Tissue Regeneration Activator

D+Cell 350 TRA is the dermal care product for the professional use only with PDRN which has treated for wound and ulcer as a medicine. As well, D+Cell 350 TRA is optimized for various skin care and treatment processes, and furthermore the efficacy and safety were approved through clinical studies by dermatological experts.

: Mesotherapy, Trandermal use, Scar, Recovering dermis & Scalp

: Fraxel Laser, IPL, MTS, Iontophoresis, Electroporation

: Local Whitening

: After peeling, Homecare after treatment

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What is ?

Outstanding characteristics of

D+CELL 350 TRA is the skin revitalization treatment that other products and treatments up to now cannot do without. That is because D+CELL 350 TRA with cell generation and cell growth-promotion ingredients offers the perfect way to activate excel-lently fast and comparable skin tissue regeneration. Moreover, as these ingredients were already approved and have been used for the medicals, D+CELL 350 TRA can be safe, and also specifi-cally can be suited for use in more sensitive skin areas.

1. Regernate dermal tissues

- Increase the number of fibroblast in dermis

- Promote to secrete the substances as like fibroblast, of which skin composed.

- Generate new capillaries in skin and improve the microvascular circulation

- Provide scarless wound healing without hypertrophy by feedback system.

- Decrease swelling by anti-inflammatory and anti-edemic effects

2. Maintain the tissue regeneration activation and conditions for longer than any other does

3. Prevent cells from skin aging and recover damaged cells by promoting DNA biosynthesis

Control : Dulbecco’s modified Eagle’s medium(DMEM) with 1% fetal calf serum

PDRN : Dulbecco’s modified Eagle’s medium(DMEM) with 1% fetal calf serum + 10ug/ml PDRN

Ref. S. Thellung et al, Polydeoxyribonuleotides enhancethe proliferation of human skin fibroblasts, Life science Vol 64. No 18 1661-1674, 1999

Fibroblast proliferation Dose dependent Fibroblast proliferation

Cell growth activation by promoting DNA synthesisPDRN, as tissue regeneration activator (TRA), activates the proliferation of fibroblasts in demis, and this effect is dose-dependent in a certain dose range.

A2 Pur ine receptor agonist

Salvage pathway stimulation

Mechanism of action (Tissue regeneration activator)

Stimulation on various growth factorsPDRN, as a tissue regeneration activator (TRA), interacting with A2 purinergic receptor stimulates the secretion of various growth factors as below.

VEGF

Vascular endothelial growth factor (VEGF) is known a kind of growth factor as an important signal-ing protein to stimulate a new blood vessel formation and growth. VEGF is a part of restoration system for oxygen supply when blood circulation is inadequate.

bFGF

During wound healing, various enzymes activate basic fibroblast growth factor (bFGF), thus promoting the growth of fibroblasts, mediating the formation of dermal tissues and stimulating capillary generation.

IGF-1

Insulin-like growth factor (IGF-1) through AKT pathway plays an important role in promoting cell growth and proliferation of muscles, cartilage, nerve and skin, and can delay program cell death.

Angiopoietin

Angiopoietin is a protein growth factor to promote the formation of blood vessels from pre-existing blood vessels unlike VEGF.

IL-8

IL-8(Interleukin-8) which is a chemokine signals other immune cells to come in to the site of inflam-mation, and mediates these immune cells can reduce the inflammatory response.

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- Tissue regeneration solution

- Esthetic regeneration solution

D+CELL 350 TRA I vitalizes the regeneration and growth of tissues in dermis. And these actions induce to fill up the depressed tissues of the dermis and allow to re-fomulate the skin tissues into healthy conditions.

A variety of factors such as aging, UV and exposure to many chemical materials occur the reduction of fibroblasts and their activities. This reduction results in the decrease of components for skin and accordingly low skin elasticity. Different from skin cell vitalization by temporally stimulation or moisturizing, D+CELL 350 TRA II gives the proliferation of fibroblast to restore and boost skin elasticity and vitality. This treatment of TRA II can prolong the revitalizating effects due to the proliferation of fibroblast. That is the reason why D+CELL 350 TRA II is the ideal skin boosting treatment which nurtures the skin in a way that many other creams and moisturizers simply cannot do.

D+CELL 350 TRA I activates the secretion and proliferation of fibroblast. The increase of fibroblast produces various components of dermis such as collagen, elastin, reticular fiber and GAGs (Glycosaminoglycans), and these components enlarge dermis as a result. Furthermore, its advantageous effects can be maximized when it is treated with proper processes using in cosmetic and plastic operation.

Also, D+CELL 350 TRA I improves skin elasticity in scalp, and specilly promotes the formation of a blood vessel as well as the growth of hair bulb.

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TRA II can be used in conjunction with other aesthetic treatments such as CO2 fraxel laser, NdYag laser, IPL, MTS, FNS, chemo peel, etc. This skin conditioning approaches can provide more adaptable treatment for those special occasions to look the best.

Typically, D+CELL 350 TRA W contains arbutin, which was identified for whit-ening effect from KFDA, and placental extract to inhibit the activity of tyrosi-nase as a core element for the bio-synthesis of melanin pigments.

UV affects to the skin basal layer to make the keratin layer further thicker and causes more secretion of melanin pigment to make skin tone much darker. Also, as UV lessens the vital-ization of skin, it enhances more skin photo-aging. However, as the proliferation of fibro-blast caused by D+CELL 350 TRA W induces the regeneration and more vital activities of skin cells and tissues, it can help dead skin cells drop out and keratin layer become thinner, then to recover and improve skin tone.

D+CELL 350 TRA Cream is skin regeneration cream which includes PDRN, tissue regenera-tion activator. PDRN is reported to assist the production of the components of the extra-cellular matrix. This considerable property of PDRN makes it the best choice obviously for skin areas that take on a dehydrated and less vital appearance with over exposure to the UV as well as physical and chemical responses.

W - Regeneration & Whitening solution

Cream – Maintenance cream

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① P. Sini et al., Effect of polydeoxyribonucleotides(PDRN) on human fibroblasts in primary culture: Cell Biochemistry and FunctionVol.17 1999, 107 - 114

② Thellung S et al., Polydeoxyribonucleotide(PDRN) enhances the proliferation of human skin fibroblasts: Involvement of A2Purinergic receptor subtypes: Life Sciences Vol.64 No.18, 1999, 1661 - 1674

③ S. Guizzardi et al., Polydeoxyribonucleotide(PDRN) promotes human osteoblast proliferation: A new proposal for bone tissuerepair: Life Sciences Vol.73 2003, 1973 - 1983

④ A. Bitto et al., Polydeoxyribonucleotide(PDRN) improves angiogenesis and wound healing in experimental thermal injury: CriticalCare Vol.36, No.5 2008, 1594 - 1602

⑤ M. Galeano et al., Polydeoxyribonucleotide(PDRN) stimulates angiogenesis and wound healing in the genetically diabeticmouse: Wound Repair and Regeneration Vol.16 2008, 208 - 217

⑥ Rubegni P et al., Clinical evaluation of the trophic effect of polydeoxyribonucleotide(PDRN) in patients undergoing skin explants:Current Medical Research and Opinion Vol.17, No.2 2001, 128 - 131

⑦ G. D. Aloe et al., Skin graft donor site and use of polydeoxyribonucleotide as a treatment for skin regeneration: A randomized,controlled, double-blind, clinical trial: WOUNDS Vol.16, No.8 2004, 1 - 6

⑧ G. Magi et al., Clinical assessment of the effect of polydeoxyribonucleotide in trophic lesions of the lower limbs in chronicvasculopathic patients: Minerva Angiologica Vol.16, No.2 1991, 437 - 440

⑨ M. C. Masnada et al., Sull’impiego di un estratto di placenta per via intradermica in lesion acneiche cicartriziali: La MedicinaEstetica Vol.11, No.2 1987, 69 - 71

⑩ M. Vigano., Utilizzo di un polodesossiribonucleotide(PDRN) nelle ustioni di grado intermedio: Giornale Italiano di RicercheCliniche e Terapeutiche Vol.11, No.5 1990, 90 - 93

⑪ L. Valdatta et al., Evaluation of the efficacy of polydeoxyribonucleotides in the healing process of autologous skin graft donorsites: Current Medical Research and Opinion Vol.20, No.3 2004, 403 - 408

⑫ 김요한 등, 전층피부 결손에서 PDRN의 창상치유 효과, 대한성형외과학회지, 제37권, 제3호,. 220 - 226, 2010

STERILE 2.2ml × 5 Vials

30g

4F Won-il Bldg., 1429-8 Seocho-Dong, Seocho-Gu, Seoul 137-070, KoreaTel: 82-2-451-7640 Fax: 82-2-451-7647www.pharma-research.co.kr

References

I Tissue regeneration solution

II Esthetic regeneration solution

W Regeneration & Whitening solution

Cream Maintenance cream