D)Proteinsterolinteraction.Theappropriateansweris(Answer2)1.Only(A). 2.Only(B).3.Allof(A),(B)and(C). 4.Only(D).79.UponstudyingaconsiderablenumberofdifferentcrossesinDrosophila,Morganreachedtheconclusionthatallgenesofthisflywereclusteredintofourlinkedgroupscorrespondingtothe four pairs of chromosomes. Further studies revealed thatlinkageisnotabsoluteanditisbrokenfrequently.Itisbrokeninprophasebyaprocesscalled(Answer1)1.Recombination. 2.Jumpingofgenes.3.Integration. 4.Mutation.80.A population of proliferating cells is stained with a DNAbindingfluorescencedyesothattheamountoffluorescenceisdirectlyproportionaltothequantityofDNA.AmountofDNAineach cell ismeasuredby flowcytometry.Thenumberof cellswithagivenDNAcontent isplottedand followingstatementsweremade:(Answer1)

(1)PeakAcontainsthecellsofG1phase(2)PeakBcontainsthecellsofG2phase(3)PeakAcontainsthecellsofG2phase(4)PeakBcontainsthecellsofG1phaseWhichoftheabovementionedstatementiscorrect?1.(1)and(2). 2.(3)and(4).3.(2)and(4). 4.(1)and(3)



81.Duringmanyimportantcellprocesses,manyproteinsneedtoundergodegradationtoculminateapartoftheprocess.Forexample, during cell cycle, cycling proteins need to bedegradedtoallowthecellstoexitmitosis.Thisisachievedbyselectiveubiquitinationofcyclinfollowedbyitsdegradationbyproteasomes.Thespecificproteinfactorthatisinvolvedinthisprocess is called Anaphase Promoting Complex (APC). APC ispossiblyaproteinwhichisknownas(Answer3)1.E1enzyme. 2.E2enzyme.3.E3enzyme. 4.Protease.82. Duringmitogenic stimulation, cells proliferate at a higherrate and it is primarily determined by an enhanced rate ofprotein synthesis. Among other mechanisms, MAP kinasepathway of signal transduction is involved in this. Globalprotein synthesis may be regulated by many mechanismsinvolvingvariousstepsofproteinsynthesis,namely,initiation,elongationandtermination.Thus,manyproteinfactorsmaybeinvolved in the same. In the above process (mitogenicstimulation) the following factors are the portable targets.(Answer3)A.elF2 B.eEF1C.S6kinase D.elF4EBPThecorrectansweris1.A+B 2.C+D3.D+A 4.B+D83. DNA repair, synthesis and recombination are intimatelyconnected and inter dependent. An apparent commonalitybetween processes of DNA replication and repair in theenzymatically catalyzed synthesis of DNA polynucleotidesegments, which can be assembled with preexistingpolynucleotides, leading to repair or replication. Synthesis ofthese polynucleotide segments is catalyzed by a group ofenzymes DNAdependant DNA polymerases. In the case ofE.coli, DNA polymerase has been isolated in three distinctforms whereas five main types of polymerase have beenisolatedfrommammaliancells.Allthepolymerasessynthesizepolynucleotidesonly inthe5' 3'direction. Ifpolynucleotidechainscouldbeelongatedin3 5'direction, thehypotheticalgrowing 5' terminus, rather than the incoming nucleotide,would carry a triposphate that is unsuitable for furtherelongation. The 3' 5 exonuclease activity is not associatedwithallthepolymerasesandonlypresentin(A) All E. coli DNA polymerases but not all mammalianpolymerases.(B)PolI,PolII,PolIII,Pol,Pol.(C)PolI,PolII,Pol,Pol,Pol(D)PolI,PolII,Pol,Pol.Thecorrectstatementsare(Answer3)1.(A)and(B). 2.(A),(B)and(C).3.(A)and(C). 4.(A),(C)and(D).84. Bacteriophage genetic circuit may be represented asfollows:

The control of gene expression occurred during the phageinfectionmaybedescribedasfollows:(A)NandQproteinactasantiterminator(B)CIactsonlyasrepressor(C)CIIactasaretroregulator(D)CIandCIIbothactaspositiveandnegativeregulatorWhichoneofthestatementsarecorrect?(Answer4)1.(A),(B)and(C). 2.(B),(C)and(D).3.(A)and(D)only. 4.(A),(C)and(D).85. Rho factor is involved in termination of transcription inprokaryotes. Genetic manipulations indicate that Rhodependent termination requires the presence of a specific

recognitionsequenceonthenewlysynthesizedRNAupstreamof the termination site. The recognition sequencemust be onthe nascent RNA rather than the DNA, as demonstrated byRhos inability to terminate transcription in the presence ofpancreatic RNAse. The essential features of this terminationsite have not been fully elucidated. Construction of syntheticterminationsitesindicatesthatitconsistsof80to100ntsthatlackastablesecondarystructureandcontainmultipleregionsthatarerichinCandpoorinG.Whichofthefollowingis/aresuggestedbytheaboveobservation?(Answer1)1. Rho factor attaches to nascent RNA at its recognitionsequence and then migrates along the RNA in the 51 31direction until it encounters an RNAP paused at theterminationsite.2.RhounwindstheRNADNAduplexformingthetranscriptionbubble,therebyreleasingtheRNAtranscript.3. Rho factor attaches to theRNA at its recognition sequencewhileRNAisintheRNADNAhybridcondition.4. Theremaybe other factors andhenceRho factor doesnotneedtounwindtheRNADNAhybridtoreleasethetranscript.86.Duringdevelopmentanddifferentiation,thereisadynamicprogramme of differential expression of sets of genes. Inbacteria,phageinfectionsareamongthesimplestexamplesofdevelopmental process. Typically, only a subset of the phagegenome, offer referred to as immediate early genes, areexpressed in the host immediately after phage infection. Astime passes, early genes start to be expressed, and theimmediate early genes and bacterial genes are turned off. Inthe final stageofphage infection, theearlygenesgiveway tolategenes.Oneofthesimplestwayitisachievedisthrough(A)expressionofcascadeoffactors(B)expressionofnewRNApolymerases(C)expressionofdifferentholoenzymes(D)expressionofdifferenttranscriptionfactorsThecorrectreasonsare(Answer2)1.(A),(D) 2.(A),(C),(D)3.(A),(B),(D) 4.(A),(B),(C)87. Polyclonal antibodies are raised against bovine serumalbumin in rabbit. Subsequently IgG in the antiserum ispurifiedanddigestedwitheitherpepsinorpapain.Outof thefollowingpossibilities,whichoneiscorrect?(Answer2)1.Pepsindigestedantibodiescannotprecipitatetheantigen2.Papaindigestedantibodiescannotprecipitatetheantigen3.PepsindigestionwillproducetwoFabmolecules4. Pepsindigested antibodieswill lose all interchaindisulfidebonds88. Two protein kinases, K1 and K2 function sequentially inregulating intracellular pathway in response to extracellularsignal.Thefollowingobservationsaremade:(i) Response is observed even in the absence of extracellularsignalwhenamutationpermanentlyactivatesK1.(ii)Response isobservedeven in theabsenceof extracellularsignal when K1 contains an activating mutation and K2 withinactivatingmutation.(iii)Noresponseinthecellsisdetectedeveninthepresenceofextracellular signal when both kinases are inactivated bymutation.Whichoneofthefollowingiscorrect?(Answer4)1.K1activatesK2 2.K2activatesK13.K1inhibitsK2 4.K2inhibitsK189.Conversionofprotooncogenetooncogenemayinvolvethefollowingprocesses:AmutationincodingsequenceBgeneamplification CchromosomerearrangementDmutationinnoncodingsequenceWhichoneisappropriate?(Answer1)1.A,BandC 2.B,CandD3.A,CandD 4.All



90.Opsonisationofabacteriumisaprocessbywhichspecificantibodybindswiththesurfacemoleculeofthebacteria.Inanexperimentalcondition,macrophagewereinfectedwitheitherWT:Mycobacteriaorwithopsonised:Mycobacteriafor2hrsat370C. Subsequently, cellswerewashed and further incubatedfor24hrsat370C.Finally,bacterialloadinmacrophagesweredetermined by colony forming unit (CFU). Which of thefollowingobservationistrue?(Answer1)1.WT:Mycobacteriainhibitsitstransporttothelysosomesandsurviveinmacrophages.2. Opsonised: Mycobacteria inhibits its transport to thelysosomesandsurviveinmacrophages.3.WT:Mycobacteriaaretargetedtothelysosomesandkilledinmacrophages.4. Opsonised:Mycobateria are targeted to the lysosomes andsurviveinmacrophages.91.Whichofthefollowingcannotbeusedfordeterminationoftissue lineage of a given progenitor cell population in ananimal?(Answer4)1.Markingprogenitorcellswithvitaldye.2. Transplanting equivalent progenitor cells fromimmunologicallydistinctbutrelatedorganism.3.Markingprogenitorcellsbygenomicrecombinationcoupledwithreportergeneexpression.4.Markingprogenitorcellsbyreportergeneexpressionunderthe controlof apromoterenhancerelement specific for thegivenprogenitorpopulation.92. During vertebrate limb development, a specialisedectodermal structure, called Apical Ectodermal Ridge (AER),forms at the dorsoventral ectodermal boundary at the distaltipofthedevelopinglimbbud.ThefollowingexperimentalfactsabouttheAERisavailable:(A)FGF2,4,and8areexpressedintheAER(B)RemovaloftheAERcausescessationoflimbgrowth(C)RemovalofAERalongwithimplantationofbeadssoakedinFGF8or(D)FGF4orFGF2proteinrescuestheAERremovalphenotypeandgivesrisetonormallimbWhich of the following statements cannot bemade based ontheabovefacts?(Answer2)1.FGF2,4,and8aresecretedproteins.2.FGF2,4,and8arenecessaryandsufficientforAERfunction3.FGF2,4,and8aresufficientforAERfunction4.FGF2,4,and8havelargelyredundantfunctions93.During fertilization in amphibians, the fusion of egg andspermplasmaispreceededby(A) release of enzymatic contents from the acrosomal vesiclethroughexocytosis(B)bindingandinteractionofthespermtovitellinemembrane(C)chemoattractionofthespermtotheeggbysolublefactorssecretedbyegg(D)passageofspermthroughextracellularenvelopeWhichofthefollowingisthecorrectsequence?(Answer3)1.(A) (B) (C) (D) 2.(B) (A) (C) (D)3.(C) (A) (B) (D) 4.(C) (B) (A) (D)94. In many different contexts of cell differentiation, twodistinct cell populations emerge from a uniform cellpopulation. This process is referred to as lateral inhibition.Which one of the following must not be true about lateralinhibition?(Answer2)(A)lateralinhibitionresultsfrommorphogenaction(B)lateralinhibitionrequiresdirectcellcellcontacts(C) lateral inhibition requires reciprocal signalling betweentwoneighbouringcells(D)lateralinhibitionisprecededbystochasticchangesingeneexpressionintwoneighbouringcells1.(D) 2.(A)and(D)3.(B)and(C) 4.(A)

95.Which of the following statements is true about dorsoventralpatterningofdrosophilaembryo?(A)Thisisdictatedbythelocationofthenursecells(B)dorsalisthedefaultfate(C)Thewholeprocessisregulatedbypreventingtheentryofatranscriptionfactortothenucleusofdorsalcells(D) Homeobox containing genes play a critical role in thisprocess.(Answer4)1.(A),(B)and(C) 2.(A),(B),(C)and(D)3.(A)and(D) 4.(B)and(C)96. Which of the following is true about amphibian limbregeneration?(Answer4)(A)Itrequiresaminimumnumberoffunctionalnerves.(B)Theblastemaofanamputated limb, if transplanted in thetrunkregionbetweentwoexistinglimbsinahost,willstillgiverisetoalimb.(C)The sizeof the regenerated limb isoftengrosslydifferentfromtheoriginallimb.1.(A) 2.(B)and(C)3.(A)and(C) 4.(A),(B)and(C)97. In agamous mutant (flower within flower phenotype)whichofthefollowingstatementsisvalid?(Answer4)1.ClassAgenesareexpressedinthefirsttwowhorls,ClassBgenesareexpressedinthesecondandthirdwhorlsandClassCgenesareexpressedinthethirdandfourthwhorls.2. Class A genes are not expressed. Class B and C genes areexpressedinallthewhorls.3.ClassAgenesarenotexpressed.ClassBgenesareexpressedin the second and the third whorls and Class C genes areexpressedinallthewhorls.4.ClassAgenesareexpressedinallthewhorls.ClassBgenesareexpressedinthesecondandthethirdwhorls.98.Plantsgrowningreenhouseat25Cwhenexposedfirstto35Cfor6hoursandsubsequentlyto42Cfor12hoursadaptbetter to thehigh temperature (42C) incomparison to thosedirectlytransferredto42Cforthesameduration.Whatisthephenomenon called andwhat is itsmain physiological basis?(Answer2)1. Acquired thermotolerance because of the induction ofmutagensresultingintoimprovedstabilityofalltheproteins.2. Induced thermotolerance because of the induction of heatshockproteins.3. Induced thermotolerance because changes in RNApolymeraseIIresultinginefficientandimprovedtranscription.4. Acquired thermotolerance because of efficient posttranslationalmodificationofproteins.99.Inwhichmoleculewouldtheradiolabelappeartheearliestwhenwheatandsugarcaneleavesarefedwith14CO2?(Answer4)1.Wheatmalate,sugarcane3phosphoglycerate.2.Wheataspartate,sugarcanemalate.3.Wheat3phosphoglycerate,sugarcane3phosphoglycerate.4.Wheat3phosphyoglycerate,sugarcanemalate.100. A young dicot seedling (e.g soyabean) is subjected togravitystimulusbylayingithorizontallyonasurfacetheshootbendsupwardsandrootbendsdownward.Indicatethereason.(Answer1)1. Redistribution of auxin throughout the seedlings isresponsible for stimulatory unequal growth in shoots androots.2.Redistributionofauxininshootswhilecytokinineinrootsisresponsibleforstimulatoryunequalgrowth.3.Redistributionofauxininrootswhilecytokinineinshootsisresponsibleforstimulatoryunequalgrowth.4. Redistribution of cytokinine throughout the seedlings isresponsible for stimulatory unequal growth in shoots androots.



101.Whichofthefollowingstatementsareassociatedwiththeprocessofphotorespirationinplants?(Answer1)(A)PhotorespirationtakesplaceinonlyC3plants.(B)PhotorespirationtakesplaceinonlyC4plants.(C)PhotorespirationtakesplaceinbothC3andC4plants.(D)Glycolateisoxidizedtoglyoxylateintheperoxisome.(E)Glycolateisoxidizedtoglyoxylateinthemitochondira.1.(A)and(D) 2.(C)and(D)3.(B)and(E) 4.(C)and(E)102.Aspartatekinaseisakeyenzymeinthelysineaminoacidbiosynthesis in plants. With an objective of increasing thelysinecontent inmaizeseeds,maizeplantswere transformedwithE. coliaspartate kinasewith a strong seed specific plantpromoter. Resulting transgenic plants were found to expressthetransgene;however,thecontentoflysinedidnotincrease.Whichofthefollowingoptionbestexplainthepossiblereason?(Answer4)1.Bacterialproteinsarenotstableinplants.2.Bacterialproteinsarenotproperlyfoldedinplants.3.Properposttranslationalmodificationdidnot takeplace inplants.4.Lysinecausesfeedbackinhibitionofaspartatekinase103. Electrical stimulation of a nerve bundle maintained at3710CshowedA,B,CandDpeaks(asshowninthediagram)whenrecordedatadistanceof15cmsfromthestimulatingsiteonthesamebundle.

The same experiment was conducted at significantly lowertemperature 1510C. Which of the following statements iscorrect?(Answer3)Lowertemperaturewould1.notaffecttherecord.2.notshowA,butwouldshowB,CandDpeaks.3.notshowpeakDandmaynotshowC,butwouldshowAandB.4.showallthepeaksalongwithanewpeak104. A response was observed when a specific site in a ratbrain was stimulated by passing electrical pulses throughindwelling electrode implanted surgically. In anotherexperimentinanotherrat,acannulawassurgically implantedinstead of the electrode and stimulated the area by injectingexcitatory neurotransmitter. However, the result of the twoexperiments did not match. The possibilities of variations intheresultscouldbedueto(Answer4)1.animalvariationsonly.2.stimulationofcellbodiesornervefibersonly.3.differenceinanatomicalbrainareasonly.4.variationsinallthereasonsmentionedin1,2and3.105.(A)Inanexperiment,2mgofasubstance'A'dissolvedin4.5mlsterilesolventwasinjectedasabolusintothefemoralveinofan intact frog. It was observed that the frog's heart rateincreasedsignificantly.(B) The same solution as in (a)when applied directly on theheartofthesame frogafterexposingtheheart, theheartratedidnotchange.(C)Thesamesolutionas in (a)when injected toan intact catfemoralvein,theheartratedidnotchangesignificantly.

From the above observations, which one of the followingstatementsismostlikelytobecorrect?(Answer2)1. Substance 'A' is stimulatory on heart and the effect wasinotropic.2.substance'A'actedinthebrainandalsomighthavereleasedotherhormonestoincreasetheheartrate.3. substance 'A' could not have acted on the brain but musthave induced other substances in the blood to increase theheartrate.4.theincreasedheartratewasmerelyduetoincreasedvolumeoftheheartmuscles.106.Inahealthykidneygiventhefollowinginformation:(A)Glomerularhydrostaticpressure75mmHg(B)Glomerularcapillarycolloidosmoticpressure40mmHg(C)HydrostaticpressureintheBowman'scapsule20mnHgThenetfiltrationpressurewillbe(Answer2)1.55mmHg. 2.15mmHg.3.35mmHg. 4.135mmHg.107. Identical limb lead electrocardiograms from three adultsubjectstakenbythesamemachineunderidenticalconditionsare shown in the figure. TheXY scales should be consideredidentical for all three electrocardiograms. Which of thefollowingstatementsiscorrect?1. (a) and (c) are from normal individuals having normalhearts,while(b)showsabnormalatrialrepolarization.2. (a) is normal, (b) ventricular defect while (c) abnormalpropagationofelectricalwavesthroughatriumonly.3.(a) isnormal,(b)atrialdepolarizationisoppositetothatofinanormalheartand(c)shows localdamage throughout theventriclepossiblyduetopreviousmyocardialinfarction.4.(a)and(c)arefromnormalindividuals,havingnormalheart,whereas(b)isfromanindividualwithabnormalpropagationofelectricalwavesthroughtheventricleonly.108. Mismatch of blood in parents many result inerythroblastosisfetalisinanewborn.Matchthecorrectcause(leftcolumn)andusualtreatment(rightcolumn).(Answer3)Commonestcause UsedtreatmentA.MotherRh(+)andfatherRh() C.replacementofneonatesblood

withRh()blood.B.MotherRh()andfatherRh(+) D.replacementofneonatesblood

withRh(+)blood.1.AandC 2.AandD3.BandC 4.BandD109. In a haploid organism, the loci A/a and D/d are 8mapunitsapart.InacrossAdXaD,whatwillbetheproportionofeachofthefollowingprogenyclasses:(a)Ad(b)Recombinants.1.92%,8% 2.46%,8%3.92%,4% 4.46%,4%(Answer4)110.Duringanexperiment,aninvestigatorfoundthatthecellline used are RecA /. What could be the probable findingwhichledhimtosuchobservation?1.Lossofrecombination.2.Showingaberrantallmorphology.3.Cellsdevelopedphagocyticproperly.4.Cellswereaggregating.111.

Findthepatternofinheritanceofthetraitshowingincompletepenetrancefromthefigureshownabove.(Answer1)1.Autosomaldominant. 2.Autosomalrecessive.3.Mitochondrialinheritance. 4.Xlinkedrecessive.



112.Which of the following assumption support the HardyWeinbergEquilibrium?(Answer2)1.PresenceofNaturalSelection. 2.Randommating.3.GeneticDrift. 4.Assortativemating.113. Which of the following illustrations explain the correctpairing preceding recombination between a chromosome(ABCDEFG/ABCDEFG) and its inverted homologue(ABCDGFE/ABCDGFE). The dot in genotype represent thecentromere.(Answer3)

114. In E. coli , four Hfr strains donate the genetic markersshownintheordershown:

Strain1:FLKOZStrain2:CYAZOStrain3:PDWCYStrain4:PFLKO

AlltheHfrstrainsarederivedfromthesameF+strain.WhatistheorderofthesemarkersonthecircularchromosomeoftheoriginalE.coliF+strain?(Answer3)1.KOZPDFLWCYAK2.FLKOZPDWCYAF3.FLKOZAYCWDPF4.FLKCYAZOWDPF115. In a family, father is homozygous dominant (AA) for ageneAandhiswifeishomozygousforitsrecessiveallele(aa)showing albino phenotype. It was surprising that their childshowed the albino phenotype. Which of the followingphenomenoncanexplainthephenotype?(Answer4)1.Nondisjunction 2.UniparentalDisomy3.Geneconversion 4.Alloftheabove116. Which of the following characteristic differentiateEubacteriafromArchaebacteria?(Answer4)1.Circularnatureofchromosome.2.Absenceofnuclearmembrane.3.Presenceof70Sribosomes.4.Presenceofmureinincellwall.117. Tropical semievergreen forests are found in the regionhavingrainfall(Answer2)1.>3000mm. 2.20002500mm.3.12002500mm. 4.8001200mm.118. Presence of which of the following flora and fauna inPeninsular India is explained by the Satpura hypothesis?(Answer2)1.Chinese. 2.Malayan.3.African. 4.Mediterranean.119. Complete the following sentence using options givenbelow the sentence as a, b, c, d and e. Species are criticallyendangeredwhenitisnotendangeredbutisfacing_________riskofextinctioninthewildinthe_____________future.(Answer3)(a)high (b)veryhigh(c)extremelyhigh (d)near(e)immediate 1.a,a 2.b,c3.c,e 4.c,d

120. Based on Coefficient of Association table given below,whichofthefollowingtaxonomicphenogramofrelationshipiscorrect?(Answer4)

121.Whichoneofthefollowingtraitsetcharacterizesbestarselectedspecies?(Answer4)1. Usually a type III survivorship curve, short life span anddensitydependentmortality2. Usually a type I survivorship curve, short life span anddensitydependentmortality3.UsuallyatypeIsurvivorshipcurve,longlifespananddensityindependentmortality4. Usually a type III survivorship curve, short life span anddensityindependentmortality122.Thefollowinggraphsshowthepopulationgrowthoftwospecies P and Q, each growing either alone (a) or in thepresenceofotherspecies(b).(Answer3)

Themostimportantconclusiontobedrawnfromthegraphis1.PandQareequallycompetitive.2. In competition, the growth of both species is adverselyaffected.3.Incompetition,speciesPremainsunaffectedwhileQsuffers.4.Thereisnoevidenceofcompetitiveexclusion.123.Threeimportantbiologicalparametersgenerationtime,populationgrowth rate (r) andmetabolic ratepergrambodyweightareafunctionoftheorganismsbodysize.



Whichofthecurves(a)or(b)representsthecorrectrelationofeachoftheparameterstobodysize?(Answer3)1. Generation time (a); population growth rate (b);metabolicrate/gbw(a)2. Generation time (a); population growth rate (b);metabolicrate/gbw(b)3. Generation time (b); population growth rate (a);metabolicrate/gbw(a)4. Generation time (b); population growth rate (b),metabolicraterate/gbw(a)124. Nearly 25% of all insect species are known to beherbivores. Yet, in spite of such heavy herbivore pressure,globally green plants tend to persist, contributing to a greenearth.Whichofthefollowingaccountfortherelativesuccessofgreenplants?(Answer3)(A)Herbivoreinsectsareinefficientfeeders(B)Herbivoreinsectdensitiesarekeptlowbypredators(C)Plantssecreteherbivoredeterrentchemicals1.(B)and(C) 2.(A)only3.(B)and(C) 4.(A),(B)and(C)125.Intheprocessofnitrificationbyorganisms,therespectivebacteriaAandBinthefollowingreactionare:(Answer3)

1.Azotobacter,Nitrobacter 2.Nitrobacter,Azotobacter3.Nitrosomonas,Nitrobacter4.Nitrobacter,Nitrosomonas126.Theflightlessbirdsostrich,rheaandemuaredistributedondifferentcontinents.Whatisthemostplausibleexplanationthatisgivenbyanevolutionarybiologistforthisdiscontinuousobservation?(Answer4)1.Thebirdswereabletoflyearlier,butlosttheirflightabilitylater2. Prehistoric humans transported these birds to differentcontinents3.Thebirds,althoughflightless,mayhaveuseddriftinglogstocrosstheoceanandreachothercontinents4. All the continents used to be one single supercontinentearlierandtheflightlessbirdswereisolatedafterthebreakupofthelandmass.127. Inspite of its twofold cost, sexual reproduction is themost dominant mode of reproduction among the livingorganisms.Which of the following reasonsmight account forthis?(A) Sexual reproduction generates genetic heterogeneitythroughrecombination(B)Sexualreproductionhelpsinpurgingdeleteriousmutations(C)Sexualreproductionevolvedtostayevolutionarilyaheadoffastevolvinginternalparasites.(Answer4)1.(A)only 2.(A)and(B)3.(C)only 4.(A),(B)and(C)128. Amoth species occurs as two distinctmorphsbased onwingcolourpaleanddark.Intheforesttherearetreeswithdark coloured trunks as well as those with light colouredtrunksandthemothscanrestoneithertree.Birdscapturetheresting moths and eat. In a field experiment, the proportion(%) of dark and pale morphs captured from dark and lighttrunktreeswasrecorded.(Answer4)

Themostplausibleconclusiontobedrawnfromtheresultsis

1.Naturalselectionfavoursdarkmorphsinforestswheretreeswithdarktrunksaredominant.2.Birdscandetectdarkmorphsbetterthanlightmorphs.3.Palemorphsprefertorestonlightcolouredtrunks4.Birdsdetectthemothsbycuesotherthantheirwingcolour.129. It is found that people with the genetic disease calledsickle cell anaemia are resistant to malaria. Which of thefollowing best describes the underlyingmechanism? (Answer2)1.Frequencydependentselection2.Superiorityofheterozygotes3.Transientpolymorphism4.Balancedpolymorphism130. In a bird species A, the male alone builds the nest,incubatestheeggsandfeedsthenestling.InbirdspeciesB,itisthe female that does all that. In bird species C, both sexescontribute equally to the above activities. In speciesA andB,the uninvolved partnermay fly away andmate again.Whichsex among A, B and C is most likely to develop colourfulplumageduringbreedingseason?(Answer4)1.MaleinspeciesAandB,bothsexesinspeciesC.2.FemaleinspeciesA,maleinspeciesBandC.3.FemaleinspeciesAandB,neitherinspeciesC.4.FemaleinspeciesA,maleinspeciesB,neitherinspeciesC.131.Ifagivengeneinarandomlymatingpopulationhasthreealleles a, b and c in the ratio of 0.5, 0.2 and 0.3 respectively,what is theexpected frequencyofgenotypesabandbc in thepopulationatequilibrium?(Answer3)1.0.1and0.06 2.0.2and0.153.0.2and0.12 4.0.04and0.09132.Thefollowingtablelistssomeoftheenzymesoffungiandbacteria having wide variety of industrial applications,including alcoholic beverages, food, detergents andpharmaceuticals,alongwiththeirmicrobialoriginalEnzyme MicroorganismA.Amylase E.AzotobactervinelandiiB.Asparginase F.SerratiamarcescensC.Lipase G.AspergillusaureusD.Pectinase H.AspergillusoryzaeThecorrectcombinationsare(Answer1)1.AandH 2.BandG3.CandE 4.DandF133.Animalcellculturesarefrequentlyusedforproductionoftherapeutic proteins. NIH3T3 (a fibroblast cell line) and CHO(Chinesehamsterovariancellline)aresomeofthepopularcelllines used. Choose the best combination of cell line (fortransfection) and starting material for purification of humangrowthhormone,asecretaryprotein(Answer4)1.NIH3T3cellpellet 2.CHOandcellpellet3.NIH3T3andculturemedium4.CHOandculturemedium134.Anovelvaccine(againstmalaria liverstagespecific)hasbeen developed by an investigator. This happened to be theparasites unique cell surface protein molecule (Mp). This Mpwheninjectedinmiceelicitshumoralantibodyresponse(IgGinnature)andcanefficientlyneutralizesporozoitesbyinhibitingtheir binding to the liver cells. Upon a preclinical trial thisvaccine failed universally despite a high titre IgG response.Which one out of the choices below is the correct answer ofthisfailure?(Answer2)1.ThelivercellsurfacereceptortowhichMpbindsischanged.2.ThemalariaparasiteissuccessfulinchangingtheepitopeinMptowhichIgGbinds3.IgGmoleculeschangeitsbindingsitesforMp.4.TheaffinityofIgtoMpepitopesisreduced.



135. Following are some statements about Agrobacterium plantinteractions(A) Agrobacterium transfers a part of its chromosome intoplantcell.(B)Agrobacterium transfers apart ofoneof itsplasmidDNAintoplantcell.(C)AllthevirulencegenesofAgrobacteriumareinducible.(D) All the virulence genes of Agrobacterium are functionalonlyinsidethebacterialcells.(E)SomeofthevirulencegenesofAgrobacteriumareinducible.(F) Some of the virulence genes of Agrobacterium arefunctionalbothinbacterialandplantcells.Which of the following combination of statements is true?(Answer2)1.(A),(C)and(D) 2.(B),(D)and(E)3.(C),(D)and(E) 4.(B),(E)and(F)136. To generate a knockin or a knockout mouse, it isessentialtouseantibioticselectiontoselectthestemcellsthathave putatively integrated the construct accurately. Eachconstructwillhave5homologyarmand3homologyarmtohelp in homologus recombination. A positive antibioticselection cassette and a negative antibiotic selection cassettearealsoused.Negativeselectioncassettehelpsindeselectionof nonhomologous recombinant stem cells. Choose onestatement indicatedbelowthatreflects thecorrectpositionofthenegativeselectioncassette.(Answer4)1. Negative selection cassette is supplied in a separateconstruct.2. Negative selection cassette is placed between the twohomologyarms.3. Negative selection cassette is placed 3 to the 3homologyarm.4.NegativeselectioncassetteisinsertedbetweenthepromoterExonIofpositiveselectioncassette.137.Followingarethedifferentwaysofobtaininghumanstemcells(A)Cells frommorula aredispersedand cultured to give risepluripotentstemcells.(B) Isolated inner cell mass of a blastocyst are cultured andtheybecomepluripotentstemcells.(C) The primordial germ cells from a fetus are collected andcultured,whichgrowintopluripotentstemcells.(D) Stem cells are collected from adult tissues and grown inspecificmannertodevelopintopluripotentstemcells.Whichoftheabovecelltype(s)isextensivelyusedforsomaticcellgenetherapyinhuman?(Answer4)1.(A)and(B) 2.(B)only3.(B)and(D) 4.(D)only138. RFLP and RAPD markers are commonly used in plantbreeding and diversity analysis. Which of the followingcombination of statement about these molecular makers arecorrect?(Answer3)A.RFLPiscodominant B.RAPDiscodominantC.Boththemarkersareubiquitous D.OnlyRFLPcandetectheterozygoteE.OnlyRAPDcandetectheterozygoteF.RAPDcannotdetectallelicvariation1.A,C,D,E 2.B,C,E,F3.A,C,D,F 4C,D,E,F139.WhichoneofthefollowingindicatestepsinvolvedinDNAfootprinting to monitor interaction of DNA with a protein?(Answer2)1. DNA + protein treat with restriction enzymes runagarosegel stainwithethidiumbromideandviewunderUVlight.2. Label one of theDNA strandswith a radiolabel treat oneportionoflabelledDNAwiththeproteinofinterestfollowedby

reactionwithDNAse treat secondportion ofDNAwith onlyDNAse.RunboththetreatedDNAsamplesonasequencinggel.3.Analyzefragmentsobtainedfromtheexperimentsdescribedin(2)byMALDImassspectrometry.4.DNA+protein treatwithDNAse followedby restrictionenzymes. Run the sample on SDS page and visualize bycommassiebluestaining.140. A student is asked to isolate and purify varioussubcellular organelles from a liver cell sample. Followingsucrose density gradient centrifugation technique, and takingutmostcaremitochondrialfractionisfoundtobecontaminatedwithnuclearfraction.Whichtechniqueoutofthechoicesgivenbelow should be appropriate to obtain a cleanmitochondrialfraction?(Answer1)1.Gelfiltration 2.Percolldensitygradient3.Immunomagneticseparation4.Pulsefieldelectrophoresis141.During receptormediatedendocytosis, ligand firstbindswith cell surface receptor, then traffic through Rab5 positiveearlyendosomalcompartment.Finally, itmovestotheLamp1positive lysosomes via Rab7 positive late compartment. InordertounderstandthetraffickingofligandAinepitheliacells,cellswereallowedtointernalizeligandAforvariousperiodoftimes at 37C. Finally, cells were stained with antiligandantibody and probed with secondary antibody labelled withAlexaRed fluorescence dye. Same cells were also costainedwithantiRab5,antiRab7orantiLamp1antibodyandprobedwithappropriatesecondaryantibodylabelledwithAlexagreenfluorescence dyes. Cells were viewed in confocal microscopeandobservationsare(I)5mininternalizeligand(Red)incellsarecolocalizewithantiRab5antibodybutnotwithantiLamp1antibody and (II) 90 min internalized ligand are colocalizedwith antiLamp1 antibody but not with antiRab5 antibody.The following conclusions couldbearrivedat from theaboveobservations.(Answer3)(A)LigandAtravelstoearlyendosomalcompartmentby5min.(B)LigandAtravelstoearlyendosomalcompartmentbyabout90min.(C)LigandAtravelstolysozomebyabout5min.(D)LigandAtravelstoearlyendosomebyabout90min.Identifythecorrectinferences.1.(A)and(B) 2.(B)and(D)3.(C)and(D) 4.(D)and(C)142.Thevaluesofmolarabsorptioncoefficient()ofTrpandTyrat240nmand280nmarethefollowing:

A 10mg sample of a protein is hydrolyzed to its constituentamino acids and diluted to 100 ml. The absorption of thissolutionina1cmpathlength,is0.717at240nmand0.239at280nm. The estimated content of Trp and Tyr in mol / gproteinrespectivelyare(Answer4)1.586and28.1. 2.58.6and281.3.586and281. 4.58.6and28.1.143.ThestructureofaproteinisknownfromXraydiffractionstudies which gave 30% helix, 50% sheet and 20%randomcoil.Circulardichroism(CD)measurementsgave50%helix,40%sheetand10%randomcoil.Whatcouldnotbeapossibleexplanationfortheseobservations.(Answer1)1.Proteinstructure in thecrystal isdifferent fromthat in thesolution.2.CDanalysisforstructuralcomponentsisnotappropriateforthisprotein.3. Contributions from other chromophores also contribute totheCDspectrumoftheprotein4.Proteincontainshighcontentofdisulphidebonds.



144.IncolumnIaregivenequationsandincolumnIIwhattheequationsrepresent.ColumnIandIIareincorrectlymatched.

The correct match in sequence of the four equations fromcolumnIIis(Answer1)1.b,d,aandc 2.d,b,candd3.b,d,canda 4.d,b,aandc145. It is claimed that themean () arsenic concentration inthegroundwaterofvillage is20g/Lwith=3.Inarandomsample of 16 measurements what values of arsenicconcentration should lead to rejection of the claimwith 95%confidence?(Answer4)1.valueslowerthen18.53andvalueshigherthat21.472.valueshigherthan21.473.valueslowerthan18.534.valueslowerthan18.07andvalueshigherthan21.93The Solutions has been provided by IFAS, Jodhpur.UtmostcarehasbeentakenwhilesolvingpaperbutIFAS Jodhpur does not take responsibility for anymistakesinanswerkeyprovided.Wehavepreparedsolution using best of our information but you areadvised tocheckanswerswith reputed sources.Thepurposeofprovidingsolution istohelpthestudentswhoarepreparing for thisexam.Wewish themallthebestforexam.Acknowledgement: Paper has been taken fromofficialwebsitefromhttp://csirhrdg.res.in.Wethankthem. It isbeingused fornonprofitpurposeand isavailablefreeofcostonourwebsitetoallstudents.Foranyqueryorsuggestionregardingsolutions.Feelfreetomailat:[email protected]

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