DEPARTMENT OF PHYSICLOGYANNUAL REPORT

July 1, 1966 - June 30, 1967

ACADEMIC STAFFE. B. Brown, Jr., Ph. D., Professor and ChairmanAlan M. Thompson, Ph. D., ProfessorDonald Johnson, Ph. D., Associate ProfessorG. N. Loofbourrow, Ph. D. , Associate ProfessorL. P. Sullivan, Ph. D., Associate Professor

Alan Goodridge, Ph. D., Assistant ProfessorKenneth L. Goetz, Ph. D., Assistant ProfessorJames Rhodes, M. D., Assistant ProfessorJohn W. Trank, Ph. D., Assistant ProfessorA. Stark Wolkoff, M. D., Assistant Professor.

POSTDOCTORAL FELLOWS

Woo Gyeum Kim, M. D., (International Atomic Energy Agency Fellow)Seoul National Univ., Korea, 9-1-64 to 8-30-66, returned to Korea.

Norberto Gonzalez, M. D., National Res. Council Fellow, Argentina,and Cardiovascular Res. Training Grant Fellow, 10-1-65 to 9-30-67.

Helen Starke, M. D., CV Res. Training Grant Fellow, 7-1-66 to 6-30--67.James Scherbenske, Ph. D. ,CV Res. Train. Grant Fellow, 9-1-66 to 6-30-67.

Tomi-O Hishida, 9-1-65 to 8-30-66, returned to Nagoya Univ., Nagoya, JapanTakuma Hojo, M. D., 9-1-65 to 8-8-67, return to Tokyo Medical College,

Department of Surgery, Tokyo, Japan.

GRADUA TE STUDENTS

Ray CookLarry WellingPaul HensleighConrad Johanson

Barbara McDougalMartin Kramen

Dennis WaringGary BondSam SteeleRichard MorrisonCleve HowardEdward MartinFrank ScammanJohn Sun

Carol Hendley

Degree SoughtM.S.Ph. D.Ph.D.Ph. D.Ph.D.Ph.D.Ph. D.Ph.D.Ph. D.Ph. D.M.A.Ph. D.Ph. D.Ph.DPh.D.

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KAW VALLEY HEART STUDENTS: (The Science Talent Search Program,summer 1966)

Conice LaddMariella CellettiDave Heuber

MEDICAL STUDENTS - Summer Research Fellows

Ron OelschlagerLarry Rothman.Ted GnauWarren JohnsonRobert VarnumIra Cox

Robert StephensonBo b BoydKent StocktonRick BrabbeePaula Elliott

TEACHING:

The Physiology Course for Medical Students was again taught during thewinter and spring quarters, with the last two weeks of the year beingdevoted exclusively to the combined course in Endocrinology.

Graduate Courses taught during the past year were:#320 - Fluid, Electrolyte, and Acid-Base Metabolism - Dr. Brown, spring '67.#325 - Biology of Sex - Dr. Johnson, fall 1966.#327 - Physiological Instrumentation - Dr. Trank, Winter 1966.

Graduate Seminars #351 offered during the year were:Fall: Dr. Thompson, "Cerebral Blood Flow and Metabolism. if

Wint er: Drs. Brown and Thompson gave a joint seminar on "History of Physio!. ifSpring: Dr. Trank gave a seminar on "Bioinstrumentation. :1

Nurses Course in "Basic Human Biology" was again taught in the summer of 1966by the combined departments of Biochemistry, Anatomy, and Physiology.Dr. John W. Trank and graduate students Kramen, Cook and McDougal,all from the Department of Physiology, and Medical Students Dan Weaver,James Fitzsimmons, Mark Kadyk and Ronald Thomas participated in thisjoint effort.

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Many staff members of other departments at KUMC gave PhysiologyDepartment Seminars.

VISITORS AND OUTSIDE SPEAKERS: The following visiting professorspresented lectures during the year:

Sept. 20, 1966

Sept. 22, 1966

Oct. 24, 1966

Jan. 5, 1967

Jan. 30, 1967

Feb. 13, 1967

AprillO, 1967

Dr. David Jensen - Scripps Institutional Oceanography,Univ. of Calif., San Diego. I The Aneural Heart of theHagfish. .,

R. O. H. Irvine, Sr. Lecturer in Medicine, Univ. of Otago,Medical School, Dunedin, New Zealand, "Muscle Cell pH inMetabolic Acidosis. .1

Dr. Eugene D. Robin, Pulmonary Div., Dept. of Medicine,.Univ. of Pittsburgh, Pittsburgh, Penn,sylvania. "A Trilogy:

"Active" Transport of CO2 - Humoral Control of PulmonaryCirculation - pH in Cold (and hot) Blood. iI

Dr. Richard L. Clancy, Cardiology Branch, NIH"Myocardial Fiber Shortening Velocity and OxygenConsumption. "

Dr. James Brierly, Neurophsychiatric Research Unit,Medical Research Council Lq,boratories, Carshalton, England."The Physiology and neuropathology of decreased cerebralblood flow in Rhesus monkey and in man. .,

Dr. Ken Armitage, Department of Zoology, University ofKansas, Lawrence, Kansas. "Effect of photo period onmolting and reproduction in the crayfish. "

Dr. K. Brown-Grant, Locke Research Fellow of the

Royal Society, Department of Human Anatomy, Oxford,England. fA New Action of Progesterone. I'

Several professors from the Lawrence Campus presentedlectures in our department during the year.

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Th~ department of physiology was strengthened considerably during

the past academic year by the addition of two new faculty members. Dr.

Alan Goodridge joined the department after two years as a postdoctoral

fellow in the laboratories of Dr. Eric Ball at Harvard. By virtue of order-

ing equipment and supplies ahead of his arrival, he got his research program

underway in record time. He has obtained research grants from the

American Cancer Society and the National Institutes of Health and has

completed work for two publications since his arrival. Dr. Goodridge gave

the lectures on metabolism in the physiology course for first-year medical

students. Dr. James Rhodes joined the department with a joint appointment

in Medicine after postdoctoral work at the University of Chicago. Dr.

Rhodes' interest is G. 1. Physiology and Clinical Gastroenterology. He joins

Dr. Arthur Klotz in the Gastroenterology Section of the Department of

Medicine and has his research laboratory in connection with Dr. Klotz's

laboratories on the first floor of Wahl Hall East. Dr. Rhodes gave the

lectures on G. 1. Physiology in the course for first-year medical students.

Both Drs. Goodridge and Rhodes directed one of the sections of the labora-

tory work of the physiology course for first-year medical students.

For the first time this year laboratory teaching space for physiology

and pharmacology was combined. This was accomplished by dividing the

large teaching laboratory in the Pharmacology department into smaller

rooms,;ind using two of these for student teaching along with two of the

larger rooms in the Physiology department which are assigned for student

teaching. If this had not been accomplished in time for the course to begin

in January. this department would have been unable to accotT~modate the

larger class in the laboratories this year. The absolute maximum capacity

of our laboratories prior to combining with pharmacology was 64 students.

Since the class is divided into two sections, this had meant a maximum class

size of 128 students. During the past academic year we had 140 students

registered for credit in the freshman physiology course; 124 medical students

and 16 graduate students. By combining space with the pharmacology depart-

ment we now have a capacity in the laboratory of 72 students with the room

comfortably filled and a possible capacity of 76 with the rooms crowded.This means a maximum class size of 144 to 152 students. With the slow

but steady growth of graduate prograffis in the basic ffiedical sciences, it

is fairly clear that this size class will be reached very soon. even if no

further increase in the nUffiber of entering first-year medical students

occurs. Furtherffiore, the number of seats in WHE auditorium is 156,

and with the number of faculty meffibers and graduate students not registered

for credit who attend lectures, the lecture hall was filled to capacity during

the past academic year. All of these considerations point to the fact that one

of the pressing needs of the immediate future for this medical center is that

of a building to house the student teaching functions of the first two years.

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ACTIVITIES OF STAFF:

Dr. E. B. Brown, Jr., Professor and Chairman

Editor: Proc. Soc. Exp. BioI. Med., ending September 1966.Modern Medicine, ending December 1966.

Member of American Physiological Society Finance CommitteeKaw Valley Heart Association, Chairman, Research Committee

Research and Education Committee, VA Ho spital,K. C., Mo.

Outside lectures presented:

Kaw Valley Heart Summer Students on ilGenesis of a Research Program"

Postgraduate Nursing Program on Facing Death, talked on "The

Physiological Process of Dying. ,I

Pediatric Cardiology Group in December 1966.

A series of five lectures given on acid-base physiology for the

Department of Anesthesiology on Tuesdays and Fridays during the winter qtr.

Participated in Medicine Grand Rounds on Basic Acid-Base Disturbances

in Respiratory Failure, December 1966.

Emporia State Teachers College, Emporia, Kansas, December 1966.

Ten lectures at California Medical College, Los Angeles, Feb. 6-17, 1967.

Seminar, Dept. of Physiology UCLA, February 1967.

Postgraduate Medicine's Eastern Circuit Decerr.iber 6-9, 1966, and

Western Circuit January 10 -13, 1967.

Postgraduate Symposium on Inhalation Therapy May 20, 1967, "Review

of the Physiology of the respiratory system. ;J

Meetings Attended:

American Physiological Society meetings and APS Finance Committee

meeting August 15-Sept. 3, 1966.

International Congress of Nephrology, Washington, D. C., Sept. 25 -3O, 1966.

Am. Physiological Society meetings in Chicago, Illinois,April 16-21, 1967.

Site Visit for Nat. Heart Institute, NIH, to Dept. of Physiology, Universityof Wisconsin, Sept. 21, 1966.

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Dr. Alan M. . Thompson, ProfessorReview Editor for papers submitted to Science Magazine.Member of KUMC Animal Care Committee

Attended the Cerebrovascular workshop in Miami in January'1967.

Lectured in Los Angeles for one week to students in the CaliforniaMedical College on transport.

FASEB meetings in Chicago in Spring 1967.

Participated in Graduate Student Procurement Trips to William JewellCollege at Liberty, Missouri, and Central Missouri State College atLiberty, Missouri, and Central Missouri State College at Warrensburg,Missouri.

Was host to a group from Kansas State Teacher's College (Caduceus Society)Emporia, Kansas, on December 8, 1966, here on the KUMC Campus.

Dr. Donald C. Johnson

Attended meetings of Comparative Endocrinologist (American Soc. Zool.)Madison Wisconsin, November 11-13, 1966.

Federation meetings in Chicago April 17-21, 1967.

Participated in joint program in Reproductive Physiology withvarious departments at Kansas State University and the LawrenceCampus which started back in the summer of 1965.

Dr. G. N. Loofbourrow

Was again active in the Neurology Section of the Department of Medicine,Division of Neurosurgery, and the Basic Science Departments, and gave aseminar on the Limbic System, May 19, 1966.

Consultant to VA Hospital, K. C. Missouri

Also continues to read manuscripts for Dr. E. Gellhorn, ProfessorEmeritus of Neurophysiology, University of Minnesota.

Graduate Student Procurement Trips to Atchison, Kansas. St. Benedicts Collegeand Parkville, Missouri, Central Missouri State College.

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Talk on Physiological Basis of the Electroencephalograffis atPediatrics Grand Rounds, October 25, 1966.

Participated in Clinical Correlation Conf. (Physiology-Neurosurgery)3/4/67.

Demonstration of Nerve Action Potentials to two groups under aegisof the Regional Council on Higher Education 2 -12 -66.

Served on three Ph. D. Committees for Graduate Students in the

Department of Anatomy.

Dr. L. P. Sullivan

Reviewed papers for J. Pharmacology.

Attended 3rd International Congress of Nephrology, Washington, D. C.,Sept. 1966.

Participated in elective correlation conference for first-year students.

Member of KUMC Research Committee.

Is in the second year of his Career Development Award, July 1, 1966.

Attended APS meetings in Spring 1967.

Principal advisor Dr. J. Scherbenske, NIH Postdoctoral Fellow.

Became member of American Nephrology Society.

Gave paper on Renal ammonium countercurrent system at meeting ofKUMC Sigffia Xi Club.

Lr. Alan G. GoodridgeParticipated in Graduate Student Procurement trips to Tabor College,Hillsboro, Kansas, Sterling College at Sterling, Kansas, and McPher sonCollege at McPherson, Kansas.

Was seminar speaker at University of Michigan, Department of Zoology,in October 1966. "The effect of prolactin on lipogenesis in the pigeon. .1

Consultation with Dr. B. E. Frye. .

Seminar speaker - Physiology Department, KUMC, "The effect of prolactin

on lipogenesis in the pigeon," Oct. 1966, and again in March 1967 on"The effect of insulin and glucagon on the in vitro metabolism of adiposetissue from chick embryos and growing chicks. iJ

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Participated in a conference onlEcology of Fat" at the University ofGeorgia, Athens, Georgia, April 1967.

Attended the FASEB meetings in Chicago, Illinois, in April 1967.

Dr. Kenneth L. Goetz:

Attended American Heart Association annual meeting in New Yorkin October, 1966.

Attended the F ASEB meetings in Chicago, April, 1967.

Gave a series of three lectures to Pediatric Cardiology group in

Spring, 1967.

Gave a talk on Ventricular Contractility to adult cardiology group inSpring 1967.

Completed requirements for M. D. degree, June 1967.

Served as consultant to Midwest Research Institute.

Dr. James Rhodes

Attended (AGA) American Gastroenterology Association meeting inColorado Spring, May 1967.

Attended FASEB rr.eetings April!, 1967, in Chicago.

Participated in several medical, surgical and pediatric conferences

on clinical problems in GI Physiology.

Participated in Saturday rr.orning Elective Courses in GI to correlate

the basic science and clinical aspects.

Lectured to physicians at St. Luke's Hospital in K. C. Mo. on the

physiological and clinical aspects of total colectorr.y and ileostomyin Noverr.ber 1966.

Lectured in Internal Medicine Postgraduate Course on the Physiology ofChronic Active Liver Disease in November 1966.

Participated in Wednesday Physiology Laboratory for freshman students.

Lectured on Protein digestion and absorption at Hines VA Hospital in

ChicagoinJuly1967.

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Dr. John W. Trank, Assistant Professor

Attendeu a meeting in Chicago of the Task Force on Vibration of floorsystems in steel-framed buildings of the engineering subcommittee ofthe Committees Structural Steel and Steel Plate Producers.

Visited Dr. Oppenheimer at Temple University on June 26,27,28, 1966,to serve as a consultant on the design of an experimental procedureinvolving recording electrical activity from small sensory nerves.

Attended a meeting of the Task Force on Vibration of Floor Systemsin New York, August 24, 1966.

On 9/13/66 attended a meeting in Aspen, Colorado, on CardiovascularControl Systems.

November 20, 1966, presented a paper at the Technology UtilizationConference in Houston sponsored by NASA.

Graduate Student Procurement Trips to Ottawa University, Ottawa, Kansas,and Emporia State College of Emporia, Emporia, Kansas, and BakerUniversity at Baldwin, Kansas.

Consultant to Midwest Research Institute, Kansas City, Missouri.

Dr. A. Stark Wolkoff

Conoucted Post Graduate Seminar in Obstetrics and Gynecology anopresented two papers:

"The effects of anesthesia on the unborn lamb. "

:'Negative aspects of the intra-uterine contraceptive oevice. "

Presented a paper to the Tulsa Obstetrics and Gynecology Society meetingNovember 23, 1966, titled "Alterations in the Intra-uterine fetal blood gases.'

Presented a paper on i'l\/ledical information needed by personnel and guidancecounselors." to the Am. Assoc. of Personnel and Guidance Counselors,Dallas, Texas, March 19-23, 1967.

Attended the Society of Gynecological Investigation Convention in NewOrleans, March 1-4, 1967.

Presented to Am. College of Obstetrics and Gynecology Ppril 17 -20,Breakfast Conference on the 'Intra-uterine fetal environment. II

Correlated Seminar dPlacental function. iI

:, The physiology of the placental' presented in the colloquy.

1967,

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Was visiting professor at TripIer General Hospital in Honolulu, Hawaii,on Cctober 25, 1966. Presented a lecture titled "Post maturity andplacental function. "

Presented "'Physiological concepts of the placenta" at the Kapiolani

Hospital in Honolulu, Hawaii.

. Was visiting Professor at the University of the Philippines for one monthfrom May to June, 1967.

Presented lecture to tre State Family Planning meeting in Salina, Kansas,

dealing with the "New concepts in contraception. I

Took part in the Western Circuit program for the Post Graduate Departmentat the University of Kansas Medical Center.

RESEARCH

Research grants which were active during the year were the following:

\ Intracellular co mpensatory changes in response to chronic respiratory acidosisand alkalosis. Air Force Contract #AF 41(609)-3005, USAF School of Aerospace

Medicine, Brooks Air Force Base, Texas. Principal Investigator: E. B. Brown,Jr.Jan. 1, 1966 to Dec. 31, 1966. $18,662. Jan. 1, 1967 to Dec. 31, 1967, $17,068.

Potassium metabolism and acid-base balance. USPHS #5 ROI AM 05649.

Principal Investigator: E. B. Brown, Jr., June 1, 1966 to May 31, 1966. Extendedwithout additional funds to November 3(" 1966. Supplement for equipmentpurchase $13,500.

Tissue CO2 chemical forms and exchange rate. USPHS #GM 10429. PrincipalInvestigator: Alan 1:vI. Thompson. Sept. 1, 1964-Aug. 31, 1965. Extended toAugust 31, 1966 without additional funds. $14,400.

Factors controlling renal excretion of ammonium, hydrogen and potassium.National Science Foundation. #GB-4314. Principal Investigator: LawrenceP. Sullivan. Jan. 1, 1966 - Dec. 31, 1967. $35,400 (for 2 years).

Hydrogen ion retention in diabetic acidosis and ketosis. USPHS #AM-06570.Principal Investigator: Virginia Tucker; Co-Principal Investigator: E. B. Brow n, Jr.Sept. 1, 1965 to Aug. 31, 1966. $10,860. Extended to March 31, 1967 withoutadditional funds.

Right atrial pressure and heart rate. USPHS #1 ROI HE-08803. PrincipalInvestigator: K. L. Goetz. June 1, 1966 to May 31, 1967. $13,300.

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Motor effects of the arrest reaction. USPHS #MH 08908. PrincipalInvestigator: G. N. Loofbourrow. June 1, 1965 to May 31, 1966. $13,678.Extended to May 31, 1967 without additional funds.

Studies on the maternal-fetal relationship in sheep. USPHS #HD-00556.Principal Investigator: A. Stark Wolkoff. Jan. 1, 1966 to Dec. 31, 1966. $13,053.Extended to Dec. 31, 1967 without additional funds.

PHS Training Grant. Program Director: E. B. Brown, Jr. #5-TlGM1l37 -03.July 1, 1966 to June 30, 1967. $41,138.

Diuretic Agents and ion transport in the toad bladder. lROl AM 10227, USPHS.Principal Investigator: Lawrence P. Sullivan. $12,865.

The correlation ofelectrical activity with cardiovascular state. KUMCInstitutional grant #09589094. Principal Investigator: John W. Trank.Oct. 1, 1965 - Sept. 30, 1966. $2,920.

Kansas Heart Association. In vivo cardiac tissue buffering. PrincipalInvestigator: E. B. Brown, Jr. $2,500. July 1, 1966 to June 30, 1967.

Kansas Heart Association. Factors controlling the renal excretion of hydrogenand potassium. Principal Investigator: L. P. Sullivan. July 1, 1965 to June 30,1966. $3,595. Extended to June 30, 1967, without additional funds.

Career Development .Award #5-K3-GM-ll, 898-02.port. Dr. Brown in behalf of L. P. Sullivan.

Renal Tubular potassium trans-

Kansas Heart Association. Coronary blood flow as related to right heart pressure.Principal Investigator: K. L. Goetz. July 1, 1966 to June 30, 1967. $3,000.

Kansas Heart Association Grant #66R-1603, July 1, 1966 to June 30, 1967.Dr. Trank.

Heart ofAmerica United Fund Board, Inc. #66-R-1559, $3,000. - Dr. Goodridge.

Cancer Society Grant #P-426 for Hormonal and nutritional regulation of lipogenesisduring development and growth. $37,380 from 12-1-66 to 11-30-68 - Dr. Goodridge

NIH-Dr. Thompson. USPHS NB 07059-01, Jan. 1, 1966 to Dec. 31, 1967, $21, 250.A. M. Thompson Principal Investigator. Nonelectrolyte Transport Alteration inPerfused Brain.

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Dr. Thompson is one of the Co-Investigators on USPHS #NB-HE 06907 -01.

June 1, 1966 to August 31, 1967, $60,00G. Interdepartmental Project Grant.Prognosis and theory in Cerebrovascular Disease.

American Cancer Society - Experimental Ovarian Tumors.

July 1, 1966 to Dec. 1, 1967 - $15,750. Principal Investigator, Dr. D. C. Johnson.

National Science Foundation - Development of male and female hypophyseal

Gonadotropin patterns. June 1, 1966 to May 31, 1968 - $19, 150. Principal

Investigator Dr. Donald C. Johnson.

Population Council Inc. - Neurohormone - Hypophyseal gonadotropin andsteroid mutations. Jan. 1, 1966 - Dec. 1968 - $20,000 - Dr. Johnson.

PUBLICATIONS

Clancy, R. L. and E. B. Brown, Jr. In vivo CO2 buffer curves of skeletaland cardiac muscle. Am. J. Physiol. 211:1309-1312,1966.

Brown, E. B., Jr. Philosophical or Physiological? The Physiological Process

of Dying- -A Physiologist Looks at Death. The J. Kansas Medical SocietyVol. LXVIII, No. III,127, 1967.

Brown, E. B., Jr., R. L. Clancy, W. G. Kim, and Virginia Tucker,

Quantitative aspects of CO2 buffering of tissue. Am. Review of RespiratoryDiseases. (Inpress)

Clancy, R. L. and E. B. Brown, Jr. Cardiac potassium flux and acid-base

changes (submitted to Am. J. Physiol.)

Goetz, Kenneth L. and Frank F. Hanis. A convenient skin button connector for

withdrawal of blood samples. J. Appl. Physiol. (Inpress)

Goodridge, A. G. and E. G. Ball (1966) Lipogenesis in the pigeon: ~vitrostudies. Am. J. Physiol. 211:803.

Goodridge, A. G. and E. G. Ball (1967) Lipogenesis in the pigeon: ..!E: vivostudies. Am. J. Physiol. (Inpress)

Goodridge, .I. G. and E. G. Ball (1967) The effect of prolactin on lipogenesis

in the pigeon: In vivo studies. Biochemistry. In press.

Goodridge, P. G. and E. G. Ball (1967) The effect of prolactin on lipogenesis

in the pigeon: ~ vitro studies. Biochemistry. In press.

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Johnson, D. C. and:c. M. Nelson. The amount of luteinizing hormone activityin extracts of sheep hypothalamus. Endocrinology 2§:90l-903, 1966.

Johnson, D. C., F. M. Foltz, and D. M. Nelson. Abnormal lactation:experimental production of physiological hypothalamic lesions in sheep.Endocrinology and Metabolism 26:915-917, 1966.

J. Clin.

Johnson, D. C. - An anovulatory bilateral hermaphroditic rat.1287-1288, 1966.

Nature 210:

Johnson, D. C. Gonadotropin Patterns in ffiale and female rats: Plasma LHand FSH at various ages evaluated by the ffiethod of parabiosis. ActaEndocrinologica (in press).

PenCileton, R., L. P. Sullivan, J. Tucker and R. E. Stevenson. The effects ofa Thiazide and a Mercurial Diur etic on the isolated toad bladder. (Submitted toA. J. P.)

Thompson, P. M. (with R. E. Thurber) Stationery-state sodium and potassiumion distribution of human erythrocytes. Am. J. Physiol., 1967, vol 212,887 -883.

Pekas, J. C. , A. M. Thompson, and Virgil W. Hays. Characteristics of theexocrine pancreatic secretion of the young pig. J. Animal Science 25:113-121 (1966)

Wolkoff, A. S., and Arnold, P. G. Fetal lung changes with maternal stress. iI

J. Kansas Med. Society, 67:269, 1966.

Bawden, J. W. and Wolkoff, A. S. Fetal blood calcium responses to maternalcalcium perfusion in sheep. Accepted Am. J. Gyn and Ob., 1966.

Wolkoff, A. S. Bleeding in Late Pregnancy. Hospital Medicine, 3:129, 1967.

Wolkoff, A. S. Lactic and pyruvic acid concentrations in the fetal lamb.To be published.

Wolkoff, P. S. A TP concentration in pregnant and non-pregnant uterinemuscle using firefly lantern. To be published.

Evans, J. A., R. W. Hamilton, Jr., Marian C. Kuenzig, Leonard F. Peltier.Effects of anesthetic agents on surface properties of dipalmitoyl lecithin.J. International Anesthesiology Research Society 45:(3) 28.5-289, 1966.