Department of Genetics, Microbiology Department of Genetics, Microbiology and Toxicology (GMT)and Toxicology (GMT)
Radiation BiologyRadiation Biology
Prof. Andrzej WojcikProf. Andrzej Wojcik
Prof. Mats Harms-RingdahlProf. Mats Harms-Ringdahl
ToxicologyToxicology
Prof. Thomas HelledayProf. Thomas Helleday
Prof. Ulf Rannug Prof. Ulf Rannug
Prof. Dag JenssenProf. Dag Jenssen
Centre for Radiation Centre for Radiation
Protection ResearchProtection Research
(CRPR)(CRPR)
Molecular Mechanisms Molecular Mechanisms
in Cancer in Cancer
/ Dag Jenssen, GMT
Radiation Biology (AW & MHR)
2 Profs2 Profs
11 Postdoc Postdoc
55 PhD students PhD students
22 Master students Master students
MMechanismechanisms of cellular and systemics of cellular and systemic
effects of exposure to ionising radiationeffects of exposure to ionising radiation
EU grantsEU grantsSwedish Radiation Safety AuthoritySwedish Radiation Safety Authority
Radiation Biology (AW & MHR)
Mechanisms Mechanisms of individual sensitivity toof individual sensitivity toionising radiation: ionising radiation:
• Differences in DNA-damage response pathways between Differences in DNA-damage response pathways between cohorts of patients with normal or severe healthy tissue cohorts of patients with normal or severe healthy tissue reactions to tumour therapy.reactions to tumour therapy.•Screening for polymorphisms in genes for DNA-repair, cell Screening for polymorphisms in genes for DNA-repair, cell cycle regulation and apotosiscycle regulation and apotosis
• Search for diagnostic tools to identify the persons at risk.Search for diagnostic tools to identify the persons at risk.
Patient with severe side-effect
following radiotherapy
Radiation Biology (AW & MHR)
What What areare the risk the riskss of radiation at low doses of radiation at low doses??
CaCann we improve biological dosimetry? we improve biological dosimetry?
New technologies will determine doses with high precision?New biomarkers for DNA-damage response pathways
Epidemiological data
(above background)
(excesscancers)
Is the dose response curve Is the dose response curve
non-linear?non-linear?
Identification of new responseIdentification of new response
pathways for very low doses.pathways for very low doses.
??
Professor Thomas Helleday’s group
SU groupSU group
1 Prof1 Prof
10 Postdocs & senior scientists10 Postdocs & senior scientists
8 PhD students8 PhD students
1 Master students1 Master students
DNA repair in cancer therapyDNA repair in cancer therapyMain grants from:Main grants from:
Swedish Scientific Research Council (VR)Swedish Scientific Research Council (VR)
Swedish Pain Relief Foundation (Smärtafonden)Swedish Pain Relief Foundation (Smärtafonden)
Swedish Cancer Foundation (Cancerfonden)Swedish Cancer Foundation (Cancerfonden)
Swedish Children’s Cancer Foundation (Barncancerfonden)Swedish Children’s Cancer Foundation (Barncancerfonden)
Oxford groupOxford group
6 Postdocs6 Postdocs
7 DPhil students7 DPhil students
Niklas Schultz, Helge Gad, Fredrik Johansson, Anne Lagerkvist,Niklas Schultz, Helge Gad, Fredrik Johansson, Anne Lagerkvist,
Linda Savolainen, Ingegerd Elvers, Cecilia Ström, Petra Groth, Linda Savolainen, Ingegerd Elvers, Cecilia Ström, Petra Groth,
Firas Al-Ubadi, Ivaylo Stoimenov, Kristian Grimberg, Guoli Liu, Firas Al-Ubadi, Ivaylo Stoimenov, Kristian Grimberg, Guoli Liu,
Muntasir MamunMuntasir Mamun
Research areasResearch areas• Stalling of Replication fork and Replication RestartStalling of Replication fork and Replication Restart• Homologous recombinationHomologous recombination• DNA replication terminationDNA replication termination• DNA repair in cancer (cells + clinical material)DNA repair in cancer (cells + clinical material)
SU - DNA Repair team
SU - Biochemistry/proteomicsAnn-Sofie Jemth, Olga LosevaAnn-Sofie Jemth, Olga Loseva
Research areasResearch areas• Poly (ADP ribose) polymerasesPoly (ADP ribose) polymerases• Proteomics after DNA damageProteomics after DNA damage• Purification of protein targets for cancer treatmentPurification of protein targets for cancer treatment
SU – Cancer BiologyTatjana Djureinovic, Evgenia GubanovaTatjana Djureinovic, Evgenia Gubanova
Research areasResearch areas• DNA damage signalling in cancerDNA damage signalling in cancer• Evaluation of targets for cancer treatmentEvaluation of targets for cancer treatment
SciLifeLab - High-Throughput Screening
Bo LundgrenBo Lundgren
Research areasResearch areas
• RNAi library screens in cellsRNAi library screens in cells
• Identify targets for cancer treatmentsIdentify targets for cancer treatments
• Map functional genetic networksMap functional genetic networks
SU – Medical Chemistry
Martin Scobie, Tobias KoolmeisterMartin Scobie, Tobias Koolmeister
Research areasResearch areas
• Lead optimisation - Parallell organic chemistry synthesisLead optimisation - Parallell organic chemistry synthesis
Professor Ulf Rannugs group
1 Prof1 Prof
1 Postdoc1 Postdoc
3 PhD students3 PhD students
1 Master student1 Master student
Natural non-persistent bioactive Natural non-persistent bioactive aryl hydrocarbon receptor ligands aryl hydrocarbon receptor ligands in humansin humans
The The Swedish research Council FORMASSwedish research Council FORMAS
Professor Ulf Rannug, cont.
NH
HN
O
CYP1
H2N CHC
CH2
OH
O
HNTrp N
H
HN
OOH
NH
HN
OOH
HO
AhRE
CHEMICAL INHIBITORS
AhRAhRNFNFĸĸBB
Formation and metabolism of
the suggested physiological
Ah receptor ligand 6-formyl-
indolo[3,2-b]carbazole (FICZ)
HPLC, LC/MS/MS
Recombinant human enzymes
Human cells in vitro
Human blood, plasma and urine samples
Professor Ulf Rannug, cont.
TRYPTOPHAN PROLIFERATION/APOPTOSIS
DEVELOPMENT
DIFFERENTIATION
HOMEOSTASIS
IMMUNE RESPONS
INFLAMMATION
NEUROENDOCRINE
FUNCTIONS
STRESS RESPONSES RESPONSES
Genomic and
non-genomic
regulationARYL HYDROCARBONRECEPTOR (AhR)
SECONDMESSENGERS
OTHER RECEPTORS (LXRR)
FICZActiveligand
oxFICZInactiveligand
CYP1 AOX
TRYPTAMINE
I-3-A
The new AhR paradigm
Professor Dag Jenssen’s group
2 Profs2 Profs
2 Postdocs2 Postdocs
2 PhD students2 PhD students
3 Master students3 Master students
Molecular/cellular mechanisms for induced Molecular/cellular mechanisms for induced mutationsmutations EU grantsEU grantsSwedish Cancer SocietySwedish Cancer SocietySwedish Radiation Protection AuthoritySwedish Radiation Protection Authority
/ Dag Jenssen, GMT
/ Dag Jenssen, GMT
Professor Dag Jenssen, cont.
Mechanisms involved in the repair of genomic Mechanisms involved in the repair of genomic DNA intra-strand cross-links (Cf, 2010-2013)DNA intra-strand cross-links (Cf, 2010-2013)
The basis for cancer risk assessment of lifestyle factorsThe basis for cancer risk assessment of lifestyle factors
Cell culture modelsCell culture modelsDNA repairDNA repair
DNA replicationDNA replication
Mutation assayMutation assay
DNA sequencingDNA sequencing
Professor Dag Jenssen, cont.
Molecular/cellular protection mechanisms against Molecular/cellular protection mechanisms against carcinogenic constituents in food (EU, 2010-2014)carcinogenic constituents in food (EU, 2010-2014)
The basis for developing Functional FoodsThe basis for developing Functional Foods
/ Dag Jenssen, GMT