CURRICULUM VITAE 1. Name: Dr. Chandan Mandal
2. Designation: Administrator of I3T Advance Diagnostic Center and Principal Scientist
3. Job responsibility: Smooth running of advance diagnostic center which includes Hematology, Clinical
Biochemistry, Serology, Histopathology, Cytogentics, Flow cytometry, Molecular genetics (Real time
PCR and Nextgen sequencing) in NABL standard work flow and Drug development, Translational
Glycoimmunology, Proteomics and Glycomics mainly in cancer and infectious disease as Principle
Scientist
4. Place of work: International Institute of Innovation and Technology, Kolkata
5. Record of Achievement
SL
NO
Subject Institute
1 COVID-19: Operational Planning Guidelines
and COVID-19 Partners Platform to support
country preparedness and response
World Health Organization (WHO) and Health
Emergencies Programme
2 Health Cluster Coordination (Global Health
Cluster)
World Health Organization (WHO) and Health
Cluster
3 Decontamination and sterilization of medical
devices
World Health Organization (WHO) and Health
Emergencies Programme
4 Standard precautions: Environmental cleaning
& disinfection
World Health Organization (WHO) and Health
Emergencies Programme
5 Standard precautions: Waste management World Health Organization (WHO) and Health
Emergencies Programme
6 Introduction to the Principles and Practice of
Clinical Research
National Institutes of Health (NIH) and
Department of Health and human service-USA
6. Academics:
Research Institution Year
Scientist C Tata Medical Center, Kolkata June 2016-Dec2019
Special Appointed Researcher (Post Doctorate)
Immunoglycobiology Division, IFReC, Osaka University, Japan
2014-Aug to 2016 June
Post Doctorate Indian Institute of Chemical biology, CSIR, India 2009-2014 (Aug)
Ph. D.* (Life Science.) Jadavpur University, West Bengal, and Indian Institute of Chemical biology, CSIR, India 2009
M. Sc. (Organic. Chem.) CSJMU, Kanpur, India 2002
Member of National Society: Member of Association of Carbohydrate Chemist Technologists (India)
Life Member: Kolkata Gynecological Oncology Trials and Translational Research, Kolkata
List of Publications
1. Chowdhury S, Bhattacharya K, Mandal Chandan, Mondal S, Sarkar S, Chandra S, Banerjee S, Mandal C.A
Glycomic Approach Towards Identification of Signature Molecules in CD34+ Haematopoietic Stem Cells
from Umbilical Cord Blood. Adv ExpMed Biol. 2018; 1112:309-318. doi: 10.1007/978-981-13-3065-0_21.
https://www.ncbi.nlm.nih.gov/pubmed/30637706
2. Satyavarapu EM, Das R, Mandal Chandan, Mukhopadhyay A, Mandal C. Autophagy-independent induction
of LC3B through oxidative stress reveals its non-canonical role in anoikis of ovarian cancer cells. Cell Death
Dis. 2018 Sep 17;9(10):934. doi: 10.1038/s41419-018-0989-8.
https://www.ncbi.nlm.nih.gov/pubmed/30224639
3. Sarkar Bhattacharya S*, Mandal Chandan*, Albiez RS, Samanta SK, Mandal C. Mahanine drives pancreatic
adenocarcinoma cells into endoplasmic reticular stress-mediated apoptosis through modulating sialylation
process and Ca2+-signaling. SciRep. 2018 Mar 2;8(1):3911. doi: 10.1038/s41598-018-22143-w. *Equal
contribution https://www.ncbi.nlm.nih.gov/pubmed/29500369
4. Chandan Mandal, Sayantani Sarkar, Reinhart SA, Mandal C. GD3 synthaseoverexpression inhibits survival
and angiogenesis of pancreatic cancer cells through S-phase arrest and disruption of β1 integrin-mediated
anchorage. Int J Bio Cell Bio. 2014, 53:162-73, ISSN: 1357-2725
http://www.ncbi.nlm.nih.gov/pubmed/24842107 (Citedby 10)
5. Chandan Mandal, Chhabinath Mandal, Sarmila Chandra, Roland Schauer and ChitraMandal. Regulation of
O-acetylation of sialic acids by sialate-O-acetyltransferase and sialate-O-acetylesterase activities in
childhood acute lymphoblastic leukemia. Glycobiology. 2012, 22, 70-83 (Cited by 7)
http://www.ncbi.nlm.nih.gov/pubmed/21803834 (Cited by 10)
6. Chandan Mandal, Cristina Tringali, Susmita Mondal, Leugi Anastasia, Sarmila Chandra,Venerando Burno
and Chitra Mandal. Down-regulation of membrane-bound Neu3 is negatively correlated with disease
progression and associated with apoptosis suppression of lymphoblasts in childhood acute lymphoblastic
leukemia. International J Cancer. 2010, 126:337-349 (Cited by 40)
http://www.ncbi.nlm.nih.gov/pubmed/19588508
7. Chandan Mandal, G. Vinayaga Srinivasan, Suchandra Chowdhury, Sarmila Chandra,Chhabinath Mandal,
Roland Schauer, and Chitra Mandal. High Level of Sialate-O-acetyltransferase Activity in Lymphoblasts of
Childhood Acute Lymphoblastic Leukaemia (ALL): Enzyme Characterization and Correlation with Disease
Status. Glycoconjugate Journal. 2009, 26, 57-73 (Cited by 20)
http://www.ncbi.nlm.nih.gov/pubmed/18677580
8. Chandan Mandal, Avijit Dutta, Asish Mallick, Sarmila Chandra, Laxminarain Misra,Rajender Sangwan, and
Chitra Mandal. Withaferin A induces apoptosis by activating p38 mitogen-activated protein kinase signaling
cascade in leukemic cells of lymphoid and myeloid origin in a transcription-dependent manner through
mitochondrial death cascade. Apoptosis. 2008, 13, 1450-1464 (Cited by 133)
http://www.ncbi.nlm.nih.gov/pubmed/18987975
9. Susmita Mondal, Chandan Mandal, Chandra S, Mandal C. Upregulated alfa2,6 sialyltransferases in
association with reduced Neu3 promotes cell survival in pediatric leukemia. Exp. Cell Res. 2020
(communicated)
10. Sayantani Sarkar, Chandan Mandal, Sangwan R, Mandal C. Coupling G2/M arrest to the Wnt/β-catenin
pathway restrains pancreatic adenocarcinoma. Endo.Rel.Cancer 2014, 21(1):113-25
http://www.ncbi.nlm.nih.gov/pubmed/24402132
11. Chowdhury S, Chandan Mandal et. al. Mobilization of lymphoblasts from bone marrow to peripheral blood
in childhood acute lymphoblastic leukaemia: Role of 9-O-acetylated sialoglycoproteins. Leukemia Research.
2012 Feb;36(2):146-55 http://www.ncbi.nlm.nih.gov/pubmed/22078066
12. Khatua B, Ghoshal A, Bhattacharya K, Chandan Mandal, Saha B, Crocker PR, Mandal C. Sialic acids acquired
by Pseudomonas aeruginosa are involved in reduced complement deposition and siglec mediated host-cell
recognition. FEBS Lett. 2010, 584, 555-61. (Cited by 36) http://www.ncbi.nlm.nih.gov/pubmed/19945458
13. Susmita Mondal, Chandan Mandal, Sangwan R, Chandra S, Mandal C. Withanolide D induces apoptosis in
leukemia by targeting the activation of neutral sphingomyelinase-ceramide cascade mediated by synergistic
activation of c-Jun N-terminal kinase and p38 mitogen-activated protein kinase. Mol Cancer. 2010, 9, 239.
(Cited by 45) http://www.ncbi.nlm.nih.gov/pubmed/20836852
14. Kankana Mukherjee, Anil Kumar Chava, Chandan Mandal, Sailendra Nath Dey, Bernhard Kniep, Sarmila
Chandra, Chitra Mandal. O-acetylation of GD3 prevents its apoptotic effect and promotes survival of
lymphoblasts in childhood acute lymphoblastic leukaemia. J. Cell biochem, 2008, 105, 724-34 (Cited by 19)
http://www.ncbi.nlm.nih.gov/pubmed/18655184
15. Chowdhury S, Bandyopadhyay S, Chandan Mandal, Chandra S, Mandal C. Flow-cytometric monitoring of
disease-associated expression of 9-O-acetylated sialoglycoproteins in combination with known CD antigens,
as an index for MRD in children with acute lymphoblastic leukaemia: a two-year longitudinal follow-up study
BMC Cancer, 2008, 8, 40 (Cited by 12) http://www.ncbi.nlm.nih.gov/pubmed/18241334
16. Mukherjee, K Chowdhury S, Mondal, S, Chandan Mandal, Chandra S, Bhadra RK and Mandal C 9-O-
acetylated GD3 triggers programmed cell death in mature erythrocytes. Biochemical and Biophysical
Research Communications, 2007, 362, 651-657 (Cited by 13)
http://www.ncbi.nlm.nih.gov/pubmed/17761143
Chapter in Book Series 1. Chandan Mandal and Chitra Mandal. Identification and analysis of O-acetylatedsialoglycoproteins. “Methods
in Molecular Biology” Chapter 6, Human Press, Springer publishing group, USA, 2013 981:57-93
http://www.ncbi.nlm.nih.gov/pubmed/23381854
2. Kankana Mukherjee, Suchandra Chowdhury, Susmita Mondal, Chandan Mandal, Sarmila Chandra, and Chitra
Mandal 9-O-Acetyl GD3 in lymphoid and erythroid cells, MICC-3 book, Publisher Springer, NY, USA, Adv Exp
Med Biol 2011 705, 317-334. http://www.ncbi.nlm.nih.gov/pubmed/21618115
Poster Presentation International Conference
1. Poster presentation titled “High Level of Sialate-O-acetyltransferase Activity in Lymphoblasts of Childhood
Acute Lymphoblastic Leukaemia (ALL): Enzyme
Characterization and Correlation with Disease Status” in International symposium on cell surface
macromolecule, held on January, 2005 at Puri, India
2. 1st prize in poster presentation titled “Evaluation of membrane bound Neu3 in childhood acute
lymphoblastic leukemia” in International symposium named Glycan 2006, in Indian institute of science,
Bangalore, India
3. Poster presentation titled, Withaferin A induces apoptosis by activating p38 mitogen-activated protein
kinase signaling cascade in leukemic cells of lymphoid and myeloid origin in a transcription-dependent
manner through mitochondrial death cascade” in International symposium on cell surface macromolecule,
held on January, 2008 at Centre for Cellular and Molecular Biology, Hyderabad, India
4. International symposium named, International symposium on Chemical Biology, held on March, 2007 at
Indian Institute Chemical Biology, Kolkata, India
5. Oral presentation on “Down regulation of membrane-bound Neu3 constitutes a new potential marker for
childhood acute lymphoblastic leukemia and induces apoptosis suppression of neoplastic cells” in the
conference of Society for Biological chemist held at Digha 2009
6. Poster Presentation on “Regulation of O-acetylation of sialic acids by sialate-O-acetyltransferase and
sialate-O-acetylesterase activities in acute lymphoblastic leukemia” in International symposium named
“Sialoglyco2010” held at Potsdam, Germany dated Aug. 2010.
7. Poster presentation on “Elevated level of GD3-induced program cell death in pancreatic cancer” in
International symposium “SIGNALING NETWORK AND CANCER” held at IICB, Kolkata, dated Feb. 2011.
8. 1st Annual meeting of Kolkata Gynecological Oncology Trials and TranslationalResearch, Kolkata,
Biswabangla Convention center
Techniques known
Analytical tools: Thin layer Chromatography (TLC), High performance TLC (HPTLC), GelFiltration, Affinity
Purification, Ion exchange chromatography, High performance liquid chromatography (HPLC), Radio HPLC,
Fluorimetric HPLC, MALDI-TOF, Nano-HPLC-ESI-QTOF,
Imaging: Fluorescence activated cell shorting (FACS) and analysis. Fluorescence Microscopy, Confocal
microscopy. Ratiometric confocal microscopy, Transmission Electron Microscopy (TEM), Scanning Electron
Microscopy (SEM) Histopathology.
Molecular genetics: RNA isolation, Real Time PCR, Gene micro-array, Recombinant DNA Technology, CRISPR-
Cas9 system, GECKO library, Haploid genetic screening, Split protein technology etc.
Biobank: Primary culture and Cell line development, Organoid development. Primary tissue storage for whole
genome, transcriptome and proteomics analysis
Animal work: Animal handling (mice, Rat, Rabbit) (feeding, injection, blood collection,spleen cell
isolation, peritoneal macrophage isolation etc for mice.), pharmacokinetics, tumor formation in NUDE mice.
Protein & Lipid characterization: Protein purification, Electrophoresis (1D &2D), Proteinsequencing,
Enzyme characterization, Radio Immuno assay, Western blotting, Immuno-precipitation, Lipid purification and
analysis by TLC.
Exposure to various instruments
(a) HPLC (b) MALDI-TOF (c) LC-MS (d) FACS (e) TEM (f) Confocal Microscopy.
Development of new ELISA method for sialate-O-acetyltransferase activity Mandal et.al. Glycobiology. 2012, 22, 70-83
Doctorate details
Title of Ph.D. Thesis : Enzymatic modulation of sialic acid in childhood acute lymphoblastic leukemia
Research Supervisor: Chitra Mandal, PhD.
FTWAS, F.N.A.,F.A.Sc, F.N.A.Sc, Sir J.C. Bose National Fellow Head, Cancer and Cell Biology Division Outstanding Scientist, Sct-H CSIR-Indian Institute of Chemical Biology, Kolkata Email : [email protected], [email protected] Tel :91-33-2473-3491 Mob : +919831036964
Brief outline of Doctoral research Research Work : [I] Regulation of O-acetylation of sialic acids by sialate-O-acetyltransferase and sialate-O-acetylesterase
activities in childhood acute lymphoblastic leukemia.
[ii] High Level of Sialate-O-acetyltransferase Activity in Lymphoblasts of Childhood Acute Lymphoblastic
Leukaemia (ALL).
[iii] Down-regulation of membrane-bound Neu3 is negatively correlated with disease progression and
associated with apoptosis suppression of lymphoblasts in childhood acute lymphoblastic leukemia.
[iv] Down-regulation of membrane-bound Neu3 is negatively correlated with high Sialate-O-
acetyltransferase Activity in Lymphoblasts of Childhood Acute Lymphoblastic Leukaemia.
[v] Withaferin A induces apoptosis by activating p38 mitogen-activated protein kinase signaling cascade in
leukemic cells of lymphoid and myeloid origin in a transcription-dependent manner through
mitochondrial death cascade.
Brief outline of 1st Post-doctoral research work:
[i] GD3 induce program cell death in pancreatic cancer.
[ii] It induces S-phase cell cycle arrest in pancreatic cancer.
[iii] It disrupts integrin-beta 1 signaling pathway.
[iv] GD3 inhibit the tumor angiogenesis
Brief outline of 2nd Post-doctoral research work:
[i] VAPs modulate the membrane contact sites organisation.
Visit abroad:
Visiting Research Associate
Charite Universitatmedizin,
Berlin, Germany
Aug 2012 to Nov 2012
Identification sialoglycoproteins by Mass Spectrometry and Cellular proteome analysis by MALDI-TOF and, Nano-HPLC-ESI-QTOF in cancer
Visiting Research Fellow
DKFZ, Heidelberg, Germany
2010 Glyco-immunology, Tumor angiogenesis
(Sialo-enzymes)
Special Appointed Immunoglycobiology 2014-Aug to Regulation Lipid and Calcium
Researcher Division, IFReC, 2016 June transfer through membrane
Osaka University, contact sites
Japan
Reference:
Dr. Chitra Mandal, F.N.A.,F.A.Sc,F.N.A.Sc.,
Director.Distinguished Biotechnology research Professor
Cancer Biology & Inflammatory Disorder CSIR-Indian
Institute of Chemical Biology, Kolkata Email:
[email protected], [email protected] Tel :91-33-
2473-3491
Dr. Sib Sankar Roy
Principle scientist, CSIR-IICB, Kolkata
Cell Biology & Physiology
Email: [email protected]
Phone: 9433414568
Family details
Father’s name: Basudev Mandal
Mother’s name: Sibani Mandal
Date of birth: 15th September, 1978
Sex: Male
Marital status: Married
Nationality : Indian
Email:[email protected];
Phone: 9433128906
Permanent address: At-Putputia, Tamluk, Purbamedinipur, West Bengal India, Pin-721651