PROTEOMICS

Rapid Measurement of Lung Cancer Biomarkers

Ambermarie Dawdy

August 01, 2016

Jin H. Park, Biochem J., 2012,448(Pt 3): 417-423

Why Should we Study Lung Cancer?

Rebecca L. Siegel, CA CANCER J CLIN 2016,66, 11-18

Estimated New Cases

Prostate 21% Lung & Bronchus 14%Colon & Rectum 8%Urinary Bladder 7%Melanoma 6%Lymphoma 5%Kidney & Renal 5%Oral & Pharynx 4%Leukemia 4%Liver & Bile Duct 3%

Lung & Bronchus 27%Prostate 8%Colon & Rectum 8%Pancreas 7%Liver & Bile Duct 3%Leukemia 4%Esophagus 4%Urinary Bladder 4%Lymphoma 4%Brain & Nervous 3%

Breast Lung & Bronchus Colon & Rectum Uterine corpus Thyroid Lymphoma Melanoma Leukemia Pancreas Kidney & Renal

Lung & BronchusBreast Colon & Rectum Pancreas Ovary Uterine corpus Leukemia Liver & Bile Duct Lymphoma Brain & Nervous

Estimated Deaths

29%13%

8%7%6%4%3%3%3%3%

26%14%

8%7%5%4%4%3%3%2%

*17,369 Cases of estimated new cases in Florida and 11,960 estimated deaths from lung cancer

What are Biomarkers?• Measurable substances in

organisms whose presence is indicative of a biological occurrence such as disease/infection

• Tissue microarray is used to remove sections of tumor tissue and clinical biopsies

• Reduces the time it takes to process tissue microarrays from 3 hours a sample to 12 minutes a sample

Mascaux C, Clin Cancer Res, 2011,17, 7801

Methods

Cellularity Biomarker: Lamin-A/C

Library A549 H1648 H1650 H1975 H322 HCC2279 HCC827

Lamin Protein Structure

SGAQASSTPLSPTR

Quigley A, Science ,2013,339:1604-1607

Cellularity Biomarker: Epidermal Growth Factor Receptor

Library A549 H1648 H1650 H1975 H322 HCC2279 HCC827

.LTQLGTFEDHFLSLQR

EGFR STRUCTUREErlotinib Structure

SUMMARY

● Lung Cancer has Poor Patient Outcomes

● Improved assessment of biomarkers can help the patient

● Goal: Develop and implement new methods for biomarker detection

Acknowledgments

Funding: Frank E. Duckwall Foundation, Saunders Foundation, Moffitt Cancer Center

Koomen LabJohn Koomen, PhDMelissa MartinezOmar Chavez-Chiang, MD

Proteomics CoreBin Fang, PhDLancia Darville, PhDVictoria IzumiKiah Bowers

SPARKDoug Cress, PhDEvelyn Rivera-Miranda

Moffitt Cancer Center Moffitt Foundation