Cystatin C, Lipoprotein(a) and Ceruloplasmin in the Thermo Scientific Konelab Test Menu

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Specific Protein tests on Konelab Systems

Albumin

Antistreptolysin O

-1-Antitrypsin

Apo A1 & B

Complement C3

Complement C4

C-reactive protein

High Sensitive CRP

HbA1c

Haptoglobin

IgA, G & M

Microalbuminuria

Myoglobin

Orosomucoid

Prealbumin

Rheumatoid factors

Transferrin

• Cystatin C• Lipoprotein(a)• Ceruloplasmin

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Renal testing

• Creatinine, Enzymatic

• Creatinine, Jaffé

• Urea/BUN

• Total protein in urine

• Microalbuminuria

• Cystatin C

Source: http://training.seer.cancer.gov/module_anatomy/unit11_2_uri_components.html

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Cystatin C - a Novel Serum Marker for GFR

• Kidney monitoring• GFR, Glomerular Filtration Rate• Traditionally with Creatinine measurement• NEW MARKER – CYSTATIN C

• Why Cystatin C together or instead of Creatinine?• Specificity

• Creatinine is affected by age/sex/muscle mass/inflammatory process

• Cystatin C test is not dependent of any listed issues• Sensitivity

• Creatinine measurement is able to detect kidney dysfunction when app. 50% of kidney mass is ‘non-working’

• Cystatin C test has the sensitivity to detect already mild renal dysfunction

• Other highlights• Elevated serum Cystatin C is a strong predictor of the risk of

death and cardiovascular events in elderly persons

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Cystatin C on Konelab systems

• Easy and reliable assay• Ready for use liquid reagents

• Method: latex enhanced immunoturbidimetry• On board stability for reagents 30 days• Kit size 2 x 3 ml, up to 190 tests• 981911 Cystatin C

• One level stock calibrator• Ready for use liquid• Automatic dilutions for calibration curve• Kit size 2 x 1 ml• 981912 Cystatin C Calibrator

• Controls• Normal and elevated levels• 981913 Cystatin C Control, 3 x 1 ml• 981914 Cystatin C Control High, 3 x 1 ml

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Lipid tests

• Total Cholesterol

• HDL & LDL-Cholesterol

• Triglycerides

• Apolipoprotein A1 & B

• Lipoprotein(a)

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• Sterols: Cholesterol, steroid hormones etc.

• Fatty acids

• Glycerol esters, e.g. triglycerides

• Sphingosines and Terpenes• e.g. Vitamin A, E and K

Classification of Lipids

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• Synthesized in liver• 10% coming from dietary intake

• Transported in complexes known as lipoproteins• Clinical interest in HDL and LDL Cholesterol

Triglyceride+

Esterified Cholesterol

Phosp

holip

id

Free Cholesterol

Apolipoprotein

Cholesterol

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Triglyceride+

Esterified Cholesterol

Phosp

holip

id

Free Cholesterol

Apolipoprotein

Lipoproteins

• Apolipoprotein A-I/II => HDL: “good cholesterol”

• Apolipoprotein B => LDL: “bad cholesterol”

• Lipoprotein(a) = LDL- molecule + Apolipoprotein (a)

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Lipoprotein(a)

• A powerful predictor for development of atherosclerotic vascular disease

• Lipoprotein(a) is a dimer consisting of LDL molecule bound to Apo(a) via a disulphide bridge

• Lp(a) is a risk factor for coronary heart disease (CHD) that is independent of all other lipid parameters

• Lp(a) is a special tool for doctors• The CHD risk predictor is high, especially

when Lp(a) and LDL are elevated simultaneously => lipid lowering therapy recommended

Lipoprotein(a) – a ’killer molecule’

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Lipoprotein(a) on Konelab Systems

• Easy and reliable assays• Ready for use liquid reagents

• Method: direct immunoturbidimetry• Kit size 1 x 1 ml, up to 180 tests• 981915 Lipoprotein(a)

• One level stock calibrator• Ready for use liquid• Automatic dilutions for calibration curve• 981916 Lipoprotein(a) Calibrator, 2 x 1 ml

• Controls• Normal and elevated levels• 981917 Lipoprotein(a) Control, 3 x 1 ml• 981918 Lipoprotein(a) Control High, 3 x 1 ml

k

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Ceruloplasmin

• Ceruloplasmin measurement aids to diagnose copper metabolism disorders

• Ceruloplasmin is the major copper–containing protein in plasma

• When is the Ceruloplasmin level abnormal?• Decreased levels

• disorders of copper metabolism like Menke’s disease and Wilson’s disease

• Elevated levels• During pregnancy• With lymphoma• In acute and chronic infections (acute phase protein)• Rheumatoid arthritis

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• Ready for use liquid reagents• Method: direct immunoturbidimetry• On board stability for reagents 30 days• Kit size 2 x 3 ml, up to 190 tests• 981919 Ceruloplasmin

• SpeciCal for calibration• Ready for use liquid• Automatic dilutions for calibration curve• 980997 SpeciCal, 5 x 1 ml

• Controls• 981250 SpeciTrol, 5 x 1 ml• 981799 SpeciTrol High, 5 x 1 ml

Ceruloplasmin on Konelab Systems

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Albumin ACPTot & Dir Bil ALPBicarbonate ALT/GPTCalcium AST/GOTCholesterol AmylaseHDL&LDL Chol CHECreatinine DibucaineGlucose CKIron CK-MBMagnesium Gamma-GTPhosphorus -HBDHTotal Protein LDHTriglycerides LipaseU/CSF ProteinUrea ELECTROLYTESUric Acid Na, K, Cl

Ca, pH

Konelab Menu – over 80 carefully validated methods

SU

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Konelab Menu – over 80 carefully validated methods

Albumin

Antistreptolysin O

-1-Antitrypsin

Apo A1 & B

Complement C3

Complement C4

C-reactive protein

High Sensitive CRP

HbA1c

Haptoglobin

IgA, G & M

Microalbuminuria

Myoglobin

Orosomucoid

Prealbumin

Rheumatoid factors

Transferrin

sTfR

Cystatin C

Lipoprotein(a)

Ceruloplasmin

Amphetamine

Barbiturates

Benzodiazepines

Cannabinoids

Cocaine Metabolite

Opiates

Cotinine

Ethyl Alcohol

Methadone

Propoxyphene

CarbamazepinePhenobarbitalPhenytoinTheophyllineValproic AcidLithium

SP

EC

IFIC

PR

OT

EIN

S

Do

A (

DA

T)

TD

M

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Konelab System Reagents

• Proven, continously expanding test menu

• Delivered in optimal package sizes to serve different sized laboratories

• Ready for use liquid reagents for ease of use