Date post: | 13-Jan-2017 |
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Culture characteristic of bacteria
Prepared bySamira Fattah
Assis. Lec.College of health sciences-HMU
Lab 6
• Bacteria grow on solid media as colonies.• A colony is defined as a visible mass of
microorganisms all originating from a single mother cell.
Colony Morphology• These characters used to accurately describe the morphology
of a bacterial colony:-Size-Shape-Color (also known as pigmentation)-Texture-Height (elevation)-Edge (margin)
• Key features of bacterial colonies are important criteria for their identification.
COLONY CHARACTER ON AGAR PLATE
• Colony shape on agar plates
Rhizoid
Circular Pinpoint
• Margin of bacterial colony
Undulate filamentous
• Elevation of bacterial colony
Raised marginUmbonate
• Size of the bacterial colony
• Small• Medium• large
• Texture of bacterial colony
• Dry• Moist• Viscid (stick to loop)• Mucoid (mucus-like)
• Colour of the colonies (pigmentation) Some bacteria produce pigment when they grow in the
medium.
• Opacity of the bacterial colony
• Opaque (not clear)
• Translucent (clear)
• Iridescent(shine)
COLONY CHARACTER ON AGAR SLANT
• Growth on agar slants
beadedFiliform
GROWTH IN NUTRIENT BROTH
• Growth in nutrient broth
LAB EXERCISE
-Student will identify the growth patterns on the nutrient agar plates and slants, and in the nutrient broth tubes.